pycom14g16650

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012997
Physical Location & Seq
Forward (+)
18866109 .. 18867422
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g16650.2

Sequence Viewer

Length: 1077 bp
ATGGGTTGGCTGGCCAAGTACGACGTCGTCAATGATCTTGGTGAAGACCCATATTTCTTCCCACATCAACCTTCATTTCCCTCTGTACATGATTTCCGGTTAAATTATATTAGGGGGTATGCATGCGATTGGCAATTTGATTTGCCAATAGTACAAGATCATAGATTTCCTGATCCTCTTCCTTCACTTGAGAGCTGTGTGAATATTGATCCCATTTACTCCTCACTTGACATTCCACCAATCCGAACTGTATTGCAAGGTGTGTTTCAGTATTATGATGATGAAATTTTCTTCGGAAATGGAAACGCGTTGTTTGGAGTTAATTCTAATGATTTATGTGGTGGGTTAACTGAGATTAATCACAATCATCATCAGCAATTTCAACAACAGCAGCAACCTCCATTGCTTCTGACTTGCAAGAACGATGAAAATGGCATTACGGACATGAACGATTCCCTGGAAGAGAGGGATAAGGCGATTAGTGAGAGGCCACACTCTTCTAAAAGACATAGATCATCAAGCTCTTCCCTCTCTTCCTCCAAAATGTTGTCAAGACAGACCATTTCCCAGTACTTCTACATGCCAATCACTCAGGCTGCCAAGGAACTCAACGTAGGGCTAACTCTTCTGAAGAAAAGGTGTCGAGAGTTGGGCATTCGTCGTTGGCCTCATCGGAAGTTGACCAGCCTTCAAACCCTAATCCGAAACATTCAGGTTAATAGGCGTTACATATTTGTACGTGTTCGTGAGTTAGGAAAGGAAGGAGAGGAGGGCGAAGAGAAGCTTCGAAACGCGATCGCGTTGCTGGAGATGGAGAGGAAGCTGCTGGAGGAAGCGCCTGATATGCAGCTTGAGGACAATACGAAGCGTCTAAGGCAGGCATGCTTCAAAGCAAATTACAAGAAGAGGAAGATCATGGGGACGAAGGTTATGGAAAAATCACAATCATCTTTTAGTTGCAGCAACATTCAGTATCCAACAAGTTCGATGGATGCTAACGAGGAAGACGAAGAAATCAAGTCTCTATTGTCTGACTCTTTTTCCTCGAATAACAATGCATGCATCACTCTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

359

Amino Acids

41.39

Weight (kDa)

6.18

Isoelectric Point (pI)

