Prupe.5G193900_v2.0.a1

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0012997
Physical Location & Seq
Forward (+)
15797961 .. 15799087
1127 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G193900.1

Sequence Viewer

Length: 912 bp
ATGAACGGGTATGGTCCATTTGATTGGCAATGCGAATGGACGCTCCCACAAGTACAAGACTCTTTTCTTGATGCTGCTGTTCCTTTCATGGATCAAAGCTGTTTGAATCTTGATCCTCTTTACTCTTCCCTTGATATTCAACCAATCCGAAGTGCGTTTCAAGGTGATGAAATTGTTTTGGGAAATGGAGATGGGGTTTGTAATGATTTGTTCTGTGAATTAATGGAGCCTAGCAATAATCAACAAAAACAAATACATCAACCTCCATTGCTGATGTGCAAGAATGAAGAAAATGGCAGTGCTGCGAATGAGTTGGAGGAGGAGACGAAGGTGATTAGTAAAAGGTCTCACCTCAGCACAAGGCTAAGATCATCAAACTCGACGTCGCTCAACTCATCAAAAATGTTGTCGAGGGAAACAATATCCAACTACTTCTACATGCCTATAACTCAGGCAGCCAAGGAACTCAATGTAGGTTTAACCCTTTTGAAGAAAAGGTGTAGAGAGTTGGGGATTCGCCGGTGGCCTCACCGGAAGCTGGCCAGCCTCCAAACCCTAATCAGAAACATTCAGGAGTTGGGAAAGGAGGGGGAGGAGAGTGAGGAGAAGCTTCGAAATGCAATACAGTTGTTGGAGAGGGAGAGGAAGCTGCTGGAGGAAGTGCCCGACATGCAATTGGAGGACAATACCAAGCGACTGAGGCAAGCTTGTTTTAAGGCTAATTACAAGAAGAGAAAGATCATGGGGATGAATAATATGGATCAATCACAATCCTCTTTCATCTGCAGCAACAATGAAATTCATGAATATGCAAGTATTATGGATGATGGTTATGAGGAAGATGAAGAAATCAAGTCTCTTTTATCTGACTCTTTTTCCTCATCAAATTCCAACAACATATGCCTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

304

Amino Acids

34.77

Weight (kDa)

5.05

Isoelectric Point (pI)

