AT1G74480

RWP-RK domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012997
Physical Location & Seq
Reverse (-)
27993032 .. 27994178
1147 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74480.1

Sequence Viewer

Length: 897 bp
ATGGCTGATCACACAACCAAAGAACAGAAGTCATTCTCATTCCTAGCTCATTCTCCATCCTTTGATCACAGCTCCTTAAGTTATCCTTTATTCGACTGGGAAGAAGATCTTCTTGCTCTCCAAGAAAACTCTGGCTCTCAAGCATTTCCTTTTACTACAACTTCTCTGCCTTTACCTGATCTTGAACCCTTGTCTGAAGATGTACTCAATTCATACAGCTCTGCGTCATGGAACGAAACAGAGCAAAACAGAGGAGATGGCGCTTCATCGGAGAAGAAGAGGGAAAATGGAACAGTGAAAGAGACAACTAAGAAGAGGAAAATCAATGAGAGACACAGAGAACATAGCGTGAGAATCATCAGCGATATTACTACCTACACAACTAGTTCAGCTCCAACGACATTGTCAAAGGAAACTGTCTCTCGCTACTTCTACATGCCCATAACTCAGGCTGCAATAGCACTTAACGTTGGTTTAACTCTACTAAAAAGGAGATGTCGCGAATTGGGTATTCGCCGATGGCCTCATCGTAAACTTATGAGCTTAAACACTTTGATCAGTAACGTCAAGGAGCTGCAGAAGATGGAAGGCGAAGAGAATGCAGAAAAACTGCAGGACGCGTTGGAGATGCTTGAGAAGGAGAAGAGGACAATTGAGGATTTGCCGGATTTGGAGTTTAAGGACAAGACAAAGAGGCTAAGACAAGCTTGTTTCAAGGCTAACCACAAGAGGAAGAAGAAGAGAAGTCTCAAGTCCGATCAGTCTCAAGTACCCTCGTGTTCAAGCAGCGGATCAGTTCCTAGTGATGAGTCGGTTGATGAAGCAGGAATGGAGAGTGATGAAGAAATGAAGTATCTCTTGTGTGGTTTCTCAAGTGAATTTACTAGTGGTTTGTGA

Protein Analysis

298

Amino Acids

33.89

Weight (kDa)

6.23

Isoelectric Point (pI)

