Rh6DG220800

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0006284
Physical Location & Seq
Reverse (-)
39368260 .. 39397367
29108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG220800.1

Sequence Viewer

Length: 1191 bp
ATGGGGAACGAGAAAAACGGAGCTGGACTCGGAGTTATTTCAACCATTCCAATTCCATCATGTAAAGAATTCTGCTTTTCTTCCTACTACCCTTTTTCAAGTTCAGGATCTGGAGGCCATGTTTGCGTAAGTTGGAAGCTAGCTAGCTGGGTGCATAAAGATAAACAGTCAAATACATGCATCTGGACATTTTTACCAGTTCTCGACTTTTTATTCCCAAGTCTCTTCTGCATCGGCAAGGCGAGGAAGGTACTTCTGTATGAGAACCTGAGCACAAGAATCAATCCCCCAAGGGTCTCTGTGGACAACTCTAGCTGCAGTGCCTGTACCCTAGTCAAGGTTGATAGTGTCAATAAGCCAGGAATTCTGCTTGAAGTTGTGCAAGTACTGACAGACCTTGACCTCATAATCGCTAAAGCCTATATCTCCTCTGATGGCGGATGGTTCATGGATGTGTTTCACGTCACTGATCAACAAGGAAAACAGATTACAGACAGAAAAACCATTGACCATATCGAGAAGGCTTTAGGACCAAAGGGCCACATCACAGAAGTAGTGAAGACTTGGCCAGGAAAAAGGGTTGGAGTGCATTCTGTAGGTGATCACACATCCATTGAGCTAATTGGAAGAGACCGCCCAGGTCTCTTATCTGAGATCTCCGCTGTCCTTGCCGACCTCCACTTTAACGTAGTTGCTGCTGAAGTATGGACACACAACAGAAGAATAGCCTGTGTTCTTTATGTCAATGATGCTACATGCCAACCTGTGGACAATCCATCCAGATTATCTACCATCGAAGAGAGACTCAAGAACATCTTACGTGGGTGCGAGGATGATGACAATGTAGGTCACACCAGTTTCTCTATGGGGTTCACTCATGTGGATAGACGGCTCCACCAGATGTTGTTTGCTGATAGGGATTATGAGGGCCGTGGTGGATTATCAAATGAGGTTGATTTTCCTGCCTGCTTCCAACCCAAGATCACCATTGAGCGTTGGGAAGAAAAGGGATACTCGGTGGTCAGTGTCCGGTGCAAAGATCGTGCAAAACTTATGTTTGACATTGTATGCACACTCACTGATATGGAATATGTTGTTTTCCATGCCACCATCTCGTCTGATGGTCCATATGCATCACAGGTGCAGACATTACTGCATTTTGATTTGCATCATCTTCAATCGTCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

396

Amino Acids

44.16

Weight (kDa)

