Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004923

Physical Location & Seq

Genomic Coordinates Reverse (-)

3412774 .. 3418097

Gene Structure 5324 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1048600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1410 bp

ATGTTGGTTCAGAGGAGAGTGATGACGTGGCGGTCGGTGGCCAAGTCTCTCCAAACACTTGTCGCCCACGCGTCGCTTTTCACGTTCACGACTCTCCTCGTTCTCAAGCTCCACCGTTCCGTCCGCTACCCTTGGTGGATCATATTTTCCCCTCTCTGGCTGTTCCATGTAGTTGTAGCACGAGGCAGATTTTCGCTACCTGCTCCAAAGCTGCCTAATGATCGCAATTGGGCACCTTTTCATGCTGTCATGGCGACACCGTTGCTTGTCGCATTTGAACTAATGCTTTGTATATACCTTCAGAGCAGATATGTGTGTGCAGTTGTAAGCTTGAAGATTGTCTTTGCACCCTTGTTGGCATTTGAACTCGCAATTCTATTTGATAATGTCAGGATGTGTAAGGCTCTAATGCCTGGAGACGATGAACACGTAAACGATGATGTGATATGGGAAACGCTTCCTCACTTCTGGATTTCTATATCCATGGTCTTCTTTCTTGCTGCCACAGTATTCACTCTTTTAAAGATAAACGGTGATATTTCTGCTCTAGGCTGGTGGGACTTATTCATAAACTTTGGAATTGCACAGGGTTTTGCCTTTCTTGTTTGCACGAAGTGGTATAATCCAGCTATTCACAGACATGCTCACATTCAAGAACCCTGTTCATCTTCAATGAACGTAAGATACCTTGACTGGAATAGTGGCTTAGTCATTTCTTCAGATGAAGATCAAGACGAGACAGTAGTATGCAGCCTGCAGGATATCGGTGGGCATATTATGAAAGTTCCTCTAATCATTTTCCAGATCCTACTCTTTATGCACTTAGAGGGGAGGCCATCGGCTGCTAGGCATATCCCAATTCCAGTTCTTTTTGCTCCTCTTCTTTTACTGCAAGGAGTTGGTCTTTTGTTTTCCGCATATAGATTAACAGAGAAAATTGTTCTTTTAGTACATAATGGAGTTTTTTCTGTAAGATACGTGGACATAGCATCAAAAGTTCGCGAGTATTTTGGGTTCTATCGCCGTGGTTCAAGGTTATTGGGCTGGTGGTCGATTGACGAAGGAAGTCGAGAGGAACAGGCTAGACTGTATTGTGCAGGGGCTTCAGGGTACAACACTTTTTCACCCGATATAGTGAAGAAGTTGCCTAGATCGGACCTTGTTGAGGAGGTTTGGAAACTGCAAGCTGCACTCAGTGAGCAAACGGAAGTCACGAAATTTAGCCAGCAGGAGTACGAAAGACTTCAGAATGAAAAGATCCTATGTAGAGTATGCTTTGAAGAACAGATTAATATAGTCCTGCTTCCATGCCGGCATCACGTTCTTTGCAGCACTTGTTGTGAGAAGTGTAAAAAGTGCCCCATATGCCGTGTCTGCATTGAACAGCGACTGCCGATATATCATGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tmemb_185A zf-C3HC4_3

Protein Analysis

470

Amino Acids

53.94

Weight (kDa)

8.39

Isoelectric Point (pI)

