RLG00000013254

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006284
Physical Location & Seq
Forward (+)
28708322 .. 28712187
3866 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013254

Sequence Viewer

Length: 1410 bp
ATGGCAAGAGTTTGTTGGCCATACTTTGATCCCGAGTATGAGAACCTGAGCACAAGAATCAATCCCCCAAGGGTCTCTGTGGACAACTCTAGCTGCAGTGACTGTACCCTAGTCAAGGTGATTTTGGCTTGTAATCATTACAATTATGATTCTATTGGCGTATATAACATTCTGGCAGTCAATATGTTTCACGTCACTGATCAACAAGGAAAACAGATTACAGACAGAAAGACCATTGACCACATCGAGAAGGCTTTAGGACCAAAGGGCCATATCACAGAAGTACTGAAGACTTGGCCGGGAAAAAGGGTCGGAGTGCATTCTGTAGGTGATCACACAGCCATTGAGCTAATTGGAAGAGACCGCCCAGGTCTCTTATCTGAGATCTCCGCTGTCCTTGCTGACCTCCACTTTAACGTAGTTGCTGCTGAAGTATGGACACACAACAGAAGAATAGCCTGTGTTCTTTATGTCAATGATACTACATGCCAACCTGTGGACAATCCATCCAGATTATCTACCATCGAAGAGAGACTCAAGAACATCTTACGCGGGTGCGAGGATGATAACAATGTAGGTCACACCAGTTTCTCTATGGGGTTCACTCATGTGGATAGACGGCTCCACCAGATGTTGTTTGCTGATAGGGATTATGAGGGCCGTGGTGGATTATCAAATGAGGTTGATTTTCCTGCCTGCTTCCAACCCAAGATCACCATTGAGCGTTGGGAAGAAAAGGGATACTCGGTGGTCAGTGTCCGGTGCAAAGATCGTGCAAAACTTATGTTTGACATTGTATGCACACTCACTGATATGGAATATGTTGTTTTCCATGCCACCATCTCGTCTGATGGTCCATATGCATCACAGGAGTATTATATTCGTCACATGGATGGTTGCACCCTTGATACTGAAGGCGAGAAGGAAAGGGTAGTCAAATGTATTGACGCTGCAATTCGGAGAAGAGTAAGCGAGGGTCTTAGGCTAGAGCTATGTGCAAAGGATAGGGTAGGCTTGCTATCAGAAGTAACAAGGATCCTGAGGGAGAATGGATTATCAGTTATAAGAGCAGGTGTCTCAACCATTGGGGAACAAGGAATGAATGTCTTTTATGTTAGAGATTCTTCAGGGAATTCAGTGGACATACAGAAAATTGAAGCTCTTCGTAAAGAAATTGGACACAAAATGATGTTCAACGTGAAGAAAATTTCAACGAGTTCCAAGGCACCTGAGACACCTGAGACTAGTGGATGGGCCAAGACAAGCTTCTTCTTCGGCAACTTGTTAGAGAGATTTCATTTTGAAATTACTTGGGAAGTTGGGGTTGACATTGTTCAAACTGGAACAAAGGAAATGAGACCAAGAATGGAGCTTTCACATTTCCCATATCACTTTGAGCTTCTCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

470

Amino Acids

53.27

Weight (kDa)

