pycom09g17510

Methionyl-trna

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0009304
Physical Location & Seq
Reverse (-)
18012391 .. 18017329
4939 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g17510.9

Sequence Viewer

Length: 1125 bp
ATGAATTCGTCGCTGGTGTTTCGTCGCTTCTGGTGCTTCAGTGCCGCAACTTCTTCTTCATCTTCTTCTTCTTCTACTTTATGCTTCGCTTCCAACAAGAAGCCCCTCGTCTTCTTAGGCTCTCCTCAGGTGTCAGCAACTGTTCTGGATGCCCTTCTCAATGCATCCACAACCGCAGACTCACTCTTTGAGGTTGCAGCTATTGTTACTCAGCCCCCATCCAGAAAGTCTAGGGGGAAAAAGTTGATGCCTTCTCCTTTAGCACAGCATGCTCTTGACAGAGGCTTCCCTTCAAACCTTATTTTCACACCTGAACGGGCTGGAGAGGATGTGTTTTTGTCCGACTTAAGAGCTTTGCAGCCACAACTTTGCATCACTGCTGCTTATGGGAACATTTTACCTACCAAGTTTCTCAATATCCCAACATTGGGTACGGTCAATATACACCCAAGTTTGCTTCCATTGTATCGTGGAGCTGCACCTGTTCAAAGAGCCTTGCAGGATGGTGTTAATGAAACAGGAGTATCCTTAGCGTTCACTGTTCGTGCACTGGATGCCGGGCCTGTCATTGCCTGTGAAAGAATGGAAATTGATGATCAAATTAAGGCACCAGATCTACTTGCACTACTATTTAATGAAGGATCTAAACTATTGATCAACGAGCTTCCTTCTATATTTGATGGGTCAGCTAAGGTGAAAGCATGTCCCCAGGATGACTCTAAAGCTACCTTAGCTCCCAAGATTGCTCCGGAAGAATCGTGGCTGTCCTTTGATCAGGAGGCTTCAGTTCTACATAATAAGGTTCGTGCATTTGCAGGGTGGCCTGGAACTCGAGCTAAAGTTGTAATTATTGACAACAAAACTGGTCAAGAGAATATCATGGAATTCAAAATTATGACAACTAAAGTCTGCAGCCAGAGCAATGTTCAGGTCAACCAAGCGGATGAAATCACCTTTATAAAGCCTGCATTAGTATTTCCCTGTGGACGGGGCACAGCCCTTGAGGTATTGGAGGTTCAGATTCCGGGTAAGAAGGCAATAAGTGCAGCTGCCTTCTGGAATGGTTTGCGTGGTCAAAAACTGAAGATATACCATGCTTCCAATGTAAACGGTAATTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko00970 map00670 map00970
Pfam Domains
Protein Families
Formyl_trans_C Formyl_trans_N

Protein Analysis

375

Amino Acids

40.24

Weight (kDa)

