Rroxscaffold_5G00377060

Transmembrane Fragile-X-F protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004923
Physical Location & Seq
Reverse (-)
57614707 .. 57619130
4424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00377060.1

Sequence Viewer

Length: 1347 bp
ATGTTGGTAGAGTGGAGAGGGATGACGTGGCGCTCCGTGGCCAAGTCCCTGCAAGCTCTTGTGGCGCATGCCCTGCTTTTAACATTCACCGTTTTCCTCGTCCTCCGCCTCCACCACCCCGTCCGCTACCACTGGTGGATCATATTCTCCCCTCTGTGGCTTTTCCATGCTGTTGTAGCAAGAGGCAGATTCTCATTACCAGCTCCAGTGCCACCTAATGATCGTCACTGGGCGCCACTTCATGCTGTCATGGCAACCCCCTTGCTCGTTGCGTTTGAACTACTGCTTTGTATATATCTAGAGAACAGATACGTTGTAAGCTTGAAGATTGTCTTTGTGCCATTGTTGGCGTTTGAACTAGCAATTTTATTTGACAATGTCAGGATGTGTAAGGCTCTAATGCCTAGTGATGATGACACCATGAGCGATGATGTGATATGGGAAACTCTTCCTCACTTCTGGATTTCTATATCTATGGTCTTCCTTATTGCTGCCACAACATTCACTCTTCTAAAGATAAATGGTGATATTTCTGCTCTAGGCTGGTGGGACTTATTTATAAACTTTGGCATTGCACAGATGTTTGCCTTTCTTGTTTGCACGAAGTGGTATAACCCAGCAATTCACAGACATGCTCACTCTGGAGAAGCATGTTCATCTTCAGTGACCATAAGATATCTTGATTGGAATAGAGGCTTCGTAGTTTCTTCAGAAGAAGACCAAGAAGAGAGTGGAATATGCAGCCTGCAGGATATTGGTGGACATATTATGAAAATTCCTCTAATCACTTTCCAGATTCTGCTGTTTATGCGCTTAGAGGGAACACCATCCGGTGCTAGTCAAATCTCAATTCCACTTCTTTTGCTCCTCTTCTTTTACTGCAAGGAGCTGGTGTTTTATATGCTGCATATAGGCTTCTGGGTTGGTGGTCAATTGATGAAGGAAGTCGAGAGGAACAAGCTAGACTGTATTCTGCCGGAGCTTCAGGGAAATACAAAATGTTTTATGCTTCGCTTAGTTCAATTGTTTGACTTCTTGCGTGTGGATATCTTGGTCTCTTATTGTGCATATGTGCTCAAGCATTTCAACAGATATGGGATGTTTCCATCTTGTATTTGTATGATGAATGTGGAAGTGGAATCATTTGAGTTGTTGCATAGGTATAACACTTTCTCACCTGATTTAGTGAAGAAGATGCCTAGGTCGGACCTTGTTGAGGAGATTTGGAAACTGCAAGCTGCACTTAGTGAGCAAACAGAAGTCACAAAATTTAGCCAGCAGGAGTATGAAAGACTTCAGAATGTAATATCTCAGCTGACTTCTGGTCTTTCAATGTTGGTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_185A zf-C3HC4_3

Protein Analysis

448

Amino Acids

51.75

Weight (kDa)

