Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006284

Physical Location & Seq

Genomic Coordinates Forward (+)

1281671 .. 1292253

Gene Structure 10583 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1008800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGGCTAGAGTTTGTTCGCCATACTTTGATCCTGAGTATGAGAACCTGAGCACAAGAATCAATCCCCCAAGGGTCTCCGTAGACAACTCTAGCTGCAGTGACTGTACTCTAGTCAAGGTTGACAGTGTCAACAAACCGGGAATTCTGCTTGAAGTTGTGCAAATCTTGACAGACCTTGACCTCATAATCACCAAAGCCTATATCTCCTCCGATGGTGGATGGTTCATGGATGTATTTCACGTCACCGATCAGCAAGGAAAAAAAGTTACAGACAGTAAGACCATTGACTACATAGAGAAGGCTTTAGGACCAAAGGGCCATATCACAGACCTGCTGAATGCTTGGCCAGGAAAAGGGGTTGGAGTGCACTCTATCGGGGATCATACGGCCATTGAGCTAATTGGCAGAGACCGACCAGGTCTCTTATCTGAGATCTCTGCTGTCCTTGCCAACCTTCACTTTAATGTAATTGCTGCCGACGTATGGACACACAATGGACGAATAGCTTGCGTTGTGTATGTCAATGATGATACTACATGCCAACCTGTGGAGGATCAAGCCAGGTTATCTACTATGGAGGAGCAGCTCAAGAACATCCTGCGTGGGTGTGAGGACGATGAGAAAGTTGGTCGCACTAGCTTCTCTATGGGGTTCACCCATGTTGATAGGCGGCTCCACCAGATGTTGTTTGCTGATAGGGATTATGAGGGTGGTGGACTGGCACATGAAGTTGATCACTTTCCTCCCTGCTTCCAACCAAAGATCGCCATTGAGCGTTGTGAAGAAAAGGGGTACTCGGTGGTTAGTGTGCGGTGCAAGGACCGTGCAAAGCTTATGTTTGACATCGTATGCACACTCACAGATATGCAGTATGTTGTTTTCCATGCCACCATCTCATCTGATGGCCCATACGCATCACAGGAGTATTTTATTCGTCACATGGATGGTTGCACCCTCGATACTGAAGGAGAAAAGGAAAGAGCCATCAAATGTATTGATGCTGCAATTCGAAGAAGAGTAAGCGAGGGTCTAAGGCTGGAGCTGTGTGCAAAGGATAGAGTAGGCTTGCTTTCTGAAGTAACAAGGATCCTGAGAGAGAATGGATTGTCAGTTACAAGAGCAGGCGTCACAACAATTGGGGATCAAGGAATTAATATCTTTTATGTTAGAGATTCTTCAGGGAATTCTGTGGACATAAAGACAATCGAAGCACTTCGTAAAGAAATCGGACACACAATGATGTTCAATGTGAAGAAAGTCCCAACAAGTTCTGCTGAGGTGCCTGAGACTAAAGGATGGGCCAAGACAAGCTTCTTCTTTGGGAACTTGCTAGAGAGGTTCTTGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACT

Protein Analysis

450

Amino Acids

50.05

Weight (kDa)

5.6

Isoelectric Point (pI)

