Rh7BG042800

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012997
Physical Location & Seq
Forward (+)
2711866 .. 2713258
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG042800.1

Sequence Viewer

Length: 1083 bp
ATGACAATGAGGAGTACTCACCATCAGAGTCAATCCCTGACGAGTAACCCTAATTGGGTAGTTTCTAAGGATCATGAAAACCCATTCTCATTCCCAAATCAATTTTCTCCTGCTGATTTCAGTGGGTATGCTCCGGTACTTGATTGGGAAAATGAATTTCCGCCGATGGTGCAAGACTACACTTTTATTAATGATGCTCTTCCTTTCATGGAGAGTTGTCTTCCTTCGCTCGATATTCAACCAATCCGCCTATTTCCAGGAGATGATTTTTCCTTTGGAGATAATGGAAATGAGACTGAGAATGGAGTTAACAGTGAGTTGTTCGCTTCTGGGTTTGAGCGCCCTAATCATCATCATCAACAAGAACAAGAACAACAGCAACAGCAGCAGCAGCAACAACAACAACAAGAAAAGCAGCAACCTAATTTACTGCTTCCTAGCAATGAAGATCAAAGTGGTGCTGACAGAGAACATGTGGGTGAAGAGAGGGAGAGGAAGAGGAAGAGGCGGTGCATGAGAATTGATCATGAAGTGAAAAGTGGTAGTAGTACTAGAAGCTATTCATCGTCGAGATTATTGTCTAGGGAAACAATCTCCCCGTACTTTTACATGCCTATAACTCAGGCAGCGAAGGAACTGAATATAGGCTTGACCCTTTTGAAGAAAAGGTGCCGGGAGTTGGGTATTCGTCGTTGGCCTCATCGGAAACTCACCAGCCTCCAAACCCTAATCCGGAACATTCAGGAGTTGGGAAAGGAAGAAGAGGAGAACGAAGAGAAGCTTCGGAATGCGATCGAGTTGTTGGAGAGGGAGAAGAAGTTGATGGAGGAAGCTCCTGATTTGCAACTGGAGGACAATACCAAGCGGTTGAGACAGGCTTGTTTTAAGGCCAACTACAAGAAGAGAAAGATCATGGGGATGAATCTGATTGATTATCCACCCCATTCTTCTAATTTCAGCACCATTGATACTCGTGGAGGCAAGTCCATCGCTATTCTTGACAATCGAGGAGACGAGGAAGACGAAGAGATTAAGTCACTTTTATCTGACTCCTTCTCATCTTCCTCTAGCATGATGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

360

Amino Acids

41.8

Weight (kDa)

5.52

Isoelectric Point (pI)

