MD06G1111600.v1.1

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005081
Physical Location & Seq
Forward (+)
25033898 .. 25036424
2527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1111600.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGATCCGAAGCTCACAGAGGTCGCACAGCTCTTCGAGCGATTCAAAGCTGCATTCGTCCGGAACGATTTCGATACCTGCTCCAATTTCCTCTCCCAGCTCAAGGTTAAGCTGACTGAATTCGGAAGCCTACCACCGTTGTTTCAAGACACACCTAATGCTGTCAAGGAATTAACATTAGCAAGGGATATATATGAGCATGCTGTTGTTCTTAGTGTGAAGATTGAGGATCAGGATGCATTTGAAAGGGATTTCTTTCAGTTGAAGTCTTATTACGTAGACGCTCGTGACCGTCTTCCACCATCCCTTCAAGAGTACCCAATTTTAGGTCTCAACCTGTTGAGGCTCCTTGTCCAGAATAGAATTGCTGAGTTCCACACTGAACTGGAACTTCTTTCATCTGCTGCTTTGGAGAACCCTTGCATTAAGCATGCTGTAGAGCTAGAGCAATCTTTCATGGAGGGGGCTTACAACCGTGTGTTGAGCGCTCGTCAGACAGTGCCACACGAGACTTATGTTTATTTCATGGATCTACTCGCTAAAACAGTCAGGGATGAGATAGCCGGATGCAGTGAGAAGGCATATGATTCACTCTCTATTAAGGATGCCCGTCAAATGTTGCTGTTTCCTTCTGACCGCGAACTTTTGGCATATGTCAGTGAGGATCATCCTGAGTGGGAAGTGAAGAATGATGCCGTCTATTTCCAGAAGGCAAAGGAGTCTGCACCTTGCAAAGAGATACCTTCTCTACAACTCATCAACCAAACTCTCAGCTACGCAAGAGAGTTGGAGCGGATTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

268

Amino Acids

30.79

Weight (kDa)

