AT1G68820

Transmembrane Fragile-X-F protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004923
Physical Location & Seq
Forward (+)
25865471 .. 25869029
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68820.3

Sequence Viewer

Length: 1407 bp
ATGTTGGTACAGAGAAGAGTAATGAGTTGGAGAAGAGTCTGGAAGTCGTTTCAAGCGGCTTCTGCGCATTGTTTACTCTTCTCCTTCACGCTTCTTCTCGCTTTGAAGCTTGATCATGTTGTTTCTCATTCATGGTGGTTTGTATTTGCACCCTTGTGGTTGTTTCATGCTGTGATTGCTCGTGGTAGATTTTCATTGCCTGCTCCATCAATGCCTCATGATCGACATTGGGCTCCTTTTCACTCGGTTATGGCAACACCACTTCTTGTTGCTTTTGAGATCCTTCTCTGTGTACATCTTGAAGATAAATATGTTGTTGACTTAAAGATTGTCTTTCTACCGTTGCTTGCATTTGAGGTAGCAATTTTGATAGATAATGTCAGAATGTGCAGGACGCTCATGCCTGGAGATGAAGAAACTATGAGTGATGAAGCCATATGGGAAACACTTCCTCATTTCTGGGTTTCCATATCTATGGTTTTCTTCATTGCCGCAACAACCTTCACTCTTCTTAAATTATGTGGTGACGTAGCTGCGTTGGGATGGTGGGACTTATTTATAAACTTCGGAATAGCAGAGTGCTTTGCGTTTCTTGTCTGTACAAAGTGGAGCAATCAGTCAATTCATAGGTATTCACATATACCGGAACCTAGCTCATCTTCAATGGTAGTAAGATATCTGGATTGGAACAGAGGTCTAGTAGTAACCGCTGACGACGAGCATCAGCAAAGCAACAGAATATGTGGTCTCCAAGATATTGGTGGACATGTTATGAAAATTCCATTTGTGACCTTTCAAATCATCCTTTTCATGCGCTTAGAGGGAACGCCAGCTTCTGCCAAAAACATTCCGATTTTAGTTCTGTTTGTACCTCTTTTTCTGTTACAAGGAGCTGGGGTACTTTTTGCTATGTATAGATTGGTTGAGAAATCAGTCTTATTAATAAATAGTGGTAGTGGTTCTTATGGAAGATATTTTACCGCAACATCATCAGCTCGTGAATTCCTGGGATTCTTTCAACATGGTGCAAGATTACTTGGCTGGTGGTCTATCGATGAAGGAAGTCGGGAAGAACAAGCAAGGCTCTACTCTGGAGAAGCTACTGGATACAACACCTTCTCACCAGAGGTTGTGAAGAAAATGCCAAAGTCTGATCTTGTTGAAGAGATATGGAGACTTCAAGCTGCATTGAGTGAGCAAACAGATATCACCAGTTATAGCCAGCAAGAGTACGAAAGGCTTCAAAACGAGAAGATTCTTTGTAGAGTTTGCTTTGAAGATCCGATCAACGTGGTTCTACTCCCATGTAGACATCACGTCCTCTGCAGTACATGCTGCGAGAAATGCAAGAAATGTCCGATTTGTCGTGTCCTGATCGAGGAGCGTATGCCTGTATACGATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_185A zf-C3HC4_3

Protein Analysis

468

Amino Acids

53.64

Weight (kDa)

