AT1G66340

May act early in the ethylene signal transduction pathway, possibly as an ethylene receptor, or as a regulator of the pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003094
Physical Location & Seq
Forward (+)
24734162 .. 24737756
3595 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66340.1

Sequence Viewer

Length: 2217 bp
ATGGAAGTCTGCAATTGTATTGAACCGCAATGGCCAGCGGATGAATTGTTAATGAAATACCAATACATCTCCGATTTCTTCATTGCGATTGCGTATTTTTCGATTCCTCTTGAGTTGATTTACTTTGTGAAGAAATCAGCCGTGTTTCCGTATAGATGGGTACTTGTTCAGTTTGGTGCTTTTATCGTTCTTTGTGGAGCAACTCATCTTATTAACTTATGGACTTTCACTACGCATTCGAGAACCGTGGCGCTTGTGATGACTACCGCGAAGGTGTTAACCGCTGTTGTCTCGTGTGCTACTGCGTTGATGCTTGTTCATATTATTCCTGATCTTTTGAGTGTTAAGACTCGGGAGCTTTTCTTGAAAAATAAAGCTGCTGAGCTCGATAGAGAAATGGGATTGATTCGAACTCAGGAAGAAACCGGAAGGCATGTGAGAATGTTGACTCATGAGATTAGAAGCACTTTAGATAGACATACTATTTTAAAGACTACACTTGTTGAGCTTGGTAGGACATTAGCTTTGGAGGAGTGTGCATTGTGGATGCCTACTAGAACTGGGTTAGAGCTACAGCTTTCTTATACACTTCGTCATCAACATCCCGTGGAGTATACGGTTCCTATTCAATTACCGGTGATTAACCAAGTGTTTGGTACTAGTAGGGCTGTAAAAATATCTCCTAATTCTCCTGTGGCTAGGTTGAGACCTGTTTCTGGGAAATATATGCTAGGGGAGGTGGTCGCTGTGAGGGTTCCGCTTCTCCACCTTTCTAATTTTCAGATTAATGACTGGCCTGAGCTTTCAACAAAGAGATATGCTTTGATGGTTTTGATGCTTCCTTCAGATAGTGCAAGGCAATGGCATGTCCATGAGTTGGAACTCGTTGAAGTCGTCGCTGATCAGGTGGCTGTAGCTCTCTCACATGCTGCGATCCTAGAAGAGTCGATGCGAGCTAGGGACCTTCTCATGGAGCAGAATGTTGCTCTTGATCTAGCTAGACGAGAAGCAGAAACAGCAATCCGTGCCCGCAATGATTTCCTAGCGGTTATGAACCATGAAATGCGAACACCGATGCATGCGATTATTGCACTCTCTTCCTTACTCCAAGAAACGGAACTAACCCCTGAACAAAGACTGATGGTGGAAACAATACTTAAAAGTAGTAACCTTTTGGCAACTTTGATGAATGATGTCTTAGATCTTTCAAGGTTAGAAGATGGAAGTCTTCAACTTGAACTTGGGACATTCAATCTTCATACATTATTTAGAGAGGTCCTCAATCTGATAAAGCCTATAGCGGTTGTTAAGAAATTACCCATCACACTAAATCTTGCACCAGATTTGCCAGAATTTGTTGTTGGGGATGAGAAACGGCTAATGCAGATAATATTAAATATAGTTGGTAATGCTGTGAAATTCTCCAAACAAGGTAGTATCTCCGTAACCGCTCTTGTCACCAAGTCAGACACACGAGCTGCTGACTTTTTTGTCGTGCCAACTGGGAGTCATTTCTACTTGAGAGTGAAGGTAAAAGACTCTGGAGCAGGAATAAATCCTCAAGACATTCCAAAGATTTTCACTAAATTTGCTCAAACACAATCTTTAGCGACGAGAAGCTCGGGTGGTAGTGGGCTTGGCCTCGCCATCTCCAAGAGGTTTGTGAATCTGATGGAGGGTAACATTTGGATTGAGAGCGATGGTCTTGGAAAAGGATGCACGGCTATCTTTGATGTTAAACTTGGGATCTCAGAACGTTCAAACGAATCTAAACAGTCGGGCATACCGAAAGTTCCAGCCATTCCCCGACATTCAAATTTCACTGGACTTAAGGTTCTTGTCATGGATGAGAACGGGGTAAGTAGAATGGTGACGAAGGGACTTCTTGTACACCTTGGGTGCGAAGTGACCACGGTGAGTTCAAACGAGGAGTGTCTCCGAGTTGTGTCCCATGAGCACAAAGTGGTCTTCATGGACGTGTGCATGCCCGGGGTCGAAAACTACCAAATCGCTCTCCGTATTCACGAGAAATTCACAAAACAACGCCACCAACGGCCACTACTTGTGGCACTCAGTGGTAACACTGACAAATCCACAAAAGAGAAATGCATGAGCTTTGGTCTAGACGGTGTGTTGCTCAAACCCGTATCACTAGACAACATAAGAGATGTTCTGTCTGATCTTCTCGAGCCCCGGGTACTGTACGAGGGCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009308 