34.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT	PF01842	37 - 86	4.7e-08	ACT domain
ACT	PF01842	130 - 200	5.3e-09	ACT domain
ACT	PF01842	267 - 326	2e-06	ACT domain
ACT	PF01842	346 - 408	3.9e-10	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006284)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76990 AT1G76990 AT1G76990 AT1G76990 AT1G76990
fragaria_vesca	FvH4_2g15200
malus_domestica	MD05G1010200.v1.1 MD05G1010300.v1.1 MD10G1008800.v1.1
prunus_persica	Prupe.8G012600_v2.0.a1 Prupe.8G012600_v2.0.a1 Prupe.8G012600_v2.0.a1 Prupe.8G012600_v2.0.a1 Prupe.8G012600_v2.0.a1
pyrus_communis	pycom05g00530 pycom10g00530
rosa_chinensis	RchiOBHm_Chr6g0277951
rosa_laevigata	RLG00000013254
rosa_multiflora	Rmu_sc0001474.1_g000003
rosa_roxburghii	Rroxscaffold_1G00047070 Rroxscaffold_7G00190650
rosa_rugosa	Rorug06G0113000
rosa_samantha	Rh6CG230000 Rh6DG220700 Rh6DG220800
rosa_wichuraiana	Rw5G024040 Rw6G019510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	339
AccB1I	GGYRCC	1 cut(s)	1279
AccB7I	CCANNNNNTGG	1 cut(s)	547
AccI	GTMKAC	1 cut(s)	81
AciI	CCGC	2 cut(s)	670, 811
AclWI	GGATC	6 cut(s)	23, 387, 561, 1081, 1094, 1149
AcoI	YGGCCR	2 cut(s)	344, 387
AcsI	RAATTY	2 cut(s)	141, 1183
AcuI	CTGAAG	3 cut(s)	984, 1095, 1161
AcyI	GRCGYC	1 cut(s)	1125
AfaI	GTAC	2 cut(s)	106, 794
AfiI	CCNNNNNNNGG	3 cut(s)	353, 483, 547
AgsI	TTSAA	2 cut(s)	152, 1246
AjiI	CACGTC	1 cut(s)	241
AjnI	CCWGG	3 cut(s)	346, 415, 560
AjuI	GAANNNNNNNTTGG	2 cut(s)	1295, 1327
AluBI	AGCT	8 cut(s)	93, 397, 506, 586, 639, 832, 1042, 1311
AluI	AGCT	8 cut(s)	93, 397, 506, 586, 639, 832, 1042, 1311
Alw21I	GWGCWC	2 cut(s)	53, 369
Alw26I	GTCTC	4 cut(s)	79, 402, 425, 1280
Alw44I	GTGCAC	1 cut(s)	365
AlwI	GGATC	6 cut(s)	23, 387, 561, 1081, 1094, 1149
AlwNI	CAGNNNCTG	1 cut(s)	102
AoxI	GGCC	5 cut(s)	316, 344, 387, 904, 1299
ApaLI	GTGCAC	1 cut(s)	365
ApeKI	GCWGC	4 cut(s)	93, 473, 583, 1001
ApoI	RAATTY	2 cut(s)	141, 1183
AseI	ATTAAT	1 cut(s)	1152
Asp700I	GAANNNNTTC	1 cut(s)	1212
AspS9I	GGNCC	5 cut(s)	308, 316, 820, 905, 1299
AsuC2I	CCSGG	1 cut(s)	138
AsuHPI	GGTGA	3 cut(s)	181, 235, 646
AsuII	TTCGAA	1 cut(s)	1009
AvaII	GGWCC	2 cut(s)	308, 820
BaeGI	GKGCMC	1 cut(s)	369
BalI	TGGCCA	1 cut(s)	346
BamHI	GGATCC	1 cut(s)	1086
BanI	GGYRCC	1 cut(s)	1279
Bbv12I	GWGCWC	2 cut(s)	53, 369
BbvCI	CCTCAGC	1 cut(s)	1275
BbvI	GCAGC	4 cut(s)	80, 460, 595, 988
BccI	CCATC	7 cut(s)	206, 213, 896, 899, 938, 992, 1290
BceAI	ACGGC	1 cut(s)	402
BcgI	CGANNNNNNTGC	2 cut(s)	489, 523
BciT130I	CCWGG	3 cut(s)	348, 417, 562
BclI	TGATCA	1 cut(s)	733
BcnI	CCSGG	1 cut(s)	138
BcoDI	GTCTC	4 cut(s)	79, 402, 425, 1280
BfaI	CTAG	5 cut(s)	6, 90, 110, 636, 1331
BfmI	CTRYAG	1 cut(s)	94
