pycom02g12760

May act early in the ethylene signal transduction pathway, possibly as an ethylene receptor, or as a regulator of the pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003094
Physical Location & Seq
Forward (+)
9316771 .. 9321625
4855 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12760.2

Sequence Viewer

Length: 2232 bp
ATGATCATGGAGGCATGCAACTGTATTGAGCCACAATGGCCGGCAGATGAATTGCTGATGAAATATCAGTACATTTCGGATTTCTTTATTGCGCTTGCTTATTTCTCCATCCCTCTGGAGTTGATCTACTTTGTCAAGAAATCTGCAGTGTTTCCATATAGATGGGTCCTCGTACAGTTTGGTGCTTTCATAGTACTGTGTGGGGCGACTCATCTTATCAACTTATGGACGTTCAACATGCATTCAAGAACTGTGGCCATAGTAATGACCACAGCTAAGGTTTTAACTGCGGTGGTGTCATGTGCAACTGCCCTTATGCTTGTACATATTATACCTGATCTATTGAGTGTTAAAACTAGAGAACTATTCTTGAAGAACAAGGCTGCAGAGCTCGATAGAGAAATGGGTCTTATTCGAACACAGGAGGAAACAGGTCGACATGTCAGGATGTTGACCCATGAAATCAGAAGCACTCTTGATAGACATACTATACTTAAAACTACACTTGTCGAGTTAGGTAGAACTCTGGCATTGGAAGAGTGTGCCTTGTGGATGCCGACGCGTACTGGGTTAGAGCTTCAACTATCCTATACACTCCATCAGCAGAATCCAGTAGGATATACTGTGCCCATTCATCTTCCTGTGGTCAATCAAGTATTTAGTAGCAACTGTGCAGTAAAAATATCACCTAATTCTCCAGTGGCACGACTGAGGCAACTTGCAGGAAAGCACATTCCTGGGGAAGTTGTTGCAGTTCGTGTGCCCCTTTTACATCTCTCTAACTTCCAAATTAATGACTGGCCAGAGCTCTCAACAAAACGCTATGCTTTGATGGTCTTGATGCTTCCCTCAGACAGTGCAAGGCAATGGCATGTTCACGAGTTAGAATTGGTTGAAGTAGTTGCTGATCAGGTTGCAGTTGCTCTGTCACATGCTGCAATATTAGAAGAGTCAATGAGGGCAAGGGATCTCCTTATGGAGCAGAATATTGCGCTTGATCTGGCCAGTAGAGAAGCGGAAACTGCAATTCGTGCTCGCAATGATTTCTTGGCTGTTATGAACCATGAGATGAGAACTCCCATGCATGCAATTATTGCTCTTTCTTCTTTGCTGCAGGAAACTGATCTGACACCTGAGCAGCGCCTCATGGTTGAAACGATATTAAAGAGCAGCAACCTATTGGCTACCCTTATAAATGATGTTTTGGATCTTTCAAGGCTCGAAGATGGCAGCCTTCAACTTGACATAGCAACTTTTAACCTCCATTCCATATTCAGAGAGGTTCATAATTTAATTAAGCCTGTTGCATCTGTCAAAAAGTTGTCGGTTGCATTGAATATAGCTGCAGATTTGCCAGTGTATGCCATAGGTGATGAAAAGCGTCTTATGCAAATTATTCTAAATGTTGTTGGGAATGCTGTGAAGTTCTCAAAAGAAGGGAGCATCTCAATCACAGCTTATGTTGCAAAATCAGAATCTTTACGAGATTTCCGGGCTCCTGATTTCTTTCCAGTGCAGAGTGATAATCACTTCTATCTGCGTGTACAGGTCAAAGATTCAGGATCAGGAATCAATCCTCAGGATATTCCAAATCTATTTACCAAATTCACTCAAACTCAGACTTTAGCTTCTCAAAATTCTGGTGGTAGTGGGCTTGGCCTTGCAATTTGTAAGAGATTTGTAAATCTTATGGAAGGGCATATTTGGATTGAAAGTGAAGGTCTTGGCAAGGGATGCACAGCCACTTTCATTGTGAAACTCGGCTTTCCAGAGCGTTCAAATGAGTCTAAGCAGCCTTTTGCACCCAAGCTGCAAGCAAATCACGTTCAAACAAACTTTCCAGGGTTGAAAGTCCTTGTTATGGATGATAATGGGGTCAGCAGGTCAGTGACAAAGGGACTTCTTGTGCACCTAGGATGTGATGTAACAACTGTGAGCTCAATTGATGAGTTCTTGCACGTAATTTCGCAAGAGCACAAGGTGGTGTTCATGGATGTGTGCATGCCAGGTATAGATGGCTATGAACTTGCTGTTCGTATACATGAAAGGTTCACAAAACGCCATGAACGGCCTGTATTAGTAGCACTTACCGGAAGCATAGACAAGATGACAAAAGAGAACTGCATGAGGGTTGGTATGGATGGAGTCATACTAAAACCGGTTTCAGTTGATAAAATGAGGAGTGTTTTATCGGAACTGTTAGAGCATCGAGTCCTATTCGAGGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009308 