45.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tmemb_185A	PF10269	35 - 297	8.6e-92	Transmembrane Fragile-X-F protein
zf-C3HC4_3	PF13920	420 - 462	8.8e-10	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004923)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68820 AT1G68820 AT1G68820 AT1G68820
fragaria_vesca	FvH4_4g28120 FvH4_4g28120 FvH4_4g28120 FvH4_4g28120 FvH4_4g28120
malus_domestica	MD13G1047000.v1.1 MD16G1048600.v1.1
prunus_persica	Prupe.1G305000_v2.0.a1 Prupe.1G305000_v2.0.a1 Prupe.1G305000_v2.0.a1 Prupe.1G305000_v2.0.a1
pyrus_communis	pycom13g04110 pycom16g04250
rosa_chinensis	RchiOBHm_Chr4g0435891
rosa_laevigata	RLG00000006552
rosa_multiflora	Rmu_sc0003165.1_g000009
rosa_roxburghii	Rroxscaffold_5G00377060
rosa_rugosa	Rorug04G0290300 Rorug04G0290400 Rorug04G0290500 Rorug04G0290600
rosa_samantha	Rh4AG344800 Rh4BG353600 Rh4CG368000 Rh4DG347400
rosa_wichuraiana	Rw4G030110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	31
Acc36I	ACCTGC	1 cut(s)	208
AccB1I	GGYRCC	1 cut(s)	232
AccII	CGCG	2 cut(s)	71, 1002
AciI	CCGC	3 cut(s)	31, 124, 915
AclWI	GGATC	3 cut(s)	146, 799, 1252
AcoI	YGGCCR	1 cut(s)	39
AcsI	RAATTY	1 cut(s)	1217
AcuI	CTGAAG	4 cut(s)	284, 702, 1089, 1229
AdeI	CACNNNGTG	2 cut(s)	615, 1196
AfaI	GTAC	3 cut(s)	951, 1112, 1235
AfiI	CCNNNNNNNGG	2 cut(s)	156, 1165
AflIII	ACRYGT	2 cut(s)	69, 427
AgsI	TTSAA	8 cut(s)	278, 334, 365, 653, 672, 1032, 1280, 1382
AjiI	CACGTC	1 cut(s)	27
AjnI	CCWGG	1 cut(s)	412
AluBI	AGCT	5 cut(s)	109, 211, 330, 629, 1187
AluI	AGCT	5 cut(s)	109, 211, 330, 629, 1187
Alw26I	GTCTC	3 cut(s)	51, 411, 731
AlwI	GGATC	3 cut(s)	146, 799, 1252
AlwNI	CAGNNNCTG	1 cut(s)	1390
AoxI	GGCC	2 cut(s)	39, 833
ApeKI	GCWGC	6 cut(s)	211, 500, 750, 842, 1187, 1329
ApoI	RAATTY	1 cut(s)	1217
AseI	ATTAAT	1 cut(s)	1290
Asp700I	GAANNNNTTC	2 cut(s)	456, 1242
AspS9I	GGNCC	1 cut(s)	1156
AsuHPI	GGTGA	2 cut(s)	545, 1116
AvaII	GGWCC	1 cut(s)	1156
BaeGI	GKGCMC	2 cut(s)	235, 1361
BalI	TGGCCA	1 cut(s)	41
BanI	GGYRCC	1 cut(s)	232
BauI	CACGAG	1 cut(s)	180
BbsI	GAAGAC	1 cut(s)	481
BbvI	GCAGC	6 cut(s)	198, 487, 762, 829, 1174, 1341
BccI	CCATC	1 cut(s)	844
BceAI	ACGGC	2 cut(s)	1008, 1353
BcgI	CGANNNNNNTGC	2 cut(s)	244, 278
BciT130I	CCWGG	1 cut(s)	414
