Rmu_sc0002060.1_g000022

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002060.1
OG0012997
Physical Location & Seq
Forward (+)
88414 .. 89798
1385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002060.1_g000022.1.cds

Sequence Viewer

Length: 1074 bp
atgacaatgaagagtcaccatcagagtcgttccctgacgcctaattgggtagtttctaaggatcctgaaaacccattctcattcccaaatcaattttctcctgctgatttcagtgggtatgctccggtacttgattgggaaaatgaatttccgccgatggtgcaagactacacttttattgatgctcttcctttcatggagagttgtcttccttcgctcgatattcaacctatccgacacctatttccaggagatgatttttcctttggagataatggaaatgagaatgagaatggagttaacagtgagttgttcgcttctgggtttgagcgccctaatcatcatcatcaacaagaacaagaacaagaacagcagcagcagcagcaacaacaacatcaacaacaagaaaagcagcaacctaatttactgcttcctagcaatgaagatcaaagtggtgctgatagagaacatgtggctgaagagaggaagaggaagaggcggtgcatgagaattgatcacgaagtgaaaagtggtagtagtactagaagctattcatcgtcgagattgttgtctagggaaacaatctcctcgtacttttacatgcctataactcaggcagctaaggaactcaacatcggcttgacccttttgaagaaaaggtgccgggacttgggtattcgtcgttggcctcatcggaagctcaccagcctccaaaccctaatccggaacattcaggagttgggaaaggaagaagaggagaacgaagagaagcttcggaatgcgatcgagttgttggagagggagaagaagttgatggaggaagctcctgatatgcaactggaggataataccaagcggttgagacaggcttgttttaaggccaactacaagaagagaaagatcatggggatgaatctgattgattatccaccgcattcttctaatttcagcaccattgatactcgtggaggcaagtccgtcgctattcttgacaatcgaggagacgaggaggacgaagagattaagtcacttttatctgactccttctcatcttcctctagcatgatgatataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

357

Amino Acids

41.47

Weight (kDa)

5.57

Isoelectric Point (pI)

