RLG00000022705

Methionyl-trna

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009304
Physical Location & Seq
Reverse (-)
10154211 .. 10160319
6109 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022705

Sequence Viewer

Length: 1089 bp
ATGTTGCGTCGGTTCTTGTGCTTCAATGCCTCGACTCCATCTTCTTCTTCTTCAATTCTATGCTTCTCTTCCAACAGGAATAGGAAGCCGCTCGTCTTCTTAGGCTCTCCTCAGGTCTCTGCAACTGTTCTTGACGCCCTGCTGAATGTATCCAGTGCCCCAGATTCTCTGTTTGAGGTTGCAGCAATTGTGACTCAACCACCTTCCAGGAAGAATAGGGGGAAGAAGGTGCTGCCATCTCCTTTAGCACAACATGCTCTTGACAGAGGCTTTCCTTCAGACCTCATTTTCACACCTGCACGAGCTGGAGAGGATATGTTCCTGTCCAGCTTAAGAGCTTTGCAGCCAGAACTTTGCATTACTGCCGCATACGGGAACATTTTACCTAGCAAGTTTCTTAATATCCCGACTTTGGGCACAGTAAATATACACCCAAGTTTGCTGCCGTTGTATCGTGGTGCAGCACCTGTTCAAAGAGCTTTGCAGGATGGTGTCAAAGAAACAGGAGTATCCTTAGCATTTACTGTCCGTGAGCTGGATGCTGGACCTGTGATAGCCAGTCAAAGAATGGAAATTGATGATCAAATTAAGGCACCAGATTTGCTTGCTCTCCTATTTTCTGAAGGATCCAAACTATTGATTCACCAGCTTCCTTCCGTATTTGATGGTTCAGCTAAATTAAAAGCGCAGTCCCAGGATGACTCTAAAGCTACATTAGCTCCAAAGATTTCTCCTGAGGAGTCATGGCTATCATTTGATCAGGAAGCTGTAGTCCTACACAACAAGGTTCGTGCATTTGCAGGATGGCCTGGAACTCGAGCTAAAGTTGTAGTTGTTGACAACAAAAACAGTCACCGGAATATGCTGGAGCTTAAAATTATCACAACCAAAGTTTGCAGCCATAGCAATTTTCAGGTCAACCAAGCAGATGACATCTCTTTTCGAAAGGATGCATTAGTATTCCCCTGTGGCCAGTCCACATCACTTGAGGTGCTGGAGATTCAGCTTCCTGGTAAGAAGGTTATGACTGCAGCTGCCTTTTGGAACGGTTTACGTGGTCAACAACTTAAAAGATTTGTTGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko00970 map00670 map00970
Pfam Domains
Protein Families
Formyl_trans_C Formyl_trans_N

Protein Analysis

363

Amino Acids

39.46

Weight (kDa)

