FvH4_2g15200

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006284
Physical Location & Seq
Reverse (-)
13371428 .. 13382397
10970 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15200.t1

Sequence Viewer

Length: 1377 bp
ATGGCAAGAGTTTGTTCGCCATACTTTGATCCTGAGTATGAAAACCTGAGCACAAGAATCAATCCTCCAAGGGTCTGTGTGGACAACTCGAGCTGCAGTGACTGTACCCTAGTCAAGGTTGATAGTGTCAATAAGCCGGGAATTCTGCTTGAAGTTGTTCAAGTACTGACAGACCTTGACCTCATAATCACTAAAGCCTATATCTCCTCTGATGGTGGATGGTTCATGGACGTGTTTCACGTCACCGATCAACAAGGAAAGCAGATTACGGACAGAAAAACCATTGATCACATTGAGAAGGCTTTAGGACCAAAGGGGGATACCACAGAAGTAGTGAAGACTTGGCCAGGAAAAAGGGTTGGAGTGCATTCTGTAGGTGATCACACAGCCATTGAGCTAATTGGAAGAGACCGTCCAGGTCTCTTATCTGAGATCTCTGCTGTTCTTGCCGATCTGCACTTTAACGTAGTTGCTGCTGAAGTATGGACACACAACAGAAGAATAGCCTGTGTTCTTTATGTTAACGATACCACATCCCAGCCTGTGGACCATCCAACCAGATTATCGACTATTGAAGAGAGACTCAAGAACATCTTACGTGGGTGCGAAGATGATGACAATGTAGGTCACACAAGTTTCTCTATGGGGTTCACTCATGTGGATAGACGGCTCCACCAGATGTTGTTTGCTGACAGAGATTATGAGGGCAGTGGCGGCCATGGCGGTGGCGGTGGTGGTGGATTATCAAATGAGGTTGATTTTCCTGCCTGTTTCCAACCAAAGATCACCATTGAGCGTTGGGAAGAAAAGGGATACTCGGTGGTCAGTGTCCGGTGCAAAGATCGTGCAAAACTTATGTTTGACATCGTATGCACACTCACTGATATGGAATATGTTGTTTTCCATGCCACCATCTCGTCTGATGGTCCATATGCATCACAGGAGTATTATATTCGTCACATGGATGGTTGCACCCTTGATACCGAAGGTGAGAAGGAAAGGGTAGTCAAATGTATTGATGCTGCAATTCGTAGAAGAGTAAGCGAGGGGCTTAGGCTAGAGCTATGTGCAAAGGATAGGGTAGGCCTGCTATCAGAAGTAACAAGGATCCTGAGGGAGAATGGGTTATCAGTTATAAGAGCAGGCGTCTCAACCATTGGTGAACAAGCAATGAATGTATTTTATGTTAGAGATTCTTCAGGGAACTCAGTGGACATACAAAAAATCGAAGCTCTTCGTAAAGAAATTGGACACACAATGATGTTCAATGTGAAGAAAGTTCCAACGAGTTCTAAGGCACCTGAGACACCTGATACTAGTGGATGGGCAAAGACAAGCTTCTTCTTTGGTAACTTGTTAGAGAGGTTCTTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

459

Amino Acids

50.97

Weight (kDa)

5.74

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 37 - 85 6.3e-08 ACT domain
ACT PF01842 130 - 198 1.9e-09 ACT domain
ACT PF01842 274 - 337 5.5e-06 ACT domain
ACT PF01842 353 - 413 1.1e-09 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1136
AccB1I GGYRCC 1 cut(s) 1297
AccB7I CCANNNNNTGG 1 cut(s) 544
AciI CCGC 3 cut(s) 714, 723, 729
AclWI GGATC 3 cut(s) 23, 1102, 1115
AcoI YGGCCR 2 cut(s) 344, 715
AcsI RAATTY 1 cut(s) 141
AcuI CTGAAG 2 cut(s) 498, 1182
AcyI GRCGYC 1 cut(s) 1146