72.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 660
AccIII TCCGGA 1 cut(s) 723
AciI CCGC 4 cut(s) 152, 499, 856, 932
AclWI GGATC 2 cut(s) 56, 69
AcsI RAATTY 1 cut(s) 146
AcuI CTGAAG 1 cut(s) 498
AcyI GRCGYC 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 523
AfaI GTAC 3 cut(s) 129, 541, 593
AfiI CCNNNNNNNGG 2 cut(s) 670, 723
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 2 cut(s) 227, 652
AjnI CCWGG 1 cut(s) 247
AjuI GAANNNNNNNTTGG 2 cut(s) 79, 111
AluBI AGCT 5 cut(s) 549, 620, 700, 772, 824
AluI AGCT 5 cut(s) 549, 620, 700, 772, 824
Alw26I GTCTC 2 cut(s) 856, 996
AlwI GGATC 2 cut(s) 56, 69
Aor13HI TCCGGA 1 cut(s) 723
AoxI GGCC 2 cut(s) 686, 879
ApeKI GCWGC 6 cut(s) 373, 376, 379, 382, 412, 617
ApoI RAATTY 1 cut(s) 146
Asp700I GAANNNNTTC 1 cut(s) 550
AspLEI GCGC 1 cut(s) 333
AsuC2I CCSGG 1 cut(s) 665
AsuHPI GGTGA 2 cut(s) 8, 694
BamHI GGATCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 660
BauI CACGAG 1 cut(s) 963
BbsI GAAGAC 1 cut(s) 200
BbvI GCAGC 6 cut(s) 385, 388, 391, 394, 424, 629
BccI CCATC 3 cut(s) 27, 151, 808
BcgI CGANNNNNNTGC 2 cut(s) 961, 995
BciT130I CCWGG 1 cut(s) 249
BclI TGATCA 1 cut(s) 514
BcnI CCSGG 1 cut(s) 665
BcoDI GTCTC 2 cut(s) 856, 996
BfaI CTAG 4 cut(s) 435, 543, 573, 1059
BfoI RGCGCY 1 cut(s) 334
BisI GCNGC 6 cut(s) 374, 377, 380, 383, 413, 618
BlsI GCNGC 6 cut(s) 375, 378, 381, 384, 414, 619
BmcAI AGTACT 1 cut(s) 541
Bme1390I CCNGG 2 cut(s) 249, 665
BmiI GGNNCC 2 cut(s) 63, 662
BmrFI CCNGG 2 cut(s) 249, 665
BmsI GCATC 1 cut(s) 172
BpiI GAAGAC 1 cut(s) 200
BpmI CTGGAG 1 cut(s) 860
Bpu10I CCTNAGC 1 cut(s) 621
BpuMI CCSGG 1 cut(s) 665
BsaHI GRCGYC 1 cut(s) 38
BsaWI WCCGGW 2 cut(s) 124, 723
BsaXI ACNNNNNCTCC 2 cut(s) 794, 824
Bsc4I CCNNNNNNNGG 2 cut(s) 670, 723
Bse1I ACTGG 1 cut(s) 843
Bse3DI GCAATG 1 cut(s) 445
BseAI TCCGGA 1 cut(s) 723
BseBI CCWGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 915
BseLI CCNNNNNNNGG 2 cut(s) 670, 723
BseMI GCAATG 1 cut(s) 445
BseMII CTCAG 1 cut(s) 626
BseNI ACTGG 1 cut(s) 843
BseRI GAGGAG 4 cut(s) 577, 770, 1014, 1022
BseXI GCAGC 6 cut(s) 385, 388, 391, 394, 424, 629
Bsh1285I CGRYCG 1 cut(s) 786
BshFI GGCC 2 cut(s) 688, 881
BshNI GGYRCC 1 cut(s) 660
BsiEI CGRYCG 1 cut(s) 786
BsiSI CCGG 3 cut(s) 125, 664, 724
BslFI GGGAC 1 cut(s) 680
BslI CCNNNNNNNGG 2 cut(s) 670, 723
BsmAI GTCTC 2 cut(s) 856, 996
BsmBI CGTCTC 1 cut(s) 996
BsmFI GGGAC 1 cut(s) 680
BsmI GAATGC 2 cut(s) 784, 934
BsnI GGCC 2 cut(s) 688, 881
Bsp13I TCCGGA 1 cut(s) 723
Bsp143I GATC 5 cut(s) 61, 445, 514, 783, 900
BspACI CCGC 4 cut(s) 152, 499, 856, 932
BspANI GGCC 2 cut(s) 688, 881
BspCNI CTCAG 1 cut(s) 625
BspEI TCCGGA 1 cut(s) 723
BspLI GGNNCC 2 cut(s) 63, 662
BspPI GGATC 2 cut(s) 56, 69
BspQI GCTCTTC 1 cut(s) 192
BspT107I GGYRCC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 445
BsrI ACTGG 1 cut(s) 843
