Rh4AG344800

Transmembrane Fragile-X-F protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004923
Physical Location & Seq
Reverse (-)
65318042 .. 65323845
5804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG344800.1

Sequence Viewer

Length: 1224 bp
ATGTTGGTAGAGCGGAGAGGGATGACGTGGCGCTCCGTGGCCAAGTCCCTGCAAGCTCTTGTGGCGCATGCCCTGCTTTCCACATTCACCGTTTTCCTCGTCCTCCGCCTCCACCACGCCGTCCGCTACCACTGGTGGATCATATTCTCCCCTCTGTGGCTTTTCCATGCAGTTGTAGCAAGAGGCAGATTCTCATTACCAGCTCCAGTGCCCCCTAATGATCGTCACTGGGCGCCACTTCATGCTGTCACGGCAACCCCCTTGCTTGTTGCGTTTGAACTACTGCTTTGTATATATTTAGAGAACAGATACGTTGTAAGCTTGAAGATTGTCTTTGTGCCATTGTTGGCATTTGAACTAGCAATTTTATTTGACAATGTCAGGATGTGTAAGGCTCTAATGCCTAGTGATGATGACACCATGAGTGATGATGTGATATGGGAAACTCTTCCTCACTTCTGGATTTCTATATCTATGGTCTTCCTTATTGCTGCCACAACATTCACTCTTCTAAAGATAAATGGTGATATTTCTGCTCTAGGCTGGTGGGACTTATTTATAAACTTTGGCATTGCACAGATGTTTGCCTTTCTTGTTTGCACGAAGTGGTATAACCCAGCAATTCACAGACATGCTCACTCTGGAGAAGCATGTTCATCTTCAGTGACCATAAGATATCTTGATTGGAATAGAGGCTTTGTAGTTTCTTCAGAAGAAGACCAAGAAGAGAGTGGAATATGCAGCCTGCAGGATATTGGTGGACATATTATGAAAATTCCTCTAATCACTTTCCAGATTCTGCTGTTTATGCGCTTAGAGGGAACACCATCCGGTGCTAGTCAAATCTCAATTCCACTTCTTTTTGCTCCTCTTCTTTTACTGCAAGGAGCTGGTGTTTTATATGCTGCATATAGGTTGATAGAGAAAATTGTACTTTTAGTACGTAGTGGAGTTGTTTCTGAAAGATACTTGGTCATAACATCAAAAGTTCGTGATTATTTTGGGTTCCTGCGTCATGGTTCTAGGCTTCTGGGTTGGTGGTCAATTGATGAAGGAAGTCGAGAGGAACAAGCTAGACTGTATTCTGCCGGAGCTTCAGGGTCTGTTTTTCCTGTACTCAGTACTTTCATTTACTTCATTTATCTTCTGCAAATTTTAGGCAATCTTCTTCTGTGTCCTTTTGCTTTTTTATTGAAACAAGTCCTCATCAATGTAATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_185A zf-C3HC4_3

Protein Analysis

407

Amino Acids

46.11

Weight (kDa)