AfaI GTAC 2 cut(s) 106, 165
AfiI CCNNNNNNNGG 2 cut(s) 115, 544
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 4 cut(s) 152, 161, 575, 1267
AhlI ACTAGT 1 cut(s) 1316
AjiI CACGTC 2 cut(s) 232, 241
AjnI CCWGG 2 cut(s) 346, 415
AleI CACNNNNGTG 1 cut(s) 656
AluBI AGCT 5 cut(s) 93, 397, 1063, 1232, 1338
AluI AGCT 5 cut(s) 93, 397, 1063, 1232, 1338
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 5 cut(s) 402, 425, 574, 1153, 1298
AlwI GGATC 3 cut(s) 23, 1102, 1115
AlwNI CAGNNNCTG 1 cut(s) 102
Ama87I CYCGRG 1 cut(s) 88
AoxI GGCC 3 cut(s) 344, 715, 1084
ApeKI GCWGC 3 cut(s) 93, 473, 1022
ApoI RAATTY 1 cut(s) 141
Asp700I GAANNNNTTC 2 cut(s) 156, 1233
AspS9I GGNCC 3 cut(s) 308, 547, 926
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 5 cut(s) 235, 389, 778, 1001, 1172
AvaI CYCGRG 1 cut(s) 88
AvaII GGWCC 3 cut(s) 308, 547, 926
AxyI CCTNAGG 1 cut(s) 1112
BalI TGGCCA 1 cut(s) 346
BamHI GGATCC 1 cut(s) 1107
BanI GGYRCC 1 cut(s) 1297
BbsI GAAGAC 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 80, 460, 1009
BccI CCATC 7 cut(s) 206, 213, 558, 917, 920, 959, 1317
BceAI ACGGC 1 cut(s) 683
BciT130I CCWGG 2 cut(s) 348, 417
BciVI GTATCC 2 cut(s) 313, 806
BclI TGATCA 2 cut(s) 286, 379
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 5 cut(s) 402, 425, 574, 1153, 1298
BcuI ACTAGT 1 cut(s) 1316
BfaI CTAG 3 cut(s) 110, 1058, 1317
BfmI CTRYAG 2 cut(s) 94, 372
BfuI GTATCC 2 cut(s) 313, 806
BglII AGATCT 1 cut(s) 432
BisI GCNGC 4 cut(s) 94, 474, 715, 1023
BlsI GCNGC 4 cut(s) 95, 475, 716, 1024
BmcAI AGTACT 1 cut(s) 165
Bme1390I CCNGG 3 cut(s) 138, 348, 417
Bme18I GGWCC 3 cut(s) 308, 547, 926
BmeT110I CYCGRG 1 cut(s) 88
BmgBI CACGTC 2 cut(s) 232, 241
BmgT120I GGNCC 3 cut(s) 308, 547, 926
BmiI GGNNCC 3 cut(s) 671, 1109, 1299
BmrFI CCNGG 3 cut(s) 138, 348, 417
BmsI GCATC 2 cut(s) 944, 1009
BpiI GAAGAC 1 cut(s) 344
Bpu10I CCTNAGC 2 cut(s) 47, 1052
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 1 cut(s) 138
BsaAI YACGTR 1 cut(s) 599
BsaHI GRCGYC 1 cut(s) 1146
BsaI GGTCTC 2 cut(s) 402, 425
BsaJI CCNNGG 2 cut(s) 68, 718
BsaWI WCCGGW 1 cut(s) 831
BsaXI ACNNNNNCTCC 2 cut(s) 1109, 1139
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 544
Bse21I CCTNAGG 1 cut(s) 1112
