Rw7G003610

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012997
Physical Location & Seq
Forward (+)
2959123 .. 2962033
2911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G003610.1

Sequence Viewer

Length: 1062 bp
ATGGCTAACATTGCTAAAGATGCTCTAAAAAGCTTCTTTTTTTTTTTGGTGAAATGTCATTTCCAAAAAGGAGGTGTTATTGCCATGCATGCTTGTATAAGTGGGTATGCTCCGGTACTTGATTGGGAAAATGAATTTCCGCCGATGGTGCAAGACTACACTTTTATTGATGCTCTTCCTTTCATGGAGAGTTGTCTTCCTTCGCTCGATATTCAACCTATCCGAAACCTATTTCCAGGAGATGATTTTTCCTTTGAAGATAATGGAAATGAGACTGAGAATGGAGTTAACAGTGAGTTGTTCGCTTCTGGGTTTGAGCGCCCTAATCATCATCATCAACAAGAACAAGAACAACAACAGCAGCAGCAGCAGCAGCAACAACATCAACAACAAGAAAAGCAGCAACCTAATTTACTGCTTCCTAGCAATGAAGATCAAAGTGGTGCTGATAGAGAACATGTGGGTGAACAGAGGAAGAGGAAGAGGCGGTGCATGAGAATTGATCATGAAGTGAAAAGTGGTAGTAGTACTAGAAGCTATTCATCGTCGAGATTGTTGTCTAGGGAAACAATCTCCTCGTACTTTTACATGCCTATAACTCAGGCAGCTAAGGAACTCAACATCGGCTTGACCCTTTTGAAGAAAAGGTGCCGGGAGTTGGGTATTCGTCGTTGGCCTCATCGGAAACTCACCAGCCTCCAAACCCTAATCCGGAACATTCAGGAGTTGGGAAAGGAAGAAGAGGAGAACGAAGAGAAGCTTCGGAATGCGATCGAGTTGTTGGAGAGGGAGAAGAAGTTGATGGAGGAAGCTCCTGATATGCAACTGGAGGATAATACCAAGCGGTTGAGACAGGCTTGTTTTAAGGCCAACTACAAGAAGAGAAAGATCATGGGGATGAATCTGATTGATTATCCACCGCATTCTTCTAATTTCAGCACCATTGATACTCGTGGAGGCAAGTCCGTCGCTATTCTTGACAATCGAGGAGACGAGGAGGACGAAGAGATTAAGTCACTTTTATCTGACTCCTTCTCATCTTCCTCTAGCATGATGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

353

Amino Acids

40.9

Weight (kDa)

5.88

Isoelectric Point (pI)