63.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 135 - 182 1.7e-21 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 501, 620
AciI CCGC 1 cut(s) 789
AclI AACGTT 1 cut(s) 468
AclWI GGATC 1 cut(s) 799
AcsI RAATTY 1 cut(s) 878
AcuI CTGAAG 1 cut(s) 216
AfaI GTAC 2 cut(s) 204, 771
AflII CTTAAG 1 cut(s) 76
AflIII ACRYGT 1 cut(s) 618
AgsI TTSAA 3 cut(s) 185, 715, 783
AhlI ACTAGT 2 cut(s) 383, 884
AluBI AGCT 7 cut(s) 47, 72, 219, 392, 543, 574, 707
AluI AGCT 7 cut(s) 47, 72, 219, 392, 543, 574, 707
Alw26I GTCTC 5 cut(s) 296, 325, 424, 752, 768
AlwI GGATC 1 cut(s) 799
AoxI GGCC 1 cut(s) 521
ApeKI GCWGC 3 cut(s) 452, 574, 786
ApoI RAATTY 1 cut(s) 878
Asp700I GAANNNNTTC 2 cut(s) 32, 108
AspLEI GCGC 1 cut(s) 263
BauI CACGAG 1 cut(s) 775
BbvI GCAGC 3 cut(s) 439, 561, 798
BccI CCATC 4 cut(s) 64, 251, 513, 577
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BclI TGATCA 3 cut(s) 7, 64, 555
BcoDI GTCTC 5 cut(s) 296, 325, 424, 752, 768
BcuI ACTAGT 2 cut(s) 383, 884
BfaI CTAG 4 cut(s) 44, 384, 801, 885
BfmI CTRYAG 2 cut(s) 575, 611
BfoI RGCGCY 1 cut(s) 264
BfrI CTTAAG 1 cut(s) 76
BglII AGATCT 1 cut(s) 106
BisI GCNGC 3 cut(s) 453, 575, 787
BlsI GCNGC 3 cut(s) 454, 576, 788
BmrI ACTGGG 1 cut(s) 106
BmsI GCATC 1 cut(s) 618
BmuI ACTGGG 1 cut(s) 106
BpuEI CTTGAG 5 cut(s) 123, 653, 734, 750, 856
Bse1I ACTGG 1 cut(s) 101
BseGI GGATG 1 cut(s) 56
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 3 cut(s) 439, 561, 798
Bsh1236I CGCG 2 cut(s) 501, 620
BshFI GGCC 1 cut(s) 523
BsiSI CCGG 1 cut(s) 665
BsmAI GTCTC 5 cut(s) 296, 325, 424, 752, 768
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 1 cut(s) 523
Bsp143I GATC 7 cut(s) 7, 64, 106, 178, 555, 757, 791
Bsp68I TCGCGA 1 cut(s) 501
BspACI CCGC 1 cut(s) 789
BspANI GGCC 1 cut(s) 523
BspCNI CTCAG 1 cut(s) 460
BspFNI CGCG 2 cut(s) 501, 620
BspMAI CTGCAG 2 cut(s) 579, 615
BspPI GGATC 1 cut(s) 799
BspTI CTTAAG 1 cut(s) 76
BsrI ACTGG 1 cut(s) 101
BssMI GATC 7 cut(s) 7, 64, 106, 178, 555, 757, 791
BssSI CACGAG 1 cut(s) 775
Bst2BI CACGAG 1 cut(s) 775
Bst4CI ACNGT 2 cut(s) 295, 418
Bst6I CTCTTC 5 cut(s) 272, 308, 588, 638, 734
BstAFI CTTAAG 1 cut(s) 76
BstDEI CTNAG 3 cut(s) 309, 447, 698
BstF5I GGATG 1 cut(s) 56
BstFNI CGCG 2 cut(s) 501, 620
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 7 cut(s) 10, 67, 109, 181, 558, 760, 794
BstMAI GTCTC 5 cut(s) 296, 325, 424, 752, 768
BstMBI GATC 7 cut(s) 7, 64, 106, 178, 555, 757, 791
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNSI RCATGY 1 cut(s) 439
BstSFI CTRYAG 2 cut(s) 575, 611
BstUI CGCG 2 cut(s) 501, 620
BstV1I GCAGC 3 cut(s) 439, 561, 798
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BsuRI GGCC 1 cut(s) 523
BtsCI GGATG 1 cut(s) 56
BtsIMutI CAGTG 1 cut(s) 300
BtuMI TCGCGA 1 cut(s) 501
CfoI GCGC 1 cut(s) 263
CseI GACGC 2 cut(s) 213, 626
Csp6I GTAC 2 cut(s) 203, 770
CviAII CATG 2 cut(s) 228, 436
CviQI GTAC 2 cut(s) 203, 770
DdeI CTNAG 3 cut(s) 309, 447, 698
DpnI GATC 7 cut(s) 9, 66, 108, 180, 557, 759, 793
DpnII GATC 7 cut(s) 7, 64, 106, 178, 555, 757, 791
Eam1104I CTCTTC 5 cut(s) 272, 308, 588, 638, 734
EarI CTCTTC 5 cut(s) 272, 308, 588, 638, 734
Eco57I CTGAAG 1 cut(s) 216
FaeI CATG 2 cut(s) 231, 439
FaiI YATR 6 cut(s) 214, 229, 345, 437, 443, 539
FatI CATG 2 cut(s) 227, 435
FbaI TGATCA 3 cut(s) 7, 64, 555
Fnu4HI GCNGC 3 cut(s) 453, 575, 787
FokI GGATG 1 cut(s) 43
Fsp4HI GCNGC 3 cut(s) 453, 575, 787
