RLG00000001686

May act early in the ethylene signal transduction pathway, possibly as an ethylene receptor, or as a regulator of the pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003094
Physical Location & Seq
Forward (+)
19105585 .. 19111171
5587 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001686

Sequence Viewer

Length: 2223 bp
ATGGAGGGGTGCTGCATTGAGCCACAATGGCCGGCTGATGAATTGTTGATGAAGTATCAGTACATTTCAGATTTCTTTATTGCACTTGCTTATTTCTCCATCCCTCTGGAGTTAATCTACTTTGTCAAGAAATCTGCCGTGTTTCCATACAAATGGGTTCTTGTTCAGTTTGGTGCTTTTATTGTATTGTGTGGAGCGACACATCTTATTAACTTATGGACTTTTAGCGTGCATTCAAGAACTGTGGCCATAGTAATGGCTACTGCCAAGGTTTTCACTGCTGCGGTGTCATGTGCTACTGCCCTCATGCTTGTTCATATTATTCCTGATTTACTGAGTGTTAAAACTAGAGAGTTATTCCTGAAGAACAAGGCTGCAGAGCTCGACAGAGAAATGGGTTTGATTCGTACACAGGAAGAAACAGGTAGACATGTCAGGATGCTGACGCATGAGATCAGAAGCACTCTTGATCGGCATACTATACTGAAAACCACACTTGTTGAATTAGGTAGAATTTTGACATTGGAAGAGTGTGCCTTGTGGATGCCAACACGCACTGGGTTGGAGCTTCAACTATCCTATACTCTTCATCAGCAGAATCCAGTAGGATACACTGTACCCATCCATCTTCCTGTGATCAATCAAGTATTTAATAACAACGGTACGGTAAAAATATCACCGAATTCTCCAGTGGCCAGACTAAGGCCTATTGCTGGAAAACACATTCCTGGGGAGGTTGTTGCAGTTCGTGTCCCACTCCTACATCTCTCTAACTTCCAAATTAATGACTGGCCAGAGCTCTCAACAAAACGCTATGCTTTGATGGTGTTGATGCTTCCCTCTGATAGTGCTAGGCAATGGCATGTCCATGAGTTAGAGCTGGTGGAGGTAGTTGCTGATCAGGTGGCAGTTGCTCTTTCACATGCTGCAATATTAGAAGAGTCAAGGAAGGCCAGGGATCTTCTTGTGAAGCAGAATATTGCACTTGATTTGGCCAGGAGGGATGCTGAAGTTGCAATTTGTGCTCGCAATGATTTCTTGGCTGTTATGAACCATGAGATGAGAACTCCCATGCATGCAATTATTGCCCTTTCTTCCTTGCTGCAAGAAACTGAACTGACACCTGAGCAGCGCCTGATGGTTGAAACAATACTAAAGAGCAGTAACCTTTTGGCTACCCTCATTAATGATGTATTAGATCTTTCAAGGCTTGAAGATGGCAGCCTTCAACTCGATATAGCAACTTTTAACCTCCATTCTTTATTCAGAGAGGTGCACAGTTTAGTTAAGCCTGTTGCCTCTGTCAAAAAGTTGTCGGTTACATTAAATTTAGCAGCAGATTTGCCAGTATATGCGGTAGGTGATGAAAAGCGCCTTATGCAAACTATTCTAAATGTTGTCGGGAATGCTGTGAAGTTCACAAAAGAAGGGACCATCTCGATTGCAGTTTTTGTTGCCAAATCAGAATCCTTAAGAGATTTCCGGGCTCCTGATTTTTATCCAGTGCAGAGTGATAATCACTTTTATCTGCGGGTACAGGTAAAAGATTCAGGATCTGGAATTAATCCCCAGGATATTCCAAAGCTTTTCACCAAATTCGCACAAACTCAGTCATTAGCTACTAGAAATTCTGGTGGTAGTGGACTTGGCCTTGCAATTTGTAAGAGATTTGTAAATCTTATGGAGGGGCACATTTGGATTGAAAGTGATGGTCTGGGCAAGGGATGTACTGCCACTTTCATTGTGAAGCTCGGATATTCAGAACAGTCGAATGAATCTAAGCTGCCTTTCGTGCCTAAAATGCAAGCAAATCATGTACATTCAGACTTTCCTGGGTTAAAAGTTCTTGTCATGGATGATAACGGGGTCAGCAGGTCAGCGACAGAGGGACTCCTTGTGCACCTGGGATGTGATGTAACATCTGTAAGCTCAAGTGAGGAGTTCTTGCGTGTTATATCTCAGGAACACAAGGTTGTGTTCATGGATGTATGCATGCCTAGCATAGATGGTTTTGAATTAACTGTCCAGATTCATGAAAAATTTACTAAACGTCATGAACGGCCAGTGTTAGTCGCACTCACTGGAAACATGGACAGGATGACAAAGGAGAGCTGCATGAGGGTTGGTATGGATGGAGTTATACTAAAACCTGTTTCAGTAGATAAGATGAGGAGTGTTTTGTCAGAACTATTAGAGCATCGAGTTCTATTTGAAGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009308 