BcoDI	GTCTC	3 cut(s)	51, 411, 731
BfaI	CTAG	4 cut(s)	548, 846, 1083, 1149
BfmI	CTRYAG	1 cut(s)	755
BfuAI	ACCTGC	1 cut(s)	208
BisI	GCNGC	6 cut(s)	212, 501, 751, 843, 1188, 1330
BlsI	GCNGC	6 cut(s)	213, 502, 752, 844, 1189, 1331
Bme1390I	CCNGG	1 cut(s)	414
Bme18I	GGWCC	1 cut(s)	1156
BmgBI	CACGTC	1 cut(s)	27
BmgT120I	GGNCC	1 cut(s)	1156
BmiI	GGNNCC	1 cut(s)	234
BmrFI	CCNGG	1 cut(s)	414
BmsI	GCATC	2 cut(s)	998, 1324
BpiI	GAAGAC	1 cut(s)	481
BpmI	CTGGAG	1 cut(s)	435
BpuEI	CTTGAG	1 cut(s)	89
BsaAI	YACGTR	2 cut(s)	430, 979
BsaBI	GATNNNNATC	1 cut(s)	726
BsaJI	CCNNGG	3 cut(s)	131, 483, 1024
Bsc4I	CCNNNNNNNGG	2 cut(s)	156, 1165
Bse118I	RCCGGY	1 cut(s)	1311
Bse1I	ACTGG	2 cut(s)	698, 863
Bse8I	GATNNNNATC	1 cut(s)	726
BseBI	CCWGG	1 cut(s)	414
BseDI	CCNNGG	3 cut(s)	131, 483, 1024
BseGI	GGATG	1 cut(s)	399
BseJI	GATNNNNATC	1 cut(s)	726
BseLI	CCNNNNNNNGG	2 cut(s)	156, 1165
BseMII	CTCAG	1 cut(s)	1207
BseNI	ACTGG	2 cut(s)	698, 863
BseRI	GAGGAG	4 cut(s)	28, 86, 867, 1181
BseSI	GKGCMC	2 cut(s)	235, 1361
BseXI	GCAGC	6 cut(s)	198, 487, 762, 829, 1174, 1341
BsgI	GTGCAG	3 cut(s)	339, 1116, 1173
Bsh1236I	CGCG	2 cut(s)	71, 1002
Bsh1285I	CGRYCG	1 cut(s)	35
BshFI	GGCC	2 cut(s)	41, 835
BshNI	GGYRCC	1 cut(s)	232
BsiEI	CGRYCG	1 cut(s)	35
BsiSI	CCGG	1 cut(s)	1312
BslFI	GGGAC	1 cut(s)	572
BslI	CCNNNNNNNGG	2 cut(s)	156, 1165
BsmAI	GTCTC	3 cut(s)	51, 411, 731
BsmBI	CGTCTC	1 cut(s)	411
BsmFI	GGGAC	1 cut(s)	572
BsnI	GGCC	2 cut(s)	41, 835
Bsp1286I	GDGCHC	2 cut(s)	235, 1361
Bsp143I	GATC	6 cut(s)	138, 220, 727, 804, 1151, 1257
Bsp19I	CCATGG	1 cut(s)	483
Bsp68I	TCGCGA	1 cut(s)	1002
BspACI	CCGC	3 cut(s)	31, 124, 915
BspANI	GGCC	2 cut(s)	41, 835
BspCNI	CTCAG	1 cut(s)	1206
BspFNI	CGCG	2 cut(s)	71, 1002
BspLI	GGNNCC	1 cut(s)	234
BspMAI	CTGCAG	1 cut(s)	759
BspMI	ACCTGC	1 cut(s)	208
BspPI	GGATC	3 cut(s)	146, 799, 1252
BspT107I	GGYRCC	1 cut(s)	232
BsrFI	RCCGGY	1 cut(s)	1311
BsrI	ACTGG	2 cut(s)	698, 863
BssAI	RCCGGY	1 cut(s)	1311
BssECI	CCNNGG	3 cut(s)	131, 483, 1024
BssMI	GATC	6 cut(s)	138, 220, 727, 804, 1151, 1257
BssSI	CACGAG	1 cut(s)	180
BssT1I	CCWWGG	2 cut(s)	131, 483
Bst2BI	CACGAG	1 cut(s)	180
Bst2UI	CCWGG	1 cut(s)	414
Bst4CI	ACNGT	6 cut(s)	116, 261, 508, 533, 742, 1089
Bst6I	CTCTTC	1 cut(s)	885