GO:0009408 GO:0009593 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009651 GO:0009690 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009733 GO:0009737 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010087 GO:0010104 GO:0010105 GO:0010119 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016999 GO:0017000 GO:0017144 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0023051 GO:0023052 GO:0023057 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0033993 GO:0034754 GO:0035556 GO:0036211 GO:0038023 GO:0038199 GO:0042221 GO:0042445 GO:0042545 GO:0042562 GO:0042742 GO:0042743 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046483 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048856 GO:0050665 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051239 GO:0051301 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0051740 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0072328 GO:0072593 GO:0097305 GO:0098542 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1903409 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
GAF HATPase_c HisKA Response_reg

Protein Analysis

744

Amino Acids

83.19

Weight (kDa)

6.52

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1193
Acc36I ACCTGC 1 cut(s) 1872
AccI GTMKAC 2 cut(s) 436, 2038
AccII CGCG 1 cut(s) 562
AciI CCGC 2 cut(s) 290, 1016
AclWI GGATC 3 cut(s) 975, 1215, 1570
AcoI YGGCCR 4 cut(s) 38, 255, 800, 1002
AcsI RAATTY 2 cut(s) 1604, 1636
AdeI CACNNNGTG 1 cut(s) 1981
AfaI GTAC 6 cut(s) 71, 174, 195, 324, 565, 1545
AfiI CCNNNNNNNGG 2 cut(s) 1861, 2221
AflIII ACRYGT 2 cut(s) 439, 560
AgeI ACCGGT 1 cut(s) 2158
AjnI CCWGG 3 cut(s) 736, 1840, 2005
AluBI AGCT 9 cut(s) 275, 391, 577, 808, 1343, 1457, 1628, 1810, 1938
AluI AGCT 9 cut(s) 275, 391, 577, 808, 1343, 1457, 1628, 1810, 1938
Alw21I GWGCWC 6 cut(s) 393, 810, 1036, 1911, 1940, 1977
Alw44I GTGCAC 1 cut(s) 1907
AlwI GGATC 3 cut(s) 975, 1215, 1570
AoxI GGCC 7 cut(s) 38, 255, 800, 1002, 1657, 2069, 2223
ApaLI GTGCAC 1 cut(s) 1907
ApeKI GCWGC 9 cut(s) 383, 935, 1111, 1138, 1170, 1230, 1343, 1792, 1810
ApoI RAATTY 2 cut(s) 1604, 1636
AseI ATTAAT 1 cut(s) 792
AsiGI ACCGGT 1 cut(s) 2158
AspA2I CCTAGG 1 cut(s) 1912
AspLEI GCGC 3 cut(s) 94, 994, 1143
AspS9I GGNCC 1 cut(s) 166
AsuC2I CCSGG 1 cut(s) 1493
