Rh7BG373900

May act early in the ethylene signal transduction pathway, possibly as an ethylene receptor, or as a regulator of the pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0003094
Physical Location & Seq
Reverse (-)
40226520 .. 40232159
5640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG373900.1

Sequence Viewer

Length: 2226 bp
ATGATGGAGGGGTGCTGCATTGAGCCACAATGGCCGGCTGATGAATTGTTGATGAAGTATCAGTACATTTCAGATTTCTTTATTGCACTTGCTTATTTTTCCATCCCTCTGGAGTTAATCTACTTTGTCAAGAAATCTGCCGTGTTTCCATACAAATGGGTTCTTGTTCAGTTTGGTGCTTTTATTGTATTGTGTGGAGCGACACATCTGATTAACTTATGGACTTTTAGCGTGCATTCAAGAACTGTGGCCATAGTAATGGCTACTGCCAAGGTTTTCACTGCTGCGGTGTCATGTGCTACTGCCCTCATGCTTGTTCATATTATTCCTGATTTACTGAGTGTTAAAACTAGAGAGTTATTCCTGAAGAACAAGGCTGCAGAGCTCGACAGAGAAATGGGTTTGATTCGTACACAGGAAGAAACAGGTAGACATGTCAGGATGCTGACGCATGAGATCAGAAGCACTCTTGATCGACATACTATACTGAAAACCACACTTGTTGAATTAGGTAGAATTTTGACATTGGAAGAGTGTGCCTTGTGGATGCCAACACGCACTGGGTTGGAGCTTCAACTATCCTATACTCTTCATCAGCAGAATCCAGTAGGATACACTGTACCCATCCATCTTCCTGTGATCAATCAAGTATTTAACAACAACGGTACAGTAAAAATATCACCGAATTCTCCAGTGGCCAGACTAAGGCCTATTGCTGGAAAACACATTCCTGGGGAGGTTGTTGCAGTTCGTGTCCCACTCCTACATCTCTCTAACTTCCAAATTAATGACTGGCCAGAGCTCTCAACAAAACGCTATGCTTTGATGGTGTTGATGCTTCCCTCTGATAGTGCTAGGCAATGGCATGTCCATGAGTTAGAGCTGGTGGAGGTAGTTGCTGATCAGGTGGCAGTTGCTCTTTCACATGCTGCAATATTAGAAGAGTCAAGGAAGGCCAGGGATCTTCTTATGGAGCAGAATATTGCACTTGATTTGGCCAGGAGGGATGCTGAAATTGCAATTTGTGCTCGCAATGATTTCTTGGCTGTTATGAACCATGAGATGAGAACTCCCATGCATGCAATTATTGCCCTTTCTTCCTTGCTGCAAGAAACTGAACTGACACCTGAGCAGCGCCTGATGGTTGAAACAATACTAAAGAGCAGTAACCTTTTGGCTACCCTCATTAATGATGTATTAGATCTTTCAAGGCTTGAAGATGGCAGCCTTCAACTCGATATAGCAACTTTTAACCTCCATTCTTTATTCAGAGAGGTGCACAGTTTAGTTAAGCCTGTTGCCTCTGTCAAAAAGTTGTCGGTTACATTAAATTTAGCAGCAGATTTGCCAGTATATGCGGTAGGTGATGAAAAGCGCCTTATGCAAACTATTCTAAATGTTGTCGGGAATGCTGTGAAGTTCACAAAAGAAGGGACCATCTCGATCGCAGTTTTTGTTGCCAAATCAGAATCCTTAAGAGATTTCTGGGCTCCTGATTTTTTTCCAGTGCAGAGTGATAATCACTTTTATCTGCGGGTACAGGTAAAAGATTCAGGATCTGGAATTAATCCCCAGGATATTCCAAAGCTTTTCACCAAATTCGCACAAACTCAGTCATTAGCTACTAGAAATTCTGGTGGTAGTGGACTTGGCCTTGCAATTTGTAAGAGATTTGTAAATCTTATGGAGGGGCACATTTGGATTGAAAGTGATGGTCTGGGCAAGGGATGTACTGCCACTGTCATTGTGAAACTCGGATATTCAGAACAGTCGAATGAATCTAAGCTGCCTTTTGCGCCTAAAACGCAAGCAAATCATGTACATTCAGACTTTCCTGGGTTAAAAGTTCTTGTCATGGATGATAACGGGGTCAGCAGGTCAGCGACAGAGGGACTCCTTGTGCACCTGGGATGTGATGTAACATCTGTAAGCTCAAGTGAGGAGTTCTTGCGTGTTATATCTCAGGAGCACAAGGTTGTGTTCATGGATGTATGCATGCCAAGCATAGATGGTTTTGAACTCACTGTCCAGATTCATGAAAAATTTATTAAGCGTCATGAACGGCCAGTATTAGTCGCACTCACTGGTAACATGGACAGGATGACAAAGGAGAGCTGCATGAGGGTTGGTATGGATGGAGTTATACTAAAACCTATGTCATTAGATAAGATGAGGAGCGTTTTGTCAGAACTATTAGAGCATCGAGTTCTATTTGAAGCCACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009308 