BfuAI	ACCTGC	1 cut(s)	339
BglII	AGATCT	1 cut(s)	432
BisI	GCNGC	5 cut(s)	94, 474, 584, 671, 1002
BlsI	GCNGC	5 cut(s)	95, 475, 585, 672, 1003
Bme1390I	CCNGG	4 cut(s)	138, 348, 417, 562
Bme18I	GGWCC	2 cut(s)	308, 820
BmgBI	CACGTC	1 cut(s)	241
BmgT120I	GGNCC	5 cut(s)	308, 316, 820, 905, 1299
BmiI	GGNNCC	3 cut(s)	674, 1088, 1281
BmrFI	CCNGG	4 cut(s)	138, 348, 417, 562
BmsI	GCATC	2 cut(s)	923, 988
BpmI	CTGGAG	1 cut(s)	1058
Bpu10I	CCTNAGC	2 cut(s)	47, 1275
Bpu14I	TTCGAA	1 cut(s)	1009
BpuEI	CTTGAG	1 cut(s)	572
BpuMI	CCSGG	1 cut(s)	138
BsaHI	GRCGYC	1 cut(s)	1125
BsaI	GGTCTC	3 cut(s)	79, 402, 425
BsaJI	CCNNGG	1 cut(s)	68
Bsc4I	CCNNNNNNNGG	3 cut(s)	353, 483, 547
Bse1I	ACTGG	1 cut(s)	723
BseBI	CCWGG	3 cut(s)	348, 417, 562
BseDI	CCNNGG	1 cut(s)	68
BseGI	GGATG	5 cut(s)	224, 235, 594, 949, 1301
BseLI	CCNNNNNNNGG	3 cut(s)	353, 483, 547
BseMII	CTCAG	6 cut(s)	24, 38, 420, 1082, 1266, 1275
BseNI	ACTGG	1 cut(s)	723
BseRI	GAGGAG	2 cut(s)	196, 593
BseSI	GKGCMC	1 cut(s)	369
BseXI	GCAGC	4 cut(s)	80, 460, 595, 988
BshFI	GGCC	5 cut(s)	318, 346, 389, 906, 1301
BshNI	GGYRCC	1 cut(s)	1279
BsiHKAI	GWGCWC	2 cut(s)	53, 369
BsiSI	CCGG	1 cut(s)	137
BslFI	GGGAC	1 cut(s)	1244
BslI	CCNNNNNNNGG	3 cut(s)	353, 483, 547
BsmAI	GTCTC	4 cut(s)	79, 402, 425, 1280
BsmFI	GGGAC	1 cut(s)	1244
BsmI	GAATGC	1 cut(s)	343
BsnI	GGCC	5 cut(s)	318, 346, 389, 906, 1301
Bso31I	GGTCTC	3 cut(s)	79, 402, 425
Bsp119I	TTCGAA	1 cut(s)	1009
Bsp1286I	GDGCHC	2 cut(s)	53, 369
Bsp143I	GATC	9 cut(s)	28, 247, 379, 432, 553, 733, 762, 1086, 1141
BspACI	CCGC	2 cut(s)	670, 811
BspANI	GGCC	5 cut(s)	318, 346, 389, 906, 1301
BspCNI	CTCAG	6 cut(s)	25, 39, 421, 1083, 1267, 1276
BspLI	GGNNCC	3 cut(s)	674, 1088, 1281
BspMAI	CTGCAG	1 cut(s)	98
BspMI	ACCTGC	1 cut(s)	339
BspPI	GGATC	6 cut(s)	23, 387, 561, 1081, 1094, 1149
BspT104I	TTCGAA	1 cut(s)	1009
BspT107I	GGYRCC	1 cut(s)	1279
BspTNI	GGTCTC	3 cut(s)	79, 402, 425
BsrI	ACTGG	1 cut(s)	723
BssECI	CCNNGG	1 cut(s)	68
BssMI	GATC	9 cut(s)	28, 247, 379, 432, 553, 733, 762, 1086, 1141
BssNI	GRCGYC	1 cut(s)	1125
BssT1I	CCWWGG	1 cut(s)	68
Bst2UI	CCWGG	3 cut(s)	348, 417, 562
Bst4CI	ACNGT	4 cut(s)	104, 125, 275, 824
Bst6I	CTCTTC	1 cut(s)	1009
BstACI	GRCGYC	1 cut(s)	1125
BstBI	TTCGAA	1 cut(s)	1009
BstC8I	GCNNGC	3 cut(s)	508, 1067, 1123
BstDEI	CTNAG	7 cut(s)	33, 47, 429, 1031, 1091, 1275, 1284
BstF5I	GGATG	5 cut(s)	224, 235, 594, 949, 1301
BstKTI	GATC	9 cut(s)	31, 250, 382, 435, 556, 736, 765, 1089, 1144
BstMAI	GTCTC	4 cut(s)	79, 402, 425, 1280
BstMBI	GATC	9 cut(s)	28, 247, 379, 432, 553, 733, 762, 1086, 1141
BstMWI	GCNNNNNNNGC	1 cut(s)	446
BstNI	CCWGG	3 cut(s)	348, 417, 562
BstNSI	RCATGY	1 cut(s)	540
BstSCI	CCNGG	4 cut(s)	136, 346, 415, 560
