Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006284

Physical Location & Seq

Genomic Coordinates Forward (+)

2095677 .. 2098094

Gene Structure 2418 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1010300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1122 bp

ATGTTATTTCACGTCACGGATCAGCAAGGAAAAAAAGTTACAGACAGCAAAACCATTGATTACATAGAGAAGGCTTTGGGACCAAAGGGCCATATCACAGACGTGCTGAATGCTTGGCCAGGAAAACGGGTTGGAGTACACTCTTTCGGGGATCATACAGCCATTGAGCTTATTGGCAAAGACCGACCAGGTCTCTTATCTGAGATCTCTGCTGTCCTTGCCAACCTTCACTTTAATGTAATTGCTGCCGAAGTATGGACACACAATAGACGAATAGCTTGCGTTGTGTATGTCAATGATAATACTGCATGCCAACCTGTGGAGGATAAAGCCCGATTATCTACTATGGAGGAGCAGCTCAAGAACATCCTACGCGGGTGTGAGGACGATGAGAAAGTTGGTCGCACTAGCTTCTCTATGGGATTCACCCATGTTGATAGGCGGCTCCACCAGATGTTGTTTGCTGATAGGGATTATGAGGGTGGTGGACTGGCAAATGAAGTTGATCACTTTCCTCCCTGCTTCCAACCAAAGATCACCATTGAGCGTTGTGAAGAAAAAGGGTACTCGGTGGTTAGTGTGCGGTGCAAAGACCGTGCAAAGCTCATGTTTGACATCGTATGCACACTCACAGATATGCAGTACGTTGTTTTCCATGCCTCCATTTCATCTGATGGCCCATACGCATCACAGGAGTATTTTATTCGTCACATGGATGGTTGCACCCTCGATACTGAAGGAGAGAAGGAAAGGGTCGTCAAATGTATTGACGCTGCAATTCGGAGAAGAGTAAGCGAGGGTCTAAGGCTAGAACTGTGTGCAAAGGATAGAGTAGGCTTGCTATCTGAAGTAACAAGAATTCTGAGGGAGAATGGATTGTCAGTTACAAGGGCTGGCGTCACAACAATTGGGGAGCAAGCAATGAATATCTTTTATGTTAGAGATTCTTCAGAGAATCCCGTGGACATAAAAACAATAGCAGCACTTCGTAAAGAAATCGGGCATACAATGATGTTCAATGTGAAGAAAGTCCCAACAAATTCTGCTGAGGCACCTGAGACTAAGGGATGGGCTAAGACAAGCTTCTTCTTTGGGAACTTGTTAGAGAGATTCTTGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACT

Protein Analysis

374

Amino Acids

41.91

Weight (kDa)

6.47

Isoelectric Point (pI)