GO:0009408 GO:0009593 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009651 GO:0009690 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009733 GO:0009737 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010087 GO:0010104 GO:0010105 GO:0010119 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016999 GO:0017000 GO:0017144 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0023051 GO:0023052 GO:0023057 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0033993 GO:0034754 GO:0035556 GO:0036211 GO:0038023 GO:0038199 GO:0042221 GO:0042445 GO:0042545 GO:0042562 GO:0042742 GO:0042743 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046483 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048856 GO:0050665 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051239 GO:0051301 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0051740 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0072328 GO:0072593 GO:0097305 GO:0098542 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1903409 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
GAF HATPase_c HisKA Response_reg

Protein Analysis

738

Amino Acids

82.56

Weight (kDa)

7.66

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETR1_N PF25487 17 - 115 1e-39 Ethylene receptor 1-like, N-terminal domain
GAF PF01590 158 - 307 2.4e-12 GAF domain
HisKA PF00512 343 - 408 3.4e-18 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 455 - 581 7.6e-25 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 612 - 724 1.6e-12 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1490
AccB7I CCANNNNNTGG 1 cut(s) 877
AccBSI CCGCTC 1 cut(s) 1451
AccI GTMKAC 1 cut(s) 614
AccII CGCG 1 cut(s) 269
AciI CCGC 9 cut(s) 26, 38, 267, 282, 758, 1030, 1046, 1301, 1449
AclI AACGTT 1 cut(s) 1756
AclWI GGATC 2 cut(s) 928, 1754
AcoI YGGCCR 2 cut(s) 32, 2054
AcsI RAATTY 5 cut(s) 1352, 1418, 1586, 1816, 2030
AcuI CTGAAG 1 cut(s) 828
AdeI CACNNNGTG 2 cut(s) 1963, 2075
AfaI GTAC 5 cut(s) 162, 658, 1890, 2199, 2204
AfiI CCNNNNNNNGG 4 cut(s) 716, 877, 970, 1114
AflII CTTAAG 1 cut(s) 1829
AflIII ACRYGT 1 cut(s) 1977
AgeI ACCGGT 1 cut(s) 634
AhlI ACTAGT 1 cut(s) 659
AjiI CACGTC 1 cut(s) 1978
AjuI GAANNNNNNNTTGG 2 cut(s) 1344, 1376