73.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 193 - 240 1.6e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 669
AccIII TCCGGA 1 cut(s) 732
AciI CCGC 4 cut(s) 161, 247, 508, 865
AclWI GGATC 1 cut(s) 78
AcsI RAATTY 1 cut(s) 155
AfaI GTAC 4 cut(s) 16, 138, 550, 602
AfiI CCNNNNNNNGG 3 cut(s) 55, 679, 732
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 2 cut(s) 239, 661
AjnI CCWGG 1 cut(s) 256
AjuI GAANNNNNNNTTGG 2 cut(s) 88, 120
AluBI AGCT 3 cut(s) 558, 781, 833
AluI AGCT 3 cut(s) 558, 781, 833
Alw26I GTCTC 3 cut(s) 287, 865, 1005
AlwI GGATC 1 cut(s) 78
Aor13HI TCCGGA 1 cut(s) 732
AoxI GGCC 2 cut(s) 695, 888
ApeKI GCWGC 5 cut(s) 385, 388, 391, 415, 626
ApoI RAATTY 1 cut(s) 155
AseI ATTAAT 1 cut(s) 189
Asp700I GAANNNNTTC 1 cut(s) 559
AspLEI GCGC 1 cut(s) 342
AsuC2I CCSGG 1 cut(s) 674
AsuHPI GGTGA 3 cut(s) 11, 491, 703
BanI GGYRCC 1 cut(s) 669
BauI CACGAG 1 cut(s) 972
BbsI GAAGAC 2 cut(s) 212, 1026
BbvI GCAGC 5 cut(s) 397, 400, 403, 427, 638
BccI CCATC 4 cut(s) 30, 160, 817, 995
BcgI CGANNNNNNTGC 2 cut(s) 970, 1004
BciT130I CCWGG 1 cut(s) 258
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 1 cut(s) 674
BcoDI GTCTC 3 cut(s) 287, 865, 1005
BfaI CTAG 4 cut(s) 438, 552, 582, 1068
BfoI RGCGCY 1 cut(s) 343
BisI GCNGC 5 cut(s) 386, 389, 392, 416, 627
BlsI GCNGC 5 cut(s) 387, 390, 393, 417, 628
BmcAI AGTACT 2 cut(s) 16, 550
Bme1390I CCNGG 2 cut(s) 258, 674
BmiI GGNNCC 1 cut(s) 671
BmrFI CCNGG 2 cut(s) 258, 674
BmsI GCATC 1 cut(s) 184
BpiI GAAGAC 2 cut(s) 212, 1026
BplI GAGNNNNNCTC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 869
BpuMI CCSGG 1 cut(s) 674
BsaWI WCCGGW 2 cut(s) 133, 732
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 679, 732
Bse1I ACTGG 1 cut(s) 852
Bse3DI GCAATG 1 cut(s) 448
BseAI TCCGGA 1 cut(s) 732
BseBI CCWGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 924
BseLI CCNNNNNNNGG 3 cut(s) 55, 679, 732
BseMI GCAATG 1 cut(s) 448
BseMII CTCAG 2 cut(s) 288, 635
BseNI ACTGG 1 cut(s) 852
BseRI GAGGAG 3 cut(s) 25, 779, 1023
BseXI GCAGC 5 cut(s) 397, 400, 403, 427, 638
Bsh1285I CGRYCG 1 cut(s) 795
BshFI GGCC 2 cut(s) 697, 890
BshNI GGYRCC 1 cut(s) 669
BsiEI CGRYCG 1 cut(s) 795
BsiSI CCGG 3 cut(s) 134, 673, 733
BslI CCNNNNNNNGG 3 cut(s) 55, 679, 732
BsmAI GTCTC 3 cut(s) 287, 865, 1005
BsmBI CGTCTC 1 cut(s) 1005
BsmI GAATGC 1 cut(s) 793
BsnI GGCC 2 cut(s) 697, 890
Bsp13I TCCGGA 1 cut(s) 732
Bsp143I GATC 5 cut(s) 70, 448, 523, 792, 909
BspACI CCGC 4 cut(s) 161, 247, 508, 865
BspANI GGCC 2 cut(s) 697, 890
BspCNI CTCAG 2 cut(s) 289, 634
BspEI TCCGGA 1 cut(s) 732
BspHI TCATGA 2 cut(s) 73, 526
BspLI GGNNCC 1 cut(s) 671
BspPI GGATC 1 cut(s) 78
BspQI GCTCTTC 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 669
BsrDI GCAATG 1 cut(s) 448
BsrI ACTGG 1 cut(s) 852
BssMI GATC 5 cut(s) 70, 448, 523, 792, 909
