AT1G66520

Formyl transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009304
Physical Location & Seq
Reverse (-)
24816128 .. 24818744
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66520.2

Sequence Viewer

Length: 1068 bp
ATGATACTACGTCGTTTCATCTGCTACAACGCTTCTTCAACTGTTTCATCTATAGCTCCATCACCGAAGAAGAAGCCTCTCATCTTCTTAGGCTCTCCTCAGGTCTCCGTGAGTGTGCTTGAAGCTCTTTTCAATGCATCTAATGCTCCAAACTCTTCCTTCGAGGTTGCAGGTATTGTTACACAGCCTCCATCAAGGAGAGATAGGGGTAAAAAAGTGTTGCCTTCACCAGTAGCGCAATACGCTCTTGATAAAGGCTTACCTTCTGATCTCATTTTCTCCCCTGAGAAGGCAGGAGATGAAGCATTTTTATCGGCTTTAAGAGAGTTGCAACCTGAGCTTTGTATTACAGCAGCTTATGGGAATATTTTGCCTACTAAGTTCCTTAAGATTCCGGTACATGGGACAGTGAACATACACCCAAGTTTGCTGCCGCTGTACCGTGGTGCAGCTCCAGTTCAAAGAGCATTACAGGATGGTGTCCCGGAAACAGGAGTATCATTAGCATTTACTGTGCGTAAGTTAGATGCAGGGCCAGTGATTGCCTCTAAGAGGTTCCAAGTGGATGATCTAATAAAGGCACCAGAACTACTCTCGTTCCTATTTTCTGAAGGTTCTAATCTTCTTATCCGTGAACTTCCCTCGATATTTGATGGGTCCGCAAAATCAAAAGCAGCTCCCCAAGATGATTCTAAAGCTACCTTAGCTCCAAAGATAGCTCCAGATGAGGCTTGGCTCTCTTTTGACCAGGAAGCTTTTGTTCTACATAACAAGGTTCGTGCATTTGCAGGATGGCCGGGAACACGAGCAAAAGTTGTAGTCCTTGATGAGAAAAGCGGTCAGCAAAATGTGCTAGAGCTTAAAATTATGTCCACTCGAGTATGCAAAGATCTGGAAATTCAGGATAGTGAACAAGATTATGTAACTTTCAAGAAAGGTTCACTAATATTTCCCTGCAGAGGAGGTACAGCTTTAGAGGTACTGGAAGTCCAGCTTCCTGGTAAGAAAGCCATCAACGCAGCTGCTTTTTGGAATGGCTTGAGAGGTCAAAAGCTGAAGAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko00970 map00670 map00970
Pfam Domains
Protein Families
Formyl_trans_C Formyl_trans_N

Protein Analysis

355

Amino Acids

38.5

Weight (kDa)

