FvH4_4g28120

Transmembrane Fragile-X-F protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004923
Physical Location & Seq
Reverse (-)
28855717 .. 28861867
6151 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g28120.t3

Sequence Viewer

Length: 1401 bp
ATGCTGGTGCAACGGAGAGTGATGACGTGGCGCTCCGTGGCCAAGTCTCTGCAAGCTCTTTCGGCGCACGCCTTGCTTTTCACATTCACCGTCTTCCTCGCTCTCCGGCTCCACCACGCCGTCCGCTACCCCTGGTGGATTGTATTTTCCCCTCTATGGCTTTTCCATGCAGTTGTAGCACGAGGCAGATTCTCATTACCGGCTCCAGTTCCGCCTAATGGTCGCCACTGGGCACCACTTCATGCTGTCATGGCGACCCCGTTACTCGTTGCGTTTGAACTACTGCTTTGTATGTATCTACAGAACAGATACGCTCTAAGCTTGAAGATTGTCTTTGTGCCATTGTTGGCATTTGAACTAGCAATTCTATTTGACAATGTCAGGATGTGTAAGGCCCTAATGCCTAGTGATGAAGACACCTTGAATGATGATGTGATATGGGAAACACTTCCTCACTTCTGGATTTCTATATCCATGGTCTTCCTTATTGCAGCCACAACATTCACTCTTCTAAAGATAAATGGTGATATTTCTGCTCTGGGCTGGTGGGACTTATTTATAAACTTCGGTATCGCACAGATGTTTGCCTTTCTTGTTTGCACGAAGTGGTATAATCCAGCAATTCACAGACATGCTCACTCTGGAGAAGCATGTTCATCTTCAATGACCATAAGATATCTAGATTGGAATAGAGGGTTTGTAGTATCTTCAGACGACGACCAAGAAGAGAGTGGAATATGTAGCCTCCAGGATATTGGTGGACATTTTATGAAAATTCCTCTAATCACTTTCCAGATTCTACTGTTTATGCGTTTAGAGGGAACAACATCCGGTACTAGTCAAATATCAATTCCGCTTCTTTTTGCTCCTCTTCTTTTACTGCAAGGAGCTGGTGTTTTATATGCTGCATATAGGTTGATAGAGAAAATTGTACTTTTAGTACGTAGTGGAGCTGTTTCTGAAAGATACTTGGTCATAACATCAAAAGTTCGTGATTATTTTGGGTTCCTGCGTCATGGTTCTAGGCTTCTGGGTTGGTGGTCAATTGATGAGGGAAGTCGAGAGGAACAGGCTAGACTGTTTTCTGCTGGATCATCAGGGTATAATACTTTTTCACCTGATGTAGTGAAGAAGATGCCCAGGTTGGACCTTGTTGAGGAGATTTGGAAACTGCAAGCTGCACTTAGTGAGCAAACAGAAATCACAAAATTTAGCCAGCAGGAGTATGAAAGACTTCAGAATGAAAAGATCCTATGCAGAGTATGCTTTGAAGAACAGATTAATATAGTCCTCCTCCCATGCAGGCATCATGTTCTTTGCAGCTCTTGCTGTGAGAAGTGTAAAAAGTGCCCCATATGCCGGGTCTGTATCGAACAACGACTCCCGATTTATCATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_185A zf-C3HC4_3

Protein Analysis

467

Amino Acids

53.34

Weight (kDa)

8.31

Isoelectric Point (pI)

