Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0009304

Physical Location & Seq

Genomic Coordinates Forward (+)

12611586 .. 12618712

Gene Structure 7127 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00392850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1119 bp

ATGAATTCCTCCTCACTGATGTTGCGTCGGTTCTTGTGCTTCAACGCCTCGCCTCCATCTTCTTCTTCTTCTTCTTCTTCTTCTATTCTATGCTTCTCTTCCAGCAGGAATAGGAAGCCCCTAATCTTCTTAGGCTCTCCTCAGGTCTCTGCAACTGTTCTTGACGCCTTGCTGAATGCATCCGGTGGCCCAGATTCTCTGTTTGAGGTTGCAGCAATTGTGACTCAACCACCTTCCAGGAAGAATAGGGGGAAGAAGGTGCTGCCGTCTCCTTTAGCACAACATGCTCTTGACAGAGGCTTTCCTTCAGAGCTCATTTTCACACCTGCACGAGCTGGAGAGGATATGTTCCTGTCCAGCTTAAGAGCTTTGAAGCCAGAACTTTGTATCACTGCCGCATATGGGAACATTTTACCTAGCAAGTTTCTTAATATCCCGACTTTGGGCACAGTAAATATACACCCAAGTTTGCTGCCGTTGTATCGTGGTGCAGCACCTGTTCAAAGAGCTTTGCAGGATGGTGTCAAAGAAACAGGAGTATCCTTAGCATTTACTGTCCGTGCACTGGATGCTGGACCTGTGATAGCCAGTCAAGGACTGGAAATTGATGATCAAATTAAGGCACCAGATTTGCTTGCTCTCCTATTTTCTGAAGGATCCAAACTATTGATTCACCAGCTTCCTTCCATATTTGATGGTTCAGCTAAATTAAAAGCGCAGTCCCAGGATGACTCTAAAGCTACATTAGCTCCAAAGATTTCTCCTGAGGAGTCATGGCTATCATTTGATCAGGAAGCTGTAGTCCTACATAACAAGGTTCGTGCATTTGCAGGATGGCCTGGAACTCGAGCTAAAGTTGTAGTTGTTGACAACAAAAATGGTCACCGCAATATACTGGAGCTTAAAATTATCACAACCAAAGTTTGCAGCCAAAGCAATATTCATGTCAACCAAGCGGATGACATCTCTTTTCGAAAGGGTGCATTAGTATTCCCCTGTGGTGGGTCCACATCACTTGAGGTGCTGGAGATTCAGCTTCCAGGTAAGAAGGTTATGAATGCAGCTGCCTTCTGGAACGGTTTACGTGGTCAACAACTTAAAAGATTTGTTGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00670 ko00970 map00670 map00970

Pfam Domains

Protein Families

Formyl_trans_C Formyl_trans_N

Protein Analysis

372

Amino Acids

39.99

Weight (kDa)

9.36

Isoelectric Point (pI)