6.47

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_185A PF10269 8 - 258 4.8e-94 Transmembrane Fragile-X-F protein
zf-C3HC4_3 PF13920 382 - 424 3.6e-11 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
Acc16I TGCGCA 1 cut(s) 66
AccI GTMKAC 2 cut(s) 1309, 1395
AciI CCGC 4 cut(s) 56, 492, 708, 981
AclWI GGATC 2 cut(s) 274, 1274
AcsI RAATTY 2 cut(s) 777, 1001
AfaI GTAC 7 cut(s) 9, 294, 601, 870, 900, 1232, 1330
AfiI CCNNNNNNNGG 1 cut(s) 1378
AflIII ACRYGT 1 cut(s) 766
AhdI GACNNNNNGTC 1 cut(s) 1316
AjiI CACGTC 1 cut(s) 1318
AjnI CCWGG 2 cut(s) 403, 1005
AleI CACNNNNGTG 1 cut(s) 154
AluBI AGCT 8 cut(s) 109, 533, 654, 833, 893, 995, 1100, 1184
AluI AGCT 8 cut(s) 109, 533, 654, 833, 893, 995, 1100, 1184
Alw26I GTCTC 2 cut(s) 752, 1168
AlwI GGATC 2 cut(s) 274, 1274
AlwNI CAGNNNCTG 1 cut(s) 836
ApeKI GCWGC 3 cut(s) 533, 1184, 1335
ApoI RAATTY 2 cut(s) 777, 1001
AseI ATTAAT 1 cut(s) 941
Asp700I GAANNNNTTC 2 cut(s) 447, 1239
AspLEI GCGC 2 cut(s) 67, 816
AsuHPI GGTGA 3 cut(s) 536, 1113, 1201
BanII GRGCYC 1 cut(s) 235
BarI GAAGNNNNNNTAC 2 cut(s) 961, 993
BauI CACGAG 2 cut(s) 180, 996
BbvI GCAGC 3 cut(s) 520, 1171, 1322
BccI CCATC 2 cut(s) 214, 537
BciT130I CCWGG 2 cut(s) 405, 1007
BciVI GTATCC 1 cut(s) 1100
BclI TGATCA 1 cut(s) 112
BcoDI GTCTC 2 cut(s) 752, 1168
BfaI CTAG 2 cut(s) 651, 698
BfmI CTRYAG 1 cut(s) 1324
BfuI GTATCC 1 cut(s) 1100
BisI GCNGC 5 cut(s) 57, 492, 534, 1185, 1336
BlsI GCNGC 5 cut(s) 58, 493, 535, 1186, 1337
Bme1390I CCNGG 2 cut(s) 405, 1007
BmeRI GACNNNNNGTC 1 cut(s) 1316
BmgBI CACGTC 1 cut(s) 1318
BmiI GGNNCC 2 cut(s) 234, 648
BmrFI CCNGG 2 cut(s) 405, 1007
BmsI GCATC 1 cut(s) 730
BpmI CTGGAG 2 cut(s) 426, 1113
Bsa29I ATCGAT 1 cut(s) 1053
BsaI GGTCTC 1 cut(s) 752
BsaJI CCNNGG 1 cut(s) 1006
BsaWI WCCGGW 1 cut(s) 643
BsaXI ACNNNNNCTCC 2 cut(s) 882, 912
Bsc4I CCNNNNNNNGG 1 cut(s) 1378
Bse1I ACTGG 2 cut(s) 1108, 1212
Bse3DI GCAATG 2 cut(s) 194, 486
BseBI CCWGG 2 cut(s) 405, 1007
BseCI ATCGAT 1 cut(s) 1053
BseDI CCNNGG 1 cut(s) 1006
BseGI GGATG 2 cut(s) 548, 801
BseLI CCNNNNNNNGG 1 cut(s) 1378
BseMI GCAATG 2 cut(s) 194, 486
BseNI ACTGG 2 cut(s) 1108, 1212
BseRI GAGGAG 1 cut(s) 1394
BseXI GCAGC 3 cut(s) 520, 1171, 1322
BseYI CCCAGC 1 cut(s) 893
BsgI GTGCAG 1 cut(s) 409
BshVI ATCGAT 1 cut(s) 1053
BsiSI CCGG 1 cut(s) 644
BslFI GGGAC 1 cut(s) 563
