MD02G1161700.v1.1

May act early in the ethylene signal transduction pathway, possibly as an ethylene receptor, or as a regulator of the pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003094
Physical Location & Seq
Forward (+)
13579736 .. 13585898
6163 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1161700.v1.1.491

Sequence Viewer

Length: 2232 bp
ATGATCATGGAGGCATGCAACTGTATTGAGCCACAATGGCCGGCAGATGAATTGCTGATGAAATATCAGTACATTTCGGATTTCTTTATTGCACTTGCTTATTTCTCCATCCCTCTGGAGTTGATCTACTTTGTCAAGAAATCTGCTGTGTTTCCATACAGATGGGTCCTCGTACAGTTTGGTGCTTTCATTGTACTGTGTGGGGCGACTCATCTTATCAACTTATGGACGTTCAACATGCATTCAAGAACTGTGGCCATAGTAATGACCACGGCTAAGGTTTTAACAGCGGTGGTGTCATGTGCAACTGCCCTTATGCTTGTACATATTATACCTGATTTATTGAGTGTTAAAACTAGAGAACTATTCTTGAAGAACAAGGCTGCAGAGCTTGATAGAGAAATGGGCCTTATTCGAACACAGGAGGAAACAGGTCGACATGTCAGGATGTTGACCCATGAAATTAGAAGCACTCTTGATAGACATACTATACTTAAAACTACACTTGTCGAGTTAGGTAGAACTCTGGCATTGGAAGAGTGTGCCTTGTGGATGCCAACGCGTACTGGGTTAGAGCTTCAACTATCCTATACACTCCGTCAACAGAATCCAGTAGGATATACTGTGCCCATTCATCTTCCTGTGGTCAATCAAGTATTTAGTAGCAACTGTGCAGTAAAAATATCACCTAATTCTCCAGTGGCACGACTGAGGCAACTTGCAGGAAAGCACATTCCTGGGGAAGTTGTTGCAGTTCGTGTGCCCCTTTTACATCTCTCTAACTTCCAAATTAATGACTGGCCAGAGCTCTCAACAAAACGCTATGCTTTGATGGTCTTGATGCTTCCCTCAGACAGTGCAAGGCAATGGCACGTTCACGAGTTAGAATTGGTTGAAGTAGTTGCTGATCAGGTTGCAGTTGCTCTGTCACATGCTGCAATATTAGAAGAGTCAATGAGGGCAAGGGATCTCCTTATGGAGCAGAATATTGCGCTTGATCTGGCCAGAAGAGAAGCGGAAACTGCAATTCGTGCTCGCAATGATTTCTTGGCTGTTATGAACCATGAAATGAGAACTCCCATGCATGCAATTATTGCTCTTTCTTCTTTGCTGCAGGAAACTGAGCTGACACCTGAACAGCGCCTCATGGTTGAAACGATATTAAAGAGCAGCAACCTATTGGCTACCCTTATAAATGATGTTTTGGATCTTTCAAGGCTCGAAGATGGCAGCCTTCAACTTGACATAGCAACTTTTAACCTCCATTCCATATTCAGAGAGGTTCATAATTTAATTAAGCCTGTTGCATCTGTCAAAAAGTTGTCGGTTGCTTTGAATATAGCTACAGATTTGCCAGTGTATGCCATAGGTGATGAAAAGCGTCTTATGCAAATTATTCTAAATGTTGTTGGGAATGCTGTGAAGTTCTCAAAAGAAGGGAGCATCTCAATCACAGCTTATGTTGCAAAATCAGAATCTTTACGAGATTTCCGGGCTCCTGATTTCTTTCCAGTACAGAGTGATAATCACTTTTATCTGCGTGTACAGGTCAAAGATTCAGGATTGGGAATCAATCCTCAGGATATCCCAAATCTATTTACCAAATTCGCTCAAACTCAGACTTCTGCTTCTCAAAATTCTGGTGGTAGTGGGCTTGGCCTTGCAATTTGTAAGAGATTTGTAAATCTTATGGAAGGGCATATTTGGATTGAAAGTGAAGGTCTTGGCAAGGGATGCACAGCCACTTTCATTGTGAAACTCGGATTTCCAGAGCGTTCAAATGAGTTTAAGCAGCCTTTTGCACCCAAGCTGCAAGCAAATCATGTTCAAACAAACTTTCCAGGGTTGAAAGTCCTTGTTATGGATGATAATGGGGTCAGCAGGTCAGTGACAAAGGGACTTCTTGTGCACCTAGGATGTGATGTAACAACTGTGAGCTCAATTCATGAGTTCTTGCATGTTATTTCGCAAGAGCACAAGGTGGTGTTCGTGGATGTGTGCATGCCAGGTATAGATGGCTATGAAGTTGCTGTTCGTATACATGAAAAGTTCACAAAACGTCATGAACGGCCTGTATTAGTAGCACTTACCGGAAGCATAGACAAGATGACAAAGGAGAACTGCATGAGGGTTGGTATGGACGGAGTCATACTAAAACCGGTTTCAGTTGATAAAATGAGGAGCGTTTTATCAGAACTGTTAGAGCATCGAGTCCTATTCGAGGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009308 