RLG00000005191

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012997
Physical Location & Seq
Reverse (-)
65732152 .. 65733555
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005191

Sequence Viewer

Length: 1086 bp
ATGACAATGAAGAGTCACCATCAGAGTCGTTCCCTGACGAGTAACCCTAATTGGGTGGTTTCTAAGGATCATGAAAACCCATTCTCATTCCCAAATCAATTTTCTCCTGCTGATTTCAGTGGGTATGCTCCGGCACTTGATTGGGAAAATGAATTTCCGCCGATGGTGCAAGACTACACTTTTATTGATGCTCTTCCTTTCATGGAGAGTTGTCTTCCTTCGCTCGATATTCAACCTATCCGAAGCGTATTTCCAGGAGATGATTTTTCCTTTGGAGATAATGGAAATGAGACTGAGAATGGAGTTAACAGTGAGTTGTTCGCTTCTGGGTTTGAGCGCCCTAATCATCATTATCAACAAGAACAAGAACAACAGCAGCAGCAGCAGCAGCAGCAAAAACAACTTCAACAACAAGAAAAGCAGCAACCTAATTTACTGCTTCCTAGCAATGAAGATCAAAGTGGTGCTGATAGAGAACATGTGGGTGAAGAGAGGAAGAGGAAGAGGCGGTGCATGAGAATTGATCATGAAGTGAAGAGTGGTAGTAGTACTAGAAGCTATTCATCGTCGAGGTTGTTGTCTAGGGAAACAATCTCCTCGTACTTTTACATGCCTATAACTCAGGCAGCTAAGGAACTCAACATCGGCTTGACCCTTTTGAAGAAAAGGTGCCGGGAGTTGGGTATTCGTCGTTGGCCTCATCGGAAACTCACCAGCCTCCAAACCCTAATCCGGAACATTCAGGAGTTGGGAAAGGAAGAAGAGGAGAACGAAGAGAAGCTTCGGAATGCGATCGAGTTGTTGGAGAGGGAGAAGAAGTTGATGGAGGAAGCTCCTGATATGCAACTGGAGGATAATACCAAGCGGTTGAGACAGGCTTGTTTTAAGGCCAACTACAAGAAGAGAAAGATCATGGGGATGAATCTGATTGATTATCCACCCCATTCTTCTAAATATTTCAGCACCATTGATACTCGTGGAGGCAAGTCCATCGCTATTCTTGACAATCGAGGAGACGAGGAAGATGAAGAGATTAAGTCACTTTTATCTGACTCCTTCTCATCTTCCTCTAGCATGATGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

362

Amino Acids

41.92

Weight (kDa)

5.72

Isoelectric Point (pI)