37.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT	PF01842	54 - 101	2.1e-08	ACT domain
ACT	PF01842	191 - 254	6.7e-07	ACT domain
ACT	PF01842	270 - 329	1.4e-09	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006284)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76990 AT1G76990 AT1G76990 AT1G76990 AT1G76990
fragaria_vesca	FvH4_2g15200
malus_domestica	MD05G1010200.v1.1 MD05G1010300.v1.1 MD10G1008800.v1.1
prunus_persica	Prupe.8G012600_v2.0.a1 Prupe.8G012600_v2.0.a1 Prupe.8G012600_v2.0.a1 Prupe.8G012600_v2.0.a1 Prupe.8G012600_v2.0.a1
pyrus_communis	pycom05g00530 pycom10g00530
rosa_chinensis	RchiOBHm_Chr6g0277951
rosa_laevigata	RLG00000013254
rosa_multiflora	Rmu_sc0001474.1_g000003
rosa_roxburghii	Rroxscaffold_1G00047070 Rroxscaffold_7G00190650
rosa_rugosa	Rorug06G0113000
rosa_samantha	Rh6CG230000 Rh6DG220700 Rh6DG220800
rosa_wichuraiana	Rw5G024040 Rw6G019510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1051
AccB7I	CCANNNNNTGG	1 cut(s)	319
AccII	CGCG	1 cut(s)	375
AciI	CCGC	3 cut(s)	375, 442, 583
AclWI	GGATC	2 cut(s)	27, 159
AcoI	YGGCCR	1 cut(s)	116
AcsI	RAATTY	2 cut(s)	858, 1039
AcuI	CTGAAG	3 cut(s)	756, 867, 933
AcyI	GRCGYC	1 cut(s)	897
AfaI	GTAC	3 cut(s)	138, 566, 644
AfiI	CCNNNNNNNGG	2 cut(s)	255, 319
AgsI	TTSAA	1 cut(s)	1018
AjiI	CACGTC	2 cut(s)	13, 103
AjnI	CCWGG	2 cut(s)	118, 187
AleI	CACNNNNGTG	1 cut(s)	101
AluBI	AGCT	6 cut(s)	169, 278, 358, 411, 604, 1083
AluI	AGCT	6 cut(s)	169, 278, 358, 411, 604, 1083
Alw26I	GTCTC	2 cut(s)	197, 1052
AlwI	GGATC	2 cut(s)	27, 159
AoxI	GGCC	3 cut(s)	88, 116, 676
ApeKI	GCWGC	4 cut(s)	245, 355, 773, 980
ApoI	RAATTY	2 cut(s)	858, 1039
AspS9I	GGNCC	3 cut(s)	80, 88, 677
AsuHPI	GGTGA	2 cut(s)	418, 529
AvaII	GGWCC	1 cut(s)	80
BalI	TGGCCA	1 cut(s)	118
BanI	GGYRCC	1 cut(s)	1051
BbvCI	CCTCAGC	1 cut(s)	1047
BbvI	GCAGC	4 cut(s)	232, 367, 760, 992
BccI	CCATC	3 cut(s)	668, 710, 1062
BcgI	CGANNNNNNTGC	2 cut(s)	261, 295
BciT130I	CCWGG	2 cut(s)	120, 189
BclI	TGATCA	1 cut(s)	505
BcoDI	GTCTC	2 cut(s)	197, 1052
BfaI	CTAG	2 cut(s)	408, 809
BglII	AGATCT	1 cut(s)	204
BisI	GCNGC	5 cut(s)	246, 356, 443, 774, 981
BlsI	GCNGC	5 cut(s)	247, 357, 444, 775, 982
Bme1390I	CCNGG	2 cut(s)	120, 189
Bme18I	GGWCC	1 cut(s)	80
BmgBI	CACGTC	2 cut(s)	13, 103
BmgT120I	GGNCC	3 cut(s)	80, 88, 677