GO:0009408 GO:0009593 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009651 GO:0009690 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009733 GO:0009737 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010087 GO:0010104 GO:0010105 GO:0010119 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016999 GO:0017000 GO:0017144 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0023051 GO:0023052 GO:0023057 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0033993 GO:0034754 GO:0035556 GO:0036211 GO:0038023 GO:0038199 GO:0042221 GO:0042445 GO:0042545 GO:0042562 GO:0042742 GO:0042743 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046483 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048856 GO:0050665 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051239 GO:0051301 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0051740 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0072328 GO:0072593 GO:0097305 GO:0098542 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1903409 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
GAF HATPase_c HisKA Response_reg

Protein Analysis

744

Amino Acids

83.3

Weight (kDa)

6.82

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETR1_N PF25487 19 - 117 8.4e-40 Ethylene receptor 1-like, N-terminal domain
GAF PF01590 160 - 309 9.5e-12 GAF domain
HisKA PF00512 345 - 410 3.2e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 457 - 587 1.6e-25 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 618 - 729 8.4e-17 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1193
Acc36I ACCTGC 1 cut(s) 1872
AccI GTMKAC 2 cut(s) 436, 2038
AccII CGCG 1 cut(s) 562
AciI CCGC 2 cut(s) 290, 1016
AclWI GGATC 2 cut(s) 975, 1215
AcoI YGGCCR 4 cut(s) 38, 255, 800, 1002
AcsI RAATTY 2 cut(s) 1604, 1636
AdeI CACNNNGTG 1 cut(s) 1981
AfaI GTAC 7 cut(s) 71, 174, 195, 324, 565, 1515, 1545
AfiI CCNNNNNNNGG 2 cut(s) 1861, 2221
AflIII ACRYGT 2 cut(s) 439, 560
AgeI ACCGGT 1 cut(s) 2158
AjnI CCWGG 3 cut(s) 736, 1840, 2005
AluBI AGCT 8 cut(s) 391, 577, 808, 1126, 1343, 1457, 1810, 1938
AluI AGCT 8 cut(s) 391, 577, 808, 1126, 1343, 1457, 1810, 1938
Alw21I GWGCWC 5 cut(s) 810, 1036, 1911, 1940, 1977
Alw44I GTGCAC 1 cut(s) 1907
AlwI GGATC 2 cut(s) 975, 1215