BstBAI	YACGTR	2 cut(s)	430, 979
BstC8I	GCNNGC	4 cut(s)	755, 1185, 1226, 1313
BstDEI	CTNAG	3 cut(s)	706, 823, 1193
BstDSI	CCRYGG	2 cut(s)	483, 1024
BstENI	CCTNNNNNAGG	1 cut(s)	1163
BstF5I	GGATG	1 cut(s)	399
BstFNI	CGCG	2 cut(s)	71, 1002
BstKTI	GATC	6 cut(s)	141, 223, 730, 807, 1154, 1260
BstMAI	GTCTC	3 cut(s)	51, 411, 731
BstMBI	GATC	6 cut(s)	138, 220, 727, 804, 1151, 1257
BstMCI	CGRYCG	1 cut(s)	35
BstMWI	GCNNNNNNNGC	3 cut(s)	251, 1365, 1374
BstNI	CCWGG	1 cut(s)	414
BstNSI	RCATGY	1 cut(s)	644
BstSCI	CCNGG	1 cut(s)	412
BstSFI	CTRYAG	1 cut(s)	755
BstSLI	GKGCMC	2 cut(s)	235, 1361
BstUI	CGCG	2 cut(s)	71, 1002
BstV1I	GCAGC	6 cut(s)	198, 487, 762, 829, 1174, 1341
BstV2I	GAAGAC	1 cut(s)	481
BstX2I	RGATCY	2 cut(s)	804, 1257
BstYI	RGATCY	2 cut(s)	804, 1257
BsuRI	GGCC	2 cut(s)	41, 835
BtgI	CCRYGG	2 cut(s)	483, 1024
BtrI	CACGTC	1 cut(s)	27
BtsCI	GGATG	1 cut(s)	399
BtsIMutI	CAGTG	1 cut(s)	1201
BtuMI	TCGCGA	1 cut(s)	1002
BveI	ACCTGC	1 cut(s)	208
Cac8I	GCNNGC	4 cut(s)	755, 1185, 1226, 1313
CaiI	CAGNNNCTG	1 cut(s)	1390
Cfr10I	RCCGGY	1 cut(s)	1311
Cfr13I	GGNCC	1 cut(s)	1156
CseI	GACGC	1 cut(s)	60
Csp6I	GTAC	3 cut(s)	950, 1111, 1234
CviAII	CATG	7 cut(s)	167, 242, 250, 484, 641, 1308, 1403
CviQI	GTAC	3 cut(s)	950, 1111, 1234
DdeI	CTNAG	3 cut(s)	706, 823, 1193
DpnI	GATC	6 cut(s)	140, 222, 729, 806, 1153, 1259
DpnII	GATC	6 cut(s)	138, 220, 727, 804, 1151, 1257
DraI	TTTAAA	1 cut(s)	522
DraIII	CACNNNGTG	2 cut(s)	615, 1196
DrdI	GACNNNNNNGTC	1 cut(s)	31
DseDI	GACNNNNNNGTC	1 cut(s)	31
EaeI	YGGCCR	1 cut(s)	39
Eam1104I	CTCTTC	1 cut(s)	885
EarI	CTCTTC	1 cut(s)	885
Eco130I	CCWWGG	2 cut(s)	131, 483
Eco32I	GATATC	1 cut(s)	763
Eco47I	GGWCC	1 cut(s)	1156
Eco57I	CTGAAG	4 cut(s)	284, 702, 1089, 1229
EcoNI	CCTNNNNNAGG	1 cut(s)	1163
EcoRII	CCWGG	1 cut(s)	412
EcoRV	GATATC	1 cut(s)	763
EcoT14I	CCWWGG	2 cut(s)	131, 483
ErhI	CCWWGG	2 cut(s)	131, 483
Esp3I	CGTCTC	1 cut(s)	411
FaeI	CATG	7 cut(s)	170, 245, 253, 487, 644, 1311, 1406
FalI	AAGNNNNNCTT	2 cut(s)	326, 358
FaqI	GGGAC	1 cut(s)	572
FatI	CATG	7 cut(s)	166, 241, 249, 483, 640, 1307, 1402
FauNDI	CATATG	1 cut(s)	1364
Fnu4HI	GCNGC	6 cut(s)	212, 501, 751, 843, 1188, 1330
FokI	GGATG	1 cut(s)	406
Fsp4HI	GCNGC	6 cut(s)	212, 501, 751, 843, 1188, 1330