53.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Formyl_trans_N	PF00551	44 - 223	6.1e-31	Formyl transferase
Formyl_trans_C	PF02911	252 - 361	4.3e-17	Formyl transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009304)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66520 AT1G66520
fragaria_vesca	FvH4_2g04020
malus_domestica	MD09G1260600.v1.1
prunus_persica	Prupe.3G152400_v2.0.a1 Prupe.3G152400_v2.0.a1 Prupe.3G152400_v2.0.a1 Prupe.3G152400_v2.0.a1
pyrus_communis	pycom09g17510
rosa_chinensis	RchiOBHm_Chr3g0491591 RchiOBHm_Chr3g0493551
rosa_laevigata	RLG00000022705 RLG00000022838
rosa_multiflora	Rmu_sc0000927.1_g000008
rosa_roxburghii	Rroxscaffold_6G00392850
rosa_rugosa	Rorug03G0250000
rosa_samantha	Rh3AG299300 Rh3AG311200 Rh3BG335300 Rh3BG347100 Rh3CG345200 Rh3DG347400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	334
Acc36I	ACCTGC	1 cut(s)	334
AccB1I	GGYRCC	1 cut(s)	622
AciI	CCGC	3 cut(s)	396, 886, 956
AclWI	GGATC	2 cut(s)	651, 664
AcsI	RAATTY	1 cut(s)	4
AcuI	CTGAAG	2 cut(s)	291, 672
AcyI	GRCGYC	1 cut(s)	165
AfiI	CCNNNNNNNGG	6 cut(s)	402, 442, 443, 565, 1001, 1002
AflII	CTTAAG	1 cut(s)	361
AgsI	TTSAA	3 cut(s)	43, 373, 503
AjnI	CCWGG	4 cut(s)	236, 723, 838, 1039
AjuI	GAANNNNNNNTTGG	2 cut(s)	924, 956
Alw21I	GWGCWC	2 cut(s)	315, 565
Alw26I	GTCTC	2 cut(s)	151, 273
Alw44I	GTGCAC	1 cut(s)	561
AlwI	GGATC	2 cut(s)	651, 664
AlwNI	CAGNNNCTG	1 cut(s)	497
Ama87I	CYCGRG	1 cut(s)	846
AoxI	GGCC	2 cut(s)	187, 836
ApaLI	GTGCAC	1 cut(s)	561
ApeKI	GCWGC	7 cut(s)	212, 262, 472, 491, 927, 1061, 1064
ApoI	RAATTY	1 cut(s)	4
AspLEI	GCGC	1 cut(s)	718
AspS9I	GGNCC	3 cut(s)	188, 575, 1005
AsuHPI	GGTGA	2 cut(s)	665, 875
AsuII	TTCGAA	1 cut(s)	973
AvaI	CYCGRG	1 cut(s)	846
AvaII	GGWCC	2 cut(s)	575, 1005
AxyI	CCTNAGG	2 cut(s)	141, 765
BaeGI	GKGCMC	2 cut(s)	449, 565
BaeI	ACNNNNGTAYC	2 cut(s)	522, 555
BamHI	GGATCC	1 cut(s)	656
BanI	GGYRCC	1 cut(s)	622
BanII	GRGCYC	1 cut(s)	315
BauI	CACGAG	1 cut(s)	330
Bbv12I	GWGCWC	2 cut(s)	315, 565
BbvI	GCAGC	7 cut(s)	224, 249, 459, 503, 939, 1051, 1073
BccI	CCATC	4 cut(s)	64, 512, 689, 828
BceAI	ACGGC	2 cut(s)	250, 460
BciT130I	CCWGG	4 cut(s)	238, 725, 840, 1041
BciVI	GTATCC	1 cut(s)	550
BclI	TGATCA	2 cut(s)	610, 787
BcoDI	GTCTC	2 cut(s)	151, 273
BfaI	CTAG	1 cut(s)	417