6.12

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 114 - 183 5.7e-10 ACT domain
ACT PF01842 250 - 313 5.7e-06 ACT domain
ACT PF01842 329 - 389 1.1e-09 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1064
AarI CACCTGC 1 cut(s) 1061
Acc36I ACCTGC 1 cut(s) 1061
AccB1I GGYRCC 1 cut(s) 1225
AccB7I CCANNNNNTGG 1 cut(s) 496
AccII CGCG 1 cut(s) 552
AciI CCGC 3 cut(s) 364, 390, 552
AclWI GGATC 3 cut(s) 23, 1030, 1043
AcoI YGGCCR 2 cut(s) 17, 296
AcsI RAATTY 2 cut(s) 1132, 1206
AcuI CTGAAG 4 cut(s) 308, 450, 933, 1110
AfaI GTAC 2 cut(s) 106, 285
AfiI CCNNNNNNNGG 2 cut(s) 115, 496
AgsI TTSAA 5 cut(s) 1157, 1195, 1212, 1304, 1337
AhlI ACTAGT 1 cut(s) 1244
AjiI CACGTC 1 cut(s) 193
AjnI CCWGG 1 cut(s) 367
AjuI GAANNNNNNNTTGG 2 cut(s) 1250, 1282
AleI CACNNNNGTG 1 cut(s) 608
AluBI AGCT 7 cut(s) 93, 349, 991, 1160, 1266, 1372, 1399
AluI AGCT 7 cut(s) 93, 349, 991, 1160, 1266, 1372, 1399
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 8 cut(s) 79, 354, 377, 526, 1081, 1226, 1235, 1351
AlwI GGATC 3 cut(s) 23, 1030, 1043
AlwNI CAGNNNCTG 1 cut(s) 102
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 5 cut(s) 17, 268, 296, 658, 1254
ApeKI GCWGC 3 cut(s) 93, 425, 950
ApoI RAATTY 2 cut(s) 1132, 1206
Asp700I GAANNNNTTC 1 cut(s) 1161
AspS9I GGNCC 5 cut(s) 260, 268, 658, 854, 1254
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 3 cut(s) 130, 341, 706
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 2 cut(s) 260, 854
AxyI CCTNAGG 1 cut(s) 1040
BalI TGGCCA 1 cut(s) 19
BamHI GGATCC 1 cut(s) 1035
BanI GGYRCC 1 cut(s) 1225
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 80, 412, 937
BccI CCATC 6 cut(s) 514, 530, 845, 848, 887, 1245
BceAI ACGGC 2 cut(s) 635, 645
BciT130I CCWGG 1 cut(s) 369
BciVI GTATCC 1 cut(s) 734
BclI TGATCA 2 cut(s) 199, 331
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 8 cut(s) 79, 354, 377, 526, 1081, 1226, 1235, 1351
BcuI ACTAGT 1 cut(s) 1244
BfaI CTAG 4 cut(s) 90, 110, 986, 1245
BfmI CTRYAG 2 cut(s) 94, 324
BfuAI ACCTGC 1 cut(s) 1061
BfuI GTATCC 1 cut(s) 734
BglII AGATCT 1 cut(s) 384
BisI GCNGC 3 cut(s) 94, 426, 951
BlsI GCNGC 3 cut(s) 95, 427, 952
BmcAI AGTACT 1 cut(s) 285
Bme1390I CCNGG 2 cut(s) 300, 369
Bme18I GGWCC 2 cut(s) 260, 854
BmeT110I CYCGRG 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 5 cut(s) 260, 268, 658, 854, 1254
BmiI GGNNCC 3 cut(s) 623, 1037, 1227
BmrFI CCNGG 2 cut(s) 300, 369
BmsI GCATC 1 cut(s) 872
BpiI GAAGAC 1 cut(s) 296
Bpu10I CCTNAGC 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 521
BpuMI CCSGG 1 cut(s) 300
BsaI GGTCTC 4 cut(s) 79, 354, 377, 1351
BsaJI CCNNGG 4 cut(s) 68, 367, 661, 1221
BsaWI WCCGGW 1 cut(s) 759
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 496
Bse1I ACTGG 2 cut(s) 585, 1345
Bse21I CCTNAGG 1 cut(s) 1040
