Rh3BG347100

Methionyl-trna

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0009304
Physical Location & Seq
Forward (+)
38098597 .. 38105507
6911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG347100.1

Sequence Viewer

Length: 906 bp
ATGAATTCCTCCTCACTGATGTTGCGTCGGTTCTTGTGCTTCAATGCCTTGACTGCATCTTCTTCTTCTATTCTATGCTTCTCCTCCAACAGGAATAGGAAGCCCCTCGTCTTCTTAGGCACTCCTCAGGTCTCTACAACTGTTCTTGATGCCCTGATGAATGCATCCGGTGCCCCAGATTCTCTGTTTGAGGTTGCAGCAATTGTGACTCAACCACCTTCCAGGAAGAATAGGGGGAAGAAGGTGCTGTCGTCTCCTTTAGCACAACGTGCTCTTGACAGAGGCTTTCCTTCAGACCTCATTTTCACACCTGCACGAGCTGGAGAGGATATGTTCCTGTCCAGCTTAAGAGCTTTGCAGCCAGAACTTTGTATCACTGCCGCATATGGGAGCATTTTACCTAGCAAGTTTCTTAATATCCCGACTTTGGGCACAGTAAATATACACCCAAGTTTGCTGCCGTTGTATCGTGGTGCAGCACCTGTTCAAAGAGCTTTGCAGGATGGTGTCAAAGAAACAGGAGTATCCTTAGCATTTACTGTCCGTACGCTGGATGCTGGACCTGTGATAGCCAGTCAAAGACTGGAAATTGATGATCAAATTAAGGCACCAGATTTGCTTACTCTCCTATTTTCTGAAGGATCCAAACTATTGATTCACCAGCTTCCTTCCATATTTGATGGTTCAGCTAAATTAAAAGCGCAGTCCCAGGATGACTCTAAAGCTACATTAGCTCCAAAGATTTCTCCTGAGGAGTCATGGCTATCATTTGATCAGGAAGCTGTAGTCCTACATAACAAGGTATGGTCTTTCCTTTTCCTAAGATTTTATTACAGAGTGATAACTTGCAACCTCTATAGGTTCGTGCATTTGCAGGATGGCCTGGAACTCGAGCTAAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko00970 map00670 map00970
Pfam Domains
Protein Families
Formyl_trans_C Formyl_trans_N

Protein Analysis

301

Amino Acids

33.07

Weight (kDa)