8.77

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 959
AccB1I GGYRCC 1 cut(s) 607
AccIII TCCGGA 1 cut(s) 748
AciI CCGC 3 cut(s) 45, 174, 941
AclWI GGATC 1 cut(s) 649
AcsI RAATTY 2 cut(s) 4, 884
AcuI CTGAAG 3 cut(s) 22, 768, 1103
AfaI GTAC 1 cut(s) 433
AfiI CCNNNNNNNGG 3 cut(s) 427, 428, 987
AflII CTTAAG 1 cut(s) 346
AgsI TTSAA 3 cut(s) 294, 488, 889
AjnI CCWGG 2 cut(s) 708, 823
AluBI AGCT 9 cut(s) 200, 353, 476, 664, 689, 725, 734, 836, 1049
AluI AGCT 9 cut(s) 200, 353, 476, 664, 689, 725, 734, 836, 1049
Alw21I GWGCWC 1 cut(s) 550
Alw44I GTGCAC 1 cut(s) 546
AlwI GGATC 1 cut(s) 649
AlwNI CAGNNNCTG 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 831
Aor13HI TCCGGA 1 cut(s) 748
AoxI GGCC 2 cut(s) 560, 821
ApaLI GTGCAC 1 cut(s) 546
ApeKI GCWGC 7 cut(s) 197, 358, 380, 476, 912, 1046, 1049
ApoI RAATTY 2 cut(s) 4, 884
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AspS9I GGNCC 1 cut(s) 560
AsuC2I CCSGG 2 cut(s) 559, 1026
AsuHPI GGTGA 2 cut(s) 706, 943
AvaI CYCGRG 1 cut(s) 831
AxyI CCTNAGG 1 cut(s) 126
BaeGI GKGCMC 2 cut(s) 550, 995
BaeI ACNNNNGTAYC 2 cut(s) 507, 540
BanI GGYRCC 1 cut(s) 607
BbsI GAAGAC 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 7 cut(s) 209, 367, 370, 463, 924, 1036, 1058
BccI CCATC 3 cut(s) 226, 497, 674
BciT130I CCWGG 2 cut(s) 710, 825
BciVI GTATCC 1 cut(s) 535
BclI TGATCA 3 cut(s) 595, 654, 772
BcnI CCSGG 2 cut(s) 559, 1026
BfaI CTAG 2 cut(s) 231, 1119
BfmI CTRYAG 1 cut(s) 910
BfrI CTTAAG 1 cut(s) 346
BfuI GTATCC 1 cut(s) 535
BglII AGATCT 1 cut(s) 613
BisI GCNGC 8 cut(s) 45, 198, 359, 381, 477, 913, 1047, 1050
BlsI GCNGC 8 cut(s) 46, 199, 360, 382, 478, 914, 1048, 1051
Bme1390I CCNGG 4 cut(s) 559, 710, 825, 1026
BmeT110I CYCGRG 1 cut(s) 831
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 1 cut(s) 609
BmrFI CCNGG 4 cut(s) 559, 710, 825, 1026
BmsI GCATC 5 cut(s) 139, 173, 237, 381, 544
BpiI GAAGAC 1 cut(s) 103
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 3 cut(s) 529, 690, 730
BpuEI CTTGAG 1 cut(s) 1022
BpuMI CCSGG 2 cut(s) 559, 1026
BsaJI CCNNGG 1 cut(s) 708
BsaWI WCCGGW 1 cut(s) 748
BsaXI ACNNNNNCTCC 4 cut(s) 315, 345, 718, 748
Bsc4I CCNNNNNNNGG 3 cut(s) 427, 428, 987
Bse1I ACTGG 2 cut(s) 555, 868
Bse21I CCTNAGG 1 cut(s) 126
Bse3DI GCAATG 2 cut(s) 567, 928
BseAI TCCGGA 1 cut(s) 748
BseBI CCWGG 2 cut(s) 710, 825
BseDI CCNNGG 1 cut(s) 708
BseGI GGATG 8 cut(s) 154, 164, 218, 334, 508, 559, 718, 949
BseLI CCNNNNNNNGG 3 cut(s) 427, 428, 987
BseMI GCAATG 2 cut(s) 567, 928
BseMII CTCAG 2 cut(s) 140, 224
BseNI ACTGG 2 cut(s) 555, 868
BseRI GAGGAG 1 cut(s) 114
BseSI GKGCMC 2 cut(s) 550, 995
BseXI GCAGC 7 cut(s) 209, 367, 370, 463, 924, 1036, 1058
BsgI GTGCAG 2 cut(s) 462, 1065
BshFI GGCC 2 cut(s) 562, 823
BshNI GGYRCC 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 550
BsiHKCI CYCGRG 1 cut(s) 831
BsiSI CCGG 3 cut(s) 558, 749, 1025
BslFI GGGAC 1 cut(s) 690
BslI CCNNNNNNNGG 3 cut(s) 427, 428, 987
BsmFI GGGAC 1 cut(s) 690
BsnI GGCC 2 cut(s) 562, 823
BsoBI CYCGRG 1 cut(s) 831
Bsp1286I GDGCHC 2 cut(s) 550, 995
Bsp13I TCCGGA 1 cut(s) 748
Bsp143I GATC 5 cut(s) 595, 613, 641, 654, 772
