RchiOBHm_Chr7g0181471

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012997
Physical Location & Seq
Forward (+)
3114046 .. 3115174
1129 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16185

Sequence Viewer

Length: 987 bp
ATGGTGCAAGACTACACTTTTATTGATGCTCTTCCTTTCATGGAGAGTTGTCTTCCTTCGCTCGATATTCAACCTATCCGACACCTATTTCCAGGAGATGATTTTTCCTTTGGAGATAATGGAAATGAGACTGAGAATGGAGTTAACAGTGAGTTGTTCGCTTCTGGGTTTGAGCGCCCTAATCATCATCATCAACAAGAACAAGAACAACAACAGCAGCAGCAGCAGCAACAGCAACAACAAGAAAAGCAGCAACCTAATTTACTGCTTCCTAGCAATGAAGATCAAAGTGGTGCTGATAGAGAACATGTGGCTGAAGAGAGGAAGAGGAAGAGGCGGTGCATGAGAATTGATCATGAAGTGAAAAGTGGTAGTAGTACTAGAAGCTATTCATCGTCGAGATTGTTGTCTAGGGAAACAATCTCCTCGTACTTTTACATGCCTATAACTCAGGCAGCTAAGGAACTCAACATCGGCTTGACCCTTTTGAAGAAAAGGTGCAGGGAGTTGGGTATTCGTCGTTGGCCTCATCGGAAACTCACCAGCCTCCAAACCCTAATCCGGAACATTCAGGTACCGAAGTACGACATATTTCAAAAAGAAAATTTCACCATTGGAATATATAATTTTCTAAAGCAAGTGATGATTAATTTGGGAAAGGAAGAAGAGGAGAACGAAGAGAAGCTTCGGAATGCGATCGAGTTGTTGGAGAGGGAGAAGAAGTTGATGGAGGAAGCTCCTGATATGCAACTGGAGGATAATACCAAGCGGTTGAGACAGGCTTGTTTTAAGGCCAACTACAAGAAGAGAAAGATCATGGGGATGAATCTGATTGATTATCCACCGCATTCTTCTAATTTCAGCACCATTGATACTCGTGGAGGCAAGTCCGTCGCTATTCTTGACAATCGAGGAGAGGAGGAGGACGAAGAGATTAAGTCACTTTTATCTGACTCCTTCTCATCTTCCTCTAGCATGATGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

328

Amino Acids

38.26

Weight (kDa)

5.99

Isoelectric Point (pI)