9.04

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Formyl_trans_N PF00551 37 - 210 1.3e-31 Formyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 319
Acc36I ACCTGC 1 cut(s) 319
AccB1I GGYRCC 2 cut(s) 170, 607
AciI CCGC 1 cut(s) 381
AclWI GGATC 2 cut(s) 636, 649
AcsI RAATTY 1 cut(s) 4
AcuI CTGAAG 2 cut(s) 276, 657
AdeI CACNNNGTG 1 cut(s) 269
AfaI GTAC 1 cut(s) 547
AfiI CCNNNNNNNGG 5 cut(s) 90, 387, 427, 428, 550
AflII CTTAAG 1 cut(s) 346
AgsI TTSAA 2 cut(s) 43, 488
AjnI CCWGG 3 cut(s) 221, 708, 882
Alw21I GWGCWC 1 cut(s) 274
Alw26I GTCTC 2 cut(s) 136, 258
AlwI GGATC 2 cut(s) 636, 649
AlwNI CAGNNNCTG 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 890
AoxI GGCC 1 cut(s) 880
ApeKI GCWGC 4 cut(s) 197, 358, 457, 476
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 1 cut(s) 703
AspS9I GGNCC 1 cut(s) 560
AsuHPI GGTGA 1 cut(s) 650
AvaI CYCGRG 1 cut(s) 890
AvaII GGWCC 1 cut(s) 560
AxyI CCTNAGG 2 cut(s) 126, 750
BaeGI GKGCMC 2 cut(s) 175, 434
BaeI ACNNNNGTAYC 2 cut(s) 507, 540
BamHI GGATCC 1 cut(s) 641
BanI GGYRCC 2 cut(s) 170, 607
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 274
BbvI GCAGC 4 cut(s) 209, 370, 444, 488
BccI CCATC 3 cut(s) 497, 674, 872
BceAI ACGGC 1 cut(s) 445
BciT130I CCWGG 3 cut(s) 223, 710, 884
BciVI GTATCC 1 cut(s) 535
BclI TGATCA 2 cut(s) 595, 772
BcoDI GTCTC 2 cut(s) 136, 258
BfaI CTAG 1 cut(s) 402
BfmI CTRYAG 2 cut(s) 783, 856
BfrI CTTAAG 1 cut(s) 346
BfuAI ACCTGC 1 cut(s) 319
BfuI GTATCC 1 cut(s) 535
BisI GCNGC 5 cut(s) 198, 359, 381, 458, 477
BlsI GCNGC 5 cut(s) 199, 360, 382, 459, 478
Bme1390I CCNGG 3 cut(s) 223, 710, 884
Bme18I GGWCC 1 cut(s) 560
BmeT110I CYCGRG 1 cut(s) 890
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 3 cut(s) 172, 609, 643
BmrFI CCNGG 3 cut(s) 223, 710, 884
BmsI GCATC 4 cut(s) 65, 139, 173, 544
BpiI GAAGAC 1 cut(s) 103
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 529
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 708
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 2 cut(s) 718, 748
Bsc4I CCNNNNNNNGG 5 cut(s) 90, 387, 427, 428, 550
Bse1I ACTGG 2 cut(s) 573, 588
Bse21I CCTNAGG 2 cut(s) 126, 750
BseBI CCWGG 3 cut(s) 223, 710, 884
BseDI CCNNGG 1 cut(s) 708
BseGI GGATG 5 cut(s) 164, 508, 559, 718, 883
BseLI CCNNNNNNNGG 5 cut(s) 90, 387, 427, 428, 550
BseMII CTCAG 2 cut(s) 140, 741
BseNI ACTGG 2 cut(s) 573, 588
BseRI GAGGAG 3 cut(s) 73, 114, 767
BseSI GKGCMC 2 cut(s) 175, 434
BseXI GCAGC 4 cut(s) 209, 370, 444, 488
BsgI GTGCAG 2 cut(s) 297, 495
BshFI GGCC 1 cut(s) 882
BshNI GGYRCC 2 cut(s) 170, 607
BsiHKAI GWGCWC 1 cut(s) 274
BsiHKCI CYCGRG 1 cut(s) 890
BsiSI CCGG 1 cut(s) 168
BsiWI CGTACG 1 cut(s) 545
BslFI GGGAC 1 cut(s) 691
BslI CCNNNNNNNGG 5 cut(s) 90, 387, 427, 428, 550
BsmAI GTCTC 2 cut(s) 136, 258
BsmBI CGTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 691
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 1 cut(s) 882
Bso31I GGTCTC 1 cut(s) 136
BsoBI CYCGRG 1 cut(s) 890
Bsp1286I GDGCHC 3 cut(s) 175, 274, 434