AsuHPI GGTGA 2 cut(s) 678, 1382
AsuII TTCGAA 1 cut(s) 415
AvaII GGWCC 1 cut(s) 166
AvrII CCTAGG 1 cut(s) 1912
AxyI CCTNAGG 1 cut(s) 1578
BaeGI GKGCMC 3 cut(s) 630, 765, 1911
BalI TGGCCA 3 cut(s) 257, 802, 1004
BanII GRGCYC 4 cut(s) 393, 810, 1498, 1940
BauI CACGAG 1 cut(s) 878
Bbv12I GWGCWC 6 cut(s) 393, 810, 1036, 1911, 1940, 1977
BbvI GCAGC 9 cut(s) 370, 922, 1098, 1150, 1182, 1242, 1330, 1797, 1804
BccI CCATC 7 cut(s) 116, 156, 606, 826, 1220, 2009, 2135
BceAI ACGGC 1 cut(s) 2084
BciT130I CCWGG 3 cut(s) 738, 1842, 2007
BclI TGATCA 2 cut(s) 3, 907
BcnI CCSGG 1 cut(s) 1493
BfaI CTAG 2 cut(s) 357, 1913
BfmI CTRYAG 4 cut(s) 144, 384, 1112, 1344
BfoI RGCGCY 1 cut(s) 1144
BfuAI ACCTGC 1 cut(s) 1872
BglI GCCNNNNNGGC 1 cut(s) 37
BisI GCNGC 9 cut(s) 384, 936, 1112, 1139, 1171, 1231, 1344, 1793, 1811
BlnI CCTAGG 1 cut(s) 1912
BlsI GCNGC 9 cut(s) 385, 937, 1113, 1140, 1172, 1232, 1345, 1794, 1812
BmcAI AGTACT 1 cut(s) 195
Bme1390I CCNGG 4 cut(s) 738, 1493, 1842, 2007
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 2 cut(s) 167, 1497
BmrFI CCNGG 4 cut(s) 738, 1493, 1842, 2007
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 6 cut(s) 543, 831, 1316, 1452, 1724, 2215
BmuI ACTGGG 1 cut(s) 576
BpmI CTGGAG 2 cut(s) 137, 681
Bpu10I CCTNAGC 2 cut(s) 276, 1134
Bpu14I TTCGAA 1 cut(s) 415
BpuMI CCSGG 1 cut(s) 1493
BsaAI YACGTR 1 cut(s) 1960
BsaJI CCNNGG 3 cut(s) 737, 1841, 1912
BsaWI WCCGGW 2 cut(s) 2090, 2158
Bsc4I CCNNNNNNNGG 2 cut(s) 1861, 2221
Bse118I RCCGGY 2 cut(s) 40, 2158
Bse1I ACTGG 7 cut(s) 571, 611, 698, 803, 1005, 1355, 1511
Bse21I CCTNAGG 1 cut(s) 1578
Bse3DI GCAATG 2 cut(s) 872, 1045
BseBI CCWGG 3 cut(s) 738, 1842, 2007
BseDI CCNNGG 3 cut(s) 737, 1841, 1912
BseGI GGATG 8 cut(s) 108, 453, 558, 1739, 1870, 1922, 1999, 2146
BseLI CCNNNNNNNGG 2 cut(s) 1861, 2221
BseMI GCAATG 2 cut(s) 872, 1045
BseMII CTCAG 5 cut(s) 701, 864, 1125, 1592, 1631
BseNI ACTGG 7 cut(s) 571, 611, 698, 803, 1005, 1355, 1511
BseRI GAGGAG 1 cut(s) 2194
BseSI GKGCMC 3 cut(s) 630, 765, 1911
BseXI GCAGC 9 cut(s) 370, 922, 1098, 1150, 1182, 1242, 1330, 1797, 1804
BsgI GTGCAG 2 cut(s) 693, 1535
Bsh1236I CGCG 1 cut(s) 562
BshFI GGCC 7 cut(s) 40, 257, 802, 1004, 1659, 2071, 2225
BshTI ACCGGT 1 cut(s) 2158
BsiHKAI GWGCWC 6 cut(s) 393, 810, 1036, 1911, 1940, 1977
BsiSI CCGG 4 cut(s) 41, 1492, 2091, 2159
BslFI GGGAC 1 cut(s) 1911
BslI CCNNNNNNNGG 2 cut(s) 1861, 2221
BsmFI GGGAC 1 cut(s) 1911
BsmI GAATGC 2 cut(s) 241, 1420
BsnI GGCC 7 cut(s) 40, 257, 802, 1004, 1659, 2071, 2225