74.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 193 - 240 1.6e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 669
AccIII TCCGGA 1 cut(s) 732
AciI CCGC 3 cut(s) 158, 508, 865
AclWI GGATC 1 cut(s) 75
AcsI RAATTY 1 cut(s) 152
AfaI GTAC 2 cut(s) 550, 602
AfiI CCNNNNNNNGG 3 cut(s) 52, 679, 732
AflIII ACRYGT 1 cut(s) 478
AgsI TTSAA 3 cut(s) 233, 407, 661
AjnI CCWGG 1 cut(s) 253
AjuI GAANNNNNNNTTGG 2 cut(s) 85, 117
AluBI AGCT 4 cut(s) 558, 629, 781, 833
AluI AGCT 4 cut(s) 558, 629, 781, 833
Alw26I GTCTC 3 cut(s) 284, 865, 1008
AlwI GGATC 1 cut(s) 75
Aor13HI TCCGGA 1 cut(s) 732
AoxI GGCC 2 cut(s) 695, 888
ApeKI GCWGC 8 cut(s) 376, 379, 382, 385, 388, 391, 421, 626
ApoI RAATTY 1 cut(s) 152
Asp700I GAANNNNTTC 1 cut(s) 559
AspLEI GCGC 1 cut(s) 339
AsuC2I CCSGG 1 cut(s) 674
AsuHPI GGTGA 3 cut(s) 8, 497, 703
BanI GGYRCC 1 cut(s) 669
BauI CACGAG 1 cut(s) 975
BbsI GAAGAC 1 cut(s) 206
BbvI GCAGC 8 cut(s) 388, 391, 394, 397, 400, 403, 433, 638
BccI CCATC 4 cut(s) 27, 157, 817, 998
BcgI CGANNNNNNTGC 2 cut(s) 973, 1007
BciT130I CCWGG 1 cut(s) 255
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 1 cut(s) 674
BcoDI GTCTC 3 cut(s) 284, 865, 1008
BfaI CTAG 4 cut(s) 444, 552, 582, 1071
BfoI RGCGCY 1 cut(s) 340
BisI GCNGC 8 cut(s) 377, 380, 383, 386, 389, 392, 422, 627
BlsI GCNGC 8 cut(s) 378, 381, 384, 387, 390, 393, 423, 628
BmcAI AGTACT 1 cut(s) 550
Bme1390I CCNGG 2 cut(s) 255, 674
BmiI GGNNCC 1 cut(s) 671
BmrFI CCNGG 2 cut(s) 255, 674
BmsI GCATC 1 cut(s) 178
BpiI GAAGAC 1 cut(s) 206
BpmI CTGGAG 1 cut(s) 869
Bpu10I CCTNAGC 1 cut(s) 630
BpuMI CCSGG 1 cut(s) 674
BsaWI WCCGGW 1 cut(s) 732
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 679, 732
Bse1I ACTGG 1 cut(s) 852
Bse3DI GCAATG 1 cut(s) 454
BseAI TCCGGA 1 cut(s) 732
BseBI CCWGG 1 cut(s) 255
BseGI GGATG 1 cut(s) 924
BseLI CCNNNNNNNGG 3 cut(s) 52, 679, 732
BseMI GCAATG 1 cut(s) 454
BseMII CTCAG 2 cut(s) 285, 635
BseNI ACTGG 1 cut(s) 852
BseRI GAGGAG 3 cut(s) 586, 779, 1026
BseXI GCAGC 8 cut(s) 388, 391, 394, 397, 400, 403, 433, 638
Bsh1285I CGRYCG 1 cut(s) 795
BshFI GGCC 2 cut(s) 697, 890
BshNI GGYRCC 1 cut(s) 669
BsiEI CGRYCG 1 cut(s) 795
BsiSI CCGG 3 cut(s) 131, 673, 733
BslI CCNNNNNNNGG 3 cut(s) 52, 679, 732
BsmAI GTCTC 3 cut(s) 284, 865, 1008
BsmBI CGTCTC 1 cut(s) 1008
BsmI GAATGC 1 cut(s) 793
BsnI GGCC 2 cut(s) 697, 890
Bsp13I TCCGGA 1 cut(s) 732
Bsp143I GATC 5 cut(s) 67, 454, 523, 792, 909
BspACI CCGC 3 cut(s) 158, 508, 865
BspANI GGCC 2 cut(s) 697, 890
BspCNI CTCAG 2 cut(s) 286, 634
BspEI TCCGGA 1 cut(s) 732
BspHI TCATGA 2 cut(s) 70, 526
BspLI GGNNCC 1 cut(s) 671
BspPI GGATC 1 cut(s) 75
BspQI GCTCTTC 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 669
BsrDI GCAATG 1 cut(s) 454
BsrI ACTGG 1 cut(s) 852