GO:0009408 GO:0009593 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009651 GO:0009690 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009733 GO:0009737 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010087 GO:0010104 GO:0010105 GO:0010119 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016999 GO:0017000 GO:0017144 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0023051 GO:0023052 GO:0023057 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0033993 GO:0034754 GO:0035556 GO:0036211 GO:0038023 GO:0038199 GO:0042221 GO:0042445 GO:0042545 GO:0042562 GO:0042742 GO:0042743 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046483 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048856 GO:0050665 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051239 GO:0051301 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0051740 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0072328 GO:0072593 GO:0097305 GO:0098542 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1903409 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
GAF HATPase_c HisKA Response_reg

Protein Analysis

741

Amino Acids

82.74

Weight (kDa)

6.61

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETR1_N PF25487 16 - 114 4.6e-38 Ethylene receptor 1-like, N-terminal domain
GAF PF01590 157 - 306 2e-11 GAF domain
HisKA PF00512 342 - 407 3.2e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 454 - 584 6.1e-25 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 615 - 726 3.2e-17 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1863
AccI GTMKAC 1 cut(s) 427
AciI CCGC 3 cut(s) 284, 1355, 1531
AclWI GGATC 2 cut(s) 966, 1561
AcoI YGGCCR 6 cut(s) 29, 246, 693, 791, 993, 2060
AcsI RAATTY 6 cut(s) 513, 682, 1327, 1595, 1627, 2039
AcuI CTGAAG 2 cut(s) 383, 1029
AfaI GTAC 7 cut(s) 62, 409, 618, 664, 1536, 1729, 1818
AfiI CCNNNNNNNGG 2 cut(s) 702, 713
AflII CTTAAG 1 cut(s) 1471
AflIII ACRYGT 1 cut(s) 430
AjnI CCWGG 6 cut(s) 727, 953, 995, 1569, 1831, 1902
Alw21I GWGCWC 5 cut(s) 384, 801, 1027, 1278, 1902
Alw44I GTGCAC 2 cut(s) 1274, 1898
AlwI GGATC 2 cut(s) 966, 1561
AlwNI CAGNNNCTG 2 cut(s) 1135, 1556
AoxI GGCC 9 cut(s) 29, 246, 693, 704, 791, 951, 993, 1648, 2060
ApaLI GTGCAC 2 cut(s) 1274, 1898
ApoI RAATTY 6 cut(s) 513, 682, 1327, 1595, 1627, 2039
AseI ATTAAT 3 cut(s) 783, 1185, 1563
AspLEI GCGC 2 cut(s) 1134, 1374
AspS9I GGNCC 1 cut(s) 1431
AsuC2I CCSGG 1 cut(s) 1484
AsuHPI GGTGA 3 cut(s) 669, 1373, 1582
AvaII GGWCC 1 cut(s) 1431
BaeGI GKGCMC 3 cut(s) 1278, 1692, 1902
BalI TGGCCA 4 cut(s) 248, 695, 793, 995