6.24

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT_ACR9_3rd PF24931 99 - 174 3.8e-06 ACR9-like, ACT-like domain
ACT PF01842 111 - 159 1.2e-07 ACT domain
ACT PF01842 203 - 273 2.2e-10 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 766
AciI CCGC 3 cut(s) 438, 634, 660
AclWI GGATC 1 cut(s) 115
AcoI YGGCCR 1 cut(s) 566
AcsI RAATTY 2 cut(s) 68, 363
AcuI CTGAAG 1 cut(s) 720
AfaI GTAC 3 cut(s) 252, 328, 387
AfiI CCNNNNNNNGG 2 cut(s) 337, 766
AgsI TTSAA 4 cut(s) 42, 99, 374, 1178
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 3 cut(s) 358, 568, 637
AleI CACNNNNGTG 1 cut(s) 878
AluBI AGCT 6 cut(s) 23, 139, 143, 147, 315, 619
AluI AGCT 6 cut(s) 23, 139, 143, 147, 315, 619
Alw21I GWGCWC 1 cut(s) 275
Alw26I GTCTC 5 cut(s) 227, 301, 624, 647, 796
AlwI GGATC 1 cut(s) 115
AlwNI CAGNNNCTG 2 cut(s) 110, 324
AoxI GGCC 4 cut(s) 115, 538, 566, 928
ApeKI GCWGC 2 cut(s) 315, 695
ApoI RAATTY 2 cut(s) 68, 363
AspS9I GGNCC 4 cut(s) 530, 538, 928, 1124
AsuHPI GGTGA 2 cut(s) 611, 976
AsuNHI GCTAGC 2 cut(s) 139, 143
AvaII GGWCC 2 cut(s) 530, 1124
BaeI ACNNNNGTAYC 2 cut(s) 242, 275
BalI TGGCCA 1 cut(s) 568
BbsI GAAGAC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 275
BbvI GCAGC 2 cut(s) 302, 682
BccI CCATC 7 cut(s) 64, 428, 435, 784, 800, 1115, 1118
BceAI ACGGC 2 cut(s) 905, 915
BciT130I CCWGG 3 cut(s) 360, 570, 639
BciVI GTATCC 1 cut(s) 1004
BclI TGATCA 2 cut(s) 469, 601
BcoDI GTCTC 5 cut(s) 227, 301, 624, 647, 796
BfaI CTAG 4 cut(s) 140, 144, 312, 332
BfmI CTRYAG 2 cut(s) 316, 594
BfuI GTATCC 1 cut(s) 1004
BglII AGATCT 1 cut(s) 654
BisI GCNGC 2 cut(s) 316, 696
BlsI GCNGC 2 cut(s) 317, 697
BmcAI AGTACT 1 cut(s) 387
Bme1390I CCNGG 3 cut(s) 360, 570, 639
Bme18I GGWCC 2 cut(s) 530, 1124
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 4 cut(s) 530, 538, 928, 1124
BmiI GGNNCC 1 cut(s) 893
BmrFI CCNGG 3 cut(s) 360, 570, 639
BmsI GCATC 5 cut(s) 189, 240, 739, 1142, 1177
BmtI GCTAGC 2 cut(s) 143, 147
BpiI GAAGAC 1 cut(s) 566
BplI GAGNNNNNCTC 2 cut(s) 12, 44
BpmI CTGGAG 1 cut(s) 132
Bpu10I CCTNAGC 1 cut(s) 269
BpuEI CTTGAG 1 cut(s) 791
BsaAI YACGTR 1 cut(s) 821
BsaBI GATNNNNATC 1 cut(s) 1167
BsaI GGTCTC 3 cut(s) 301, 624, 647
BsaJI CCNNGG 3 cut(s) 290, 637, 931
BsaWI WCCGGW 1 cut(s) 1029
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 2 cut(s) 337, 766
Bse1I ACTGG 2 cut(s) 197, 855
Bse8I GATNNNNATC 1 cut(s) 1167
BseBI CCWGG 3 cut(s) 360, 570, 639
BseDI CCNNGG 3 cut(s) 290, 637, 931
BseGI GGATG 5 cut(s) 446, 457, 608, 776, 838