BssMI GATC 5 cut(s) 67, 454, 523, 792, 909
BssSI CACGAG 1 cut(s) 975
Bst2BI CACGAG 1 cut(s) 975
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 311
BstDEI CTNAG 4 cut(s) 63, 294, 621, 630
BstF5I GGATG 1 cut(s) 924
BstH2I RGCGCY 1 cut(s) 340
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 5 cut(s) 70, 457, 526, 795, 912
BstMAI GTCTC 3 cut(s) 284, 865, 1008
BstMBI GATC 5 cut(s) 67, 454, 523, 792, 909
BstMCI CGRYCG 1 cut(s) 795
BstMWI GCNNNNNNNGC 5 cut(s) 166, 382, 385, 388, 391
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 2 cut(s) 482, 613
BstSCI CCNGG 2 cut(s) 253, 672
BstV1I GCAGC 8 cut(s) 388, 391, 394, 397, 400, 403, 433, 638
BstV2I GAAGAC 1 cut(s) 206
BsuRI GGCC 2 cut(s) 697, 890
BtgZI GCGATG 1 cut(s) 976
BtsCI GGATG 1 cut(s) 924
BtsIMutI CAGTG 2 cut(s) 124, 316
CciI TCATGA 2 cut(s) 70, 526
CfoI GCGC 1 cut(s) 339
Csp6I GTAC 2 cut(s) 549, 601
CviAII CATG 8 cut(s) 71, 202, 479, 514, 527, 610, 913, 1075
CviJI RGCY 9 cut(s) 558, 629, 648, 697, 717, 781, 833, 878, 890
CviKI_1 RGCY 9 cut(s) 558, 629, 648, 697, 717, 781, 833, 878, 890
CviQI GTAC 2 cut(s) 549, 601
DdeI CTNAG 4 cut(s) 63, 294, 621, 630
DpnI GATC 5 cut(s) 69, 456, 525, 794, 911
DpnII GATC 5 cut(s) 67, 454, 523, 792, 909
EciI GGCGGA 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 1008
FaeI CATG 8 cut(s) 74, 205, 482, 517, 530, 613, 916, 1078
FalI AAGNNNNNCTT 2 cut(s) 765, 797
FatI CATG 8 cut(s) 70, 201, 478, 513, 526, 609, 912, 1074
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 8 cut(s) 377, 380, 383, 386, 389, 392, 422, 627
FokI GGATG 1 cut(s) 931
Fsp4HI GCNGC 8 cut(s) 377, 380, 383, 386, 389, 392, 422, 627
FspBI CTAG 4 cut(s) 444, 552, 582, 1071
GlaI GCGC 1 cut(s) 338
GluI GCNGC 8 cut(s) 377, 380, 383, 386, 389, 392, 422, 627
GsuI CTGGAG 1 cut(s) 869
HaeII RGCGCY 1 cut(s) 340
HaeIII GGCC 2 cut(s) 697, 890
HapII CCGG 3 cut(s) 131, 673, 733
HhaI GCGC 1 cut(s) 339
Hin1II CATG 8 cut(s) 74, 205, 482, 517, 530, 613, 916, 1078
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 4 cut(s) 13, 25, 922, 1052
HpaI GTTAAC 1 cut(s) 307
HpaII CCGG 3 cut(s) 131, 673, 733
HphI GGTGA 3 cut(s) 8, 497, 703
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 6 cut(s) 24, 242, 705, 786, 927, 1051
Hpy188III TCNNGA 6 cut(s) 71, 527, 733, 743, 836, 1001
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 2 cut(s) 571, 693
HpyAV CCTTC 2 cut(s) 228, 1066
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4V TGCA 3 cut(s) 169, 513, 844
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 382, 385, 388, 391
HpyF3I CTNAG 4 cut(s) 63, 294, 621, 630
Hsp92II CATG 8 cut(s) 74, 205, 482, 517, 530, 613, 916, 1078
HspAI GCGC 1 cut(s) 337
Kpn2I TCCGGA 1 cut(s) 732
Ksp22I TGATCA 1 cut(s) 523
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 5 cut(s) 67, 454, 523, 792, 909