46.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_185A PF10269 35 - 294 4.7e-90 Transmembrane Fragile-X-F protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AccB1I GGYRCC 1 cut(s) 232
AciI CCGC 3 cut(s) 124, 212, 854
AclWI GGATC 2 cut(s) 1099, 1243
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 2 cut(s) 774, 1208
AcuI CTGAAG 2 cut(s) 693, 1220
AdeI CACNNNGTG 2 cut(s) 606, 1187
AfaI GTAC 3 cut(s) 835, 933, 942
AfiI CCNNNNNNNGG 4 cut(s) 156, 218, 1156, 1359
AgsI TTSAA 6 cut(s) 278, 325, 356, 424, 663, 1271
AhlI ACTAGT 1 cut(s) 836
AjiI CACGTC 1 cut(s) 27
AjnI CCWGG 3 cut(s) 131, 747, 1139
AloI GAACNNNNNNTCC 2 cut(s) 1365, 1397
AluBI AGCT 6 cut(s) 56, 321, 890, 953, 1178, 1323
AluI AGCT 6 cut(s) 56, 321, 890, 953, 1178, 1323
Alw26I GTCTC 1 cut(s) 51
AlwI GGATC 2 cut(s) 1099, 1243
AoxI GGCC 2 cut(s) 39, 393
ApeKI GCWGC 4 cut(s) 491, 905, 1178, 1320
ApoI RAATTY 2 cut(s) 774, 1208
AseI ATTAAT 1 cut(s) 1281
Asp700I GAANNNNTTC 2 cut(s) 447, 1233
AspLEI GCGC 2 cut(s) 33, 67
AspS9I GGNCC 2 cut(s) 394, 1147
AsuC2I CCSGG 1 cut(s) 1361
AsuHPI GGTGA 3 cut(s) 79, 536, 1107
AvaII GGWCC 1 cut(s) 1147
BaeGI GKGCMC 2 cut(s) 235, 1352
BaeI ACNNNNGTAYC 2 cut(s) 553, 586
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 232
BauI CACGAG 1 cut(s) 180
BbsI GAAGAC 3 cut(s) 85, 420, 472
BbvI GCAGC 4 cut(s) 503, 892, 1165, 1332
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 3 cut(s) 133, 749, 1141
BcnI CCSGG 1 cut(s) 1361
BcoDI GTCTC 1 cut(s) 51
BcuI ACTAGT 1 cut(s) 836
BfaI CTAG 6 cut(s) 359, 405, 680, 837, 1023, 1074
BfmI CTRYAG 1 cut(s) 299
BfoI RGCGCY 1 cut(s) 34
BisI GCNGC 4 cut(s) 492, 906, 1179, 1321
BlsI GCNGC 4 cut(s) 493, 907, 1180, 1322
Bme1390I CCNGG 4 cut(s) 133, 749, 1141, 1361
Bme18I GGWCC 1 cut(s) 1147
BmgBI CACGTC 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 394, 1147
BmiI GGNNCC 4 cut(s) 110, 204, 234, 1007
BmrFI CCNGG 4 cut(s) 133, 749, 1141, 1361
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 2 cut(s) 1125, 1315
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 3 cut(s) 85, 420, 472
BpmI CTGGAG 3 cut(s) 189, 663, 731
BpuMI CCSGG 1 cut(s) 1361
BsaAI YACGTR 1 cut(s) 944
BsaJI CCNNGG 4 cut(s) 36, 131, 474, 1139
BsaWI WCCGGW 1 cut(s) 830
BsaXI ACNNNNNCTCC 4 cut(s) 879, 909, 1365, 1395
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 218, 1156, 1359
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 2 cut(s) 206, 233
BseBI CCWGG 3 cut(s) 133, 749, 1141
