Rh7DG043200

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012997
Physical Location & Seq
Forward (+)
3290389 .. 3291767
1379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG043200.1

Sequence Viewer

Length: 1068 bp
ATGACAATGAAGAGTCACCATCAGAGTCGTTCCCTGACGCCTAATTGGGTAGTTTCTAAGGATCCTGAAAACCCATTCTCATTCCCAAATCAATTTTCTCCTGCTGATTTCAGTGGGTATGCTCCGGTACTTGATTGGGAAAATGAATTTCCGCCGATGGTGCAAGACTACACTTTTATTGATGCTCTTCCTTTCATGGAGAGTTGTCTTCCTTCGCTCGATATTCAACCTATCCGACACCTATTTCCAGGAGATGATTTTTCCTTTGGAGATAATGGAAATGAGACTGAGAATGGAGTTAACAGTGAGTTGTTCGCTTCTGGGTTTGAGCGCCCTAATCATCATCATCAACAAGAACAAGAACAACAACAGCAGCAGCAGCAGCAACAGCAACAACAAGAAAAGCAGCAACCTAATTTACTGCTTCCTAGCAATGAAGATCAAAGTGGTGCTGATAGAGAACATGTGGCTGAAGAGAGGAAGAGGAAGAGGCGGTGCATGAGAATTGATCATGAAGTGAAAAGTGGTAGTAGTACTAGAAGCTATTCATCGTCGAGATTGTTGTCTAGGGAAACAATCTCCTCGTACTTTTACATGCCTATAACTCAGGCAGCTAAGGAACTCAACATCGGCTTGACCCTTTTGAAGAAAAGGTGCAGGGAGTTGGGTATTCGTCGTTGGCCTCATCGGAAACTCACCAGCCTCCAAACCCTAATCCGGAACATTCAGGAGTTGGGAAAGGAAGAAGAGGAGAACGAAGAGAAGCTTCGGAATGCGATCGAGTTGTTGGAGAGGGAGAAGAAGTTGATGGAGGAAGCTCCTGATATGCAACTGGAGGATAATACCAAGCGGTTGAGACAGGCTTGTTTTAAGGCCAACTACAAGAAGAGAAAGATCATGGGGATGAATCTGATTGATTATCCACCGCATTCTTCTAATTTCAGCACCATTGATACTCGTGGAGGCAAGTCCGTCGCTATTCTTGACAATCGAGGAGAGGAGGAGGACGAAGAGATTAAGTCACTTTTATCTGACTCCTTCTCATCTTCCTCTAGCATGATGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

355

Amino Acids

41.22

Weight (kDa)

5.6

Isoelectric Point (pI)