AoxI GGCC 8 cut(s) 38, 255, 406, 800, 1002, 1657, 2069, 2223
ApaLI GTGCAC 1 cut(s) 1907
ApeKI GCWGC 7 cut(s) 383, 935, 1111, 1170, 1230, 1792, 1810
ApoI RAATTY 2 cut(s) 1604, 1636
AseI ATTAAT 1 cut(s) 792
AsiGI ACCGGT 1 cut(s) 2158
AspA2I CCTAGG 1 cut(s) 1912
AspLEI GCGC 2 cut(s) 994, 1143
AspS9I GGNCC 2 cut(s) 166, 406
AsuC2I CCSGG 1 cut(s) 1493
AsuHPI GGTGA 2 cut(s) 678, 1382
AsuII TTCGAA 1 cut(s) 415
AvaII GGWCC 1 cut(s) 166
AvrII CCTAGG 1 cut(s) 1912
AxyI CCTNAGG 1 cut(s) 1578
BaeGI GKGCMC 3 cut(s) 630, 765, 1911
BalI TGGCCA 3 cut(s) 257, 802, 1004
BanII GRGCYC 3 cut(s) 810, 1498, 1940
BauI CACGAG 1 cut(s) 878
Bbv12I GWGCWC 5 cut(s) 810, 1036, 1911, 1940, 1977
BbvI GCAGC 7 cut(s) 370, 922, 1098, 1182, 1242, 1797, 1804
BccI CCATC 5 cut(s) 116, 156, 826, 1220, 2009
BceAI ACGGC 2 cut(s) 288, 2084
BciT130I CCWGG 3 cut(s) 738, 1842, 2007
BclI TGATCA 2 cut(s) 3, 907
BcnI CCSGG 1 cut(s) 1493
BfaI CTAG 2 cut(s) 357, 1913
BfmI CTRYAG 3 cut(s) 384, 1112, 1344
BfoI RGCGCY 1 cut(s) 1144
BfuAI ACCTGC 1 cut(s) 1872
BglI GCCNNNNNGGC 1 cut(s) 37
BisI GCNGC 7 cut(s) 384, 936, 1112, 1171, 1231, 1793, 1811
BlnI CCTAGG 1 cut(s) 1912
BlsI GCNGC 7 cut(s) 385, 937, 1113, 1172, 1232, 1794, 1812
Bme1390I CCNGG 4 cut(s) 738, 1493, 1842, 2007
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 166, 406
BmiI GGNNCC 2 cut(s) 167, 1497
BmrFI CCNGG 4 cut(s) 738, 1493, 1842, 2007
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 6 cut(s) 543, 831, 1316, 1452, 1724, 2215
BmuI ACTGGG 1 cut(s) 576
BpmI CTGGAG 2 cut(s) 137, 681
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 415
BpuMI CCSGG 1 cut(s) 1493
BsaJI CCNNGG 4 cut(s) 270, 737, 1841, 1912
BsaWI WCCGGW 2 cut(s) 2090, 2158
Bsc4I CCNNNNNNNGG 2 cut(s) 1861, 2221
Bse118I RCCGGY 2 cut(s) 40, 2158
Bse1I ACTGG 6 cut(s) 571, 611, 698, 803, 1355, 1511
Bse21I CCTNAGG 1 cut(s) 1578
Bse3DI GCAATG 2 cut(s) 872, 1045
BseBI CCWGG 3 cut(s) 738, 1842, 2007
BseDI CCNNGG 4 cut(s) 270, 737, 1841, 1912
BseGI GGATG 7 cut(s) 108, 453, 558, 1739, 1870, 1922, 1999
BseLI CCNNNNNNNGG 2 cut(s) 1861, 2221
BseMI GCAATG 2 cut(s) 872, 1045
BseMII CTCAG 5 cut(s) 701, 864, 1113, 1592, 1631
BseNI ACTGG 6 cut(s) 571, 611, 698, 803, 1355, 1511
BseRI GAGGAG 1 cut(s) 2194
BseSI GKGCMC 3 cut(s) 630, 765, 1911
BseXI GCAGC 7 cut(s) 370, 922, 1098, 1182, 1242, 1797, 1804
BsgI GTGCAG 1 cut(s) 693
Bsh1236I CGCG 1 cut(s) 562
BshFI GGCC 8 cut(s) 40, 257, 408, 802, 1004, 1659, 2071, 2225
BshTI ACCGGT 1 cut(s) 2158