BslI CCNNNNNNNGG 1 cut(s) 1378
BsmAI GTCTC 2 cut(s) 752, 1168
BsmFI GGGAC 1 cut(s) 563
Bso31I GGTCTC 1 cut(s) 752
Bsp1286I GDGCHC 1 cut(s) 235
Bsp1407I TGTACA 2 cut(s) 292, 599
Bsp143I GATC 7 cut(s) 112, 220, 279, 1153, 1279, 1284, 1374
BspACI CCGC 4 cut(s) 56, 492, 708, 981
BspDI ATCGAT 1 cut(s) 1053
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 2 cut(s) 234, 648
BspMAI CTGCAG 1 cut(s) 1328
BspPI GGATC 2 cut(s) 274, 1274
BspTNI GGTCTC 1 cut(s) 752
BsrDI GCAATG 2 cut(s) 194, 486
BsrGI TGTACA 2 cut(s) 292, 599
BsrI ACTGG 2 cut(s) 1108, 1212
BssECI CCNNGG 1 cut(s) 1006
BssMI GATC 7 cut(s) 112, 220, 279, 1153, 1279, 1284, 1374
BssNAI GTATAC 1 cut(s) 1396
BssSI CACGAG 2 cut(s) 180, 996
Bst1107I GTATAC 1 cut(s) 1396
Bst2BI CACGAG 2 cut(s) 180, 996
Bst2UI CCWGG 2 cut(s) 405, 1007
Bst4CI ACNGT 1 cut(s) 342
Bst6I CTCTTC 5 cut(s) 10, 28, 83, 513, 1158
BstAPI GCANNNNNTGC 1 cut(s) 1332
BstAUI TGTACA 2 cut(s) 292, 599
BstC8I GCNNGC 4 cut(s) 201, 348, 831, 1223
BstDEI CTNAG 1 cut(s) 817
BstENI CCTNNNNNAGG 1 cut(s) 1376
BstF5I GGATG 2 cut(s) 548, 801
BstHHI GCGC 2 cut(s) 67, 816
BstKTI GATC 7 cut(s) 115, 223, 282, 1156, 1282, 1287, 1377
BstMAI GTCTC 2 cut(s) 752, 1168
BstMBI GATC 7 cut(s) 112, 220, 279, 1153, 1279, 1284, 1374
BstMWI GCNNNNNNNGC 4 cut(s) 62, 176, 1332, 1344
BstNI CCWGG 2 cut(s) 405, 1007
BstNSI RCATGY 2 cut(s) 770, 1335
BstSCI CCNGG 2 cut(s) 403, 1005
BstSFI CTRYAG 1 cut(s) 1324
BstV1I GCAGC 3 cut(s) 520, 1171, 1322
BstX2I RGATCY 2 cut(s) 279, 1279
BstXI CCANNNNNNTGG 2 cut(s) 475, 758
BstYI RGATCY 2 cut(s) 279, 1279
BstZ17I GTATAC 1 cut(s) 1396
Bsu15I ATCGAT 1 cut(s) 1053
BsuI GTATCC 1 cut(s) 1100
BsuTUI ATCGAT 1 cut(s) 1053
BtrI CACGTC 1 cut(s) 1318
BtsCI GGATG 2 cut(s) 548, 801
Cac8I GCNNGC 4 cut(s) 201, 348, 831, 1223
CaiI CAGNNNCTG 1 cut(s) 836
CciI TCATGA 1 cut(s) 217
CfoI GCGC 2 cut(s) 67, 816
ClaI ATCGAT 1 cut(s) 1053
CseI GACGC 1 cut(s) 403
Csp6I GTAC 7 cut(s) 8, 293, 600, 869, 899, 1231, 1329
CviQI GTAC 7 cut(s) 8, 293, 600, 869, 899, 1231, 1329
DdeI CTNAG 1 cut(s) 817
DpnI GATC 7 cut(s) 114, 222, 281, 1155, 1281, 1286, 1376
DpnII GATC 7 cut(s) 112, 220, 279, 1153, 1279, 1284, 1374
DriI GACNNNNNGTC 1 cut(s) 1316
Eam1104I CTCTTC 5 cut(s) 10, 28, 83, 513, 1158
Eam1105I GACNNNNNGTC 1 cut(s) 1316
EarI CTCTTC 5 cut(s) 10, 28, 83, 513, 1158
