Rh4DG347400

Transmembrane Fragile-X-F protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004923
Physical Location & Seq
Reverse (-)
56904520 .. 56910293
5774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG347400.1

Sequence Viewer

Length: 1272 bp
ATGTTGGTAGAGCGGAGAGGGATGACGTGGCGCTCCGTGGCCAAGTCCCTGCAAGCTCTTGTGGCGCATGCCCTGCTTTCCACATTCACCGTTTTCCTCGTCCTCCGCCTCCACCACGCCGTCCGCTACCACTGGTGGATCATATTCTCCCCTCTGTGGCTTTTCCATGCAGTTGTAGCAAGAGGCAGATTCTCATTACCAGCTCCAGTGCCCCCTAATGATCGTCACTGGGCGCCACTTCATGCTGTCACGGCAACCCCCTTGCTTGTTGCGTTTGAACTACTGCTTTGTATATATTTAGAGAACAGATACGTTGTAAGCTTGAAGATTGTCTTTGTGCCATTGTTGGCATTTGAACTAGCAATTTTATTTGACAATGTCAGGATGTGTAAGGCTCTAATGCCTAGTGATGATGACACCATGAGTGATGATGTGATATGGGAAACTCTTCCTCACTTCTGGATTTCTATATCTATGGTCTTCCTTATTGCTGCCACAACATTCACTCTTCTAAAGATAAATGGTGATATTTCTGCTCTAGGCTGGTGGGACTTATTTATAAACTTTGGCATTGCACAGATGTTTGCCTTTCTTGTTTGCACGAAGTGGTATAACCCAGCAATTCACAGACATGCTCACTCTGGAGAAGCATGTTCATCTTCAGTGACCATAAGATATCTTGATTGGAATAGAGGCTTTGTAGTTTCTTCAGAAGAAGACCAAGAAGAGAGTGGAATATGCAGCCTGCAGGATATTGGTGGACATATTATGAAAATTCCTCTAATCACTTTCCAGATTCTGCTGTTTATGCGCTTAGAGGGAACACCATCCGGTGCTAGTCAAATCTCAATTCCACTTCTTTTTGCTCCTCTTCTTTTACTGCAAGGAGCTGGTGTTTTATATGCTGCATATAGGTTGATAGAGAAAATTGTACTTTTAGTACGTAGTGGAGTTGTTTCTGAAAGATACTTGGTCATAACATCAAAAGTTCGTGATTATTTTGGGTTCCTGCGTCATGGTTCTAGGCTTCTGGGTTGGTGGTCAATTGATGAAGGAAGTCGAGAGGAACAAGCTAGACTGTATTCTGCCGGAGCTTCAGGGTATAACACTTTCTCACCTGATGTAGTGAAGAAGATGCCTAGGTCGGACCTTGTTGAGGAGATTTGGAAACTGCAAACTGCACTTAGTGAGCAAACAGAAATCACGAAATTTAGCCAGCAGGAGTATGAAAGACTTCAGAATGTAATATCTCAGCTGACTTCTGGTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_185A zf-C3HC4_3

Protein Analysis

423

Amino Acids

48.08

Weight (kDa)

