FvH4_5g14910

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012997
Physical Location & Seq
Reverse (-)
8439412 .. 8440768
1357 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14910.t1

Sequence Viewer

Length: 1080 bp
ATGACAGTGAAGAGTCACCATCAGAGTCATTCCCTCATGAGTAACCCTAATTGGGTGGGGGTTTCCAATGAAAACCCATTCTCATTTGCAAATCAATTTTCTCCTGCTGATTTCAGTGGTTATGCTCCAGTACTGGACTGGGAAAATGAAGTTCCGCCGATGGTGCAAGACTATAGTTTTGTTGACGCTCTTCCTTTCATGGAGACCTGTCTGAATCTCCATGATCACGACCCTCTTTACTCGTCTCTTGACATACAACCTATCCGAAGCCTATTTCAAGGAGATGATGTTTTGTTTGGAGACAATGCAAATGAGACTGAGAATGTAGTTCACAATGAGGTGTTCGCTTCTGGGTTTGAGCACCCTAATCATGATCATGATCAACAAGAACAACAGCAACAAGAAAAGCAGCAGCCTATTTTACTGCTTACGAGCAATGAAGATCAAAATGGCGGAGATAGAGAACATGTCGGTGAAGAGAGGAAGCATAAGAAGCGATGCATGAGAGACGAAGTGAAAAGTGGTAGCAGTACTCGAAGCTATTCATCATCAAAATTGTTGTCTAGGGAAACAATCTCCCAGTACTTTTACATGCCTATAACTCAAGCAGCGAAAGAACTAAACGTCGGCTTGACTCTTTTGAAGAAAAGGTGCCGGGAGTTGGGTGTTCGCCGTTGGCCTCATCGGAAACTCACCAGCCTCCAGACCCTAATCCGGAACATTCAGGTGAACATTCAGGAGTTGGGAAAGGAAGAAGATGACAATAATGAAGAGAAGCTTCGGAATGCGATCGAGTTATTGGAGAGGGAGAAGAAGCTGATGGAGGAAGCTCCGGATATGCAACTGGAGGACCATACCAAGCGGTTGAGACAGGCTTGTTTTAAGGCCAACTACAAGAAGAGAAAGATCATGGGGATGAATCTGATTGATTATCCACCCCATTCTTCTAATTTCAGCATTGTTGATAATCGTGGAGGGAAGTCCATGGCTATTATTGACAATCGAGGAGACGAGGAAGACGAAGAGATTAAGTCGCTTTTGTCTGATTCCTTTTCATCTTCCGCTAGCATGATGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

360

Amino Acids

41.34

Weight (kDa)

5.36

Isoelectric Point (pI)