AloI GAACNNNNNNTCC 2 cut(s) 1818, 1850
Alw21I GWGCWC 2 cut(s) 387, 1959
Alw26I GTCTC 3 cut(s) 295, 700, 1940
AlwI GGATC 2 cut(s) 928, 1754
Ama87I CYCGRG 5 cut(s) 351, 1621, 1988, 2186, 2193
AoxI GGCC 4 cut(s) 32, 794, 1639, 2054
ApeKI GCWGC 3 cut(s) 377, 929, 1478
ApoI RAATTY 5 cut(s) 1352, 1418, 1586, 1816, 2030
ArsI GACNNNNNNTTYG 2 cut(s) 508, 540
AseI ATTAAT 1 cut(s) 786
AsiGI ACCGGT 1 cut(s) 634
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 2 cut(s) 961, 1276
AsuC2I CCSGG 4 cut(s) 1989, 1990, 2194, 2195
AsuHPI GGTGA 4 cut(s) 649, 1450, 1882, 1927
AsuII TTCGAA 1 cut(s) 409
AvaI CYCGRG 5 cut(s) 351, 1621, 1988, 2186, 2193
AvaII GGWCC 2 cut(s) 961, 1276
BaeGI GKGCMC 1 cut(s) 1030
BalI TGGCCA 1 cut(s) 34
BanII GRGCYC 2 cut(s) 387, 2193
BauI CACGAG 3 cut(s) 292, 1473, 2024
BbsI GAAGAC 2 cut(s) 1220, 1960
Bbv12I GWGCWC 2 cut(s) 387, 1959
BbvI GCAGC 3 cut(s) 364, 916, 1465
BccI CCATC 8 cut(s) 150, 820, 1135, 1214, 1328, 1655, 1666, 1694
BceAI ACGGC 4 cut(s) 125, 1391, 1737, 2069
BclI TGATCA 1 cut(s) 901
BcnI CCSGG 4 cut(s) 1989, 1990, 2194, 2195
BcoDI GTCTC 3 cut(s) 295, 700, 1940
BcuI ACTAGT 1 cut(s) 659
BfmI CTRYAG 3 cut(s) 572, 912, 1296
BfoI RGCGCY 1 cut(s) 254
BfrI CTTAAG 1 cut(s) 1829
BglII AGATCT 1 cut(s) 1201
BisI GCNGC 3 cut(s) 378, 930, 1479
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 3 cut(s) 379, 931, 1480
Bme1390I CCNGG 4 cut(s) 1989, 1990, 2194, 2195
Bme18I GGWCC 2 cut(s) 961, 1276
BmeT110I CYCGRG 5 cut(s) 351, 1621, 1988, 2186, 2193
BmgBI CACGTC 1 cut(s) 1978
BmgT120I GGNCC 2 cut(s) 961, 1276
BmiI GGNNCC 3 cut(s) 621, 756, 962
BmrFI CCNGG 4 cut(s) 1989, 1990, 2194, 2195
BmrI ACTGGG 2 cut(s) 570, 1512
BmsI GCATC 6 cut(s) 300, 537, 825, 939, 1065, 1706
BmuI ACTGGG 2 cut(s) 570, 1512
BpiI GAAGAC 2 cut(s) 1220, 1960
BplI GAGNNNNNCTC 2 cut(s) 1263, 1295
BpmI CTGGAG 1 cut(s) 1563
Bpu10I CCTNAGC 1 cut(s) 798
Bpu1102I GCTNAGC 1 cut(s) 381
Bpu14I TTCGAA 1 cut(s) 409
BpuEI CTTGAG 3 cut(s) 131, 1540, 1545
BpuMI CCSGG 4 cut(s) 1989, 1990, 2194, 2195
BsaI GGTCTC 1 cut(s) 700
BsaJI CCNNGG 8 cut(s) 246, 606, 1894, 1911, 1988, 1989, 2192, 2193
BsaWI WCCGGW 2 cut(s) 425, 634
Bsc4I CCNNNNNNNGG 4 cut(s) 716, 877, 970, 1114
Bse118I RCCGGY 1 cut(s) 634
Bse1I ACTGG 4 cut(s) 565, 797, 1507, 1828
Bse3DI GCAATG 4 cut(s) 35, 81, 866, 1039
BseDI CCNNGG 8 cut(s) 246, 606, 1894, 1911, 1988, 1989, 2192, 2193
BseGI GGATG 6 cut(s) 46, 552, 601, 1372, 1721, 1852