Bsp143I GATC 3 cut(s) 595, 641, 772
BspACI CCGC 1 cut(s) 381
BspANI GGCC 1 cut(s) 882
BspCNI CTCAG 2 cut(s) 139, 742
BspLI GGNNCC 3 cut(s) 172, 609, 643
BspMI ACCTGC 1 cut(s) 319
BspPI GGATC 2 cut(s) 636, 649
BspT107I GGYRCC 2 cut(s) 170, 607
BspTI CTTAAG 1 cut(s) 346
BspTNI GGTCTC 1 cut(s) 136
BsrI ACTGG 2 cut(s) 573, 588
BssECI CCNNGG 1 cut(s) 708
BssMI GATC 3 cut(s) 595, 641, 772
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 3 cut(s) 223, 710, 884
Bst4CI ACNGT 3 cut(s) 142, 436, 541
BstAFI CTTAAG 1 cut(s) 346
BstAPI GCANNNNNTGC 2 cut(s) 170, 269
BstDEI CTNAG 5 cut(s) 115, 126, 529, 750, 821
BstENI CCTNNNNNAGG 1 cut(s) 88
BstF5I GGATG 5 cut(s) 164, 508, 559, 718, 883
BstHHI GCGC 1 cut(s) 703
BstKTI GATC 3 cut(s) 598, 644, 775
BstMAI GTCTC 2 cut(s) 136, 258
BstMBI GATC 3 cut(s) 595, 641, 772
BstMWI GCNNNNNNNGC 4 cut(s) 53, 170, 269, 731
BstNI CCWGG 3 cut(s) 223, 710, 884
BstSCI CCNGG 3 cut(s) 221, 708, 882
BstSFI CTRYAG 2 cut(s) 783, 856
BstSLI GKGCMC 2 cut(s) 175, 434
BstV1I GCAGC 4 cut(s) 209, 370, 444, 488
BstV2I GAAGAC 1 cut(s) 103
BstX2I RGATCY 1 cut(s) 641
BstYI RGATCY 1 cut(s) 641
Bsu36I CCTNAGG 2 cut(s) 126, 750
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 1 cut(s) 882
BtsCI GGATG 5 cut(s) 164, 508, 559, 718, 883
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 14, 375
BveI ACCTGC 1 cut(s) 319
CaiI CAGNNNCTG 1 cut(s) 482
CfoI GCGC 1 cut(s) 703
Cfr13I GGNCC 1 cut(s) 560
CseI GACGC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 546
CviAII CATG 1 cut(s) 759
CviQI GTAC 1 cut(s) 546
DdeI CTNAG 5 cut(s) 115, 126, 529, 750, 821
DpnI GATC 3 cut(s) 597, 643, 774
DpnII GATC 3 cut(s) 595, 641, 772
DraIII CACNNNGTG 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 560
Eco57I CTGAAG 2 cut(s) 276, 657
Eco81I CCTNAGG 2 cut(s) 126, 750
Eco88I CYCGRG 1 cut(s) 890
EcoNI CCTNNNNNAGG 1 cut(s) 88
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 3 cut(s) 221, 708, 882
EcoT22I ATGCAT 1 cut(s) 166
Esp3I CGTCTC 1 cut(s) 258
FaeI CATG 1 cut(s) 762
FaqI GGGAC 1 cut(s) 691
FatI CATG 1 cut(s) 758
FauNDI CATATG 1 cut(s) 385
FbaI TGATCA 2 cut(s) 595, 772
Fnu4HI GCNGC 5 cut(s) 198, 359, 381, 458, 477
FokI GGATG 5 cut(s) 151, 515, 566, 725, 890
Fsp4HI GCNGC 5 cut(s) 198, 359, 381, 458, 477
FspBI CTAG 1 cut(s) 402
GlaI GCGC 1 cut(s) 702
GluI GCNGC 5 cut(s) 198, 359, 381, 458, 477
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 1 cut(s) 882
HapII CCGG 1 cut(s) 168
HgaI GACGC 1 cut(s) 14
HhaI GCGC 1 cut(s) 703
Hin1II CATG 1 cut(s) 762
Hin6I GCGC 1 cut(s) 701
HinP1I GCGC 1 cut(s) 701
HinfI GANTC 5 cut(s) 179, 208, 655, 716, 755
HpaII CCGG 1 cut(s) 168
HphI GGTGA 1 cut(s) 650
Hpy188I TCNGA 2 cut(s) 295, 637
Hpy188III TCNNGA 5 cut(s) 146, 275, 421, 749, 776
Hpy99I CGWCG 1 cut(s) 30
HpyAV CCTTC 5 cut(s) 228, 235, 300, 632, 678
HpyCH4III ACNGT 3 cut(s) 142, 436, 541
HpyCH4IV ACGT 1 cut(s) 268
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 170, 269, 731
HpyF3I CTNAG 5 cut(s) 115, 126, 529, 750, 821
HpySE526I ACGT 1 cut(s) 268
