MD15G1275900.v1.1

May act early in the ethylene signal transduction pathway, possibly as an ethylene receptor, or as a regulator of the pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003094
Physical Location & Seq
Forward (+)
24226826 .. 24232215
5390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1275900.v1.1.491

Sequence Viewer

Length: 2544 bp
ATGATATCAAAACGCAGCGTTTTGAATCTGCGTGACGGTGAATTATATCAATGCAATGGATTATGGGTATCTCCATCCCCAATTTTCAAAACCCTAGTCAGCCCGTCGTCTCCTCTCCCACCCTCTCTTCTCCTCGGCTGGATCGTCGCACTGTGTTCGCTAGCGATATCAGTTCGCCGGCGCCGACTTGACCTGCCGCCGCGGACGGCCGGGGCTTTCTGCGACAGTCAATCGTCAGTCCACCCCCTCGCTTTCTGTGCTCATTTCTCACTTGCATGTAACAGACCAAGTAATATCCGTTTAAGTGGCTCAATCATCATGTTGGCATGCAACTGTATTGAGCCACAATGGCCGGCTGATGAATTGCTGATGAAATATCAGTACATTTCAGATTTCTTTATTGCGCTTGCTTATTTCTCCATCCCGCTAGAGTTGATCTACTTTGTCAAGAAGTCTGCAGTGTTTCCATACAGATGGGTCCTCGTACAGTTTGGTGCTTTCATTGTACTGTGTGGGGCGACTCATCTTATCAACTTATGGACGTTCAGCATACATTCAAGAACTGTGGCCATGGTAATGACCACGGCAAAGGTTTTAACTGCGGTGGTGTCATGCGCAACTGCCCTTATGCTTGTACATATTATACCTGATTTATTGAGTGTTAAAACTAGAGAACTATTCTTGAAGAACAAGGCGGCGGAGCTCGATAGAGAAATGGGCCTTATCCGTACACAGGAGGAAACAGGTCGACATGTCAGGATGTTGACCCATGAAATCAGAAGCACTCTTGATAGACATACTATACTTAAAACTACACTTGTCGAGTTAGGTAGAACTTTGGCATTGGAAGAGTGTGCCTTGTGGATGCCAACGCGTACTGGATTAGAGCTTCAACTATCCTATACACTTCGTCAGCAGAATCCAGTAGGATATACTGTACCCATTCATCTTCCTGTCATCAATCAAGTATTTAGTAGCAACCGTGCAGTAAAAATATCACCAAATTCTCCAGTGGCAAAACTGAGGCAACTTGCAGGAAGGCACATTCCTGGGGAAGTTGTTGCAGTTCGTGTGCCCCTTTTACATCTCTCTAACTTCCAAATTAATGACTGGCCTGAGCTCTCAACAAAACGCTATGCTTTGATGGTCTTGATGCTTCCCTCAGATAGTGCAAGGCAATGGCACGTTCACGAGTTAGAATTGGTAGAAGTGGTTGCTGATCAGGTTGCGGTTGCTCTGTCACATGCTGCAATATTAGAAGAGTCAATGAGGGCAAGGGATCTCCTTATGGAGCAGAATATTGCGCTTGATTTAGCCAGGAGAGAAGCGGAGACTGCAATTCGTGCTCGCAATGATTTCTTGGCTGTTATGAACCATGAGATGAGAACCCCCATGCATGCAATTATTGCTCTTTCTTCTTTGCTGCAGGAAACTGAGCTCACAGCTGAGCAGCGTCTCATGGTTGAAACGATATTAAGGAGCAGCAACCTATTGGCTACCCTCATAAATGATGTTTTGGATCTTTCAAGGCTTGAAGATGGCAGCCTTCAACTTGAAATAGCAACTTTTAACCTCCATTCCGTATTTAGAGAGGTTCATAATATGATTAAGCCTGTTGCATCTATCAAAAGGTTGTCAGTTACATTGAATATAGCTGCAGATTTGCCAATGTATGCCATAGGTGATGAAAAGCGTCTTATGCAAACTATTCTAAATGTTGTTGGGAATGCTGTGAAGTTCTCAAAAGAAGGGAGCATCTCAATCACAGCTTTTGTTGCAAAATCAGAATCTTTACGAGATTTCCGGGCTCCTGATTTCTTTCCAGTGCAAAGTGATAATCACTTTTATCTGCGCGTACAGGTCAAAGATTCAGGATCTGGAATCAATCCTCAGGATATCCCAAAACTATTTACCAAATTTGCACAAACTCAGGCTTTAGCTACTCGAAATTCTGGTGGTAGTGGACTTGGCCTGGCAATTTGTAAGAGATTTGTAAATCTTATGGAAGGGCATATTTGGATTGAAAGTGAAGGTCTTGGCAAGGGATGCACGGCCACTTTCATTGTGAAACTCGGATTTCCAGAGCGTTCGAATGAGTCTAAGCTGCCTTTTGCACCCAAGCTGCAAGCAAATCATGTTCAAACTAACTTTCCAGGGTTGAAAGTCCTTGTCATGGATGATAATGGGGTCAGCAGGTCAGTGACAAAGGGACTCCTTGCGCACCTAGGATGTGATGTAACAGCTGTAAGCTTAATCGATGAATTATTGCATGTTATTTCACAAGAGCACAAGGTGGTGTTCATGGATGTGAGCATGCCAGGAATCGATGGTTATGAACTTGCTGTCCGTATACATGAAAAGTTCACAAAACGTCATGAGCGGCCGGTATTAGTGGCACTTACCGGAAGCATAGACAAGATTACAAAGGAGAACTGCATGAGGGTTGGTGTGGATGGTGTTATACTAAAACCGGTTTCAGTCGATAAAATGAGGAGTGTTTTATCAGAATTATTAGAGCATCGAGTCCTATTCGAGGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009308 GO:0009408 GO:0009593 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009651 GO:0009690 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009733 GO:0009737 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010087 GO:0010104 GO:0010105 GO:0010119 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016999 GO:0017000 GO:0017144 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0023051 GO:0023052 GO:0023057 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0033993 GO:0034754 GO:0035556 GO:0036211 GO:0038023 GO:0038199 GO:0042221 GO:0042445 GO:0042545 GO:0042562 GO:0042742 GO:0042743 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046483 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048856 GO:0050665 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051239 GO:0051301 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0051740 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0072328 GO:0072593 GO:0097305 GO:0098542 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1903409 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
GAF HATPase_c HisKA Response_reg

Protein Analysis

848

Amino Acids

94.52

Weight (kDa)

8.62

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETR1_N PF25487 123 - 221 2.5e-39 Ethylene receptor 1-like, N-terminal domain
GAF PF01590 264 - 413 1.5e-13 GAF domain
HisKA PF00512 449 - 514 9.4e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 561 - 691 9.7e-25 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 722 - 833 3.5e-16 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 616, 2220
Acc36I ACCTGC 2 cut(s) 201, 2184
AccB1I GGYRCC 1 cut(s) 180
AccBSI CCGCTC 1 cut(s) 2380
AccI GTMKAC 2 cut(s) 748, 2350
AccII CGCG 3 cut(s) 202, 874, 1854
AclWI GGATC 4 cut(s) 149, 1287, 1527, 1882
AcoI YGGCCR 5 cut(s) 207, 350, 567, 2052, 2381
AcsI RAATTY 3 cut(s) 1003, 1916, 1948
AcyI GRCGYC 1 cut(s) 181
AdeI CACNNNGTG 1 cut(s) 2293
AfaI GTAC 8 cut(s) 383, 486, 507, 636, 730, 877, 939, 1857
AfiI CCNNNNNNNGG 3 cut(s) 733, 2173, 2533
AflIII ACRYGT 2 cut(s) 751, 872
AgeI ACCGGT 1 cut(s) 2470