GO:0009408 GO:0009593 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009651 GO:0009690 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009733 GO:0009737 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010087 GO:0010104 GO:0010105 GO:0010119 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016999 GO:0017000 GO:0017144 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0023051 GO:0023052 GO:0023057 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0033993 GO:0034754 GO:0035556 GO:0036211 GO:0038023 GO:0038199 GO:0042221 GO:0042445 GO:0042545 GO:0042562 GO:0042742 GO:0042743 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046483 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048856 GO:0050665 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051239 GO:0051301 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0051740 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0072328 GO:0072593 GO:0097305 GO:0098542 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1903409 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
GAF HATPase_c HisKA Response_reg

Protein Analysis

741

Amino Acids

82.86

Weight (kDa)

6.37

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETR1_N PF25487 17 - 115 4.6e-38 Ethylene receptor 1-like, N-terminal domain
GAF PF01590 158 - 307 2e-11 GAF domain
HisKA PF00512 343 - 408 3.2e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 455 - 585 1e-24 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 616 - 727 3.2e-17 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1866
AccI GTMKAC 1 cut(s) 430
AciI CCGC 3 cut(s) 287, 1358, 1534
AclWI GGATC 2 cut(s) 969, 1564
AcoI YGGCCR 6 cut(s) 32, 249, 696, 794, 996, 2063
AcsI RAATTY 6 cut(s) 516, 685, 1330, 1598, 1630, 2042
AcuI CTGAAG 1 cut(s) 386
AfaI GTAC 7 cut(s) 65, 412, 621, 667, 1539, 1732, 1821
AfiI CCNNNNNNNGG 2 cut(s) 705, 716
AflII CTTAAG 1 cut(s) 1474
AflIII ACRYGT 1 cut(s) 433
AjnI CCWGG 6 cut(s) 730, 956, 998, 1572, 1834, 1905
AloI GAACNNNNNNTCC 2 cut(s) 2010, 2042
AluBI AGCT 9 cut(s) 385, 571, 802, 883, 1588, 1622, 1786, 1932, 2115
AluI AGCT 9 cut(s) 385, 571, 802, 883, 1588, 1622, 1786, 1932, 2115
Alw21I GWGCWC 6 cut(s) 387, 804, 1030, 1281, 1905, 1971
Alw44I GTGCAC 2 cut(s) 1277, 1901
AlwI GGATC 2 cut(s) 969, 1564
AlwNI CAGNNNCTG 2 cut(s) 1138, 1559
AoxI GGCC 9 cut(s) 32, 249, 696, 707, 794, 954, 996, 1651, 2063
ApaLI GTGCAC 2 cut(s) 1277, 1901
ApoI RAATTY 6 cut(s) 516, 685, 1330, 1598, 1630, 2042
AseI ATTAAT 3 cut(s) 786, 1188, 1566
AspLEI GCGC 3 cut(s) 1137, 1377, 1798
AspS9I GGNCC 1 cut(s) 1434