BsiHKAI GWGCWC 5 cut(s) 810, 1036, 1911, 1940, 1977
BsiSI CCGG 4 cut(s) 41, 1492, 2091, 2159
BslFI GGGAC 1 cut(s) 1911
BslI CCNNNNNNNGG 2 cut(s) 1861, 2221
BsmFI GGGAC 1 cut(s) 1911
BsmI GAATGC 2 cut(s) 241, 1420
BsnI GGCC 8 cut(s) 40, 257, 408, 802, 1004, 1659, 2071, 2225
Bsp119I TTCGAA 1 cut(s) 415
Bsp1286I GDGCHC 8 cut(s) 630, 765, 810, 1036, 1498, 1911, 1940, 1977
Bsp1407I TGTACA 2 cut(s) 322, 1543
Bsp143I GATC 6 cut(s) 3, 123, 907, 967, 997, 1207
BspACI CCGC 2 cut(s) 290, 1016
BspANI GGCC 8 cut(s) 40, 257, 408, 802, 1004, 1659, 2071, 2225
BspCNI CTCAG 5 cut(s) 702, 863, 1114, 1591, 1630
BspFNI CGCG 1 cut(s) 562
BspHI TCATGA 2 cut(s) 1945, 2062
BspLI GGNNCC 2 cut(s) 167, 1497
BspMAI CTGCAG 2 cut(s) 388, 1116
BspMI ACCTGC 1 cut(s) 1872
BspPI GGATC 2 cut(s) 975, 1215
BspT104I TTCGAA 1 cut(s) 415
BsrDI GCAATG 2 cut(s) 872, 1045
BsrFI RCCGGY 2 cut(s) 40, 2158
BsrGI TGTACA 2 cut(s) 322, 1543
BsrI ACTGG 6 cut(s) 571, 611, 698, 803, 1355, 1511
BssAI RCCGGY 2 cut(s) 40, 2158
BssECI CCNNGG 4 cut(s) 270, 737, 1841, 1912
BssMI GATC 6 cut(s) 3, 123, 907, 967, 997, 1207
BssNAI GTATAC 1 cut(s) 2039
BssSI CACGAG 1 cut(s) 878
BssT1I CCWWGG 1 cut(s) 1912
Bst1107I GTATAC 1 cut(s) 2039
Bst2BI CACGAG 1 cut(s) 878
Bst2UI CCWGG 3 cut(s) 738, 1842, 2007
Bst4CI ACNGT 9 cut(s) 23, 177, 198, 253, 625, 671, 857, 1933, 2199
Bst6I CTCTTC 3 cut(s) 531, 942, 1003
BstAPI GCANNNNNTGC 3 cut(s) 1031, 1094, 1734
BstAUI TGTACA 2 cut(s) 322, 1543
BstBI TTCGAA 1 cut(s) 415
BstC8I GCNNGC 6 cut(s) 16, 42, 1036, 1086, 1815, 2003
BstDEI CTNAG 6 cut(s) 276, 710, 850, 1122, 1578, 1617
BstDSI CCRYGG 1 cut(s) 270
BstENI CCTNNNNNAGG 1 cut(s) 2219
BstF5I GGATG 7 cut(s) 108, 453, 558, 1739, 1870, 1922, 1999
BstFNI CGCG 1 cut(s) 562
BstH2I RGCGCY 1 cut(s) 1144
BstHHI GCGC 2 cut(s) 994, 1143
BstKTI GATC 6 cut(s) 6, 126, 910, 970, 1000, 1210
BstMBI GATC 6 cut(s) 3, 123, 907, 967, 997, 1207
BstMWI GCNNNNNNNGC 7 cut(s) 37, 1022, 1031, 1094, 1387, 1463, 1734
BstNI CCWGG 3 cut(s) 738, 1842, 2007
BstNSI RCATGY 7 cut(s) 18, 241, 443, 935, 1088, 1961, 2005
BstSCI CCNGG 4 cut(s) 736, 1491, 1840, 2005
BstSFI CTRYAG 3 cut(s) 384, 1112, 1344
BstSLI GKGCMC 3 cut(s) 630, 765, 1911
BstUI CGCG 1 cut(s) 562
BstV1I GCAGC 7 cut(s) 370, 922, 1098, 1182, 1242, 1797, 1804
BstX2I RGATCY 2 cut(s) 967, 1207
BstXI CCANNNNNNTGG 2 cut(s) 115, 162
BstYI RGATCY 2 cut(s) 967, 1207
BstZ17I GTATAC 1 cut(s) 2039
Bsu36I CCTNAGG 1 cut(s) 1578
BsuRI GGCC 8 cut(s) 40, 257, 408, 802, 1004, 1659, 2071, 2225