73.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 186 - 233 1.5e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 648
AccIII TCCGGA 1 cut(s) 711
AciI CCGC 4 cut(s) 140, 487, 844, 920
AcsI RAATTY 1 cut(s) 134
AfaI GTAC 3 cut(s) 117, 529, 581
AfiI CCNNNNNNNGG 2 cut(s) 658, 711
AflIII ACRYGT 1 cut(s) 457
AgsI TTSAA 3 cut(s) 215, 257, 640
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 5 cut(s) 33, 537, 608, 760, 812
AluI AGCT 5 cut(s) 33, 537, 608, 760, 812
Alw26I GTCTC 3 cut(s) 266, 844, 984
Aor13HI TCCGGA 1 cut(s) 711
AoxI GGCC 2 cut(s) 674, 867
ApeKI GCWGC 7 cut(s) 361, 364, 367, 370, 373, 400, 605
ApoI RAATTY 1 cut(s) 134
Asp700I GAANNNNTTC 1 cut(s) 538
AspLEI GCGC 1 cut(s) 321
AsuC2I CCSGG 1 cut(s) 653
AsuHPI GGTGA 3 cut(s) 61, 476, 682
BanI GGYRCC 1 cut(s) 648
BauI CACGAG 1 cut(s) 951
BbsI GAAGAC 1 cut(s) 188
BbvI GCAGC 7 cut(s) 373, 376, 379, 382, 385, 412, 617
BccI CCATC 2 cut(s) 139, 796
BcgI CGANNNNNNTGC 2 cut(s) 949, 983
BciT130I CCWGG 1 cut(s) 237
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 1 cut(s) 653
BcoDI GTCTC 3 cut(s) 266, 844, 984
BfaI CTAG 4 cut(s) 423, 531, 561, 1047
BfoI RGCGCY 1 cut(s) 322
BisI GCNGC 7 cut(s) 362, 365, 368, 371, 374, 401, 606
BlsI GCNGC 7 cut(s) 363, 366, 369, 372, 375, 402, 607
BmcAI AGTACT 1 cut(s) 529
Bme1390I CCNGG 2 cut(s) 237, 653
BmiI GGNNCC 1 cut(s) 650
BmrFI CCNGG 2 cut(s) 237, 653
BmsI GCATC 2 cut(s) 10, 160
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 848
Bpu10I CCTNAGC 1 cut(s) 609
BpuMI CCSGG 1 cut(s) 653
BsaWI WCCGGW 2 cut(s) 112, 711
BsaXI ACNNNNNCTCC 2 cut(s) 782, 812
Bsc4I CCNNNNNNNGG 2 cut(s) 658, 711
Bse1I ACTGG 1 cut(s) 831
Bse3DI GCAATG 2 cut(s) 9, 433
BseAI TCCGGA 1 cut(s) 711
BseBI CCWGG 1 cut(s) 237
BseGI GGATG 1 cut(s) 903
BseLI CCNNNNNNNGG 2 cut(s) 658, 711
BseMI GCAATG 2 cut(s) 9, 433
BseMII CTCAG 2 cut(s) 267, 614
BseNI ACTGG 1 cut(s) 831
BseRI GAGGAG 4 cut(s) 565, 758, 1002, 1010
BseXI GCAGC 7 cut(s) 373, 376, 379, 382, 385, 412, 617
Bsh1285I CGRYCG 1 cut(s) 774
BshFI GGCC 2 cut(s) 676, 869
BshNI GGYRCC 1 cut(s) 648
BsiEI CGRYCG 1 cut(s) 774
BsiSI CCGG 3 cut(s) 113, 652, 712
BslI CCNNNNNNNGG 2 cut(s) 658, 711
BsmAI GTCTC 3 cut(s) 266, 844, 984
BsmBI CGTCTC 1 cut(s) 984
BsmI GAATGC 2 cut(s) 772, 922
BsnI GGCC 2 cut(s) 676, 869
Bsp13I TCCGGA 1 cut(s) 711
Bsp143I GATC 4 cut(s) 433, 502, 771, 888
BspACI CCGC 4 cut(s) 140, 487, 844, 920
BspANI GGCC 2 cut(s) 676, 869
BspCNI CTCAG 2 cut(s) 268, 613
BspEI TCCGGA 1 cut(s) 711
BspHI TCATGA 1 cut(s) 505
BspLI GGNNCC 1 cut(s) 650
BspQI GCTCTTC 1 cut(s) 180
BspT107I GGYRCC 1 cut(s) 648
BsrDI GCAATG 2 cut(s) 9, 433
BsrI ACTGG 1 cut(s) 831
BssMI GATC 4 cut(s) 433, 502, 771, 888
BssSI CACGAG 1 cut(s) 951
Bst2BI CACGAG 1 cut(s) 951
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 293
