AT1G64710

Alcohol dehydrogenase GroES-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005326
Physical Location & Seq
Forward (+)
24044686 .. 24046621
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64710.3

Sequence Viewer

Length: 1143 bp
ATGGAAACACAAGGCAAAGTAATCACTTGCAAAGCTGCTGTGGCATGGGGAGCTGGAGAGCCTCTAGTAATGGAAGATGTAAAAGTGGATCCTCCTCAAAGATTGGAAGTGAGAATTCGAATCCTCTTCACTTCCATCTGTCACACTGATCTCAGCGCATGGAAAGGCGAGAACGAGGCTCAGCGAGCATACCCTCGAATCCTTGGCCATGAGGCAGCAGGGATAGTGGAGAGCGTAGGGGAAGGTGTGGAAGAGATGATGGCAGGAGATCATGTGCTCCCTATTTTCACAGGAGAGTGTGGAGACTGCAGAGTGTGCAAGCGAGACGGAGCCAATCTGTGCGAGAGATTCCGAGTGGATCCGATGAAGAAGGTGATGGTAACCGATGGAAAGACCAGATTCTTCACCAGCAAAGACAACAAACCAATCTACCATTTTCTCAACACATCCACCTTCTCCGAGTACACGGTCATAGACTCGGCCTGCGTGCTCAAGGTGGACCCTCTGTTTCCTCTGGAGAAGATAAGCCTCCTCAGCTGCGGTGTCTCCACTGGAGTCGGTGCGGCGTGGAATGTGGCTGATATTCAACCTGCCTCCACCGTCGCTATTTTTGGACTTGGCGCTGTTGGACTTGCCGTGGCTGAAGGTGCAAGAGCCAGAGGAGCCTCTAAAATCATTGGGATCGACATCAACCCTGACAAGTTCCAACTAGGCAGAGAAGCAGGGATTAGTGAATTCATAAATCCAAAGGAGAGTGACAAGGCAGTTCATGAGAGAGTAATGGAGATAACAGAGGGAGGAGTGGAGTACAGCTTCGAGTGTGCAGGAAGTATAGAAGCACTTAGAGAAGCCTTTCTGTCTACAAACTCAGGAGTGGGAGTGACTGTCATGCTGGGCGTTCACGCAAGCCCACAGCTTCTCCCTATCCATCCCATGGAGCTCTTCCAAGGCAGATCCATCACCGCCTCTGTTTTCGGAGGCTTCAAACCCAAAACCCAATTACCCTTTTTCATCACCCAGTGCCTCCAAGGTCTTCTCAATCTCGATCTCTTCATCTCTCACCAGCTTCCCTTCCACGATATCAACGAAGCCATGCAGCTGCTTCACCAAGGCAAAGCTCTTCGTTGCCTTCTCCACCTCTAA

Protein Analysis

380

Amino Acids

41.32

Weight (kDa)

5.7

Isoelectric Point (pI)

