AT1G76990

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006284
Physical Location & Seq
Forward (+)
28932119 .. 28936371
4253 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76990.2

Sequence Viewer

Length: 1362 bp
ATGGCTAAAGTTTATTGGCCTTATTTCGATCCTGAATATGAGAACTTGAGCTCCAGAATCAATCCTCCAAGTGTTTCTATAGATAACACTAGCTGCAAAGAATGCACTCTTGTCAAGGTGGACAGTATGAACAAACCTGGAATACTACTTGAAGTTGTGCAAGTCCTAACCGATCTCGATCTCACTATCACTAAAGCTTATATCTCTTCTGATGGTGGATGGTTCATGGACGTATTCCATGTCACCGATCAACAAGGAAACAAGGTTACTGATAGCAAAACCATCGATTACATCGAGAAGGTGTTAGGACCAAAGGGTCATGCTTCGGCTTCACAAAACACTTGGCCTGGTAAAAGAGTCGGTGTCCATTCATTAGGCGACCACACATCGATAGAGATTATTGCTCGTGATCGTCCTGGTCTCTTGTCGGAGGTTTCAGCCGTACTAGCAGACCTCAACATTAATGTGGTGGCAGCTGAAGCATGGACTCACAACCGTAGGATTGCGTGTGTCCTCTATGTGAATGACAATGCAACTTCTAGAGCCGTTGATGATCCAGAAAGATTGTCTTCCATGGAAGAACAGCTTAACAATGTGCTGCGTGGGTGCGAAGAACAAGATGAGAAATTTGCTCGGACGAGTCTCTCCATTGGGTCGACTCACGTTGATCGAAGGCTTCATCAGATGTTTTTCGCTGATAGAGACTACGAAGCAGTGACTAAGCTTGATGATTCTGCTTCTTGCGGATTCGAGCCCAAAATCACGGTTGAGCATTGTGAAGAGAAAGGTTACTCCGTGATAAACGTGAGCTGCGAGGATCGACCAAAGCTCATGTTTGACATTGTATGCACGCTTACGGATATGCAATACATTGTGTTTCACGCCACGATTTCATCAAGCGGCTCTCATGCTTCTCAGGAGTATTTCATCAGACACAAAGACGGTTGCACTCTTGACACAGAAGGAGAGAAAGAGAGAGTTGTCAAATGTCTGGAAGCTGCAATCCATAGACGAGTCAGCGAGGGTTGGAGTTTGGAGCTCTGCGCAAAGGACAGAGTTGGATTACTGTCGGAAGTGACAAGGATTCTGAGAGAGCACGGGCTATCAGTGTCGAGAGCTGGTGTGACAACAGTAGGAGAACAAGCCGTCAACGTTTTCTATGTGAAAGATGCTTCAGGGAATCCAGTGGATGTGAAGACGATTGAGGCGTTACGCGGAGAGATTGGACACAGTATGATGATTGACTTCAAGAATAAAGTTCCGAGCAGAAAATGGAAAGAAGAAGGTCAAGCCGGAACAGGAGGAGGATGGGCCAAAACCAGTTTCTTCTTTGGGAATTTGCTGGAGAAGTTACTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

453

Amino Acids

50.4

Weight (kDa)