BmiI	GGNNCC	3 cut(s)	81, 446, 1053
BmrFI	CCNGG	2 cut(s)	120, 189
BmsI	GCATC	1 cut(s)	695
Bpu10I	CCTNAGC	1 cut(s)	1047
BpuEI	CTTGAG	1 cut(s)	344
BsaHI	GRCGYC	1 cut(s)	897
BsaI	GGTCTC	1 cut(s)	197
BsaJI	CCNNGG	1 cut(s)	960
Bsc4I	CCNNNNNNNGG	2 cut(s)	255, 319
Bse1I	ACTGG	1 cut(s)	495
Bse3DI	GCAATG	1 cut(s)	927
BseBI	CCWGG	2 cut(s)	120, 189
BseDI	CCNNGG	1 cut(s)	960
BseGI	GGATG	3 cut(s)	366, 721, 1073
BseLI	CCNNNNNNNGG	2 cut(s)	255, 319
BseMI	GCAATG	1 cut(s)	927
BseMII	CTCAG	4 cut(s)	192, 854, 1038, 1047
BseNI	ACTGG	1 cut(s)	495
BseRI	GAGGAG	1 cut(s)	365
BseXI	GCAGC	4 cut(s)	232, 367, 760, 992
Bsh1236I	CGCG	1 cut(s)	375
BshFI	GGCC	3 cut(s)	90, 118, 678
BshNI	GGYRCC	1 cut(s)	1051
BslFI	GGGAC	2 cut(s)	93, 1016
BslI	CCNNNNNNNGG	2 cut(s)	255, 319
BsmAI	GTCTC	2 cut(s)	197, 1052
BsmFI	GGGAC	2 cut(s)	93, 1016
BsmI	GAATGC	1 cut(s)	115
BsnI	GGCC	3 cut(s)	90, 118, 678
Bso31I	GGTCTC	1 cut(s)	197
Bsp143I	GATC	5 cut(s)	19, 151, 204, 505, 534
BspACI	CCGC	3 cut(s)	375, 442, 583
BspANI	GGCC	3 cut(s)	90, 118, 678
BspCNI	CTCAG	4 cut(s)	193, 855, 1039, 1048
BspFNI	CGCG	1 cut(s)	375
BspLI	GGNNCC	3 cut(s)	81, 446, 1053
BspPI	GGATC	2 cut(s)	27, 159
BspT107I	GGYRCC	1 cut(s)	1051
BspTNI	GGTCTC	1 cut(s)	197
BsrDI	GCAATG	1 cut(s)	927
BsrI	ACTGG	1 cut(s)	495
BssECI	CCNNGG	1 cut(s)	960
BssMI	GATC	5 cut(s)	19, 151, 204, 505, 534
BssNI	GRCGYC	1 cut(s)	897
Bst2UI	CCWGG	2 cut(s)	120, 189
Bst4CI	ACNGT	2 cut(s)	596, 816
Bst6I	CTCTTC	1 cut(s)	781
BstACI	GRCGYC	1 cut(s)	897
BstC8I	GCNNGC	5 cut(s)	280, 310, 839, 895, 918
BstDEI	CTNAG	7 cut(s)	201, 803, 863, 1047, 1056, 1062, 1074
BstDSI	CCRYGG	1 cut(s)	960
BstF5I	GGATG	3 cut(s)	366, 721, 1073
BstFNI	CGCG	1 cut(s)	375
BstKTI	GATC	5 cut(s)	22, 154, 207, 508, 537
BstMAI	GTCTC	2 cut(s)	197, 1052
BstMBI	GATC	5 cut(s)	19, 151, 204, 505, 534
BstMWI	GCNNNNNNNGC	1 cut(s)	218
BstNI	CCWGG	2 cut(s)	120, 189
BstNSI	RCATGY	1 cut(s)	312
BstSCI	CCNGG	2 cut(s)	118, 187
BstUI	CGCG	1 cut(s)	375
BstV1I	GCAGC	4 cut(s)	232, 367, 760, 992
BstX2I	RGATCY	1 cut(s)	204
BstYI	RGATCY	1 cut(s)	204
BsuRI	GGCC	3 cut(s)	90, 118, 678
BtgI	CCRYGG	1 cut(s)	960
BtrI	CACGTC	2 cut(s)	13, 103
BtsCI	GGATG	3 cut(s)	366, 721, 1073
Cac8I	GCNNGC	5 cut(s)	280, 310, 839, 895, 918
Cfr13I	GGNCC	3 cut(s)	80, 88, 677
CseI	GACGC	2 cut(s)	779, 886
