Rroxscaffold_3G00232470

May act early in the ethylene signal transduction pathway, possibly as an ethylene receptor, or as a regulator of the pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003094
Physical Location & Seq
Forward (+)
18103813 .. 18111828
8016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00232470.1

Sequence Viewer

Length: 2337 bp
ATGGCATTCGAGGTGGCGGGAGTGGTGATCAACATGGCGGCGGCGGCGGAGGCGACCAAGTTGGCAATAATTCTTCTAGGGTTTGTGTTGAATGACTGTCTTGGGCACGGGTGTGATCTTGGTTGGGAGTCCTTGCTTGGGTTTCCTATTCCTGGGCTTGGTGGGCCTGCCGGGATTCTTGTAGGTGTAGTTTATGAACTCCTAGAAACAAGTGGTATTCATTCAAGTGGCTCAATCATGATGGAGGTGTGCAACTGCATTGAGCCACAATGGCCGGCTGATGAATTGTTGATGAAGTATCAGTACATTTCAGATTTCTTTATTGCACTTGCTTATTTCTCCATCCCTCTGGAGTTAATCTACTTTGTCAAGAAATCTGCCGTGTTTCCATACAAATGGGTTCTTGTTCAGTTTGGTGCTTTCATTGTATTGTGTGGAGCGACACATCTTATTAACTTATGGACTTTTAGCATGCATTCAAGAACTGTGGCCATAGTAATGGCTACTGCCAAGGTTCTAACTGCTGCGGTGTCATGTGCTACTGCCCTCATGCTTGTACATATTATTCCTGATTTACTGAGTGTTAAAACTAGAGAGTTATTCTTGAAGAACAGGGCTGCAGAGCTCGACAGAGAAATGGGTCTGATTCGTACACAGGAAGAAACAGGTAGACATGTCAGGATGCTGACGCATGAGATCAGAAGCACTCTTGATCGACATACTATACTGAAAACCACACTTGTTGAATTAGGTAGAATTTTGACATTGGAAGAGTGTGCCTTGTGGATGCCAACACGCACTGGATTGGAGCTTCAACTATCCTATACTCTTCATCAGCAGAATCCAGTAGGATACACTGTACCCATCCATCTTCCTGTGATCAATCAAGTATTTAACAACAATGGTACAGTAAAAATATCACCAAATTCCCCAGTGGCCAGACTAAGGCCTATTGCTGGAAAACACATTCCTGGGGAGGTTGTTGCAATTCGTGTCCCACTCCTACATCTTTCTAACTTCCAAATTAATGACTGGCCAGAGCTTTCAACAAAACGCTATGCTTTGATGGTGTTGATGCTTCCCTCTGATAGTGCTAGGCAATGGCATGTCCATGAGTTAGAGCTGGTGGAGGTAGTTGCTGATCAGGTGGCAGTTGCTCTTTCACATGCTGCAATATTAGAAGAGTCAAGGAAGGCCAGGGATCTTCTTGTGGAGCAGAATATTGCACTTGATTTGGCCAGGAGGGACGCTGAAATTGCAATTCATGCTCGCAATGATTTCTTGGCTGTTATGAACCATGAGATGAGAACTCCCATGCATGCAATCATTGCCCTTTCTTCCTTGCTGCAAGAAACTGAACTGACACCTGAGCAGCGCCTGATGGTTGAAACAATACTAAAGAGCAGTAACCTTTTGGCTACCCTCATTAATGATGTATTAGATCTTTCAAGGCTTGAAGATGGCAGCCTTCAACTCGATATGGCAACTTTTAACCTCCATTCTGTATTCAGAGAGGTGCACAGTTTAGTTAAGCCTGTTGCCTCTGTCAAAAAGTTGTCGGTTACATTAAATTTAGCAGCAGATTTGCCAGTGTATGCAGTAGGGGATGAAAAGCGCCTTATGCAAATTATTCTAAATGTTGTTGGGAATGCTGTAAAGTTCACAAAAGAAGGGACCATCTTGATGACAGACATTCCAAAGCTTTTCACTAAATTTGCACAAACTCAGTCATTAGCTACTAGAAATTCTGGTGGTGGACTTGGCCTTGCAATTTGTAAGAGATTTGTAAATCTTATGGAGGGGCATATTTGGATTGAAAGTGATGGTCTGGGCAAGGGATGTACTGCCACTTTCATTGTGAAACTCGGATATACAGAACAGTCGAATGAATCTAAGCTGCCTTTTGCGCCTAAAATGCAAGCAAATCATGTGCACAGAGACTTTCCTGGCTTAAAAGTTCTTGTCATGGATGATAACGGGGTCAGCAGGTCAGTGACAGAGGGACTTCTTGTGCACCTGGGATGTGACGTAACATCTGTAAGCTCAAGTGAGGAGTTCTTGCGTGTTATATCTCAGGAGCACAAGGTTGTGTTCATGGATGTATGCATGCCAAGCATAGATGGTTTTGAACTCACTGTCCAGATTCATGAAAAATTTACTAAGCGTCATGAACGGCCAGTATTAGTCGCACTCACTGGTAACATTGACAGGATGACAAAGGAGAGCTGCATGAGGGTTGGTATGGATGGAGTTATACTAAAACCTGTTTCAGTAGATAAGATGAGGAGCGTTTTGTCTGAACTATTAGAGCATCGAGTTCTATTTGAAGCCACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009308 