BseLI CCNNNNNNNGG 4 cut(s) 716, 877, 970, 1114
BseMI GCAATG 4 cut(s) 35, 81, 866, 1039
BseMII CTCAG 5 cut(s) 372, 428, 789, 1764, 2086
BseNI ACTGG 4 cut(s) 565, 797, 1507, 1828
BseRI GAGGAG 2 cut(s) 545, 1943
BseSI GKGCMC 1 cut(s) 1030
BseXI GCAGC 3 cut(s) 364, 916, 1465
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 4 cut(s) 34, 796, 1641, 2056
BshTI ACCGGT 1 cut(s) 634
BsiHKAI GWGCWC 2 cut(s) 387, 1959
BsiHKCI CYCGRG 5 cut(s) 351, 1621, 1988, 2186, 2193
BsiSI CCGG 4 cut(s) 426, 635, 1989, 2194
BslFI GGGAC 4 cut(s) 974, 1258, 1893, 1933
BslI CCNNNNNNNGG 4 cut(s) 716, 877, 970, 1114
BsmAI GTCTC 3 cut(s) 295, 700, 1940
BsmFI GGGAC 4 cut(s) 974, 1258, 1893, 1933
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 4 cut(s) 34, 796, 1641, 2056
Bso31I GGTCTC 1 cut(s) 700
BsoBI CYCGRG 5 cut(s) 351, 1621, 1988, 2186, 2193
Bsp119I TTCGAA 1 cut(s) 409
Bsp1286I GDGCHC 4 cut(s) 387, 1030, 1959, 2193
Bsp1407I TGTACA 1 cut(s) 1888
Bsp143I GATC 7 cut(s) 331, 901, 933, 991, 1201, 1746, 2179
Bsp1720I GCTNAGC 1 cut(s) 381
BspACI CCGC 9 cut(s) 26, 38, 267, 282, 758, 1030, 1046, 1301, 1449
BspANI GGCC 4 cut(s) 34, 796, 1641, 2056
BspCNI CTCAG 5 cut(s) 373, 427, 790, 1763, 2085
BspFNI CGCG 1 cut(s) 269
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 3 cut(s) 621, 756, 962
BspPI GGATC 2 cut(s) 928, 1754
BspT104I TTCGAA 1 cut(s) 409
BspTI CTTAAG 1 cut(s) 1829
BspTNI GGTCTC 1 cut(s) 700
BsrBI CCGCTC 1 cut(s) 1451
BsrDI GCAATG 4 cut(s) 35, 81, 866, 1039
BsrFI RCCGGY 1 cut(s) 634
BsrGI TGTACA 1 cut(s) 1888
BsrI ACTGG 4 cut(s) 565, 797, 1507, 1828
BssAI RCCGGY 1 cut(s) 634
BssECI CCNNGG 8 cut(s) 246, 606, 1894, 1911, 1988, 1989, 2192, 2193
BssMI GATC 7 cut(s) 331, 901, 933, 991, 1201, 1746, 2179
BssNAI GTATAC 1 cut(s) 615
BssSI CACGAG 3 cut(s) 292, 1473, 2024
BssT1I CCWWGG 1 cut(s) 1894
Bst1107I GTATAC 1 cut(s) 615
Bst2BI CACGAG 3 cut(s) 292, 1473, 2024
Bst4CI ACNGT 6 cut(s) 247, 619, 1776, 1915, 2129, 2202
Bst6I CTCTTC 2 cut(s) 936, 1102
BstAFI CTTAAG 1 cut(s) 1829
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstAUI TGTACA 1 cut(s) 1888
BstBI TTCGAA 1 cut(s) 409
BstC8I GCNNGC 5 cut(s) 36, 954, 1030, 1080, 1985
BstDEI CTNAG 6 cut(s) 381, 414, 798, 1198, 1750, 2072
BstDSI CCRYGG 3 cut(s) 246, 606, 1911
BstF5I GGATG 6 cut(s) 46, 552, 601, 1372, 1721, 1852
BstFNI CGCG 1 cut(s) 269
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 7 cut(s) 334, 904, 936, 994, 1204, 1749, 2182
BstMAI GTCTC 3 cut(s) 295, 700, 1940