BfmI	CTRYAG	1 cut(s)	798
BfrI	CTTAAG	1 cut(s)	361
BfuAI	ACCTGC	1 cut(s)	334
BfuI	GTATCC	1 cut(s)	550
BisI	GCNGC	8 cut(s)	213, 263, 396, 473, 492, 928, 1062, 1065
BlsI	GCNGC	8 cut(s)	214, 264, 397, 474, 493, 929, 1063, 1066
Bme1390I	CCNGG	4 cut(s)	238, 725, 840, 1041
Bme18I	GGWCC	2 cut(s)	575, 1005
BmeT110I	CYCGRG	1 cut(s)	846
BmgT120I	GGNCC	3 cut(s)	188, 575, 1005
BmiI	GGNNCC	3 cut(s)	624, 658, 1006
BmrFI	CCNGG	4 cut(s)	238, 725, 840, 1041
BmsI	GCATC	2 cut(s)	188, 559
BpmI	CTGGAG	3 cut(s)	357, 917, 1046
Bpu10I	CCTNAGC	1 cut(s)	544
Bpu14I	TTCGAA	1 cut(s)	973
BpuEI	CTTGAG	1 cut(s)	1037
BsaAI	YACGTR	1 cut(s)	1085
BsaHI	GRCGYC	1 cut(s)	165
BsaI	GGTCTC	1 cut(s)	151
BsaJI	CCNNGG	1 cut(s)	723
BsaWI	WCCGGW	1 cut(s)	182
BsaXI	ACNNNNNCTCC	2 cut(s)	733, 763
Bsc4I	CCNNNNNNNGG	6 cut(s)	402, 442, 443, 565, 1001, 1002
Bse1I	ACTGG	4 cut(s)	570, 588, 603, 900
Bse21I	CCTNAGG	2 cut(s)	141, 765
BseBI	CCWGG	4 cut(s)	238, 725, 840, 1041
BseDI	CCNNGG	1 cut(s)	723
BseGI	GGATG	6 cut(s)	179, 523, 574, 733, 839, 964
BseLI	CCNNNNNNNGG	6 cut(s)	402, 442, 443, 565, 1001, 1002
BseMII	CTCAG	2 cut(s)	155, 756
BseNI	ACTGG	4 cut(s)	570, 588, 603, 900
BseRI	GAGGAG	2 cut(s)	129, 782
BseSI	GKGCMC	2 cut(s)	449, 565
BseXI	GCAGC	7 cut(s)	224, 249, 459, 503, 939, 1051, 1073
BsgI	GTGCAG	2 cut(s)	312, 510
BshFI	GGCC	2 cut(s)	189, 838
BshNI	GGYRCC	1 cut(s)	622
BsiHKAI	GWGCWC	2 cut(s)	315, 565
BsiHKCI	CYCGRG	1 cut(s)	846
BsiSI	CCGG	1 cut(s)	183
BslFI	GGGAC	1 cut(s)	706
BslI	CCNNNNNNNGG	6 cut(s)	402, 442, 443, 565, 1001, 1002
BsmAI	GTCTC	2 cut(s)	151, 273
BsmBI	CGTCTC	1 cut(s)	273
BsmFI	GGGAC	1 cut(s)	706
BsmI	GAATGC	2 cut(s)	181, 1063
BsnI	GGCC	2 cut(s)	189, 838
Bso31I	GGTCTC	1 cut(s)	151
BsoBI	CYCGRG	1 cut(s)	846
Bsp119I	TTCGAA	1 cut(s)	973
Bsp1286I	GDGCHC	3 cut(s)	315, 449, 565
Bsp143I	GATC	3 cut(s)	610, 656, 787
BspACI	CCGC	3 cut(s)	396, 886, 956
BspANI	GGCC	2 cut(s)	189, 838
BspCNI	CTCAG	2 cut(s)	154, 757
BspLI	GGNNCC	3 cut(s)	624, 658, 1006
BspMI	ACCTGC	1 cut(s)	334
BspPI	GGATC	2 cut(s)	651, 664
BspT104I	TTCGAA	1 cut(s)	973
BspT107I	GGYRCC	1 cut(s)	622
BspTI	CTTAAG	1 cut(s)	361
BspTNI	GGTCTC	1 cut(s)	151
BsrI	ACTGG	4 cut(s)	570, 588, 603, 900
BssECI	CCNNGG	1 cut(s)	723
BssMI	GATC	3 cut(s)	610, 656, 787
BssNI	GRCGYC	1 cut(s)	165
BssSI	CACGAG	1 cut(s)	330