GO:0009408 GO:0009593 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009651 GO:0009690 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009733 GO:0009737 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010087 GO:0010104 GO:0010105 GO:0010119 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016999 GO:0017000 GO:0017144 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0023051 GO:0023052 GO:0023057 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0033993 GO:0034754 GO:0035556 GO:0036211 GO:0038023 GO:0038199 GO:0042221 GO:0042445 GO:0042545 GO:0042562 GO:0042742 GO:0042743 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046483 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048856 GO:0050665 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051239 GO:0051301 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0051740 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0072328 GO:0072593 GO:0097305 GO:0098542 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1903409 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
GAF HATPase_c HisKA Response_reg

Protein Analysis

778

Amino Acids

86.28

Weight (kDa)

6.17

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETR1_N PF25487 97 - 195 8.8e-39 Ethylene receptor 1-like, N-terminal domain
GAF PF01590 238 - 387 2e-11 GAF domain
HisKA PF00512 423 - 488 3.4e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 563 - 622 1.2e-09 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 653 - 764 3.9e-17 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1977
AccI GTMKAC 1 cut(s) 670
AciI CCGC 6 cut(s) 17, 38, 41, 44, 47, 527
AclWI GGATC 1 cut(s) 1209
AcoI YGGCCR 6 cut(s) 272, 489, 936, 1034, 1236, 2174
AcsI RAATTY 6 cut(s) 756, 925, 1570, 1712, 1744, 2153
AfaI GTAC 6 cut(s) 305, 558, 652, 861, 907, 1843
AfiI CCNNNNNNNGG 5 cut(s) 138, 152, 158, 945, 956
AflIII ACRYGT 1 cut(s) 673
AjnI CCWGG 6 cut(s) 151, 970, 1196, 1238, 1945, 2016
AleI CACNNNNGTG 1 cut(s) 111
AloI GAACNNNNNNTCC 2 cut(s) 2121, 2153
AluBI AGCT 9 cut(s) 625, 811, 1042, 1123, 1702, 1736, 1897, 2043, 2226
AluI AGCT 9 cut(s) 625, 811, 1042, 1123, 1702, 1736, 1897, 2043, 2226
Alw21I GWGCWC 5 cut(s) 627, 1521, 1935, 2016, 2082
Alw26I GTCTC 1 cut(s) 1932
Alw44I GTGCAC 3 cut(s) 1517, 1931, 2012
AlwI GGATC 1 cut(s) 1209
AlwNI CAGNNNCTG 1 cut(s) 1378
ApaLI GTGCAC 3 cut(s) 1517, 1931, 2012
ApeKI GCWGC 9 cut(s) 524, 617, 1169, 1345, 1372, 1464, 1577, 1897, 2226