BtgI CCRYGG 1 cut(s) 270
BtsCI GGATG 7 cut(s) 108, 453, 558, 1739, 1870, 1922, 1999
BtsIMutI CAGTG 4 cut(s) 705, 862, 1362, 1893
BveI ACCTGC 1 cut(s) 1872
Cac8I GCNNGC 6 cut(s) 16, 42, 1036, 1086, 1815, 2003
CciI TCATGA 2 cut(s) 1945, 2062
CfoI GCGC 2 cut(s) 994, 1143
Cfr10I RCCGGY 2 cut(s) 40, 2158
Cfr13I GGNCC 2 cut(s) 166, 406
CseI GACGC 1 cut(s) 1370
Csp6I GTAC 7 cut(s) 70, 173, 194, 323, 564, 1514, 1544
CspAI ACCGGT 1 cut(s) 2158
CspCI CAANNNNNGTGG 4 cut(s) 234, 269, 1732, 1767
CviQI GTAC 7 cut(s) 70, 173, 194, 323, 564, 1514, 1544
DdeI CTNAG 6 cut(s) 276, 710, 850, 1122, 1578, 1617
DpnI GATC 6 cut(s) 5, 125, 909, 969, 999, 1209
DpnII GATC 6 cut(s) 3, 123, 907, 967, 997, 1207
DraIII CACNNNGTG 1 cut(s) 1981
EaeI YGGCCR 4 cut(s) 38, 255, 800, 1002
Eam1104I CTCTTC 3 cut(s) 531, 942, 1003
EarI CTCTTC 3 cut(s) 531, 942, 1003
Ecl136II GAGCTC 2 cut(s) 808, 1938
Eco130I CCWWGG 1 cut(s) 1912
Eco24I GRGCYC 3 cut(s) 810, 1498, 1940
Eco32I GATATC 1 cut(s) 1585
Eco47I GGWCC 1 cut(s) 166
Eco53kI GAGCTC 2 cut(s) 808, 1938
Eco81I CCTNAGG 1 cut(s) 1578
EcoICRI GAGCTC 2 cut(s) 808, 1938
EcoNI CCTNNNNNAGG 1 cut(s) 2219
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 3 cut(s) 736, 1840, 2005
EcoRV GATATC 1 cut(s) 1585
EcoT14I CCWWGG 1 cut(s) 1912
EcoT22I ATGCAT 2 cut(s) 243, 1086
EcoT38I GRGCYC 3 cut(s) 810, 1498, 1940
ErhI CCWWGG 1 cut(s) 1912
FaqI GGGAC 1 cut(s) 1911
FbaI TGATCA 2 cut(s) 3, 907
FblI GTMKAC 2 cut(s) 436, 2038
Fnu4HI GCNGC 7 cut(s) 384, 936, 1112, 1171, 1231, 1793, 1811
FokI GGATG 7 cut(s) 95, 460, 565, 1746, 1877, 1929, 2006
FriOI GRGCYC 3 cut(s) 810, 1498, 1940
Fsp4HI GCNGC 7 cut(s) 384, 936, 1112, 1171, 1231, 1793, 1811
FspBI CTAG 2 cut(s) 357, 1913
GlaI GCGC 2 cut(s) 993, 1142
GluI GCNGC 7 cut(s) 384, 936, 1112, 1171, 1231, 1793, 1811
GsuI CTGGAG 2 cut(s) 137, 681
HaeII RGCGCY 1 cut(s) 1144
HaeIII GGCC 8 cut(s) 40, 257, 408, 802, 1004, 1659, 2071, 2225
HapII CCGG 4 cut(s) 41, 1492, 2091, 2159
HgaI GACGC 1 cut(s) 1370
HhaI GCGC 2 cut(s) 994, 1143
Hin6I GCGC 2 cut(s) 992, 1141
HinP1I GCGC 2 cut(s) 992, 1141
HincII GTYRAC 3 cut(s) 437, 453, 602
HindII GTYRAC 3 cut(s) 437, 453, 602
HinfI GANTC 8 cut(s) 208, 607, 950, 1475, 1556, 1569, 2145, 2211
HpaII CCGG 4 cut(s) 41, 1492, 2091, 2159
HphI GGTGA 2 cut(s) 678, 1382
Hpy166II GTNNAC 8 cut(s) 437, 453, 602, 877, 1544, 1909, 2039, 2052
Hpy188I TCNGA 7 cut(s) 79, 853, 1277, 1474, 1620, 1763, 2194