AjnI CCWGG 5 cut(s) 1048, 1316, 1971, 2152, 2317
AloI GAACNNNNNNTCC 2 cut(s) 2328, 2360
Alw21I GWGCWC 6 cut(s) 262, 705, 1122, 1348, 1440, 2289
Alw26I GTCTC 3 cut(s) 114, 1325, 1460
AlwI GGATC 4 cut(s) 149, 1287, 1527, 1882
AlwNI CAGNNNCTG 1 cut(s) 1877
AoxI GGCC 9 cut(s) 207, 350, 567, 718, 1112, 1969, 2052, 2381, 2535
ApeKI GCWGC 9 cut(s) 15, 1247, 1423, 1450, 1482, 1542, 1655, 2104, 2122
ApoI RAATTY 3 cut(s) 1003, 1916, 1948
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AseI ATTAAT 1 cut(s) 1104
AsiGI ACCGGT 1 cut(s) 2470
AspA2I CCTAGG 1 cut(s) 2224
AspLEI GCGC 6 cut(s) 183, 406, 617, 1306, 1854, 2221
AspS9I GGNCC 2 cut(s) 478, 718
AsuC2I CCSGG 2 cut(s) 211, 1805
AsuHPI GGTGA 3 cut(s) 50, 990, 1694
AsuII TTCGAA 1 cut(s) 2090
AsuNHI GCTAGC 1 cut(s) 160
AvaII GGWCC 1 cut(s) 478
AvrII CCTAGG 1 cut(s) 2224
AxyI CCTNAGG 1 cut(s) 1890
BaeGI GKGCMC 1 cut(s) 1077
BalI TGGCCA 1 cut(s) 569
BanI GGYRCC 1 cut(s) 180
BanII GRGCYC 4 cut(s) 705, 1122, 1440, 1810
BauI CACGAG 1 cut(s) 1190
Bbv12I GWGCWC 6 cut(s) 262, 705, 1122, 1348, 1440, 2289
BbvI GCAGC 9 cut(s) 27, 1234, 1410, 1462, 1494, 1554, 1642, 2091, 2109
BccI CCATC 7 cut(s) 82, 428, 468, 1138, 1532, 2321, 2447
BceAI ACGGC 3 cut(s) 222, 600, 2067
BcgI CGANNNNNNTGC 2 cut(s) 138, 172
BciT130I CCWGG 5 cut(s) 1050, 1318, 1973, 2154, 2319
BclI TGATCA 1 cut(s) 1219
BcnI CCSGG 2 cut(s) 211, 1805
BcoDI GTCTC 3 cut(s) 114, 1325, 1460
BfaI CTAG 5 cut(s) 95, 161, 428, 669, 2225
BfmI CTRYAG 3 cut(s) 456, 1424, 1656
BfoI RGCGCY 1 cut(s) 184
BfuAI ACCTGC 2 cut(s) 201, 2184
BglI GCCNNNNNGGC 1 cut(s) 349
BlnI CCTAGG 1 cut(s) 2224
BlpI GCTNAGC 1 cut(s) 1446
Bme1390I CCNGG 7 cut(s) 211, 1050, 1318, 1805, 1973, 2154, 2319
Bme18I GGWCC 1 cut(s) 478
BmgT120I GGNCC 2 cut(s) 478, 718
BmiI GGNNCC 3 cut(s) 182, 479, 1809
BmrFI CCNGG 7 cut(s) 211, 1050, 1318, 1805, 1973, 2154, 2319
BmsI GCATC 6 cut(s) 855, 1143, 1628, 1764, 2036, 2527
BmtI GCTAGC 1 cut(s) 164
BpmI CTGGAG 1 cut(s) 993
Bpu10I CCTNAGC 1 cut(s) 1116
Bpu1102I GCTNAGC 1 cut(s) 1446
Bpu14I TTCGAA 1 cut(s) 2090
BpuMI CCSGG 2 cut(s) 211, 1805
Bsa29I ATCGAT 2 cut(s) 2256, 2325
BsaHI GRCGYC 1 cut(s) 181
BsaJI CCNNGG 8 cut(s) 133, 200, 210, 570, 582, 1049, 2153, 2224
BsaWI WCCGGW 2 cut(s) 2402, 2470
Bsc4I CCNNNNNNNGG 3 cut(s) 733, 2173, 2533
Bse118I RCCGGY 4 cut(s) 177, 352, 2383, 2470
Bse1I ACTGG 5 cut(s) 883, 923, 1010, 1115, 1823
Bse21I CCTNAGG 1 cut(s) 1890
Bse3DI GCAATG 3 cut(s) 61, 1184, 1357
BseBI CCWGG 5 cut(s) 1050, 1318, 1973, 2154, 2319
BseCI ATCGAT 2 cut(s) 2256, 2325