Bsp119I TTCGAA 1 cut(s) 415
Bsp1286I GDGCHC 9 cut(s) 393, 630, 765, 810, 1036, 1498, 1911, 1940, 1977
Bsp1407I TGTACA 2 cut(s) 322, 1543
Bsp143I GATC 9 cut(s) 3, 123, 337, 907, 967, 997, 1123, 1207, 1562
BspACI CCGC 2 cut(s) 290, 1016
BspANI GGCC 7 cut(s) 40, 257, 802, 1004, 1659, 2071, 2225
BspCNI CTCAG 5 cut(s) 702, 863, 1126, 1591, 1630
BspFNI CGCG 1 cut(s) 562
BspLI GGNNCC 2 cut(s) 167, 1497
BspMAI CTGCAG 4 cut(s) 148, 388, 1116, 1348
BspMI ACCTGC 1 cut(s) 1872
BspPI GGATC 3 cut(s) 975, 1215, 1570
BspT104I TTCGAA 1 cut(s) 415
BsrDI GCAATG 2 cut(s) 872, 1045
BsrFI RCCGGY 2 cut(s) 40, 2158
BsrGI TGTACA 2 cut(s) 322, 1543
BsrI ACTGG 7 cut(s) 571, 611, 698, 803, 1005, 1355, 1511
BssAI RCCGGY 2 cut(s) 40, 2158
BssECI CCNNGG 3 cut(s) 737, 1841, 1912
BssMI GATC 9 cut(s) 3, 123, 337, 907, 967, 997, 1123, 1207, 1562
BssNAI GTATAC 1 cut(s) 2039
BssSI CACGAG 1 cut(s) 878
BssT1I CCWWGG 1 cut(s) 1912
Bst1107I GTATAC 1 cut(s) 2039
Bst2BI CACGAG 1 cut(s) 878
Bst2UI CCWGG 3 cut(s) 738, 1842, 2007
Bst4CI ACNGT 9 cut(s) 23, 177, 198, 253, 625, 671, 857, 1933, 2199
Bst6I CTCTTC 2 cut(s) 531, 942
BstAPI GCANNNNNTGC 3 cut(s) 1031, 1094, 1734
BstAUI TGTACA 2 cut(s) 322, 1543
BstBAI YACGTR 1 cut(s) 1960
BstBI TTCGAA 1 cut(s) 415
BstC8I GCNNGC 7 cut(s) 16, 42, 96, 1036, 1086, 1815, 2003
BstDEI CTNAG 7 cut(s) 276, 710, 850, 1134, 1578, 1617, 1788
BstENI CCTNNNNNAGG 1 cut(s) 2219
BstF5I GGATG 8 cut(s) 108, 453, 558, 1739, 1870, 1922, 1999, 2146
BstFNI CGCG 1 cut(s) 562
BstH2I RGCGCY 1 cut(s) 1144
BstHHI GCGC 3 cut(s) 94, 994, 1143
BstKTI GATC 9 cut(s) 6, 126, 340, 910, 970, 1000, 1126, 1210, 1565
BstMBI GATC 9 cut(s) 3, 123, 337, 907, 967, 997, 1123, 1207, 1562
BstMWI GCNNNNNNNGC 7 cut(s) 37, 1022, 1031, 1094, 1387, 1463, 1734
BstNI CCWGG 3 cut(s) 738, 1842, 2007
BstNSI RCATGY 7 cut(s) 18, 241, 443, 875, 935, 1088, 2005
BstSCI CCNGG 4 cut(s) 736, 1491, 1840, 2005
BstSFI CTRYAG 4 cut(s) 144, 384, 1112, 1344
BstSLI GKGCMC 3 cut(s) 630, 765, 1911
BstUI CGCG 1 cut(s) 562
BstV1I GCAGC 9 cut(s) 370, 922, 1098, 1150, 1182, 1242, 1330, 1797, 1804
BstX2I RGATCY 2 cut(s) 967, 1207
BstXI CCANNNNNNTGG 2 cut(s) 115, 162
BstYI RGATCY 2 cut(s) 967, 1207
BstZ17I GTATAC 1 cut(s) 2039
Bsu36I CCTNAGG 1 cut(s) 1578
BsuRI GGCC 7 cut(s) 40, 257, 802, 1004, 1659, 2071, 2225
BtsCI GGATG 8 cut(s) 108, 453, 558, 1739, 1870, 1922, 1999, 2146
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 6 cut(s) 153, 705, 862, 1362, 1518, 1893