65.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 27
AccII CGCG 3 cut(s) 308, 794, 800
AclWI GGATC 2 cut(s) 167, 203
AcoI YGGCCR 1 cut(s) 12
AcsI RAATTY 1 cut(s) 285
AcuI CTGAAG 1 cut(s) 650
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 5 cut(s) 20, 87, 153, 572, 738
AflIII ACRYGT 2 cut(s) 306, 739
AgsI TTSAA 3 cut(s) 383, 692, 889
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 5 cut(s) 195, 522, 784, 823, 850
AluI AGCT 5 cut(s) 195, 522, 784, 823, 850
Alw26I GTCTC 1 cut(s) 1026
AlwI GGATC 2 cut(s) 167, 203
AoxI GGCC 3 cut(s) 12, 488, 665
ApeKI GCWGC 5 cut(s) 391, 596, 823, 847, 960
ApoI RAATTY 1 cut(s) 285
AseI ATTAAT 1 cut(s) 357
AsiSI GCGATCGC 1 cut(s) 798
AspLEI GCGC 1 cut(s) 838
AsuHPI GGTGA 1 cut(s) 53
AsuII TTCGAA 1 cut(s) 787
BalI TGGCCA 1 cut(s) 14
BbsI GAAGAC 2 cut(s) 51, 1011
BbvI GCAGC 5 cut(s) 403, 583, 810, 859, 972
BccI CCATC 2 cut(s) 805, 982
BcgI CGANNNNNNTGC 2 cut(s) 784, 818
BciT130I CCWGG 1 cut(s) 458
BciVI GTATCC 1 cut(s) 984
BcoDI GTCTC 1 cut(s) 1026
BfoI RGCGCY 1 cut(s) 839
BfuI GTATCC 1 cut(s) 984
BisI GCNGC 5 cut(s) 392, 597, 824, 848, 961
BlsI GCNGC 5 cut(s) 393, 598, 825, 849, 962
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 1 cut(s) 458
BmrFI CCNGG 1 cut(s) 458
BmrI ACTGGG 1 cut(s) 562
BmsI GCATC 2 cut(s) 982, 1071
BmuI ACTGGG 1 cut(s) 562
BpiI GAAGAC 2 cut(s) 51, 1011
BpmI CTGGAG 2 cut(s) 827, 848
Bpu14I TTCGAA 1 cut(s) 787
BpuEI CTTGAG 2 cut(s) 209, 872
BsaAI YACGTR 1 cut(s) 740
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 456, 600
BsaWI WCCGGW 1 cut(s) 96
Bse1I ACTGG 1 cut(s) 568
Bse3DI GCAATG 1 cut(s) 401
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 2 cut(s) 456, 600
BseGI GGATG 1 cut(s) 997
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 2 cut(s) 342, 605
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 2 cut(s) 211, 782
BseXI GCAGC 5 cut(s) 403, 583, 810, 859, 972
Bsh1236I CGCG 3 cut(s) 308, 794, 800
Bsh1285I CGRYCG 1 cut(s) 798
BshFI GGCC 3 cut(s) 14, 490, 667
BsiEI CGRYCG 1 cut(s) 798
BsiSI CCGG 1 cut(s) 97
BslFI GGGAC 1 cut(s) 934
BsmAI GTCTC 1 cut(s) 1026
BsmFI GGGAC 1 cut(s) 934
BsmI GAATGC 1 cut(s) 654
BsnI GGCC 3 cut(s) 14, 490, 667
Bsp119I TTCGAA 1 cut(s) 787
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
BspANI GGCC 3 cut(s) 14, 490, 667
BspCNI CTCAG 2 cut(s) 343, 604
BspFNI CGCG 3 cut(s) 308, 794, 800
BspPI GGATC 2 cut(s) 167, 203
BspQI GCTCTTC 1 cut(s) 529
BspT104I TTCGAA 1 cut(s) 787
BsrDI GCAATG 1 cut(s) 401
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 1 cut(s) 568
BssECI CCNNGG 2 cut(s) 456, 600
BssMI GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 600
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 8 cut(s) 183, 456, 502, 529, 538, 630, 771, 899
BstACI GRCGYC 1 cut(s) 24
BstAUI TGTACA 1 cut(s) 85
BstBAI YACGTR 1 cut(s) 740
BstBI TTCGAA 1 cut(s) 787
BstC8I GCNNGC 5 cut(s) 12, 124, 879, 883, 1060