Bst2BI	CACGAG	1 cut(s)	330
Bst2UI	CCWGG	4 cut(s)	238, 725, 840, 1041
Bst4CI	ACNGT	4 cut(s)	157, 451, 556, 1079
Bst6I	CTCTTC	1 cut(s)	103
BstACI	GRCGYC	1 cut(s)	165
BstAFI	CTTAAG	1 cut(s)	361
BstAPI	GCANNNNNTGC	2 cut(s)	284, 569
BstBAI	YACGTR	1 cut(s)	1085
BstBI	TTCGAA	1 cut(s)	973
BstC8I	GCNNGC	1 cut(s)	636
BstDEI	CTNAG	4 cut(s)	130, 141, 544, 765
BstEII	GGTNACC	1 cut(s)	881
BstF5I	GGATG	6 cut(s)	179, 523, 574, 733, 839, 964
BstHHI	GCGC	1 cut(s)	718
BstKTI	GATC	3 cut(s)	613, 659, 790
BstMAI	GTCTC	2 cut(s)	151, 273
BstMBI	GATC	3 cut(s)	610, 656, 787
BstMWI	GCNNNNNNNGC	4 cut(s)	284, 569, 746, 933
BstNI	CCWGG	4 cut(s)	238, 725, 840, 1041
BstNSI	RCATGY	1 cut(s)	287
BstPI	GGTNACC	1 cut(s)	881
BstSCI	CCNGG	4 cut(s)	236, 723, 838, 1039
BstSFI	CTRYAG	1 cut(s)	798
BstSLI	GKGCMC	2 cut(s)	449, 565
BstV1I	GCAGC	7 cut(s)	224, 249, 459, 503, 939, 1051, 1073
BstX2I	RGATCY	1 cut(s)	656
BstYI	RGATCY	1 cut(s)	656
Bsu36I	CCTNAGG	2 cut(s)	141, 765
BsuI	GTATCC	1 cut(s)	550
BsuRI	GGCC	2 cut(s)	189, 838
BtsCI	GGATG	6 cut(s)	179, 523, 574, 733, 839, 964
BtsI	GCAGTG	1 cut(s)	390
BtsIMutI	CAGTG	3 cut(s)	14, 390, 563
BveI	ACCTGC	1 cut(s)	334
Cac8I	GCNNGC	1 cut(s)	636
CaiI	CAGNNNCTG	1 cut(s)	497
CfoI	GCGC	1 cut(s)	718
Cfr13I	GGNCC	3 cut(s)	188, 575, 1005
CseI	GACGC	2 cut(s)	14, 173
CspCI	CAANNNNNGTGG	2 cut(s)	997, 1032
CviAII	CATG	3 cut(s)	284, 774, 944
DdeI	CTNAG	4 cut(s)	130, 141, 544, 765
DpnI	GATC	3 cut(s)	612, 658, 789
DpnII	GATC	3 cut(s)	610, 656, 787
Eam1104I	CTCTTC	1 cut(s)	103
EarI	CTCTTC	1 cut(s)	103
Ecl136II	GAGCTC	1 cut(s)	313
Eco24I	GRGCYC	1 cut(s)	315
Eco31I	GGTCTC	1 cut(s)	151
Eco47I	GGWCC	2 cut(s)	575, 1005
Eco53kI	GAGCTC	1 cut(s)	313
Eco57I	CTGAAG	2 cut(s)	291, 672
Eco81I	CCTNAGG	2 cut(s)	141, 765
Eco88I	CYCGRG	1 cut(s)	846
Eco91I	GGTNACC	1 cut(s)	881
EcoICRI	GAGCTC	1 cut(s)	313
EcoO65I	GGTNACC	1 cut(s)	881
EcoRI	GAATTC	1 cut(s)	4
EcoRII	CCWGG	4 cut(s)	236, 723, 838, 1039
EcoT22I	ATGCAT	1 cut(s)	181
EcoT38I	GRGCYC	1 cut(s)	315
Esp3I	CGTCTC	1 cut(s)	273
FaeI	CATG	3 cut(s)	287, 777, 947
FaqI	GGGAC	1 cut(s)	706
FatI	CATG	3 cut(s)	283, 773, 943
FauNDI	CATATG	1 cut(s)	400
FbaI	TGATCA	2 cut(s)	610, 787
Fnu4HI	GCNGC	8 cut(s)	213, 263, 396, 473, 492, 928, 1062, 1065
FokI	GGATG	6 cut(s)	166, 530, 581, 740, 846, 971