BssSI CACGAG 1 cut(s) 972
Bst2BI CACGAG 1 cut(s) 972
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 314
Bst6I CTCTTC 8 cut(s) 204, 477, 491, 497, 756, 768, 896, 1020
BstDEI CTNAG 3 cut(s) 66, 297, 621
BstF5I GGATG 1 cut(s) 924
BstH2I RGCGCY 1 cut(s) 343
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 5 cut(s) 73, 451, 526, 795, 912
BstMAI GTCTC 3 cut(s) 287, 865, 1005
BstMBI GATC 5 cut(s) 70, 448, 523, 792, 909
BstMCI CGRYCG 1 cut(s) 795
BstMWI GCNNNNNNNGC 3 cut(s) 169, 385, 391
BstNI CCWGG 1 cut(s) 258
BstNSI RCATGY 2 cut(s) 476, 613
BstSCI CCNGG 2 cut(s) 256, 672
BstV1I GCAGC 5 cut(s) 397, 400, 403, 427, 638
BstV2I GAAGAC 2 cut(s) 212, 1026
BsuRI GGCC 2 cut(s) 697, 890
BtgZI GCGATG 1 cut(s) 973
BtsCI GGATG 1 cut(s) 924
BtsIMutI CAGTG 2 cut(s) 127, 319
CciI TCATGA 2 cut(s) 73, 526
CfoI GCGC 1 cut(s) 342
Csp6I GTAC 4 cut(s) 15, 137, 549, 601
CviAII CATG 8 cut(s) 74, 208, 473, 514, 527, 610, 913, 1072
CviJI RGCY 8 cut(s) 558, 648, 697, 717, 781, 833, 878, 890
CviKI_1 RGCY 8 cut(s) 558, 648, 697, 717, 781, 833, 878, 890
CviQI GTAC 4 cut(s) 15, 137, 549, 601
DdeI CTNAG 3 cut(s) 66, 297, 621
DpnI GATC 5 cut(s) 72, 450, 525, 794, 911
DpnII GATC 5 cut(s) 70, 448, 523, 792, 909
Eam1104I CTCTTC 8 cut(s) 204, 477, 491, 497, 756, 768, 896, 1020
EarI CTCTTC 8 cut(s) 204, 477, 491, 497, 756, 768, 896, 1020
EciI GGCGGA 2 cut(s) 150, 236
EcoRII CCWGG 1 cut(s) 256
Esp3I CGTCTC 1 cut(s) 1005
FaeI CATG 8 cut(s) 77, 211, 476, 517, 530, 613, 916, 1075
FalI AAGNNNNNCTT 2 cut(s) 765, 797
FatI CATG 8 cut(s) 73, 207, 472, 513, 526, 609, 912, 1071
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 5 cut(s) 386, 389, 392, 416, 627
FokI GGATG 1 cut(s) 931
Fsp4HI GCNGC 5 cut(s) 386, 389, 392, 416, 627
FspBI CTAG 4 cut(s) 438, 552, 582, 1068
GlaI GCGC 1 cut(s) 341
GluI GCNGC 5 cut(s) 386, 389, 392, 416, 627
GsuI CTGGAG 1 cut(s) 869
HaeII RGCGCY 1 cut(s) 343
HaeIII GGCC 2 cut(s) 697, 890
HapII CCGG 3 cut(s) 134, 673, 733
HhaI GCGC 1 cut(s) 342
Hin1II CATG 8 cut(s) 77, 211, 476, 517, 530, 613, 916, 1075
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 3 cut(s) 28, 922, 1049
HpaI GTTAAC 1 cut(s) 310
HpaII CCGG 3 cut(s) 134, 673, 733
HphI GGTGA 3 cut(s) 11, 491, 703
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 5 cut(s) 27, 705, 786, 927, 1048
Hpy188III TCNNGA 7 cut(s) 74, 527, 570, 733, 743, 836, 998
Hpy8I GTNNAC 1 cut(s) 310
Hpy99I CGWCG 2 cut(s) 571, 693
HpyAV CCTTC 3 cut(s) 234, 625, 1063
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4V TGCA 3 cut(s) 172, 513, 844
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 385, 391
HpyF3I CTNAG 3 cut(s) 66, 297, 621
Hsp92II CATG 8 cut(s) 77, 211, 476, 517, 530, 613, 916, 1075
HspAI GCGC 1 cut(s) 340
Kpn2I TCCGGA 1 cut(s) 732
Ksp22I TGATCA 1 cut(s) 523