BstMBI GATC 7 cut(s) 331, 901, 933, 991, 1201, 1746, 2179
BstMWI GCNNNNNNNGC 3 cut(s) 1016, 1025, 1088
BstNSI RCATGY 6 cut(s) 437, 869, 929, 1082, 1987, 2215
BstSCI CCNGG 4 cut(s) 1987, 1988, 2192, 2193
BstSFI CTRYAG 3 cut(s) 572, 912, 1296
BstSLI GKGCMC 1 cut(s) 1030
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 3 cut(s) 364, 916, 1465
BstV2I GAAGAC 2 cut(s) 1220, 1960
BstX2I RGATCY 2 cut(s) 1201, 1746
BstXI CCANNNNNNTGG 1 cut(s) 653
BstYI RGATCY 2 cut(s) 1201, 1746
BstZ17I GTATAC 1 cut(s) 615
BsuRI GGCC 4 cut(s) 34, 796, 1641, 2056
BtgI CCRYGG 3 cut(s) 246, 606, 1911
BtgZI GCGATG 1 cut(s) 1713
BtrI CACGTC 1 cut(s) 1978
BtsCI GGATG 6 cut(s) 46, 552, 601, 1372, 1721, 1852
BtsIMutI CAGTG 3 cut(s) 1821, 2080, 2082
Cac8I GCNNGC 5 cut(s) 36, 954, 1030, 1080, 1985
CciI TCATGA 1 cut(s) 451
CfoI GCGC 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 634
Cfr13I GGNCC 2 cut(s) 961, 1276
Cfr9I CCCGGG 2 cut(s) 1988, 2193
Csp6I GTAC 5 cut(s) 161, 657, 1889, 2198, 2203
CspAI ACCGGT 1 cut(s) 634
CviQI GTAC 5 cut(s) 161, 657, 1889, 2198, 2203
DdeI CTNAG 6 cut(s) 381, 414, 798, 1198, 1750, 2072
DpnI GATC 7 cut(s) 333, 903, 935, 993, 1203, 1748, 2181
DpnII GATC 7 cut(s) 331, 901, 933, 991, 1201, 1746, 2179
DraI TTTAAA 1 cut(s) 489
DraIII CACNNNGTG 2 cut(s) 1963, 2075
DrdI GACNNNNNNGTC 1 cut(s) 1490
DseDI GACNNNNNNGTC 1 cut(s) 1490
EaeI YGGCCR 2 cut(s) 32, 2054
Eam1104I CTCTTC 2 cut(s) 936, 1102
EarI CTCTTC 2 cut(s) 936, 1102
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 1894
Eco24I GRGCYC 2 cut(s) 387, 2193
Eco31I GGTCTC 1 cut(s) 700
Eco47I GGWCC 2 cut(s) 961, 1276
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 828
Eco88I CYCGRG 5 cut(s) 351, 1621, 1988, 2186, 2193
EcoICRI GAGCTC 1 cut(s) 385
EcoO109I RGGNCCY 2 cut(s) 961, 1276
EcoT14I CCWWGG 1 cut(s) 1894
EcoT22I ATGCAT 2 cut(s) 1080, 2111
EcoT38I GRGCYC 2 cut(s) 387, 2193
ErhI CCWWGG 1 cut(s) 1894
FaqI GGGAC 4 cut(s) 974, 1258, 1893, 1933
FauI CCCGC 1 cut(s) 1037
FbaI TGATCA 1 cut(s) 901
FblI GTMKAC 1 cut(s) 614
Fnu4HI GCNGC 3 cut(s) 378, 930, 1479
FokI GGATG 6 cut(s) 53, 559, 588, 1379, 1728, 1859
FriOI GRGCYC 2 cut(s) 387, 2193
Fsp4HI GCNGC 3 cut(s) 378, 930, 1479
GlaI GCGC 1 cut(s) 252
GluI GCNGC 3 cut(s) 378, 930, 1479
GsuI CTGGAG 1 cut(s) 1563
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 4 cut(s) 34, 796, 1641, 2056
HapII CCGG 4 cut(s) 426, 635, 1989, 2194
HhaI GCGC 1 cut(s) 253