24.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 139 - 258 3e-21 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 598
AccB7I CCANNNNNTGG 1 cut(s) 934
AccI GTMKAC 1 cut(s) 860
AciI CCGC 3 cut(s) 540, 563, 963
AclWI GGATC 6 cut(s) 83, 96, 353, 366, 689, 948
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 2 cut(s) 114, 734
AcuI CTGAAG 1 cut(s) 663
AdeI CACNNNGTG 1 cut(s) 1020
AfaI GTAC 2 cut(s) 464, 809
AfiI CCNNNNNNNGG 1 cut(s) 934
AgsI TTSAA 2 cut(s) 587, 985
AluBI AGCT 9 cut(s) 35, 53, 537, 813, 916, 940, 1066, 1099, 1118
AluI AGCT 9 cut(s) 35, 53, 537, 813, 916, 940, 1066, 1099, 1118
Alw21I GWGCWC 3 cut(s) 279, 492, 942
Alw26I GTCTC 3 cut(s) 297, 318, 550
AlwI GGATC 6 cut(s) 83, 96, 353, 366, 689, 948
AoxI GGCC 2 cut(s) 205, 480
ApeKI GCWGC 5 cut(s) 35, 215, 537, 1096, 1099
ApoI RAATTY 2 cut(s) 114, 734
Asp700I GAANNNNTTC 1 cut(s) 852
AspLEI GCGC 2 cut(s) 158, 623
AspS9I GGNCC 1 cut(s) 499
AsuHPI GGTGA 6 cut(s) 385, 397, 952, 1006, 1052, 1097
AsuII TTCGAA 1 cut(s) 118
AvaII GGWCC 1 cut(s) 499
BalI TGGCCA 1 cut(s) 207
BamHI GGATCC 2 cut(s) 88, 358
BanII GRGCYC 1 cut(s) 942
BbsI GAAGAC 1 cut(s) 1025
Bbv12I GWGCWC 3 cut(s) 279, 492, 942
BbvCI CCTCAGC 1 cut(s) 533
BbvI GCAGC 5 cut(s) 22, 227, 524, 1086, 1108
BccI CCATC 6 cut(s) 143, 253, 370, 380, 936, 965
BceAI ACGGC 1 cut(s) 620
BcoDI GTCTC 3 cut(s) 297, 318, 550
BfaI CTAG 2 cut(s) 65, 710
BfmI CTRYAG 1 cut(s) 307
BfoI RGCGCY 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 598
BisI GCNGC 6 cut(s) 36, 216, 538, 564, 1097, 1100
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 6 cut(s) 37, 217, 539, 565, 1098, 1101
Bme18I GGWCC 1 cut(s) 499
BmgT120I GGNCC 1 cut(s) 499
BmiI GGNNCC 5 cut(s) 90, 331, 360, 501, 664
BmrI ACTGGG 1 cut(s) 1012
BmuI ACTGGG 1 cut(s) 1012
BpiI GAAGAC 1 cut(s) 1025
BpmI CTGGAG 3 cut(s) 75, 536, 573
Bpu10I CCTNAGC 1 cut(s) 533
Bpu1102I GCTNAGC 1 cut(s) 180
Bpu14I TTCGAA 1 cut(s) 118
BpuEI CTTGAG 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 686
BsaJI CCNNGG 6 cut(s) 202, 636, 933, 946, 1027, 1108
BsaXI ACNNNNNCTCC 8 cut(s) 258, 288, 864, 870, 894, 900, 903, 933
Bsc4I CCNNNNNNNGG 1 cut(s) 934
Bse1I ACTGG 2 cut(s) 556, 1018
Bse8I GATNNNNATC 1 cut(s) 686
BseDI CCNNGG 6 cut(s) 202, 636, 933, 946, 1027, 1108
BseGI GGATG 2 cut(s) 446, 928
BseJI GATNNNNATC 1 cut(s) 686
BseLI CCNNNNNNNGG 1 cut(s) 934
BseMII CTCAG 4 cut(s) 166, 194, 547, 882
BseNI ACTGG 2 cut(s) 556, 1018
BseRI GAGGAG 4 cut(s) 84, 521, 675, 813