BseBI CCWGG 1 cut(s) 369
BseDI CCNNGG 4 cut(s) 68, 367, 661, 1221
BseGI GGATG 4 cut(s) 506, 568, 898, 1256
BseLI CCNNNNNNNGG 2 cut(s) 115, 496
BseMII CTCAG 5 cut(s) 38, 372, 1031, 1221, 1230
BseNI ACTGG 2 cut(s) 585, 1345
BseXI GCAGC 3 cut(s) 80, 412, 937
Bsh1236I CGCG 1 cut(s) 552
BshFI GGCC 5 cut(s) 19, 270, 298, 660, 1256
BshNI GGYRCC 1 cut(s) 1225
BsiHKAI GWGCWC 1 cut(s) 53
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 2 cut(s) 299, 760
BslI CCNNNNNNNGG 2 cut(s) 115, 496
BsmAI GTCTC 8 cut(s) 79, 354, 377, 526, 1081, 1226, 1235, 1351
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 5 cut(s) 19, 270, 298, 660, 1256
Bso31I GGTCTC 4 cut(s) 79, 354, 377, 1351
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 7 cut(s) 28, 199, 331, 384, 711, 769, 1035
BspACI CCGC 3 cut(s) 364, 390, 552
BspANI GGCC 5 cut(s) 19, 270, 298, 660, 1256
BspCNI CTCAG 5 cut(s) 39, 373, 1032, 1222, 1231
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 3 cut(s) 623, 1037, 1227
BspMAI CTGCAG 1 cut(s) 98
BspMI ACCTGC 1 cut(s) 1061
BspPI GGATC 3 cut(s) 23, 1030, 1043
BspQI GCTCTTC 1 cut(s) 1167
BspT107I GGYRCC 1 cut(s) 1225
BspTNI GGTCTC 4 cut(s) 79, 354, 377, 1351
BsrI ACTGG 2 cut(s) 585, 1345
BssECI CCNNGG 4 cut(s) 68, 367, 661, 1221
BssMI GATC 7 cut(s) 28, 199, 331, 384, 711, 769, 1035
BssT1I CCWWGG 2 cut(s) 68, 1221
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 1 cut(s) 104
Bst6I CTCTTC 4 cut(s) 352, 522, 958, 1167
BstC8I GCNNGC 2 cut(s) 697, 1016
BstDEI CTNAG 6 cut(s) 47, 381, 980, 1040, 1230, 1239
BstDSI CCRYGG 1 cut(s) 661
BstENI CCTNNNNNAGG 1 cut(s) 113
BstF5I GGATG 4 cut(s) 506, 568, 898, 1256
BstFNI CGCG 1 cut(s) 552
BstKTI GATC 7 cut(s) 31, 202, 334, 387, 714, 772, 1038
BstMAI GTCTC 8 cut(s) 79, 354, 377, 526, 1081, 1226, 1235, 1351
BstMBI GATC 7 cut(s) 28, 199, 331, 384, 711, 769, 1035
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNI CCWGG 1 cut(s) 369
BstNSI RCATGY 1 cut(s) 489
BstSCI CCNGG 2 cut(s) 298, 367
BstSFI CTRYAG 2 cut(s) 94, 324
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 3 cut(s) 80, 412, 937
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 2 cut(s) 384, 1035
BstYI RGATCY 2 cut(s) 384, 1035
Bsu36I CCTNAGG 1 cut(s) 1040
BsuI GTATCC 1 cut(s) 734
BsuRI GGCC 5 cut(s) 19, 270, 298, 660, 1256
BtgI CCRYGG 1 cut(s) 661
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 4 cut(s) 506, 568, 898, 1256
BtsI GCAGTG 1 cut(s) 103
BtsIMutI CAGTG 5 cut(s) 103, 195, 760, 807, 1143
BveI ACCTGC 1 cut(s) 1061
Cac8I GCNNGC 2 cut(s) 697, 1016
CaiI CAGNNNCTG 1 cut(s) 102
Cfr13I GGNCC 5 cut(s) 260, 268, 658, 854, 1254
CseI GACGC 1 cut(s) 956
Csp6I GTAC 2 cut(s) 105, 284
CviAII CATG 4 cut(s) 486, 608, 833, 889
CviQI GTAC 2 cut(s) 105, 284
DdeI CTNAG 6 cut(s) 47, 381, 980, 1040, 1230, 1239