ApoI RAATTY 6 cut(s) 756, 925, 1570, 1712, 1744, 2153
AseI ATTAAT 2 cut(s) 1026, 1428
AspLEI GCGC 3 cut(s) 1377, 1617, 1909
AspS9I GGNCC 2 cut(s) 164, 1674
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 37, 912
AvaII GGWCC 1 cut(s) 1674
BaeGI GKGCMC 4 cut(s) 108, 1521, 1935, 2016
BalI TGGCCA 4 cut(s) 491, 938, 1036, 1238
BanII GRGCYC 1 cut(s) 627
BarI GAAGNNNNNNTAC 2 cut(s) 287, 319
Bbv12I GWGCWC 5 cut(s) 627, 1521, 1935, 2016, 2082
BbvI GCAGC 9 cut(s) 511, 604, 1156, 1332, 1384, 1476, 1589, 1884, 2213
BceAI ACGGC 2 cut(s) 365, 2189
BciT130I CCWGG 6 cut(s) 153, 972, 1198, 1240, 1947, 2018
BciVI GTATCC 1 cut(s) 845
BclI TGATCA 3 cut(s) 27, 879, 1141
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 1932
BfaI CTAG 5 cut(s) 77, 203, 591, 1095, 1740
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 2 cut(s) 1378, 1618
BfuAI ACCTGC 1 cut(s) 1977
BfuI GTATCC 1 cut(s) 845
BglI GCCNNNNNGGC 1 cut(s) 271
BglII AGATCT 1 cut(s) 1441
Bme1390I CCNGG 7 cut(s) 153, 172, 972, 1198, 1240, 1947, 2018
Bme18I GGWCC 1 cut(s) 1674
BmgT120I GGNCC 2 cut(s) 164, 1674
BmiI GGNNCC 1 cut(s) 1675
BmrFI CCNGG 7 cut(s) 153, 172, 972, 1198, 1240, 1947, 2018
BmrI ACTGGG 1 cut(s) 926
BmsI GCATC 4 cut(s) 672, 777, 1065, 2320
BmuI ACTGGG 1 cut(s) 926
BpmI CTGGAG 1 cut(s) 371
Bpu10I CCTNAGC 1 cut(s) 1368
BpuEI CTTGAG 1 cut(s) 2029
BpuMI CCSGG 1 cut(s) 172
BsaAI YACGTR 1 cut(s) 2334
BsaJI CCNNGG 5 cut(s) 152, 510, 971, 1197, 2017
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 152, 158, 945, 956
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 7 cut(s) 805, 845, 932, 1037, 1589, 2177, 2200
Bse3DI GCAATG 3 cut(s) 1106, 1279, 1326
BseBI CCWGG 6 cut(s) 153, 972, 1198, 1240, 1947, 2018
BseDI CCNNGG 5 cut(s) 152, 510, 971, 1197, 2017
BseLI CCNNNNNNNGG 5 cut(s) 138, 152, 158, 945, 956
BseMI GCAATG 3 cut(s) 1106, 1279, 1326
BseMII CTCAG 4 cut(s) 569, 1359, 1739, 2087
BseNI ACTGG 7 cut(s) 805, 845, 932, 1037, 1589, 2177, 2200
BseRI GAGGAG 2 cut(s) 2066, 2299
BseSI GKGCMC 4 cut(s) 108, 1521, 1935, 2016
BseXI GCAGC 9 cut(s) 511, 604, 1156, 1332, 1384, 1476, 1589, 1884, 2213
BsiHKAI GWGCWC 5 cut(s) 627, 1521, 1935, 2016, 2082
BsiSI CCGG 2 cut(s) 171, 275
BslFI GGGAC 4 cut(s) 980, 1259, 1687, 2016
BslI CCNNNNNNNGG 5 cut(s) 138, 152, 158, 945, 956
BsmAI GTCTC 1 cut(s) 1932
BsmFI GGGAC 4 cut(s) 980, 1259, 1687, 2016
BsmI GAATGC 3 cut(s) 5, 475, 1654
Bsp1286I GDGCHC 6 cut(s) 108, 627, 1521, 1935, 2016, 2082
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 8 cut(s) 27, 115, 696, 712, 879, 1141, 1201, 1441
BspACI CCGC 6 cut(s) 17, 38, 41, 44, 47, 527
BspCNI CTCAG 4 cut(s) 570, 1360, 1738, 2086