6.2

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_185A PF10269 35 - 289 2.7e-88 Transmembrane Fragile-X-F protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AccB1I GGYRCC 1 cut(s) 232
AciI CCGC 2 cut(s) 106, 124
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 2 cut(s) 774, 1268
AcuI CTGAAG 4 cut(s) 645, 693, 968, 1280
AcyI GRCGYC 1 cut(s) 233
AdeI CACNNNGTG 2 cut(s) 606, 1247
AfiI CCNNNNNNNGG 2 cut(s) 156, 1216
AgsI TTSAA 6 cut(s) 278, 325, 356, 1022, 1087, 1332
AhdI GACNNNNNGTC 1 cut(s) 1323
AjiI CACGTC 1 cut(s) 27
AluBI AGCT 8 cut(s) 56, 203, 321, 889, 961, 982, 1238, 1315
AluI AGCT 8 cut(s) 56, 203, 321, 889, 961, 982, 1238, 1315
Alw21I GWGCWC 1 cut(s) 1077
Alw26I GTCTC 1 cut(s) 1060
AlwI GGATC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 799
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 4 cut(s) 491, 741, 904, 1238
ApoI RAATTY 2 cut(s) 774, 1268
Asp700I GAANNNNTTC 2 cut(s) 447, 1293
AspA2I CCTAGG 1 cut(s) 1199
AspLEI GCGC 4 cut(s) 33, 67, 235, 813
AspS9I GGNCC 1 cut(s) 1207
AsuHPI GGTGA 3 cut(s) 79, 536, 1167
AvaII GGWCC 1 cut(s) 1207
AvrII CCTAGG 1 cut(s) 1199
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 232
BbsI GAAGAC 2 cut(s) 472, 723
Bbv12I GWGCWC 1 cut(s) 1077
BbvI GCAGC 4 cut(s) 478, 753, 891, 1225
BccI CCATC 2 cut(s) 835, 1114
BcoDI GTCTC 1 cut(s) 1060
BfaI CTAG 7 cut(s) 299, 359, 405, 539, 837, 962, 1200
BfmI CTRYAG 1 cut(s) 746
BfoI RGCGCY 2 cut(s) 34, 236
BisI GCNGC 4 cut(s) 492, 742, 905, 1239
BlnI CCTAGG 1 cut(s) 1199
BlsI GCNGC 4 cut(s) 493, 743, 906, 1240
Bme18I GGWCC 1 cut(s) 1207
BmeRI GACNNNNNGTC 1 cut(s) 1323
BmgBI CACGTC 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 1207
BmiI GGNNCC 1 cut(s) 234
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 1185
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 2 cut(s) 472, 723
BpmI CTGGAG 2 cut(s) 189, 663
BpuEI CTTGAG 1 cut(s) 1061
BsaHI GRCGYC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 1060
BsaJI CCNNGG 2 cut(s) 36, 1199
BsaWI WCCGGW 1 cut(s) 830
BsaXI ACNNNNNCTCC 2 cut(s) 878, 908
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 1216
Bse1I ACTGG 3 cut(s) 137, 206, 233
Bse3DI GCAATG 1 cut(s) 570
BseDI CCNNGG 2 cut(s) 36, 1199
BseGI GGATG 4 cut(s) 27, 390, 827, 1104
BseLI CCNNNNNNNGG 2 cut(s) 156, 1216
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 1 cut(s) 1325
BseNI ACTGG 3 cut(s) 137, 206, 233
BseRI GAGGAG 2 cut(s) 857, 1232
BseXI GCAGC 4 cut(s) 478, 753, 891, 1225
BseYI CCCAGC 1 cut(s) 616
BsgI GTGCAG 1 cut(s) 1224
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 1077
BsiSI CCGG 2 cut(s) 831, 977
BslFI GGGAC 2 cut(s) 31, 563
BslI CCNNNNNNNGG 2 cut(s) 156, 1216
BsmAI GTCTC 1 cut(s) 1060
BsmFI GGGAC 2 cut(s) 31, 563
BsnI GGCC 1 cut(s) 41
Bso31I GGTCTC 1 cut(s) 1060
Bsp1286I GDGCHC 1 cut(s) 1077
Bsp143I GATC 2 cut(s) 138, 220
BspACI CCGC 2 cut(s) 106, 124
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 1 cut(s) 1324
BspLI GGNNCC 1 cut(s) 234
BspMAI CTGCAG 1 cut(s) 750
BspPI GGATC 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 1060
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 3 cut(s) 137, 206, 233
BssECI CCNNGG 2 cut(s) 36, 1199
BssMI GATC 2 cut(s) 138, 220
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 1199
