RchiOBHm_Chr4g0435891

Transmembrane Fragile-X-F protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004923
Physical Location & Seq
Reverse (-)
59298339 .. 59304102
5764 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40428

Sequence Viewer

Length: 1401 bp
ATGTTGGTAGAGCGGAGAGGGATGACGTGGCGCTCCGTGGCCAAGTCCCTGCAAGCTCTTGTGGCCCATGCCCTGCTTTTCACATTCACCGTTTTCCTCGTCCTCCGCCTCCACCACGCCGTCCGCTACCACTGGTGGATCATATTCTCCCCTCTGTGGCTTTTCCATGCAGTTGTAGCAAGAGGCAGATTCTCATTACCAGCTCCAGTGCCACCTAATGATCGTCACTGGGCGCCACTTCATGCTGTCACGGCAACCCCCTTGCTCGTTGCGTTTGAACTACTGCTTTGTATATATCTAGAGAACAGATACGTTGTAAGCTTGAAGATTGTCTTTGTGCCATTGTTGGCATTTGAACTAGCAATTTTATTTGACAATGTCAGGATGTGTAAGGCTCTAATGCCTAGTGATGATGACACCATGAGTGATGATGTGATATGGGAAACTCTTCCTCACTTCTGGATTTCTATATCTATGGTCTTCCTTATTGCTGCCACAACATTCACTCTTCTAAAGATAAATGGTGATATTTCTGCTCTAGGCTGGTGGGACTTATTTATAAACTTTGGCATTGCACAGATGTTTGCCTTTCTTGTTTGCACGAAGTGGTATAACCCAGCAATTCACAGACATGCTCACTCTGGAGAAGCATGTTCATCTTCAGTGACCATAAGATATCTTGATTGGAATAGAGGCTTTGTAGTTTCTTCAGAAGAAGACCAAGAAGAGAGTGGAATATGCAGCCTGCAGGATATTGGTGGACATATTATGAAAATTCCTCTAATCACTTTCCAGATTCTGCTGTTTATGCGCTTAGAGGGAACACCATCCGGTGCTAGTCAAATCTCAATTCCACTTCTTTTTGCTCCTCTTCTTTTACTGCAAGGAGCTGGTGTTTTATATGCTGCATATAGGTTGATAGAGAAAATTGTACTTTTAGTACGTAGTGGAGTTGTTTCTGAAAGATACTTGGTCATAACATCAAAAGTTCGTGATTATTTTGGGTTCCTGCGTCATGGTTCTAGGCTTCTGGGTTGGTGGTCAATTGATGAAGGAAGTCGAGAGGAACAAGCTAGACTGTATTCTGCCGGAGCTTCAGGGTATAACACTTTCTCACCTGATGTAGTGAAGAAGATGCCTAGGTCGGACCTTGTTGAGGAGATTTGGAAACTGCAAGCTGCACTTAGTGAGCAAACAGAAATCACGAAATTTAGCCAGCAGGAGTATGAAAGACTTCAGAATGAAAAGATCCTATGCAGAGTATGCTTTGAAGAACAGATTAACATAGTCCTCCTTCCATGCAGGCATCATGTGCTTTGCAGCTCTTGCTGTGAGAAGTGTAAAAAGTGCCCCATATGTCGGGTCTGTATTGAACAGAGACTCCCGATATATCATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_185A zf-C3HC4_3

Protein Analysis

466

Amino Acids

53.32

Weight (kDa)