BanII GRGCYC 3 cut(s) 384, 801, 1489
BarI GAAGNNNNNNTAC 2 cut(s) 44, 76
Bbv12I GWGCWC 5 cut(s) 384, 801, 1027, 1278, 1902
BbvI GCAGC 9 cut(s) 268, 361, 913, 1089, 1141, 1233, 1346, 1770, 2099
BceAI ACGGC 2 cut(s) 122, 2075
BciT130I CCWGG 6 cut(s) 729, 955, 997, 1571, 1833, 1904
BciVI GTATCC 1 cut(s) 602
BclI TGATCA 2 cut(s) 636, 898
BcnI CCSGG 1 cut(s) 1484
BfaI CTAG 4 cut(s) 348, 852, 1623, 1998
BfmI CTRYAG 1 cut(s) 375
BfoI RGCGCY 2 cut(s) 1135, 1375
BfrI CTTAAG 1 cut(s) 1471
BfuAI ACCTGC 1 cut(s) 1863
BfuI GTATCC 1 cut(s) 602
BglI GCCNNNNNGGC 1 cut(s) 28
BglII AGATCT 1 cut(s) 1198
Bme1390I CCNGG 7 cut(s) 729, 955, 997, 1484, 1571, 1833, 1904
Bme18I GGWCC 1 cut(s) 1431
BmgT120I GGNCC 1 cut(s) 1431
BmiI GGNNCC 2 cut(s) 1432, 1488
BmrFI CCNGG 7 cut(s) 729, 955, 997, 1484, 1571, 1833, 1904
BmrI ACTGGG 1 cut(s) 567
BmsI GCATC 5 cut(s) 429, 534, 822, 994, 2206
BmuI ACTGGG 1 cut(s) 567
BpmI CTGGAG 2 cut(s) 128, 672
Bpu10I CCTNAGC 1 cut(s) 1125
BpuEI CTTGAG 1 cut(s) 1915
BpuMI CCSGG 1 cut(s) 1484
BsaBI GATNNNNATC 1 cut(s) 1497
BsaJI CCNNGG 6 cut(s) 267, 728, 954, 1569, 1832, 1903
Bsc4I CCNNNNNNNGG 2 cut(s) 702, 713
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 8 cut(s) 562, 602, 689, 794, 1346, 1502, 2063, 2086
Bse3DI GCAATG 2 cut(s) 863, 1036
Bse8I GATNNNNATC 1 cut(s) 1497
BseBI CCWGG 6 cut(s) 729, 955, 997, 1571, 1833, 1904
BseDI CCNNGG 6 cut(s) 267, 728, 954, 1569, 1832, 1903
BseJI GATNNNNATC 1 cut(s) 1497
BseLI CCNNNNNNNGG 2 cut(s) 702, 713
BseMI GCAATG 2 cut(s) 863, 1036
BseMII CTCAG 4 cut(s) 326, 1116, 1622, 1973
BseNI ACTGG 8 cut(s) 562, 602, 689, 794, 1346, 1502, 2063, 2086
BseRI GAGGAG 2 cut(s) 1952, 2185
BseSI GKGCMC 3 cut(s) 1278, 1692, 1902
BseXI GCAGC 9 cut(s) 268, 361, 913, 1089, 1141, 1233, 1346, 1770, 2099
BsgI GTGCAG 1 cut(s) 1526
BshFI GGCC 9 cut(s) 31, 248, 695, 706, 793, 953, 995, 1650, 2062
BsiHKAI GWGCWC 5 cut(s) 384, 801, 1027, 1278, 1902
BsiSI CCGG 2 cut(s) 32, 1483
BslFI GGGAC 3 cut(s) 737, 1444, 1902
BslI CCNNNNNNNGG 2 cut(s) 702, 713
BsmFI GGGAC 3 cut(s) 737, 1444, 1902
BsmI GAATGC 2 cut(s) 232, 1411
BsnI GGCC 9 cut(s) 31, 248, 695, 706, 793, 953, 995, 1650, 2062
Bsp1286I GDGCHC 7 cut(s) 384, 801, 1027, 1278, 1489, 1692, 1902
Bsp1407I TGTACA 1 cut(s) 1816
Bsp143I GATC 7 cut(s) 453, 469, 636, 898, 958, 1198, 1553
BspACI CCGC 3 cut(s) 284, 1355, 1531
BspANI GGCC 9 cut(s) 31, 248, 695, 706, 793, 953, 995, 1650, 2062
BspCNI CTCAG 4 cut(s) 327, 1117, 1621, 1972
BspHI TCATGA 2 cut(s) 2032, 2053
BspLI GGNNCC 2 cut(s) 1432, 1488
BspMAI CTGCAG 1 cut(s) 379
BspMI ACCTGC 1 cut(s) 1863
BspPI GGATC 2 cut(s) 966, 1561
BspTI CTTAAG 1 cut(s) 1471