7.72

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_185A PF10269 35 - 294 6.6e-91 Transmembrane Fragile-X-F protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AccB1I GGYRCC 1 cut(s) 232
AccBSI CCGCTC 1 cut(s) 13
AciI CCGC 3 cut(s) 13, 106, 124
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 2 cut(s) 774, 1152
AcuI CTGAAG 3 cut(s) 645, 693, 1080
AcyI GRCGYC 1 cut(s) 233
AdeI CACNNNGTG 1 cut(s) 606
AfaI GTAC 4 cut(s) 933, 942, 1116, 1123
AfiI CCNNNNNNNGG 1 cut(s) 156
AgsI TTSAA 4 cut(s) 278, 325, 356, 1195
AjiI CACGTC 1 cut(s) 27
AluBI AGCT 6 cut(s) 56, 203, 321, 890, 1073, 1094
AluI AGCT 6 cut(s) 56, 203, 321, 890, 1073, 1094
AlwI GGATC 1 cut(s) 146
AlwNI CAGNNNCTG 2 cut(s) 799, 1103
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 3 cut(s) 491, 741, 905
ApoI RAATTY 2 cut(s) 774, 1152
Asp700I GAANNNNTTC 1 cut(s) 447
AspLEI GCGC 4 cut(s) 33, 67, 235, 813
AsuHPI GGTGA 2 cut(s) 79, 536
BaeGI GKGCMC 1 cut(s) 213
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 232
BbsI GAAGAC 2 cut(s) 472, 723
BbvI GCAGC 3 cut(s) 478, 753, 892
BccI CCATC 1 cut(s) 835
BceAI ACGGC 2 cut(s) 104, 267
BfaI CTAG 6 cut(s) 359, 405, 539, 837, 1023, 1074
BfmI CTRYAG 1 cut(s) 746
BfoI RGCGCY 2 cut(s) 34, 236
BisI GCNGC 3 cut(s) 492, 742, 906
BlsI GCNGC 3 cut(s) 493, 743, 907
BmcAI AGTACT 1 cut(s) 1123
BmgBI CACGTC 1 cut(s) 27
BmiI GGNNCC 2 cut(s) 234, 1007
BmrI ACTGGG 1 cut(s) 238
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 2 cut(s) 472, 723
BpmI CTGGAG 2 cut(s) 189, 663
BsaAI YACGTR 1 cut(s) 944
BsaHI GRCGYC 1 cut(s) 233
BsaJI CCNNGG 1 cut(s) 36
BsaWI WCCGGW 1 cut(s) 830
BsaXI ACNNNNNCTCC 2 cut(s) 879, 909
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 3 cut(s) 137, 206, 233
Bse3DI GCAATG 1 cut(s) 570
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 3 cut(s) 27, 390, 827
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 1 cut(s) 1132
BseNI ACTGG 3 cut(s) 137, 206, 233
BseRI GAGGAG 1 cut(s) 858
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 3 cut(s) 478, 753, 892
BseYI CCCAGC 1 cut(s) 616
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 1 cut(s) 232
BsiSI CCGG 2 cut(s) 831, 1089
BslFI GGGAC 2 cut(s) 31, 563
BslI CCNNNNNNNGG 1 cut(s) 156
BsmFI GGGAC 2 cut(s) 31, 563
BsnI GGCC 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 2 cut(s) 138, 220
BspACI CCGC 3 cut(s) 13, 106, 124
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 1 cut(s) 1131
BspLI GGNNCC 2 cut(s) 234, 1007
BspMAI CTGCAG 1 cut(s) 750
BspPI GGATC 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 232
BsrBI CCGCTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 3 cut(s) 137, 206, 233
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 2 cut(s) 138, 220
BssNI GRCGYC 1 cut(s) 233
Bst4CI ACNGT 2 cut(s) 91, 1080
Bst6I CTCTTC 4 cut(s) 453, 513, 720, 876
BstACI GRCGYC 1 cut(s) 233
BstAPI GCANNNNNTGC 1 cut(s) 73