AsuHPI GGTGA 3 cut(s) 672, 1376, 1585
AvaII GGWCC 1 cut(s) 1434
BaeGI GKGCMC 3 cut(s) 1281, 1695, 1905
BalI TGGCCA 4 cut(s) 251, 698, 796, 998
BanII GRGCYC 3 cut(s) 387, 804, 1492
BarI GAAGNNNNNNTAC 2 cut(s) 47, 79
Bbv12I GWGCWC 6 cut(s) 387, 804, 1030, 1281, 1905, 1971
BceAI ACGGC 2 cut(s) 125, 2078
BciT130I CCWGG 6 cut(s) 732, 958, 1000, 1574, 1836, 1907
BciVI GTATCC 1 cut(s) 605
BclI TGATCA 2 cut(s) 639, 901
BfaI CTAG 3 cut(s) 351, 855, 1626
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 2 cut(s) 1138, 1378
BfrI CTTAAG 1 cut(s) 1474
BfuAI ACCTGC 1 cut(s) 1866
BfuI GTATCC 1 cut(s) 605
BglI GCCNNNNNGGC 1 cut(s) 31
BglII AGATCT 1 cut(s) 1201
Bme1390I CCNGG 6 cut(s) 732, 958, 1000, 1574, 1836, 1907
Bme18I GGWCC 1 cut(s) 1434
BmgT120I GGNCC 1 cut(s) 1434
BmiI GGNNCC 2 cut(s) 1435, 1491
BmrFI CCNGG 6 cut(s) 732, 958, 1000, 1574, 1836, 1907
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 5 cut(s) 432, 537, 825, 997, 2209
BmuI ACTGGG 1 cut(s) 570
BpmI CTGGAG 2 cut(s) 131, 675
Bpu10I CCTNAGC 1 cut(s) 1128
BpuEI CTTGAG 1 cut(s) 1918
BsaAI YACGTR 1 cut(s) 2223
BsaJI CCNNGG 6 cut(s) 270, 731, 957, 1572, 1835, 1906
Bsc4I CCNNNNNNNGG 2 cut(s) 705, 716
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 8 cut(s) 565, 605, 692, 797, 1349, 1505, 2066, 2089
Bse3DI GCAATG 2 cut(s) 866, 1039
BseBI CCWGG 6 cut(s) 732, 958, 1000, 1574, 1836, 1907
BseDI CCNNGG 6 cut(s) 270, 731, 957, 1572, 1835, 1906
BseLI CCNNNNNNNGG 2 cut(s) 705, 716
BseMI GCAATG 2 cut(s) 866, 1039
BseMII CTCAG 4 cut(s) 329, 1119, 1625, 1976
BseNI ACTGG 8 cut(s) 565, 605, 692, 797, 1349, 1505, 2066, 2089
BseRI GAGGAG 2 cut(s) 1955, 2188
BseSI GKGCMC 3 cut(s) 1281, 1695, 1905
BsgI GTGCAG 1 cut(s) 1529
Bsh1285I CGRYCG 1 cut(s) 1446
BshFI GGCC 9 cut(s) 34, 251, 698, 709, 796, 956, 998, 1653, 2065
BsiEI CGRYCG 1 cut(s) 1446
BsiHKAI GWGCWC 6 cut(s) 387, 804, 1030, 1281, 1905, 1971
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 3 cut(s) 740, 1447, 1905
BslI CCNNNNNNNGG 2 cut(s) 705, 716
BsmFI GGGAC 3 cut(s) 740, 1447, 1905
BsmI GAATGC 2 cut(s) 235, 1414
BsnI GGCC 9 cut(s) 34, 251, 698, 709, 796, 956, 998, 1653, 2065
Bsp1286I GDGCHC 8 cut(s) 387, 804, 1030, 1281, 1492, 1695, 1905, 1971
Bsp1407I TGTACA 1 cut(s) 1819
Bsp143I GATC 8 cut(s) 456, 472, 639, 901, 961, 1201, 1443, 1556
BspACI CCGC 3 cut(s) 287, 1358, 1534
BspANI GGCC 9 cut(s) 34, 251, 698, 709, 796, 956, 998, 1653, 2065
BspCNI CTCAG 4 cut(s) 330, 1120, 1624, 1975
BspHI TCATGA 2 cut(s) 2035, 2056
BspLI GGNNCC 2 cut(s) 1435, 1491
BspMAI CTGCAG 1 cut(s) 382
BspMI ACCTGC 1 cut(s) 1866
BspPI GGATC 2 cut(s) 969, 1564
BspTI CTTAAG 1 cut(s) 1474