BveI ACCTGC 1 cut(s) 1872
Cac8I GCNNGC 7 cut(s) 16, 42, 96, 1036, 1086, 1815, 2003
CfoI GCGC 3 cut(s) 94, 994, 1143
Cfr10I RCCGGY 2 cut(s) 40, 2158
Cfr13I GGNCC 1 cut(s) 166
CseI GACGC 2 cut(s) 568, 1370
Csp6I GTAC 6 cut(s) 70, 173, 194, 323, 564, 1544
CspAI ACCGGT 1 cut(s) 2158
CspCI CAANNNNNGTGG 4 cut(s) 234, 269, 1732, 1767
CviQI GTAC 6 cut(s) 70, 173, 194, 323, 564, 1544
DdeI CTNAG 7 cut(s) 276, 710, 850, 1134, 1578, 1617, 1788
DpnI GATC 9 cut(s) 5, 125, 339, 909, 969, 999, 1125, 1209, 1564
DpnII GATC 9 cut(s) 3, 123, 337, 907, 967, 997, 1123, 1207, 1562
DraIII CACNNNGTG 1 cut(s) 1981
EaeI YGGCCR 4 cut(s) 38, 255, 800, 1002
Eam1104I CTCTTC 2 cut(s) 531, 942
EarI CTCTTC 2 cut(s) 531, 942
Ecl136II GAGCTC 3 cut(s) 391, 808, 1938
Eco130I CCWWGG 1 cut(s) 1912
Eco24I GRGCYC 4 cut(s) 393, 810, 1498, 1940
Eco47I GGWCC 1 cut(s) 166
Eco53kI GAGCTC 3 cut(s) 391, 808, 1938
Eco81I CCTNAGG 1 cut(s) 1578
EcoICRI GAGCTC 3 cut(s) 391, 808, 1938
EcoNI CCTNNNNNAGG 1 cut(s) 2219
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 3 cut(s) 736, 1840, 2005
EcoT14I CCWWGG 1 cut(s) 1912
EcoT22I ATGCAT 2 cut(s) 243, 1086
EcoT38I GRGCYC 4 cut(s) 393, 810, 1498, 1940
ErhI CCWWGG 1 cut(s) 1912
FaqI GGGAC 1 cut(s) 1911
FbaI TGATCA 2 cut(s) 3, 907
FblI GTMKAC 2 cut(s) 436, 2038
Fnu4HI GCNGC 9 cut(s) 384, 936, 1112, 1139, 1171, 1231, 1344, 1793, 1811
FokI GGATG 8 cut(s) 95, 460, 565, 1746, 1877, 1929, 2006, 2153
FriOI GRGCYC 4 cut(s) 393, 810, 1498, 1940
Fsp4HI GCNGC 9 cut(s) 384, 936, 1112, 1139, 1171, 1231, 1344, 1793, 1811
FspBI CTAG 2 cut(s) 357, 1913
GlaI GCGC 3 cut(s) 93, 993, 1142
GluI GCNGC 9 cut(s) 384, 936, 1112, 1139, 1171, 1231, 1344, 1793, 1811
GsuI CTGGAG 2 cut(s) 137, 681
HaeII RGCGCY 1 cut(s) 1144
HaeIII GGCC 7 cut(s) 40, 257, 802, 1004, 1659, 2071, 2225
HapII CCGG 4 cut(s) 41, 1492, 2091, 2159
HgaI GACGC 2 cut(s) 568, 1370
HhaI GCGC 3 cut(s) 94, 994, 1143
Hin6I GCGC 3 cut(s) 92, 992, 1141
HinP1I GCGC 3 cut(s) 92, 992, 1141
HincII GTYRAC 2 cut(s) 437, 453
HindII GTYRAC 2 cut(s) 437, 453
HinfI GANTC 9 cut(s) 208, 607, 950, 1475, 1556, 1569, 1784, 2145, 2211
HpaII CCGG 4 cut(s) 41, 1492, 2091, 2159
HphI GGTGA 2 cut(s) 678, 1382
Hpy166II GTNNAC 7 cut(s) 437, 453, 877, 1544, 1909, 2039, 2052
Hpy188I TCNGA 8 cut(s) 79, 467, 853, 1128, 1277, 1474, 1620, 2194
Hpy8I GTNNAC 7 cut(s) 437, 453, 877, 1544, 1909, 2039, 2052
Hpy99I CGWCG 1 cut(s) 562
HpyAV CCTTC 4 cut(s) 1244, 1430, 1688, 1712
HpyCH4III ACNGT 9 cut(s) 23, 177, 198, 253, 625, 671, 857, 1933, 2199