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 3 cut(s) 54, 69, 746
BstDEI CTNAG 2 cut(s) 814, 1118
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 3 cut(s) 27, 390, 827
BstH2I RGCGCY 2 cut(s) 34, 236
BstHHI GCGC 4 cut(s) 33, 67, 235, 813
BstKTI GATC 2 cut(s) 141, 223
BstMBI GATC 2 cut(s) 138, 220
BstMWI GCNNNNNNNGC 5 cut(s) 62, 73, 176, 251, 808
BstNSI RCATGY 3 cut(s) 71, 635, 654
BstSFI CTRYAG 1 cut(s) 746
BstSLI GKGCMC 1 cut(s) 213
BstSNI TACGTA 1 cut(s) 944
BstV1I GCAGC 3 cut(s) 478, 753, 892
BstV2I GAAGAC 2 cut(s) 472, 723
BsuRI GGCC 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 36
BtrI CACGTC 1 cut(s) 27
BtsCI GGATG 3 cut(s) 27, 390, 827
BtsIMutI CAGTG 4 cut(s) 130, 213, 226, 669
Cac8I GCNNGC 3 cut(s) 54, 69, 746
CaiI CAGNNNCTG 2 cut(s) 799, 1103
CfoI GCGC 4 cut(s) 33, 67, 235, 813
CseI GACGC 1 cut(s) 1001
Csp6I GTAC 4 cut(s) 932, 941, 1115, 1122
CviAII CATG 7 cut(s) 68, 167, 242, 421, 632, 651, 1016
CviQI GTAC 4 cut(s) 932, 941, 1115, 1122
DdeI CTNAG 2 cut(s) 814, 1118
DinI GGCGCC 1 cut(s) 234
DpnI GATC 2 cut(s) 140, 222
DpnII GATC 2 cut(s) 138, 220
DraIII CACNNNGTG 1 cut(s) 606
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 4 cut(s) 453, 513, 720, 876
EarI CTCTTC 4 cut(s) 453, 513, 720, 876
EciI GGCGGA 1 cut(s) 95
Eco105I TACGTA 1 cut(s) 944
Eco32I GATATC 1 cut(s) 677
Eco57I CTGAAG 3 cut(s) 645, 693, 1080
EcoRV GATATC 1 cut(s) 677
EgeI GGCGCC 1 cut(s) 234
EheI GGCGCC 1 cut(s) 234
FaeI CATG 7 cut(s) 71, 170, 245, 424, 635, 654, 1019
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 2 cut(s) 31, 563
FatI CATG 7 cut(s) 67, 166, 241, 420, 631, 650, 1015
Fnu4HI GCNGC 3 cut(s) 492, 742, 906
FokI GGATG 3 cut(s) 34, 397, 814
Fsp4HI GCNGC 3 cut(s) 492, 742, 906
FspBI CTAG 6 cut(s) 359, 405, 539, 837, 1023, 1074
GlaI GCGC 4 cut(s) 32, 66, 234, 812
GluI GCNGC 3 cut(s) 492, 742, 906
GsaI CCCAGC 1 cut(s) 620
GsuI CTGGAG 2 cut(s) 189, 663
HaeII RGCGCY 2 cut(s) 34, 236
HaeIII GGCC 1 cut(s) 41
HapII CCGG 2 cut(s) 831, 1089
HgaI GACGC 1 cut(s) 1001
HhaI GCGC 4 cut(s) 33, 67, 235, 813
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 7 cut(s) 71, 170, 245, 424, 635, 654, 1019
Hin6I GCGC 4 cut(s) 31, 65, 233, 811
HinP1I GCGC 4 cut(s) 31, 65, 233, 811
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 2 cut(s) 189, 796
HpaII CCGG 2 cut(s) 831, 1089
HphI GGTGA 2 cut(s) 79, 536
Hpy166II GTNNAC 1 cut(s) 761
Hpy188I TCNGA 2 cut(s) 712, 961
Hpy188III TCNNGA 8 cut(s) 382, 460, 642, 680, 793, 992, 1061, 1221
Hpy8I GTNNAC 1 cut(s) 761
HpyAV CCTTC 1 cut(s) 1046
HpyCH4III ACNGT 2 cut(s) 91, 1080
HpyCH4IV ACGT 3 cut(s) 26, 312, 943
HpyCH4V TGCA 9 cut(s) 52, 170, 575, 600, 741, 748, 883, 908, 1150
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 73, 176, 251, 808
HpyF3I CTNAG 2 cut(s) 814, 1118
HpySE526I ACGT 3 cut(s) 26, 312, 943