6.67

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_185A PF10269 35 - 294 7.4e-91 Transmembrane Fragile-X-F protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AccB1I GGYRCC 1 cut(s) 232
AccBSI CCGCTC 1 cut(s) 13
AciI CCGC 3 cut(s) 13, 106, 124
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 2 cut(s) 774, 1208
AcuI CTGAAG 4 cut(s) 645, 693, 1080, 1220
AcyI GRCGYC 1 cut(s) 233
AdeI CACNNNGTG 2 cut(s) 606, 1187
AfaI GTAC 2 cut(s) 933, 942
AfiI CCNNNNNNNGG 2 cut(s) 156, 1156
AgsI TTSAA 3 cut(s) 278, 325, 356
AhdI GACNNNNNGTC 1 cut(s) 1263
AjiI CACGTC 1 cut(s) 27
AluBI AGCT 7 cut(s) 56, 203, 321, 890, 1073, 1094, 1255
AluI AGCT 7 cut(s) 56, 203, 321, 890, 1073, 1094, 1255
AlwI GGATC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 799
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 3 cut(s) 491, 741, 905
ApoI RAATTY 2 cut(s) 774, 1208
Asp700I GAANNNNTTC 2 cut(s) 447, 1233
AspA2I CCTAGG 1 cut(s) 1139
AspLEI GCGC 4 cut(s) 33, 67, 235, 813
AspS9I GGNCC 1 cut(s) 1147
AsuHPI GGTGA 3 cut(s) 79, 536, 1107
AvaII GGWCC 1 cut(s) 1147
AvrII CCTAGG 1 cut(s) 1139
BaeGI GKGCMC 1 cut(s) 213
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 232
BbsI GAAGAC 2 cut(s) 472, 723
BbvI GCAGC 3 cut(s) 478, 753, 892
BccI CCATC 1 cut(s) 835
BceAI ACGGC 2 cut(s) 104, 267
BfaI CTAG 7 cut(s) 359, 405, 539, 837, 1023, 1074, 1140
BfmI CTRYAG 1 cut(s) 746
BfoI RGCGCY 2 cut(s) 34, 236
BisI GCNGC 3 cut(s) 492, 742, 906
BlnI CCTAGG 1 cut(s) 1139
BlsI GCNGC 3 cut(s) 493, 743, 907
Bme18I GGWCC 1 cut(s) 1147
BmeRI GACNNNNNGTC 1 cut(s) 1263
BmgBI CACGTC 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 1147
BmiI GGNNCC 2 cut(s) 234, 1007
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 1125
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 2 cut(s) 472, 723
BpmI CTGGAG 2 cut(s) 189, 663
BsaAI YACGTR 1 cut(s) 944
BsaHI GRCGYC 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 36, 1139
BsaWI WCCGGW 1 cut(s) 830
BsaXI ACNNNNNCTCC 2 cut(s) 879, 909
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 1156
Bse1I ACTGG 3 cut(s) 137, 206, 233
Bse3DI GCAATG 1 cut(s) 570
BseDI CCNNGG 2 cut(s) 36, 1139
BseGI GGATG 3 cut(s) 27, 390, 827
BseLI CCNNNNNNNGG 2 cut(s) 156, 1156
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 1 cut(s) 1265
BseNI ACTGG 3 cut(s) 137, 206, 233
BseRI GAGGAG 2 cut(s) 858, 1172
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 3 cut(s) 478, 753, 892
BseYI CCCAGC 1 cut(s) 616
BsgI GTGCAG 1 cut(s) 1164
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 1 cut(s) 232
BsiSI CCGG 2 cut(s) 831, 1089
BslFI GGGAC 2 cut(s) 31, 563
BslI CCNNNNNNNGG 2 cut(s) 156, 1156
BsmFI GGGAC 2 cut(s) 31, 563
BsnI GGCC 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 2 cut(s) 138, 220
BspACI CCGC 3 cut(s) 13, 106, 124
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 1 cut(s) 1264
BspLI GGNNCC 2 cut(s) 234, 1007
BspMAI CTGCAG 1 cut(s) 750
BspPI GGATC 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 232
BsrBI CCGCTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 3 cut(s) 137, 206, 233
BssECI CCNNGG 2 cut(s) 36, 1139
BssMI GATC 2 cut(s) 138, 220
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 1139
Bst4CI ACNGT 2 cut(s) 91, 1080