BspHI TCATGA 3 cut(s) 237, 2146, 2167
BspLI GGNNCC 1 cut(s) 1675
BspMAI CTGCAG 1 cut(s) 622
BspMI ACCTGC 1 cut(s) 1977
BspPI GGATC 1 cut(s) 1209
BsrDI GCAATG 3 cut(s) 1106, 1279, 1326
BsrFI RCCGGY 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 7 cut(s) 805, 845, 932, 1037, 1589, 2177, 2200
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 5 cut(s) 152, 510, 971, 1197, 2017
BssMI GATC 8 cut(s) 27, 115, 696, 712, 879, 1141, 1201, 1441
BssT1I CCWWGG 1 cut(s) 510
Bst2UI CCWGG 6 cut(s) 153, 972, 1198, 1240, 1947, 2018
Bst4CI ACNGT 7 cut(s) 98, 487, 859, 910, 1523, 1881, 2137
Bst6I CTCTTC 3 cut(s) 765, 834, 1176
BstAPI GCANNNNNTGC 2 cut(s) 1265, 1328
BstAUI TGTACA 1 cut(s) 556
BstBAI YACGTR 1 cut(s) 2334
BstC8I GCNNGC 7 cut(s) 168, 276, 473, 1270, 1320, 1920, 2108
BstDEI CTNAG 7 cut(s) 578, 944, 1368, 1725, 1893, 2073, 2160
BstH2I RGCGCY 2 cut(s) 1378, 1618
BstHHI GCGC 3 cut(s) 1377, 1617, 1909
BstKTI GATC 8 cut(s) 30, 118, 699, 715, 882, 1144, 1204, 1444
BstMAI GTCTC 1 cut(s) 1932
BstMBI GATC 8 cut(s) 27, 115, 696, 712, 879, 1141, 1201, 1441
BstNI CCWGG 6 cut(s) 153, 972, 1198, 1240, 1947, 2018
BstNSI RCATGY 6 cut(s) 475, 677, 1109, 1169, 1322, 2110
BstSCI CCNGG 7 cut(s) 151, 170, 970, 1196, 1238, 1945, 2016
BstSFI CTRYAG 1 cut(s) 618
BstSLI GKGCMC 4 cut(s) 108, 1521, 1935, 2016
BstV1I GCAGC 9 cut(s) 511, 604, 1156, 1332, 1384, 1476, 1589, 1884, 2213
BstX2I RGATCY 2 cut(s) 1201, 1441
BstXI CCANNNNNNTGG 3 cut(s) 349, 396, 499
BstYI RGATCY 2 cut(s) 1201, 1441
BsuI GTATCC 1 cut(s) 845
BtsIMutI CAGTG 7 cut(s) 798, 855, 939, 1596, 1998, 2133, 2193
BveI ACCTGC 1 cut(s) 1977
Cac8I GCNNGC 7 cut(s) 168, 276, 473, 1270, 1320, 1920, 2108
CaiI CAGNNNCTG 1 cut(s) 1378
CciI TCATGA 3 cut(s) 237, 2146, 2167
CfoI GCGC 3 cut(s) 1377, 1617, 1909
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 164, 1674
CseI GACGC 3 cut(s) 697, 1256, 2153
Csp6I GTAC 6 cut(s) 304, 557, 651, 860, 906, 1842
CspCI CAANNNNNGTGG 6 cut(s) 468, 503, 724, 759, 1837, 1872
CviQI GTAC 6 cut(s) 304, 557, 651, 860, 906, 1842
DdeI CTNAG 7 cut(s) 578, 944, 1368, 1725, 1893, 2073, 2160
DpnI GATC 8 cut(s) 29, 117, 698, 714, 881, 1143, 1203, 1443
DpnII GATC 8 cut(s) 27, 115, 696, 712, 879, 1141, 1201, 1441
EaeI YGGCCR 6 cut(s) 272, 489, 936, 1034, 1236, 2174
Eam1104I CTCTTC 3 cut(s) 765, 834, 1176
EarI CTCTTC 3 cut(s) 765, 834, 1176
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 625
Eco130I CCWWGG 1 cut(s) 510
Eco147I AGGCCT 1 cut(s) 949
Eco24I GRGCYC 1 cut(s) 627
Eco47I GGWCC 1 cut(s) 1674
Eco53kI GAGCTC 1 cut(s) 625
EcoICRI GAGCTC 1 cut(s) 625
EcoRII CCWGG 6 cut(s) 151, 970, 1196, 1238, 1945, 2016