BsrDI GCAATG 2 cut(s) 866, 1039
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 1819
BsrI ACTGG 8 cut(s) 565, 605, 692, 797, 1349, 1505, 2066, 2089
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 6 cut(s) 270, 731, 957, 1572, 1835, 1906
BssMI GATC 8 cut(s) 456, 472, 639, 901, 961, 1201, 1443, 1556
BssT1I CCWWGG 1 cut(s) 270
Bst2UI CCWGG 6 cut(s) 732, 958, 1000, 1574, 1836, 1907
Bst4CI ACNGT 8 cut(s) 247, 619, 665, 670, 1283, 1741, 1770, 2026
Bst6I CTCTTC 3 cut(s) 525, 594, 936
BstAFI CTTAAG 1 cut(s) 1474
BstAPI GCANNNNNTGC 2 cut(s) 1025, 1088
BstAUI TGTACA 1 cut(s) 1819
BstBAI YACGTR 1 cut(s) 2223
BstC8I GCNNGC 6 cut(s) 36, 233, 1030, 1080, 1809, 1997
BstDEI CTNAG 6 cut(s) 338, 704, 1128, 1611, 1782, 1962
BstH2I RGCGCY 2 cut(s) 1138, 1378
BstHHI GCGC 3 cut(s) 1137, 1377, 1798
BstKTI GATC 8 cut(s) 459, 475, 642, 904, 964, 1204, 1446, 1559
BstMBI GATC 8 cut(s) 456, 472, 639, 901, 961, 1201, 1443, 1556
BstMCI CGRYCG 1 cut(s) 1446
BstMWI GCNNNNNNNGC 8 cut(s) 31, 1016, 1025, 1088, 1381, 1795, 1804, 2001
BstNI CCWGG 6 cut(s) 732, 958, 1000, 1574, 1836, 1907
BstNSI RCATGY 5 cut(s) 437, 869, 929, 1082, 1999
BstSCI CCNGG 6 cut(s) 730, 956, 998, 1572, 1834, 1905
BstSFI CTRYAG 1 cut(s) 378
BstSLI GKGCMC 3 cut(s) 1281, 1695, 1905
BstX2I RGATCY 3 cut(s) 961, 1201, 1556
BstXI CCANNNNNNTGG 3 cut(s) 109, 156, 259
BstYI RGATCY 3 cut(s) 961, 1201, 1556
BsuI GTATCC 1 cut(s) 605
BsuRI GGCC 9 cut(s) 34, 251, 698, 709, 796, 956, 998, 1653, 2065
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 8 cut(s) 279, 558, 615, 699, 1512, 1737, 2022, 2082
BveI ACCTGC 1 cut(s) 1866
Cac8I GCNNGC 6 cut(s) 36, 233, 1030, 1080, 1809, 1997
CaiI CAGNNNCTG 2 cut(s) 1138, 1559
CciI TCATGA 2 cut(s) 2035, 2056
CfoI GCGC 3 cut(s) 1137, 1377, 1798
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 1434
CseI GACGC 2 cut(s) 457, 2042
Csp6I GTAC 7 cut(s) 64, 411, 620, 666, 1538, 1731, 1820
CspCI CAANNNNNGTGG 6 cut(s) 228, 263, 484, 519, 1726, 1761
CviQI GTAC 7 cut(s) 64, 411, 620, 666, 1538, 1731, 1820
DdeI CTNAG 6 cut(s) 338, 704, 1128, 1611, 1782, 1962
DpnI GATC 8 cut(s) 458, 474, 641, 903, 963, 1203, 1445, 1558
DpnII GATC 8 cut(s) 456, 472, 639, 901, 961, 1201, 1443, 1556
EaeI YGGCCR 6 cut(s) 32, 249, 696, 794, 996, 2063
Eam1104I CTCTTC 3 cut(s) 525, 594, 936
EarI CTCTTC 3 cut(s) 525, 594, 936
Ecl136II GAGCTC 2 cut(s) 385, 802
Eco130I CCWWGG 1 cut(s) 270
Eco147I AGGCCT 1 cut(s) 709
Eco24I GRGCYC 3 cut(s) 387, 804, 1492
Eco47I GGWCC 1 cut(s) 1434
Eco53kI GAGCTC 2 cut(s) 385, 802
Eco57I CTGAAG 1 cut(s) 386
EcoICRI GAGCTC 2 cut(s) 385, 802
EcoRI GAATTC 1 cut(s) 685