55.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 187 - 234 7.7e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 651
AccIII TCCGGA 2 cut(s) 714, 832
AciI CCGC 4 cut(s) 155, 453, 862, 1062
AfaI GTAC 3 cut(s) 132, 532, 584
AfiI CCNNNNNNNGG 3 cut(s) 52, 661, 714
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 2 cut(s) 278, 643
AluBI AGCT 4 cut(s) 540, 778, 817, 830
AluI AGCT 4 cut(s) 540, 778, 817, 830
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 7 cut(s) 197, 249, 294, 308, 501, 862, 1002
Aor13HI TCCGGA 2 cut(s) 714, 832
AoxI GGCC 2 cut(s) 677, 885
ApeKI GCWGC 3 cut(s) 409, 412, 608
ArsI GACNNNNNNTTYG 4 cut(s) 161, 193, 545, 577
Asp700I GAANNNNTTC 1 cut(s) 541
AspS9I GGNCC 1 cut(s) 850
AsuC2I CCSGG 1 cut(s) 656
AsuHPI GGTGA 4 cut(s) 8, 485, 685, 739
AsuNHI GCTAGC 1 cut(s) 1064
AvaII GGWCC 1 cut(s) 850
BanI GGYRCC 1 cut(s) 651
BbsI GAAGAC 1 cut(s) 1023
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 3 cut(s) 421, 424, 620
BccI CCATC 3 cut(s) 27, 154, 814
BceAI ACGGC 1 cut(s) 657
BclI TGATCA 3 cut(s) 223, 373, 379
BcnI CCSGG 1 cut(s) 656
BcoDI GTCTC 7 cut(s) 197, 249, 294, 308, 501, 862, 1002
BfaI CTAG 2 cut(s) 564, 1065
BfmI CTRYAG 1 cut(s) 172
BisI GCNGC 3 cut(s) 410, 413, 609
BlsI GCNGC 3 cut(s) 411, 414, 610
BmcAI AGTACT 3 cut(s) 132, 532, 584
Bme1390I CCNGG 1 cut(s) 656
Bme18I GGWCC 1 cut(s) 850
BmgT120I GGNCC 1 cut(s) 850
BmiI GGNNCC 1 cut(s) 653
BmrFI CCNGG 1 cut(s) 656
BmrI ACTGGG 2 cut(s) 148, 574
BmsI GCATC 1 cut(s) 488
BmtI GCTAGC 1 cut(s) 1068
BmuI ACTGGG 2 cut(s) 148, 574
BpiI GAAGAC 1 cut(s) 1023
BpmI CTGGAG 3 cut(s) 111, 686, 866
BpuEI CTTGAG 1 cut(s) 588
BpuMI CCSGG 1 cut(s) 656
BsaBI GATNNNNATC 1 cut(s) 378
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 984
BsaWI WCCGGW 2 cut(s) 714, 832
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 661, 714
Bse1I ACTGG 5 cut(s) 128, 138, 143, 580, 849
Bse3DI GCAATG 1 cut(s) 442
Bse8I GATNNNNATC 1 cut(s) 378
BseAI TCCGGA 2 cut(s) 714, 832
BseDI CCNNGG 1 cut(s) 984
BseGI GGATG 1 cut(s) 921
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 3 cut(s) 52, 661, 714
BseMI GCAATG 1 cut(s) 442
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 5 cut(s) 128, 138, 143, 580, 849
BseRI GAGGAG 1 cut(s) 1020
BseXI GCAGC 3 cut(s) 421, 424, 620
Bsh1285I CGRYCG 1 cut(s) 792
BshFI GGCC 2 cut(s) 679, 887
BshNI GGYRCC 1 cut(s) 651
BsiEI CGRYCG 1 cut(s) 792
BsiHKAI GWGCWC 1 cut(s) 363
BsiSI CCGG 3 cut(s) 655, 715, 833
BslI CCNNNNNNNGG 3 cut(s) 52, 661, 714
BsmAI GTCTC 7 cut(s) 197, 249, 294, 308, 501, 862, 1002
BsmBI CGTCTC 3 cut(s) 249, 501, 1002
BsmI GAATGC 1 cut(s) 790
BsnI GGCC 2 cut(s) 679, 887
Bso31I GGTCTC 1 cut(s) 197
Bsp1286I GDGCHC 1 cut(s) 363
Bsp13I TCCGGA 2 cut(s) 714, 832
Bsp143I GATC 6 cut(s) 223, 373, 379, 442, 789, 906
Bsp19I CCATGG 1 cut(s) 984
BspACI CCGC 4 cut(s) 155, 453, 862, 1062
BspANI GGCC 2 cut(s) 679, 887
BspCNI CTCAG 1 cut(s) 310