EcoT14I CCWWGG 1 cut(s) 510
EcoT22I ATGCAT 3 cut(s) 477, 1320, 2108
EcoT38I GRGCYC 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 510
FaqI GGGAC 4 cut(s) 980, 1259, 1687, 2016
FauI CCCGC 1 cut(s) 10
FbaI TGATCA 3 cut(s) 27, 879, 1141
FblI GTMKAC 1 cut(s) 670
FriOI GRGCYC 1 cut(s) 627
FspBI CTAG 5 cut(s) 77, 203, 591, 1095, 1740
GlaI GCGC 3 cut(s) 1376, 1616, 1908
GsuI CTGGAG 1 cut(s) 371
HaeII RGCGCY 2 cut(s) 1378, 1618
HapII CCGG 2 cut(s) 171, 275
HgaI GACGC 3 cut(s) 697, 1256, 2153
HhaI GCGC 3 cut(s) 1377, 1617, 1909
Hin6I GCGC 3 cut(s) 1375, 1615, 1907
HinP1I GCGC 3 cut(s) 1375, 1615, 1907
HindIII AAGCTT 1 cut(s) 1700
HinfI GANTC 7 cut(s) 128, 175, 646, 841, 1184, 1889, 2143
HpaII CCGG 2 cut(s) 171, 275
HphI GGTGA 2 cut(s) 37, 912
Hpy166II GTNNAC 7 cut(s) 653, 671, 1519, 1662, 1757, 1933, 2014
Hpy188I TCNGA 7 cut(s) 313, 645, 701, 1087, 1511, 1868, 2299
Hpy8I GTNNAC 7 cut(s) 653, 671, 1519, 1662, 1757, 1933, 2014
HpyAV CCTTC 3 cut(s) 1186, 1478, 1664
HpyCH4III ACNGT 7 cut(s) 98, 487, 859, 910, 1523, 1881, 2137
HpyCH4IV ACGT 2 cut(s) 2028, 2333
HpyF3I CTNAG 7 cut(s) 578, 944, 1368, 1725, 1893, 2073, 2160
HpySE526I ACGT 2 cut(s) 2028, 2333
HspAI GCGC 3 cut(s) 1375, 1615, 1907
KroI GCCGGC 1 cut(s) 274
KroNI GCCGGC 1 cut(s) 276
Ksp22I TGATCA 3 cut(s) 27, 879, 1141
Kzo9I GATC 8 cut(s) 27, 115, 696, 712, 879, 1141, 1201, 1441
LmnI GCTCC 5 cut(s) 437, 808, 1213, 2077, 2286
Lsp1109I GCAGC 9 cut(s) 511, 604, 1156, 1332, 1384, 1476, 1589, 1884, 2213
LweI GCATC 4 cut(s) 672, 777, 1065, 2320
MaeI CTAG 5 cut(s) 77, 203, 591, 1095, 1740
MaeII ACGT 2 cut(s) 2028, 2333
MaeIII GTNAC 6 cut(s) 1406, 1561, 1993, 2024, 2029, 2198
MalI GATC 8 cut(s) 29, 117, 698, 714, 881, 1143, 1203, 1443
MboI GATC 8 cut(s) 27, 115, 696, 712, 879, 1141, 1201, 1441
MflI RGATCY 2 cut(s) 1201, 1441
MhlI GDGCHC 6 cut(s) 108, 627, 1521, 1935, 2016, 2082
MlsI TGGCCA 4 cut(s) 491, 938, 1036, 1238
MluNI TGGCCA 4 cut(s) 491, 938, 1036, 1238
MlyI GAGTC 2 cut(s) 137, 1193
Mox20I TGGCCA 4 cut(s) 491, 938, 1036, 1238
Mph1103I ATGCAT 3 cut(s) 477, 1320, 2108
MroNI GCCGGC 1 cut(s) 274
MscI TGGCCA 4 cut(s) 491, 938, 1036, 1238
MslI CAYNNNNRTG 6 cut(s) 111, 225, 394, 497, 1110, 1682
Msp20I TGGCCA 4 cut(s) 491, 938, 1036, 1238
MspI CCGG 2 cut(s) 171, 275
MspR9I CCNGG 7 cut(s) 153, 172, 972, 1198, 1240, 1947, 2018
Mva1269I GAATGC 3 cut(s) 5, 475, 1654
MvaI CCWGG 6 cut(s) 153, 972, 1198, 1240, 1947, 2018
NaeI GCCGGC 1 cut(s) 276
NciI CCSGG 1 cut(s) 172
NdeII GATC 8 cut(s) 27, 115, 696, 712, 879, 1141, 1201, 1441
NgoMIV GCCGGC 1 cut(s) 274
NlaIV GGNNCC 1 cut(s) 1675
NmuCI GTSAC 2 cut(s) 1993, 2024