HpyCH4IV ACGT 3 cut(s) 230, 1824, 1959
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 1022, 1031, 1094, 1387, 1463, 1734
HpyF3I CTNAG 7 cut(s) 276, 710, 850, 1134, 1578, 1617, 1788
HpySE526I ACGT 3 cut(s) 230, 1824, 1959
HspAI GCGC 3 cut(s) 92, 992, 1141
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Ksp22I TGATCA 2 cut(s) 3, 907
Kzo9I GATC 9 cut(s) 3, 123, 337, 907, 967, 997, 1123, 1207, 1562
LmnI GCTCC 3 cut(s) 979, 1440, 1501
Lsp1109I GCAGC 9 cut(s) 370, 922, 1098, 1150, 1182, 1242, 1330, 1797, 1804
LweI GCATC 6 cut(s) 543, 831, 1316, 1452, 1724, 2215
MaeI CTAG 2 cut(s) 357, 1913
MaeII ACGT 3 cut(s) 230, 1824, 1959
MaeIII GTNAC 3 cut(s) 927, 1888, 1924
MalI GATC 9 cut(s) 5, 125, 339, 909, 969, 999, 1125, 1209, 1564
MboI GATC 9 cut(s) 3, 123, 337, 907, 967, 997, 1123, 1207, 1562
MboII GAAGA 6 cut(s) 385, 548, 629, 959, 1095, 1235
MfeI CAATTG 1 cut(s) 1941
MflI RGATCY 2 cut(s) 967, 1207
MhlI GDGCHC 9 cut(s) 393, 630, 765, 810, 1036, 1498, 1911, 1940, 1977
MlsI TGGCCA 3 cut(s) 257, 802, 1004
MluI ACGCGT 1 cut(s) 560
MluNI TGGCCA 3 cut(s) 257, 802, 1004
MlyI GAGTC 5 cut(s) 202, 959, 1793, 2154, 2220
Mox20I TGGCCA 3 cut(s) 257, 802, 1004
Mph1103I ATGCAT 2 cut(s) 243, 1086
MroNI GCCGGC 1 cut(s) 40
MscI TGGCCA 3 cut(s) 257, 802, 1004
MseI TTAA 8 cut(s) 284, 351, 495, 792, 1163, 1257, 1292, 1296
MslI CAYNNNNRTG 3 cut(s) 160, 263, 1994
Msp20I TGGCCA 3 cut(s) 257, 802, 1004
MspI CCGG 4 cut(s) 41, 1492, 2091, 2159
MspR9I CCNGG 4 cut(s) 738, 1493, 1842, 2007
MunI CAATTG 1 cut(s) 1941
Mva1269I GAATGC 2 cut(s) 241, 1420
MvaI CCWGG 3 cut(s) 738, 1842, 2007
MvnI CGCG 1 cut(s) 562
MwoI GCNNNNNNNGC 7 cut(s) 37, 1022, 1031, 1094, 1387, 1463, 1734
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 1493
NdeII GATC 9 cut(s) 3, 123, 337, 907, 967, 997, 1123, 1207, 1562
NgoMIV GCCGGC 1 cut(s) 40
NlaIV GGNNCC 2 cut(s) 167, 1497
NmeAIII GCCGAG 1 cut(s) 1740
NmuCI GTSAC 2 cut(s) 927, 1888
NsiI ATGCAT 2 cut(s) 243, 1086
NspI RCATGY 7 cut(s) 18, 241, 443, 875, 935, 1088, 2005
NspV TTCGAA 1 cut(s) 415
PacI TTAATTAA 1 cut(s) 1296
PaeI GCATGC 3 cut(s) 18, 1088, 2005
PciI ACATGT 1 cut(s) 439
PctI GAATGC 2 cut(s) 241, 1420
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 4 cut(s) 607, 1475, 1556, 1569
PinAI ACCGGT 1 cut(s) 2158
PkrI GCNGC 9 cut(s) 385, 937, 1113, 1140, 1172, 1232, 1345, 1794, 1812
PleI GAGTC 5 cut(s) 202, 958, 1792, 2153, 2219
PpsI GAGTC 5 cut(s) 202, 958, 1792, 2153, 2219