9.29

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Formyl_trans_N PF00551 37 - 190 1e-33 Formyl transferase
Formyl_trans_C PF02911 238 - 327 6.3e-08 Formyl transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 580
AciI CCGC 3 cut(s) 434, 660, 837
AcoI YGGCCR 1 cut(s) 794
AcsI RAATTY 1 cut(s) 897
AcuI CTGAAG 1 cut(s) 630
AfaI GTAC 4 cut(s) 399, 440, 967, 981
AfiI CCNNNNNNNGG 5 cut(s) 289, 401, 491, 552, 959
AflII CTTAAG 1 cut(s) 386
AgsI TTSAA 5 cut(s) 39, 122, 133, 461, 931
AjnI CCWGG 2 cut(s) 747, 997
Alw26I GTCTC 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 876
AoxI GGCC 2 cut(s) 533, 794
ApeKI GCWGC 6 cut(s) 353, 430, 449, 674, 1019, 1022
ApoI RAATTY 1 cut(s) 897
AspLEI GCGC 1 cut(s) 238
AspS9I GGNCC 2 cut(s) 533, 657
AsuC2I CCSGG 2 cut(s) 485, 798
AsuHPI GGTGA 2 cut(s) 54, 219
AvaI CYCGRG 1 cut(s) 876
AvaII GGWCC 1 cut(s) 657
AxyI CCTNAGG 1 cut(s) 99
BaeI ACNNNNGTAYC 2 cut(s) 480, 513
BanI GGYRCC 1 cut(s) 580
BauI CACGAG 1 cut(s) 804
BbvI GCAGC 6 cut(s) 365, 417, 461, 686, 1009, 1031
BccI CCATC 6 cut(s) 67, 199, 470, 647, 786, 1019
BcgI CGANNNNNNTGC 2 cut(s) 294, 328
BciT130I CCWGG 2 cut(s) 749, 999
BcnI CCSGG 2 cut(s) 485, 798
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 1 cut(s) 854
BfmI CTRYAG 2 cut(s) 51, 955
BfrI CTTAAG 1 cut(s) 386
BfuAI ACCTGC 1 cut(s) 161
BglII AGATCT 1 cut(s) 889
BisI GCNGC 7 cut(s) 354, 431, 434, 450, 675, 1020, 1023
BlsI GCNGC 7 cut(s) 355, 432, 435, 451, 676, 1021, 1024
Bme1390I CCNGG 4 cut(s) 485, 749, 798, 999
Bme18I GGWCC 1 cut(s) 657
BmeT110I CYCGRG 1 cut(s) 876
BmgT120I GGNCC 2 cut(s) 533, 657
BmiI GGNNCC 3 cut(s) 557, 582, 658
BmrFI CCNGG 4 cut(s) 485, 749, 798, 999
BmsI GCATC 2 cut(s) 146, 517
BpmI CTGGAG 2 cut(s) 438, 705
Bpu10I CCTNAGC 2 cut(s) 336, 703
BpuEI CTTGAG 1 cut(s) 1060
BpuMI CCSGG 2 cut(s) 485, 798
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 1 cut(s) 442
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 4 cut(s) 172, 202, 691, 721
Bsc4I CCNNNNNNNGG 5 cut(s) 289, 401, 491, 552, 959
Bse1I ACTGG 4 cut(s) 230, 455, 536, 987
Bse21I CCTNAGG 1 cut(s) 99
BseBI CCWGG 2 cut(s) 749, 999
BseDI CCNNGG 1 cut(s) 442
BseGI GGATG 3 cut(s) 481, 571, 797
BseLI CCNNNNNNNGG 5 cut(s) 289, 401, 491, 552, 959
BseMII CTCAG 3 cut(s) 113, 276, 327
BseNI ACTGG 4 cut(s) 230, 455, 536, 987
BseRI GAGGAG 2 cut(s) 87, 975
BseXI GCAGC 6 cut(s) 365, 417, 461, 686, 1009, 1031
BsgI GTGCAG 1 cut(s) 468
BshFI GGCC 2 cut(s) 535, 796
BshNI GGYRCC 1 cut(s) 580
BsiHKCI CYCGRG 1 cut(s) 876
BsiSI CCGG 3 cut(s) 395, 485, 797
BslFI GGGAC 2 cut(s) 418, 467
BslI CCNNNNNNNGG 5 cut(s) 289, 401, 491, 552, 959
BsmAI GTCTC 1 cut(s) 109
BsmFI GGGAC 2 cut(s) 418, 467
BsnI GGCC 2 cut(s) 535, 796
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 876
Bsp143I GATC 3 cut(s) 268, 568, 889
BspACI CCGC 3 cut(s) 434, 660, 837
BspANI GGCC 2 cut(s) 535, 796
BspCNI CTCAG 3 cut(s) 112, 277, 328