KspAI GTTAAC 1 cut(s) 310
Kzo9I GATC 5 cut(s) 70, 448, 523, 792, 909
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 2 cut(s) 136, 838
Lsp1109I GCAGC 5 cut(s) 397, 400, 403, 427, 638
LweI GCATC 1 cut(s) 184
MaeI CTAG 4 cut(s) 438, 552, 582, 1068
MaeIII GTNAC 2 cut(s) 44, 1035
MalI GATC 5 cut(s) 72, 450, 525, 794, 911
MboI GATC 5 cut(s) 70, 448, 523, 792, 909
MluCI AATT 6 cut(s) 52, 101, 155, 424, 519, 952
MlyI GAGTC 2 cut(s) 37, 1043
MmeI TCCRAC 1 cut(s) 783
MroI TCCGGA 1 cut(s) 732
MroXI GAANNNNTTC 1 cut(s) 559
MseI TTAA 4 cut(s) 189, 309, 885, 1032
MslI CAYNNNNRTG 2 cut(s) 477, 917
MspI CCGG 3 cut(s) 134, 673, 733
MspR9I CCNGG 2 cut(s) 258, 674
Mva1269I GAATGC 1 cut(s) 793
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 3 cut(s) 169, 385, 391
NciI CCSGG 1 cut(s) 674
NdeII GATC 5 cut(s) 70, 448, 523, 792, 909
NlaIII CATG 8 cut(s) 77, 211, 476, 517, 530, 613, 916, 1075
NlaIV GGNNCC 1 cut(s) 671
NmuCI GTSAC 1 cut(s) 1035
NspI RCATGY 2 cut(s) 476, 613
PagI TCATGA 2 cut(s) 73, 526
PciI ACATGT 1 cut(s) 472
PciSI GCTCTTC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 1020
PctI GAATGC 1 cut(s) 793
PdmI GAANNNNTTC 1 cut(s) 559
PfeI GAWTC 1 cut(s) 922
PfoI TCCNGGA 1 cut(s) 256
PkrI GCNGC 5 cut(s) 387, 390, 393, 417, 628
Ple19I CGATCG 1 cut(s) 795
PleI GAGTC 2 cut(s) 36, 1043
PpsI GAGTC 2 cut(s) 36, 1043
PscI ACATGT 1 cut(s) 472
PshBI ATTAAT 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 671
PvuI CGATCG 1 cut(s) 795
RsaI GTAC 4 cut(s) 16, 138, 550, 602
RsaNI GTAC 4 cut(s) 15, 137, 549, 601
RseI CAYNNNNRTG 2 cut(s) 477, 917
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 4 cut(s) 189, 309, 885, 1032
SatI GCNGC 5 cut(s) 386, 389, 392, 416, 627
Sau3AI GATC 5 cut(s) 70, 448, 523, 792, 909
ScaI AGTACT 2 cut(s) 16, 550
SchI GAGTC 2 cut(s) 37, 1043
ScrFI CCNGG 2 cut(s) 258, 674
SetI ASST 5 cut(s) 424, 560, 671, 783, 835
SfaNI GCATC 1 cut(s) 184
SmiMI CAYNNNNRTG 2 cut(s) 477, 917
Sse9I AATT 6 cut(s) 52, 101, 155, 424, 519, 952
SsiI CCGC 4 cut(s) 161, 247, 508, 865
SspMI CTAG 4 cut(s) 438, 552, 582, 1068
StyD4I CCNGG 2 cut(s) 256, 672
TaaI ACNGT 1 cut(s) 314
TaqI TCGA 4 cut(s) 231, 569, 795, 1006
TasI AATT 6 cut(s) 52, 101, 155, 424, 519, 952
TatI WGTACW 2 cut(s) 14, 548
TfiI GAWTC 1 cut(s) 922
Tru1I TTAA 4 cut(s) 189, 309, 885, 1032
Tru9I TTAA 4 cut(s) 189, 309, 885, 1032
TscAI CASTG 2 cut(s) 127, 319
TseFI GTSAC 1 cut(s) 1035
TseI GCWGC 5 cut(s) 385, 388, 391, 415, 626
Tsp45I GTSAC 1 cut(s) 1035
TspDTI ATGAA 7 cut(s) 90, 168, 196, 459, 543, 552, 935
TspRI CASTG 2 cut(s) 127, 319
VspI ATTAAT 1 cut(s) 189
XapI RAATTY 1 cut(s) 155
XceI RCATGY 2 cut(s) 476, 613
XmnI GAANNNNTTC 1 cut(s) 559
XspI CTAG 4 cut(s) 438, 552, 582, 1068
ZrmI AGTACT 2 cut(s) 16, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.