DpnI GATC 7 cut(s) 30, 201, 333, 386, 713, 771, 1037
DpnII GATC 7 cut(s) 28, 199, 331, 384, 711, 769, 1035
EaeI YGGCCR 2 cut(s) 17, 296
Eam1104I CTCTTC 4 cut(s) 352, 522, 958, 1167
EarI CTCTTC 4 cut(s) 352, 522, 958, 1167
Eco130I CCWWGG 2 cut(s) 68, 1221
Eco31I GGTCTC 4 cut(s) 79, 354, 377, 1351
Eco47I GGWCC 2 cut(s) 260, 854
Eco57I CTGAAG 4 cut(s) 308, 450, 933, 1110
Eco81I CCTNAGG 1 cut(s) 1040
Eco88I CYCGRG 1 cut(s) 32
EcoNI CCTNNNNNAGG 1 cut(s) 113
EcoRI GAATTC 1 cut(s) 1132
EcoRII CCWGG 1 cut(s) 367
EcoT14I CCWWGG 2 cut(s) 68, 1221
EcoT22I ATGCAT 1 cut(s) 865
ErhI CCWWGG 2 cut(s) 68, 1221
FaeI CATG 4 cut(s) 489, 611, 836, 892
FalI AAGNNNNNCTT 4 cut(s) 530, 562, 1250, 1282
FatI CATG 4 cut(s) 485, 607, 832, 888
FauI CCCGC 1 cut(s) 545
FauNDI CATATG 1 cut(s) 859
FbaI TGATCA 2 cut(s) 199, 331
Fnu4HI GCNGC 3 cut(s) 94, 426, 951
FokI GGATG 4 cut(s) 493, 575, 905, 1263
Fsp4HI GCNGC 3 cut(s) 94, 426, 951
FspBI CTAG 4 cut(s) 90, 110, 986, 1245
GluI GCNGC 3 cut(s) 94, 426, 951
HaeIII GGCC 5 cut(s) 19, 270, 298, 660, 1256
HapII CCGG 2 cut(s) 299, 760
HgaI GACGC 1 cut(s) 956
Hin1II CATG 4 cut(s) 489, 611, 836, 892
HincII GTYRAC 1 cut(s) 1327
HindII GTYRAC 1 cut(s) 1327
HindIII AAGCTT 1 cut(s) 1264
HinfI GANTC 4 cut(s) 57, 149, 534, 1121
HpaII CCGG 2 cut(s) 299, 760
HphI GGTGA 3 cut(s) 130, 341, 706
Hpy166II GTNNAC 5 cut(s) 82, 499, 603, 1141, 1327
Hpy188I TCNGA 5 cut(s) 314, 382, 850, 960, 1024
Hpy188III TCNNGA 6 cut(s) 32, 247, 510, 538, 1039, 1403
Hpy8I GTNNAC 5 cut(s) 82, 499, 603, 1141, 1327
HpyAV CCTTC 3 cut(s) 244, 908, 916
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 3 cut(s) 192, 417, 1197
HpyCH4V TGCA 9 cut(s) 96, 319, 765, 776, 801, 863, 900, 953, 998
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 6 cut(s) 47, 381, 980, 1040, 1230, 1239
HpySE526I ACGT 3 cut(s) 192, 417, 1197
Hsp92II CATG 4 cut(s) 489, 611, 836, 892
Ksp22I TGATCA 2 cut(s) 199, 331
Kzo9I GATC 7 cut(s) 28, 199, 331, 384, 711, 769, 1035
LguI GCTCTTC 1 cut(s) 1167
LmnI GCTCC 2 cut(s) 627, 1369
Lsp1109I GCAGC 3 cut(s) 80, 412, 937
LweI GCATC 1 cut(s) 872
MaeI CTAG 4 cut(s) 90, 110, 986, 1245
MaeII ACGT 3 cut(s) 192, 417, 1197
MaeIII GTNAC 5 cut(s) 98, 193, 578, 884, 1027
MalI GATC 7 cut(s) 30, 201, 333, 386, 713, 771, 1037
MboI GATC 7 cut(s) 28, 199, 331, 384, 711, 769, 1035
MflI RGATCY 2 cut(s) 384, 1035
MhlI GDGCHC 1 cut(s) 53
MlsI TGGCCA 1 cut(s) 19
MluCI AATT 8 cut(s) 142, 351, 954, 1132, 1152, 1173, 1206, 1305
MluNI TGGCCA 1 cut(s) 19
MlyI GAGTC 1 cut(s) 528
MmeI TCCRAC 2 cut(s) 292, 727
MnlI CCTC 6 cut(s) 416, 553, 649, 673, 967, 1035
Mox20I TGGCCA 1 cut(s) 19
Mph1103I ATGCAT 1 cut(s) 865
MroXI GAANNNNTTC 1 cut(s) 1161
MscI TGGCCA 1 cut(s) 19
MseI TTAA 2 cut(s) 414, 1408
MslI CAYNNNNRTG 3 cut(s) 608, 812, 891