BspLI GGNNCC 3 cut(s) 557, 582, 658
BspMAI CTGCAG 1 cut(s) 959
BspMI ACCTGC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 580
BspTI CTTAAG 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 109
BsrI ACTGG 4 cut(s) 230, 455, 536, 987
BssECI CCNNGG 1 cut(s) 442
BssMI GATC 3 cut(s) 268, 568, 889
BssSI CACGAG 1 cut(s) 804
Bst2BI CACGAG 1 cut(s) 804
Bst2UI CCWGG 2 cut(s) 749, 999
Bst4CI ACNGT 4 cut(s) 43, 409, 443, 514
Bst6I CTCTTC 1 cut(s) 160
BstAFI CTTAAG 1 cut(s) 386
BstAPI GCANNNNNTGC 2 cut(s) 143, 850
BstDEI CTNAG 7 cut(s) 88, 99, 285, 336, 378, 549, 703
BstDSI CCRYGG 1 cut(s) 442
BstENI CCTNNNNNAGG 1 cut(s) 550
BstF5I GGATG 3 cut(s) 481, 571, 797
BstHHI GCGC 1 cut(s) 238
BstKTI GATC 3 cut(s) 271, 571, 892
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 3 cut(s) 268, 568, 889
BstMWI GCNNNNNNNGC 7 cut(s) 143, 242, 337, 704, 850, 1016, 1060
BstNI CCWGG 2 cut(s) 749, 999
BstSCI CCNGG 4 cut(s) 483, 747, 796, 997
BstSFI CTRYAG 2 cut(s) 51, 955
BstV1I GCAGC 6 cut(s) 365, 417, 461, 686, 1009, 1031
BstX2I RGATCY 1 cut(s) 889
BstXI CCANNNNNNTGG 1 cut(s) 998
BstYI RGATCY 1 cut(s) 889
Bsu36I CCTNAGG 1 cut(s) 99
BsuRI GGCC 2 cut(s) 535, 796
BtgI CCRYGG 1 cut(s) 442
BtsCI GGATG 3 cut(s) 481, 571, 797
BtsIMutI CAGTG 2 cut(s) 414, 543
BveI ACCTGC 1 cut(s) 161
CfoI GCGC 1 cut(s) 238
Cfr13I GGNCC 2 cut(s) 533, 657
Csp6I GTAC 4 cut(s) 398, 439, 966, 980
CviAII CATG 1 cut(s) 401
CviQI GTAC 4 cut(s) 398, 439, 966, 980
DdeI CTNAG 7 cut(s) 88, 99, 285, 336, 378, 549, 703
DpnI GATC 3 cut(s) 270, 570, 891
DpnII GATC 3 cut(s) 268, 568, 889
EaeI YGGCCR 1 cut(s) 794
Eam1104I CTCTTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 657
Eco57I CTGAAG 1 cut(s) 630
Eco81I CCTNAGG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 876
EcoNI CCTNNNNNAGG 1 cut(s) 550
EcoRII CCWGG 2 cut(s) 747, 997
EcoT22I ATGCAT 1 cut(s) 139
FaeI CATG 1 cut(s) 404
FaiI YATR 9 cut(s) 53, 360, 402, 416, 768, 869, 883, 921, 1066
FalI AAGNNNNNCTT 2 cut(s) 978, 1010
FaqI GGGAC 2 cut(s) 418, 467
FatI CATG 1 cut(s) 400
Fnu4HI GCNGC 7 cut(s) 354, 431, 434, 450, 675, 1020, 1023
FokI GGATG 3 cut(s) 488, 578, 804
Fsp4HI GCNGC 7 cut(s) 354, 431, 434, 450, 675, 1020, 1023
FspBI CTAG 1 cut(s) 854
GlaI GCGC 1 cut(s) 237
GluI GCNGC 7 cut(s) 354, 431, 434, 450, 675, 1020, 1023
GsuI CTGGAG 2 cut(s) 438, 705
HaeIII GGCC 2 cut(s) 535, 796
HapII CCGG 3 cut(s) 395, 485, 797
HhaI GCGC 1 cut(s) 238
Hin1II CATG 1 cut(s) 404
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HindIII AAGCTT 1 cut(s) 753
HinfI GANTC 2 cut(s) 391, 689
HpaII CCGG 3 cut(s) 395, 485, 797
HphI GGTGA 2 cut(s) 54, 219
Hpy166II GTNNAC 5 cut(s) 412, 635, 873, 911, 941
Hpy188I TCNGA 2 cut(s) 268, 610
Hpy188III TCNNGA 5 cut(s) 248, 722, 893, 902, 931
Hpy8I GTNNAC 5 cut(s) 412, 635, 873, 911, 941
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 5 cut(s) 169, 234, 273, 283, 605