Bst6I CTCTTC 4 cut(s) 453, 513, 720, 876
BstACI GRCGYC 1 cut(s) 233
BstAPI GCANNNNNTGC 1 cut(s) 73
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 4 cut(s) 54, 69, 746, 1217
BstDEI CTNAG 3 cut(s) 814, 1184, 1251
BstDSI CCRYGG 1 cut(s) 36
BstENI CCTNNNNNAGG 1 cut(s) 1154
BstF5I GGATG 3 cut(s) 27, 390, 827
BstH2I RGCGCY 2 cut(s) 34, 236
BstHHI GCGC 4 cut(s) 33, 67, 235, 813
BstKTI GATC 2 cut(s) 141, 223
BstMBI GATC 2 cut(s) 138, 220
BstMWI GCNNNNNNNGC 5 cut(s) 62, 73, 176, 251, 808
BstNSI RCATGY 3 cut(s) 71, 635, 654
BstSFI CTRYAG 1 cut(s) 746
BstSLI GKGCMC 1 cut(s) 213
BstSNI TACGTA 1 cut(s) 944
BstV1I GCAGC 3 cut(s) 478, 753, 892
BstV2I GAAGAC 2 cut(s) 472, 723
BsuRI GGCC 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 36
BtrI CACGTC 1 cut(s) 27
BtsCI GGATG 3 cut(s) 27, 390, 827
BtsIMutI CAGTG 4 cut(s) 130, 213, 226, 669
Cac8I GCNNGC 4 cut(s) 54, 69, 746, 1217
CaiI CAGNNNCTG 1 cut(s) 799
CfoI GCGC 4 cut(s) 33, 67, 235, 813
Cfr13I GGNCC 1 cut(s) 1147
CseI GACGC 1 cut(s) 1001
Csp6I GTAC 2 cut(s) 932, 941
CviAII CATG 7 cut(s) 68, 167, 242, 421, 632, 651, 1016
CviQI GTAC 2 cut(s) 932, 941
DdeI CTNAG 3 cut(s) 814, 1184, 1251
DinI GGCGCC 1 cut(s) 234
DpnI GATC 2 cut(s) 140, 222
DpnII GATC 2 cut(s) 138, 220
DraIII CACNNNGTG 2 cut(s) 606, 1187
DriI GACNNNNNGTC 1 cut(s) 1263
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 4 cut(s) 453, 513, 720, 876
Eam1105I GACNNNNNGTC 1 cut(s) 1263
EarI CTCTTC 4 cut(s) 453, 513, 720, 876
EciI GGCGGA 1 cut(s) 95
Eco105I TACGTA 1 cut(s) 944
Eco130I CCWWGG 1 cut(s) 1139
Eco32I GATATC 1 cut(s) 677
Eco47I GGWCC 1 cut(s) 1147
Eco57I CTGAAG 4 cut(s) 645, 693, 1080, 1220
EcoNI CCTNNNNNAGG 1 cut(s) 1154
EcoRV GATATC 1 cut(s) 677
EcoT14I CCWWGG 1 cut(s) 1139
EgeI GGCGCC 1 cut(s) 234
EheI GGCGCC 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 1139
FaeI CATG 7 cut(s) 71, 170, 245, 424, 635, 654, 1019
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 2 cut(s) 31, 563
FatI CATG 7 cut(s) 67, 166, 241, 420, 631, 650, 1015
Fnu4HI GCNGC 3 cut(s) 492, 742, 906
FokI GGATG 3 cut(s) 34, 397, 814
Fsp4HI GCNGC 3 cut(s) 492, 742, 906
FspBI CTAG 7 cut(s) 359, 405, 539, 837, 1023, 1074, 1140
GlaI GCGC 4 cut(s) 32, 66, 234, 812
GluI GCNGC 3 cut(s) 492, 742, 906
GsaI CCCAGC 1 cut(s) 620
GsuI CTGGAG 2 cut(s) 189, 663
HaeII RGCGCY 2 cut(s) 34, 236
HaeIII GGCC 1 cut(s) 41
HapII CCGG 2 cut(s) 831, 1089
HgaI GACGC 1 cut(s) 1001
HhaI GCGC 4 cut(s) 33, 67, 235, 813
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 7 cut(s) 71, 170, 245, 424, 635, 654, 1019
Hin6I GCGC 4 cut(s) 31, 65, 233, 811
HinP1I GCGC 4 cut(s) 31, 65, 233, 811
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 2 cut(s) 189, 796
HpaII CCGG 2 cut(s) 831, 1089
HphI GGTGA 3 cut(s) 79, 536, 1107
Hpy166II GTNNAC 1 cut(s) 761
Hpy188I TCNGA 4 cut(s) 712, 961, 1147, 1239
Hpy188III TCNNGA 8 cut(s) 382, 460, 642, 680, 793, 992, 1061, 1204
Hpy8I GTNNAC 1 cut(s) 761
HpyAV CCTTC 1 cut(s) 1046
HpyCH4III ACNGT 2 cut(s) 91, 1080
HpyCH4IV ACGT 3 cut(s) 26, 312, 943
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 73, 176, 251, 808
HpyF3I CTNAG 3 cut(s) 814, 1184, 1251
HpySE526I ACGT 3 cut(s) 26, 312, 943