EcoRII CCWGG 6 cut(s) 730, 956, 998, 1572, 1834, 1905
EcoT14I CCWWGG 1 cut(s) 270
EcoT22I ATGCAT 2 cut(s) 1080, 1997
EcoT38I GRGCYC 3 cut(s) 387, 804, 1492
ErhI CCWWGG 1 cut(s) 270
FaqI GGGAC 3 cut(s) 740, 1447, 1905
FauI CCCGC 1 cut(s) 1527
FbaI TGATCA 2 cut(s) 639, 901
FblI GTMKAC 1 cut(s) 430
FriOI GRGCYC 3 cut(s) 387, 804, 1492
FspBI CTAG 3 cut(s) 351, 855, 1626
GlaI GCGC 3 cut(s) 1136, 1376, 1797
GsuI CTGGAG 2 cut(s) 131, 675
HaeII RGCGCY 2 cut(s) 1138, 1378
HaeIII GGCC 9 cut(s) 34, 251, 698, 709, 796, 956, 998, 1653, 2065
HapII CCGG 1 cut(s) 35
HgaI GACGC 2 cut(s) 457, 2042
HhaI GCGC 3 cut(s) 1137, 1377, 1798
Hin6I GCGC 3 cut(s) 1135, 1375, 1796
HinP1I GCGC 3 cut(s) 1135, 1375, 1796
HindIII AAGCTT 1 cut(s) 1586
HinfI GANTC 8 cut(s) 406, 601, 944, 1469, 1550, 1778, 1893, 2032
HpaII CCGG 1 cut(s) 35
HphI GGTGA 3 cut(s) 672, 1376, 1585
Hpy166II GTNNAC 6 cut(s) 413, 431, 1279, 1422, 1646, 1903
Hpy8I GTNNAC 6 cut(s) 413, 431, 1279, 1422, 1646, 1903
HpyAV CCTTC 3 cut(s) 946, 1238, 1424
HpyCH4III ACNGT 8 cut(s) 247, 619, 665, 670, 1283, 1741, 1770, 2026
HpyCH4IV ACGT 1 cut(s) 2222
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 1016, 1025, 1088, 1381, 1795, 1804, 2001
HpyF3I CTNAG 6 cut(s) 338, 704, 1128, 1611, 1782, 1962
HpySE526I ACGT 1 cut(s) 2222
HspAI GCGC 3 cut(s) 1135, 1375, 1796
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Ksp22I TGATCA 2 cut(s) 639, 901
Kzo9I GATC 8 cut(s) 456, 472, 639, 901, 961, 1201, 1443, 1556
LmnI GCTCC 6 cut(s) 197, 568, 973, 1495, 1966, 2175
LweI GCATC 5 cut(s) 432, 537, 825, 997, 2209
MaeI CTAG 3 cut(s) 351, 855, 1626
MaeII ACGT 1 cut(s) 2222
MaeIII GTNAC 4 cut(s) 1166, 1321, 1918, 2087
MalI GATC 8 cut(s) 458, 474, 641, 903, 963, 1203, 1445, 1558
MboI GATC 8 cut(s) 456, 472, 639, 901, 961, 1201, 1443, 1556
MboII GAAGA 9 cut(s) 379, 431, 542, 581, 623, 953, 956, 1089, 1229
MflI RGATCY 3 cut(s) 961, 1201, 1556
MhlI GDGCHC 8 cut(s) 387, 804, 1030, 1281, 1492, 1695, 1905, 1971
MlsI TGGCCA 4 cut(s) 251, 698, 796, 998
MluNI TGGCCA 4 cut(s) 251, 698, 796, 998
MlyI GAGTC 2 cut(s) 953, 1887
MmeI TCCRAC 1 cut(s) 546
Mox20I TGGCCA 4 cut(s) 251, 698, 796, 998
Mph1103I ATGCAT 2 cut(s) 1080, 1997
MroNI GCCGGC 1 cut(s) 34
MscI TGGCCA 4 cut(s) 251, 698, 796, 998
MslI CAYNNNNRTG 3 cut(s) 154, 257, 870
Msp20I TGGCCA 4 cut(s) 251, 698, 796, 998
MspCI CTTAAG 1 cut(s) 1474
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 6 cut(s) 732, 958, 1000, 1574, 1836, 1907
Mva1269I GAATGC 2 cut(s) 235, 1414
MvaI CCWGG 6 cut(s) 732, 958, 1000, 1574, 1836, 1907
MwoI GCNNNNNNNGC 8 cut(s) 31, 1016, 1025, 1088, 1381, 1795, 1804, 2001