HpyCH4III ACNGT 4 cut(s) 43, 409, 443, 514
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 9 cut(s) 137, 170, 331, 449, 530, 782, 788, 885, 957
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 242, 337, 704, 850, 1016, 1060
HpyF3I CTNAG 7 cut(s) 88, 99, 285, 336, 378, 549, 703
HpySE526I ACGT 1 cut(s) 10
Hsp92II CATG 1 cut(s) 404
HspAI GCGC 1 cut(s) 236
Kzo9I GATC 3 cut(s) 268, 568, 889
LmnI GCTCC 6 cut(s) 61, 151, 457, 682, 712, 724
Lsp1109I GCAGC 6 cut(s) 365, 417, 461, 686, 1009, 1031
LweI GCATC 2 cut(s) 146, 517
MaeI CTAG 1 cut(s) 854
MaeII ACGT 1 cut(s) 10
MaeIII GTNAC 2 cut(s) 178, 922
MalI GATC 3 cut(s) 270, 570, 891
MboI GATC 3 cut(s) 268, 568, 889
MboII GAAGA 6 cut(s) 27, 76, 79, 82, 147, 614
MflI RGATCY 1 cut(s) 889
MluCI AATT 2 cut(s) 864, 897
Mph1103I ATGCAT 1 cut(s) 139
MseI TTAA 3 cut(s) 320, 387, 861
MspA1I CMGCKG 2 cut(s) 436, 1022
MspCI CTTAAG 1 cut(s) 386
MspI CCGG 3 cut(s) 395, 485, 797
MspR9I CCNGG 4 cut(s) 485, 749, 798, 999
MvaI CCWGG 2 cut(s) 749, 999
MwoI GCNNNNNNNGC 7 cut(s) 143, 242, 337, 704, 850, 1016, 1060
NciI CCSGG 2 cut(s) 485, 798
NdeII GATC 3 cut(s) 268, 568, 889
NlaIII CATG 1 cut(s) 404
NlaIV GGNNCC 3 cut(s) 557, 582, 658
NsiI ATGCAT 1 cut(s) 139
PaeR7I CTCGAG 1 cut(s) 876
PfeI GAWTC 2 cut(s) 391, 689
PfoI TCCNGGA 1 cut(s) 483
PkrI GCNGC 7 cut(s) 355, 432, 435, 451, 676, 1021, 1024
Psp6I CCWGG 2 cut(s) 747, 997
PspGI CCWGG 2 cut(s) 747, 997
PspN4I GGNNCC 3 cut(s) 557, 582, 658
PspPI GGNCC 2 cut(s) 533, 657
PspXI VCTCGAGB 1 cut(s) 876
PstI CTGCAG 1 cut(s) 959
PsuI RGATCY 1 cut(s) 889
PvuII CAGCTG 1 cut(s) 1022
RsaI GTAC 4 cut(s) 399, 440, 967, 981
RsaNI GTAC 4 cut(s) 398, 439, 966, 980
SaqAI TTAA 3 cut(s) 320, 387, 861
SatI GCNGC 7 cut(s) 354, 431, 434, 450, 675, 1020, 1023
Sau3AI GATC 3 cut(s) 268, 568, 889
Sau96I GGNCC 2 cut(s) 533, 657
ScrFI CCNGG 4 cut(s) 485, 749, 798, 999
SfaNI GCATC 2 cut(s) 146, 517
SfcI CTRYAG 2 cut(s) 51, 955
Sfr274I CTCGAG 1 cut(s) 876
SinI GGWCC 1 cut(s) 657
SlaI CTCGAG 1 cut(s) 876
SmlI CTYRAG 3 cut(s) 386, 876, 1039
SmoI CTYRAG 3 cut(s) 386, 876, 1039
Sse9I AATT 2 cut(s) 864, 897
SsiI CCGC 3 cut(s) 434, 660, 837
SspI AATATT 2 cut(s) 367, 948
SspMI CTAG 1 cut(s) 854
StyD4I CCNGG 4 cut(s) 483, 747, 796, 997
TaaI ACNGT 4 cut(s) 43, 409, 443, 514
TaiI ACGT 1 cut(s) 13
TaqI TCGA 3 cut(s) 162, 644, 877
TasI AATT 2 cut(s) 864, 897
TauI GCSGC 1 cut(s) 436
TfiI GAWTC 2 cut(s) 391, 689
Tru1I TTAA 3 cut(s) 320, 387, 861
Tru9I TTAA 3 cut(s) 320, 387, 861
TscAI CASTG 2 cut(s) 414, 543
TseI GCWGC 6 cut(s) 353, 430, 449, 674, 1019, 1022
TspDTI ATGAA 3 cut(s) 7, 36, 315
TspGWI ACGGA 2 cut(s) 97, 620
TspRI CASTG 2 cut(s) 414, 543
Vha464I CTTAAG 1 cut(s) 386
VpaK11BI GGWCC 1 cut(s) 657
XagI CCTNNNNNAGG 1 cut(s) 550
XapI RAATTY 1 cut(s) 897
XcmI CCANNNNNNNNNTGG 1 cut(s) 729
XhoI CTCGAG 1 cut(s) 876
XspI CTAG 1 cut(s) 854
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.