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 7 cut(s) 71, 170, 245, 424, 635, 654, 1019
HspAI GCGC 4 cut(s) 31, 65, 233, 811
KasI GGCGCC 1 cut(s) 232
Kzo9I GATC 2 cut(s) 138, 220
LmnI GCTCC 5 cut(s) 38, 208, 871, 887, 1091
Lsp1109I GCAGC 3 cut(s) 478, 753, 892
MaeI CTAG 6 cut(s) 359, 405, 539, 837, 1023, 1074
MaeII ACGT 3 cut(s) 26, 312, 943
MaeIII GTNAC 3 cut(s) 224, 247, 664
MalI GATC 2 cut(s) 140, 222
MbiI CCGCTC 1 cut(s) 13
MboI GATC 2 cut(s) 138, 220
MfeI CAATTG 1 cut(s) 1044
MhlI GDGCHC 1 cut(s) 213
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 8 cut(s) 363, 621, 774, 849, 927, 1044, 1152, 1215
MluNI TGGCCA 1 cut(s) 41
Mly113I GGCGCC 1 cut(s) 233
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 447
MscI TGGCCA 1 cut(s) 41
MslI CAYNNNNRTG 1 cut(s) 630
Msp20I TGGCCA 1 cut(s) 41
MspI CCGG 2 cut(s) 831, 1089
MunI CAATTG 1 cut(s) 1044
MwoI GCNNNNNNNGC 5 cut(s) 62, 73, 176, 251, 808
NarI GGCGCC 1 cut(s) 233
NdeII GATC 2 cut(s) 138, 220
NlaIII CATG 7 cut(s) 71, 170, 245, 424, 635, 654, 1019
NlaIV GGNNCC 2 cut(s) 234, 1007
NmuCI GTSAC 3 cut(s) 224, 247, 664
NspI RCATGY 3 cut(s) 71, 635, 654
PaeI GCATGC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 2 cut(s) 189, 796
PflFI GACNNNGTC 1 cut(s) 377
PkrI GCNGC 3 cut(s) 493, 743, 907
PluTI GGCGCC 1 cut(s) 236
Ppu21I YACGTR 1 cut(s) 944
PsiI TTATAA 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 616
PspN4I GGNNCC 2 cut(s) 234, 1007
PstI CTGCAG 1 cut(s) 750
PstNI CAGNNNCTG 2 cut(s) 799, 1103
PsyI GACNNNGTC 1 cut(s) 377
RsaI GTAC 4 cut(s) 933, 942, 1116, 1123
RsaNI GTAC 4 cut(s) 932, 941, 1115, 1122
RseI CAYNNNNRTG 1 cut(s) 630
SatI GCNGC 3 cut(s) 492, 742, 906
Sau3AI GATC 2 cut(s) 138, 220
SbfI CCTGCAGG 1 cut(s) 750
ScaI AGTACT 1 cut(s) 1123
SdaI CCTGCAGG 1 cut(s) 750
SduI GDGCHC 1 cut(s) 213
SfcI CTRYAG 1 cut(s) 746
SfoI GGCGCC 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 630
SnaBI TACGTA 1 cut(s) 944
SphI GCATGC 1 cut(s) 71
Sse8387I CCTGCAGG 1 cut(s) 750
Sse9I AATT 8 cut(s) 363, 621, 774, 849, 927, 1044, 1152, 1215
SsiI CCGC 3 cut(s) 13, 106, 124
SspDI GGCGCC 1 cut(s) 232
SspMI CTAG 6 cut(s) 359, 405, 539, 837, 1023, 1074
TaaI ACNGT 2 cut(s) 91, 1080
TaiI ACGT 3 cut(s) 29, 315, 946
TaqI TCGA 1 cut(s) 1060
TasI AATT 8 cut(s) 363, 621, 774, 849, 927, 1044, 1152, 1215
TatI WGTACW 3 cut(s) 931, 1114, 1121
TfiI GAWTC 2 cut(s) 189, 796
TscAI CASTG 4 cut(s) 137, 213, 233, 669
TseFI GTSAC 3 cut(s) 224, 247, 664
TseI GCWGC 3 cut(s) 491, 741, 905
Tsp45I GTSAC 3 cut(s) 224, 247, 664
TspDTI ATGAA 6 cut(s) 230, 645, 785, 1065, 1117, 1126
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 4 cut(s) 137, 213, 233, 669
Tth111I GACNNNGTC 1 cut(s) 377
XapI RAATTY 2 cut(s) 774, 1152
XceI RCATGY 3 cut(s) 71, 635, 654
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 6 cut(s) 359, 405, 539, 837, 1023, 1074
ZrmI AGTACT 1 cut(s) 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.