NaeI GCCGGC 1 cut(s) 36
NdeII GATC 8 cut(s) 456, 472, 639, 901, 961, 1201, 1443, 1556
NgoMIV GCCGGC 1 cut(s) 34
NlaIV GGNNCC 2 cut(s) 1435, 1491
NsiI ATGCAT 2 cut(s) 1080, 1997
NspI RCATGY 5 cut(s) 437, 869, 929, 1082, 1999
PaeI GCATGC 2 cut(s) 1082, 1999
PagI TCATGA 2 cut(s) 2035, 2056
PceI AGGCCT 1 cut(s) 709
PciI ACATGT 1 cut(s) 433
PctI GAATGC 2 cut(s) 235, 1414
PdiI GCCGGC 1 cut(s) 36
PfeI GAWTC 6 cut(s) 406, 601, 1469, 1550, 1778, 2032
Ple19I CGATCG 1 cut(s) 1446
PleI GAGTC 2 cut(s) 952, 1887
PpsI GAGTC 2 cut(s) 952, 1887
Ppu21I YACGTR 1 cut(s) 2223
PscI ACATGT 1 cut(s) 433
PshBI ATTAAT 3 cut(s) 786, 1188, 1566
Psp124BI GAGCTC 2 cut(s) 387, 804
Psp6I CCWGG 6 cut(s) 730, 956, 998, 1572, 1834, 1905
PspGI CCWGG 6 cut(s) 730, 956, 998, 1572, 1834, 1905
PspN4I GGNNCC 2 cut(s) 1435, 1491
PspPI GGNCC 1 cut(s) 1434
PstI CTGCAG 1 cut(s) 382
PstNI CAGNNNCTG 2 cut(s) 1138, 1559
PsuI RGATCY 3 cut(s) 961, 1201, 1556
PvuI CGATCG 1 cut(s) 1446
RsaI GTAC 7 cut(s) 65, 412, 621, 667, 1539, 1732, 1821
RsaNI GTAC 7 cut(s) 64, 411, 620, 666, 1538, 1731, 1820
RseI CAYNNNNRTG 3 cut(s) 154, 257, 870
SacI GAGCTC 2 cut(s) 387, 804
Sau3AI GATC 8 cut(s) 456, 472, 639, 901, 961, 1201, 1443, 1556
Sau96I GGNCC 1 cut(s) 1434
SchI GAGTC 2 cut(s) 953, 1887
ScrFI CCNGG 6 cut(s) 732, 958, 1000, 1574, 1836, 1907
SduI GDGCHC 8 cut(s) 387, 804, 1030, 1281, 1492, 1695, 1905, 1971
SfaNI GCATC 5 cut(s) 432, 537, 825, 997, 2209
SfcI CTRYAG 1 cut(s) 378
SinI GGWCC 1 cut(s) 1434
SmiMI CAYNNNNRTG 3 cut(s) 154, 257, 870
SmlI CTYRAG 2 cut(s) 1474, 1933
SmoI CTYRAG 2 cut(s) 1474, 1933
SphI GCATGC 2 cut(s) 1082, 1999
SseBI AGGCCT 1 cut(s) 709
SsiI CCGC 3 cut(s) 287, 1358, 1534
SspI AATATT 2 cut(s) 936, 982
SspMI CTAG 3 cut(s) 351, 855, 1626
SstI GAGCTC 2 cut(s) 387, 804
StuI AGGCCT 1 cut(s) 709
StyD4I CCNGG 6 cut(s) 730, 956, 998, 1572, 1834, 1905
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 8 cut(s) 247, 619, 665, 670, 1283, 1741, 1770, 2026
TaiI ACGT 1 cut(s) 2225
TaqI TCGA 6 cut(s) 387, 475, 1236, 1442, 1772, 2203
TatI WGTACW 3 cut(s) 63, 1730, 1819
TfiI GAWTC 6 cut(s) 406, 601, 1469, 1550, 1778, 2032
TscAI CASTG 8 cut(s) 286, 565, 622, 699, 1512, 1744, 2029, 2089
TspRI CASTG 8 cut(s) 286, 565, 622, 699, 1512, 1744, 2029, 2089
Vha464I CTTAAG 1 cut(s) 1474
VneI GTGCAC 2 cut(s) 1277, 1901
VpaK11BI GGWCC 1 cut(s) 1434
VspI ATTAAT 3 cut(s) 786, 1188, 1566
XapI RAATTY 6 cut(s) 516, 685, 1330, 1598, 1630, 2042
XceI RCATGY 5 cut(s) 437, 869, 929, 1082, 1999
XmiI GTMKAC 1 cut(s) 430
XspI CTAG 3 cut(s) 351, 855, 1626
Zsp2I ATGCAT 2 cut(s) 1080, 1997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.