70.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 136 - 183 1.3e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 574
AccB1I GGYRCC 1 cut(s) 574
AccIII TCCGGA 1 cut(s) 561
AciI CCGC 3 cut(s) 337, 769, 845
AcsI RAATTY 1 cut(s) 604
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 4 cut(s) 379, 431, 576, 584
AfiI CCNNNNNNNGG 1 cut(s) 561
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 3 cut(s) 71, 490, 596
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 4 cut(s) 387, 458, 685, 737
AluI AGCT 4 cut(s) 387, 458, 685, 737
Alw26I GTCTC 2 cut(s) 122, 769
Aor13HI TCCGGA 1 cut(s) 561
AoxI GGCC 2 cut(s) 524, 792
ApeKI GCWGC 6 cut(s) 217, 220, 223, 226, 250, 455
ApoI RAATTY 1 cut(s) 604
AseI ATTAAT 1 cut(s) 648
Asp700I GAANNNNTTC 1 cut(s) 388
Asp718I GGTACC 1 cut(s) 574
AspLEI GCGC 1 cut(s) 177
AsuHPI GGTGA 2 cut(s) 532, 601
BaeI ACNNNNGTAYC 2 cut(s) 566, 599
BanI GGYRCC 1 cut(s) 574
BauI CACGAG 1 cut(s) 876
BbsI GAAGAC 1 cut(s) 44
BbvI GCAGC 6 cut(s) 229, 232, 235, 238, 262, 467
BccI CCATC 1 cut(s) 721
BcgI CGANNNNNNTGC 2 cut(s) 874, 908
BciT130I CCWGG 1 cut(s) 93
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 2 cut(s) 122, 769
BfaI CTAG 4 cut(s) 273, 381, 411, 972
BfoI RGCGCY 1 cut(s) 178
BisI GCNGC 6 cut(s) 218, 221, 224, 227, 251, 456
BlsI GCNGC 6 cut(s) 219, 222, 225, 228, 252, 457
BmcAI AGTACT 1 cut(s) 379
Bme1390I CCNGG 1 cut(s) 93
BmiI GGNNCC 1 cut(s) 576
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 16
BpiI GAAGAC 1 cut(s) 44
BpmI CTGGAG 1 cut(s) 773
Bpu10I CCTNAGC 1 cut(s) 459
BsaWI WCCGGW 1 cut(s) 561
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bsc4I CCNNNNNNNGG 1 cut(s) 561
Bse1I ACTGG 1 cut(s) 756
Bse3DI GCAATG 1 cut(s) 283
BseAI TCCGGA 1 cut(s) 561
BseBI CCWGG 1 cut(s) 93
BseGI GGATG 1 cut(s) 828
BseLI CCNNNNNNNGG 1 cut(s) 561
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 2 cut(s) 123, 464
BseNI ACTGG 1 cut(s) 756
BseRI GAGGAG 5 cut(s) 415, 683, 927, 932, 935
BseXI GCAGC 6 cut(s) 229, 232, 235, 238, 262, 467
BsgI GTGCAG 1 cut(s) 520
Bsh1285I CGRYCG 1 cut(s) 699
BshFI GGCC 2 cut(s) 526, 794
BshNI GGYRCC 1 cut(s) 574
BsiEI CGRYCG 1 cut(s) 699
BsiSI CCGG 1 cut(s) 562
BslI CCNNNNNNNGG 1 cut(s) 561
BsmAI GTCTC 2 cut(s) 122, 769
BsmI GAATGC 2 cut(s) 697, 847
BsnI GGCC 2 cut(s) 526, 794
Bsp13I TCCGGA 1 cut(s) 561
Bsp143I GATC 4 cut(s) 283, 352, 696, 813
BspACI CCGC 3 cut(s) 337, 769, 845
BspANI GGCC 2 cut(s) 526, 794
BspCNI CTCAG 2 cut(s) 124, 463
BspEI TCCGGA 1 cut(s) 561
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 576
BspQI GCTCTTC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 574
BsrDI GCAATG 1 cut(s) 283
BsrI ACTGG 1 cut(s) 756
BssMI GATC 4 cut(s) 283, 352, 696, 813
BssSI CACGAG 1 cut(s) 876
Bst2BI CACGAG 1 cut(s) 876
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 1 cut(s) 149
Bst6I CTCTTC 8 cut(s) 36, 312, 320, 326, 660, 672, 800, 924
BstDEI CTNAG 3 cut(s) 132, 450, 459
BstF5I GGATG 1 cut(s) 828
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 4 cut(s) 286, 355, 699, 816
BstMAI GTCTC 2 cut(s) 122, 769
BstMBI GATC 4 cut(s) 283, 352, 696, 813
BstMCI CGRYCG 1 cut(s) 699
BstMWI GCNNNNNNNGC 3 cut(s) 223, 226, 232
BstNI CCWGG 1 cut(s) 93
BstNSI RCATGY 2 cut(s) 311, 442
BstSCI CCNGG 1 cut(s) 91
BstV1I GCAGC 6 cut(s) 229, 232, 235, 238, 262, 467
BstV2I GAAGAC 1 cut(s) 44
BsuRI GGCC 2 cut(s) 526, 794
BtsCI GGATG 1 cut(s) 828
BtsIMutI CAGTG 1 cut(s) 154
CciI TCATGA 1 cut(s) 355
CfoI GCGC 1 cut(s) 177
Csp6I GTAC 4 cut(s) 378, 430, 575, 583
CviAII CATG 7 cut(s) 40, 308, 343, 356, 439, 817, 976
CviQI GTAC 4 cut(s) 378, 430, 575, 583
DdeI CTNAG 3 cut(s) 132, 450, 459
DpnI GATC 4 cut(s) 285, 354, 698, 815
DpnII GATC 4 cut(s) 283, 352, 696, 813
Eam1104I CTCTTC 8 cut(s) 36, 312, 320, 326, 660, 672, 800, 924
EarI CTCTTC 8 cut(s) 36, 312, 320, 326, 660, 672, 800, 924
Eco57I CTGAAG 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 91
FaeI CATG 7 cut(s) 43, 311, 346, 359, 442, 820, 979