Bst6I CTCTTC 7 cut(s) 180, 470, 476, 735, 747, 875, 999
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 3 cut(s) 276, 600, 609
BstF5I GGATG 1 cut(s) 903
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 1 cut(s) 321
BstKTI GATC 4 cut(s) 436, 505, 774, 891
BstMAI GTCTC 3 cut(s) 266, 844, 984
BstMBI GATC 4 cut(s) 433, 502, 771, 888
BstMCI CGRYCG 1 cut(s) 774
BstMWI GCNNNNNNNGC 7 cut(s) 11, 20, 89, 148, 367, 370, 373
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 3 cut(s) 92, 461, 592
BstSCI CCNGG 2 cut(s) 235, 651
BstV1I GCAGC 7 cut(s) 373, 376, 379, 382, 385, 412, 617
BstV2I GAAGAC 1 cut(s) 188
BsuRI GGCC 2 cut(s) 676, 869
BtsCI GGATG 1 cut(s) 903
BtsIMutI CAGTG 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 90
CciI TCATGA 1 cut(s) 505
CfoI GCGC 1 cut(s) 321
Csp6I GTAC 3 cut(s) 116, 528, 580
CviAII CATG 9 cut(s) 85, 89, 184, 458, 493, 506, 589, 892, 1051
CviQI GTAC 3 cut(s) 116, 528, 580
DdeI CTNAG 3 cut(s) 276, 600, 609
DpnI GATC 4 cut(s) 435, 504, 773, 890
DpnII GATC 4 cut(s) 433, 502, 771, 888
Eam1104I CTCTTC 7 cut(s) 180, 470, 476, 735, 747, 875, 999
EarI CTCTTC 7 cut(s) 180, 470, 476, 735, 747, 875, 999
EciI GGCGGA 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 235
EcoT22I ATGCAT 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 984
FaeI CATG 9 cut(s) 88, 92, 187, 461, 496, 509, 592, 895, 1054
FalI AAGNNNNNCTT 2 cut(s) 744, 776
FatI CATG 9 cut(s) 84, 88, 183, 457, 492, 505, 588, 891, 1050
FbaI TGATCA 1 cut(s) 502
Fnu4HI GCNGC 7 cut(s) 362, 365, 368, 371, 374, 401, 606
FokI GGATG 1 cut(s) 910
Fsp4HI GCNGC 7 cut(s) 362, 365, 368, 371, 374, 401, 606
FspBI CTAG 4 cut(s) 423, 531, 561, 1047
GlaI GCGC 1 cut(s) 320
GluI GCNGC 7 cut(s) 362, 365, 368, 371, 374, 401, 606
GsuI CTGGAG 1 cut(s) 848
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 2 cut(s) 676, 869
HapII CCGG 3 cut(s) 113, 652, 712
HhaI GCGC 1 cut(s) 321
Hin1II CATG 9 cut(s) 88, 92, 187, 461, 496, 509, 592, 895, 1054
Hin6I GCGC 1 cut(s) 319
HinP1I GCGC 1 cut(s) 319
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HindIII AAGCTT 2 cut(s) 31, 758
HinfI GANTC 2 cut(s) 901, 1028
HpaI GTTAAC 1 cut(s) 289
HpaII CCGG 3 cut(s) 113, 652, 712
HphI GGTGA 3 cut(s) 61, 476, 682
Hpy166II GTNNAC 2 cut(s) 289, 467
Hpy188I TCNGA 5 cut(s) 224, 684, 765, 906, 1027
Hpy188III TCNNGA 6 cut(s) 506, 549, 712, 722, 815, 977
Hpy8I GTNNAC 2 cut(s) 289, 467
Hpy99I CGWCG 3 cut(s) 550, 672, 971
HpyAV CCTTC 2 cut(s) 210, 1042
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4V TGCA 4 cut(s) 88, 151, 492, 823
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 20, 89, 148, 367, 370, 373
HpyF3I CTNAG 3 cut(s) 276, 600, 609
Hsp92II CATG 9 cut(s) 88, 92, 187, 461, 496, 509, 592, 895, 1054
HspAI GCGC 1 cut(s) 319
Kpn2I TCCGGA 1 cut(s) 711
Ksp22I TGATCA 1 cut(s) 502