BssMI GATC 5 cut(s) 61, 445, 514, 783, 900
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 963
Bst2BI CACGAG 1 cut(s) 963
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 9 cut(s) 5, 192, 474, 482, 488, 747, 759, 887, 1011
BstACI GRCGYC 1 cut(s) 38
BstDEI CTNAG 3 cut(s) 57, 612, 621
BstF5I GGATG 1 cut(s) 915
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 5 cut(s) 64, 448, 517, 786, 903
BstMAI GTCTC 2 cut(s) 856, 996
BstMBI GATC 5 cut(s) 61, 445, 514, 783, 900
BstMCI CGRYCG 1 cut(s) 786
BstMWI GCNNNNNNNGC 3 cut(s) 160, 379, 382
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 2 cut(s) 473, 604
BstSCI CCNGG 2 cut(s) 247, 663
BstV1I GCAGC 6 cut(s) 385, 388, 391, 394, 424, 629
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 2 cut(s) 688, 881
BtsCI GGATG 1 cut(s) 915
BtsIMutI CAGTG 2 cut(s) 118, 310
CfoI GCGC 1 cut(s) 333
CseI GACGC 1 cut(s) 46
Csp6I GTAC 3 cut(s) 128, 540, 592
CviAII CATG 6 cut(s) 196, 470, 505, 601, 904, 1063
CviQI GTAC 3 cut(s) 128, 540, 592
DdeI CTNAG 3 cut(s) 57, 612, 621
DpnI GATC 5 cut(s) 63, 447, 516, 785, 902
DpnII GATC 5 cut(s) 61, 445, 514, 783, 900
DraIII CACNNNGTG 1 cut(s) 523
Eam1104I CTCTTC 9 cut(s) 5, 192, 474, 482, 488, 747, 759, 887, 1011
EarI CTCTTC 9 cut(s) 5, 192, 474, 482, 488, 747, 759, 887, 1011
EciI GGCGGA 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 498
EcoRII CCWGG 1 cut(s) 247
Esp3I CGTCTC 1 cut(s) 996
FaeI CATG 6 cut(s) 199, 473, 508, 604, 907, 1066
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 1 cut(s) 680
FatI CATG 6 cut(s) 195, 469, 504, 600, 903, 1062
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 6 cut(s) 374, 377, 380, 383, 413, 618
FokI GGATG 1 cut(s) 922
Fsp4HI GCNGC 6 cut(s) 374, 377, 380, 383, 413, 618
FspBI CTAG 4 cut(s) 435, 543, 573, 1059
GlaI GCGC 1 cut(s) 332
GluI GCNGC 6 cut(s) 374, 377, 380, 383, 413, 618
GsuI CTGGAG 1 cut(s) 860
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 2 cut(s) 688, 881
HapII CCGG 3 cut(s) 125, 664, 724
HgaI GACGC 1 cut(s) 46
HhaI GCGC 1 cut(s) 333
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 6 cut(s) 199, 473, 508, 604, 907, 1066
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 770
HinfI GANTC 4 cut(s) 13, 25, 913, 1040
HpaI GTTAAC 1 cut(s) 301
HpaII CCGG 3 cut(s) 125, 664, 724
HphI GGTGA 2 cut(s) 8, 694
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 6 cut(s) 24, 236, 696, 777, 918, 1039
Hpy188III TCNNGA 7 cut(s) 65, 518, 561, 724, 734, 827, 989
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 3 cut(s) 562, 684, 983
HpyAV CCTTC 2 cut(s) 222, 1054
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4V TGCA 3 cut(s) 163, 504, 835
HpyF10VI GCNNNNNNNGC 3 cut(s) 160, 379, 382
HpyF3I CTNAG 3 cut(s) 57, 612, 621
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 6 cut(s) 199, 473, 508, 604, 907, 1066