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FatI CATG 7 cut(s) 39, 307, 342, 355, 438, 816, 975
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 6 cut(s) 218, 221, 224, 227, 251, 456
FokI GGATG 1 cut(s) 835
Fsp4HI GCNGC 6 cut(s) 218, 221, 224, 227, 251, 456
FspBI CTAG 4 cut(s) 273, 381, 411, 972
GlaI GCGC 1 cut(s) 176
GluI GCNGC 6 cut(s) 218, 221, 224, 227, 251, 456
GsuI CTGGAG 1 cut(s) 773
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 2 cut(s) 526, 794
HapII CCGG 1 cut(s) 562
HhaI GCGC 1 cut(s) 177
Hin1II CATG 7 cut(s) 43, 311, 346, 359, 442, 820, 979
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 2 cut(s) 826, 953
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 1 cut(s) 562
HphI GGTGA 2 cut(s) 532, 601
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 5 cut(s) 80, 534, 690, 831, 952
Hpy188III TCNNGA 5 cut(s) 356, 399, 562, 740, 902
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 3 cut(s) 400, 522, 896
HpyAV CCTTC 2 cut(s) 66, 967
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4V TGCA 4 cut(s) 7, 342, 501, 748
HpyF10VI GCNNNNNNNGC 3 cut(s) 223, 226, 232
HpyF3I CTNAG 3 cut(s) 132, 450, 459
Hsp92II CATG 7 cut(s) 43, 311, 346, 359, 442, 820, 979
HspAI GCGC 1 cut(s) 175
Kpn2I TCCGGA 1 cut(s) 561
KpnI GGTACC 1 cut(s) 578
Ksp22I TGATCA 1 cut(s) 352
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 4 cut(s) 283, 352, 696, 813
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 1 cut(s) 742
Lsp1109I GCAGC 6 cut(s) 229, 232, 235, 238, 262, 467
LweI GCATC 1 cut(s) 16
MaeI CTAG 4 cut(s) 273, 381, 411, 972
MaeIII GTNAC 1 cut(s) 939
MalI GATC 4 cut(s) 285, 354, 698, 815
MboI GATC 4 cut(s) 283, 352, 696, 813
MluCI AATT 6 cut(s) 259, 348, 604, 625, 649, 856
MlyI GAGTC 1 cut(s) 947
MmeI TCCRAC 2 cut(s) 103, 687
MroI TCCGGA 1 cut(s) 561
MroXI GAANNNNTTC 1 cut(s) 388
MseI TTAA 4 cut(s) 144, 648, 789, 936
MslI CAYNNNNRTG 1 cut(s) 821
MspI CCGG 1 cut(s) 562
MspR9I CCNGG 1 cut(s) 93
Mva1269I GAATGC 2 cut(s) 697, 847
MvaI CCWGG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 223, 226, 232
NdeII GATC 4 cut(s) 283, 352, 696, 813
NlaIII CATG 7 cut(s) 43, 311, 346, 359, 442, 820, 979
NlaIV GGNNCC 1 cut(s) 576
NmuCI GTSAC 1 cut(s) 939
NspI RCATGY 2 cut(s) 311, 442
PagI TCATGA 1 cut(s) 355
PciI ACATGT 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 36
PctI GAATGC 2 cut(s) 697, 847
PdmI GAANNNNTTC 1 cut(s) 388
PfeI GAWTC 1 cut(s) 826
PfoI TCCNGGA 1 cut(s) 91
PkrI GCNGC 6 cut(s) 219, 222, 225, 228, 252, 457
Ple19I CGATCG 1 cut(s) 699
PleI GAGTC 1 cut(s) 947
PpsI GAGTC 1 cut(s) 947
PscI ACATGT 1 cut(s) 307
PshBI ATTAAT 1 cut(s) 648
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 576
PvuI CGATCG 1 cut(s) 699
RsaI GTAC 4 cut(s) 379, 431, 576, 584
RsaNI GTAC 4 cut(s) 378, 430, 575, 583
RseI CAYNNNNRTG 1 cut(s) 821
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 4 cut(s) 144, 648, 789, 936
SatI GCNGC 6 cut(s) 218, 221, 224, 227, 251, 456
Sau3AI GATC 4 cut(s) 283, 352, 696, 813
ScaI AGTACT 1 cut(s) 379
SchI GAGTC 1 cut(s) 947
ScrFI CCNGG 1 cut(s) 93
SetI ASST 9 cut(s) 76, 87, 259, 389, 460, 500, 576, 687, 739
SfaNI GCATC 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 821
Sse9I AATT 6 cut(s) 259, 348, 604, 625, 649, 856
SsiI CCGC 3 cut(s) 337, 769, 845
SspMI CTAG 4 cut(s) 273, 381, 411, 972
StyD4I CCNGG 1 cut(s) 91
TaaI ACNGT 1 cut(s) 149
TaqI TCGA 4 cut(s) 63, 398, 699, 910
TasI AATT 6 cut(s) 259, 348, 604, 625, 649, 856
TatI WGTACW 1 cut(s) 377
TfiI GAWTC 1 cut(s) 826
Tru1I TTAA 4 cut(s) 144, 648, 789, 936
Tru9I TTAA 4 cut(s) 144, 648, 789, 936
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 939
TseI GCWGC 6 cut(s) 217, 220, 223, 226, 250, 455
Tsp45I GTSAC 1 cut(s) 939
TspDTI ATGAA 5 cut(s) 28, 294, 372, 381, 839
TspGWI ACGGA 1 cut(s) 880
TspRI CASTG 1 cut(s) 154
VspI ATTAAT 1 cut(s) 648
XapI RAATTY 1 cut(s) 604
XceI RCATGY 2 cut(s) 311, 442
XmnI GAANNNNTTC 1 cut(s) 388
XspI CTAG 4 cut(s) 273, 381, 411, 972
ZrmI AGTACT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.