4.93

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CSN8_PSD8_EIF3K PF10075 106 - 242 1.6e-29 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 86
AccBSI CCGCTC 1 cut(s) 793
AccI GTMKAC 1 cut(s) 279
AccII CGCG 1 cut(s) 639
AccIII TCCGGA 1 cut(s) 60
AciI CCGC 2 cut(s) 637, 793
AclWI GGATC 4 cut(s) 12, 237, 537, 672
AcsI RAATTY 1 cut(s) 119
AfaI GTAC 1 cut(s) 317
AfeI AGCGCT 1 cut(s) 487
AfiI CCNNNNNNNGG 2 cut(s) 103, 326
AgsI TTSAA 5 cut(s) 46, 146, 245, 265, 311
AluBI AGCT 7 cut(s) 13, 31, 50, 100, 112, 442, 774
AluI AGCT 7 cut(s) 13, 31, 50, 100, 112, 442, 774
Alw26I GTCTC 2 cut(s) 335, 503
AlwI GGATC 4 cut(s) 12, 237, 537, 672
Aor13HI TCCGGA 1 cut(s) 60
Aor51HI AGCGCT 1 cut(s) 487
ApeKI GCWGC 2 cut(s) 50, 404
ApoI RAATTY 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 488
BamHI GGATCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 257, 289
BauI CACGAG 2 cut(s) 285, 506
BbsI GAAGAC 1 cut(s) 287
BbvI GCAGC 2 cut(s) 37, 391
BccI CCATC 1 cut(s) 310
BceAI ACGGC 1 cut(s) 680
BcoDI GTCTC 2 cut(s) 335, 503
BfaI CTAG 1 cut(s) 443
BfmI CTRYAG 1 cut(s) 435
BfoI RGCGCY 1 cut(s) 489
BfuAI ACCTGC 1 cut(s) 86
BisI GCNGC 2 cut(s) 51, 405
BlsI GCNGC 2 cut(s) 52, 406
BmiI GGNNCC 2 cut(s) 6, 347
BmsI GCATC 4 cut(s) 226, 557, 595, 682
BpiI GAAGAC 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 86
BsaAI YACGTR 1 cut(s) 277
BsaI GGTCTC 1 cut(s) 335
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 326
Bse1I ACTGG 1 cut(s) 390
BseAI TCCGGA 1 cut(s) 60
BseGI GGATG 6 cut(s) 241, 302, 559, 572, 610, 667
BseLI CCNNNNNNNGG 2 cut(s) 103, 326
BseMII CTCAG 3 cut(s) 360, 663, 784
BseNI ACTGG 1 cut(s) 390
BseXI GCAGC 2 cut(s) 37, 391
BseYI CCCAGC 1 cut(s) 96
BsgI GTGCAG 1 cut(s) 708
Bsh1236I CGCG 1 cut(s) 639
BsiSI CCGG 2 cut(s) 61, 564
BslI CCNNNNNNNGG 2 cut(s) 103, 326
BsmAI GTCTC 2 cut(s) 335, 503
BsmI GAATGC 1 cut(s) 53
Bso31I GGTCTC 1 cut(s) 335
Bsp13I TCCGGA 1 cut(s) 60
Bsp143I GATC 4 cut(s) 4, 229, 529, 664
BspACI CCGC 2 cut(s) 637, 793
BspCNI CTCAG 3 cut(s) 361, 664, 783
BspEI TCCGGA 1 cut(s) 60
BspFNI CGCG 1 cut(s) 639
BspLI GGNNCC 2 cut(s) 6, 347
BspMI ACCTGC 1 cut(s) 86
BspPI GGATC 4 cut(s) 12, 237, 537, 672
BspQI GCTCTTC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 335
BsrBI CCGCTC 1 cut(s) 793
BsrI ACTGG 1 cut(s) 390
BssMI GATC 4 cut(s) 4, 229, 529, 664
BssSI CACGAG 2 cut(s) 285, 506
Bst2BI CACGAG 2 cut(s) 285, 506
Bst4CI ACNGT 5 cut(s) 138, 293, 476, 499, 547
Bst6I CTCTTC 1 cut(s) 38
BstBAI YACGTR 1 cut(s) 277
BstC8I GCNNGC 2 cut(s) 201, 432
BstDEI CTNAG 4 cut(s) 212, 369, 672, 770
BstF5I GGATG 6 cut(s) 241, 302, 559, 572, 610, 667
BstFNI CGCG 1 cut(s) 639
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 4 cut(s) 7, 232, 532, 667
BstMAI GTCTC 2 cut(s) 335, 503
BstMBI GATC 4 cut(s) 4, 229, 529, 664
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNSI RCATGY 2 cut(s) 203, 434
BstSFI CTRYAG 1 cut(s) 435
BstSNI TACGTA 1 cut(s) 277
BstUI CGCG 1 cut(s) 639
BstV1I GCAGC 2 cut(s) 37, 391
BstV2I GAAGAC 1 cut(s) 287
BstX2I RGATCY 2 cut(s) 4, 529
BstYI RGATCY 2 cut(s) 4, 529
BtsCI GGATG 6 cut(s) 241, 302, 559, 572, 610, 667
BtsI GCAGTG 1 cut(s) 577
BtsIMutI CAGTG 4 cut(s) 378, 504, 577, 664
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 2 cut(s) 201, 432
CfoI GCGC 1 cut(s) 488
CseI GACGC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 316
CviAII CATG 4 cut(s) 200, 431, 457, 526
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 4 cut(s) 212, 369, 672, 770
DpnI GATC 4 cut(s) 6, 231, 531, 666
DpnII GATC 4 cut(s) 4, 229, 529, 664
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco105I TACGTA 1 cut(s) 277
Eco31I GGTCTC 1 cut(s) 335
Eco47III AGCGCT 1 cut(s) 487
EcoRI GAATTC 1 cut(s) 119
EcoT22I ATGCAT 1 cut(s) 241