Eco24I GRGCYC 1 cut(s) 235
Eco31I GGTCTC 1 cut(s) 752
Eco32I GATATC 2 cut(s) 677, 1207
EcoNI CCTNNNNNAGG 1 cut(s) 1376
EcoRI GAATTC 1 cut(s) 1001
EcoRII CCWGG 2 cut(s) 403, 1005
EcoRV GATATC 2 cut(s) 677, 1207
EcoT38I GRGCYC 1 cut(s) 235
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 1 cut(s) 563
FauNDI CATATG 1 cut(s) 437
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 2 cut(s) 1309, 1395
Fnu4HI GCNGC 5 cut(s) 57, 492, 534, 1185, 1336
FokI GGATG 2 cut(s) 555, 788
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 5 cut(s) 57, 492, 534, 1185, 1336
FspBI CTAG 2 cut(s) 651, 698
FspI TGCGCA 1 cut(s) 66
GlaI GCGC 2 cut(s) 66, 815
GluI GCNGC 5 cut(s) 57, 492, 534, 1185, 1336
GsaI CCCAGC 1 cut(s) 897
GsuI CTGGAG 2 cut(s) 426, 1113
HapII CCGG 1 cut(s) 644
HgaI GACGC 1 cut(s) 403
HhaI GCGC 2 cut(s) 67, 816
Hin6I GCGC 2 cut(s) 65, 814
HinP1I GCGC 2 cut(s) 65, 814
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 3 cut(s) 36, 1011, 1255
HpaII CCGG 1 cut(s) 644
HphI GGTGA 3 cut(s) 536, 1113, 1201
Hpy166II GTNNAC 6 cut(s) 74, 293, 319, 764, 1310, 1396
Hpy188I TCNGA 6 cut(s) 383, 569, 852, 1153, 1284, 1359
Hpy188III TCNNGA 8 cut(s) 40, 218, 299, 680, 998, 1067, 1092, 1372
Hpy8I GTNNAC 6 cut(s) 74, 293, 319, 764, 1310, 1396
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 5 cut(s) 94, 293, 511, 1052, 1126
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 3 cut(s) 528, 1290, 1317
HpyCH4V TGCA 7 cut(s) 149, 350, 390, 1028, 1187, 1326, 1347
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 176, 1332, 1344
HpyF3I CTNAG 1 cut(s) 817
HpySE526I ACGT 3 cut(s) 528, 1290, 1317
HspAI GCGC 2 cut(s) 65, 814
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 7 cut(s) 112, 220, 279, 1153, 1279, 1284, 1374
LmnI GCTCC 5 cut(s) 208, 238, 609, 890, 1381
Lsp1109I GCAGC 3 cut(s) 520, 1171, 1322
LweI GCATC 1 cut(s) 730
MaeI CTAG 2 cut(s) 651, 698
MaeII ACGT 3 cut(s) 528, 1290, 1317
MaeIII GTNAC 4 cut(s) 524, 703, 787, 882
MalI GATC 7 cut(s) 114, 222, 281, 1155, 1281, 1286, 1376
MboI GATC 7 cut(s) 112, 220, 279, 1153, 1279, 1284, 1374
MflI RGATCY 2 cut(s) 279, 1279
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 5 cut(s) 363, 515, 621, 777, 1001
MlyI GAGTC 1 cut(s) 45
MmeI TCCRAC 1 cut(s) 8
MnlI CCTC 9 cut(s) 225, 349, 462, 686, 814, 882, 1120, 1331, 1372