BspEI TCCGGA 2 cut(s) 714, 832
BspHI TCATGA 3 cut(s) 36, 370, 376
BspLI GGNNCC 1 cut(s) 653
BspOI GCTAGC 1 cut(s) 1068
BspQI GCTCTTC 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 651
BspTNI GGTCTC 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 442
BsrI ACTGG 5 cut(s) 128, 138, 143, 580, 849
BssECI CCNNGG 1 cut(s) 984
BssMI GATC 6 cut(s) 223, 373, 379, 442, 789, 906
BssT1I CCWWGG 1 cut(s) 984
Bst4CI ACNGT 1 cut(s) 7
Bst6I CTCTTC 6 cut(s) 5, 195, 471, 765, 893, 1017
BstC8I GCNNGC 1 cut(s) 1066
BstDEI CTNAG 1 cut(s) 318
BstDSI CCRYGG 1 cut(s) 984
BstF5I GGATG 1 cut(s) 921
BstKTI GATC 6 cut(s) 226, 376, 382, 445, 792, 909
BstMAI GTCTC 7 cut(s) 197, 249, 294, 308, 501, 862, 1002
BstMBI GATC 6 cut(s) 223, 373, 379, 442, 789, 906
BstMCI CGRYCG 1 cut(s) 792
BstMWI GCNNNNNNNGC 2 cut(s) 163, 493
BstNSI RCATGY 2 cut(s) 470, 595
BstSCI CCNGG 1 cut(s) 654
BstSFI CTRYAG 1 cut(s) 172
BstV1I GCAGC 3 cut(s) 421, 424, 620
BstV2I GAAGAC 1 cut(s) 1023
BsuRI GGCC 2 cut(s) 679, 887
BtgI CCRYGG 1 cut(s) 984
BtgZI GCGATG 1 cut(s) 511
BtsCI GGATG 1 cut(s) 921
BtsIMutI CAGTG 2 cut(s) 12, 121
Cac8I GCNNGC 1 cut(s) 1066
CciI TCATGA 3 cut(s) 36, 370, 376
Cfr13I GGNCC 1 cut(s) 850
CseI GACGC 1 cut(s) 194
Csp6I GTAC 3 cut(s) 131, 531, 583
CviQI GTAC 3 cut(s) 131, 531, 583
DdeI CTNAG 1 cut(s) 318
DpnI GATC 6 cut(s) 225, 375, 381, 444, 791, 908
DpnII GATC 6 cut(s) 223, 373, 379, 442, 789, 906
Eam1104I CTCTTC 6 cut(s) 5, 195, 471, 765, 893, 1017
EarI CTCTTC 6 cut(s) 5, 195, 471, 765, 893, 1017
EciI GGCGGA 2 cut(s) 144, 468
Eco130I CCWWGG 1 cut(s) 984
Eco31I GGTCTC 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 850
EcoT14I CCWWGG 1 cut(s) 984
EcoT22I ATGCAT 1 cut(s) 503
ErhI CCWWGG 1 cut(s) 984
Esp3I CGTCTC 3 cut(s) 249, 501, 1002
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FbaI TGATCA 3 cut(s) 223, 373, 379
Fnu4HI GCNGC 3 cut(s) 410, 413, 609
FokI GGATG 1 cut(s) 928
Fsp4HI GCNGC 3 cut(s) 410, 413, 609
FspBI CTAG 2 cut(s) 564, 1065
GluI GCNGC 3 cut(s) 410, 413, 609
GsuI CTGGAG 3 cut(s) 111, 686, 866
HaeIII GGCC 2 cut(s) 679, 887
HapII CCGG 3 cut(s) 655, 715, 833
HgaI GACGC 1 cut(s) 194
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HindIII AAGCTT 1 cut(s) 776
HinfI GANTC 6 cut(s) 13, 25, 214, 634, 919, 1046
HpaII CCGG 3 cut(s) 655, 715, 833
HphI GGTGA 4 cut(s) 8, 485, 685, 739
Hpy166II GTNNAC 3 cut(s) 184, 331, 730
Hpy188I TCNGA 7 cut(s) 24, 213, 266, 687, 783, 924, 1045
Hpy188III TCNNGA 9 cut(s) 37, 227, 248, 371, 377, 703, 715, 737, 833
Hpy8I GTNNAC 3 cut(s) 184, 331, 730
Hpy99I CGWCG 1 cut(s) 629
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4IV ACGT 1 cut(s) 624
HpyCH4V TGCA 5 cut(s) 89, 166, 308, 501, 841
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 493
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 624
Kpn2I TCCGGA 2 cut(s) 714, 832
Ksp22I TGATCA 3 cut(s) 223, 373, 379
Kzo9I GATC 6 cut(s) 223, 373, 379, 442, 789, 906