KspAI GTTAAC 1 cut(s) 289
Kzo9I GATC 4 cut(s) 433, 502, 771, 888
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 115, 817
Lsp1109I GCAGC 7 cut(s) 373, 376, 379, 382, 385, 412, 617
LweI GCATC 2 cut(s) 10, 160
MaeI CTAG 4 cut(s) 423, 531, 561, 1047
MaeIII GTNAC 1 cut(s) 1014
MalI GATC 4 cut(s) 435, 504, 773, 890
MboI GATC 4 cut(s) 433, 502, 771, 888
MluCI AATT 4 cut(s) 134, 409, 498, 931
MlyI GAGTC 1 cut(s) 1022
MmeI TCCRAC 1 cut(s) 762
Mph1103I ATGCAT 1 cut(s) 90
MroI TCCGGA 1 cut(s) 711
MroXI GAANNNNTTC 1 cut(s) 538
MseI TTAA 3 cut(s) 288, 864, 1011
MslI CAYNNNNRTG 2 cut(s) 462, 896
MspI CCGG 3 cut(s) 113, 652, 712
MspR9I CCNGG 2 cut(s) 237, 653
Mva1269I GAATGC 2 cut(s) 772, 922
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 7 cut(s) 11, 20, 89, 148, 367, 370, 373
NciI CCSGG 1 cut(s) 653
NdeII GATC 4 cut(s) 433, 502, 771, 888
NlaIII CATG 9 cut(s) 88, 92, 187, 461, 496, 509, 592, 895, 1054
NlaIV GGNNCC 1 cut(s) 650
NmuCI GTSAC 1 cut(s) 1014
NsiI ATGCAT 1 cut(s) 90
NspI RCATGY 3 cut(s) 92, 461, 592
PaeI GCATGC 1 cut(s) 92
PagI TCATGA 1 cut(s) 505
PciI ACATGT 1 cut(s) 457
PciSI GCTCTTC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 999
PctI GAATGC 2 cut(s) 772, 922
PdmI GAANNNNTTC 1 cut(s) 538
PfeI GAWTC 1 cut(s) 901
PfoI TCCNGGA 1 cut(s) 235
PkrI GCNGC 7 cut(s) 363, 366, 369, 372, 375, 402, 607
Ple19I CGATCG 1 cut(s) 774
PleI GAGTC 1 cut(s) 1022
PpsI GAGTC 1 cut(s) 1022
PscI ACATGT 1 cut(s) 457
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 650
PvuI CGATCG 1 cut(s) 774
RsaI GTAC 3 cut(s) 117, 529, 581
RsaNI GTAC 3 cut(s) 116, 528, 580
RseI CAYNNNNRTG 2 cut(s) 462, 896
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 3 cut(s) 288, 864, 1011
SatI GCNGC 7 cut(s) 362, 365, 368, 371, 374, 401, 606
Sau3AI GATC 4 cut(s) 433, 502, 771, 888
ScaI AGTACT 1 cut(s) 529
SchI GAGTC 1 cut(s) 1022
ScrFI CCNGG 2 cut(s) 237, 653
SfaNI GCATC 2 cut(s) 10, 160
SmiMI CAYNNNNRTG 2 cut(s) 462, 896
SphI GCATGC 1 cut(s) 92
Sse9I AATT 4 cut(s) 134, 409, 498, 931
SsiI CCGC 4 cut(s) 140, 487, 844, 920
SspMI CTAG 4 cut(s) 423, 531, 561, 1047
StyD4I CCNGG 2 cut(s) 235, 651
TaaI ACNGT 1 cut(s) 293
TaqI TCGA 4 cut(s) 207, 548, 774, 985
TasI AATT 4 cut(s) 134, 409, 498, 931
TatI WGTACW 1 cut(s) 527
TfiI GAWTC 1 cut(s) 901
Tru1I TTAA 3 cut(s) 288, 864, 1011
Tru9I TTAA 3 cut(s) 288, 864, 1011
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 1 cut(s) 1014
TseI GCWGC 7 cut(s) 361, 364, 367, 370, 373, 400, 605
Tsp45I GTSAC 1 cut(s) 1014
TspDTI ATGAA 6 cut(s) 147, 172, 444, 522, 531, 914
TspGWI ACGGA 1 cut(s) 955
TspRI CASTG 1 cut(s) 298
XapI RAATTY 1 cut(s) 134
XceI RCATGY 3 cut(s) 92, 461, 592
XmnI GAANNNNTTC 1 cut(s) 538
XspI CTAG 4 cut(s) 423, 531, 561, 1047
ZrmI AGTACT 1 cut(s) 529
Zsp2I ATGCAT 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.