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 2 cut(s) 337, 766
BseMII CTCAG 2 cut(s) 260, 642
BseNI ACTGG 2 cut(s) 197, 855
BseRI GAGGAG 1 cut(s) 418
BseXI GCAGC 2 cut(s) 302, 682
BseYI CCCAGC 1 cut(s) 147
BsgI GTGCAG 1 cut(s) 1163
BshFI GGCC 4 cut(s) 117, 540, 568, 930
BsiHKAI GWGCWC 1 cut(s) 275
BsiSI CCGG 1 cut(s) 1030
BslI CCNNNNNNNGG 2 cut(s) 337, 766
BsmAI GTCTC 5 cut(s) 227, 301, 624, 647, 796
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 4 cut(s) 117, 540, 568, 930
Bso31I GGTCTC 3 cut(s) 301, 624, 647
Bsp1286I GDGCHC 1 cut(s) 275
Bsp143I GATC 6 cut(s) 107, 469, 601, 654, 981, 1039
BspACI CCGC 3 cut(s) 438, 634, 660
BspANI GGCC 4 cut(s) 117, 540, 568, 930
BspCNI CTCAG 2 cut(s) 261, 643
BspLI GGNNCC 1 cut(s) 893
BspMAI CTGCAG 1 cut(s) 320
BspOI GCTAGC 2 cut(s) 143, 147
BspPI GGATC 1 cut(s) 115
BspTNI GGTCTC 3 cut(s) 301, 624, 647
BsrI ACTGG 2 cut(s) 197, 855
BssECI CCNNGG 3 cut(s) 290, 637, 931
BssMI GATC 6 cut(s) 107, 469, 601, 654, 981, 1039
BssT1I CCWWGG 1 cut(s) 290
Bst2UI CCWGG 3 cut(s) 360, 570, 639
Bst4CI ACNGT 1 cut(s) 168
Bst6I CTCTTC 3 cut(s) 230, 622, 792
BstBAI YACGTR 1 cut(s) 821
BstC8I GCNNGC 3 cut(s) 141, 145, 967
BstDEI CTNAG 2 cut(s) 269, 651
BstDSI CCRYGG 1 cut(s) 931
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 5 cut(s) 446, 457, 608, 776, 838
BstKTI GATC 6 cut(s) 110, 472, 604, 657, 984, 1042
BstMAI GTCTC 5 cut(s) 227, 301, 624, 647, 796
BstMBI GATC 6 cut(s) 107, 469, 601, 654, 981, 1039
BstMWI GCNNNNNNNGC 2 cut(s) 123, 668
BstNI CCWGG 3 cut(s) 360, 570, 639
BstNSI RCATGY 2 cut(s) 180, 759
BstSCI CCNGG 3 cut(s) 358, 568, 637
BstSFI CTRYAG 2 cut(s) 316, 594
BstV1I GCAGC 2 cut(s) 302, 682
BstV2I GAAGAC 1 cut(s) 566
BstX2I RGATCY 2 cut(s) 107, 654
BstYI RGATCY 2 cut(s) 107, 654
BsuI GTATCC 1 cut(s) 1004
BsuRI GGCC 4 cut(s) 117, 540, 568, 930
BtgI CCRYGG 1 cut(s) 931
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 5 cut(s) 446, 457, 608, 776, 838
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 4 cut(s) 325, 465, 1030, 1077
Cac8I GCNNGC 3 cut(s) 141, 145, 967
CaiI CAGNNNCTG 2 cut(s) 110, 324
Cfr13I GGNCC 4 cut(s) 530, 538, 928, 1124
Csp6I GTAC 3 cut(s) 251, 327, 386
CviAII CATG 7 cut(s) 60, 119, 177, 448, 756, 878, 1103
CviQI GTAC 3 cut(s) 251, 327, 386
DdeI CTNAG 2 cut(s) 269, 651
DpnI GATC 6 cut(s) 109, 471, 603, 656, 983, 1041
DpnII GATC 6 cut(s) 107, 469, 601, 654, 981, 1039
EaeI YGGCCR 1 cut(s) 566
Eam1104I CTCTTC 3 cut(s) 230, 622, 792