FriOI	GRGCYC	1 cut(s)	315
Fsp4HI	GCNGC	8 cut(s)	213, 263, 396, 473, 492, 928, 1062, 1065
FspBI	CTAG	1 cut(s)	417
GlaI	GCGC	1 cut(s)	717
GluI	GCNGC	8 cut(s)	213, 263, 396, 473, 492, 928, 1062, 1065
GsuI	CTGGAG	3 cut(s)	357, 917, 1046
HaeIII	GGCC	2 cut(s)	189, 838
HapII	CCGG	1 cut(s)	183
HgaI	GACGC	2 cut(s)	14, 173
HhaI	GCGC	1 cut(s)	718
Hin1I	GRCGYC	1 cut(s)	165
Hin1II	CATG	3 cut(s)	287, 777, 947
Hin6I	GCGC	1 cut(s)	716
HinP1I	GCGC	1 cut(s)	716
HincII	GTYRAC	3 cut(s)	868, 949, 1091
HindII	GTYRAC	3 cut(s)	868, 949, 1091
HinfI	GANTC	6 cut(s)	194, 223, 670, 731, 770, 1030
HpaII	CCGG	1 cut(s)	183
HphI	GGTGA	2 cut(s)	665, 875
Hpy166II	GTNNAC	6 cut(s)	563, 868, 949, 1008, 1082, 1091
Hpy188I	TCNGA	2 cut(s)	310, 652
Hpy188III	TCNNGA	6 cut(s)	161, 290, 436, 764, 791, 1072
Hpy8I	GTNNAC	6 cut(s)	563, 868, 949, 1008, 1082, 1091
Hpy99I	CGWCG	1 cut(s)	30
HpyAV	CCTTC	7 cut(s)	243, 250, 315, 647, 693, 1042, 1078
HpyCH4III	ACNGT	4 cut(s)	157, 451, 556, 1079
HpyCH4IV	ACGT	1 cut(s)	1084
HpyF10VI	GCNNNNNNNGC	4 cut(s)	284, 569, 746, 933
HpyF3I	CTNAG	4 cut(s)	130, 141, 544, 765
HpySE526I	ACGT	1 cut(s)	1084
Hsp92I	GRCGYC	1 cut(s)	165
Hsp92II	CATG	3 cut(s)	287, 777, 947
HspAI	GCGC	1 cut(s)	716
Ksp22I	TGATCA	2 cut(s)	610, 787
Kzo9I	GATC	3 cut(s)	610, 656, 787
LmnI	GCTCC	2 cut(s)	754, 898
Lsp1109I	GCAGC	7 cut(s)	224, 249, 459, 503, 939, 1051, 1073
LweI	GCATC	2 cut(s)	188, 559
MaeI	CTAG	1 cut(s)	417
MaeII	ACGT	1 cut(s)	1084
MaeIII	GTNAC	2 cut(s)	220, 881
MalI	GATC	3 cut(s)	612, 658, 789
MboI	GATC	3 cut(s)	610, 656, 787
MfeI	CAATTG	1 cut(s)	216
MflI	RGATCY	1 cut(s)	656
MhlI	GDGCHC	3 cut(s)	315, 449, 565
MluCI	AATT	6 cut(s)	4, 216, 603, 615, 707, 906
MlyI	GAGTC	3 cut(s)	217, 725, 779
Mph1103I	ATGCAT	1 cut(s)	181
MseI	TTAA	6 cut(s)	362, 429, 618, 710, 903, 1098
MspA1I	CMGCKG	1 cut(s)	1064
MspCI	CTTAAG	1 cut(s)	361
MspI	CCGG	1 cut(s)	183
MspR9I	CCNGG	4 cut(s)	238, 725, 840, 1041
MunI	CAATTG	1 cut(s)	216
Mva1269I	GAATGC	2 cut(s)	181, 1063
MvaI	CCWGG	4 cut(s)	238, 725, 840, 1041
MwoI	GCNNNNNNNGC	4 cut(s)	284, 569, 746, 933
NdeI	CATATG	1 cut(s)	400
NdeII	GATC	3 cut(s)	610, 656, 787
NlaIII	CATG	3 cut(s)	287, 777, 947
NlaIV	GGNNCC	3 cut(s)	624, 658, 1006
NmuCI	GTSAC	2 cut(s)	220, 881
NsiI	ATGCAT	1 cut(s)	181
NspI	RCATGY	1 cut(s)	287