BseDI CCNNGG 8 cut(s) 133, 200, 210, 570, 582, 1049, 2153, 2224
BseGI GGATG 9 cut(s) 74, 420, 765, 870, 2051, 2182, 2234, 2311, 2458
BseLI CCNNNNNNNGG 3 cut(s) 733, 2173, 2533
BseMI GCAATG 3 cut(s) 61, 1184, 1357
BseMII CTCAG 7 cut(s) 1013, 1107, 1176, 1425, 1437, 1904, 1943
BseNI ACTGG 5 cut(s) 883, 923, 1010, 1115, 1823
BseRI GAGGAG 3 cut(s) 102, 122, 2506
BseSI GKGCMC 1 cut(s) 1077
BseX3I CGGCCG 2 cut(s) 207, 2381
BseXI GCAGC 9 cut(s) 27, 1234, 1410, 1462, 1494, 1554, 1642, 2091, 2109
BsgI GTGCAG 1 cut(s) 1005
Bsh1236I CGCG 3 cut(s) 202, 874, 1854
Bsh1285I CGRYCG 2 cut(s) 210, 2384
BshFI GGCC 9 cut(s) 209, 352, 569, 720, 1114, 1971, 2054, 2383, 2537
BshNI GGYRCC 1 cut(s) 180
BshTI ACCGGT 1 cut(s) 2470
BshVI ATCGAT 2 cut(s) 2256, 2325
BsiEI CGRYCG 2 cut(s) 210, 2384
BsiHKAI GWGCWC 6 cut(s) 262, 705, 1122, 1348, 1440, 2289
BsiSI CCGG 7 cut(s) 178, 210, 353, 1804, 2384, 2403, 2471
BslFI GGGAC 1 cut(s) 2223
BslI CCNNNNNNNGG 3 cut(s) 733, 2173, 2533
BsmAI GTCTC 3 cut(s) 114, 1325, 1460
BsmBI CGTCTC 2 cut(s) 114, 1460
BsmFI GGGAC 1 cut(s) 2223
BsmI GAATGC 1 cut(s) 1732
BsnI GGCC 9 cut(s) 209, 352, 569, 720, 1114, 1971, 2054, 2383, 2537
Bsp119I TTCGAA 1 cut(s) 2090
Bsp1286I GDGCHC 8 cut(s) 262, 705, 1077, 1122, 1348, 1440, 1810, 2289
Bsp1407I TGTACA 1 cut(s) 634
Bsp143I GATC 6 cut(s) 141, 435, 1219, 1279, 1519, 1874
Bsp1720I GCTNAGC 1 cut(s) 1446
Bsp19I CCATGG 1 cut(s) 570
BspANI GGCC 9 cut(s) 209, 352, 569, 720, 1114, 1971, 2054, 2383, 2537
BspCNI CTCAG 7 cut(s) 1014, 1108, 1175, 1426, 1438, 1903, 1942
BspDI ATCGAT 2 cut(s) 2256, 2325
BspFNI CGCG 3 cut(s) 202, 874, 1854
BspHI TCATGA 1 cut(s) 2374
BspLI GGNNCC 3 cut(s) 182, 479, 1809
BspMAI CTGCAG 3 cut(s) 460, 1428, 1660
BspMI ACCTGC 2 cut(s) 201, 2184
BspOI GCTAGC 1 cut(s) 164
BspPI GGATC 4 cut(s) 149, 1287, 1527, 1882
BspT104I TTCGAA 1 cut(s) 2090
BspT107I GGYRCC 1 cut(s) 180
BsrBI CCGCTC 1 cut(s) 2380
BsrDI GCAATG 3 cut(s) 61, 1184, 1357
BsrFI RCCGGY 4 cut(s) 177, 352, 2383, 2470
BsrGI TGTACA 1 cut(s) 634
BsrI ACTGG 5 cut(s) 883, 923, 1010, 1115, 1823
BssAI RCCGGY 4 cut(s) 177, 352, 2383, 2470
BssECI CCNNGG 8 cut(s) 133, 200, 210, 570, 582, 1049, 2153, 2224
BssMI GATC 6 cut(s) 141, 435, 1219, 1279, 1519, 1874
BssNAI GTATAC 1 cut(s) 2351
BssNI GRCGYC 1 cut(s) 181
BssSI CACGAG 1 cut(s) 1190
BssT1I CCWWGG 2 cut(s) 570, 2224
Bst1107I GTATAC 1 cut(s) 2351
Bst2BI CACGAG 1 cut(s) 1190
Bst2UI CCWGG 5 cut(s) 1050, 1318, 1973, 2154, 2319
Bst4CI ACNGT 9 cut(s) 38, 153, 227, 335, 489, 510, 565, 937, 983
Bst6I CTCTTC 3 cut(s) 132, 843, 1254
