MD05G1010200.v1.1

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006284
Physical Location & Seq
Forward (+)
2087276 .. 2087951
676 bp
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UTR
Exon/CDS
Intron
MD05G1010200.v1.1.491

Sequence Viewer

Length: 264 bp
ATGGCTAGAGTTTGTTCGCCATACTTTGATCCTGAGTATGAGAACCTGAGCACAAGAATCAATCCCCCAAGGGTCTCCGTAGACAACTCTAGCTGCAGTGACTGTACTCTAGTCAAGGTTGATAGTGTCAACAAACCGGGAATTCTGCTTGAAGTCGTGCAAATCTTGACAGACCTTGACCTCATAATCACCAAGGCCTATATCTCCTCAGATGGTGGATGGTTCATGGATGGTATGCATATTTTCTGCCTGATTATTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

88

Amino Acids

9.7

Weight (kDa)

4.43

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 37 - 81 2.5e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 81
AclWI GGATC 1 cut(s) 23
AcsI RAATTY 1 cut(s) 141
AfaI GTAC 1 cut(s) 106
AgsI TTSAA 1 cut(s) 152
AluBI AGCT 1 cut(s) 93
AluI AGCT 1 cut(s) 93
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 1 cut(s) 23
AlwNI CAGNNNCTG 1 cut(s) 102
AoxI GGCC 1 cut(s) 195
ApeKI GCWGC 1 cut(s) 93
ApoI RAATTY 1 cut(s) 141
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 80
BccI CCATC 3 cut(s) 206, 213, 224
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 79
BfaI CTAG 3 cut(s) 6, 90, 110
BfmI CTRYAG 1 cut(s) 94
BisI GCNGC 1 cut(s) 94
BlsI GCNGC 1 cut(s) 95
Bme1390I CCNGG 1 cut(s) 138
BmrFI CCNGG 1 cut(s) 138
Bpu10I CCTNAGC 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 79
BsaJI CCNNGG 2 cut(s) 68, 192
BseDI CCNNGG 2 cut(s) 68, 192
BseGI GGATG 2 cut(s) 224, 235
BseMII CTCAG 3 cut(s) 24, 38, 222
BseRI GAGGAG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 80
BshFI GGCC 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 1 cut(s) 137
BsmAI GTCTC 1 cut(s) 79
BsnI GGCC 1 cut(s) 197
Bso31I GGTCTC 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 28
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 3 cut(s) 25, 39, 221
BspMAI CTGCAG 1 cut(s) 98
BspPI GGATC 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 79
BssECI CCNNGG 2 cut(s) 68, 192
BssMI GATC 1 cut(s) 28
BssT1I CCWWGG 2 cut(s) 68, 192
Bst4CI ACNGT 1 cut(s) 104
BstDEI CTNAG 3 cut(s) 33, 47, 208
BstF5I GGATG 2 cut(s) 224, 235
BstKTI GATC 1 cut(s) 31
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 1 cut(s) 94
BstV1I GCAGC 1 cut(s) 80
BsuRI GGCC 1 cut(s) 197
BtsCI GGATG 2 cut(s) 224, 235
BtsI GCAGTG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 103
CaiI CAGNNNCTG 1 cut(s) 102
Csp6I GTAC 1 cut(s) 105
CviAII CATG 1 cut(s) 226
CviJI RGCY 3 cut(s) 5, 93, 197
CviKI_1 RGCY 3 cut(s) 5, 93, 197
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 3 cut(s) 33, 47, 208
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
Eco130I CCWWGG 2 cut(s) 68, 192
Eco147I AGGCCT 1 cut(s) 197
Eco31I GGTCTC 1 cut(s) 79
EcoRI GAATTC 1 cut(s) 141
EcoT14I CCWWGG 2 cut(s) 68, 192
EcoT22I ATGCAT 1 cut(s) 240
ErhI CCWWGG 2 cut(s) 68, 192
FaeI CATG 1 cut(s) 229
FaiI YATR 7 cut(s) 22, 39, 185, 201, 227, 236, 240
FatI CATG 1 cut(s) 225
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 1 cut(s) 94
FokI GGATG 2 cut(s) 231, 242
Fsp4HI GCNGC 1 cut(s) 94
FspBI CTAG 3 cut(s) 6, 90, 110
GluI GCNGC 1 cut(s) 94
HaeIII GGCC 1 cut(s) 197
HapII CCGG 1 cut(s) 137
Hin1II CATG 1 cut(s) 229
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 1 cut(s) 57
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 2 cut(s) 82, 130
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 2 cut(s) 32, 166
Hpy8I GTNNAC 2 cut(s) 82, 130
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4V TGCA 3 cut(s) 96, 160, 238
HpyF3I CTNAG 3 cut(s) 33, 47, 208
Hsp92II CATG 1 cut(s) 229
Kzo9I GATC 1 cut(s) 28
LpnPI CCDG 3 cut(s) 45, 59, 150
Lsp1109I GCAGC 1 cut(s) 80
MaeI CTAG 3 cut(s) 6, 90, 110
MaeIII GTNAC 1 cut(s) 98
MalI GATC 1 cut(s) 30
MboI GATC 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 1 cut(s) 141
MnlI CCTC 2 cut(s) 191, 217
Mph1103I ATGCAT 1 cut(s) 240
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 1 cut(s) 138
NciI CCSGG 1 cut(s) 138
NdeII GATC 1 cut(s) 28
NlaIII CATG 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 98
NsiI ATGCAT 1 cut(s) 240
PceI AGGCCT 1 cut(s) 197
PfeI GAWTC 1 cut(s) 57
PkrI GCNGC 1 cut(s) 95
PstI CTGCAG 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 102
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
SatI GCNGC 1 cut(s) 94
Sau3AI GATC 1 cut(s) 28
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 53
SetI ASST 5 cut(s) 48, 95, 120, 177, 183
SfcI CTRYAG 1 cut(s) 94
Sse9I AATT 1 cut(s) 141
SseBI AGGCCT 1 cut(s) 197
SspMI CTAG 3 cut(s) 6, 90, 110
StuI AGGCCT 1 cut(s) 197
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 2 cut(s) 68, 192
TaaI ACNGT 1 cut(s) 104
TasI AATT 1 cut(s) 141
TatI WGTACW 1 cut(s) 104
TfiI GAWTC 1 cut(s) 57
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 1 cut(s) 93
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 1 cut(s) 214
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 1 cut(s) 103
XapI RAATTY 1 cut(s) 141
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 3 cut(s) 6, 90, 110
Zsp2I ATGCAT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.