Hpy8I GTNNAC 8 cut(s) 437, 453, 602, 877, 1544, 1909, 2039, 2052
HpyAV CCTTC 4 cut(s) 1244, 1430, 1688, 1712
HpyCH4III ACNGT 9 cut(s) 23, 177, 198, 253, 625, 671, 857, 1933, 2199
HpyCH4IV ACGT 3 cut(s) 230, 873, 2059
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 1022, 1031, 1094, 1387, 1463, 1734
HpyF3I CTNAG 6 cut(s) 276, 710, 850, 1122, 1578, 1617
HpySE526I ACGT 3 cut(s) 230, 873, 2059
HspAI GCGC 2 cut(s) 992, 1141
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Ksp22I TGATCA 2 cut(s) 3, 907
Kzo9I GATC 6 cut(s) 3, 123, 907, 967, 997, 1207
LmnI GCTCC 4 cut(s) 979, 1440, 1501, 2181
Lsp1109I GCAGC 7 cut(s) 370, 922, 1098, 1182, 1242, 1797, 1804
LweI GCATC 6 cut(s) 543, 831, 1316, 1452, 1724, 2215
MaeI CTAG 2 cut(s) 357, 1913
MaeII ACGT 3 cut(s) 230, 873, 2059
MaeIII GTNAC 3 cut(s) 927, 1888, 1924
MalI GATC 6 cut(s) 5, 125, 909, 969, 999, 1209
MboI GATC 6 cut(s) 3, 123, 907, 967, 997, 1207
MboII GAAGA 7 cut(s) 385, 548, 629, 959, 1020, 1095, 1235
MflI RGATCY 2 cut(s) 967, 1207
MhlI GDGCHC 8 cut(s) 630, 765, 810, 1036, 1498, 1911, 1940, 1977
MlsI TGGCCA 3 cut(s) 257, 802, 1004
MluI ACGCGT 1 cut(s) 560
MluNI TGGCCA 3 cut(s) 257, 802, 1004
MlyI GAGTC 4 cut(s) 202, 959, 2154, 2220
Mox20I TGGCCA 3 cut(s) 257, 802, 1004
Mph1103I ATGCAT 2 cut(s) 243, 1086
MroNI GCCGGC 1 cut(s) 40
MscI TGGCCA 3 cut(s) 257, 802, 1004
MseI TTAA 9 cut(s) 284, 351, 495, 792, 1163, 1257, 1292, 1296, 1788
MslI CAYNNNNRTG 2 cut(s) 160, 263
Msp20I TGGCCA 3 cut(s) 257, 802, 1004
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 4 cut(s) 41, 1492, 2091, 2159
MspR9I CCNGG 4 cut(s) 738, 1493, 1842, 2007
Mva1269I GAATGC 2 cut(s) 241, 1420
MvaI CCWGG 3 cut(s) 738, 1842, 2007
MvnI CGCG 1 cut(s) 562
MwoI GCNNNNNNNGC 7 cut(s) 37, 1022, 1031, 1094, 1387, 1463, 1734
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 1493
NdeII GATC 6 cut(s) 3, 123, 907, 967, 997, 1207
NgoMIV GCCGGC 1 cut(s) 40
NlaIV GGNNCC 2 cut(s) 167, 1497
NmuCI GTSAC 2 cut(s) 927, 1888
NsiI ATGCAT 2 cut(s) 243, 1086
NspI RCATGY 7 cut(s) 18, 241, 443, 935, 1088, 1961, 2005
NspV TTCGAA 1 cut(s) 415
PacI TTAATTAA 1 cut(s) 1296
PaeI GCATGC 3 cut(s) 18, 1088, 2005
PagI TCATGA 2 cut(s) 1945, 2062
PciI ACATGT 1 cut(s) 439
PctI GAATGC 2 cut(s) 241, 1420
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 4 cut(s) 607, 1475, 1556, 1569
PflFI GACNNNGTC 1 cut(s) 2144
PinAI ACCGGT 1 cut(s) 2158
PkrI GCNGC 7 cut(s) 385, 937, 1113, 1172, 1232, 1794, 1812
PleI GAGTC 4 cut(s) 202, 958, 2153, 2219