FspBI CTAG 4 cut(s) 44, 384, 801, 885
GlaI GCGC 1 cut(s) 262
GluI GCNGC 3 cut(s) 453, 575, 787
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 1 cut(s) 523
HapII CCGG 1 cut(s) 665
HgaI GACGC 2 cut(s) 213, 626
HhaI GCGC 1 cut(s) 263
Hin1II CATG 2 cut(s) 231, 439
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 705
HinfI GANTC 2 cut(s) 354, 809
HpaII CCGG 1 cut(s) 665
Hpy166II GTNNAC 1 cut(s) 533
Hpy188I TCNGA 3 cut(s) 196, 271, 757
Hpy188III TCNNGA 2 cut(s) 182, 500
Hpy8I GTNNAC 1 cut(s) 533
HpyAV CCTTC 2 cut(s) 581, 631
HpyCH4III ACNGT 2 cut(s) 295, 418
HpyCH4IV ACGT 2 cut(s) 468, 564
HpyCH4V TGCA 4 cut(s) 455, 577, 602, 613
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 3 cut(s) 309, 447, 698
HpySE526I ACGT 2 cut(s) 468, 564
Hsp92II CATG 2 cut(s) 231, 439
HspAI GCGC 1 cut(s) 261
Ksp22I TGATCA 3 cut(s) 7, 64, 555
Kzo9I GATC 7 cut(s) 7, 64, 106, 178, 555, 757, 791
LmnI GCTCC 3 cut(s) 77, 397, 571
LpnPI CCDG 7 cut(s) 82, 117, 189, 434, 599, 678, 810
Lsp1109I GCAGC 3 cut(s) 439, 561, 798
LweI GCATC 1 cut(s) 618
MaeI CTAG 4 cut(s) 44, 384, 801, 885
MaeII ACGT 2 cut(s) 468, 564
MaeIII GTNAC 1 cut(s) 560
MalI GATC 7 cut(s) 9, 66, 108, 180, 557, 759, 793
MboI GATC 7 cut(s) 7, 64, 106, 178, 555, 757, 791
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 1 cut(s) 106
MluCI AATT 4 cut(s) 208, 503, 651, 878
MluI ACGCGT 1 cut(s) 618
MlyI GAGTC 1 cut(s) 818
MmeI TCCRAC 2 cut(s) 419, 603
MnlI CCTC 9 cut(s) 245, 273, 309, 534, 639, 649, 687, 723, 784
MroXI GAANNNNTTC 2 cut(s) 32, 108
MseI TTAA 5 cut(s) 77, 465, 476, 545, 678
MspA1I CMGCKG 1 cut(s) 789
MspCI CTTAAG 1 cut(s) 76
MspI CCGG 1 cut(s) 665
MunI CAATTG 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 604
MvnI CGCG 2 cut(s) 501, 620
MwoI GCNNNNNNNGC 1 cut(s) 458
NdeII GATC 7 cut(s) 7, 64, 106, 178, 555, 757, 791
NlaIII CATG 2 cut(s) 231, 439
NruI TCGCGA 1 cut(s) 501
NspI RCATGY 1 cut(s) 439
PctI GAATGC 1 cut(s) 604
PdmI GAANNNNTTC 2 cut(s) 32, 108
PfeI GAWTC 1 cut(s) 354
PflFI GACNNNGTC 1 cut(s) 403
PkrI GCNGC 3 cut(s) 454, 576, 788
PleI GAGTC 1 cut(s) 817
PpsI GAGTC 1 cut(s) 817
Psp1406I AACGTT 1 cut(s) 468
PstI CTGCAG 2 cut(s) 579, 615
PsuI RGATCY 1 cut(s) 106
PsyI GACNNNGTC 1 cut(s) 403
RruI TCGCGA 1 cut(s) 501
RsaI GTAC 2 cut(s) 204, 771
RsaNI GTAC 2 cut(s) 203, 770
SaqAI TTAA 5 cut(s) 77, 465, 476, 545, 678
SatI GCNGC 3 cut(s) 453, 575, 787
Sau3AI GATC 7 cut(s) 7, 64, 106, 178, 555, 757, 791
SchI GAGTC 1 cut(s) 818
SfaNI GCATC 1 cut(s) 618
SfcI CTRYAG 2 cut(s) 575, 611
SmlI CTYRAG 6 cut(s) 76, 138, 632, 749, 765, 871
SmoI CTYRAG 6 cut(s) 76, 138, 632, 749, 765, 871
SpeI ACTAGT 2 cut(s) 383, 884
Sse9I AATT 4 cut(s) 208, 503, 651, 878
SsiI CCGC 1 cut(s) 789
SspMI CTAG 4 cut(s) 44, 384, 801, 885
TaaI ACNGT 2 cut(s) 295, 418
TaiI ACGT 2 cut(s) 471, 567
TaqI TCGA 1 cut(s) 93
TasI AATT 4 cut(s) 208, 503, 651, 878
TatI WGTACW 1 cut(s) 202
TfiI GAWTC 1 cut(s) 354
Tru1I TTAA 5 cut(s) 77, 465, 476, 545, 678
Tru9I TTAA 5 cut(s) 77, 465, 476, 545, 678
TscAI CASTG 1 cut(s) 300
TseI GCWGC 3 cut(s) 452, 574, 786
TspDTI ATGAA 5 cut(s) 201, 255, 834, 855, 863
TspRI CASTG 1 cut(s) 300
Tth111I GACNNNGTC 1 cut(s) 403
Vha464I CTTAAG 1 cut(s) 76
XapI RAATTY 1 cut(s) 878
XceI RCATGY 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XmnI GAANNNNTTC 2 cut(s) 32, 108
XspI CTAG 4 cut(s) 44, 384, 801, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.