FspBI	CTAG	4 cut(s)	548, 846, 1083, 1149
GluI	GCNGC	6 cut(s)	212, 501, 751, 843, 1188, 1330
GsuI	CTGGAG	1 cut(s)	435
HaeIII	GGCC	2 cut(s)	41, 835
HapII	CCGG	1 cut(s)	1312
HgaI	GACGC	1 cut(s)	60
Hin1II	CATG	7 cut(s)	170, 245, 253, 487, 644, 1311, 1406
HindIII	AAGCTT	1 cut(s)	328
HinfI	GANTC	1 cut(s)	91
HpaII	CCGG	1 cut(s)	1312
HphI	GGTGA	2 cut(s)	545, 1116
Hpy166II	GTNNAC	3 cut(s)	87, 433, 982
Hpy188I	TCNGA	5 cut(s)	12, 303, 721, 1156, 1248
Hpy188III	TCNNGA	9 cut(s)	88, 391, 469, 653, 731, 802, 1001, 1070, 1213
Hpy8I	GTNNAC	3 cut(s)	87, 433, 982
Hpy99I	CGWCG	1 cut(s)	76
HpyAV	CCTTC	2 cut(s)	308, 1055
HpyCH4III	ACNGT	6 cut(s)	116, 261, 508, 533, 742, 1089
HpyCH4IV	ACGT	6 cut(s)	26, 83, 429, 678, 978, 1320
HpyF10VI	GCNNNNNNNGC	3 cut(s)	251, 1365, 1374
HpyF3I	CTNAG	3 cut(s)	706, 823, 1193
HpySE526I	ACGT	6 cut(s)	26, 83, 429, 678, 978, 1320
Hsp92II	CATG	7 cut(s)	170, 245, 253, 487, 644, 1311, 1406
KroI	GCCGGC	1 cut(s)	1311
KroNI	GCCGGC	1 cut(s)	1313
Kzo9I	GATC	6 cut(s)	138, 220, 727, 804, 1151, 1257
LmnI	GCTCC	3 cut(s)	114, 208, 880
Lsp1109I	GCAGC	6 cut(s)	198, 487, 762, 829, 1174, 1341
LweI	GCATC	2 cut(s)	998, 1324
MaeI	CTAG	4 cut(s)	548, 846, 1083, 1149
MaeII	ACGT	6 cut(s)	26, 83, 429, 678, 978, 1320
MaeIII	GTNAC	1 cut(s)	1210
MalI	GATC	6 cut(s)	140, 222, 729, 806, 1153, 1259
MboI	GATC	6 cut(s)	138, 220, 727, 804, 1151, 1257
MboII	GAAGA	8 cut(s)	346, 481, 660, 708, 737, 872, 1150, 1292
MfeI	CAATTG	1 cut(s)	226
MflI	RGATCY	2 cut(s)	804, 1257
MhlI	GDGCHC	2 cut(s)	235, 1361
MlsI	TGGCCA	1 cut(s)	41
MluCI	AATT	6 cut(s)	226, 372, 579, 858, 936, 1217
MluI	ACGCGT	1 cut(s)	69
MluNI	TGGCCA	1 cut(s)	41
MlyI	GAGTC	1 cut(s)	85
Mox20I	TGGCCA	1 cut(s)	41
MroNI	GCCGGC	1 cut(s)	1311
MroXI	GAANNNNTTC	2 cut(s)	456, 1242
MscI	TGGCCA	1 cut(s)	41
MseI	TTAA	3 cut(s)	521, 926, 1290
MslI	CAYNNNNRTG	1 cut(s)	639
Msp20I	TGGCCA	1 cut(s)	41
MspI	CCGG	1 cut(s)	1312
MspR9I	CCNGG	1 cut(s)	414
MunI	CAATTG	1 cut(s)	226
MvaI	CCWGG	1 cut(s)	414
MvnI	CGCG	2 cut(s)	71, 1002
MwoI	GCNNNNNNNGC	3 cut(s)	251, 1365, 1374
NaeI	GCCGGC	1 cut(s)	1313
NcoI	CCATGG	1 cut(s)	483
NdeI	CATATG	1 cut(s)	1364
NdeII	GATC	6 cut(s)	138, 220, 727, 804, 1151, 1257
NgoMIV	GCCGGC	1 cut(s)	1311
NlaIII	CATG	7 cut(s)	170, 245, 253, 487, 644, 1311, 1406