Bst4CI ACNGT 2 cut(s) 91, 968
Bst6I CTCTTC 4 cut(s) 453, 513, 720, 875
BstACI GRCGYC 1 cut(s) 233
BstAPI GCANNNNNTGC 1 cut(s) 73
BstC8I GCNNGC 5 cut(s) 54, 69, 746, 1236, 1277
BstDEI CTNAG 4 cut(s) 814, 1015, 1244, 1311
BstDSI CCRYGG 1 cut(s) 36
BstENI CCTNNNNNAGG 1 cut(s) 1214
BstF5I GGATG 4 cut(s) 27, 390, 827, 1104
BstH2I RGCGCY 2 cut(s) 34, 236
BstHHI GCGC 4 cut(s) 33, 67, 235, 813
BstKTI GATC 2 cut(s) 141, 223
BstMAI GTCTC 1 cut(s) 1060
BstMBI GATC 2 cut(s) 138, 220
BstMWI GCNNNNNNNGC 5 cut(s) 62, 73, 176, 251, 808
BstNSI RCATGY 3 cut(s) 71, 635, 654
BstSFI CTRYAG 1 cut(s) 746
BstV1I GCAGC 4 cut(s) 478, 753, 891, 1225
BstV2I GAAGAC 2 cut(s) 472, 723
BsuRI GGCC 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 36
BtgZI GCGATG 1 cut(s) 441
BtrI CACGTC 1 cut(s) 27
BtsCI GGATG 4 cut(s) 27, 390, 827, 1104
BtsIMutI CAGTG 4 cut(s) 130, 213, 226, 669
Cac8I GCNNGC 5 cut(s) 54, 69, 746, 1236, 1277
CaiI CAGNNNCTG 1 cut(s) 799
CfoI GCGC 4 cut(s) 33, 67, 235, 813
Cfr13I GGNCC 1 cut(s) 1207
CspCI CAANNNNNGTGG 2 cut(s) 843, 878
CviAII CATG 7 cut(s) 68, 167, 242, 250, 421, 632, 651
DdeI CTNAG 4 cut(s) 814, 1015, 1244, 1311
DinI GGCGCC 1 cut(s) 234
DpnI GATC 2 cut(s) 140, 222
DpnII GATC 2 cut(s) 138, 220
DraIII CACNNNGTG 2 cut(s) 606, 1247
DriI GACNNNNNGTC 1 cut(s) 1323
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 4 cut(s) 453, 513, 720, 875
Eam1105I GACNNNNNGTC 1 cut(s) 1323
EarI CTCTTC 4 cut(s) 453, 513, 720, 875
EciI GGCGGA 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 1199
Eco31I GGTCTC 1 cut(s) 1060
Eco32I GATATC 2 cut(s) 677, 1048
Eco47I GGWCC 1 cut(s) 1207
Eco57I CTGAAG 4 cut(s) 645, 693, 968, 1280
EcoNI CCTNNNNNAGG 1 cut(s) 1214
EcoRV GATATC 2 cut(s) 677, 1048
EcoT14I CCWWGG 1 cut(s) 1199
EgeI GGCGCC 1 cut(s) 234
EheI GGCGCC 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 1199
FaeI CATG 7 cut(s) 71, 170, 245, 253, 424, 635, 654
FalI AAGNNNNNCTT 4 cut(s) 317, 349, 1227, 1259
FaqI GGGAC 2 cut(s) 31, 563
FatI CATG 7 cut(s) 67, 166, 241, 249, 420, 631, 650
FauNDI CATATG 1 cut(s) 1069
Fnu4HI GCNGC 4 cut(s) 492, 742, 905, 1239
FokI GGATG 4 cut(s) 34, 397, 814, 1111
Fsp4HI GCNGC 4 cut(s) 492, 742, 905, 1239
FspBI CTAG 7 cut(s) 299, 359, 405, 539, 837, 962, 1200
GlaI GCGC 4 cut(s) 32, 66, 234, 812
GluI GCNGC 4 cut(s) 492, 742, 905, 1239
GsaI CCCAGC 1 cut(s) 620
GsuI CTGGAG 2 cut(s) 189, 663
HaeII RGCGCY 2 cut(s) 34, 236
HaeIII GGCC 1 cut(s) 41
HapII CCGG 2 cut(s) 831, 977
HhaI GCGC 4 cut(s) 33, 67, 235, 813
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 7 cut(s) 71, 170, 245, 253, 424, 635, 654
Hin6I GCGC 4 cut(s) 31, 65, 233, 811
HinP1I GCGC 4 cut(s) 31, 65, 233, 811
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 3 cut(s) 189, 796, 1139
HpaII CCGG 2 cut(s) 831, 977
HphI GGTGA 3 cut(s) 79, 536, 1167
Hpy166II GTNNAC 1 cut(s) 761
Hpy188I TCNGA 3 cut(s) 712, 1207, 1299
Hpy188III TCNNGA 7 cut(s) 299, 382, 460, 642, 680, 793, 949
Hpy8I GTNNAC 1 cut(s) 761
HpyAV CCTTC 1 cut(s) 934
HpyCH4III ACNGT 2 cut(s) 91, 968
HpyCH4IV ACGT 2 cut(s) 26, 312
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 73, 176, 251, 808
HpyF3I CTNAG 4 cut(s) 814, 1015, 1244, 1311
HpySE526I ACGT 2 cut(s) 26, 312
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 7 cut(s) 71, 170, 245, 253, 424, 635, 654