BsrDI GCAATG 2 cut(s) 863, 1036
BsrFI RCCGGY 1 cut(s) 31
BsrGI TGTACA 1 cut(s) 1816
BsrI ACTGG 8 cut(s) 562, 602, 689, 794, 1346, 1502, 2063, 2086
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 6 cut(s) 267, 728, 954, 1569, 1832, 1903
BssMI GATC 7 cut(s) 453, 469, 636, 898, 958, 1198, 1553
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 6 cut(s) 729, 955, 997, 1571, 1833, 1904
Bst4CI ACNGT 7 cut(s) 244, 616, 662, 667, 1280, 1767, 2023
Bst6I CTCTTC 3 cut(s) 522, 591, 933
BstAFI CTTAAG 1 cut(s) 1471
BstAPI GCANNNNNTGC 2 cut(s) 1022, 1085
BstAUI TGTACA 1 cut(s) 1816
BstC8I GCNNGC 6 cut(s) 33, 230, 1027, 1077, 1806, 1994
BstDEI CTNAG 6 cut(s) 335, 701, 1125, 1608, 1779, 1959
BstH2I RGCGCY 2 cut(s) 1135, 1375
BstHHI GCGC 2 cut(s) 1134, 1374
BstKTI GATC 7 cut(s) 456, 472, 639, 901, 961, 1201, 1556
BstMBI GATC 7 cut(s) 453, 469, 636, 898, 958, 1198, 1553
BstMWI GCNNNNNNNGC 8 cut(s) 28, 1013, 1022, 1085, 1378, 1792, 1801, 1998
BstNI CCWGG 6 cut(s) 729, 955, 997, 1571, 1833, 1904
BstNSI RCATGY 5 cut(s) 434, 866, 926, 1079, 1996
BstSCI CCNGG 7 cut(s) 727, 953, 995, 1482, 1569, 1831, 1902
BstSFI CTRYAG 1 cut(s) 375
BstSLI GKGCMC 3 cut(s) 1278, 1692, 1902
BstV1I GCAGC 9 cut(s) 268, 361, 913, 1089, 1141, 1233, 1346, 1770, 2099
BstX2I RGATCY 3 cut(s) 958, 1198, 1553
BstXI CCANNNNNNTGG 3 cut(s) 106, 153, 256
BstYI RGATCY 3 cut(s) 958, 1198, 1553
BsuI GTATCC 1 cut(s) 602
BsuRI GGCC 9 cut(s) 31, 248, 695, 706, 793, 953, 995, 1650, 2062
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 7 cut(s) 276, 555, 612, 696, 1509, 2070, 2079
BveI ACCTGC 1 cut(s) 1863
Cac8I GCNNGC 6 cut(s) 33, 230, 1027, 1077, 1806, 1994
CaiI CAGNNNCTG 2 cut(s) 1135, 1556
CciI TCATGA 2 cut(s) 2032, 2053
CfoI GCGC 2 cut(s) 1134, 1374
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 1431
CseI GACGC 1 cut(s) 454
Csp6I GTAC 7 cut(s) 61, 408, 617, 663, 1535, 1728, 1817
CspCI CAANNNNNGTGG 6 cut(s) 225, 260, 481, 516, 1723, 1758
CviQI GTAC 7 cut(s) 61, 408, 617, 663, 1535, 1728, 1817
DdeI CTNAG 6 cut(s) 335, 701, 1125, 1608, 1779, 1959
DpnI GATC 7 cut(s) 455, 471, 638, 900, 960, 1200, 1555
DpnII GATC 7 cut(s) 453, 469, 636, 898, 958, 1198, 1553
EaeI YGGCCR 6 cut(s) 29, 246, 693, 791, 993, 2060
Eam1104I CTCTTC 3 cut(s) 522, 591, 933
EarI CTCTTC 3 cut(s) 522, 591, 933
Ecl136II GAGCTC 2 cut(s) 382, 799
Eco130I CCWWGG 1 cut(s) 267
Eco147I AGGCCT 1 cut(s) 706
Eco24I GRGCYC 3 cut(s) 384, 801, 1489
Eco47I GGWCC 1 cut(s) 1431
Eco53kI GAGCTC 2 cut(s) 382, 799
Eco57I CTGAAG 2 cut(s) 383, 1029
EcoICRI GAGCTC 2 cut(s) 382, 799
EcoRI GAATTC 1 cut(s) 682