9.17

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Formyl_trans_N PF00551 34 - 213 2.5e-32 Formyl transferase
Formyl_trans_C PF02911 242 - 351 4.4e-17 Formyl transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 304
Acc36I ACCTGC 1 cut(s) 304
AccB1I GGYRCC 1 cut(s) 592
AccBSI CCGCTC 1 cut(s) 91
AciI CCGC 2 cut(s) 89, 366
AclWI GGATC 2 cut(s) 621, 634
AcoI YGGCCR 1 cut(s) 970
AcuI CTGAAG 2 cut(s) 261, 642
AcyI GRCGYC 1 cut(s) 135
AfiI CCNNNNNNNGG 4 cut(s) 372, 412, 413, 535
AflII CTTAAG 1 cut(s) 331
AgsI TTSAA 3 cut(s) 25, 54, 473
AjnI CCWGG 4 cut(s) 206, 693, 808, 1009
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 2 cut(s) 621, 634
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 816
AoxI GGCC 2 cut(s) 806, 970
ApeKI GCWGC 8 cut(s) 182, 232, 343, 442, 461, 897, 1031, 1034
AspLEI GCGC 1 cut(s) 688
AspS9I GGNCC 1 cut(s) 545
AsuHPI GGTGA 2 cut(s) 635, 845
AsuII TTCGAA 1 cut(s) 943
AvaI CYCGRG 1 cut(s) 816
AvaII GGWCC 1 cut(s) 545
AxyI CCTNAGG 2 cut(s) 111, 735
BaeGI GKGCMC 2 cut(s) 160, 419
BaeI ACNNNNGTAYC 2 cut(s) 492, 525
BalI TGGCCA 1 cut(s) 972
BamHI GGATCC 1 cut(s) 626
BanI GGYRCC 1 cut(s) 592
BauI CACGAG 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 88
BbvI GCAGC 8 cut(s) 194, 219, 355, 429, 473, 909, 1021, 1043
BccI CCATC 5 cut(s) 46, 244, 482, 659, 798
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 4 cut(s) 208, 695, 810, 1011
BciVI GTATCC 2 cut(s) 160, 520
BclI TGATCA 2 cut(s) 580, 757
BcoDI GTCTC 1 cut(s) 121
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 2 cut(s) 768, 1029
BfrI CTTAAG 1 cut(s) 331
BfuAI ACCTGC 1 cut(s) 304
BfuI GTATCC 2 cut(s) 160, 520
Bme1390I CCNGG 4 cut(s) 208, 695, 810, 1011
Bme18I GGWCC 1 cut(s) 545
BmeT110I CYCGRG 1 cut(s) 816
BmgT120I GGNCC 1 cut(s) 545
BmiI GGNNCC 2 cut(s) 594, 628
BmrFI CCNGG 4 cut(s) 208, 695, 810, 1011
BmsI GCATC 2 cut(s) 529, 940
BpiI GAAGAC 1 cut(s) 88
BpmI CTGGAG 3 cut(s) 327, 887, 1016
Bpu10I CCTNAGC 1 cut(s) 514
Bpu14I TTCGAA 1 cut(s) 943
BpuEI CTTGAG 1 cut(s) 1007
BsaAI YACGTR 1 cut(s) 1055
BsaHI GRCGYC 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 693
BsaWI WCCGGW 1 cut(s) 855
BsaXI ACNNNNNCTCC 2 cut(s) 703, 733
Bsc4I CCNNNNNNNGG 4 cut(s) 372, 412, 413, 535
Bse1I ACTGG 3 cut(s) 153, 558, 973
Bse21I CCTNAGG 2 cut(s) 111, 735
BseBI CCWGG 4 cut(s) 208, 695, 810, 1011
BseDI CCNNGG 1 cut(s) 693
BseGI GGATG 5 cut(s) 493, 544, 703, 809, 955
BseLI CCNNNNNNNGG 4 cut(s) 372, 412, 413, 535
BseMII CTCAG 2 cut(s) 125, 726
BseNI ACTGG 3 cut(s) 153, 558, 973
BseRI GAGGAG 2 cut(s) 99, 752
BseSI GKGCMC 2 cut(s) 160, 419
BseXI GCAGC 8 cut(s) 194, 219, 355, 429, 473, 909, 1021, 1043
BsgI GTGCAG 2 cut(s) 282, 480
BshFI GGCC 2 cut(s) 808, 972
BshNI GGYRCC 1 cut(s) 592
BsiHKCI CYCGRG 1 cut(s) 816
BsiSI CCGG 1 cut(s) 856
BslFI GGGAC 1 cut(s) 676
BslI CCNNNNNNNGG 4 cut(s) 372, 412, 413, 535
BsmAI GTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 676
BsnI GGCC 2 cut(s) 808, 972
Bso31I GGTCTC 1 cut(s) 121
BsoBI CYCGRG 1 cut(s) 816
Bsp119I TTCGAA 1 cut(s) 943
Bsp1286I GDGCHC 2 cut(s) 160, 419
Bsp143I GATC 3 cut(s) 580, 626, 757
BspACI CCGC 2 cut(s) 89, 366
BspANI GGCC 2 cut(s) 808, 972
BspCNI CTCAG 2 cut(s) 124, 727
BspLI GGNNCC 2 cut(s) 594, 628
BspMAI CTGCAG 1 cut(s) 1033
BspMI ACCTGC 1 cut(s) 304