BseDI CCNNGG 4 cut(s) 36, 131, 474, 1139
BseGI GGATG 2 cut(s) 390, 827
BseLI CCNNNNNNNGG 4 cut(s) 156, 218, 1156, 1359
BseNI ACTGG 2 cut(s) 206, 233
BseRI GAGGAG 3 cut(s) 858, 1172, 1283
BseSI GKGCMC 2 cut(s) 235, 1352
BseXI GCAGC 4 cut(s) 503, 892, 1165, 1332
BsgI GTGCAG 1 cut(s) 1164
BshFI GGCC 2 cut(s) 41, 395
BshNI GGYRCC 1 cut(s) 232
BsiSI CCGG 4 cut(s) 106, 200, 831, 1360
BslFI GGGAC 1 cut(s) 563
BslI CCNNNNNNNGG 4 cut(s) 156, 218, 1156, 1359
BsmAI GTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 563
BsnI GGCC 2 cut(s) 41, 395
Bsp1286I GDGCHC 2 cut(s) 235, 1352
Bsp143I GATC 2 cut(s) 1091, 1248
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 3 cut(s) 124, 212, 854
BspANI GGCC 2 cut(s) 41, 395
BspLI GGNNCC 4 cut(s) 110, 204, 234, 1007
BspPI GGATC 2 cut(s) 1099, 1243
BspT107I GGYRCC 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 2 cut(s) 206, 233
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 4 cut(s) 36, 131, 474, 1139
BssMI GATC 2 cut(s) 1091, 1248
BssSI CACGAG 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 180
Bst2UI CCWGG 3 cut(s) 133, 749, 1141
Bst4CI ACNGT 3 cut(s) 91, 804, 1080
Bst6I CTCTTC 3 cut(s) 513, 720, 876
BstAPI GCANNNNNTGC 3 cut(s) 73, 1263, 1326
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 5 cut(s) 54, 69, 1176, 1217, 1304
BstDEI CTNAG 2 cut(s) 317, 1184
BstDSI CCRYGG 2 cut(s) 36, 474
BstENI CCTNNNNNAGG 1 cut(s) 1154
BstF5I GGATG 2 cut(s) 390, 827
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 2 cut(s) 33, 67
BstKTI GATC 2 cut(s) 1094, 1251
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 2 cut(s) 1091, 1248
BstMWI GCNNNNNNNGC 7 cut(s) 62, 73, 176, 251, 1263, 1326, 1356
BstNI CCWGG 3 cut(s) 133, 749, 1141
BstNSI RCATGY 2 cut(s) 635, 654
BstSCI CCNGG 4 cut(s) 131, 747, 1139, 1359
BstSFI CTRYAG 1 cut(s) 299
BstSLI GKGCMC 2 cut(s) 235, 1352
BstSNI TACGTA 1 cut(s) 944
BstV1I GCAGC 4 cut(s) 503, 892, 1165, 1332
BstV2I GAAGAC 3 cut(s) 85, 420, 472
BstX2I RGATCY 1 cut(s) 1248
BstXI CCANNNNNNTGG 1 cut(s) 755
BstYI RGATCY 1 cut(s) 1248
BsuRI GGCC 2 cut(s) 41, 395
BtgI CCRYGG 2 cut(s) 36, 474
BtrI CACGTC 1 cut(s) 27
BtsCI GGATG 2 cut(s) 390, 827
BtsIMutI CAGTG 1 cut(s) 226
Cac8I GCNNGC 5 cut(s) 54, 69, 1176, 1217, 1304
CfoI GCGC 2 cut(s) 33, 67
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 394, 1147
CseI GACGC 1 cut(s) 1001
Csp6I GTAC 3 cut(s) 834, 932, 941
CviQI GTAC 3 cut(s) 834, 932, 941
DdeI CTNAG 2 cut(s) 317, 1184