EcoRII CCWGG 6 cut(s) 727, 953, 995, 1569, 1831, 1902
EcoT14I CCWWGG 1 cut(s) 267
EcoT22I ATGCAT 2 cut(s) 1077, 1994
EcoT38I GRGCYC 3 cut(s) 384, 801, 1489
ErhI CCWWGG 1 cut(s) 267
FaqI GGGAC 3 cut(s) 737, 1444, 1902
FauI CCCGC 1 cut(s) 1524
FbaI TGATCA 2 cut(s) 636, 898
FblI GTMKAC 1 cut(s) 427
FriOI GRGCYC 3 cut(s) 384, 801, 1489
FspBI CTAG 4 cut(s) 348, 852, 1623, 1998
GlaI GCGC 2 cut(s) 1133, 1373
GsuI CTGGAG 2 cut(s) 128, 672
HaeII RGCGCY 2 cut(s) 1135, 1375
HaeIII GGCC 9 cut(s) 31, 248, 695, 706, 793, 953, 995, 1650, 2062
HapII CCGG 2 cut(s) 32, 1483
HgaI GACGC 1 cut(s) 454
HhaI GCGC 2 cut(s) 1134, 1374
Hin6I GCGC 2 cut(s) 1132, 1372
HinP1I GCGC 2 cut(s) 1132, 1372
HindIII AAGCTT 1 cut(s) 1583
HinfI GANTC 8 cut(s) 403, 598, 941, 1466, 1547, 1775, 1890, 2029
HpaII CCGG 2 cut(s) 32, 1483
HphI GGTGA 3 cut(s) 669, 1373, 1582
Hpy166II GTNNAC 6 cut(s) 410, 428, 1276, 1419, 1643, 1900
Hpy188I TCNGA 9 cut(s) 70, 458, 844, 1268, 1465, 1754, 1762, 1825, 2185
Hpy8I GTNNAC 6 cut(s) 410, 428, 1276, 1419, 1643, 1900
HpyAV CCTTC 3 cut(s) 943, 1235, 1421
HpyCH4III ACNGT 7 cut(s) 244, 616, 662, 667, 1280, 1767, 2023
HpyCH4IV ACGT 1 cut(s) 2050
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 1013, 1022, 1085, 1378, 1792, 1801, 1998
HpyF3I CTNAG 6 cut(s) 335, 701, 1125, 1608, 1779, 1959
HpySE526I ACGT 1 cut(s) 2050
HspAI GCGC 2 cut(s) 1132, 1372
KroI GCCGGC 1 cut(s) 31
KroNI GCCGGC 1 cut(s) 33
Ksp22I TGATCA 2 cut(s) 636, 898
Kzo9I GATC 7 cut(s) 453, 469, 636, 898, 958, 1198, 1553
LmnI GCTCC 3 cut(s) 194, 565, 1492
Lsp1109I GCAGC 9 cut(s) 268, 361, 913, 1089, 1141, 1233, 1346, 1770, 2099
LweI GCATC 5 cut(s) 429, 534, 822, 994, 2206
MaeI CTAG 4 cut(s) 348, 852, 1623, 1998
MaeII ACGT 1 cut(s) 2050
MaeIII GTNAC 3 cut(s) 1163, 1318, 1915
MalI GATC 7 cut(s) 455, 471, 638, 900, 960, 1200, 1555
MboI GATC 7 cut(s) 453, 469, 636, 898, 958, 1198, 1553
MboII GAAGA 9 cut(s) 376, 428, 539, 578, 620, 950, 953, 1086, 1226
MflI RGATCY 3 cut(s) 958, 1198, 1553
MhlI GDGCHC 7 cut(s) 384, 801, 1027, 1278, 1489, 1692, 1902
MlsI TGGCCA 4 cut(s) 248, 695, 793, 995
MluNI TGGCCA 4 cut(s) 248, 695, 793, 995
MlyI GAGTC 2 cut(s) 950, 1884
MmeI TCCRAC 1 cut(s) 543
Mox20I TGGCCA 4 cut(s) 248, 695, 793, 995
Mph1103I ATGCAT 2 cut(s) 1077, 1994
MroNI GCCGGC 1 cut(s) 31
MscI TGGCCA 4 cut(s) 248, 695, 793, 995
MslI CAYNNNNRTG 3 cut(s) 151, 254, 867
Msp20I TGGCCA 4 cut(s) 248, 695, 793, 995
MspCI CTTAAG 1 cut(s) 1471
MspI CCGG 2 cut(s) 32, 1483
MspR9I CCNGG 7 cut(s) 729, 955, 997, 1484, 1571, 1833, 1904
Mva1269I GAATGC 2 cut(s) 232, 1411
MvaI CCWGG 6 cut(s) 729, 955, 997, 1571, 1833, 1904