BspPI GGATC 2 cut(s) 621, 634
BspT104I TTCGAA 1 cut(s) 943
BspT107I GGYRCC 1 cut(s) 592
BspTI CTTAAG 1 cut(s) 331
BspTNI GGTCTC 1 cut(s) 121
BsrBI CCGCTC 1 cut(s) 91
BsrI ACTGG 3 cut(s) 153, 558, 973
BssECI CCNNGG 1 cut(s) 693
BssMI GATC 3 cut(s) 580, 626, 757
BssNI GRCGYC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 300
Bst2UI CCWGG 4 cut(s) 208, 695, 810, 1011
Bst4CI ACNGT 5 cut(s) 127, 421, 526, 851, 1049
Bst6I CTCTTC 1 cut(s) 73
BstACI GRCGYC 1 cut(s) 135
BstAFI CTTAAG 1 cut(s) 331
BstAPI GCANNNNNTGC 1 cut(s) 254
BstBAI YACGTR 1 cut(s) 1055
BstBI TTCGAA 1 cut(s) 943
BstC8I GCNNGC 1 cut(s) 606
BstDEI CTNAG 4 cut(s) 100, 111, 514, 735
BstF5I GGATG 5 cut(s) 493, 544, 703, 809, 955
BstHHI GCGC 1 cut(s) 688
BstKTI GATC 3 cut(s) 583, 629, 760
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 3 cut(s) 580, 626, 757
BstMWI GCNNNNNNNGC 3 cut(s) 254, 716, 903
BstNI CCWGG 4 cut(s) 208, 695, 810, 1011
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 4 cut(s) 206, 693, 808, 1009
BstSFI CTRYAG 2 cut(s) 768, 1029
BstSLI GKGCMC 2 cut(s) 160, 419
BstV1I GCAGC 8 cut(s) 194, 219, 355, 429, 473, 909, 1021, 1043
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 626
BstYI RGATCY 1 cut(s) 626
Bsu36I CCTNAGG 2 cut(s) 111, 735
BsuI GTATCC 2 cut(s) 160, 520
BsuRI GGCC 2 cut(s) 808, 972
BtsCI GGATG 5 cut(s) 493, 544, 703, 809, 955
BtsIMutI CAGTG 1 cut(s) 160
BveI ACCTGC 1 cut(s) 304
Cac8I GCNNGC 1 cut(s) 606
CaiI CAGNNNCTG 1 cut(s) 467
CfoI GCGC 1 cut(s) 688
Cfr13I GGNCC 1 cut(s) 545
CseI GACGC 1 cut(s) 143
CspCI CAANNNNNGTGG 2 cut(s) 967, 1002
CviAII CATG 2 cut(s) 254, 744
DdeI CTNAG 4 cut(s) 100, 111, 514, 735
DpnI GATC 3 cut(s) 582, 628, 759
DpnII GATC 3 cut(s) 580, 626, 757
EaeI YGGCCR 1 cut(s) 970
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 545
Eco57I CTGAAG 2 cut(s) 261, 642
Eco81I CCTNAGG 2 cut(s) 111, 735
Eco88I CYCGRG 1 cut(s) 816
EcoRII CCWGG 4 cut(s) 206, 693, 808, 1009
EcoT22I ATGCAT 1 cut(s) 955
FaeI CATG 2 cut(s) 257, 747
FaiI YATR 9 cut(s) 61, 255, 317, 370, 428, 745, 863, 903, 1025
FaqI GGGAC 1 cut(s) 676
FatI CATG 2 cut(s) 253, 743
FbaI TGATCA 2 cut(s) 580, 757
FokI GGATG 5 cut(s) 500, 551, 710, 816, 962
FspBI CTAG 1 cut(s) 387
GlaI GCGC 1 cut(s) 687
GsuI CTGGAG 3 cut(s) 327, 887, 1016
HaeIII GGCC 2 cut(s) 808, 972
HapII CCGG 1 cut(s) 856
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 688
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 2 cut(s) 257, 747
Hin6I GCGC 1 cut(s) 686
HinP1I GCGC 1 cut(s) 686
HincII GTYRAC 3 cut(s) 838, 919, 1061
HindII GTYRAC 3 cut(s) 838, 919, 1061
HinfI GANTC 7 cut(s) 34, 164, 193, 640, 701, 740, 1000
HpaII CCGG 1 cut(s) 856
HphI GGTGA 2 cut(s) 635, 845
Hpy166II GTNNAC 5 cut(s) 838, 919, 978, 1052, 1061
Hpy188I TCNGA 2 cut(s) 280, 622
Hpy188III TCNNGA 5 cut(s) 131, 260, 406, 734, 761
Hpy8I GTNNAC 5 cut(s) 838, 919, 978, 1052, 1061
Hpy99I CGWCG 1 cut(s) 12
HpyAV CCTTC 6 cut(s) 213, 220, 285, 617, 663, 1012
HpyCH4III ACNGT 5 cut(s) 127, 421, 526, 851, 1049
HpyCH4IV ACGT 1 cut(s) 1054
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 716, 903
HpyF3I CTNAG 4 cut(s) 100, 111, 514, 735
HpySE526I ACGT 1 cut(s) 1054
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 2 cut(s) 257, 747
HspAI GCGC 1 cut(s) 686
