MD06G1193600.v1.1

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012997
Physical Location & Seq
Forward (+)
32879681 .. 32881256
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1193600.v1.1.491

Sequence Viewer

Length: 984 bp
ATGTATGGGATTTTCATCCATTTGATATATATACCCACTCGTCTCTTCTCCTTCTTCCTCCTCCTCCTGCTGGGGTATGCTGCATTGGATTGGCAATTTGATTTGCCAATCGTACAAGATCATAGCTTTCTTGATCCTCTTCCACTCGAGAGCTGCGCGGATATTGATCCTATTTACTCCTCACTCGACATTCCACCAATCCGAACTGTATTAGAAGATGATGGAATTTTCTTTGCAAACGAAACGGTCTTGTTGGGAGTTAATTCTAGTAATCTCTGCGCTGGGTTAACTGAGGTTAATCACAATCATCATCAGCAATATCAACAACAACAACAGCAACCTTTGCTTCCGACATGCGAAAATGAAGAAAATGGCATAACTGACGAGAACGAGTCAAGGGAAGAGAGAAATAAGGTGATTAGTAAGAGGCCACACTCTTATAAAAGACATAGATCATCAAGCTCCTCTCTCTCTTCCTCCAAAATGTTGTCAAGAGAGACCGTTTCGCAGTACTTTTACATGCCAATCACTCAGGCAGCAAAAGAACTCAACGTAGGGCTAACCCTTCTCAAAAAAAGGTGTCGGGAGTTAGGCATTCGTCGGTGGCCTCACCGGAAGCTGAACAGCCTTCAAACCCTAATCCGAAACATTCAGGAGTTGGGAAAGGAAGGAGAAGAGAGCTCAGAGAAGCTTCGGAATGCGATCACGTTGCTGGAGAGGGAGAGGAAGCTGCTGGAGGAAGTGCCTGACATGCAGCTTGAAGACAATACGAAGCGTTTGAGGCAGGCCTGCTTCAAAGCCAATTACAAGAAGAGGAAGATCATGGGGATGAATGTTATGGGACAATCGCTATCATCTTTCAGTTGCAGCAACATTCAGTATCCAACAAGCTCGATGGATGCTGACGATGAAGACGAAGAAATTAAGTCTCTCTTGTCCGACTCTTTTTCCTCAAATAACAATGCATGCAACACTCTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

328

Amino Acids

37.62

Weight (kDa)

5.7

Isoelectric Point (pI)