PpsI GAGTC 4 cut(s) 202, 958, 2153, 2219
PpuMI RGGWCCY 1 cut(s) 166
PscI ACATGT 1 cut(s) 439
PshBI ATTAAT 1 cut(s) 792
PsiI TTATAA 1 cut(s) 1193
Psp124BI GAGCTC 2 cut(s) 810, 1940
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 3 cut(s) 736, 1840, 2005
PspGI CCWGG 3 cut(s) 736, 1840, 2005
PspN4I GGNNCC 2 cut(s) 167, 1497
PspPI GGNCC 2 cut(s) 166, 406
PspPPI RGGWCCY 1 cut(s) 166
PstI CTGCAG 2 cut(s) 388, 1116
PsuI RGATCY 2 cut(s) 967, 1207
PsyI GACNNNGTC 1 cut(s) 2144
RsaI GTAC 7 cut(s) 71, 174, 195, 324, 565, 1515, 1545
RsaNI GTAC 7 cut(s) 70, 173, 194, 323, 564, 1514, 1544
RseI CAYNNNNRTG 2 cut(s) 160, 263
SacI GAGCTC 2 cut(s) 810, 1940
SalI GTCGAC 1 cut(s) 435
SaqAI TTAA 9 cut(s) 284, 351, 495, 792, 1163, 1257, 1292, 1296, 1788
SatI GCNGC 7 cut(s) 384, 936, 1112, 1171, 1231, 1793, 1811
Sau3AI GATC 6 cut(s) 3, 123, 907, 967, 997, 1207
Sau96I GGNCC 2 cut(s) 166, 406
SchI GAGTC 4 cut(s) 202, 959, 2154, 2220
ScrFI CCNGG 4 cut(s) 738, 1493, 1842, 2007
SduI GDGCHC 8 cut(s) 630, 765, 810, 1036, 1498, 1911, 1940, 1977
SfaNI GCATC 6 cut(s) 543, 831, 1316, 1452, 1724, 2215
SfcI CTRYAG 3 cut(s) 384, 1112, 1344
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 2 cut(s) 160, 263
SphI GCATGC 3 cut(s) 18, 1088, 2005
SsiI CCGC 2 cut(s) 290, 1016
SspI AATATT 2 cut(s) 942, 988
SspMI CTAG 2 cut(s) 357, 1913
SstI GAGCTC 2 cut(s) 810, 1940
StyD4I CCNGG 4 cut(s) 736, 1491, 1840, 2005
StyI CCWWGG 1 cut(s) 1912
TaaI ACNGT 9 cut(s) 23, 177, 198, 253, 625, 671, 857, 1933, 2199
TaiI ACGT 3 cut(s) 233, 876, 2062
TaqI TCGA 6 cut(s) 415, 436, 510, 1221, 2209, 2220
TatI WGTACW 5 cut(s) 69, 193, 322, 1513, 1543
TfiI GAWTC 4 cut(s) 607, 1475, 1556, 1569
Tru1I TTAA 9 cut(s) 284, 351, 495, 792, 1163, 1257, 1292, 1296, 1788
Tru9I TTAA 9 cut(s) 284, 351, 495, 792, 1163, 1257, 1292, 1296, 1788
TscAI CASTG 4 cut(s) 705, 862, 1362, 1893
TseFI GTSAC 2 cut(s) 927, 1888
TseI GCWGC 7 cut(s) 383, 935, 1111, 1170, 1230, 1792, 1810
Tsp45I GTSAC 2 cut(s) 927, 1888
TspGWI ACGGA 2 cut(s) 587, 2157
TspRI CASTG 4 cut(s) 705, 862, 1362, 1893
Tth111I GACNNNGTC 1 cut(s) 2144
VneI GTGCAC 1 cut(s) 1907
VpaK11BI GGWCC 1 cut(s) 166
VspI ATTAAT 1 cut(s) 792
XagI CCTNNNNNAGG 1 cut(s) 2219
XapI RAATTY 2 cut(s) 1604, 1636
XceI RCATGY 7 cut(s) 18, 241, 443, 935, 1088, 1961, 2005
XmaJI CCTAGG 1 cut(s) 1912
XmiI GTMKAC 2 cut(s) 436, 2038
XspI CTAG 2 cut(s) 357, 1913
Zsp2I ATGCAT 2 cut(s) 243, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.