BspACI CCGC 3 cut(s) 45, 174, 941
BspANI GGCC 2 cut(s) 562, 823
BspCNI CTCAG 2 cut(s) 139, 223
BspEI TCCGGA 1 cut(s) 748
BspLI GGNNCC 1 cut(s) 609
BspMAI CTGCAG 1 cut(s) 914
BspPI GGATC 1 cut(s) 649
BspT107I GGYRCC 1 cut(s) 607
BspTI CTTAAG 1 cut(s) 346
BsrDI GCAATG 2 cut(s) 567, 928
BsrI ACTGG 2 cut(s) 555, 868
BssECI CCNNGG 1 cut(s) 708
BssMI GATC 5 cut(s) 595, 613, 641, 654, 772
Bst2UI CCWGG 2 cut(s) 710, 825
Bst4CI ACNGT 4 cut(s) 142, 436, 541, 1112
BstAFI CTTAAG 1 cut(s) 346
BstAPI GCANNNNNTGC 3 cut(s) 269, 554, 1043
BstC8I GCNNGC 2 cut(s) 270, 966
BstDEI CTNAG 6 cut(s) 115, 126, 210, 529, 690, 730
BstF5I GGATG 8 cut(s) 154, 164, 218, 334, 508, 559, 718, 949
BstKTI GATC 5 cut(s) 598, 616, 644, 657, 775
BstMBI GATC 5 cut(s) 595, 613, 641, 654, 772
BstMWI GCNNNNNNNGC 6 cut(s) 33, 269, 554, 731, 918, 1043
BstNI CCWGG 2 cut(s) 710, 825
BstNSI RCATGY 2 cut(s) 272, 705
BstSCI CCNGG 4 cut(s) 557, 708, 823, 1024
BstSFI CTRYAG 1 cut(s) 910
BstSLI GKGCMC 2 cut(s) 550, 995
BstV1I GCAGC 7 cut(s) 209, 367, 370, 463, 924, 1036, 1058
BstV2I GAAGAC 1 cut(s) 103
BstX2I RGATCY 2 cut(s) 613, 641
BstYI RGATCY 2 cut(s) 613, 641
Bsu36I CCTNAGG 1 cut(s) 126
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 2 cut(s) 562, 823
BtsCI GGATG 8 cut(s) 154, 164, 218, 334, 508, 559, 718, 949
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 4 cut(s) 46, 375, 537, 548
Cac8I GCNNGC 2 cut(s) 270, 966
CaiI CAGNNNCTG 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 560
Csp6I GTAC 1 cut(s) 432
CviAII CATG 4 cut(s) 269, 702, 880, 1094
CviQI GTAC 1 cut(s) 432
DdeI CTNAG 6 cut(s) 115, 126, 210, 529, 690, 730
DpnI GATC 5 cut(s) 597, 615, 643, 656, 774
DpnII GATC 5 cut(s) 595, 613, 641, 654, 772
Eco57I CTGAAG 3 cut(s) 22, 768, 1103
Eco81I CCTNAGG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 831
EcoRI GAATTC 2 cut(s) 4, 884
EcoRII CCWGG 2 cut(s) 708, 823
EcoT22I ATGCAT 1 cut(s) 166
FaeI CATG 4 cut(s) 272, 705, 883, 1097
FaqI GGGAC 1 cut(s) 690
FatI CATG 4 cut(s) 268, 701, 879, 1093
FbaI TGATCA 3 cut(s) 595, 654, 772
Fnu4HI GCNGC 8 cut(s) 45, 198, 359, 381, 477, 913, 1047, 1050
FokI GGATG 8 cut(s) 151, 161, 205, 341, 515, 566, 725, 956
Fsp4HI GCNGC 8 cut(s) 45, 198, 359, 381, 477, 913, 1047, 1050
FspBI CTAG 2 cut(s) 231, 1119
GluI GCNGC 8 cut(s) 45, 198, 359, 381, 477, 913, 1047, 1050
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 2 cut(s) 562, 823
HapII CCGG 3 cut(s) 558, 749, 1025
Hin1II CATG 4 cut(s) 272, 705, 883, 1097
HincII GTYRAC 1 cut(s) 934
HindII GTYRAC 1 cut(s) 934
HinfI GANTC 4 cut(s) 179, 716, 755, 1021
HpaII CCGG 3 cut(s) 558, 749, 1025
HphI GGTGA 2 cut(s) 706, 943
Hpy166II GTNNAC 5 cut(s) 537, 548, 934, 986, 1108
Hpy188I TCNGA 2 cut(s) 343, 1020
Hpy188III TCNNGA 7 cut(s) 146, 222, 275, 749, 776, 869, 1057
Hpy8I GTNNAC 5 cut(s) 537, 548, 934, 986, 1108
Hpy99I CGWCG 2 cut(s) 13, 27
HpyAV CCTTC 7 cut(s) 164, 261, 300, 632, 678, 1027, 1063
HpyCH4III ACNGT 4 cut(s) 142, 436, 541, 1112
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 269, 554, 731, 918, 1043
HpyF3I CTNAG 6 cut(s) 115, 126, 210, 529, 690, 730