DpnI GATC 2 cut(s) 1093, 1250
DpnII GATC 2 cut(s) 1091, 1248
DraIII CACNNNGTG 2 cut(s) 606, 1187
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 3 cut(s) 513, 720, 876
EarI CTCTTC 3 cut(s) 513, 720, 876
EciI GGCGGA 1 cut(s) 201
Eco105I TACGTA 1 cut(s) 944
Eco130I CCWWGG 1 cut(s) 474
Eco32I GATATC 1 cut(s) 677
Eco47I GGWCC 1 cut(s) 1147
Eco57I CTGAAG 2 cut(s) 693, 1220
EcoNI CCTNNNNNAGG 1 cut(s) 1154
EcoO109I RGGNCCY 1 cut(s) 394
EcoRII CCWGG 3 cut(s) 131, 747, 1139
EcoRV GATATC 1 cut(s) 677
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
FalI AAGNNNNNCTT 4 cut(s) 317, 349, 1167, 1199
FaqI GGGAC 1 cut(s) 563
FauNDI CATATG 1 cut(s) 1355
Fnu4HI GCNGC 4 cut(s) 492, 906, 1179, 1321
FokI GGATG 2 cut(s) 397, 814
Fsp4HI GCNGC 4 cut(s) 492, 906, 1179, 1321
FspBI CTAG 6 cut(s) 359, 405, 680, 837, 1023, 1074
GlaI GCGC 2 cut(s) 32, 66
GluI GCNGC 4 cut(s) 492, 906, 1179, 1321
GsuI CTGGAG 3 cut(s) 189, 663, 731
HaeII RGCGCY 1 cut(s) 34
HaeIII GGCC 2 cut(s) 41, 395
HapII CCGG 4 cut(s) 106, 200, 831, 1360
HgaI GACGC 1 cut(s) 1001
HhaI GCGC 2 cut(s) 33, 67
Hin6I GCGC 2 cut(s) 31, 65
HinP1I GCGC 2 cut(s) 31, 65
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 3 cut(s) 189, 796, 1380
HpaII CCGG 4 cut(s) 106, 200, 831, 1360
HphI GGTGA 3 cut(s) 79, 536, 1107
Hpy166II GTNNAC 1 cut(s) 761
Hpy188I TCNGA 3 cut(s) 712, 961, 1239
Hpy188III TCNNGA 8 cut(s) 382, 460, 642, 680, 793, 992, 1061, 1384
Hpy8I GTNNAC 1 cut(s) 761
Hpy99I CGWCG 1 cut(s) 719
HpyCH4III ACNGT 3 cut(s) 91, 804, 1080
HpyCH4IV ACGT 2 cut(s) 26, 943
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 73, 176, 251, 1263, 1326, 1356
HpyF3I CTNAG 2 cut(s) 317, 1184
HpySE526I ACGT 2 cut(s) 26, 943
HspAI GCGC 2 cut(s) 31, 65
Kzo9I GATC 2 cut(s) 1091, 1248
LmnI GCTCC 6 cut(s) 38, 114, 208, 871, 887, 950
Lsp1109I GCAGC 4 cut(s) 503, 892, 1165, 1332
LweI GCATC 2 cut(s) 1125, 1315
MaeI CTAG 6 cut(s) 359, 405, 680, 837, 1023, 1074
MaeII ACGT 2 cut(s) 26, 943
MaeIII GTNAC 1 cut(s) 261
MalI GATC 2 cut(s) 1093, 1250
MboI GATC 2 cut(s) 1091, 1248
MfeI CAATTG 1 cut(s) 1044
MflI RGATCY 1 cut(s) 1248
MhlI GDGCHC 2 cut(s) 235, 1352
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 7 cut(s) 363, 621, 774, 849, 927, 1044, 1208
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 1 cut(s) 1374
MmeI TCCRAC 1 cut(s) 1125
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 2 cut(s) 447, 1233
MscI TGGCCA 1 cut(s) 41
MseI TTAA 1 cut(s) 1281
MslI CAYNNNNRTG 1 cut(s) 630