EarI CTCTTC 3 cut(s) 230, 622, 792
EciI GGCGGA 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 290
Eco31I GGTCTC 3 cut(s) 301, 624, 647
Eco47I GGWCC 2 cut(s) 530, 1124
Eco57I CTGAAG 1 cut(s) 720
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoRI GAATTC 2 cut(s) 68, 363
EcoRII CCWGG 3 cut(s) 358, 568, 637
EcoT14I CCWWGG 1 cut(s) 290
EcoT22I ATGCAT 2 cut(s) 182, 1135
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 7 cut(s) 63, 122, 180, 451, 759, 881, 1106
FalI AAGNNNNNCTT 2 cut(s) 800, 832
FatI CATG 7 cut(s) 59, 118, 176, 447, 755, 877, 1102
FauNDI CATATG 1 cut(s) 1129
FbaI TGATCA 2 cut(s) 469, 601
Fnu4HI GCNGC 2 cut(s) 316, 696
FokI GGATG 5 cut(s) 453, 464, 595, 763, 845
Fsp4HI GCNGC 2 cut(s) 316, 696
FspBI CTAG 4 cut(s) 140, 144, 312, 332
GluI GCNGC 2 cut(s) 316, 696
GsaI CCCAGC 1 cut(s) 151
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 4 cut(s) 117, 540, 568, 930
HapII CCGG 1 cut(s) 1030
Hin1II CATG 7 cut(s) 63, 122, 180, 451, 759, 881, 1106
HinfI GANTC 3 cut(s) 27, 279, 804
HpaII CCGG 1 cut(s) 1030
HphI GGTGA 2 cut(s) 611, 976
Hpy166II GTNNAC 3 cut(s) 304, 769, 873
Hpy188I TCNGA 4 cut(s) 32, 433, 652, 1120
Hpy188III TCNNGA 7 cut(s) 105, 111, 184, 203, 517, 780, 808
Hpy8I GTNNAC 3 cut(s) 304, 769, 873
HpyAV CCTTC 2 cut(s) 241, 514
HpyCH4III ACNGT 1 cut(s) 168
HpyCH4IV ACGT 3 cut(s) 462, 687, 820
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 668
HpyF3I CTNAG 2 cut(s) 269, 651
HpySE526I ACGT 3 cut(s) 462, 687, 820
Hsp92II CATG 7 cut(s) 63, 122, 180, 451, 759, 881, 1106
Ksp22I TGATCA 2 cut(s) 469, 601
Kzo9I GATC 6 cut(s) 107, 469, 601, 654, 981, 1039
LmnI GCTCC 2 cut(s) 20, 897
Lsp1109I GCAGC 2 cut(s) 302, 682
LweI GCATC 5 cut(s) 189, 240, 739, 1142, 1177
MaeI CTAG 4 cut(s) 140, 144, 312, 332
MaeII ACGT 3 cut(s) 462, 687, 820
MaeIII GTNAC 2 cut(s) 463, 848
MalI GATC 6 cut(s) 109, 471, 603, 656, 983, 1041
MboI GATC 6 cut(s) 107, 469, 601, 654, 981, 1039
MboII GAAGA 8 cut(s) 72, 217, 571, 639, 732, 809, 1013, 1166
MflI RGATCY 2 cut(s) 107, 654
MhlI GDGCHC 1 cut(s) 275
MlsI TGGCCA 1 cut(s) 568
MluCI AATT 4 cut(s) 51, 68, 363, 621
MluNI TGGCCA 1 cut(s) 568
MlyI GAGTC 2 cut(s) 21, 798
MmeI TCCRAC 3 cut(s) 113, 562, 997
MnlI CCTC 8 cut(s) 107, 237, 413, 439, 686, 823, 919, 943
Mox20I TGGCCA 1 cut(s) 568
Mph1103I ATGCAT 2 cut(s) 182, 1135
MscI TGGCCA 1 cut(s) 568
MseI TTAA 1 cut(s) 684
MslI CAYNNNNRTG 3 cut(s) 452, 878, 1082
Msp20I TGGCCA 1 cut(s) 568
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 1 cut(s) 1030