62.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 163 - 210 2.8e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 441
AccII CGCG 1 cut(s) 158
AciI CCGC 1 cut(s) 158
AclWI GGATC 2 cut(s) 128, 161
AcsI RAATTY 1 cut(s) 225
AfaI GTAC 2 cut(s) 114, 512
AfiI CCNNNNNNNGG 1 cut(s) 70
AgsI TTSAA 3 cut(s) 632, 761, 796
AluBI AGCT 9 cut(s) 126, 153, 462, 619, 681, 691, 730, 757, 891
AluI AGCT 9 cut(s) 126, 153, 462, 619, 681, 691, 730, 757, 891
Alw21I GWGCWC 1 cut(s) 683
Alw26I GTCTC 3 cut(s) 47, 491, 933
AlwI GGATC 2 cut(s) 128, 161
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 3 cut(s) 428, 605, 786
ApeKI GCWGC 6 cut(s) 80, 153, 536, 730, 754, 867
ApoI RAATTY 1 cut(s) 225
AspLEI GCGC 2 cut(s) 158, 281
AsuHPI GGTGA 2 cut(s) 427, 602
AvaI CYCGRG 1 cut(s) 146
BanII GRGCYC 1 cut(s) 683
BbsI GAAGAC 2 cut(s) 768, 918
Bbv12I GWGCWC 1 cut(s) 683
BbvI GCAGC 6 cut(s) 67, 140, 548, 717, 766, 879
BccI CCATC 2 cut(s) 215, 889
BcgI CGANNNNNNTGC 2 cut(s) 691, 725
BciVI GTATCC 1 cut(s) 891
BcoDI GTCTC 3 cut(s) 47, 491, 933
BfaI CTAG 1 cut(s) 267
BfuI GTATCC 1 cut(s) 891
BisI GCNGC 6 cut(s) 81, 154, 537, 731, 755, 868
BlsI GCNGC 6 cut(s) 82, 155, 538, 732, 756, 869
BmcAI AGTACT 1 cut(s) 512
BmeT110I CYCGRG 1 cut(s) 146
BmsI GCATC 1 cut(s) 889
BpiI GAAGAC 2 cut(s) 768, 918
BpmI CTGGAG 2 cut(s) 734, 755
BsaBI GATNNNNATC 2 cut(s) 14, 165
BsaI GGTCTC 1 cut(s) 491
BsaWI WCCGGW 1 cut(s) 612
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse8I GATNNNNATC 2 cut(s) 14, 165
BseGI GGATG 3 cut(s) 15, 834, 904
BseJI GATNNNNATC 2 cut(s) 14, 165
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMII CTCAG 3 cut(s) 282, 545, 696
BseRI GAGGAG 4 cut(s) 50, 53, 169, 454
BseXI GCAGC 6 cut(s) 67, 140, 548, 717, 766, 879
BseYI CCCAGC 2 cut(s) 70, 281
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 3 cut(s) 430, 607, 788
BsiHKAI GWGCWC 1 cut(s) 683
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 1 cut(s) 613
BslFI GGGAC 1 cut(s) 855
BslI CCNNNNNNNGG 1 cut(s) 70
BsmAI GTCTC 3 cut(s) 47, 491, 933
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 855
BsmI GAATGC 2 cut(s) 594, 703
BsnI GGCC 3 cut(s) 430, 607, 788
Bso31I GGTCTC 1 cut(s) 491
BsoBI CYCGRG 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 683
Bsp143I GATC 6 cut(s) 118, 133, 166, 452, 702, 819
BspACI CCGC 1 cut(s) 158
BspANI GGCC 3 cut(s) 430, 607, 788
BspCNI CTCAG 3 cut(s) 283, 544, 695
BspFNI CGCG 1 cut(s) 158
BspPI GGATC 2 cut(s) 128, 161
BspTNI GGTCTC 1 cut(s) 491
BssMI GATC 6 cut(s) 118, 133, 166, 452, 702, 819
Bst4CI ACNGT 3 cut(s) 208, 247, 502
Bst6I CTCTTC 6 cut(s) 50, 144, 396, 478, 669, 806
BstAPI GCANNNNNTGC 1 cut(s) 343
BstC8I GCNNGC 3 cut(s) 786, 790, 967
BstDEI CTNAG 3 cut(s) 291, 531, 682
BstF5I GGATG 3 cut(s) 15, 834, 904
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 2 cut(s) 158, 281
BstKTI GATC 6 cut(s) 121, 136, 169, 455, 705, 822
BstMAI GTCTC 3 cut(s) 47, 491, 933
BstMBI GATC 6 cut(s) 118, 133, 166, 452, 702, 819
BstMWI GCNNNNNNNGC 3 cut(s) 343, 751, 781
BstNSI RCATGY 4 cut(s) 357, 523, 754, 969
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 6 cut(s) 67, 140, 548, 717, 766, 879
BstV2I GAAGAC 2 cut(s) 768, 918
BsuI GTATCC 1 cut(s) 891
BsuRI GGCC 3 cut(s) 430, 607, 788
BtsCI GGATG 3 cut(s) 15, 834, 904
Cac8I GCNNGC 3 cut(s) 786, 790, 967
CfoI GCGC 2 cut(s) 158, 281
Csp6I GTAC 2 cut(s) 113, 511
CviAII CATG 5 cut(s) 354, 520, 751, 823, 966
CviQI GTAC 2 cut(s) 113, 511
DdeI CTNAG 3 cut(s) 291, 531, 682
DpnI GATC 6 cut(s) 120, 135, 168, 454, 704, 821
DpnII GATC 6 cut(s) 118, 133, 166, 452, 702, 819
Eam1104I CTCTTC 6 cut(s) 50, 144, 396, 478, 669, 806
EarI CTCTTC 6 cut(s) 50, 144, 396, 478, 669, 806
Ecl136II GAGCTC 1 cut(s) 681
Eco147I AGGCCT 1 cut(s) 788
Eco24I GRGCYC 1 cut(s) 683
Eco31I GGTCTC 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 681
Eco88I CYCGRG 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 681