Bse3DI GCAATG 1 cut(s) 1176
BseBI CCWGG 2 cut(s) 348, 417
BseDI CCNNGG 2 cut(s) 68, 718
BseGI GGATG 5 cut(s) 224, 533, 550, 970, 1328
BseLI CCNNNNNNNGG 2 cut(s) 115, 544
BseMI GCAATG 1 cut(s) 1176
BseMII CTCAG 6 cut(s) 24, 38, 420, 1103, 1221, 1293
BseRI GAGGAG 1 cut(s) 196
BseXI GCAGC 3 cut(s) 80, 460, 1009
BseYI CCCAGC 1 cut(s) 537
BsgI GTGCAG 1 cut(s) 440
BshFI GGCC 3 cut(s) 346, 717, 1086
BshNI GGYRCC 1 cut(s) 1297
BsiHKAI GWGCWC 1 cut(s) 53
BsiHKCI CYCGRG 1 cut(s) 88
BsiSI CCGG 2 cut(s) 137, 832
BslI CCNNNNNNNGG 2 cut(s) 115, 544
BsmAI GTCTC 5 cut(s) 402, 425, 574, 1153, 1298
BsmBI CGTCTC 1 cut(s) 1153
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 3 cut(s) 346, 717, 1086
Bso31I GGTCTC 2 cut(s) 402, 425
BsoBI CYCGRG 1 cut(s) 88
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 9 cut(s) 28, 247, 286, 379, 432, 451, 783, 841, 1107
Bsp19I CCATGG 1 cut(s) 718
BspACI CCGC 3 cut(s) 714, 723, 729
BspANI GGCC 3 cut(s) 346, 717, 1086
BspCNI CTCAG 6 cut(s) 25, 39, 421, 1104, 1220, 1294
BspLI GGNNCC 3 cut(s) 671, 1109, 1299
BspMAI CTGCAG 1 cut(s) 98
BspPI GGATC 3 cut(s) 23, 1102, 1115
BspQI GCTCTTC 1 cut(s) 1239
BspT107I GGYRCC 1 cut(s) 1297
BspTNI GGTCTC 2 cut(s) 402, 425
BsrDI GCAATG 1 cut(s) 1176
BssECI CCNNGG 2 cut(s) 68, 718
BssMI GATC 9 cut(s) 28, 247, 286, 379, 432, 451, 783, 841, 1107
BssNI GRCGYC 1 cut(s) 1146
BssT1I CCWWGG 2 cut(s) 68, 718
Bst2UI CCWGG 2 cut(s) 348, 417
Bst4CI ACNGT 2 cut(s) 104, 413
Bst6I CTCTTC 4 cut(s) 400, 570, 1030, 1239
BstACI GRCGYC 1 cut(s) 1146
BstBAI YACGTR 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 1088, 1144
BstDEI CTNAG 8 cut(s) 33, 47, 429, 1052, 1112, 1207, 1293, 1302
BstDSI CCRYGG 1 cut(s) 718
BstENI CCTNNNNNAGG 1 cut(s) 113
BstF5I GGATG 5 cut(s) 224, 533, 550, 970, 1328
BstKTI GATC 9 cut(s) 31, 250, 289, 382, 435, 454, 786, 844, 1110
BstMAI GTCTC 5 cut(s) 402, 425, 574, 1153, 1298
BstMBI GATC 9 cut(s) 28, 247, 286, 379, 432, 451, 783, 841, 1107
BstMWI GCNNNNNNNGC 3 cut(s) 446, 714, 720
BstNI CCWGG 2 cut(s) 348, 417
BstSCI CCNGG 3 cut(s) 136, 346, 415
BstSFI CTRYAG 2 cut(s) 94, 372
BstV1I GCAGC 3 cut(s) 80, 460, 1009
BstV2I GAAGAC 1 cut(s) 344
BstX2I RGATCY 2 cut(s) 432, 1107
BstXI CCANNNNNNTGG 1 cut(s) 725