HspAI GCGC 1 cut(s) 331
Kpn2I TCCGGA 1 cut(s) 723
Ksp22I TGATCA 1 cut(s) 514
KspAI GTTAAC 1 cut(s) 301
Kzo9I GATC 5 cut(s) 61, 445, 514, 783, 900
LguI GCTCTTC 1 cut(s) 192
LmnI GCTCC 2 cut(s) 127, 829
Lsp1109I GCAGC 6 cut(s) 385, 388, 391, 394, 424, 629
LweI GCATC 1 cut(s) 172
MaeI CTAG 4 cut(s) 435, 543, 573, 1059
MaeIII GTNAC 2 cut(s) 14, 1026
MalI GATC 5 cut(s) 63, 447, 516, 785, 902
MboI GATC 5 cut(s) 61, 445, 514, 783, 900
MflI RGATCY 1 cut(s) 61
MluCI AATT 6 cut(s) 43, 92, 146, 421, 510, 943
MlyI GAGTC 3 cut(s) 22, 34, 1034
MmeI TCCRAC 2 cut(s) 259, 774
MroI TCCGGA 1 cut(s) 723
MroXI GAANNNNTTC 1 cut(s) 550
MseI TTAA 3 cut(s) 300, 876, 1023
MslI CAYNNNNRTG 1 cut(s) 908
MspI CCGG 3 cut(s) 125, 664, 724
MspR9I CCNGG 2 cut(s) 249, 665
Mva1269I GAATGC 2 cut(s) 784, 934
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 3 cut(s) 160, 379, 382
NciI CCSGG 1 cut(s) 665
NdeII GATC 5 cut(s) 61, 445, 514, 783, 900
NlaIII CATG 6 cut(s) 199, 473, 508, 604, 907, 1066
NlaIV GGNNCC 2 cut(s) 63, 662
NmuCI GTSAC 2 cut(s) 14, 1026
NspI RCATGY 2 cut(s) 473, 604
PciI ACATGT 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 1011
PctI GAATGC 2 cut(s) 784, 934
PdmI GAANNNNTTC 1 cut(s) 550
PfeI GAWTC 1 cut(s) 913
PfoI TCCNGGA 1 cut(s) 247
PkrI GCNGC 6 cut(s) 375, 378, 381, 384, 414, 619
Ple19I CGATCG 1 cut(s) 786
PleI GAGTC 3 cut(s) 21, 33, 1034
PpsI GAGTC 3 cut(s) 21, 33, 1034
PscI ACATGT 1 cut(s) 469
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 2 cut(s) 63, 662
PsuI RGATCY 1 cut(s) 61
PvuI CGATCG 1 cut(s) 786
RsaI GTAC 3 cut(s) 129, 541, 593
RsaNI GTAC 3 cut(s) 128, 540, 592
RseI CAYNNNNRTG 1 cut(s) 908
SapI GCTCTTC 1 cut(s) 192
SaqAI TTAA 3 cut(s) 300, 876, 1023
SatI GCNGC 6 cut(s) 374, 377, 380, 383, 413, 618
Sau3AI GATC 5 cut(s) 61, 445, 514, 783, 900
ScaI AGTACT 1 cut(s) 541
SchI GAGTC 3 cut(s) 22, 34, 1034
ScrFI CCNGG 2 cut(s) 249, 665
SetI ASST 9 cut(s) 232, 243, 421, 551, 622, 662, 702, 774, 826
SfaNI GCATC 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 908
Sse9I AATT 6 cut(s) 43, 92, 146, 421, 510, 943
SsiI CCGC 4 cut(s) 152, 499, 856, 932
SspMI CTAG 4 cut(s) 435, 543, 573, 1059
StyD4I CCNGG 2 cut(s) 247, 663
TaaI ACNGT 1 cut(s) 305
TaqI TCGA 4 cut(s) 219, 560, 786, 997
TasI AATT 6 cut(s) 43, 92, 146, 421, 510, 943
TatI WGTACW 1 cut(s) 539
TfiI GAWTC 1 cut(s) 913
Tru1I TTAA 3 cut(s) 300, 876, 1023
Tru9I TTAA 3 cut(s) 300, 876, 1023
TscAI CASTG 2 cut(s) 118, 310
TseFI GTSAC 2 cut(s) 14, 1026
TseI GCWGC 6 cut(s) 373, 376, 379, 382, 412, 617
Tsp45I GTSAC 2 cut(s) 14, 1026
TspDTI ATGAA 6 cut(s) 23, 159, 184, 456, 543, 926
TspGWI ACGGA 1 cut(s) 967
TspRI CASTG 2 cut(s) 118, 310
XapI RAATTY 1 cut(s) 146
XceI RCATGY 2 cut(s) 473, 604
XmnI GAANNNNTTC 1 cut(s) 550
XspI CTAG 4 cut(s) 435, 543, 573, 1059
ZrmI AGTACT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.