5.46

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 37 - 88 8.3e-09 ACT domain
ACT PF01842 129 - 199 6.5e-11 ACT domain
ACT PF01842 266 - 329 1.8e-08 ACT domain
ACT PF01842 344 - 405 2.2e-10 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 315
Acc16I TGCGCA 1 cut(s) 1045
AccI GTMKAC 1 cut(s) 656
AccII CGCG 1 cut(s) 1215
AciI CCGC 3 cut(s) 744, 900, 1215
AclI AACGTT 1 cut(s) 1152
AclWI GGATC 3 cut(s) 23, 548, 825
AcsI RAATTY 2 cut(s) 626, 1336
AcuI CTGAAG 2 cut(s) 498, 1158
AfaI GTAC 1 cut(s) 444
AgsI TTSAA 2 cut(s) 152, 1249
AjnI CCWGG 3 cut(s) 136, 346, 415
AjuI GAANNNNNNNTTGG 2 cut(s) 1307, 1339
AloI GAACNNNNNNTCC 2 cut(s) 35, 67
Alw21I GWGCWC 3 cut(s) 53, 1041, 1098
Alw26I GTCTC 3 cut(s) 425, 647, 696
AlwI GGATC 3 cut(s) 23, 548, 825
AoxI GGCC 3 cut(s) 17, 344, 1311
ApeKI GCWGC 5 cut(s) 93, 473, 598, 810, 998
ApoI RAATTY 2 cut(s) 626, 1336
AseI ATTAAT 1 cut(s) 462
AspLEI GCGC 1 cut(s) 1046
AspS9I GGNCC 2 cut(s) 308, 1311
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 308
BanII GRGCYC 3 cut(s) 53, 756, 1041
BauI CACGAG 1 cut(s) 405
BbsI GAAGAC 2 cut(s) 561, 1202
Bbv12I GWGCWC 3 cut(s) 53, 1041, 1098
BbvI GCAGC 5 cut(s) 80, 485, 585, 797, 985
BccI CCATC 4 cut(s) 206, 213, 290, 1302
BceAI ACGGC 3 cut(s) 425, 530, 1130
BcgI CGANNNNNNTGC 2 cut(s) 265, 299
BciT130I CCWGG 3 cut(s) 138, 348, 417
BcoDI GTCTC 3 cut(s) 425, 647, 696
BfaI CTAG 3 cut(s) 90, 446, 540
BfmI CTRYAG 1 cut(s) 78
BisI GCNGC 6 cut(s) 94, 474, 599, 811, 901, 999
BlsI GCNGC 6 cut(s) 95, 475, 600, 812, 902, 1000
Bme1390I CCNGG 3 cut(s) 138, 348, 417
Bme18I GGWCC 1 cut(s) 308
BmgT120I GGNCC 2 cut(s) 308, 1311
BmrFI CCNGG 3 cut(s) 138, 348, 417
BmsI GCATC 1 cut(s) 1159
BpiI GAAGAC 2 cut(s) 561, 1202
BpmI CTGGAG 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 67
Bsa29I ATCGAT 2 cut(s) 285, 389
BsaBI GATNNNNATC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 573
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bse1I ACTGG 2 cut(s) 1184, 1320
Bse8I GATNNNNATC 1 cut(s) 177
BseBI CCWGG 3 cut(s) 138, 348, 417
BseCI ATCGAT 2 cut(s) 285, 389
BseDI CCNNGG 1 cut(s) 573
BseGI GGATG 3 cut(s) 224, 1195, 1313
BseJI GATNNNNATC 1 cut(s) 177
BseMII CTCAG 2 cut(s) 929, 1079
BseNI ACTGG 2 cut(s) 1184, 1320
BseRI GAGGAG 1 cut(s) 1317
BseXI GCAGC 5 cut(s) 80, 485, 585, 797, 985
Bsh1236I CGCG 1 cut(s) 1215
BshFI GGCC 3 cut(s) 19, 346, 1313
BshVI ATCGAT 2 cut(s) 285, 389