EcoT22I ATGCAT 1 cut(s) 967
EcoT38I GRGCYC 1 cut(s) 683
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 5 cut(s) 357, 523, 754, 826, 969
FalI AAGNNNNNCTT 2 cut(s) 917, 949
FaqI GGGAC 1 cut(s) 855
FatI CATG 5 cut(s) 353, 519, 750, 822, 965
Fnu4HI GCNGC 6 cut(s) 81, 154, 537, 731, 755, 868
FokI GGATG 3 cut(s) 2, 841, 911
FriOI GRGCYC 1 cut(s) 683
Fsp4HI GCNGC 6 cut(s) 81, 154, 537, 731, 755, 868
FspBI CTAG 1 cut(s) 267
GlaI GCGC 2 cut(s) 157, 280
GluI GCNGC 6 cut(s) 81, 154, 537, 731, 755, 868
GsaI CCCAGC 2 cut(s) 74, 285
GsuI CTGGAG 2 cut(s) 734, 755
HaeIII GGCC 3 cut(s) 430, 607, 788
HapII CCGG 1 cut(s) 613
HhaI GCGC 2 cut(s) 158, 281
Hin1II CATG 5 cut(s) 357, 523, 754, 826, 969
Hin6I GCGC 2 cut(s) 156, 279
HinP1I GCGC 2 cut(s) 156, 279
HincII GTYRAC 1 cut(s) 288
HindII GTYRAC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 2 cut(s) 392, 941
HpaI GTTAAC 1 cut(s) 288
HpaII CCGG 1 cut(s) 613
HphI GGTGA 2 cut(s) 427, 602
Hpy166II GTNNAC 1 cut(s) 288
Hpy188I TCNGA 6 cut(s) 203, 351, 644, 685, 696, 940
Hpy188III TCNNGA 5 cut(s) 131, 148, 492, 584, 653
Hpy8I GTNNAC 1 cut(s) 288
Hpy99I CGWCG 1 cut(s) 603
HpyAV CCTTC 4 cut(s) 61, 575, 638, 662
HpyCH4III ACNGT 3 cut(s) 208, 247, 502
HpyCH4IV ACGT 2 cut(s) 552, 707
HpyCH4V TGCA 6 cut(s) 83, 236, 754, 867, 965, 969
HpyF10VI GCNNNNNNNGC 3 cut(s) 343, 751, 781
HpyF3I CTNAG 3 cut(s) 291, 531, 682
HpySE526I ACGT 2 cut(s) 552, 707
Hsp92II CATG 5 cut(s) 357, 523, 754, 826, 969
HspAI GCGC 2 cut(s) 156, 279
KspAI GTTAAC 1 cut(s) 288
Kzo9I GATC 6 cut(s) 118, 133, 166, 452, 702, 819
LmnI GCTCC 1 cut(s) 467
Lsp1109I GCAGC 6 cut(s) 67, 140, 548, 717, 766, 879
LweI GCATC 1 cut(s) 889
MaeI CTAG 1 cut(s) 267
MaeII ACGT 2 cut(s) 552, 707
MalI GATC 6 cut(s) 120, 135, 168, 454, 704, 821
MboI GATC 6 cut(s) 118, 133, 166, 452, 702, 819
MhlI GDGCHC 1 cut(s) 683
MluCI AATT 5 cut(s) 95, 225, 262, 802, 921
MlyI GAGTC 2 cut(s) 401, 935
MmeI TCCRAC 3 cut(s) 374, 908, 963
Mph1103I ATGCAT 1 cut(s) 967
MseI TTAA 5 cut(s) 261, 287, 297, 924, 982
MslI CAYNNNNRTG 1 cut(s) 827
MspI CCGG 1 cut(s) 613
Mva1269I GAATGC 2 cut(s) 594, 703
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 3 cut(s) 343, 751, 781
NdeII GATC 6 cut(s) 118, 133, 166, 452, 702, 819
NlaIII CATG 5 cut(s) 357, 523, 754, 826, 969
NsiI ATGCAT 1 cut(s) 967
NspI RCATGY 4 cut(s) 357, 523, 754, 969
PaeI GCATGC 1 cut(s) 969
PaeR7I CTCGAG 1 cut(s) 146
PceI AGGCCT 1 cut(s) 788
PcsI WCGNNNNNNNCGW 1 cut(s) 912
PctI GAATGC 2 cut(s) 594, 703
PkrI GCNGC 6 cut(s) 82, 155, 538, 732, 756, 869
PleI GAGTC 2 cut(s) 400, 935
PpsI GAGTC 2 cut(s) 400, 935
PsiI TTATAA 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 683
PspFI CCCAGC 2 cut(s) 70, 281
RsaI GTAC 2 cut(s) 114, 512
RsaNI GTAC 2 cut(s) 113, 511
RseI CAYNNNNRTG 1 cut(s) 827
SacI GAGCTC 1 cut(s) 683
SaqAI TTAA 5 cut(s) 261, 287, 297, 924, 982
SatI GCNGC 6 cut(s) 81, 154, 537, 731, 755, 868
Sau3AI GATC 6 cut(s) 118, 133, 166, 452, 702, 819
ScaI AGTACT 1 cut(s) 512
SchI GAGTC 2 cut(s) 401, 935
SduI GDGCHC 1 cut(s) 683
SfaNI GCATC 1 cut(s) 889
Sfr274I CTCGAG 1 cut(s) 146
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 827
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SphI GCATGC 1 cut(s) 969
Sse9I AATT 5 cut(s) 95, 225, 262, 802, 921
SseBI AGGCCT 1 cut(s) 788
SsiI CCGC 1 cut(s) 158
SspMI CTAG 1 cut(s) 267
SstI GAGCTC 1 cut(s) 683
StuI AGGCCT 1 cut(s) 788
TaaI ACNGT 3 cut(s) 208, 247, 502
TaiI ACGT 2 cut(s) 555, 710
TaqI TCGA 3 cut(s) 147, 186, 893
TasI AATT 5 cut(s) 95, 225, 262, 802, 921
TatI WGTACW 1 cut(s) 510
Tru1I TTAA 5 cut(s) 261, 287, 297, 924, 982
Tru9I TTAA 5 cut(s) 261, 287, 297, 924, 982
TseI GCWGC 6 cut(s) 80, 153, 536, 730, 754, 867
TspDTI ATGAA 4 cut(s) 4, 378, 845, 924
XapI RAATTY 1 cut(s) 225
XceI RCATGY 4 cut(s) 357, 523, 754, 969
XhoI CTCGAG 1 cut(s) 146
XspI CTAG 1 cut(s) 267
ZrmI AGTACT 1 cut(s) 512
Zsp2I ATGCAT 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.