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 2 cut(s) 133, 838
Lsp1109I GCAGC 8 cut(s) 388, 391, 394, 397, 400, 403, 433, 638
LweI GCATC 1 cut(s) 178
MaeI CTAG 4 cut(s) 444, 552, 582, 1071
MaeIII GTNAC 3 cut(s) 14, 41, 1038
MalI GATC 5 cut(s) 69, 456, 525, 794, 911
MboI GATC 5 cut(s) 67, 454, 523, 792, 909
MluCI AATT 5 cut(s) 49, 98, 152, 430, 519
MlyI GAGTC 3 cut(s) 22, 34, 1046
MmeI TCCRAC 1 cut(s) 783
MroI TCCGGA 1 cut(s) 732
MroXI GAANNNNTTC 1 cut(s) 559
MseI TTAA 3 cut(s) 306, 885, 1035
MslI CAYNNNNRTG 2 cut(s) 483, 917
MspI CCGG 3 cut(s) 131, 673, 733
MspR9I CCNGG 2 cut(s) 255, 674
Mva1269I GAATGC 1 cut(s) 793
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 5 cut(s) 166, 382, 385, 388, 391
NciI CCSGG 1 cut(s) 674
NdeII GATC 5 cut(s) 67, 454, 523, 792, 909
NlaIII CATG 8 cut(s) 74, 205, 482, 517, 530, 613, 916, 1078
NlaIV GGNNCC 1 cut(s) 671
NmuCI GTSAC 2 cut(s) 14, 1038
NspI RCATGY 2 cut(s) 482, 613
PagI TCATGA 2 cut(s) 70, 526
PciI ACATGT 1 cut(s) 478
PciSI GCTCTTC 1 cut(s) 198
PctI GAATGC 1 cut(s) 793
PdmI GAANNNNTTC 1 cut(s) 559
PfeI GAWTC 1 cut(s) 922
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 8 cut(s) 378, 381, 384, 387, 390, 393, 423, 628
Ple19I CGATCG 1 cut(s) 795
PleI GAGTC 3 cut(s) 21, 33, 1046
PpsI GAGTC 3 cut(s) 21, 33, 1046
PscI ACATGT 1 cut(s) 478
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 671
PvuI CGATCG 1 cut(s) 795
RsaI GTAC 2 cut(s) 550, 602
RsaNI GTAC 2 cut(s) 549, 601
RseI CAYNNNNRTG 2 cut(s) 483, 917
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 3 cut(s) 306, 885, 1035
SatI GCNGC 8 cut(s) 377, 380, 383, 386, 389, 392, 422, 627
Sau3AI GATC 5 cut(s) 67, 454, 523, 792, 909
ScaI AGTACT 1 cut(s) 550
SchI GAGTC 3 cut(s) 22, 34, 1046
ScrFI CCNGG 2 cut(s) 255, 674
SetI ASST 8 cut(s) 238, 430, 560, 575, 631, 671, 783, 835
SfaNI GCATC 1 cut(s) 178
SmiMI CAYNNNNRTG 2 cut(s) 483, 917
Sse9I AATT 5 cut(s) 49, 98, 152, 430, 519
SsiI CCGC 3 cut(s) 158, 508, 865
SspI AATATT 1 cut(s) 956
SspMI CTAG 4 cut(s) 444, 552, 582, 1071
StyD4I CCNGG 2 cut(s) 253, 672
TaaI ACNGT 1 cut(s) 311
TaqI TCGA 4 cut(s) 225, 569, 795, 1009
TasI AATT 5 cut(s) 49, 98, 152, 430, 519
TatI WGTACW 1 cut(s) 548
TfiI GAWTC 1 cut(s) 922
Tru1I TTAA 3 cut(s) 306, 885, 1035
Tru9I TTAA 3 cut(s) 306, 885, 1035
TscAI CASTG 2 cut(s) 124, 316
TseFI GTSAC 2 cut(s) 14, 1038
TseI GCWGC 8 cut(s) 376, 379, 382, 385, 388, 391, 421, 626
Tsp45I GTSAC 2 cut(s) 14, 1038
TspDTI ATGAA 9 cut(s) 23, 87, 165, 190, 465, 543, 552, 935, 1041
TspRI CASTG 2 cut(s) 124, 316
XapI RAATTY 1 cut(s) 152
XceI RCATGY 2 cut(s) 482, 613
XmnI GAANNNNTTC 1 cut(s) 559
XspI CTAG 4 cut(s) 444, 552, 582, 1071
ZrmI AGTACT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.