7.57

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_185A PF10269 35 - 294 9.7e-91 Transmembrane Fragile-X-F protein
zf-C3HC4_3 PF13920 417 - 459 7.2e-10 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AccB1I GGYRCC 1 cut(s) 232
AccBSI CCGCTC 1 cut(s) 13
AciI CCGC 3 cut(s) 13, 106, 124
AclWI GGATC 2 cut(s) 146, 1243
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 2 cut(s) 774, 1208
AcuI CTGAAG 4 cut(s) 645, 693, 1080, 1220
AcyI GRCGYC 1 cut(s) 233
AdeI CACNNNGTG 2 cut(s) 606, 1187
AfaI GTAC 2 cut(s) 933, 942
AfiI CCNNNNNNNGG 3 cut(s) 156, 1156, 1359
AgsI TTSAA 5 cut(s) 278, 325, 356, 1271, 1373
AjiI CACGTC 1 cut(s) 27
AloI GAACNNNNNNTCC 2 cut(s) 1365, 1397
AluBI AGCT 8 cut(s) 56, 203, 321, 890, 1073, 1094, 1178, 1323
AluI AGCT 8 cut(s) 56, 203, 321, 890, 1073, 1094, 1178, 1323
Alw26I GTCTC 1 cut(s) 1372
AlwI GGATC 2 cut(s) 146, 1243
AlwNI CAGNNNCTG 1 cut(s) 799
AoxI GGCC 2 cut(s) 39, 63
ApeKI GCWGC 5 cut(s) 491, 741, 905, 1178, 1320
ApoI RAATTY 2 cut(s) 774, 1208
Asp700I GAANNNNTTC 2 cut(s) 447, 1233
AspA2I CCTAGG 1 cut(s) 1139
AspLEI GCGC 3 cut(s) 33, 235, 813
AspS9I GGNCC 2 cut(s) 64, 1147
AsuHPI GGTGA 3 cut(s) 79, 536, 1107
AvaII GGWCC 1 cut(s) 1147
AvrII CCTAGG 1 cut(s) 1139
BaeGI GKGCMC 1 cut(s) 1352
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 232
BbsI GAAGAC 2 cut(s) 472, 723
BbvI GCAGC 5 cut(s) 478, 753, 892, 1165, 1332
BccI CCATC 1 cut(s) 835
BceAI ACGGC 2 cut(s) 104, 267
BcoDI GTCTC 1 cut(s) 1372
BfaI CTAG 8 cut(s) 299, 359, 405, 539, 837, 1023, 1074, 1140
BfmI CTRYAG 1 cut(s) 746
BfoI RGCGCY 2 cut(s) 34, 236
BisI GCNGC 5 cut(s) 492, 742, 906, 1179, 1321
BlnI CCTAGG 1 cut(s) 1139
BlsI GCNGC 5 cut(s) 493, 743, 907, 1180, 1322
Bme18I GGWCC 1 cut(s) 1147
BmgBI CACGTC 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 64, 1147
BmiI GGNNCC 2 cut(s) 234, 1007
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 2 cut(s) 1125, 1315
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 2 cut(s) 472, 723
BpmI CTGGAG 2 cut(s) 189, 663
BsaAI YACGTR 1 cut(s) 944
BsaHI GRCGYC 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 36, 1139
BsaWI WCCGGW 1 cut(s) 830
BsaXI ACNNNNNCTCC 4 cut(s) 879, 909, 1365, 1395
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 1156, 1359
Bse1I ACTGG 3 cut(s) 137, 206, 233
Bse3DI GCAATG 1 cut(s) 570
BseDI CCNNGG 2 cut(s) 36, 1139
BseGI GGATG 3 cut(s) 27, 390, 827
BseLI CCNNNNNNNGG 3 cut(s) 156, 1156, 1359
BseMI GCAATG 1 cut(s) 570