BstYI RGATCY 2 cut(s) 432, 1107
Bsu36I CCTNAGG 1 cut(s) 1112
BsuI GTATCC 2 cut(s) 313, 806
BsuRI GGCC 3 cut(s) 346, 717, 1086
BtgI CCRYGG 1 cut(s) 718
BtrI CACGTC 2 cut(s) 232, 241
BtsCI GGATG 5 cut(s) 224, 533, 550, 970, 1328
BtsI GCAGTG 2 cut(s) 103, 715
BtsIMutI CAGTG 5 cut(s) 103, 715, 832, 879, 1215
Cac8I GCNNGC 2 cut(s) 1088, 1144
CaiI CAGNNNCTG 1 cut(s) 102
Cfr13I GGNCC 3 cut(s) 308, 547, 926
CseI GACGC 1 cut(s) 1135
Csp6I GTAC 2 cut(s) 105, 164
CviAII CATG 5 cut(s) 226, 656, 719, 905, 961
CviQI GTAC 2 cut(s) 105, 164
DdeI CTNAG 8 cut(s) 33, 47, 429, 1052, 1112, 1207, 1293, 1302
DpnI GATC 9 cut(s) 30, 249, 288, 381, 434, 453, 785, 843, 1109
DpnII GATC 9 cut(s) 28, 247, 286, 379, 432, 451, 783, 841, 1107
EaeI YGGCCR 2 cut(s) 344, 715
Eam1104I CTCTTC 4 cut(s) 400, 570, 1030, 1239
EarI CTCTTC 4 cut(s) 400, 570, 1030, 1239
Eco130I CCWWGG 2 cut(s) 68, 718
Eco147I AGGCCT 1 cut(s) 1086
Eco31I GGTCTC 2 cut(s) 402, 425
Eco47I GGWCC 3 cut(s) 308, 547, 926
Eco57I CTGAAG 2 cut(s) 498, 1182
Eco81I CCTNAGG 1 cut(s) 1112
Eco88I CYCGRG 1 cut(s) 88
EcoNI CCTNNNNNAGG 1 cut(s) 113
EcoRI GAATTC 1 cut(s) 141
EcoRII CCWGG 2 cut(s) 346, 415
EcoT14I CCWWGG 2 cut(s) 68, 718
EcoT22I ATGCAT 1 cut(s) 937
ErhI CCWWGG 2 cut(s) 68, 718
Esp3I CGTCTC 1 cut(s) 1153
FaeI CATG 5 cut(s) 229, 659, 722, 908, 964
FalI AAGNNNNNCTT 4 cut(s) 578, 610, 1322, 1354
FatI CATG 5 cut(s) 225, 655, 718, 904, 960
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 2 cut(s) 286, 379
Fnu4HI GCNGC 4 cut(s) 94, 474, 715, 1023
FokI GGATG 5 cut(s) 231, 520, 537, 977, 1335
Fsp4HI GCNGC 4 cut(s) 94, 474, 715, 1023
FspBI CTAG 3 cut(s) 110, 1058, 1317
GluI GCNGC 4 cut(s) 94, 474, 715, 1023
GsaI CCCAGC 1 cut(s) 541
HaeIII GGCC 3 cut(s) 346, 717, 1086
HapII CCGG 2 cut(s) 137, 832
HgaI GACGC 1 cut(s) 1135
Hin1I GRCGYC 1 cut(s) 1146
Hin1II CATG 5 cut(s) 229, 659, 722, 908, 964
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 1336
HinfI GANTC 3 cut(s) 57, 582, 1193
HpaI GTTAAC 1 cut(s) 523
HpaII CCGG 2 cut(s) 137, 832
HphI GGTGA 5 cut(s) 235, 389, 778, 1001, 1172
Hpy166II GTNNAC 6 cut(s) 82, 523, 547, 651, 1163, 1213
Hpy188I TCNGA 4 cut(s) 211, 430, 922, 1096
Hpy188III TCNNGA 3 cut(s) 32, 586, 1111