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HincII GTYRAC 2 cut(s) 279, 447
HindII GTYRAC 2 cut(s) 279, 447
HinfI GANTC 9 cut(s) 103, 349, 406, 448, 944, 1507, 1538, 1666, 1766
HpaI GTTAAC 1 cut(s) 279
HpaII CCGG 4 cut(s) 426, 635, 1989, 2194
HphI GGTGA 4 cut(s) 649, 1450, 1882, 1927
Hpy166II GTNNAC 4 cut(s) 279, 447, 615, 1891
Hpy188I TCNGA 9 cut(s) 73, 783, 847, 1287, 1468, 1671, 1753, 1940, 2179
Hpy8I GTNNAC 4 cut(s) 279, 447, 615, 1891
Hpy99I CGWCG 2 cut(s) 899, 1615
HpyAV CCTTC 6 cut(s) 265, 423, 852, 974, 1522, 1870
HpyCH4III ACNGT 6 cut(s) 247, 619, 1776, 1915, 2129, 2202
HpyCH4IV ACGT 2 cut(s) 1756, 1977
HpyF10VI GCNNNNNNNGC 3 cut(s) 1016, 1025, 1088
HpyF3I CTNAG 6 cut(s) 381, 414, 798, 1198, 1750, 2072
HpySE526I ACGT 2 cut(s) 1756, 1977
HspAI GCGC 1 cut(s) 251
Ksp22I TGATCA 1 cut(s) 901
KspAI GTTAAC 1 cut(s) 279
Kzo9I GATC 7 cut(s) 331, 901, 933, 991, 1201, 1746, 2179
LmnI GCTCC 4 cut(s) 197, 355, 973, 1544
Lsp1109I GCAGC 3 cut(s) 364, 916, 1465
LweI GCATC 6 cut(s) 300, 537, 825, 939, 1065, 1706
MaeII ACGT 2 cut(s) 1756, 1977
MaeIII GTNAC 7 cut(s) 1166, 1444, 1456, 1679, 1870, 1906, 2078
MalI GATC 7 cut(s) 333, 903, 935, 993, 1203, 1748, 2181
MbiI CCGCTC 1 cut(s) 1451
MboI GATC 7 cut(s) 331, 901, 933, 991, 1201, 1746, 2179
MfeI CAATTG 1 cut(s) 13
MflI RGATCY 2 cut(s) 1201, 1746
MhlI GDGCHC 4 cut(s) 387, 1030, 1959, 2193
MlsI TGGCCA 1 cut(s) 34
MluNI TGGCCA 1 cut(s) 34
MlyI GAGTC 5 cut(s) 343, 442, 953, 1516, 1532
MmeI TCCRAC 1 cut(s) 858
Mox20I TGGCCA 1 cut(s) 34
Mph1103I ATGCAT 2 cut(s) 1080, 2111
MscI TGGCCA 1 cut(s) 34
MslI CAYNNNNRTG 2 cut(s) 870, 1976
Msp20I TGGCCA 1 cut(s) 34
MspA1I CMGCKG 2 cut(s) 38, 284
MspCI CTTAAG 1 cut(s) 1829
MspI CCGG 4 cut(s) 426, 635, 1989, 2194
MspR9I CCNGG 4 cut(s) 1989, 1990, 2194, 2195
MunI CAATTG 1 cut(s) 13
Mva1269I GAATGC 1 cut(s) 235
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 3 cut(s) 1016, 1025, 1088
NciI CCSGG 4 cut(s) 1989, 1990, 2194, 2195
NdeII GATC 7 cut(s) 331, 901, 933, 991, 1201, 1746, 2179
NlaIV GGNNCC 3 cut(s) 621, 756, 962
NmuCI GTSAC 3 cut(s) 1456, 1870, 1906
NsiI ATGCAT 2 cut(s) 1080, 2111
NspI RCATGY 6 cut(s) 437, 869, 929, 1082, 1987, 2215
NspV TTCGAA 1 cut(s) 409
PaeI GCATGC 2 cut(s) 1082, 1987
PaeR7I CTCGAG 1 cut(s) 2186
PagI TCATGA 1 cut(s) 451
PcsI WCGNNNNNNNCGW 2 cut(s) 891, 1784
PctI GAATGC 1 cut(s) 235
PfeI GAWTC 4 cut(s) 103, 406, 1666, 1766
PflMI CCANNNNNTGG 1 cut(s) 877
PinAI ACCGGT 1 cut(s) 634