BseNI ACTGG 3 cut(s) 137, 206, 233
BseRI GAGGAG 2 cut(s) 858, 1172
BseSI GKGCMC 1 cut(s) 1352
BseXI GCAGC 5 cut(s) 478, 753, 892, 1165, 1332
BseYI CCCAGC 1 cut(s) 616
BsgI GTGCAG 1 cut(s) 1164
BshFI GGCC 2 cut(s) 41, 65
BshNI GGYRCC 1 cut(s) 232
BsiSI CCGG 2 cut(s) 831, 1089
BslFI GGGAC 2 cut(s) 31, 563
BslI CCNNNNNNNGG 3 cut(s) 156, 1156, 1359
BsmAI GTCTC 1 cut(s) 1372
BsmFI GGGAC 2 cut(s) 31, 563
BsnI GGCC 2 cut(s) 41, 65
Bsp1286I GDGCHC 1 cut(s) 1352
Bsp143I GATC 3 cut(s) 138, 220, 1248
BspACI CCGC 3 cut(s) 13, 106, 124
BspANI GGCC 2 cut(s) 41, 65
BspLI GGNNCC 2 cut(s) 234, 1007
BspMAI CTGCAG 1 cut(s) 750
BspPI GGATC 2 cut(s) 146, 1243
BspT107I GGYRCC 1 cut(s) 232
BsrBI CCGCTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 3 cut(s) 137, 206, 233
BssECI CCNNGG 2 cut(s) 36, 1139
BssMI GATC 3 cut(s) 138, 220, 1248
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 1139
Bst4CI ACNGT 2 cut(s) 91, 1080
Bst6I CTCTTC 4 cut(s) 453, 513, 720, 876
BstACI GRCGYC 1 cut(s) 233
BstAPI GCANNNNNTGC 3 cut(s) 1263, 1312, 1326
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 5 cut(s) 54, 746, 1176, 1217, 1304
BstDEI CTNAG 2 cut(s) 814, 1184
BstDSI CCRYGG 1 cut(s) 36
BstENI CCTNNNNNAGG 1 cut(s) 1154
BstF5I GGATG 3 cut(s) 27, 390, 827
BstH2I RGCGCY 2 cut(s) 34, 236
BstHHI GCGC 3 cut(s) 33, 235, 813
BstKTI GATC 3 cut(s) 141, 223, 1251
BstMAI GTCTC 1 cut(s) 1372
BstMBI GATC 3 cut(s) 138, 220, 1248
BstMWI GCNNNNNNNGC 7 cut(s) 62, 176, 251, 808, 1263, 1312, 1326
BstNSI RCATGY 2 cut(s) 635, 654
BstSFI CTRYAG 1 cut(s) 746
BstSLI GKGCMC 1 cut(s) 1352
BstSNI TACGTA 1 cut(s) 944
BstV1I GCAGC 5 cut(s) 478, 753, 892, 1165, 1332
BstV2I GAAGAC 2 cut(s) 472, 723
BstX2I RGATCY 1 cut(s) 1248
BstYI RGATCY 1 cut(s) 1248
BsuRI GGCC 2 cut(s) 41, 65
BtgI CCRYGG 1 cut(s) 36
BtrI CACGTC 1 cut(s) 27
BtsCI GGATG 3 cut(s) 27, 390, 827
BtsIMutI CAGTG 4 cut(s) 130, 213, 226, 669
Cac8I GCNNGC 5 cut(s) 54, 746, 1176, 1217, 1304
CaiI CAGNNNCTG 1 cut(s) 799
CfoI GCGC 3 cut(s) 33, 235, 813
Cfr13I GGNCC 2 cut(s) 64, 1147
CseI GACGC 1 cut(s) 1001
Csp6I GTAC 2 cut(s) 932, 941
CviQI GTAC 2 cut(s) 932, 941
DdeI CTNAG 2 cut(s) 814, 1184
DinI GGCGCC 1 cut(s) 234
DpnI GATC 3 cut(s) 140, 222, 1250
DpnII GATC 3 cut(s) 138, 220, 1248
DraIII CACNNNGTG 2 cut(s) 606, 1187
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 4 cut(s) 453, 513, 720, 876
EarI CTCTTC 4 cut(s) 453, 513, 720, 876
EciI GGCGGA 1 cut(s) 95
Eco105I TACGTA 1 cut(s) 944