BsiHKAI GWGCWC 3 cut(s) 53, 1041, 1098
BsiSI CCGG 1 cut(s) 1293
BsmAI GTCTC 3 cut(s) 425, 647, 696
BsmI GAATGC 1 cut(s) 107
BsnI GGCC 3 cut(s) 19, 346, 1313
Bso31I GGTCTC 1 cut(s) 425
Bsp1286I GDGCHC 4 cut(s) 53, 756, 1041, 1098
Bsp143I GATC 8 cut(s) 28, 172, 178, 247, 409, 553, 667, 817
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 3 cut(s) 744, 900, 1215
BspANI GGCC 3 cut(s) 19, 346, 1313
BspCNI CTCAG 2 cut(s) 928, 1080
BspDI ATCGAT 2 cut(s) 285, 389
BspFNI CGCG 1 cut(s) 1215
BspPI GGATC 3 cut(s) 23, 548, 825
BspTNI GGTCTC 1 cut(s) 425
BsrI ACTGG 2 cut(s) 1184, 1320
BssECI CCNNGG 1 cut(s) 573
BssMI GATC 8 cut(s) 28, 172, 178, 247, 409, 553, 667, 817
BssSI CACGAG 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 573
Bst2BI CACGAG 1 cut(s) 405
Bst2UI CCWGG 3 cut(s) 138, 348, 417
Bst4CI ACNGT 7 cut(s) 125, 497, 766, 944, 1068, 1132, 1232
Bst6I CTCTTC 2 cut(s) 211, 774
BstAPI GCANNNNNTGC 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 851
BstDEI CTNAG 3 cut(s) 720, 915, 1088
BstDSI CCRYGG 1 cut(s) 573
BstF5I GGATG 3 cut(s) 224, 1195, 1313
BstFNI CGCG 1 cut(s) 1215
BstHHI GCGC 1 cut(s) 1046
BstKTI GATC 8 cut(s) 31, 175, 181, 250, 412, 556, 670, 820
BstMAI GTCTC 3 cut(s) 425, 647, 696
BstMBI GATC 8 cut(s) 28, 172, 178, 247, 409, 553, 667, 817
BstMWI GCNNNNNNNGC 3 cut(s) 102, 446, 479
BstNI CCWGG 3 cut(s) 138, 348, 417
BstSCI CCNGG 3 cut(s) 136, 346, 415
BstSFI CTRYAG 1 cut(s) 78
BstUI CGCG 1 cut(s) 1215
BstV1I GCAGC 5 cut(s) 80, 485, 585, 797, 985
BstV2I GAAGAC 2 cut(s) 561, 1202
Bsu15I ATCGAT 2 cut(s) 285, 389
BsuRI GGCC 3 cut(s) 19, 346, 1313
BsuTUI ATCGAT 2 cut(s) 285, 389
BtgI CCRYGG 1 cut(s) 573
BtsCI GGATG 3 cut(s) 224, 1195, 1313
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 3 cut(s) 720, 1113, 1191
Cac8I GCNNGC 1 cut(s) 851
CfoI GCGC 1 cut(s) 1046
Cfr13I GGNCC 2 cut(s) 308, 1311
ClaI ATCGAT 2 cut(s) 285, 389
Csp6I GTAC 1 cut(s) 443
CviAII CATG 7 cut(s) 226, 239, 320, 483, 574, 832, 908
CviQI GTAC 1 cut(s) 443
DdeI CTNAG 3 cut(s) 720, 915, 1088
DpnI GATC 8 cut(s) 30, 174, 180, 249, 411, 555, 669, 819
DpnII GATC 8 cut(s) 28, 172, 178, 247, 409, 553, 667, 817
DrdI GACNNNNNNGTC 1 cut(s) 315
DseDI GACNNNNNNGTC 1 cut(s) 315
Eam1104I CTCTTC 2 cut(s) 211, 774
EarI CTCTTC 2 cut(s) 211, 774
Ecl136II GAGCTC 2 cut(s) 51, 1039
Eco130I CCWWGG 1 cut(s) 573
Eco24I GRGCYC 3 cut(s) 53, 756, 1041
Eco31I GGTCTC 1 cut(s) 425
Eco47I GGWCC 1 cut(s) 308
Eco53kI GAGCTC 2 cut(s) 51, 1039