Ppu21I YACGTR 1 cut(s) 1960
PpuMI RGGWCCY 1 cut(s) 166
PscI ACATGT 1 cut(s) 439
PshBI ATTAAT 1 cut(s) 792
PsiI TTATAA 1 cut(s) 1193
Psp124BI GAGCTC 3 cut(s) 393, 810, 1940
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 3 cut(s) 736, 1840, 2005
PspGI CCWGG 3 cut(s) 736, 1840, 2005
PspN4I GGNNCC 2 cut(s) 167, 1497
PspPI GGNCC 1 cut(s) 166
PspPPI RGGWCCY 1 cut(s) 166
PstI CTGCAG 4 cut(s) 148, 388, 1116, 1348
PsuI RGATCY 2 cut(s) 967, 1207
RsaI GTAC 6 cut(s) 71, 174, 195, 324, 565, 1545
RsaNI GTAC 6 cut(s) 70, 173, 194, 323, 564, 1544
RseI CAYNNNNRTG 3 cut(s) 160, 263, 1994
SacI GAGCTC 3 cut(s) 393, 810, 1940
SalI GTCGAC 1 cut(s) 435
SaqAI TTAA 8 cut(s) 284, 351, 495, 792, 1163, 1257, 1292, 1296
SatI GCNGC 9 cut(s) 384, 936, 1112, 1139, 1171, 1231, 1344, 1793, 1811
Sau3AI GATC 9 cut(s) 3, 123, 337, 907, 967, 997, 1123, 1207, 1562
Sau96I GGNCC 1 cut(s) 166
ScaI AGTACT 1 cut(s) 195
SchI GAGTC 5 cut(s) 202, 959, 1793, 2154, 2220
ScrFI CCNGG 4 cut(s) 738, 1493, 1842, 2007
SduI GDGCHC 9 cut(s) 393, 630, 765, 810, 1036, 1498, 1911, 1940, 1977
SfaNI GCATC 6 cut(s) 543, 831, 1316, 1452, 1724, 2215
SfcI CTRYAG 4 cut(s) 144, 384, 1112, 1344
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 3 cut(s) 160, 263, 1994
SphI GCATGC 3 cut(s) 18, 1088, 2005
SsiI CCGC 2 cut(s) 290, 1016
SspI AATATT 2 cut(s) 942, 988
SspMI CTAG 2 cut(s) 357, 1913
SstI GAGCTC 3 cut(s) 393, 810, 1940
StyD4I CCNGG 4 cut(s) 736, 1491, 1840, 2005
StyI CCWWGG 1 cut(s) 1912
TaaI ACNGT 9 cut(s) 23, 177, 198, 253, 625, 671, 857, 1933, 2199
TaiI ACGT 3 cut(s) 233, 1827, 1962
TaqI TCGA 7 cut(s) 393, 415, 436, 510, 1221, 2209, 2220
TatI WGTACW 4 cut(s) 69, 193, 322, 1543
TfiI GAWTC 4 cut(s) 607, 1475, 1556, 1569
Tru1I TTAA 8 cut(s) 284, 351, 495, 792, 1163, 1257, 1292, 1296
Tru9I TTAA 8 cut(s) 284, 351, 495, 792, 1163, 1257, 1292, 1296
TscAI CASTG 6 cut(s) 153, 705, 862, 1362, 1518, 1893
TseFI GTSAC 2 cut(s) 927, 1888
TseI GCWGC 9 cut(s) 383, 935, 1111, 1138, 1170, 1230, 1343, 1792, 1810
Tsp45I GTSAC 2 cut(s) 927, 1888
TspRI CASTG 6 cut(s) 153, 705, 862, 1362, 1518, 1893
VneI GTGCAC 1 cut(s) 1907
VpaK11BI GGWCC 1 cut(s) 166
VspI ATTAAT 1 cut(s) 792
XagI CCTNNNNNAGG 1 cut(s) 2219
XapI RAATTY 2 cut(s) 1604, 1636
XceI RCATGY 7 cut(s) 18, 241, 443, 875, 935, 1088, 2005
XmaJI CCTAGG 1 cut(s) 1912
XmiI GTMKAC 2 cut(s) 436, 2038
XspI CTAG 2 cut(s) 357, 1913
ZrmI AGTACT 1 cut(s) 195
Zsp2I ATGCAT 2 cut(s) 243, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.