Eco130I CCWWGG 1 cut(s) 1139
Eco32I GATATC 1 cut(s) 677
Eco47I GGWCC 1 cut(s) 1147
Eco57I CTGAAG 4 cut(s) 645, 693, 1080, 1220
EcoNI CCTNNNNNAGG 1 cut(s) 1154
EcoRV GATATC 1 cut(s) 677
EcoT14I CCWWGG 1 cut(s) 1139
EgeI GGCGCC 1 cut(s) 234
EheI GGCGCC 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 1139
FalI AAGNNNNNCTT 4 cut(s) 317, 349, 1167, 1199
FaqI GGGAC 2 cut(s) 31, 563
FauNDI CATATG 1 cut(s) 1355
Fnu4HI GCNGC 5 cut(s) 492, 742, 906, 1179, 1321
FokI GGATG 3 cut(s) 34, 397, 814
Fsp4HI GCNGC 5 cut(s) 492, 742, 906, 1179, 1321
FspBI CTAG 8 cut(s) 299, 359, 405, 539, 837, 1023, 1074, 1140
GlaI GCGC 3 cut(s) 32, 234, 812
GluI GCNGC 5 cut(s) 492, 742, 906, 1179, 1321
GsaI CCCAGC 1 cut(s) 620
GsuI CTGGAG 2 cut(s) 189, 663
HaeII RGCGCY 2 cut(s) 34, 236
HaeIII GGCC 2 cut(s) 41, 65
HapII CCGG 2 cut(s) 831, 1089
HgaI GACGC 1 cut(s) 1001
HhaI GCGC 3 cut(s) 33, 235, 813
Hin1I GRCGYC 1 cut(s) 233
Hin6I GCGC 3 cut(s) 31, 233, 811
HinP1I GCGC 3 cut(s) 31, 233, 811
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 3 cut(s) 189, 796, 1380
HpaII CCGG 2 cut(s) 831, 1089
HphI GGTGA 3 cut(s) 79, 536, 1107
Hpy166II GTNNAC 1 cut(s) 761
Hpy188I TCNGA 4 cut(s) 712, 961, 1147, 1239
Hpy8I GTNNAC 1 cut(s) 761
HpyAV CCTTC 2 cut(s) 1046, 1304
HpyCH4III ACNGT 2 cut(s) 91, 1080
HpyCH4IV ACGT 3 cut(s) 26, 312, 943
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 176, 251, 808, 1263, 1312, 1326
HpyF3I CTNAG 2 cut(s) 814, 1184
HpySE526I ACGT 3 cut(s) 26, 312, 943
Hsp92I GRCGYC 1 cut(s) 233
HspAI GCGC 3 cut(s) 31, 233, 811
KasI GGCGCC 1 cut(s) 232
Kzo9I GATC 3 cut(s) 138, 220, 1248
LmnI GCTCC 5 cut(s) 38, 208, 871, 887, 1091
Lsp1109I GCAGC 5 cut(s) 478, 753, 892, 1165, 1332
LweI GCATC 2 cut(s) 1125, 1315
MaeI CTAG 8 cut(s) 299, 359, 405, 539, 837, 1023, 1074, 1140
MaeII ACGT 3 cut(s) 26, 312, 943
MaeIII GTNAC 3 cut(s) 224, 247, 664
MalI GATC 3 cut(s) 140, 222, 1250
MbiI CCGCTC 1 cut(s) 13
MboI GATC 3 cut(s) 138, 220, 1248
MfeI CAATTG 1 cut(s) 1044
MflI RGATCY 1 cut(s) 1248
MhlI GDGCHC 1 cut(s) 1352
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 7 cut(s) 363, 621, 774, 849, 927, 1044, 1208
MluNI TGGCCA 1 cut(s) 41
Mly113I GGCGCC 1 cut(s) 233
MlyI GAGTC 1 cut(s) 1374
MmeI TCCRAC 1 cut(s) 1125
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 2 cut(s) 447, 1233
MscI TGGCCA 1 cut(s) 41
MseI TTAA 1 cut(s) 1281
MslI CAYNNNNRTG 1 cut(s) 630
Msp20I TGGCCA 1 cut(s) 41
MspI CCGG 2 cut(s) 831, 1089