PkrI GCNGC 3 cut(s) 379, 931, 1480
PleI GAGTC 5 cut(s) 343, 442, 952, 1515, 1532
PpsI GAGTC 5 cut(s) 343, 442, 952, 1515, 1532
PpuMI RGGWCCY 2 cut(s) 961, 1276
PshBI ATTAAT 1 cut(s) 786
Psp124BI GAGCTC 1 cut(s) 387
Psp1406I AACGTT 1 cut(s) 1756
Psp5II RGGWCCY 2 cut(s) 961, 1276
PspN4I GGNNCC 3 cut(s) 621, 756, 962
PspPI GGNCC 2 cut(s) 961, 1276
PspPPI RGGWCCY 2 cut(s) 961, 1276
PsuI RGATCY 2 cut(s) 1201, 1746
RsaI GTAC 5 cut(s) 162, 658, 1890, 2199, 2204
RsaNI GTAC 5 cut(s) 161, 657, 1889, 2198, 2203
RseI CAYNNNNRTG 2 cut(s) 870, 1976
SacI GAGCTC 1 cut(s) 387
SatI GCNGC 3 cut(s) 378, 930, 1479
Sau3AI GATC 7 cut(s) 331, 901, 933, 991, 1201, 1746, 2179
Sau96I GGNCC 2 cut(s) 961, 1276
SchI GAGTC 5 cut(s) 343, 442, 953, 1516, 1532
ScrFI CCNGG 4 cut(s) 1989, 1990, 2194, 2195
SduI GDGCHC 4 cut(s) 387, 1030, 1959, 2193
SfaNI GCATC 6 cut(s) 300, 537, 825, 939, 1065, 1706
SfcI CTRYAG 3 cut(s) 572, 912, 1296
Sfr274I CTCGAG 1 cut(s) 2186
SfuI TTCGAA 1 cut(s) 409
SinI GGWCC 2 cut(s) 961, 1276
SlaI CTCGAG 1 cut(s) 2186
SmaI CCCGGG 2 cut(s) 1990, 2195
SmiMI CAYNNNNRTG 2 cut(s) 870, 1976
SmlI CTYRAG 5 cut(s) 110, 1519, 1560, 1829, 2186
SmoI CTYRAG 5 cut(s) 110, 1519, 1560, 1829, 2186
SpeI ACTAGT 1 cut(s) 659
SphI GCATGC 2 cut(s) 1082, 1987
SsiI CCGC 9 cut(s) 26, 38, 267, 282, 758, 1030, 1046, 1301, 1449
SspI AATATT 1 cut(s) 1392
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 4 cut(s) 1987, 1988, 2192, 2193
StyI CCWWGG 1 cut(s) 1894
TaaI ACNGT 6 cut(s) 247, 619, 1776, 1915, 2129, 2202
TaiI ACGT 2 cut(s) 1759, 1980
TaqI TCGA 7 cut(s) 101, 239, 387, 409, 947, 1995, 2187
TatI WGTACW 1 cut(s) 1888
TfiI GAWTC 4 cut(s) 103, 406, 1666, 1766
TscAI CASTG 3 cut(s) 1828, 2080, 2089
TseFI GTSAC 3 cut(s) 1456, 1870, 1906
TseI GCWGC 3 cut(s) 377, 929, 1478
Tsp45I GTSAC 3 cut(s) 1456, 1870, 1906
TspDTI ATGAA 9 cut(s) 57, 68, 70, 308, 1067, 1074, 1202, 1247, 1960
TspGWI ACGGA 5 cut(s) 138, 1013, 1130, 1432, 2006
TspMI CCCGGG 2 cut(s) 1988, 2193
TspRI CASTG 3 cut(s) 1828, 2080, 2089
Van91I CCANNNNNTGG 1 cut(s) 877
Vha464I CTTAAG 1 cut(s) 1829
VpaK11BI GGWCC 2 cut(s) 961, 1276
VspI ATTAAT 1 cut(s) 786
XapI RAATTY 5 cut(s) 1352, 1418, 1586, 1816, 2030
XbaI TCTAGA 1 cut(s) 2122
XceI RCATGY 6 cut(s) 437, 869, 929, 1082, 1987, 2215
XhoI CTCGAG 1 cut(s) 2186
XmaI CCCGGG 2 cut(s) 1988, 2193
XmiI GTMKAC 1 cut(s) 614
Zsp2I ATGCAT 2 cut(s) 1080, 2111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.