64.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 386
AclWI GGATC 3 cut(s) 99, 107, 768
AcoI YGGCCR 1 cut(s) 540
AcsI RAATTY 2 cut(s) 798, 886
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 538
AgsI TTSAA 4 cut(s) 106, 140, 161, 490
AluBI AGCT 5 cut(s) 99, 538, 610, 649, 707
AluI AGCT 5 cut(s) 99, 538, 610, 649, 707
Alw26I GTCTC 3 cut(s) 317, 351, 861
AlwI GGATC 3 cut(s) 99, 107, 768
AoxI GGCC 2 cut(s) 524, 540
ApeKI GCWGC 5 cut(s) 74, 302, 455, 649, 786
ApoI RAATTY 2 cut(s) 798, 886
ArsI GACNNNNNNTTYG 4 cut(s) 368, 392, 400, 424
AseI ATTAAT 1 cut(s) 221
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 4 cut(s) 176, 341, 343, 521
AsuII TTCGAA 1 cut(s) 613
AvaII GGWCC 1 cut(s) 14
BaeGI GKGCMC 1 cut(s) 666
BalI TGGCCA 1 cut(s) 542
BbvCI CCTCAGC 1 cut(s) 353
BbvI GCAGC 5 cut(s) 61, 289, 467, 636, 798
BccI CCATC 2 cut(s) 185, 821
BcoDI GTCTC 3 cut(s) 317, 351, 861
BfaI CTAG 2 cut(s) 231, 910
BfmI CTRYAG 1 cut(s) 784
BisI GCNGC 5 cut(s) 75, 303, 456, 650, 787
BlsI GCNGC 5 cut(s) 76, 304, 457, 651, 788
Bme18I GGWCC 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 228
BmsI GCATC 1 cut(s) 61
BpmI CTGGAG 1 cut(s) 674
Bpu10I CCTNAGC 1 cut(s) 353
Bpu14I TTCGAA 1 cut(s) 613
BsaHI GRCGYC 1 cut(s) 383
BsaI GGTCTC 1 cut(s) 351
BsaJI CCNNGG 1 cut(s) 459
BsaWI WCCGGW 1 cut(s) 531
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 538
Bse118I RCCGGY 1 cut(s) 519
Bse3DI GCAATG 2 cut(s) 35, 266
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 2 cut(s) 753, 829
BseLI CCNNNNNNNGG 1 cut(s) 538
BseMI GCAATG 2 cut(s) 35, 266
BseMII CTCAG 3 cut(s) 367, 464, 689
BseRI GAGGAG 4 cut(s) 332, 335, 608, 617
BseSI GKGCMC 1 cut(s) 666
BseXI GCAGC 5 cut(s) 61, 289, 467, 636, 798
BshFI GGCC 2 cut(s) 526, 542
BsiSI CCGG 2 cut(s) 520, 532
BslI CCNNNNNNNGG 1 cut(s) 538
BsmAI GTCTC 3 cut(s) 317, 351, 861
BsmBI CGTCTC 1 cut(s) 317
BsnI GGCC 2 cut(s) 526, 542
Bso31I GGTCTC 1 cut(s) 351
Bsp119I TTCGAA 1 cut(s) 613
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 5 cut(s) 91, 112, 368, 738, 760
BspANI GGCC 2 cut(s) 526, 542
BspCNI CTCAG 3 cut(s) 366, 463, 690
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 1 cut(s) 228
BspMAI CTGCAG 1 cut(s) 788
BspPI GGATC 3 cut(s) 99, 107, 768
BspT104I TTCGAA 1 cut(s) 613
BspTNI GGTCTC 1 cut(s) 351
BsrDI GCAATG 2 cut(s) 35, 266
BsrFI RCCGGY 1 cut(s) 519
BssAI RCCGGY 1 cut(s) 519
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 5 cut(s) 91, 112, 368, 738, 760
BssNI GRCGYC 1 cut(s) 383
BssT1I CCWWGG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 627
Bst6I CTCTTC 2 cut(s) 130, 725
BstACI GRCGYC 1 cut(s) 383
BstBI TTCGAA 1 cut(s) 613
BstC8I GCNNGC 3 cut(s) 540, 544, 705
BstDEI CTNAG 4 cut(s) 353, 365, 450, 698
BstF5I GGATG 2 cut(s) 753, 829
BstKTI GATC 5 cut(s) 94, 115, 371, 741, 763
BstMAI GTCTC 3 cut(s) 317, 351, 861
BstMBI GATC 5 cut(s) 91, 112, 368, 738, 760
BstMWI GCNNNNNNNGC 2 cut(s) 670, 700
BstNSI RCATGY 2 cut(s) 442, 673
BstSFI CTRYAG 1 cut(s) 784
BstSLI GKGCMC 1 cut(s) 666
BstV1I GCAGC 5 cut(s) 61, 289, 467, 636, 798
BstXI CCANNNNNNTGG 1 cut(s) 24
BsuRI GGCC 2 cut(s) 526, 542
BtsCI GGATG 2 cut(s) 753, 829
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 304
Cac8I GCNNGC 3 cut(s) 540, 544, 705
CciI TCATGA 1 cut(s) 802
Cfr10I RCCGGY 1 cut(s) 519
Cfr13I GGNCC 1 cut(s) 14
CseI GACGC 1 cut(s) 49
Csp6I GTAC 1 cut(s) 53
CviAII CATG 5 cut(s) 88, 439, 670, 742, 803
CviQI GTAC 1 cut(s) 53
DdeI CTNAG 4 cut(s) 353, 365, 450, 698
DpnI GATC 5 cut(s) 93, 114, 370, 740, 762
DpnII GATC 5 cut(s) 91, 112, 368, 738, 760
EaeI YGGCCR 1 cut(s) 540
Eam1104I CTCTTC 2 cut(s) 130, 725
EarI CTCTTC 2 cut(s) 130, 725
Eco130I CCWWGG 1 cut(s) 459