Hpy8I GTNNAC 6 cut(s) 82, 523, 547, 651, 1163, 1213
HpyAV CCTTC 3 cut(s) 292, 980, 988
HpyCH4III ACNGT 2 cut(s) 104, 413
HpyCH4IV ACGT 4 cut(s) 231, 240, 465, 598
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 714, 720
HpyF3I CTNAG 8 cut(s) 33, 47, 429, 1052, 1112, 1207, 1293, 1302
HpySE526I ACGT 4 cut(s) 231, 240, 465, 598
Hsp92I GRCGYC 1 cut(s) 1146
Hsp92II CATG 5 cut(s) 229, 659, 722, 908, 964
Ksp22I TGATCA 2 cut(s) 286, 379
KspAI GTTAAC 1 cut(s) 523
Kzo9I GATC 9 cut(s) 28, 247, 286, 379, 432, 451, 783, 841, 1107
LguI GCTCTTC 1 cut(s) 1239
LmnI GCTCC 1 cut(s) 675
Lsp1109I GCAGC 3 cut(s) 80, 460, 1009
LweI GCATC 2 cut(s) 944, 1009
MaeI CTAG 3 cut(s) 110, 1058, 1317
MaeII ACGT 4 cut(s) 231, 240, 465, 598
MaeIII GTNAC 6 cut(s) 98, 241, 626, 956, 1099, 1349
MalI GATC 9 cut(s) 30, 249, 288, 381, 434, 453, 785, 843, 1109
MboI GATC 9 cut(s) 28, 247, 286, 379, 432, 451, 783, 841, 1107
MflI RGATCY 2 cut(s) 432, 1107
MhlI GDGCHC 1 cut(s) 53
MlsI TGGCCA 1 cut(s) 346
MluCI AATT 4 cut(s) 141, 399, 1026, 1245
MluNI TGGCCA 1 cut(s) 346
MlyI GAGTC 1 cut(s) 576
MmeI TCCRAC 4 cut(s) 340, 578, 799, 1307
MnlI CCTC 8 cut(s) 75, 191, 217, 697, 745, 1039, 1107, 1356
Mox20I TGGCCA 1 cut(s) 346
Mph1103I ATGCAT 1 cut(s) 937
MroXI GAANNNNTTC 2 cut(s) 156, 1233
MscI TGGCCA 1 cut(s) 346
MseI TTAA 2 cut(s) 462, 522
MslI CAYNNNNRTG 6 cut(s) 230, 656, 723, 884, 963, 1259
Msp20I TGGCCA 1 cut(s) 346
MspI CCGG 2 cut(s) 137, 832
MspR9I CCNGG 3 cut(s) 138, 348, 417
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 2 cut(s) 348, 417
MwoI GCNNNNNNNGC 3 cut(s) 446, 714, 720
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 1 cut(s) 718
NdeI CATATG 1 cut(s) 931
NdeII GATC 9 cut(s) 28, 247, 286, 379, 432, 451, 783, 841, 1107
NlaIII CATG 5 cut(s) 229, 659, 722, 908, 964
NlaIV GGNNCC 3 cut(s) 671, 1109, 1299
NmuCI GTSAC 4 cut(s) 98, 241, 626, 956
NsiI ATGCAT 1 cut(s) 937
OliI CACNNNNGTG 1 cut(s) 656
PaeR7I CTCGAG 1 cut(s) 88
PceI AGGCCT 1 cut(s) 1086
PciSI GCTCTTC 1 cut(s) 1239
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 1 cut(s) 367
PdmI GAANNNNTTC 2 cut(s) 156, 1233
PfeI GAWTC 2 cut(s) 57, 1193
PflMI CCANNNNNTGG 1 cut(s) 544
PkrI GCNGC 4 cut(s) 95, 475, 716, 1024