Eco57I CTGAAG 2 cut(s) 498, 1158
EcoICRI GAGCTC 2 cut(s) 51, 1039
EcoRII CCWGG 3 cut(s) 136, 346, 415
EcoT14I CCWWGG 1 cut(s) 573
EcoT38I GRGCYC 3 cut(s) 53, 756, 1041
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 7 cut(s) 229, 242, 323, 486, 577, 835, 911
FalI AAGNNNNNCTT 4 cut(s) 553, 585, 570, 602
FatI CATG 7 cut(s) 225, 238, 319, 482, 573, 831, 907
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 6 cut(s) 94, 474, 599, 811, 901, 999
FokI GGATG 3 cut(s) 231, 1202, 1320
FriOI GRGCYC 3 cut(s) 53, 756, 1041
Fsp4HI GCNGC 6 cut(s) 94, 474, 599, 811, 901, 999
FspBI CTAG 3 cut(s) 90, 446, 540
FspI TGCGCA 1 cut(s) 1045
GlaI GCGC 1 cut(s) 1045
GluI GCNGC 6 cut(s) 94, 474, 599, 811, 901, 999
GsuI CTGGAG 1 cut(s) 37
HaeIII GGCC 3 cut(s) 19, 346, 1313
HapII CCGG 1 cut(s) 1293
HhaI GCGC 1 cut(s) 1046
Hin1II CATG 7 cut(s) 229, 242, 323, 486, 577, 835, 911
Hin6I GCGC 1 cut(s) 1044
HinP1I GCGC 1 cut(s) 1044
HincII GTYRAC 2 cut(s) 657, 1150
HindII GTYRAC 2 cut(s) 657, 1150
HindIII AAGCTT 2 cut(s) 195, 722
HpaII CCGG 1 cut(s) 1293
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 3 cut(s) 121, 657, 1150
Hpy188I TCNGA 8 cut(s) 211, 430, 636, 684, 932, 1072, 1089, 1263
Hpy8I GTNNAC 3 cut(s) 121, 657, 1150
HpyAV CCTTC 4 cut(s) 292, 666, 956, 1277
HpyCH4III ACNGT 7 cut(s) 125, 497, 766, 944, 1068, 1132, 1232
HpyCH4IV ACGT 4 cut(s) 231, 663, 804, 1152
HpyCH4V TGCA 8 cut(s) 96, 105, 160, 533, 849, 865, 948, 1001
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 446, 479
HpyF3I CTNAG 3 cut(s) 720, 915, 1088
HpySE526I ACGT 4 cut(s) 231, 663, 804, 1152
Hsp92II CATG 7 cut(s) 229, 242, 323, 486, 577, 835, 911
HspAI GCGC 1 cut(s) 1044
Kzo9I GATC 8 cut(s) 28, 172, 178, 247, 409, 553, 667, 817
LmnI GCTCC 2 cut(s) 56, 1036
Lsp1109I GCAGC 5 cut(s) 80, 485, 585, 797, 985
LweI GCATC 1 cut(s) 1159
MaeI CTAG 3 cut(s) 90, 446, 540
MaeII ACGT 4 cut(s) 231, 663, 804, 1152
MaeIII GTNAC 8 cut(s) 241, 265, 715, 788, 1075, 1123, 1209, 1350
MalI GATC 8 cut(s) 30, 174, 180, 249, 411, 555, 669, 819
MboI GATC 8 cut(s) 28, 172, 178, 247, 409, 553, 667, 817
MboII GAAGA 8 cut(s) 198, 561, 590, 623, 791, 1207, 1292, 1318
MhlI GDGCHC 4 cut(s) 53, 756, 1041, 1098
MluCI AATT 2 cut(s) 626, 1336
MlyI GAGTC 5 cut(s) 366, 481, 649, 652, 1023
MmeI TCCRAC 4 cut(s) 408, 1007, 1039, 1050
MnlI CCTC 9 cut(s) 75, 424, 464, 524, 808, 1015, 1198, 1295, 1298
MseI TTAA 2 cut(s) 462, 588