CsiI	ACCWGGT	1 cut(s)	187
Csp6I	GTAC	3 cut(s)	137, 565, 643
CviAII	CATG	5 cut(s)	309, 431, 607, 656, 712
CviQI	GTAC	3 cut(s)	137, 565, 643
DdeI	CTNAG	7 cut(s)	201, 803, 863, 1047, 1056, 1062, 1074
DpnI	GATC	5 cut(s)	21, 153, 206, 507, 536
DpnII	GATC	5 cut(s)	19, 151, 204, 505, 534
EaeI	YGGCCR	1 cut(s)	116
Eam1104I	CTCTTC	1 cut(s)	781
EarI	CTCTTC	1 cut(s)	781
Eco31I	GGTCTC	1 cut(s)	197
Eco47I	GGWCC	1 cut(s)	80
Eco57I	CTGAAG	3 cut(s)	756, 867, 933
EcoRI	GAATTC	1 cut(s)	858
EcoRII	CCWGG	2 cut(s)	118, 187
FaeI	CATG	5 cut(s)	312, 434, 610, 659, 715
FalI	AAGNNNNNCTT	2 cut(s)	1067, 1099
FaqI	GGGAC	2 cut(s)	93, 1016
FatI	CATG	5 cut(s)	308, 430, 606, 655, 711
FauI	CCCGC	1 cut(s)	368
FbaI	TGATCA	1 cut(s)	505
Fnu4HI	GCNGC	5 cut(s)	246, 356, 443, 774, 981
FokI	GGATG	3 cut(s)	353, 728, 1080
Fsp4HI	GCNGC	5 cut(s)	246, 356, 443, 774, 981
FspBI	CTAG	2 cut(s)	408, 809
GluI	GCNGC	5 cut(s)	246, 356, 443, 774, 981
HaeIII	GGCC	3 cut(s)	90, 118, 678
HgaI	GACGC	2 cut(s)	779, 886
Hin1I	GRCGYC	1 cut(s)	897
Hin1II	CATG	5 cut(s)	312, 434, 610, 659, 715
HindIII	AAGCTT	1 cut(s)	1081
HinfI	GANTC	4 cut(s)	423, 944, 955, 1110
HphI	GGTGA	2 cut(s)	418, 529
Hpy166II	GTNNAC	3 cut(s)	139, 488, 964
Hpy188I	TCNGA	6 cut(s)	202, 673, 783, 847, 864, 952
Hpy188III	TCNNGA	1 cut(s)	361
Hpy8I	GTNNAC	3 cut(s)	139, 488, 964
HpyAV	CCTTC	4 cut(s)	64, 236, 731, 739
HpyCH4III	ACNGT	2 cut(s)	596, 816
HpyCH4IV	ACGT	3 cut(s)	12, 102, 645
HpyCH4V	TGCA	8 cut(s)	308, 588, 599, 624, 640, 723, 776, 821
HpyF10VI	GCNNNNNNNGC	1 cut(s)	218
HpyF3I	CTNAG	7 cut(s)	201, 803, 863, 1047, 1056, 1062, 1074
HpySE526I	ACGT	3 cut(s)	12, 102, 645
Hsp92I	GRCGYC	1 cut(s)	897
Hsp92II	CATG	5 cut(s)	312, 434, 610, 659, 715
Ksp22I	TGATCA	1 cut(s)	505
Kzo9I	GATC	5 cut(s)	19, 151, 204, 505, 534
LmnI	GCTCC	3 cut(s)	352, 450, 913
Lsp1109I	GCAGC	4 cut(s)	232, 367, 760, 992
LweI	GCATC	1 cut(s)	695
MabI	ACCWGGT	1 cut(s)	187
MaeI	CTAG	2 cut(s)	408, 809
MaeII	ACGT	3 cut(s)	12, 102, 645
MaeIII	GTNAC	6 cut(s)	13, 37, 707, 850, 883, 898
MalI	GATC	5 cut(s)	21, 153, 206, 507, 536
MboI	GATC	5 cut(s)	19, 151, 204, 505, 534
MboII	GAAGA	5 cut(s)	566, 798, 939, 1036, 1078
MfeI	CAATTG	1 cut(s)	906
MflI	RGATCY	1 cut(s)	204
MlsI	TGGCCA	1 cut(s)	118
MluCI	AATT	5 cut(s)	240, 777, 858, 906, 1039
MluNI	TGGCCA	1 cut(s)	118