Hsp92II CATG 4 cut(s) 272, 705, 883, 1097
Kpn2I TCCGGA 1 cut(s) 748
Ksp22I TGATCA 3 cut(s) 595, 654, 772
Kzo9I GATC 5 cut(s) 595, 613, 641, 654, 772
LmnI GCTCC 3 cut(s) 473, 739, 751
Lsp1109I GCAGC 7 cut(s) 209, 367, 370, 463, 924, 1036, 1058
LweI GCATC 5 cut(s) 139, 173, 237, 381, 544
MaeI CTAG 2 cut(s) 231, 1119
MaeIII GTNAC 1 cut(s) 205
MalI GATC 5 cut(s) 597, 615, 643, 656, 774
MboI GATC 5 cut(s) 595, 613, 641, 654, 772
MboII GAAGA 9 cut(s) 45, 48, 54, 57, 60, 63, 103, 764, 1096
MflI RGATCY 2 cut(s) 613, 641
MhlI GDGCHC 2 cut(s) 550, 995
MluCI AATT 7 cut(s) 4, 588, 600, 846, 884, 891, 1114
MlyI GAGTC 2 cut(s) 173, 710
MmeI TCCRAC 2 cut(s) 117, 366
MnlI CCTC 8 cut(s) 116, 135, 184, 275, 319, 772, 997, 1006
Mph1103I ATGCAT 1 cut(s) 166
MroI TCCGGA 1 cut(s) 748
MseI TTAA 4 cut(s) 347, 510, 603, 633
MspA1I CMGCKG 1 cut(s) 1049
MspCI CTTAAG 1 cut(s) 346
MspI CCGG 3 cut(s) 558, 749, 1025
MspR9I CCNGG 4 cut(s) 559, 710, 825, 1026
MvaI CCWGG 2 cut(s) 710, 825
MwoI GCNNNNNNNGC 6 cut(s) 33, 269, 554, 731, 918, 1043
NciI CCSGG 2 cut(s) 559, 1026
NdeII GATC 5 cut(s) 595, 613, 641, 654, 772
NlaIII CATG 4 cut(s) 272, 705, 883, 1097
NlaIV GGNNCC 1 cut(s) 609
NsiI ATGCAT 1 cut(s) 166
NspI RCATGY 2 cut(s) 272, 705
PaeI GCATGC 1 cut(s) 272
PaeR7I CTCGAG 1 cut(s) 831
PfeI GAWTC 2 cut(s) 755, 1021
PkrI GCNGC 8 cut(s) 46, 199, 360, 382, 478, 914, 1048, 1051
PleI GAGTC 2 cut(s) 173, 710
PpsI GAGTC 2 cut(s) 173, 710
PsiI TTATAA 1 cut(s) 959
Psp6I CCWGG 2 cut(s) 708, 823
PspGI CCWGG 2 cut(s) 708, 823
PspN4I GGNNCC 1 cut(s) 609
PspPI GGNCC 1 cut(s) 560
PspXI VCTCGAGB 1 cut(s) 831
PsrI GAACNNNNNNTAC 2 cut(s) 999, 1031
PstI CTGCAG 1 cut(s) 914
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 2 cut(s) 613, 641
PvuII CAGCTG 1 cut(s) 1049
RsaI GTAC 1 cut(s) 433
RsaNI GTAC 1 cut(s) 432
SaqAI TTAA 4 cut(s) 347, 510, 603, 633
SatI GCNGC 8 cut(s) 45, 198, 359, 381, 477, 913, 1047, 1050
Sau3AI GATC 5 cut(s) 595, 613, 641, 654, 772
Sau96I GGNCC 1 cut(s) 560
SchI GAGTC 2 cut(s) 173, 710
ScrFI CCNGG 4 cut(s) 559, 710, 825, 1026
SduI GDGCHC 2 cut(s) 550, 995
SfaNI GCATC 5 cut(s) 139, 173, 237, 381, 544
SfcI CTRYAG 1 cut(s) 910
Sfr274I CTCGAG 1 cut(s) 831
SlaI CTCGAG 1 cut(s) 831
SmlI CTYRAG 3 cut(s) 346, 831, 1001
SmoI CTYRAG 3 cut(s) 346, 831, 1001
SphI GCATGC 1 cut(s) 272
Sse9I AATT 7 cut(s) 4, 588, 600, 846, 884, 891, 1114
SsiI CCGC 3 cut(s) 45, 174, 941
SspMI CTAG 2 cut(s) 231, 1119
StyD4I CCNGG 4 cut(s) 557, 708, 823, 1024
TaaI ACNGT 4 cut(s) 142, 436, 541, 1112
TaqI TCGA 1 cut(s) 832
TasI AATT 7 cut(s) 4, 588, 600, 846, 884, 891, 1114
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 2 cut(s) 755, 1021
Tru1I TTAA 4 cut(s) 347, 510, 603, 633
Tru9I TTAA 4 cut(s) 347, 510, 603, 633
TscAI CASTG 4 cut(s) 46, 382, 544, 555
TseI GCWGC 7 cut(s) 197, 358, 380, 476, 912, 1046, 1049
TspDTI ATGAA 5 cut(s) 17, 48, 528, 651, 960
TspRI CASTG 4 cut(s) 46, 382, 544, 555
Vha464I CTTAAG 1 cut(s) 346
VneI GTGCAC 1 cut(s) 546
XapI RAATTY 2 cut(s) 4, 884
XceI RCATGY 2 cut(s) 272, 705
XhoI CTCGAG 1 cut(s) 831
XspI CTAG 2 cut(s) 231, 1119
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.