BseXI GCAGC 5 cut(s) 22, 227, 524, 1086, 1108
BseYI CCCAGC 1 cut(s) 892
BsgI GTGCAG 1 cut(s) 843
BshFI GGCC 2 cut(s) 207, 482
BsiHKAI GWGCWC 3 cut(s) 279, 492, 942
BslI CCNNNNNNNGG 1 cut(s) 934
BsmAI GTCTC 3 cut(s) 297, 318, 550
BsmBI CGTCTC 1 cut(s) 318
BsnI GGCC 2 cut(s) 207, 482
Bsp119I TTCGAA 1 cut(s) 118
Bsp1286I GDGCHC 3 cut(s) 279, 492, 942
Bsp143I GATC 7 cut(s) 88, 148, 268, 358, 681, 953, 1045
Bsp1720I GCTNAGC 1 cut(s) 180
Bsp19I CCATGG 1 cut(s) 933
BspACI CCGC 3 cut(s) 540, 563, 963
BspANI GGCC 2 cut(s) 207, 482
BspCNI CTCAG 4 cut(s) 165, 193, 546, 881
BspHI TCATGA 1 cut(s) 769
BspLI GGNNCC 5 cut(s) 90, 331, 360, 501, 664
BspMAI CTGCAG 1 cut(s) 311
BspMI ACCTGC 1 cut(s) 598
BspPI GGATC 6 cut(s) 83, 96, 353, 366, 689, 948
BspQI GCTCTTC 2 cut(s) 947, 1125
BspT104I TTCGAA 1 cut(s) 118
BsrI ACTGG 2 cut(s) 556, 1018
BssECI CCNNGG 6 cut(s) 202, 636, 933, 946, 1027, 1108
BssMI GATC 7 cut(s) 88, 148, 268, 358, 681, 953, 1045
BssT1I CCWWGG 5 cut(s) 202, 933, 946, 1027, 1108
Bst4CI ACNGT 3 cut(s) 469, 601, 886
Bst6I CTCTTC 5 cut(s) 131, 246, 947, 1055, 1125
BstAPI GCANNNNNTGC 1 cut(s) 315
BstBI TTCGAA 1 cut(s) 118
BstC8I GCNNGC 5 cut(s) 186, 320, 484, 488, 907
BstDEI CTNAG 5 cut(s) 152, 180, 533, 842, 868
BstDSI CCRYGG 2 cut(s) 636, 933
BstEII GGTNACC 1 cut(s) 379
BstF5I GGATG 2 cut(s) 446, 928
BstH2I RGCGCY 1 cut(s) 624
BstHHI GCGC 2 cut(s) 158, 623
BstKTI GATC 7 cut(s) 91, 151, 271, 361, 684, 956, 1048
BstMAI GTCTC 3 cut(s) 297, 318, 550
BstMBI GATC 7 cut(s) 88, 148, 268, 358, 681, 953, 1045
BstMWI GCNNNNNNNGC 7 cut(s) 41, 50, 185, 315, 534, 647, 662
BstPI GGTNACC 1 cut(s) 379
BstSFI CTRYAG 1 cut(s) 307
BstV1I GCAGC 5 cut(s) 22, 227, 524, 1086, 1108
BstV2I GAAGAC 1 cut(s) 1025
BstX2I RGATCY 3 cut(s) 88, 358, 953
BstYI RGATCY 3 cut(s) 88, 358, 953
BsuRI GGCC 2 cut(s) 207, 482
BtgI CCRYGG 2 cut(s) 636, 933
BtsCI GGATG 2 cut(s) 446, 928
BtsIMutI CAGTG 3 cut(s) 144, 549, 1025
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 5 cut(s) 186, 320, 484, 488, 907
CciI TCATGA 1 cut(s) 769
CfoI GCGC 2 cut(s) 158, 623
Cfr13I GGNCC 1 cut(s) 499
Csp6I GTAC 2 cut(s) 463, 808
CviAII CATG 8 cut(s) 45, 159, 209, 272, 770, 889, 934, 1093
CviQI GTAC 2 cut(s) 463, 808
DdeI CTNAG 5 cut(s) 152, 180, 533, 842, 868
DpnI GATC 7 cut(s) 90, 150, 270, 360, 683, 955, 1047
DpnII GATC 7 cut(s) 88, 148, 268, 358, 681, 953, 1045
DraIII CACNNNGTG 1 cut(s) 1020
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 5 cut(s) 131, 246, 947, 1055, 1125