MspR9I CCNGG 3 cut(s) 360, 570, 639
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 3 cut(s) 360, 570, 639
MwoI GCNNNNNNNGC 2 cut(s) 123, 668
NdeI CATATG 1 cut(s) 1129
NdeII GATC 6 cut(s) 107, 469, 601, 654, 981, 1039
NheI GCTAGC 2 cut(s) 139, 143
NlaIII CATG 7 cut(s) 63, 122, 180, 451, 759, 881, 1106
NlaIV GGNNCC 1 cut(s) 893
NmuCI GTSAC 2 cut(s) 463, 848
NsiI ATGCAT 2 cut(s) 182, 1135
NspI RCATGY 2 cut(s) 180, 759
OliI CACNNNNGTG 1 cut(s) 878
PctI GAATGC 1 cut(s) 589
PfeI GAWTC 1 cut(s) 279
PflMI CCANNNNNTGG 1 cut(s) 766
PkrI GCNGC 2 cut(s) 317, 697
PleI GAGTC 2 cut(s) 21, 798
PpsI GAGTC 2 cut(s) 21, 798
Ppu21I YACGTR 1 cut(s) 821
Psp6I CCWGG 3 cut(s) 358, 568, 637
PspFI CCCAGC 1 cut(s) 147
PspGI CCWGG 3 cut(s) 358, 568, 637
PspN4I GGNNCC 1 cut(s) 893
PspPI GGNCC 4 cut(s) 530, 538, 928, 1124
PstI CTGCAG 1 cut(s) 320
PstNI CAGNNNCTG 2 cut(s) 110, 324
PsuI RGATCY 2 cut(s) 107, 654
RsaI GTAC 3 cut(s) 252, 328, 387
RsaNI GTAC 3 cut(s) 251, 327, 386
RseI CAYNNNNRTG 3 cut(s) 452, 878, 1082
SaqAI TTAA 1 cut(s) 684
SatI GCNGC 2 cut(s) 316, 696
Sau3AI GATC 6 cut(s) 107, 469, 601, 654, 981, 1039
Sau96I GGNCC 4 cut(s) 530, 538, 928, 1124
ScaI AGTACT 1 cut(s) 387
SchI GAGTC 2 cut(s) 21, 798
ScrFI CCNGG 3 cut(s) 360, 570, 639
SduI GDGCHC 1 cut(s) 275
SfaNI GCATC 5 cut(s) 189, 240, 739, 1142, 1177
SfcI CTRYAG 2 cut(s) 316, 594
SinI GGWCC 2 cut(s) 530, 1124
SmiMI CAYNNNNRTG 3 cut(s) 452, 878, 1082
SmlI CTYRAG 1 cut(s) 806
SmoI CTYRAG 1 cut(s) 806
Sse9I AATT 4 cut(s) 51, 68, 363, 621
SsiI CCGC 3 cut(s) 438, 634, 660
SspMI CTAG 4 cut(s) 140, 144, 312, 332
StyD4I CCNGG 3 cut(s) 358, 568, 637
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 168
TaiI ACGT 3 cut(s) 465, 690, 823
TaqI TCGA 3 cut(s) 204, 516, 795
TasI AATT 4 cut(s) 51, 68, 363, 621
TatI WGTACW 1 cut(s) 385
TfiI GAWTC 1 cut(s) 279
Tru1I TTAA 1 cut(s) 684
Tru9I TTAA 1 cut(s) 684
TscAI CASTG 4 cut(s) 325, 472, 1030, 1084
TseFI GTSAC 2 cut(s) 463, 848
TseI GCWGC 2 cut(s) 315, 695
Tsp45I GTSAC 2 cut(s) 463, 848
TspDTI ATGAA 1 cut(s) 436
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 4 cut(s) 325, 472, 1030, 1084
Van91I CCANNNNNTGG 1 cut(s) 766
VpaK11BI GGWCC 2 cut(s) 530, 1124
XagI CCTNNNNNAGG 1 cut(s) 335
XapI RAATTY 2 cut(s) 68, 363
XceI RCATGY 2 cut(s) 180, 759
XcmI CCANNNNNNNNNTGG 1 cut(s) 862
XspI CTAG 4 cut(s) 140, 144, 312, 332
ZrmI AGTACT 1 cut(s) 387
Zsp2I ATGCAT 2 cut(s) 182, 1135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.