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 1 cut(s) 1293
MspR9I CCNGG 3 cut(s) 138, 348, 417
Mva1269I GAATGC 1 cut(s) 107
MvaI CCWGG 3 cut(s) 138, 348, 417
MvnI CGCG 1 cut(s) 1215
MwoI GCNNNNNNNGC 3 cut(s) 102, 446, 479
NcoI CCATGG 1 cut(s) 573
NdeII GATC 8 cut(s) 28, 172, 178, 247, 409, 553, 667, 817
NlaIII CATG 7 cut(s) 229, 242, 323, 486, 577, 835, 911
NmuCI GTSAC 4 cut(s) 241, 715, 1075, 1123
NsbI TGCGCA 1 cut(s) 1045
PcsI WCGNNNNNNNCGW 3 cut(s) 291, 810, 1205
PctI GAATGC 1 cut(s) 107
PfeI GAWTC 5 cut(s) 57, 731, 747, 1084, 1180
PkrI GCNGC 6 cut(s) 95, 475, 600, 812, 902, 1000
PleI GAGTC 5 cut(s) 365, 481, 648, 652, 1022
PpsI GAGTC 5 cut(s) 365, 481, 648, 652, 1022
PshBI ATTAAT 1 cut(s) 462
Psp124BI GAGCTC 2 cut(s) 53, 1041
Psp1406I AACGTT 1 cut(s) 1152
Psp6I CCWGG 3 cut(s) 136, 346, 415
PspGI CCWGG 3 cut(s) 136, 346, 415
PspPI GGNCC 2 cut(s) 308, 1311
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 1 cut(s) 444
RsaNI GTAC 1 cut(s) 443
RseI CAYNNNNRTG 1 cut(s) 464
SacI GAGCTC 2 cut(s) 53, 1041
SalI GTCGAC 1 cut(s) 655
SaqAI TTAA 2 cut(s) 462, 588
SatI GCNGC 6 cut(s) 94, 474, 599, 811, 901, 999
Sau3AI GATC 8 cut(s) 28, 172, 178, 247, 409, 553, 667, 817
Sau96I GGNCC 2 cut(s) 308, 1311
SchI GAGTC 5 cut(s) 366, 481, 649, 652, 1023
ScrFI CCNGG 3 cut(s) 138, 348, 417
SduI GDGCHC 4 cut(s) 53, 756, 1041, 1098
SfaNI GCATC 1 cut(s) 1159
SfcI CTRYAG 1 cut(s) 78
SinI GGWCC 1 cut(s) 308
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 2 cut(s) 626, 1336
SsiI CCGC 3 cut(s) 744, 900, 1215
SspMI CTAG 3 cut(s) 90, 446, 540
SstI GAGCTC 2 cut(s) 53, 1041
StyD4I CCNGG 3 cut(s) 136, 346, 415
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 7 cut(s) 125, 497, 766, 944, 1068, 1132, 1232
TaiI ACGT 4 cut(s) 234, 666, 807, 1155
TasI AATT 2 cut(s) 626, 1336
TauI GCSGC 1 cut(s) 903
TfiI GAWTC 5 cut(s) 57, 731, 747, 1084, 1180
Tru1I TTAA 2 cut(s) 462, 588
Tru9I TTAA 2 cut(s) 462, 588
TscAI CASTG 3 cut(s) 720, 1113, 1191
TseFI GTSAC 4 cut(s) 241, 715, 1075, 1123
TseI GCWGC 5 cut(s) 93, 473, 598, 810, 998
Tsp45I GTSAC 4 cut(s) 241, 715, 1075, 1123
TspDTI ATGAA 6 cut(s) 143, 214, 360, 668, 882, 916
TspGWI ACGGA 2 cut(s) 784, 872
TspRI CASTG 3 cut(s) 720, 1113, 1191
VpaK11BI GGWCC 1 cut(s) 308
VspI ATTAAT 1 cut(s) 462
XapI RAATTY 2 cut(s) 626, 1336
XbaI TCTAGA 1 cut(s) 539
XmiI GTMKAC 1 cut(s) 656
XspI CTAG 3 cut(s) 90, 446, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.