MunI CAATTG 1 cut(s) 1044
MwoI GCNNNNNNNGC 7 cut(s) 62, 176, 251, 808, 1263, 1312, 1326
NarI GGCGCC 1 cut(s) 233
NdeI CATATG 1 cut(s) 1355
NdeII GATC 3 cut(s) 138, 220, 1248
NlaIV GGNNCC 2 cut(s) 234, 1007
NmuCI GTSAC 3 cut(s) 224, 247, 664
NspI RCATGY 2 cut(s) 635, 654
PdmI GAANNNNTTC 2 cut(s) 447, 1233
PfeI GAWTC 2 cut(s) 189, 796
PflFI GACNNNGTC 1 cut(s) 377
PkrI GCNGC 5 cut(s) 493, 743, 907, 1180, 1322
PleI GAGTC 1 cut(s) 1374
PluTI GGCGCC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 1374
Ppu21I YACGTR 1 cut(s) 944
PsiI TTATAA 1 cut(s) 560
PspFI CCCAGC 1 cut(s) 616
PspN4I GGNNCC 2 cut(s) 234, 1007
PspPI GGNCC 2 cut(s) 64, 1147
PstI CTGCAG 1 cut(s) 750
PstNI CAGNNNCTG 1 cut(s) 799
PsuI RGATCY 1 cut(s) 1248
PsyI GACNNNGTC 1 cut(s) 377
RsaI GTAC 2 cut(s) 933, 942
RsaNI GTAC 2 cut(s) 932, 941
RseI CAYNNNNRTG 1 cut(s) 630
SaqAI TTAA 1 cut(s) 1281
SatI GCNGC 5 cut(s) 492, 742, 906, 1179, 1321
Sau3AI GATC 3 cut(s) 138, 220, 1248
Sau96I GGNCC 2 cut(s) 64, 1147
SbfI CCTGCAGG 1 cut(s) 750
SchI GAGTC 1 cut(s) 1374
SdaI CCTGCAGG 1 cut(s) 750
SduI GDGCHC 1 cut(s) 1352
SfaNI GCATC 2 cut(s) 1125, 1315
SfcI CTRYAG 1 cut(s) 746
SfoI GGCGCC 1 cut(s) 234
SinI GGWCC 1 cut(s) 1147
SmiMI CAYNNNNRTG 1 cut(s) 630
SnaBI TACGTA 1 cut(s) 944
Sse8387I CCTGCAGG 1 cut(s) 750
Sse9I AATT 7 cut(s) 363, 621, 774, 849, 927, 1044, 1208
SsiI CCGC 3 cut(s) 13, 106, 124
SspDI GGCGCC 1 cut(s) 232
SspMI CTAG 8 cut(s) 299, 359, 405, 539, 837, 1023, 1074, 1140
StyI CCWWGG 1 cut(s) 1139
TaaI ACNGT 2 cut(s) 91, 1080
TaiI ACGT 3 cut(s) 29, 315, 946
TaqI TCGA 1 cut(s) 1060
TasI AATT 7 cut(s) 363, 621, 774, 849, 927, 1044, 1208
TatI WGTACW 1 cut(s) 931
TfiI GAWTC 2 cut(s) 189, 796
Tru1I TTAA 1 cut(s) 1281
Tru9I TTAA 1 cut(s) 1281
TscAI CASTG 4 cut(s) 137, 213, 233, 669
TseFI GTSAC 3 cut(s) 224, 247, 664
TseI GCWGC 5 cut(s) 491, 741, 905, 1178, 1320
Tsp45I GTSAC 3 cut(s) 224, 247, 664
TspDTI ATGAA 6 cut(s) 230, 645, 785, 1065, 1242, 1257
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 4 cut(s) 137, 213, 233, 669
Tth111I GACNNNGTC 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 1147
XagI CCTNNNNNAGG 1 cut(s) 1154
XapI RAATTY 2 cut(s) 774, 1208
XbaI TCTAGA 1 cut(s) 298
XceI RCATGY 2 cut(s) 635, 654
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmaJI CCTAGG 1 cut(s) 1139
XmnI GAANNNNTTC 2 cut(s) 447, 1233
XspI CTAG 8 cut(s) 299, 359, 405, 539, 837, 1023, 1074, 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.