73.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 188 - 235 1.5e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 717
AciI CCGC 4 cut(s) 152, 493, 850, 926
AclWI GGATC 2 cut(s) 56, 69
AcsI RAATTY 1 cut(s) 146
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 3 cut(s) 129, 535, 587
AfiI CCNNNNNNNGG 1 cut(s) 717
AflIII ACRYGT 1 cut(s) 463
AgsI TTSAA 2 cut(s) 227, 646
AjnI CCWGG 1 cut(s) 247
AjuI GAANNNNNNNTTGG 2 cut(s) 79, 111
AluBI AGCT 4 cut(s) 543, 614, 766, 818
AluI AGCT 4 cut(s) 543, 614, 766, 818
Alw26I GTCTC 2 cut(s) 278, 850
AlwI GGATC 2 cut(s) 56, 69
Aor13HI TCCGGA 1 cut(s) 717
AoxI GGCC 2 cut(s) 680, 873
ApeKI GCWGC 6 cut(s) 373, 376, 379, 382, 406, 611
ApoI RAATTY 1 cut(s) 146
Asp700I GAANNNNTTC 1 cut(s) 544
AspLEI GCGC 1 cut(s) 333
AsuHPI GGTGA 2 cut(s) 8, 688
BamHI GGATCC 1 cut(s) 61
BauI CACGAG 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 200
BbvI GCAGC 6 cut(s) 385, 388, 391, 394, 418, 623
BccI CCATC 3 cut(s) 27, 151, 802
BcgI CGANNNNNNTGC 2 cut(s) 955, 989
BciT130I CCWGG 1 cut(s) 249
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 2 cut(s) 278, 850
BfaI CTAG 4 cut(s) 429, 537, 567, 1053
BfoI RGCGCY 1 cut(s) 334
BisI GCNGC 6 cut(s) 374, 377, 380, 383, 407, 612
BlsI GCNGC 6 cut(s) 375, 378, 381, 384, 408, 613
BmcAI AGTACT 1 cut(s) 535
Bme1390I CCNGG 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 172
BpiI GAAGAC 1 cut(s) 200
BpmI CTGGAG 1 cut(s) 854
Bpu10I CCTNAGC 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 38
BsaWI WCCGGW 2 cut(s) 124, 717
BsaXI ACNNNNNCTCC 2 cut(s) 788, 818
Bsc4I CCNNNNNNNGG 1 cut(s) 717
Bse1I ACTGG 1 cut(s) 837
Bse3DI GCAATG 1 cut(s) 439
BseAI TCCGGA 1 cut(s) 717
BseBI CCWGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 909
BseLI CCNNNNNNNGG 1 cut(s) 717
BseMI GCAATG 1 cut(s) 439
BseMII CTCAG 2 cut(s) 279, 620
BseNI ACTGG 1 cut(s) 837
BseRI GAGGAG 5 cut(s) 571, 764, 1008, 1013, 1016
BseXI GCAGC 6 cut(s) 385, 388, 391, 394, 418, 623
BsgI GTGCAG 1 cut(s) 676
Bsh1285I CGRYCG 1 cut(s) 780
BshFI GGCC 2 cut(s) 682, 875
BsiEI CGRYCG 1 cut(s) 780
BsiSI CCGG 2 cut(s) 125, 718
BslI CCNNNNNNNGG 1 cut(s) 717
BsmAI GTCTC 2 cut(s) 278, 850
BsmI GAATGC 2 cut(s) 778, 928
BsnI GGCC 2 cut(s) 682, 875
Bsp13I TCCGGA 1 cut(s) 717
Bsp143I GATC 5 cut(s) 61, 439, 508, 777, 894
BspACI CCGC 4 cut(s) 152, 493, 850, 926
BspANI GGCC 2 cut(s) 682, 875
BspCNI CTCAG 2 cut(s) 280, 619
BspEI TCCGGA 1 cut(s) 717
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 1 cut(s) 63
BspPI GGATC 2 cut(s) 56, 69
BspQI GCTCTTC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 439
BsrI ACTGG 1 cut(s) 837
BssMI GATC 5 cut(s) 61, 439, 508, 777, 894
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 957
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 9 cut(s) 5, 192, 468, 476, 482, 741, 753, 881, 1005
BstACI GRCGYC 1 cut(s) 38
BstDEI CTNAG 4 cut(s) 57, 288, 606, 615
BstF5I GGATG 1 cut(s) 909
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 5 cut(s) 64, 442, 511, 780, 897
BstMAI GTCTC 2 cut(s) 278, 850
BstMBI GATC 5 cut(s) 61, 439, 508, 777, 894
BstMCI CGRYCG 1 cut(s) 780
BstMWI GCNNNNNNNGC 4 cut(s) 160, 379, 382, 388
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 2 cut(s) 467, 598
BstSCI CCNGG 1 cut(s) 247
BstV1I GCAGC 6 cut(s) 385, 388, 391, 394, 418, 623
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 2 cut(s) 682, 875
BtsCI GGATG 1 cut(s) 909
BtsIMutI CAGTG 2 cut(s) 118, 310
CciI TCATGA 1 cut(s) 511
CfoI GCGC 1 cut(s) 333
CseI GACGC 1 cut(s) 46
Csp6I GTAC 3 cut(s) 128, 534, 586
CviAII CATG 7 cut(s) 196, 464, 499, 512, 595, 898, 1057
CviQI GTAC 3 cut(s) 128, 534, 586
DdeI CTNAG 4 cut(s) 57, 288, 606, 615
DpnI GATC 5 cut(s) 63, 441, 510, 779, 896
DpnII GATC 5 cut(s) 61, 439, 508, 777, 894
Eam1104I CTCTTC 9 cut(s) 5, 192, 468, 476, 482, 741, 753, 881, 1005
EarI CTCTTC 9 cut(s) 5, 192, 468, 476, 482, 741, 753, 881, 1005
EciI GGCGGA 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 247
FaeI CATG 7 cut(s) 199, 467, 502, 515, 598, 901, 1060
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FatI CATG 7 cut(s) 195, 463, 498, 511, 594, 897, 1056