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 7 cut(s) 71, 170, 245, 424, 635, 654, 1019
HspAI GCGC 4 cut(s) 31, 65, 233, 811
KasI GGCGCC 1 cut(s) 232
Kzo9I GATC 2 cut(s) 138, 220
LmnI GCTCC 5 cut(s) 38, 208, 871, 887, 1091
Lsp1109I GCAGC 3 cut(s) 478, 753, 892
LweI GCATC 1 cut(s) 1125
MaeI CTAG 7 cut(s) 359, 405, 539, 837, 1023, 1074, 1140
MaeII ACGT 3 cut(s) 26, 312, 943
MaeIII GTNAC 3 cut(s) 224, 247, 664
MalI GATC 2 cut(s) 140, 222
MbiI CCGCTC 1 cut(s) 13
MboI GATC 2 cut(s) 138, 220
MfeI CAATTG 1 cut(s) 1044
MhlI GDGCHC 1 cut(s) 213
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 7 cut(s) 363, 621, 774, 849, 927, 1044, 1208
MluNI TGGCCA 1 cut(s) 41
Mly113I GGCGCC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 1125
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 2 cut(s) 447, 1233
MscI TGGCCA 1 cut(s) 41
MslI CAYNNNNRTG 1 cut(s) 630
Msp20I TGGCCA 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 1255
MspI CCGG 2 cut(s) 831, 1089
MunI CAATTG 1 cut(s) 1044
MwoI GCNNNNNNNGC 5 cut(s) 62, 73, 176, 251, 808
NarI GGCGCC 1 cut(s) 233
NdeII GATC 2 cut(s) 138, 220
NlaIII CATG 7 cut(s) 71, 170, 245, 424, 635, 654, 1019
NlaIV GGNNCC 2 cut(s) 234, 1007
NmuCI GTSAC 3 cut(s) 224, 247, 664
NspI RCATGY 3 cut(s) 71, 635, 654
PaeI GCATGC 1 cut(s) 71
PdmI GAANNNNTTC 2 cut(s) 447, 1233
PfeI GAWTC 2 cut(s) 189, 796
PflFI GACNNNGTC 1 cut(s) 377
PkrI GCNGC 3 cut(s) 493, 743, 907
PluTI GGCGCC 1 cut(s) 236
Ppu21I YACGTR 1 cut(s) 944
PsiI TTATAA 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 616
PspN4I GGNNCC 2 cut(s) 234, 1007
PspPI GGNCC 1 cut(s) 1147
PstI CTGCAG 1 cut(s) 750
PstNI CAGNNNCTG 1 cut(s) 799
PsyI GACNNNGTC 1 cut(s) 377
PvuII CAGCTG 1 cut(s) 1255
RsaI GTAC 2 cut(s) 933, 942
RsaNI GTAC 2 cut(s) 932, 941
RseI CAYNNNNRTG 1 cut(s) 630
SatI GCNGC 3 cut(s) 492, 742, 906
Sau3AI GATC 2 cut(s) 138, 220
Sau96I GGNCC 1 cut(s) 1147
SbfI CCTGCAGG 1 cut(s) 750
SdaI CCTGCAGG 1 cut(s) 750
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 1 cut(s) 1125
SfcI CTRYAG 1 cut(s) 746
SfoI GGCGCC 1 cut(s) 234
SinI GGWCC 1 cut(s) 1147
SmiMI CAYNNNNRTG 1 cut(s) 630
SnaBI TACGTA 1 cut(s) 944
SphI GCATGC 1 cut(s) 71
Sse8387I CCTGCAGG 1 cut(s) 750
Sse9I AATT 7 cut(s) 363, 621, 774, 849, 927, 1044, 1208
SsiI CCGC 3 cut(s) 13, 106, 124
SspDI GGCGCC 1 cut(s) 232
SspMI CTAG 7 cut(s) 359, 405, 539, 837, 1023, 1074, 1140
StyI CCWWGG 1 cut(s) 1139
TaaI ACNGT 2 cut(s) 91, 1080
TaiI ACGT 3 cut(s) 29, 315, 946
TaqI TCGA 1 cut(s) 1060
TasI AATT 7 cut(s) 363, 621, 774, 849, 927, 1044, 1208
TatI WGTACW 1 cut(s) 931
TfiI GAWTC 2 cut(s) 189, 796
TscAI CASTG 4 cut(s) 137, 213, 233, 669
TseFI GTSAC 3 cut(s) 224, 247, 664
TseI GCWGC 3 cut(s) 491, 741, 905
Tsp45I GTSAC 3 cut(s) 224, 247, 664
TspDTI ATGAA 5 cut(s) 230, 645, 785, 1065, 1242
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 4 cut(s) 137, 213, 233, 669
Tth111I GACNNNGTC 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 1147
XagI CCTNNNNNAGG 1 cut(s) 1154
XapI RAATTY 2 cut(s) 774, 1208
XceI RCATGY 3 cut(s) 71, 635, 654
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmaJI CCTAGG 1 cut(s) 1139
XmnI GAANNNNTTC 2 cut(s) 447, 1233
XspI CTAG 7 cut(s) 359, 405, 539, 837, 1023, 1074, 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.