EarI CTCTTC 5 cut(s) 131, 246, 947, 1055, 1125
Ecl136II GAGCTC 1 cut(s) 940
Eco130I CCWWGG 5 cut(s) 202, 933, 946, 1027, 1108
Eco24I GRGCYC 1 cut(s) 942
Eco32I GATATC 1 cut(s) 1081
Eco47I GGWCC 1 cut(s) 499
Eco53kI GAGCTC 1 cut(s) 940
Eco57I CTGAAG 1 cut(s) 663
Eco91I GGTNACC 1 cut(s) 379
EcoICRI GAGCTC 1 cut(s) 940
EcoO65I GGTNACC 1 cut(s) 379
EcoRI GAATTC 2 cut(s) 114, 734
EcoRV GATATC 1 cut(s) 1081
EcoT14I CCWWGG 5 cut(s) 202, 933, 946, 1027, 1108
EcoT38I GRGCYC 1 cut(s) 942
ErhI CCWWGG 5 cut(s) 202, 933, 946, 1027, 1108
Esp3I CGTCTC 1 cut(s) 318
FaeI CATG 8 cut(s) 48, 162, 212, 275, 773, 892, 937, 1096
FatI CATG 8 cut(s) 44, 158, 208, 271, 769, 888, 933, 1092
FblI GTMKAC 1 cut(s) 860
Fnu4HI GCNGC 6 cut(s) 36, 216, 538, 564, 1097, 1100
FokI GGATG 2 cut(s) 433, 915
FriOI GRGCYC 1 cut(s) 942
Fsp4HI GCNGC 6 cut(s) 36, 216, 538, 564, 1097, 1100
FspBI CTAG 2 cut(s) 65, 710
GlaI GCGC 2 cut(s) 157, 622
GluI GCNGC 6 cut(s) 36, 216, 538, 564, 1097, 1100
GsaI CCCAGC 1 cut(s) 896
GsuI CTGGAG 3 cut(s) 75, 536, 573
HaeII RGCGCY 1 cut(s) 624
HaeIII GGCC 2 cut(s) 207, 482
HhaI GCGC 2 cut(s) 158, 623
Hin1II CATG 8 cut(s) 48, 162, 212, 275, 773, 892, 937, 1096
Hin6I GCGC 2 cut(s) 156, 621
HinP1I GCGC 2 cut(s) 156, 621
HinfI GANTC 6 cut(s) 120, 198, 348, 399, 476, 555
HphI GGTGA 6 cut(s) 385, 397, 952, 1006, 1052, 1097
Hpy166II GTNNAC 4 cut(s) 465, 499, 861, 901
Hpy188I TCNGA 4 cut(s) 353, 363, 460, 977
Hpy188III TCNNGA 4 cut(s) 515, 770, 870, 1043
Hpy8I GTNNAC 4 cut(s) 465, 499, 861, 901
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 6 cut(s) 236, 364, 463, 638, 1081, 1139
HpyCH4III ACNGT 3 cut(s) 469, 601, 886
HpyCH4V TGCA 6 cut(s) 30, 309, 318, 650, 824, 1096
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 50, 185, 315, 534, 647, 662
HpyF3I CTNAG 5 cut(s) 152, 180, 533, 842, 868
Hsp92II CATG 8 cut(s) 48, 162, 212, 275, 773, 892, 937, 1096
HspAI GCGC 2 cut(s) 156, 621
Kzo9I GATC 7 cut(s) 88, 148, 268, 358, 681, 953, 1045
LguI GCTCTTC 2 cut(s) 947, 1125
LmnI GCTCC 5 cut(s) 50, 282, 329, 662, 937
Lsp1109I GCAGC 5 cut(s) 22, 227, 524, 1086, 1108
MaeI CTAG 2 cut(s) 65, 710
MaeIII GTNAC 4 cut(s) 140, 379, 755, 880
MalI GATC 7 cut(s) 90, 150, 270, 360, 683, 955, 1047
MboI GATC 7 cut(s) 88, 148, 268, 358, 681, 953, 1045
MflI RGATCY 3 cut(s) 88, 358, 953
MhlI GDGCHC 3 cut(s) 279, 492, 942
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 3 cut(s) 114, 734, 998