Hsp92II CATG 1 cut(s) 762
HspAI GCGC 1 cut(s) 701
Ksp22I TGATCA 2 cut(s) 595, 772
Kzo9I GATC 3 cut(s) 595, 641, 772
LmnI GCTCC 2 cut(s) 390, 739
Lsp1109I GCAGC 4 cut(s) 209, 370, 444, 488
LweI GCATC 4 cut(s) 65, 139, 173, 544
MaeI CTAG 1 cut(s) 402
MaeII ACGT 1 cut(s) 268
MaeIII GTNAC 1 cut(s) 205
MalI GATC 3 cut(s) 597, 643, 774
MboI GATC 3 cut(s) 595, 641, 772
MboII GAAGA 6 cut(s) 51, 54, 57, 103, 238, 250
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 1 cut(s) 641
MhlI GDGCHC 3 cut(s) 175, 274, 434
MluCI AATT 5 cut(s) 4, 201, 588, 600, 692
MlyI GAGTC 3 cut(s) 202, 710, 764
MmeI TCCRAC 1 cut(s) 111
Mph1103I ATGCAT 1 cut(s) 166
MseI TTAA 4 cut(s) 347, 414, 603, 695
MspCI CTTAAG 1 cut(s) 346
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 3 cut(s) 223, 710, 884
MunI CAATTG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 166
MvaI CCWGG 3 cut(s) 223, 710, 884
MwoI GCNNNNNNNGC 4 cut(s) 53, 170, 269, 731
NdeI CATATG 1 cut(s) 385
NdeII GATC 3 cut(s) 595, 641, 772
NlaIII CATG 1 cut(s) 762
NlaIV GGNNCC 3 cut(s) 172, 609, 643
NmuCI GTSAC 1 cut(s) 205
NsiI ATGCAT 1 cut(s) 166
PaeR7I CTCGAG 1 cut(s) 890
PaqCI CACCTGC 1 cut(s) 319
PctI GAATGC 1 cut(s) 166
PfeI GAWTC 2 cut(s) 179, 655
Pfl23II CGTACG 1 cut(s) 545
PfoI TCCNGGA 1 cut(s) 221
PkrI GCNGC 5 cut(s) 199, 360, 382, 459, 478
PleI GAGTC 3 cut(s) 202, 710, 763
PpsI GAGTC 3 cut(s) 202, 710, 763
Psp6I CCWGG 3 cut(s) 221, 708, 882
PspGI CCWGG 3 cut(s) 221, 708, 882
PspLI CGTACG 1 cut(s) 545
PspN4I GGNNCC 3 cut(s) 172, 609, 643
PspPI GGNCC 1 cut(s) 560
PspXI VCTCGAGB 1 cut(s) 890
PstNI CAGNNNCTG 1 cut(s) 482
PsuI RGATCY 1 cut(s) 641
RsaI GTAC 1 cut(s) 547
RsaNI GTAC 1 cut(s) 546
SaqAI TTAA 4 cut(s) 347, 414, 603, 695
SatI GCNGC 5 cut(s) 198, 359, 381, 458, 477
Sau3AI GATC 3 cut(s) 595, 641, 772
Sau96I GGNCC 1 cut(s) 560
SchI GAGTC 3 cut(s) 202, 710, 764
ScrFI CCNGG 3 cut(s) 223, 710, 884
SduI GDGCHC 3 cut(s) 175, 274, 434
SfaNI GCATC 4 cut(s) 65, 139, 173, 544
SfcI CTRYAG 2 cut(s) 783, 856
Sfr274I CTCGAG 1 cut(s) 890
SinI GGWCC 1 cut(s) 560
SlaI CTCGAG 1 cut(s) 890
SmlI CTYRAG 2 cut(s) 346, 890
SmoI CTYRAG 2 cut(s) 346, 890
Sse9I AATT 5 cut(s) 4, 201, 588, 600, 692
SsiI CCGC 1 cut(s) 381
SspMI CTAG 1 cut(s) 402
StyD4I CCNGG 3 cut(s) 221, 708, 882
TaaI ACNGT 3 cut(s) 142, 436, 541
TaiI ACGT 1 cut(s) 271
TaqI TCGA 1 cut(s) 891
TasI AATT 5 cut(s) 4, 201, 588, 600, 692
TauI GCSGC 1 cut(s) 383
TfiI GAWTC 2 cut(s) 179, 655
Tru1I TTAA 4 cut(s) 347, 414, 603, 695
Tru9I TTAA 4 cut(s) 347, 414, 603, 695
TscAI CASTG 2 cut(s) 21, 382
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 4 cut(s) 197, 358, 457, 476
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 17, 173
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 2 cut(s) 21, 382
Vha464I CTTAAG 1 cut(s) 346
VpaK11BI GGWCC 1 cut(s) 560
XagI CCTNNNNNAGG 1 cut(s) 88
XapI RAATTY 1 cut(s) 4
XcmI CCANNNNNNNNNTGG 1 cut(s) 580
XhoI CTCGAG 1 cut(s) 890
XspI CTAG 1 cut(s) 402
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.