Msp20I TGGCCA 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 2 cut(s) 299, 760
MspR9I CCNGG 2 cut(s) 300, 369
Mva1269I GAATGC 1 cut(s) 319
MvaI CCWGG 1 cut(s) 369
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 1 cut(s) 398
NciI CCSGG 1 cut(s) 300
NdeI CATATG 1 cut(s) 859
NdeII GATC 7 cut(s) 28, 199, 331, 384, 711, 769, 1035
NlaIII CATG 4 cut(s) 489, 611, 836, 892
NlaIV GGNNCC 3 cut(s) 623, 1037, 1227
NmuCI GTSAC 4 cut(s) 98, 193, 578, 884
NsiI ATGCAT 1 cut(s) 865
NspI RCATGY 1 cut(s) 489
OliI CACNNNNGTG 1 cut(s) 608
PaqCI CACCTGC 1 cut(s) 1061
PciSI GCTCTTC 1 cut(s) 1167
PctI GAATGC 1 cut(s) 319
PdmI GAANNNNTTC 1 cut(s) 1161
PfeI GAWTC 3 cut(s) 57, 149, 1121
PflMI CCANNNNNTGG 1 cut(s) 496
PkrI GCNGC 3 cut(s) 95, 427, 952
PleI GAGTC 1 cut(s) 528
PpsI GAGTC 1 cut(s) 528
PsiI TTATAA 1 cut(s) 1064
Psp6I CCWGG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 367
PspN4I GGNNCC 3 cut(s) 623, 1037, 1227
PspPI GGNCC 5 cut(s) 260, 268, 658, 854, 1254
PstI CTGCAG 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 102
PsuI RGATCY 2 cut(s) 384, 1035
RsaI GTAC 2 cut(s) 106, 285
RsaNI GTAC 2 cut(s) 105, 284
RseI CAYNNNNRTG 3 cut(s) 608, 812, 891
SapI GCTCTTC 1 cut(s) 1167
SaqAI TTAA 2 cut(s) 414, 1408
SatI GCNGC 3 cut(s) 94, 426, 951
Sau3AI GATC 7 cut(s) 28, 199, 331, 384, 711, 769, 1035
Sau96I GGNCC 5 cut(s) 260, 268, 658, 854, 1254
ScaI AGTACT 1 cut(s) 285
SchI GAGTC 1 cut(s) 528
ScrFI CCNGG 2 cut(s) 300, 369
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 1 cut(s) 872
SfcI CTRYAG 2 cut(s) 94, 324
SinI GGWCC 2 cut(s) 260, 854
SmiMI CAYNNNNRTG 3 cut(s) 608, 812, 891
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
SpeI ACTAGT 1 cut(s) 1244
Sse9I AATT 8 cut(s) 142, 351, 954, 1132, 1152, 1173, 1206, 1305
SsiI CCGC 3 cut(s) 364, 390, 552
SspMI CTAG 4 cut(s) 90, 110, 986, 1245
StyD4I CCNGG 2 cut(s) 298, 367
StyI CCWWGG 2 cut(s) 68, 1221
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 3 cut(s) 195, 420, 1200
TaqI TCGA 3 cut(s) 246, 525, 1404
TasI AATT 8 cut(s) 142, 351, 954, 1132, 1152, 1173, 1206, 1305
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 3 cut(s) 57, 149, 1121
Tru1I TTAA 2 cut(s) 414, 1408
Tru9I TTAA 2 cut(s) 414, 1408
TscAI CASTG 5 cut(s) 103, 202, 760, 814, 1143
TseFI GTSAC 4 cut(s) 98, 193, 578, 884
TseI GCWGC 3 cut(s) 93, 425, 950
Tsp45I GTSAC 4 cut(s) 98, 193, 578, 884
TspDTI ATGAA 2 cut(s) 1115, 1286
TspRI CASTG 5 cut(s) 103, 202, 760, 814, 1143
Van91I CCANNNNNTGG 1 cut(s) 496
VpaK11BI GGWCC 2 cut(s) 260, 854
XagI CCTNNNNNAGG 1 cut(s) 113
XapI RAATTY 2 cut(s) 1132, 1206
XceI RCATGY 1 cut(s) 489
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XmnI GAANNNNTTC 1 cut(s) 1161
XspI CTAG 4 cut(s) 90, 110, 986, 1245
ZrmI AGTACT 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.