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 2 cut(s) 130, 835
Lsp1109I GCAGC 3 cut(s) 421, 424, 620
LweI GCATC 1 cut(s) 488
MaeI CTAG 2 cut(s) 564, 1065
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 2 cut(s) 14, 41
MalI GATC 6 cut(s) 225, 375, 381, 444, 791, 908
MboI GATC 6 cut(s) 223, 373, 379, 442, 789, 906
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 4 cut(s) 49, 95, 554, 949
MlyI GAGTC 3 cut(s) 22, 34, 628
Mph1103I ATGCAT 1 cut(s) 503
MroI TCCGGA 2 cut(s) 714, 832
MroXI GAANNNNTTC 1 cut(s) 541
MseI TTAA 2 cut(s) 882, 1029
MslI CAYNNNNRTG 4 cut(s) 375, 471, 725, 914
MspI CCGG 3 cut(s) 655, 715, 833
MspR9I CCNGG 1 cut(s) 656
Mva1269I GAATGC 1 cut(s) 790
MwoI GCNNNNNNNGC 2 cut(s) 163, 493
NciI CCSGG 1 cut(s) 656
NcoI CCATGG 1 cut(s) 984
NdeII GATC 6 cut(s) 223, 373, 379, 442, 789, 906
NheI GCTAGC 1 cut(s) 1064
NlaIV GGNNCC 1 cut(s) 653
NmuCI GTSAC 1 cut(s) 14
NsiI ATGCAT 1 cut(s) 503
NspI RCATGY 2 cut(s) 470, 595
PagI TCATGA 3 cut(s) 36, 370, 376
PciI ACATGT 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 1017
PctI GAATGC 1 cut(s) 790
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 3 cut(s) 214, 919, 1046
PkrI GCNGC 3 cut(s) 411, 414, 610
Ple19I CGATCG 1 cut(s) 792
PleI GAGTC 3 cut(s) 21, 33, 628
PpsI GAGTC 3 cut(s) 21, 33, 628
PscI ACATGT 1 cut(s) 466
PspN4I GGNNCC 1 cut(s) 653
PspPI GGNCC 1 cut(s) 850
PvuI CGATCG 1 cut(s) 792
RsaI GTAC 3 cut(s) 132, 532, 584
RsaNI GTAC 3 cut(s) 131, 531, 583
RseI CAYNNNNRTG 4 cut(s) 375, 471, 725, 914
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 2 cut(s) 882, 1029
SatI GCNGC 3 cut(s) 410, 413, 609
Sau3AI GATC 6 cut(s) 223, 373, 379, 442, 789, 906
Sau96I GGNCC 1 cut(s) 850
ScaI AGTACT 3 cut(s) 132, 532, 584
SchI GAGTC 3 cut(s) 22, 34, 628
ScrFI CCNGG 1 cut(s) 656
SduI GDGCHC 1 cut(s) 363
SfaNI GCATC 1 cut(s) 488
SfcI CTRYAG 1 cut(s) 172
SinI GGWCC 1 cut(s) 850
SmiMI CAYNNNNRTG 4 cut(s) 375, 471, 725, 914
SmlI CTYRAG 1 cut(s) 603
SmoI CTYRAG 1 cut(s) 603
Sse9I AATT 4 cut(s) 49, 95, 554, 949
SsiI CCGC 4 cut(s) 155, 453, 862, 1062
SspMI CTAG 2 cut(s) 564, 1065
StyD4I CCNGG 1 cut(s) 654
StyI CCWWGG 1 cut(s) 984
TaaI ACNGT 1 cut(s) 7
TaiI ACGT 1 cut(s) 627
TaqI TCGA 3 cut(s) 535, 792, 1003
TasI AATT 4 cut(s) 49, 95, 554, 949
TatI WGTACW 3 cut(s) 130, 530, 582
TfiI GAWTC 3 cut(s) 214, 919, 1046
Tru1I TTAA 2 cut(s) 882, 1029
Tru9I TTAA 2 cut(s) 882, 1029
TscAI CASTG 2 cut(s) 12, 121
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 3 cut(s) 409, 412, 608
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 8 cut(s) 84, 162, 187, 453, 534, 783, 932, 1044
TspRI CASTG 2 cut(s) 12, 121
VpaK11BI GGWCC 1 cut(s) 850
XceI RCATGY 2 cut(s) 470, 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 135
XmnI GAANNNNTTC 1 cut(s) 541
XspI CTAG 2 cut(s) 564, 1065
ZrmI AGTACT 3 cut(s) 132, 532, 584
Zsp2I ATGCAT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.