PleI GAGTC 1 cut(s) 576
PpsI GAGTC 1 cut(s) 576
Ppu21I YACGTR 1 cut(s) 599
PsiI TTATAA 1 cut(s) 1136
Psp6I CCWGG 2 cut(s) 346, 415
PspFI CCCAGC 1 cut(s) 537
PspGI CCWGG 2 cut(s) 346, 415
PspN4I GGNNCC 3 cut(s) 671, 1109, 1299
PspPI GGNCC 3 cut(s) 308, 547, 926
PspXI VCTCGAGB 1 cut(s) 88
PstI CTGCAG 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 102
PsuI RGATCY 2 cut(s) 432, 1107
RsaI GTAC 2 cut(s) 106, 165
RsaNI GTAC 2 cut(s) 105, 164
RseI CAYNNNNRTG 6 cut(s) 230, 656, 723, 884, 963, 1259
SapI GCTCTTC 1 cut(s) 1239
SaqAI TTAA 2 cut(s) 462, 522
SatI GCNGC 4 cut(s) 94, 474, 715, 1023
Sau3AI GATC 9 cut(s) 28, 247, 286, 379, 432, 451, 783, 841, 1107
Sau96I GGNCC 3 cut(s) 308, 547, 926
ScaI AGTACT 1 cut(s) 165
SchI GAGTC 1 cut(s) 576
ScrFI CCNGG 3 cut(s) 138, 348, 417
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 2 cut(s) 944, 1009
SfcI CTRYAG 2 cut(s) 94, 372
Sfr274I CTCGAG 1 cut(s) 88
SinI GGWCC 3 cut(s) 308, 547, 926
SlaI CTCGAG 1 cut(s) 88
SmiMI CAYNNNNRTG 6 cut(s) 230, 656, 723, 884, 963, 1259
SmlI CTYRAG 2 cut(s) 88, 584
SmoI CTYRAG 2 cut(s) 88, 584
SpeI ACTAGT 1 cut(s) 1316
Sse9I AATT 4 cut(s) 141, 399, 1026, 1245
SseBI AGGCCT 1 cut(s) 1086
SsiI CCGC 3 cut(s) 714, 723, 729
SspMI CTAG 3 cut(s) 110, 1058, 1317
StuI AGGCCT 1 cut(s) 1086
StyD4I CCNGG 3 cut(s) 136, 346, 415
StyI CCWWGG 2 cut(s) 68, 718
TaaI ACNGT 2 cut(s) 104, 413
TaiI ACGT 4 cut(s) 234, 243, 468, 601
TaqI TCGA 3 cut(s) 89, 566, 1227
TasI AATT 4 cut(s) 141, 399, 1026, 1245
TatI WGTACW 1 cut(s) 163
TauI GCSGC 1 cut(s) 717
TfiI GAWTC 2 cut(s) 57, 1193
Tru1I TTAA 2 cut(s) 462, 522
Tru9I TTAA 2 cut(s) 462, 522
TscAI CASTG 5 cut(s) 103, 715, 832, 886, 1215
TseFI GTSAC 4 cut(s) 98, 241, 626, 956
TseI GCWGC 3 cut(s) 93, 473, 1022
Tsp45I GTSAC 4 cut(s) 98, 241, 626, 956
TspDTI ATGAA 3 cut(s) 54, 214, 1187
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 5 cut(s) 103, 715, 832, 886, 1215
Van91I CCANNNNNTGG 1 cut(s) 544
VpaK11BI GGWCC 3 cut(s) 308, 547, 926
XagI CCTNNNNNAGG 1 cut(s) 113
XapI RAATTY 1 cut(s) 141
XhoI CTCGAG 1 cut(s) 88
XmnI GAANNNNTTC 2 cut(s) 156, 1233
XspI CTAG 3 cut(s) 110, 1058, 1317
ZrmI AGTACT 1 cut(s) 165
Zsp2I ATGCAT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.