Msp20I TGGCCA 1 cut(s) 41
MspI CCGG 4 cut(s) 106, 200, 831, 1360
MspR9I CCNGG 4 cut(s) 133, 749, 1141, 1361
MunI CAATTG 1 cut(s) 1044
MvaI CCWGG 3 cut(s) 133, 749, 1141
MwoI GCNNNNNNNGC 7 cut(s) 62, 73, 176, 251, 1263, 1326, 1356
NciI CCSGG 1 cut(s) 1361
NcoI CCATGG 1 cut(s) 474
NdeI CATATG 1 cut(s) 1355
NdeII GATC 2 cut(s) 1091, 1248
NlaIV GGNNCC 4 cut(s) 110, 204, 234, 1007
NspI RCATGY 2 cut(s) 635, 654
PdmI GAANNNNTTC 2 cut(s) 447, 1233
PfeI GAWTC 2 cut(s) 189, 796
PflFI GACNNNGTC 1 cut(s) 377
PfoI TCCNGGA 1 cut(s) 747
PkrI GCNGC 4 cut(s) 493, 907, 1180, 1322
PleI GAGTC 1 cut(s) 1374
PpsI GAGTC 1 cut(s) 1374
Ppu21I YACGTR 1 cut(s) 944
PshBI ATTAAT 1 cut(s) 1281
PsiI TTATAA 1 cut(s) 560
Psp6I CCWGG 3 cut(s) 131, 747, 1139
PspGI CCWGG 3 cut(s) 131, 747, 1139
PspN4I GGNNCC 4 cut(s) 110, 204, 234, 1007
PspPI GGNCC 2 cut(s) 394, 1147
PsuI RGATCY 1 cut(s) 1248
PsyI GACNNNGTC 1 cut(s) 377
RsaI GTAC 3 cut(s) 835, 933, 942
RsaNI GTAC 3 cut(s) 834, 932, 941
RseI CAYNNNNRTG 1 cut(s) 630
SaqAI TTAA 1 cut(s) 1281
SatI GCNGC 4 cut(s) 492, 906, 1179, 1321
Sau3AI GATC 2 cut(s) 1091, 1248
Sau96I GGNCC 2 cut(s) 394, 1147
SchI GAGTC 1 cut(s) 1374
ScrFI CCNGG 4 cut(s) 133, 749, 1141, 1361
SduI GDGCHC 2 cut(s) 235, 1352
SfaNI GCATC 2 cut(s) 1125, 1315
SfcI CTRYAG 1 cut(s) 299
SinI GGWCC 1 cut(s) 1147
SmiMI CAYNNNNRTG 1 cut(s) 630
SnaBI TACGTA 1 cut(s) 944
SpeI ACTAGT 1 cut(s) 836
Sse9I AATT 7 cut(s) 363, 621, 774, 849, 927, 1044, 1208
SsiI CCGC 3 cut(s) 124, 212, 854
SspMI CTAG 6 cut(s) 359, 405, 680, 837, 1023, 1074
StyD4I CCNGG 4 cut(s) 131, 747, 1139, 1359
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 3 cut(s) 91, 804, 1080
TaiI ACGT 2 cut(s) 29, 946
TaqI TCGA 2 cut(s) 1060, 1371
TasI AATT 7 cut(s) 363, 621, 774, 849, 927, 1044, 1208
TatI WGTACW 1 cut(s) 931
TfiI GAWTC 2 cut(s) 189, 796
Tru1I TTAA 1 cut(s) 1281
Tru9I TTAA 1 cut(s) 1281
TscAI CASTG 1 cut(s) 233
TseI GCWGC 4 cut(s) 491, 905, 1178, 1320
TspDTI ATGAA 6 cut(s) 230, 426, 645, 785, 1242, 1257
TspGWI ACGGA 2 cut(s) 25, 28
TspRI CASTG 1 cut(s) 233
Tth111I GACNNNGTC 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 1147
VspI ATTAAT 1 cut(s) 1281
XagI CCTNNNNNAGG 1 cut(s) 1154
XapI RAATTY 2 cut(s) 774, 1208
XbaI TCTAGA 1 cut(s) 679
XceI RCATGY 2 cut(s) 635, 654
XcmI CCANNNNNNNNNTGG 2 cut(s) 728, 755
XmnI GAANNNNTTC 2 cut(s) 447, 1233
XspI CTAG 6 cut(s) 359, 405, 680, 837, 1023, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.