HspAI GCGC 4 cut(s) 31, 65, 233, 811
KasI GGCGCC 1 cut(s) 232
Kzo9I GATC 2 cut(s) 138, 220
LmnI GCTCC 5 cut(s) 38, 208, 870, 886, 979
Lsp1109I GCAGC 4 cut(s) 478, 753, 891, 1225
LweI GCATC 1 cut(s) 1185
MaeI CTAG 7 cut(s) 299, 359, 405, 539, 837, 962, 1200
MaeII ACGT 2 cut(s) 26, 312
MaeIII GTNAC 3 cut(s) 224, 664, 1261
MalI GATC 2 cut(s) 140, 222
MboI GATC 2 cut(s) 138, 220
MfeI CAATTG 2 cut(s) 932, 1022
MhlI GDGCHC 1 cut(s) 1077
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 7 cut(s) 363, 621, 774, 849, 932, 1022, 1268
MluNI TGGCCA 1 cut(s) 41
Mly113I GGCGCC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 1185
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 2 cut(s) 447, 1293
MscI TGGCCA 1 cut(s) 41
MseI TTAA 2 cut(s) 80, 1345
MslI CAYNNNNRTG 1 cut(s) 630
Msp20I TGGCCA 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 1315
MspI CCGG 2 cut(s) 831, 977
MunI CAATTG 2 cut(s) 932, 1022
MwoI GCNNNNNNNGC 5 cut(s) 62, 73, 176, 251, 808
NarI GGCGCC 1 cut(s) 233
NdeI CATATG 1 cut(s) 1069
NdeII GATC 2 cut(s) 138, 220
NlaIII CATG 7 cut(s) 71, 170, 245, 253, 424, 635, 654
NlaIV GGNNCC 1 cut(s) 234
NmuCI GTSAC 3 cut(s) 224, 664, 1261
NspI RCATGY 3 cut(s) 71, 635, 654
PaeI GCATGC 1 cut(s) 71
PdmI GAANNNNTTC 2 cut(s) 447, 1293
PfeI GAWTC 3 cut(s) 189, 796, 1139
PflFI GACNNNGTC 1 cut(s) 377
PkrI GCNGC 4 cut(s) 493, 743, 906, 1240
PluTI GGCGCC 1 cut(s) 236
PsiI TTATAA 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 616
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 1207
PstI CTGCAG 1 cut(s) 750
PstNI CAGNNNCTG 1 cut(s) 799
PsyI GACNNNGTC 1 cut(s) 377
PvuII CAGCTG 1 cut(s) 1315
RseI CAYNNNNRTG 1 cut(s) 630
SaqAI TTAA 2 cut(s) 80, 1345
SatI GCNGC 4 cut(s) 492, 742, 905, 1239
Sau3AI GATC 2 cut(s) 138, 220
Sau96I GGNCC 1 cut(s) 1207
SbfI CCTGCAGG 1 cut(s) 750
SdaI CCTGCAGG 1 cut(s) 750
SduI GDGCHC 1 cut(s) 1077
SfaNI GCATC 1 cut(s) 1185
SfcI CTRYAG 1 cut(s) 746
SfoI GGCGCC 1 cut(s) 234
SinI GGWCC 1 cut(s) 1207
SmiMI CAYNNNNRTG 1 cut(s) 630
SmlI CTYRAG 1 cut(s) 1076
SmoI CTYRAG 1 cut(s) 1076
SphI GCATGC 1 cut(s) 71
Sse8387I CCTGCAGG 1 cut(s) 750
Sse9I AATT 7 cut(s) 363, 621, 774, 849, 932, 1022, 1268
SsiI CCGC 2 cut(s) 106, 124
SspDI GGCGCC 1 cut(s) 232
SspMI CTAG 7 cut(s) 299, 359, 405, 539, 837, 962, 1200
StyI CCWWGG 1 cut(s) 1199
TaaI ACNGT 2 cut(s) 91, 968
TaiI ACGT 2 cut(s) 29, 315
TaqI TCGA 1 cut(s) 948
TasI AATT 7 cut(s) 363, 621, 774, 849, 932, 1022, 1268
TfiI GAWTC 3 cut(s) 189, 796, 1139
Tru1I TTAA 2 cut(s) 80, 1345
Tru9I TTAA 2 cut(s) 80, 1345
TscAI CASTG 4 cut(s) 137, 213, 233, 669
TseFI GTSAC 3 cut(s) 224, 664, 1261
TseI GCWGC 4 cut(s) 491, 741, 904, 1238
Tsp45I GTSAC 3 cut(s) 224, 664, 1261
TspDTI ATGAA 6 cut(s) 230, 645, 785, 953, 1139, 1302
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 4 cut(s) 137, 213, 233, 669
Tth111I GACNNNGTC 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 1207
XagI CCTNNNNNAGG 1 cut(s) 1214
XapI RAATTY 2 cut(s) 774, 1268
XbaI TCTAGA 1 cut(s) 298
XceI RCATGY 3 cut(s) 71, 635, 654
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmaJI CCTAGG 1 cut(s) 1199
XmnI GAANNNNTTC 2 cut(s) 447, 1293
XspI CTAG 7 cut(s) 299, 359, 405, 539, 837, 962, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.