FaeI CATG 4 cut(s) 203, 434, 460, 529
FatI CATG 4 cut(s) 199, 430, 456, 525
FauNDI CATATG 2 cut(s) 583, 652
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 2 cut(s) 51, 405
FokI GGATG 6 cut(s) 248, 289, 566, 579, 617, 654
Fsp4HI GCNGC 2 cut(s) 51, 405
FspBI CTAG 1 cut(s) 443
GlaI GCGC 1 cut(s) 487
GluI GCNGC 2 cut(s) 51, 405
GsaI CCCAGC 1 cut(s) 100
HaeII RGCGCY 1 cut(s) 489
HapII CCGG 2 cut(s) 61, 564
HgaI GACGC 1 cut(s) 290
HhaI GCGC 1 cut(s) 488
Hin1II CATG 4 cut(s) 203, 434, 460, 529
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HinfI GANTC 3 cut(s) 42, 587, 719
HpaII CCGG 2 cut(s) 61, 564
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 4 cut(s) 9, 125, 495, 634
Hpy188III TCNNGA 8 cut(s) 61, 146, 233, 287, 311, 355, 671, 706
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 5 cut(s) 317, 571, 639, 703, 753
HpyCH4III ACNGT 5 cut(s) 138, 293, 476, 499, 547
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 6 cut(s) 53, 239, 423, 570, 725, 732
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 4 cut(s) 212, 369, 672, 770
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 4 cut(s) 203, 434, 460, 529
HspAI GCGC 1 cut(s) 486
Kpn2I TCCGGA 1 cut(s) 60
Kzo9I GATC 4 cut(s) 4, 229, 529, 664
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 3 cut(s) 86, 351, 790
Lsp1109I GCAGC 2 cut(s) 37, 391
LweI GCATC 4 cut(s) 226, 557, 595, 682
MaeI CTAG 1 cut(s) 443
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 287
MalI GATC 4 cut(s) 6, 231, 531, 666
MbiI CCGCTC 1 cut(s) 793
MboI GATC 4 cut(s) 4, 229, 529, 664
MboII GAAGA 4 cut(s) 25, 232, 287, 697
MflI RGATCY 2 cut(s) 4, 529
MluCI AATT 5 cut(s) 85, 119, 170, 321, 363
MlyI GAGTC 1 cut(s) 728
MmeI TCCRAC 1 cut(s) 768
MnlI CCTC 6 cut(s) 13, 101, 220, 336, 454, 655
Mph1103I ATGCAT 1 cut(s) 241
MroI TCCGGA 1 cut(s) 60
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 4 cut(s) 108, 173, 426, 600
MspI CCGG 2 cut(s) 61, 564
Mva1269I GAATGC 1 cut(s) 53
MvnI CGCG 1 cut(s) 639
MwoI GCNNNNNNNGC 1 cut(s) 37
NdeI CATATG 2 cut(s) 583, 652
NdeII GATC 4 cut(s) 4, 229, 529, 664
NlaIII CATG 4 cut(s) 203, 434, 460, 529
NlaIV GGNNCC 2 cut(s) 6, 347
NmuCI GTSAC 1 cut(s) 287
NsiI ATGCAT 1 cut(s) 241
NspI RCATGY 2 cut(s) 203, 434
PaeI GCATGC 2 cut(s) 203, 434
PciSI GCTCTTC 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 53
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 2 cut(s) 42, 587
PkrI GCNGC 2 cut(s) 52, 406
PleI GAGTC 1 cut(s) 727
PpsI GAGTC 1 cut(s) 727
Ppu21I YACGTR 1 cut(s) 277
PspFI CCCAGC 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 6, 347
PsuI RGATCY 2 cut(s) 4, 529
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 4 cut(s) 108, 173, 426, 600
SatI GCNGC 2 cut(s) 51, 405
Sau3AI GATC 4 cut(s) 4, 229, 529, 664
SchI GAGTC 1 cut(s) 728
SfaNI GCATC 4 cut(s) 226, 557, 595, 682
SfcI CTRYAG 1 cut(s) 435
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SnaBI TACGTA 1 cut(s) 277
SphI GCATGC 2 cut(s) 203, 434
Sse9I AATT 5 cut(s) 85, 119, 170, 321, 363
SsiI CCGC 2 cut(s) 637, 793
SspMI CTAG 1 cut(s) 443
TaaI ACNGT 5 cut(s) 138, 293, 476, 499, 547
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 36, 72
TasI AATT 5 cut(s) 85, 119, 170, 321, 363
TfiI GAWTC 2 cut(s) 42, 587
Tru1I TTAA 4 cut(s) 108, 173, 426, 600
Tru9I TTAA 4 cut(s) 108, 173, 426, 600
TscAI CASTG 4 cut(s) 385, 504, 577, 664
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 2 cut(s) 50, 404
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 3 cut(s) 387, 445, 514
TspRI CASTG 4 cut(s) 385, 504, 577, 664
XapI RAATTY 1 cut(s) 119
XceI RCATGY 2 cut(s) 203, 434
XmiI GTMKAC 1 cut(s) 279
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 1 cut(s) 443
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.