BstDEI CTNAG 3 cut(s) 351, 591, 872
BstF5I GGATG 1 cut(s) 997
BstFNI CGCG 3 cut(s) 308, 794, 800
BstH2I RGCGCY 1 cut(s) 839
BstHHI GCGC 1 cut(s) 838
BstKTI GATC 7 cut(s) 37, 160, 175, 211, 515, 798, 915
BstMAI GTCTC 1 cut(s) 1026
BstMBI GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
BstMCI CGRYCG 1 cut(s) 798
BstMWI GCNNNNNNNGC 2 cut(s) 844, 874
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 4 cut(s) 126, 583, 885, 1062
BstSCI CCNGG 1 cut(s) 456
BstUI CGCG 3 cut(s) 308, 794, 800
BstV1I GCAGC 5 cut(s) 403, 583, 810, 859, 972
BstV2I GAAGAC 2 cut(s) 51, 1011
BsuI GTATCC 1 cut(s) 984
BsuRI GGCC 3 cut(s) 14, 490, 667
BtsCI GGATG 1 cut(s) 997
Cac8I GCNNGC 5 cut(s) 12, 124, 879, 883, 1060
CfoI GCGC 1 cut(s) 838
CseI GACGC 1 cut(s) 857
Csp6I GTAC 5 cut(s) 19, 86, 152, 571, 737
CviAII CATG 7 cut(s) 89, 123, 445, 580, 882, 916, 1059
CviQI GTAC 5 cut(s) 19, 86, 152, 571, 737
DdeI CTNAG 3 cut(s) 351, 591, 872
DpnI GATC 7 cut(s) 36, 159, 174, 210, 514, 797, 914
DpnII GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 8 cut(s) 183, 456, 502, 529, 538, 630, 771, 899
EarI CTCTTC 8 cut(s) 183, 456, 502, 529, 538, 630, 771, 899
Eco130I CCWWGG 1 cut(s) 600
Eco57I CTGAAG 1 cut(s) 650
EcoRII CCWGG 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 600
EcoT22I ATGCAT 3 cut(s) 124, 1060, 1064
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 7 cut(s) 92, 126, 448, 583, 885, 919, 1062
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FaqI GGGAC 1 cut(s) 934
FatI CATG 7 cut(s) 88, 122, 444, 579, 881, 915, 1058
Fnu4HI GCNGC 5 cut(s) 392, 597, 824, 848, 961
FokI GGATG 1 cut(s) 1004
Fsp4HI GCNGC 5 cut(s) 392, 597, 824, 848, 961
GlaI GCGC 1 cut(s) 837
GluI GCNGC 5 cut(s) 392, 597, 824, 848, 961
GsuI CTGGAG 2 cut(s) 827, 848
HaeII RGCGCY 1 cut(s) 839
HaeIII GGCC 3 cut(s) 14, 490, 667
HapII CCGG 1 cut(s) 97
HgaI GACGC 1 cut(s) 857
HhaI GCGC 1 cut(s) 838
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 7 cut(s) 92, 126, 448, 583, 885, 919, 1062
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HincII GTYRAC 2 cut(s) 348, 681
HindII GTYRAC 2 cut(s) 348, 681
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 2 cut(s) 452, 1034
HpaI GTTAAC 1 cut(s) 348
HpaII CCGG 1 cut(s) 97
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 2 cut(s) 348, 681
Hpy188I TCNGA 7 cut(s) 245, 296, 411, 630, 675, 704, 1033
Hpy188III TCNNGA 4 cut(s) 170, 552, 644, 746
Hpy8I GTNNAC 2 cut(s) 348, 681
Hpy99I CGWCG 3 cut(s) 26, 29, 663
HpyAV CCTTC 5 cut(s) 81, 192, 698, 755, 919
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 3 cut(s) 24, 612, 739
HpyCH4V TGCA 7 cut(s) 122, 256, 417, 847, 960, 1058, 1062
HpyF10VI GCNNNNNNNGC 2 cut(s) 844, 874
HpyF3I CTNAG 3 cut(s) 351, 591, 872
HpySE526I ACGT 3 cut(s) 24, 612, 739
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 7 cut(s) 92, 126, 448, 583, 885, 919, 1062
HspAI GCGC 1 cut(s) 836
KspAI GTTAAC 1 cut(s) 348