NlaIV	GGNNCC	1 cut(s)	234
NmuCI	GTSAC	1 cut(s)	1210
NruI	TCGCGA	1 cut(s)	1002
NspI	RCATGY	1 cut(s)	644
PcsI	WCGNNNNNNNCGW	3 cut(s)	80, 426, 1211
PdiI	GCCGGC	1 cut(s)	1313
PdmI	GAANNNNTTC	2 cut(s)	456, 1242
PkrI	GCNGC	6 cut(s)	213, 502, 752, 844, 1189, 1331
PleI	GAGTC	1 cut(s)	85
PpsI	GAGTC	1 cut(s)	85
Ppu21I	YACGTR	2 cut(s)	430, 979
PshBI	ATTAAT	1 cut(s)	1290
Psp6I	CCWGG	1 cut(s)	412
PspGI	CCWGG	1 cut(s)	412
PspN4I	GGNNCC	1 cut(s)	234
PspPI	GGNCC	1 cut(s)	1156
PsrI	GAACNNNNNNTAC	4 cut(s)	668, 700, 998, 1030
PstI	CTGCAG	1 cut(s)	759
PstNI	CAGNNNCTG	1 cut(s)	1390
PsuI	RGATCY	2 cut(s)	804, 1257
RruI	TCGCGA	1 cut(s)	1002
RsaI	GTAC	3 cut(s)	951, 1112, 1235
RsaNI	GTAC	3 cut(s)	950, 1111, 1234
RseI	CAYNNNNRTG	1 cut(s)	639
SaqAI	TTAA	3 cut(s)	521, 926, 1290
SatI	GCNGC	6 cut(s)	212, 501, 751, 843, 1188, 1330
Sau3AI	GATC	6 cut(s)	138, 220, 727, 804, 1151, 1257
Sau96I	GGNCC	1 cut(s)	1156
SbfI	CCTGCAGG	1 cut(s)	759
SchI	GAGTC	1 cut(s)	85
ScrFI	CCNGG	1 cut(s)	414
SdaI	CCTGCAGG	1 cut(s)	759
SduI	GDGCHC	2 cut(s)	235, 1361
SfaNI	GCATC	2 cut(s)	998, 1324
SfcI	CTRYAG	1 cut(s)	755
SinI	GGWCC	1 cut(s)	1156
SmiMI	CAYNNNNRTG	1 cut(s)	639
SmlI	CTYRAG	1 cut(s)	104
SmoI	CTYRAG	1 cut(s)	104
Sse8387I	CCTGCAGG	1 cut(s)	759
Sse9I	AATT	6 cut(s)	226, 372, 579, 858, 936, 1217
SsiI	CCGC	3 cut(s)	31, 124, 915
SspMI	CTAG	4 cut(s)	548, 846, 1083, 1149
StyD4I	CCNGG	1 cut(s)	412
StyI	CCWWGG	2 cut(s)	131, 483
TaaI	ACNGT	6 cut(s)	116, 261, 508, 533, 742, 1089
TaiI	ACGT	6 cut(s)	29, 86, 432, 681, 981, 1323
TaqI	TCGA	2 cut(s)	1052, 1069
TasI	AATT	6 cut(s)	226, 372, 579, 858, 936, 1217
TatI	WGTACW	1 cut(s)	949
Tru1I	TTAA	3 cut(s)	521, 926, 1290
Tru9I	TTAA	3 cut(s)	521, 926, 1290
TscAI	CASTG	1 cut(s)	1201
TseFI	GTSAC	1 cut(s)	1210
TseI	GCWGC	6 cut(s)	211, 500, 750, 842, 1187, 1329
Tsp45I	GTSAC	1 cut(s)	1210
TspDTI	ATGAA	8 cut(s)	230, 438, 556, 654, 689, 738, 794, 1266
TspGWI	ACGGA	2 cut(s)	109, 1220
TspRI	CASTG	1 cut(s)	1201
VpaK11BI	GGWCC	1 cut(s)	1156
VspI	ATTAAT	1 cut(s)	1290
XagI	CCTNNNNNAGG	1 cut(s)	1163
XapI	RAATTY	1 cut(s)	1217
XceI	RCATGY	1 cut(s)	644
XmnI	GAANNNNTTC	2 cut(s)	456, 1242
XspI	CTAG	4 cut(s)	548, 846, 1083, 1149

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.