BstACI GRCGYC 1 cut(s) 181
BstAPI GCANNNNNTGC 3 cut(s) 1343, 1406, 2046
BstAUI TGTACA 1 cut(s) 634
BstBI TTCGAA 1 cut(s) 2090
BstC8I GCNNGC 9 cut(s) 162, 179, 328, 354, 408, 1348, 1398, 2127, 2315
BstDEI CTNAG 8 cut(s) 1022, 1116, 1162, 1434, 1446, 1890, 1929, 2100
BstDSI CCRYGG 3 cut(s) 200, 570, 582
BstENI CCTNNNNNAGG 1 cut(s) 2531
BstF5I GGATG 9 cut(s) 74, 420, 765, 870, 2051, 2182, 2234, 2311, 2458
BstFNI CGCG 3 cut(s) 202, 874, 1854
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 6 cut(s) 183, 406, 617, 1306, 1854, 2221
BstKTI GATC 6 cut(s) 144, 438, 1222, 1282, 1522, 1877
BstMAI GTCTC 3 cut(s) 114, 1325, 1460
BstMBI GATC 6 cut(s) 141, 435, 1219, 1279, 1519, 1874
BstMCI CGRYCG 2 cut(s) 210, 2384
BstMWI GCNNNNNNNGC 8 cut(s) 257, 349, 1334, 1343, 1406, 1699, 1775, 2046
BstNI CCWGG 5 cut(s) 1050, 1318, 1973, 2154, 2319
BstNSI RCATGY 7 cut(s) 279, 330, 755, 1247, 1400, 2273, 2317
BstSCI CCNGG 7 cut(s) 209, 1048, 1316, 1803, 1971, 2152, 2317
BstSFI CTRYAG 3 cut(s) 456, 1424, 1656
BstSLI GKGCMC 1 cut(s) 1077
BstUI CGCG 3 cut(s) 202, 874, 1854
BstV1I GCAGC 9 cut(s) 27, 1234, 1410, 1462, 1494, 1554, 1642, 2091, 2109
BstX2I RGATCY 3 cut(s) 1279, 1519, 1874
BstXI CCANNNNNNTGG 1 cut(s) 474
BstYI RGATCY 3 cut(s) 1279, 1519, 1874
BstZ17I GTATAC 1 cut(s) 2351
BstZI CGGCCG 2 cut(s) 207, 2381
Bsu15I ATCGAT 2 cut(s) 2256, 2325
Bsu36I CCTNAGG 1 cut(s) 1890
BsuRI GGCC 9 cut(s) 209, 352, 569, 720, 1114, 1971, 2054, 2383, 2537
BsuTUI ATCGAT 2 cut(s) 2256, 2325
BtgI CCRYGG 3 cut(s) 200, 570, 582
BtsCI GGATG 9 cut(s) 74, 420, 765, 870, 2051, 2182, 2234, 2311, 2458
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 5 cut(s) 149, 465, 1017, 1830, 2205
BveI ACCTGC 2 cut(s) 201, 2184
Cac8I GCNNGC 9 cut(s) 162, 179, 328, 354, 408, 1348, 1398, 2127, 2315
CaiI CAGNNNCTG 1 cut(s) 1877
CciI TCATGA 1 cut(s) 2374
CfoI GCGC 6 cut(s) 183, 406, 617, 1306, 1854, 2221
Cfr10I RCCGGY 4 cut(s) 177, 352, 2383, 2470
Cfr13I GGNCC 2 cut(s) 478, 718
Cfr42I CCGCGG 1 cut(s) 203
ClaI ATCGAT 2 cut(s) 2256, 2325
CseI GACGC 2 cut(s) 1442, 1682
Csp6I GTAC 8 cut(s) 382, 485, 506, 635, 729, 876, 938, 1856
CspAI ACCGGT 1 cut(s) 2470
CspCI CAANNNNNGTGG 4 cut(s) 546, 581, 2044, 2079
CviQI GTAC 8 cut(s) 382, 485, 506, 635, 729, 876, 938, 1856
DdeI CTNAG 8 cut(s) 1022, 1116, 1162, 1434, 1446, 1890, 1929, 2100
DinI GGCGCC 1 cut(s) 182
DpnI GATC 6 cut(s) 143, 437, 1221, 1281, 1521, 1876
DpnII GATC 6 cut(s) 141, 435, 1219, 1279, 1519, 1874
DraIII CACNNNGTG 1 cut(s) 2293
EaeI YGGCCR 5 cut(s) 207, 350, 567, 2052, 2381