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 6 cut(s) 374, 377, 380, 383, 407, 612
FokI GGATG 1 cut(s) 916
Fsp4HI GCNGC 6 cut(s) 374, 377, 380, 383, 407, 612
FspBI CTAG 4 cut(s) 429, 537, 567, 1053
GlaI GCGC 1 cut(s) 332
GluI GCNGC 6 cut(s) 374, 377, 380, 383, 407, 612
GsuI CTGGAG 1 cut(s) 854
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 2 cut(s) 682, 875
HapII CCGG 2 cut(s) 125, 718
HgaI GACGC 1 cut(s) 46
HhaI GCGC 1 cut(s) 333
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 7 cut(s) 199, 467, 502, 515, 598, 901, 1060
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 4 cut(s) 13, 25, 907, 1034
HpaI GTTAAC 1 cut(s) 301
HpaII CCGG 2 cut(s) 125, 718
HphI GGTGA 2 cut(s) 8, 688
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 6 cut(s) 24, 236, 690, 771, 912, 1033
Hpy188III TCNNGA 7 cut(s) 65, 512, 555, 718, 728, 821, 983
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 3 cut(s) 556, 678, 977
HpyAV CCTTC 2 cut(s) 222, 1048
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4V TGCA 4 cut(s) 163, 498, 657, 829
HpyF10VI GCNNNNNNNGC 4 cut(s) 160, 379, 382, 388
HpyF3I CTNAG 4 cut(s) 57, 288, 606, 615
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 7 cut(s) 199, 467, 502, 515, 598, 901, 1060
HspAI GCGC 1 cut(s) 331
Kpn2I TCCGGA 1 cut(s) 717
Ksp22I TGATCA 1 cut(s) 508
KspAI GTTAAC 1 cut(s) 301
Kzo9I GATC 5 cut(s) 61, 439, 508, 777, 894
LguI GCTCTTC 1 cut(s) 192
LmnI GCTCC 2 cut(s) 127, 823
Lsp1109I GCAGC 6 cut(s) 385, 388, 391, 394, 418, 623
LweI GCATC 1 cut(s) 172
MaeI CTAG 4 cut(s) 429, 537, 567, 1053
MaeIII GTNAC 2 cut(s) 14, 1020
MalI GATC 5 cut(s) 63, 441, 510, 779, 896
MboI GATC 5 cut(s) 61, 439, 508, 777, 894
MflI RGATCY 1 cut(s) 61
MluCI AATT 6 cut(s) 43, 92, 146, 415, 504, 937
MlyI GAGTC 3 cut(s) 22, 34, 1028
MmeI TCCRAC 2 cut(s) 259, 768
MroI TCCGGA 1 cut(s) 717
MroXI GAANNNNTTC 1 cut(s) 544
MseI TTAA 3 cut(s) 300, 870, 1017
MslI CAYNNNNRTG 1 cut(s) 902
MspI CCGG 2 cut(s) 125, 718
MspR9I CCNGG 1 cut(s) 249
Mva1269I GAATGC 2 cut(s) 778, 928
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 4 cut(s) 160, 379, 382, 388
NdeII GATC 5 cut(s) 61, 439, 508, 777, 894
NlaIII CATG 7 cut(s) 199, 467, 502, 515, 598, 901, 1060
NlaIV GGNNCC 1 cut(s) 63
NmuCI GTSAC 2 cut(s) 14, 1020
NspI RCATGY 2 cut(s) 467, 598
PagI TCATGA 1 cut(s) 511
PciI ACATGT 1 cut(s) 463
PciSI GCTCTTC 1 cut(s) 192
PctI GAATGC 2 cut(s) 778, 928
PdmI GAANNNNTTC 1 cut(s) 544
PfeI GAWTC 1 cut(s) 907
PfoI TCCNGGA 1 cut(s) 247
PkrI GCNGC 6 cut(s) 375, 378, 381, 384, 408, 613
Ple19I CGATCG 1 cut(s) 780
PleI GAGTC 3 cut(s) 21, 33, 1028
PpsI GAGTC 3 cut(s) 21, 33, 1028
PscI ACATGT 1 cut(s) 463
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 63
PsuI RGATCY 1 cut(s) 61
PvuI CGATCG 1 cut(s) 780
RsaI GTAC 3 cut(s) 129, 535, 587
RsaNI GTAC 3 cut(s) 128, 534, 586
RseI CAYNNNNRTG 1 cut(s) 902
SapI GCTCTTC 1 cut(s) 192
SaqAI TTAA 3 cut(s) 300, 870, 1017
SatI GCNGC 6 cut(s) 374, 377, 380, 383, 407, 612
Sau3AI GATC 5 cut(s) 61, 439, 508, 777, 894
ScaI AGTACT 1 cut(s) 535
SchI GAGTC 3 cut(s) 22, 34, 1028
ScrFI CCNGG 1 cut(s) 249
SetI ASST 8 cut(s) 232, 243, 415, 545, 616, 656, 768, 820
SfaNI GCATC 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 902
Sse9I AATT 6 cut(s) 43, 92, 146, 415, 504, 937
SsiI CCGC 4 cut(s) 152, 493, 850, 926
SspMI CTAG 4 cut(s) 429, 537, 567, 1053
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 1 cut(s) 305
TaqI TCGA 4 cut(s) 219, 554, 780, 991
TasI AATT 6 cut(s) 43, 92, 146, 415, 504, 937
TatI WGTACW 1 cut(s) 533
TfiI GAWTC 1 cut(s) 907
Tru1I TTAA 3 cut(s) 300, 870, 1017
Tru9I TTAA 3 cut(s) 300, 870, 1017
TscAI CASTG 2 cut(s) 118, 310
TseFI GTSAC 2 cut(s) 14, 1020
TseI GCWGC 6 cut(s) 373, 376, 379, 382, 406, 611
Tsp45I GTSAC 2 cut(s) 14, 1020
TspDTI ATGAA 7 cut(s) 23, 159, 184, 450, 528, 537, 920
TspGWI ACGGA 1 cut(s) 961
TspRI CASTG 2 cut(s) 118, 310
XapI RAATTY 1 cut(s) 146
XceI RCATGY 2 cut(s) 467, 598
XmnI GAANNNNTTC 1 cut(s) 544
XspI CTAG 4 cut(s) 429, 537, 567, 1053
ZrmI AGTACT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.