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 2 cut(s) 470, 564
MmeI TCCRAC 2 cut(s) 607, 730
Mox20I TGGCCA 1 cut(s) 207
MroXI GAANNNNTTC 1 cut(s) 852
MscI TGGCCA 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 2 cut(s) 537, 1099
MwoI GCNNNNNNNGC 7 cut(s) 41, 50, 185, 315, 534, 647, 662
NcoI CCATGG 1 cut(s) 933
NdeII GATC 7 cut(s) 88, 148, 268, 358, 681, 953, 1045
NlaIII CATG 8 cut(s) 48, 162, 212, 275, 773, 892, 937, 1096
NlaIV GGNNCC 5 cut(s) 90, 331, 360, 501, 664
NmeAIII GCCGAG 1 cut(s) 458
NmuCI GTSAC 3 cut(s) 140, 755, 880
NspV TTCGAA 1 cut(s) 118
PagI TCATGA 1 cut(s) 769
PciSI GCTCTTC 2 cut(s) 947, 1125
PcsI WCGNNNNNNNCGW 1 cut(s) 1083
PdmI GAANNNNTTC 1 cut(s) 852
PfeI GAWTC 4 cut(s) 120, 198, 348, 399
PflMI CCANNNNNTGG 1 cut(s) 934
PkrI GCNGC 6 cut(s) 37, 217, 539, 565, 1098, 1101
PleI GAGTC 2 cut(s) 470, 563
PpsI GAGTC 2 cut(s) 470, 563
Psp124BI GAGCTC 1 cut(s) 942
PspEI GGTNACC 1 cut(s) 379
PspFI CCCAGC 1 cut(s) 892
PspN4I GGNNCC 5 cut(s) 90, 331, 360, 501, 664
PspPI GGNCC 1 cut(s) 499
PstI CTGCAG 1 cut(s) 311
PsuI RGATCY 3 cut(s) 88, 358, 953
PvuII CAGCTG 2 cut(s) 537, 1099
RsaI GTAC 2 cut(s) 464, 809
RsaNI GTAC 2 cut(s) 463, 808
SacI GAGCTC 1 cut(s) 942
SapI GCTCTTC 2 cut(s) 947, 1125
SatI GCNGC 6 cut(s) 36, 216, 538, 564, 1097, 1100
Sau3AI GATC 7 cut(s) 88, 148, 268, 358, 681, 953, 1045
Sau96I GGNCC 1 cut(s) 499
SchI GAGTC 2 cut(s) 470, 564
SduI GDGCHC 3 cut(s) 279, 492, 942
SfcI CTRYAG 1 cut(s) 307
SfuI TTCGAA 1 cut(s) 118
SinI GGWCC 1 cut(s) 499
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
Sse9I AATT 3 cut(s) 114, 734, 998
SsiI CCGC 3 cut(s) 540, 563, 963
SspMI CTAG 2 cut(s) 65, 710
SstI GAGCTC 1 cut(s) 942
StyI CCWWGG 5 cut(s) 202, 933, 946, 1027, 1108
TaaI ACNGT 3 cut(s) 469, 601, 886
TaqI TCGA 5 cut(s) 118, 196, 684, 816, 1044
TasI AATT 3 cut(s) 114, 734, 998
TatI WGTACW 2 cut(s) 462, 807
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 4 cut(s) 120, 198, 348, 399
TscAI CASTG 3 cut(s) 151, 556, 1025
TseFI GTSAC 3 cut(s) 140, 755, 880
TseI GCWGC 5 cut(s) 35, 215, 537, 1096, 1099
Tsp45I GTSAC 3 cut(s) 140, 755, 880
TspDTI ATGAA 5 cut(s) 380, 727, 758, 1000, 1042
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 3 cut(s) 151, 556, 1025
Van91I CCANNNNNTGG 1 cut(s) 934
VpaK11BI GGWCC 1 cut(s) 499
XapI RAATTY 2 cut(s) 114, 734
XmiI GTMKAC 1 cut(s) 860
XmnI GAANNNNTTC 1 cut(s) 852
XspI CTAG 2 cut(s) 65, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.