NsiI ATGCAT 3 cut(s) 477, 1320, 2108
NspI RCATGY 6 cut(s) 475, 677, 1109, 1169, 1322, 2110
OliI CACNNNNGTG 1 cut(s) 111
PaeI GCATGC 3 cut(s) 475, 1322, 2110
PagI TCATGA 3 cut(s) 237, 2146, 2167
PceI AGGCCT 1 cut(s) 949
PciI ACATGT 1 cut(s) 673
PctI GAATGC 3 cut(s) 5, 475, 1654
PdiI GCCGGC 1 cut(s) 276
PfeI GAWTC 5 cut(s) 175, 646, 841, 1889, 2143
PleI GAGTC 2 cut(s) 136, 1192
PpsI GAGTC 2 cut(s) 136, 1192
Ppu21I YACGTR 1 cut(s) 2334
PscI ACATGT 1 cut(s) 673
PshBI ATTAAT 2 cut(s) 1026, 1428
Psp124BI GAGCTC 1 cut(s) 627
Psp6I CCWGG 6 cut(s) 151, 970, 1196, 1238, 1945, 2016
PspGI CCWGG 6 cut(s) 151, 970, 1196, 1238, 1945, 2016
PspN4I GGNNCC 1 cut(s) 1675
PspPI GGNCC 2 cut(s) 164, 1674
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 1378
PsuI RGATCY 2 cut(s) 1201, 1441
RsaI GTAC 6 cut(s) 305, 558, 652, 861, 907, 1843
RsaNI GTAC 6 cut(s) 304, 557, 651, 860, 906, 1842
RseI CAYNNNNRTG 6 cut(s) 111, 225, 394, 497, 1110, 1682
SacI GAGCTC 1 cut(s) 627
Sau3AI GATC 8 cut(s) 27, 115, 696, 712, 879, 1141, 1201, 1441
Sau96I GGNCC 2 cut(s) 164, 1674
SchI GAGTC 2 cut(s) 137, 1193
ScrFI CCNGG 7 cut(s) 153, 172, 972, 1198, 1240, 1947, 2018
SduI GDGCHC 6 cut(s) 108, 627, 1521, 1935, 2016, 2082
SfaNI GCATC 4 cut(s) 672, 777, 1065, 2320
SfcI CTRYAG 1 cut(s) 618
SinI GGWCC 1 cut(s) 1674
SmiMI CAYNNNNRTG 6 cut(s) 111, 225, 394, 497, 1110, 1682
SmlI CTYRAG 1 cut(s) 2044
SmoI CTYRAG 1 cut(s) 2044
SphI GCATGC 3 cut(s) 475, 1322, 2110
SseBI AGGCCT 1 cut(s) 949
SsiI CCGC 6 cut(s) 17, 38, 41, 44, 47, 527
SspI AATATT 2 cut(s) 1176, 1222
SspMI CTAG 5 cut(s) 77, 203, 591, 1095, 1740
SstI GAGCTC 1 cut(s) 627
StuI AGGCCT 1 cut(s) 949
StyD4I CCNGG 7 cut(s) 151, 170, 970, 1196, 1238, 1945, 2016
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 7 cut(s) 98, 487, 859, 910, 1523, 1881, 2137
TaiI ACGT 2 cut(s) 2031, 2336
TaqI TCGA 6 cut(s) 9, 627, 715, 1476, 1883, 2314
TatI WGTACW 3 cut(s) 303, 556, 1841
TauI GCSGC 3 cut(s) 41, 44, 47
TfiI GAWTC 5 cut(s) 175, 646, 841, 1889, 2143
TscAI CASTG 7 cut(s) 805, 862, 939, 1596, 1998, 2140, 2200
TseFI GTSAC 2 cut(s) 1993, 2024
TseI GCWGC 9 cut(s) 524, 617, 1169, 1345, 1372, 1464, 1577, 1897, 2226
Tsp45I GTSAC 2 cut(s) 1993, 2024
TspRI CASTG 7 cut(s) 805, 862, 939, 1596, 1998, 2140, 2200
VneI GTGCAC 3 cut(s) 1517, 1931, 2012
VpaK11BI GGWCC 1 cut(s) 1674
VspI ATTAAT 2 cut(s) 1026, 1428
XapI RAATTY 6 cut(s) 756, 925, 1570, 1712, 1744, 2153
XceI RCATGY 6 cut(s) 475, 677, 1109, 1169, 1322, 2110
XmiI GTMKAC 1 cut(s) 670
XspI CTAG 5 cut(s) 77, 203, 591, 1095, 1740
Zsp2I ATGCAT 3 cut(s) 477, 1320, 2108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.