NspV	TTCGAA	1 cut(s)	973
PaeR7I	CTCGAG	1 cut(s)	846
PaqCI	CACCTGC	1 cut(s)	334
PctI	GAATGC	2 cut(s)	181, 1063
PfeI	GAWTC	3 cut(s)	194, 670, 1030
PfoI	TCCNGGA	1 cut(s)	236
PkrI	GCNGC	8 cut(s)	214, 264, 397, 474, 493, 929, 1063, 1066
PleI	GAGTC	3 cut(s)	217, 725, 778
PpsI	GAGTC	3 cut(s)	217, 725, 778
Ppu21I	YACGTR	1 cut(s)	1085
Psp124BI	GAGCTC	1 cut(s)	315
Psp6I	CCWGG	4 cut(s)	236, 723, 838, 1039
PspEI	GGTNACC	1 cut(s)	881
PspGI	CCWGG	4 cut(s)	236, 723, 838, 1039
PspN4I	GGNNCC	3 cut(s)	624, 658, 1006
PspPI	GGNCC	3 cut(s)	188, 575, 1005
PspXI	VCTCGAGB	1 cut(s)	846
PstNI	CAGNNNCTG	1 cut(s)	497
PsuI	RGATCY	1 cut(s)	656
PvuII	CAGCTG	1 cut(s)	1064
SacI	GAGCTC	1 cut(s)	315
SaqAI	TTAA	6 cut(s)	362, 429, 618, 710, 903, 1098
SatI	GCNGC	8 cut(s)	213, 263, 396, 473, 492, 928, 1062, 1065
Sau3AI	GATC	3 cut(s)	610, 656, 787
Sau96I	GGNCC	3 cut(s)	188, 575, 1005
SchI	GAGTC	3 cut(s)	217, 725, 779
ScrFI	CCNGG	4 cut(s)	238, 725, 840, 1041
SduI	GDGCHC	3 cut(s)	315, 449, 565
SfaNI	GCATC	2 cut(s)	188, 559
SfcI	CTRYAG	1 cut(s)	798
Sfr274I	CTCGAG	1 cut(s)	846
SfuI	TTCGAA	1 cut(s)	973
SinI	GGWCC	2 cut(s)	575, 1005
SlaI	CTCGAG	1 cut(s)	846
SmlI	CTYRAG	3 cut(s)	361, 846, 1016
SmoI	CTYRAG	3 cut(s)	361, 846, 1016
Sse9I	AATT	6 cut(s)	4, 216, 603, 615, 707, 906
SsiI	CCGC	3 cut(s)	396, 886, 956
SspI	AATATT	1 cut(s)	940
SspMI	CTAG	1 cut(s)	417
SstI	GAGCTC	1 cut(s)	315
StyD4I	CCNGG	4 cut(s)	236, 723, 838, 1039
TaaI	ACNGT	4 cut(s)	157, 451, 556, 1079
TaiI	ACGT	1 cut(s)	1087
TaqI	TCGA	2 cut(s)	847, 973
TasI	AATT	6 cut(s)	4, 216, 603, 615, 707, 906
TauI	GCSGC	1 cut(s)	398
TfiI	GAWTC	3 cut(s)	194, 670, 1030
Tru1I	TTAA	6 cut(s)	362, 429, 618, 710, 903, 1098
Tru9I	TTAA	6 cut(s)	362, 429, 618, 710, 903, 1098
TscAI	CASTG	3 cut(s)	21, 397, 570
TseFI	GTSAC	2 cut(s)	220, 881
TseI	GCWGC	7 cut(s)	212, 262, 472, 491, 927, 1061, 1064
Tsp45I	GTSAC	2 cut(s)	220, 881
TspDTI	ATGAA	3 cut(s)	17, 932, 1070
TspGWI	ACGGA	1 cut(s)	548
TspRI	CASTG	3 cut(s)	21, 397, 570
Vha464I	CTTAAG	1 cut(s)	361
VneI	GTGCAC	1 cut(s)	561
VpaK11BI	GGWCC	2 cut(s)	575, 1005
XapI	RAATTY	1 cut(s)	4
XceI	RCATGY	1 cut(s)	287
XcmI	CCANNNNNNNNNTGG	1 cut(s)	595
XhoI	CTCGAG	1 cut(s)	846
XspI	CTAG	1 cut(s)	417
Zsp2I	ATGCAT	1 cut(s)	181

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.