MmeI	TCCRAC	2 cut(s)	112, 550
Mox20I	TGGCCA	1 cut(s)	118
MscI	TGGCCA	1 cut(s)	118
MseI	TTAA	1 cut(s)	234
MslI	CAYNNNNRTG	4 cut(s)	101, 234, 635, 714
Msp20I	TGGCCA	1 cut(s)	118
MspR9I	CCNGG	2 cut(s)	120, 189
MunI	CAATTG	1 cut(s)	906
Mva1269I	GAATGC	1 cut(s)	115
MvaI	CCWGG	2 cut(s)	120, 189
MvnI	CGCG	1 cut(s)	375
MwoI	GCNNNNNNNGC	1 cut(s)	218
NdeII	GATC	5 cut(s)	19, 151, 204, 505, 534
NlaIII	CATG	5 cut(s)	312, 434, 610, 659, 715
NlaIV	GGNNCC	3 cut(s)	81, 446, 1053
NmuCI	GTSAC	3 cut(s)	13, 707, 898
NspI	RCATGY	1 cut(s)	312
OliI	CACNNNNGTG	1 cut(s)	101
PaeI	GCATGC	1 cut(s)	312
PctI	GAATGC	1 cut(s)	115
PfeI	GAWTC	4 cut(s)	423, 944, 955, 1110
PflFI	GACNNNGTC	1 cut(s)	189
PflMI	CCANNNNNTGG	1 cut(s)	319
PkrI	GCNGC	5 cut(s)	247, 357, 444, 775, 982
Psp6I	CCWGG	2 cut(s)	118, 187
PspGI	CCWGG	2 cut(s)	118, 187
PspN4I	GGNNCC	3 cut(s)	81, 446, 1053
PspPI	GGNCC	3 cut(s)	80, 88, 677
PsuI	RGATCY	1 cut(s)	204
PsyI	GACNNNGTC	1 cut(s)	189
RsaI	GTAC	3 cut(s)	138, 566, 644
RsaNI	GTAC	3 cut(s)	137, 565, 643
RseI	CAYNNNNRTG	4 cut(s)	101, 234, 635, 714
SaqAI	TTAA	1 cut(s)	234
SatI	GCNGC	5 cut(s)	246, 356, 443, 774, 981
Sau3AI	GATC	5 cut(s)	19, 151, 204, 505, 534
Sau96I	GGNCC	3 cut(s)	80, 88, 677
ScrFI	CCNGG	2 cut(s)	120, 189
SexAI	ACCWGGT	1 cut(s)	187
SfaNI	GCATC	1 cut(s)	695
SinI	GGWCC	1 cut(s)	80
SmiMI	CAYNNNNRTG	4 cut(s)	101, 234, 635, 714
SmlI	CTYRAG	1 cut(s)	359
SmoI	CTYRAG	1 cut(s)	359
SphI	GCATGC	1 cut(s)	312
Sse9I	AATT	5 cut(s)	240, 777, 858, 906, 1039
SsiI	CCGC	3 cut(s)	375, 442, 583
SspMI	CTAG	2 cut(s)	408, 809
StyD4I	CCNGG	2 cut(s)	118, 187
TaaI	ACNGT	2 cut(s)	596, 816
TaiI	ACGT	3 cut(s)	15, 105, 648
TaqI	TCGA	1 cut(s)	729
TaqII	GACCGA	1 cut(s)	198
TasI	AATT	5 cut(s)	240, 777, 858, 906, 1039
TatI	WGTACW	1 cut(s)	136
TauI	GCSGC	1 cut(s)	445
TfiI	GAWTC	4 cut(s)	423, 944, 955, 1110
Tru1I	TTAA	1 cut(s)	234
Tru9I	TTAA	1 cut(s)	234
TseFI	GTSAC	3 cut(s)	13, 707, 898
TseI	GCWGC	4 cut(s)	245, 355, 773, 980
Tsp45I	GTSAC	3 cut(s)	13, 707, 898
TspDTI	ATGAA	3 cut(s)	513, 657, 938
TspGWI	ACGGA	1 cut(s)	32
Tth111I	GACNNNGTC	1 cut(s)	189
Van91I	CCANNNNNTGG	1 cut(s)	319
VpaK11BI	GGWCC	1 cut(s)	80
XapI	RAATTY	2 cut(s)	858, 1039
XceI	RCATGY	1 cut(s)	312
XspI	CTAG	2 cut(s)	408, 809

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.