MroXI GAANNNNTTC 2 cut(s) 447, 1239
MseI TTAA 3 cut(s) 323, 513, 941
MslI CAYNNNNRTG 2 cut(s) 154, 473
MspA1I CMGCKG 1 cut(s) 710
MspI CCGG 1 cut(s) 644
MspR9I CCNGG 2 cut(s) 405, 1007
MvaI CCWGG 2 cut(s) 405, 1007
MwoI GCNNNNNNNGC 4 cut(s) 62, 176, 1332, 1344
NdeI CATATG 1 cut(s) 437
NdeII GATC 7 cut(s) 112, 220, 279, 1153, 1279, 1284, 1374
NlaIV GGNNCC 2 cut(s) 234, 648
NmuCI GTSAC 2 cut(s) 524, 787
NsbI TGCGCA 1 cut(s) 66
NspI RCATGY 2 cut(s) 770, 1335
OliI CACNNNNGTG 1 cut(s) 154
PagI TCATGA 1 cut(s) 217
PciI ACATGT 1 cut(s) 766
PdmI GAANNNNTTC 2 cut(s) 447, 1239
PfeI GAWTC 2 cut(s) 1011, 1255
PkrI GCNGC 5 cut(s) 58, 493, 535, 1186, 1337
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
PscI ACATGT 1 cut(s) 766
PshBI ATTAAT 1 cut(s) 941
PsiI TTATAA 1 cut(s) 560
Psp6I CCWGG 2 cut(s) 403, 1005
PspFI CCCAGC 1 cut(s) 893
PspGI CCWGG 2 cut(s) 403, 1005
PspN4I GGNNCC 2 cut(s) 234, 648
PstI CTGCAG 1 cut(s) 1328
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 2 cut(s) 279, 1279
RsaI GTAC 7 cut(s) 9, 294, 601, 870, 900, 1232, 1330
RsaNI GTAC 7 cut(s) 8, 293, 600, 869, 899, 1231, 1329
RseI CAYNNNNRTG 2 cut(s) 154, 473
SaqAI TTAA 3 cut(s) 323, 513, 941
SatI GCNGC 5 cut(s) 57, 492, 534, 1185, 1336
Sau3AI GATC 7 cut(s) 112, 220, 279, 1153, 1279, 1284, 1374
SchI GAGTC 1 cut(s) 45
ScrFI CCNGG 2 cut(s) 405, 1007
SduI GDGCHC 1 cut(s) 235
SfaNI GCATC 1 cut(s) 730
SfcI CTRYAG 1 cut(s) 1324
SmiMI CAYNNNNRTG 2 cut(s) 154, 473
Sse9I AATT 5 cut(s) 363, 515, 621, 777, 1001
SsiI CCGC 4 cut(s) 56, 492, 708, 981
SspMI CTAG 2 cut(s) 651, 698
StyD4I CCNGG 2 cut(s) 403, 1005
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 3 cut(s) 531, 1293, 1320
TaqI TCGA 3 cut(s) 223, 1053, 1377
TasI AATT 5 cut(s) 363, 515, 621, 777, 1001
TatI WGTACW 3 cut(s) 292, 599, 1328
TauI GCSGC 2 cut(s) 59, 494
TfiI GAWTC 2 cut(s) 1011, 1255
Tru1I TTAA 3 cut(s) 323, 513, 941
Tru9I TTAA 3 cut(s) 323, 513, 941
TseFI GTSAC 2 cut(s) 524, 787
TseI GCWGC 3 cut(s) 533, 1184, 1335
Tsp45I GTSAC 2 cut(s) 524, 787
VspI ATTAAT 1 cut(s) 941
XagI CCTNNNNNAGG 1 cut(s) 1376
XapI RAATTY 2 cut(s) 777, 1001
XceI RCATGY 2 cut(s) 770, 1335
XcmI CCANNNNNNNNNTGG 1 cut(s) 758
XmiI GTMKAC 2 cut(s) 1309, 1395
XmnI GAANNNNTTC 2 cut(s) 447, 1239
XspI CTAG 2 cut(s) 651, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.