MwoI GCNNNNNNNGC 8 cut(s) 28, 1013, 1022, 1085, 1378, 1792, 1801, 1998
NaeI GCCGGC 1 cut(s) 33
NciI CCSGG 1 cut(s) 1484
NdeII GATC 7 cut(s) 453, 469, 636, 898, 958, 1198, 1553
NgoMIV GCCGGC 1 cut(s) 31
NlaIV GGNNCC 2 cut(s) 1432, 1488
NsiI ATGCAT 2 cut(s) 1077, 1994
NspI RCATGY 5 cut(s) 434, 866, 926, 1079, 1996
PaeI GCATGC 2 cut(s) 1079, 1996
PagI TCATGA 2 cut(s) 2032, 2053
PceI AGGCCT 1 cut(s) 706
PciI ACATGT 1 cut(s) 430
PctI GAATGC 2 cut(s) 232, 1411
PdiI GCCGGC 1 cut(s) 33
PfeI GAWTC 6 cut(s) 403, 598, 1466, 1547, 1775, 2029
PleI GAGTC 2 cut(s) 949, 1884
PpsI GAGTC 2 cut(s) 949, 1884
PscI ACATGT 1 cut(s) 430
PshBI ATTAAT 3 cut(s) 783, 1185, 1563
Psp124BI GAGCTC 2 cut(s) 384, 801
Psp6I CCWGG 6 cut(s) 727, 953, 995, 1569, 1831, 1902
PspGI CCWGG 6 cut(s) 727, 953, 995, 1569, 1831, 1902
PspN4I GGNNCC 2 cut(s) 1432, 1488
PspPI GGNCC 1 cut(s) 1431
PstI CTGCAG 1 cut(s) 379
PstNI CAGNNNCTG 2 cut(s) 1135, 1556
PsuI RGATCY 3 cut(s) 958, 1198, 1553
RsaI GTAC 7 cut(s) 62, 409, 618, 664, 1536, 1729, 1818
RsaNI GTAC 7 cut(s) 61, 408, 617, 663, 1535, 1728, 1817
RseI CAYNNNNRTG 3 cut(s) 151, 254, 867
SacI GAGCTC 2 cut(s) 384, 801
Sau3AI GATC 7 cut(s) 453, 469, 636, 898, 958, 1198, 1553
Sau96I GGNCC 1 cut(s) 1431
SchI GAGTC 2 cut(s) 950, 1884
ScrFI CCNGG 7 cut(s) 729, 955, 997, 1484, 1571, 1833, 1904
SduI GDGCHC 7 cut(s) 384, 801, 1027, 1278, 1489, 1692, 1902
SfaNI GCATC 5 cut(s) 429, 534, 822, 994, 2206
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 1 cut(s) 1431
SmiMI CAYNNNNRTG 3 cut(s) 151, 254, 867
SmlI CTYRAG 2 cut(s) 1471, 1930
SmoI CTYRAG 2 cut(s) 1471, 1930
SphI GCATGC 2 cut(s) 1079, 1996
SseBI AGGCCT 1 cut(s) 706
SsiI CCGC 3 cut(s) 284, 1355, 1531
SspI AATATT 2 cut(s) 933, 979
SspMI CTAG 4 cut(s) 348, 852, 1623, 1998
SstI GAGCTC 2 cut(s) 384, 801
StuI AGGCCT 1 cut(s) 706
StyD4I CCNGG 7 cut(s) 727, 953, 995, 1482, 1569, 1831, 1902
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 7 cut(s) 244, 616, 662, 667, 1280, 1767, 2023
TaiI ACGT 1 cut(s) 2053
TaqI TCGA 5 cut(s) 384, 1233, 1439, 1769, 2200
TatI WGTACW 3 cut(s) 60, 1727, 1816
TfiI GAWTC 6 cut(s) 403, 598, 1466, 1547, 1775, 2029
TscAI CASTG 7 cut(s) 283, 562, 619, 696, 1509, 2070, 2086
TspRI CASTG 7 cut(s) 283, 562, 619, 696, 1509, 2070, 2086
Vha464I CTTAAG 1 cut(s) 1471
VneI GTGCAC 2 cut(s) 1274, 1898
VpaK11BI GGWCC 1 cut(s) 1431
VspI ATTAAT 3 cut(s) 783, 1185, 1563
XapI RAATTY 6 cut(s) 513, 682, 1327, 1595, 1627, 2039
XceI RCATGY 5 cut(s) 434, 866, 926, 1079, 1996
XmiI GTMKAC 1 cut(s) 427
XspI CTAG 4 cut(s) 348, 852, 1623, 1998
Zsp2I ATGCAT 2 cut(s) 1077, 1994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.