EagI CGGCCG 2 cut(s) 207, 2381
Eam1104I CTCTTC 3 cut(s) 132, 843, 1254
EarI CTCTTC 3 cut(s) 132, 843, 1254
EciI GGCGGA 1 cut(s) 713
Ecl136II GAGCTC 3 cut(s) 703, 1120, 1438
EclXI CGGCCG 2 cut(s) 207, 2381
Eco130I CCWWGG 2 cut(s) 570, 2224
Eco24I GRGCYC 4 cut(s) 705, 1122, 1440, 1810
Eco32I GATATC 3 cut(s) 6, 168, 1897
Eco47I GGWCC 1 cut(s) 478
Eco52I CGGCCG 2 cut(s) 207, 2381
Eco53kI GAGCTC 3 cut(s) 703, 1120, 1438
Eco81I CCTNAGG 1 cut(s) 1890
EcoICRI GAGCTC 3 cut(s) 703, 1120, 1438
EcoNI CCTNNNNNAGG 1 cut(s) 2531
EcoO109I RGGNCCY 1 cut(s) 478
EcoRII CCWGG 5 cut(s) 1048, 1316, 1971, 2152, 2317
EcoRV GATATC 3 cut(s) 6, 168, 1897
EcoT14I CCWWGG 2 cut(s) 570, 2224
EcoT22I ATGCAT 1 cut(s) 1398
EcoT38I GRGCYC 4 cut(s) 705, 1122, 1440, 1810
EgeI GGCGCC 1 cut(s) 182
EheI GGCGCC 1 cut(s) 182
ErhI CCWWGG 2 cut(s) 570, 2224
Esp3I CGTCTC 2 cut(s) 114, 1460
FaqI GGGAC 1 cut(s) 2223
FauI CCCGC 1 cut(s) 432
FbaI TGATCA 1 cut(s) 1219
FblI GTMKAC 2 cut(s) 748, 2350
FokI GGATG 9 cut(s) 61, 407, 772, 877, 2058, 2189, 2241, 2318, 2465
FriOI GRGCYC 4 cut(s) 705, 1122, 1440, 1810
FspBI CTAG 5 cut(s) 95, 161, 428, 669, 2225
FspI TGCGCA 2 cut(s) 616, 2220
GlaI GCGC 6 cut(s) 182, 405, 616, 1305, 1853, 2220
GsuI CTGGAG 1 cut(s) 993
HaeII RGCGCY 1 cut(s) 184
HaeIII GGCC 9 cut(s) 209, 352, 569, 720, 1114, 1971, 2054, 2383, 2537
HapII CCGG 7 cut(s) 178, 210, 353, 1804, 2384, 2403, 2471
HgaI GACGC 2 cut(s) 1442, 1682
HhaI GCGC 6 cut(s) 183, 406, 617, 1306, 1854, 2221
Hin1I GRCGYC 1 cut(s) 181
Hin6I GCGC 6 cut(s) 181, 404, 615, 1304, 1852, 2219
HinP1I GCGC 6 cut(s) 181, 404, 615, 1304, 1852, 2219
HincII GTYRAC 2 cut(s) 749, 765
HindII GTYRAC 2 cut(s) 749, 765
HindIII AAGCTT 1 cut(s) 2248
HpaII CCGG 7 cut(s) 178, 210, 353, 1804, 2384, 2403, 2471
HphI GGTGA 3 cut(s) 50, 990, 1694
Hpy166II GTNNAC 8 cut(s) 241, 731, 749, 765, 1189, 1964, 2351, 2364
Hpy188I TCNGA 6 cut(s) 391, 779, 1165, 1786, 2075, 2506
Hpy8I GTNNAC 8 cut(s) 241, 731, 749, 765, 1189, 1964, 2351, 2364
Hpy99I CGWCG 2 cut(s) 109, 149
HpyAV CCTTC 5 cut(s) 1032, 1556, 1742, 2000, 2024
HpyCH4III ACNGT 9 cut(s) 38, 153, 227, 335, 489, 510, 565, 937, 983
HpyCH4IV ACGT 3 cut(s) 542, 1185, 2371
HpyF10VI GCNNNNNNNGC 8 cut(s) 257, 349, 1334, 1343, 1406, 1699, 1775, 2046
HpyF3I CTNAG 8 cut(s) 1022, 1116, 1162, 1434, 1446, 1890, 1929, 2100
HpySE526I ACGT 3 cut(s) 542, 1185, 2371
Hsp92I GRCGYC 1 cut(s) 181
HspAI GCGC 6 cut(s) 181, 404, 615, 1304, 1852, 2219
KasI GGCGCC 1 cut(s) 180
KroI GCCGGC 2 cut(s) 177, 352
KroNI GCCGGC 2 cut(s) 179, 354