Kzo9I GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
LguI GCTCTTC 1 cut(s) 529
Lsp1109I GCAGC 5 cut(s) 403, 583, 810, 859, 972
LweI GCATC 2 cut(s) 982, 1071
MaeII ACGT 3 cut(s) 24, 612, 739
MaeIII GTNAC 1 cut(s) 725
MalI GATC 7 cut(s) 36, 159, 174, 210, 514, 797, 914
MboI GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 6 cut(s) 103, 134, 285, 322, 377, 895
MluI ACGCGT 1 cut(s) 306
MluNI TGGCCA 1 cut(s) 14
MlyI GAGTC 1 cut(s) 1028
MmeI TCCRAC 1 cut(s) 1001
Mox20I TGGCCA 1 cut(s) 14
Mph1103I ATGCAT 3 cut(s) 124, 1060, 1064
MscI TGGCCA 1 cut(s) 14
MseI TTAA 6 cut(s) 101, 321, 347, 357, 717, 1075
Msp20I TGGCCA 1 cut(s) 14
MspI CCGG 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 458
Mva1269I GAATGC 1 cut(s) 654
MvaI CCWGG 1 cut(s) 458
MvnI CGCG 3 cut(s) 308, 794, 800
MwoI GCNNNNNNNGC 2 cut(s) 844, 874
NdeII GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
NlaIII CATG 7 cut(s) 92, 126, 448, 583, 885, 919, 1062
NsiI ATGCAT 3 cut(s) 124, 1060, 1064
NspI RCATGY 4 cut(s) 126, 583, 885, 1062
NspV TTCGAA 1 cut(s) 787
PaeI GCATGC 3 cut(s) 126, 885, 1062
PciSI GCTCTTC 1 cut(s) 529
PcsI WCGNNNNNNNCGW 1 cut(s) 1005
PctI GAATGC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 452
PflFI GACNNNGTC 1 cut(s) 26
PkrI GCNGC 5 cut(s) 393, 598, 825, 849, 962
Ple19I CGATCG 1 cut(s) 798
PleI GAGTC 1 cut(s) 1028
PpsI GAGTC 1 cut(s) 1028
Ppu21I YACGTR 1 cut(s) 740
PshBI ATTAAT 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PsyI GACNNNGTC 1 cut(s) 26
PvuI CGATCG 1 cut(s) 798
RgaI GCGATCGC 1 cut(s) 798
RsaI GTAC 5 cut(s) 20, 87, 153, 572, 738
RsaNI GTAC 5 cut(s) 19, 86, 152, 571, 737
SapI GCTCTTC 1 cut(s) 529
SaqAI TTAA 6 cut(s) 101, 321, 347, 357, 717, 1075
SatI GCNGC 5 cut(s) 392, 597, 824, 848, 961
Sau3AI GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
ScaI AGTACT 1 cut(s) 572
SchI GAGTC 1 cut(s) 1028
ScrFI CCNGG 1 cut(s) 458
SfaAI GCGATCGC 1 cut(s) 798
SfaNI GCATC 2 cut(s) 982, 1071
SfuI TTCGAA 1 cut(s) 787
SgfI GCGATCGC 1 cut(s) 798
SmlI CTYRAG 2 cut(s) 188, 851
SmoI CTYRAG 2 cut(s) 188, 851
SphI GCATGC 3 cut(s) 126, 885, 1062
Sse9I AATT 6 cut(s) 103, 134, 285, 322, 377, 895
SspI AATATT 1 cut(s) 205
StyD4I CCNGG 1 cut(s) 456
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 3 cut(s) 27, 615, 742
TaqI TCGA 4 cut(s) 643, 787, 986, 1046
TasI AATT 6 cut(s) 103, 134, 285, 322, 377, 895
TatI WGTACW 3 cut(s) 85, 151, 570
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 6 cut(s) 101, 321, 347, 357, 717, 1075
Tru9I TTAA 6 cut(s) 101, 321, 347, 357, 717, 1075
TseI GCWGC 5 cut(s) 391, 596, 823, 847, 960
TspDTI ATGAA 4 cut(s) 63, 297, 441, 461
TspGWI ACGGA 1 cut(s) 455
Tth111I GACNNNGTC 1 cut(s) 26
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 1 cut(s) 285
XceI RCATGY 4 cut(s) 126, 583, 885, 1062
ZraI GACGTC 1 cut(s) 25
ZrmI AGTACT 1 cut(s) 572
Zsp2I ATGCAT 3 cut(s) 124, 1060, 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.