Ksp22I TGATCA 2 cut(s) 580, 757
Kzo9I GATC 3 cut(s) 580, 626, 757
LmnI GCTCC 2 cut(s) 724, 868
Lsp1109I GCAGC 8 cut(s) 194, 219, 355, 429, 473, 909, 1021, 1043
LweI GCATC 2 cut(s) 529, 940
MaeI CTAG 1 cut(s) 387
MaeII ACGT 1 cut(s) 1054
MaeIII GTNAC 2 cut(s) 190, 851
MalI GATC 3 cut(s) 582, 628, 759
MbiI CCGCTC 1 cut(s) 91
MboI GATC 3 cut(s) 580, 626, 757
MboII GAAGA 8 cut(s) 33, 36, 39, 42, 60, 88, 223, 235
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 1 cut(s) 626
MhlI GDGCHC 2 cut(s) 160, 419
MlsI TGGCCA 1 cut(s) 972
MluCI AATT 7 cut(s) 54, 186, 573, 585, 677, 876, 907
MluNI TGGCCA 1 cut(s) 972
MlyI GAGTC 4 cut(s) 28, 187, 695, 749
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 8 cut(s) 40, 120, 169, 260, 293, 304, 730, 982
Mox20I TGGCCA 1 cut(s) 972
Mph1103I ATGCAT 1 cut(s) 955
MscI TGGCCA 1 cut(s) 972
MseI TTAA 6 cut(s) 332, 399, 588, 680, 873, 1068
Msp20I TGGCCA 1 cut(s) 972
MspA1I CMGCKG 1 cut(s) 1034
MspCI CTTAAG 1 cut(s) 331
MspI CCGG 1 cut(s) 856
MspR9I CCNGG 4 cut(s) 208, 695, 810, 1011
MunI CAATTG 1 cut(s) 186
MvaI CCWGG 4 cut(s) 208, 695, 810, 1011
MwoI GCNNNNNNNGC 3 cut(s) 254, 716, 903
NdeII GATC 3 cut(s) 580, 626, 757
NlaIII CATG 2 cut(s) 257, 747
NlaIV GGNNCC 2 cut(s) 594, 628
NmuCI GTSAC 2 cut(s) 190, 851
NsiI ATGCAT 1 cut(s) 955
NspI RCATGY 1 cut(s) 257
NspV TTCGAA 1 cut(s) 943
PaeR7I CTCGAG 1 cut(s) 816
PaqCI CACCTGC 1 cut(s) 304
PfeI GAWTC 3 cut(s) 164, 640, 1000
PfoI TCCNGGA 1 cut(s) 206
PleI GAGTC 4 cut(s) 28, 187, 695, 748
PpsI GAGTC 4 cut(s) 28, 187, 695, 748
Ppu21I YACGTR 1 cut(s) 1055
Psp6I CCWGG 4 cut(s) 206, 693, 808, 1009
PspGI CCWGG 4 cut(s) 206, 693, 808, 1009
PspN4I GGNNCC 2 cut(s) 594, 628
PspPI GGNCC 1 cut(s) 545
PspXI VCTCGAGB 1 cut(s) 816
PstI CTGCAG 1 cut(s) 1033
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 1 cut(s) 626
PvuII CAGCTG 1 cut(s) 1034
SaqAI TTAA 6 cut(s) 332, 399, 588, 680, 873, 1068
Sau3AI GATC 3 cut(s) 580, 626, 757
Sau96I GGNCC 1 cut(s) 545
SchI GAGTC 4 cut(s) 28, 187, 695, 749
ScrFI CCNGG 4 cut(s) 208, 695, 810, 1011
SduI GDGCHC 2 cut(s) 160, 419
SfaNI GCATC 2 cut(s) 529, 940
SfcI CTRYAG 2 cut(s) 768, 1029
Sfr274I CTCGAG 1 cut(s) 816
SfuI TTCGAA 1 cut(s) 943
SinI GGWCC 1 cut(s) 545
SlaI CTCGAG 1 cut(s) 816
SmlI CTYRAG 3 cut(s) 331, 816, 986
SmoI CTYRAG 3 cut(s) 331, 816, 986
Sse9I AATT 7 cut(s) 54, 186, 573, 585, 677, 876, 907
SsiI CCGC 2 cut(s) 89, 366
SspMI CTAG 1 cut(s) 387
StyD4I CCNGG 4 cut(s) 206, 693, 808, 1009
TaaI ACNGT 5 cut(s) 127, 421, 526, 851, 1049
TaiI ACGT 1 cut(s) 1057
TaqI TCGA 3 cut(s) 32, 817, 943
TasI AATT 7 cut(s) 54, 186, 573, 585, 677, 876, 907
TauI GCSGC 2 cut(s) 91, 368
TfiI GAWTC 3 cut(s) 164, 640, 1000
Tru1I TTAA 6 cut(s) 332, 399, 588, 680, 873, 1068
Tru9I TTAA 6 cut(s) 332, 399, 588, 680, 873, 1068
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 2 cut(s) 190, 851
TseI GCWGC 8 cut(s) 182, 232, 343, 442, 461, 897, 1031, 1034
Tsp45I GTSAC 2 cut(s) 190, 851
TspGWI ACGGA 2 cut(s) 518, 646
TspRI CASTG 1 cut(s) 160
Vha464I CTTAAG 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 545
XceI RCATGY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 565
XhoI CTCGAG 1 cut(s) 816
XspI CTAG 1 cut(s) 387
Zsp2I ATGCAT 1 cut(s) 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.