Ksp22I TGATCA 1 cut(s) 1219
KspI CCGCGG 1 cut(s) 203
Kzo9I GATC 6 cut(s) 141, 435, 1219, 1279, 1519, 1874
LmnI GCTCC 5 cut(s) 700, 1291, 1479, 1752, 1813
Lsp1109I GCAGC 9 cut(s) 27, 1234, 1410, 1462, 1494, 1554, 1642, 2091, 2109
LweI GCATC 6 cut(s) 855, 1143, 1628, 1764, 2036, 2527
MaeI CTAG 5 cut(s) 95, 161, 428, 669, 2225
MaeII ACGT 3 cut(s) 542, 1185, 2371
MaeIII GTNAC 6 cut(s) 32, 278, 1239, 1639, 2200, 2236
MalI GATC 6 cut(s) 143, 437, 1221, 1281, 1521, 1876
MbiI CCGCTC 1 cut(s) 2380
MboI GATC 6 cut(s) 141, 435, 1219, 1279, 1519, 1874
MboII GAAGA 7 cut(s) 119, 697, 860, 941, 1271, 1407, 1547
MflI RGATCY 3 cut(s) 1279, 1519, 1874
MhlI GDGCHC 8 cut(s) 262, 705, 1077, 1122, 1348, 1440, 1810, 2289
MlsI TGGCCA 1 cut(s) 569
MluI ACGCGT 1 cut(s) 872
MluNI TGGCCA 1 cut(s) 569
Mly113I GGCGCC 1 cut(s) 181
MlyI GAGTC 5 cut(s) 514, 1271, 2105, 2205, 2532
Mox20I TGGCCA 1 cut(s) 569
Mph1103I ATGCAT 1 cut(s) 1398
MreI CGCCGGCG 1 cut(s) 177
MroNI GCCGGC 2 cut(s) 177, 352
MscI TGGCCA 1 cut(s) 569
MseI TTAA 9 cut(s) 302, 596, 663, 807, 1104, 1475, 1569, 1608, 2252
MslI CAYNNNNRTG 4 cut(s) 274, 472, 575, 2306
Msp20I TGGCCA 1 cut(s) 569
MspA1I CMGCKG 3 cut(s) 202, 1445, 2243
MspI CCGG 7 cut(s) 178, 210, 353, 1804, 2384, 2403, 2471
MspR9I CCNGG 7 cut(s) 211, 1050, 1318, 1805, 1973, 2154, 2319
Mva1269I GAATGC 1 cut(s) 1732
MvaI CCWGG 5 cut(s) 1050, 1318, 1973, 2154, 2319
MvnI CGCG 3 cut(s) 202, 874, 1854
MwoI GCNNNNNNNGC 8 cut(s) 257, 349, 1334, 1343, 1406, 1699, 1775, 2046
NaeI GCCGGC 2 cut(s) 179, 354
NarI GGCGCC 1 cut(s) 181
NciI CCSGG 2 cut(s) 211, 1805
NcoI CCATGG 1 cut(s) 570
NdeII GATC 6 cut(s) 141, 435, 1219, 1279, 1519, 1874
NgoMIV GCCGGC 2 cut(s) 177, 352
NheI GCTAGC 1 cut(s) 160
NlaIV GGNNCC 3 cut(s) 182, 479, 1809
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 3 cut(s) 32, 1239, 2200
NsbI TGCGCA 2 cut(s) 616, 2220
NsiI ATGCAT 1 cut(s) 1398
NspI RCATGY 7 cut(s) 279, 330, 755, 1247, 1400, 2273, 2317
NspV TTCGAA 1 cut(s) 2090
PaeI GCATGC 3 cut(s) 330, 1400, 2317
PagI TCATGA 1 cut(s) 2374
PciI ACATGT 1 cut(s) 751
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 181
PctI GAATGC 1 cut(s) 1732
PdiI GCCGGC 2 cut(s) 179, 354
PfeI GAWTC 6 cut(s) 25, 919, 1787, 1868, 1881, 2322
PinAI ACCGGT 1 cut(s) 2470
PleI GAGTC 5 cut(s) 514, 1270, 2104, 2205, 2531
PluTI GGCGCC 1 cut(s) 184
PpsI GAGTC 5 cut(s) 514, 1270, 2104, 2205, 2531
PpuMI RGGWCCY 1 cut(s) 478
PscI ACATGT 1 cut(s) 751
PshBI ATTAAT 1 cut(s) 1104
Psp124BI GAGCTC 3 cut(s) 705, 1122, 1440
Psp5II RGGWCCY 1 cut(s) 478