Eco31I GGTCTC 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 14
EcoT14I CCWWGG 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 317
FaeI CATG 5 cut(s) 91, 442, 673, 745, 806
FatI CATG 5 cut(s) 87, 438, 669, 741, 802
FauNDI CATATG 1 cut(s) 899
Fnu4HI GCNGC 5 cut(s) 75, 303, 456, 650, 787
FokI GGATG 2 cut(s) 760, 836
Fsp4HI GCNGC 5 cut(s) 75, 303, 456, 650, 787
FspBI CTAG 2 cut(s) 231, 910
GluI GCNGC 5 cut(s) 75, 303, 456, 650, 787
GsuI CTGGAG 1 cut(s) 674
HaeIII GGCC 2 cut(s) 526, 542
HapII CCGG 2 cut(s) 520, 532
HgaI GACGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 5 cut(s) 91, 442, 673, 745, 806
HindIII AAGCTT 2 cut(s) 608, 705
HinfI GANTC 4 cut(s) 59, 106, 514, 869
HpaII CCGG 2 cut(s) 520, 532
HphI GGTGA 4 cut(s) 176, 341, 343, 521
Hpy188I TCNGA 3 cut(s) 149, 563, 868
Hpy188III TCNNGA 4 cut(s) 68, 110, 572, 803
Hpy99I CGWCG 2 cut(s) 385, 388
HpyAV CCTTC 1 cut(s) 322
HpyCH4III ACNGT 1 cut(s) 627
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 5 cut(s) 279, 620, 673, 786, 812
HpyF10VI GCNNNNNNNGC 2 cut(s) 670, 700
HpyF3I CTNAG 4 cut(s) 353, 365, 450, 698
HpySE526I ACGT 1 cut(s) 383
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 5 cut(s) 91, 442, 673, 745, 806
Kzo9I GATC 5 cut(s) 91, 112, 368, 738, 760
LmnI GCTCC 2 cut(s) 48, 226
LpnPI CCDG 7 cut(s) 437, 524, 533, 545, 556, 557, 638
Lsp1109I GCAGC 5 cut(s) 61, 289, 467, 636, 798
LweI GCATC 1 cut(s) 61
MaeI CTAG 2 cut(s) 231, 910
MaeII ACGT 1 cut(s) 383
MalI GATC 5 cut(s) 93, 114, 370, 740, 762
MboI GATC 5 cut(s) 91, 112, 368, 738, 760
MboII GAAGA 6 cut(s) 117, 299, 502, 742, 851, 857
MfeI CAATTG 1 cut(s) 674
MhlI GDGCHC 1 cut(s) 666
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 6 cut(s) 171, 218, 674, 721, 798, 886
MluNI TGGCCA 1 cut(s) 542
MlyI GAGTC 2 cut(s) 53, 863
MmeI TCCRAC 3 cut(s) 294, 450, 612
Mox20I TGGCCA 1 cut(s) 542
MscI TGGCCA 1 cut(s) 542
MseI TTAA 3 cut(s) 221, 479, 714
MslI CAYNNNNRTG 2 cut(s) 746, 807
Msp20I TGGCCA 1 cut(s) 542
MspI CCGG 2 cut(s) 520, 532
MunI CAATTG 1 cut(s) 674
MwoI GCNNNNNNNGC 2 cut(s) 670, 700
NdeI CATATG 1 cut(s) 899
NdeII GATC 5 cut(s) 91, 112, 368, 738, 760
NlaIII CATG 5 cut(s) 91, 442, 673, 745, 806
NlaIV GGNNCC 1 cut(s) 228
NspI RCATGY 2 cut(s) 442, 673
NspV TTCGAA 1 cut(s) 613
PagI TCATGA 1 cut(s) 802
PfeI GAWTC 2 cut(s) 106, 514
PkrI GCNGC 5 cut(s) 76, 304, 457, 651, 788
PleI GAGTC 2 cut(s) 53, 863
PpsI GAGTC 2 cut(s) 53, 863
PshBI ATTAAT 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 1 cut(s) 14
PstI CTGCAG 1 cut(s) 788
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
RseI CAYNNNNRTG 2 cut(s) 746, 807
SaqAI TTAA 3 cut(s) 221, 479, 714
SatI GCNGC 5 cut(s) 75, 303, 456, 650, 787
Sau3AI GATC 5 cut(s) 91, 112, 368, 738, 760
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 2 cut(s) 53, 863
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 1 cut(s) 61
SfcI CTRYAG 1 cut(s) 784
SfuI TTCGAA 1 cut(s) 613
SgrAI CRCCGGYG 1 cut(s) 519
SinI GGWCC 1 cut(s) 14
SmiMI CAYNNNNRTG 2 cut(s) 746, 807
Sse9I AATT 6 cut(s) 171, 218, 674, 721, 798, 886
SspMI CTAG 2 cut(s) 231, 910
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 1 cut(s) 627
TaiI ACGT 1 cut(s) 386
TaqI TCGA 3 cut(s) 380, 410, 613
TasI AATT 6 cut(s) 171, 218, 674, 721, 798, 886
TatI WGTACW 1 cut(s) 52
TfiI GAWTC 2 cut(s) 106, 514
Tru1I TTAA 3 cut(s) 221, 479, 714
Tru9I TTAA 3 cut(s) 221, 479, 714
TscAI CASTG 1 cut(s) 304
TseI GCWGC 5 cut(s) 74, 302, 455, 649, 786
TspRI CASTG 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 14
VspI ATTAAT 1 cut(s) 221
XapI RAATTY 2 cut(s) 798, 886
XceI RCATGY 2 cut(s) 442, 673
XspI CTAG 2 cut(s) 231, 910
ZraI GACGTC 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.