Psp6I CCWGG 5 cut(s) 1048, 1316, 1971, 2152, 2317
PspGI CCWGG 5 cut(s) 1048, 1316, 1971, 2152, 2317
PspN4I GGNNCC 3 cut(s) 182, 479, 1809
PspPI GGNCC 2 cut(s) 478, 718
PspPPI RGGWCCY 1 cut(s) 478
PstI CTGCAG 3 cut(s) 460, 1428, 1660
PstNI CAGNNNCTG 1 cut(s) 1877
PsuI RGATCY 3 cut(s) 1279, 1519, 1874
PvuII CAGCTG 2 cut(s) 1445, 2243
RsaI GTAC 8 cut(s) 383, 486, 507, 636, 730, 877, 939, 1857
RsaNI GTAC 8 cut(s) 382, 485, 506, 635, 729, 876, 938, 1856
RseI CAYNNNNRTG 4 cut(s) 274, 472, 575, 2306
SacI GAGCTC 3 cut(s) 705, 1122, 1440
SacII CCGCGG 1 cut(s) 203
SalI GTCGAC 1 cut(s) 747
SaqAI TTAA 9 cut(s) 302, 596, 663, 807, 1104, 1475, 1569, 1608, 2252
Sau3AI GATC 6 cut(s) 141, 435, 1219, 1279, 1519, 1874
Sau96I GGNCC 2 cut(s) 478, 718
SchI GAGTC 5 cut(s) 514, 1271, 2105, 2205, 2532
ScrFI CCNGG 7 cut(s) 211, 1050, 1318, 1805, 1973, 2154, 2319
SduI GDGCHC 8 cut(s) 262, 705, 1077, 1122, 1348, 1440, 1810, 2289
SfaNI GCATC 6 cut(s) 855, 1143, 1628, 1764, 2036, 2527
SfcI CTRYAG 3 cut(s) 456, 1424, 1656
SfoI GGCGCC 1 cut(s) 182
Sfr303I CCGCGG 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 2090
SgrAI CRCCGGYG 1 cut(s) 177
SgrBI CCGCGG 1 cut(s) 203
SinI GGWCC 1 cut(s) 478
SmiMI CAYNNNNRTG 4 cut(s) 274, 472, 575, 2306
SphI GCATGC 3 cut(s) 330, 1400, 2317
SspDI GGCGCC 1 cut(s) 180
SspI AATATT 2 cut(s) 1254, 1300
SspMI CTAG 5 cut(s) 95, 161, 428, 669, 2225
SstI GAGCTC 3 cut(s) 705, 1122, 1440
StyD4I CCNGG 7 cut(s) 209, 1048, 1316, 1803, 1971, 2152, 2317
StyI CCWWGG 2 cut(s) 570, 2224
TaaI ACNGT 9 cut(s) 38, 153, 227, 335, 489, 510, 565, 937, 983
TaiI ACGT 3 cut(s) 545, 1188, 2374
TatI WGTACW 3 cut(s) 381, 505, 634
TauI GCSGC 4 cut(s) 199, 202, 698, 2383
TfiI GAWTC 6 cut(s) 25, 919, 1787, 1868, 1881, 2322
Tru1I TTAA 9 cut(s) 302, 596, 663, 807, 1104, 1475, 1569, 1608, 2252
Tru9I TTAA 9 cut(s) 302, 596, 663, 807, 1104, 1475, 1569, 1608, 2252
TscAI CASTG 5 cut(s) 156, 465, 1017, 1830, 2205
TseFI GTSAC 3 cut(s) 32, 1239, 2200
TseI GCWGC 9 cut(s) 15, 1247, 1423, 1450, 1482, 1542, 1655, 2104, 2122
Tsp45I GTSAC 3 cut(s) 32, 1239, 2200
TspGWI ACGGA 4 cut(s) 287, 716, 1570, 2336
TspRI CASTG 5 cut(s) 156, 465, 1017, 1830, 2205
VpaK11BI GGWCC 1 cut(s) 478
VspI ATTAAT 1 cut(s) 1104
XagI CCTNNNNNAGG 1 cut(s) 2531
XapI RAATTY 3 cut(s) 1003, 1916, 1948
XceI RCATGY 7 cut(s) 279, 330, 755, 1247, 1400, 2273, 2317
XmaJI CCTAGG 1 cut(s) 2224
XmiI GTMKAC 2 cut(s) 748, 2350
XspI CTAG 5 cut(s) 95, 161, 428, 669, 2225
Zsp2I ATGCAT 1 cut(s) 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.