BstSFI	CTRYAG	1 cut(s)	94
BstSLI	GKGCMC	1 cut(s)	369
BstV1I	GCAGC	4 cut(s)	80, 460, 595, 988
BstX2I	RGATCY	2 cut(s)	432, 1086
BstYI	RGATCY	2 cut(s)	432, 1086
BsuRI	GGCC	5 cut(s)	318, 346, 389, 906, 1301
BtrI	CACGTC	1 cut(s)	241
BtsCI	GGATG	5 cut(s)	224, 235, 594, 949, 1301
BtsI	GCAGTG	1 cut(s)	103
BtsIMutI	CAGTG	2 cut(s)	103, 130
BveI	ACCTGC	1 cut(s)	339
Cac8I	GCNNGC	3 cut(s)	508, 1067, 1123
CaiI	CAGNNNCTG	1 cut(s)	102
Cfr13I	GGNCC	5 cut(s)	308, 316, 820, 905, 1299
CseI	GACGC	1 cut(s)	1114
CsiI	ACCWGGT	1 cut(s)	415
Csp6I	GTAC	2 cut(s)	105, 793
CviAII	CATG	6 cut(s)	226, 537, 659, 725, 884, 940
CviQI	GTAC	2 cut(s)	105, 793
DdeI	CTNAG	7 cut(s)	33, 47, 429, 1031, 1091, 1275, 1284
DpnI	GATC	9 cut(s)	30, 249, 381, 434, 555, 735, 764, 1088, 1143
DpnII	GATC	9 cut(s)	28, 247, 379, 432, 553, 733, 762, 1086, 1141
EaeI	YGGCCR	2 cut(s)	344, 387
Eam1104I	CTCTTC	1 cut(s)	1009
EarI	CTCTTC	1 cut(s)	1009
Eco130I	CCWWGG	1 cut(s)	68
Eco31I	GGTCTC	3 cut(s)	79, 402, 425
Eco47I	GGWCC	2 cut(s)	308, 820
Eco57I	CTGAAG	3 cut(s)	984, 1095, 1161
EcoRI	GAATTC	2 cut(s)	141, 1183
EcoRII	CCWGG	3 cut(s)	346, 415, 560
EcoT14I	CCWWGG	1 cut(s)	68
ErhI	CCWWGG	1 cut(s)	68
FaeI	CATG	6 cut(s)	229, 540, 662, 728, 887, 943
FalI	AAGNNNNNCTT	2 cut(s)	1295, 1327
FaqI	GGGAC	1 cut(s)	1244
FatI	CATG	6 cut(s)	225, 536, 658, 724, 883, 939
FbaI	TGATCA	1 cut(s)	733
FblI	GTMKAC	1 cut(s)	81
Fnu4HI	GCNGC	5 cut(s)	94, 474, 584, 671, 1002
FokI	GGATG	5 cut(s)	231, 242, 581, 956, 1308
Fsp4HI	GCNGC	5 cut(s)	94, 474, 584, 671, 1002
FspBI	CTAG	5 cut(s)	6, 90, 110, 636, 1331
GluI	GCNGC	5 cut(s)	94, 474, 584, 671, 1002
GsuI	CTGGAG	1 cut(s)	1058
HaeIII	GGCC	5 cut(s)	318, 346, 389, 906, 1301
HapII	CCGG	1 cut(s)	137
HgaI	GACGC	1 cut(s)	1114
Hin1I	GRCGYC	1 cut(s)	1125
Hin1II	CATG	6 cut(s)	229, 540, 662, 728, 887, 943
HincII	GTYRAC	2 cut(s)	121, 130
HindII	GTYRAC	2 cut(s)	121, 130
HindIII	AAGCTT	2 cut(s)	830, 1309
HinfI	GANTC	2 cut(s)	57, 1172
HpaII	CCGG	1 cut(s)	137
HphI	GGTGA	3 cut(s)	181, 235, 646
Hpy166II	GTNNAC	7 cut(s)	82, 121, 130, 367, 654, 716, 1192
Hpy188I	TCNGA	5 cut(s)	211, 430, 901, 1075, 1229
Hpy188III	TCNNGA	4 cut(s)	32, 166, 589, 1090
Hpy8I	GTNNAC	7 cut(s)	82, 121, 130, 367, 654, 716, 1192
Hpy99I	CGWCG	1 cut(s)	482
HpyAV	CCTTC	3 cut(s)	292, 464, 959
HpyCH4III	ACNGT	4 cut(s)	104, 125, 275, 824
HpyCH4IV	ACGT	2 cut(s)	240, 480
HpyF10VI	GCNNNNNNNGC	1 cut(s)	446
HpyF3I	CTNAG	7 cut(s)	33, 47, 429, 1031, 1091, 1275, 1284
HpySE526I	ACGT	2 cut(s)	240, 480
Hsp92I	GRCGYC	1 cut(s)	1125
Hsp92II	CATG	6 cut(s)	229, 540, 662, 728, 887, 943
Ksp22I	TGATCA	1 cut(s)	733
Kzo9I	GATC	9 cut(s)	28, 247, 379, 432, 553, 733, 762, 1086, 1141
LmnI	GCTCC	3 cut(s)	580, 678, 1039
Lsp1109I	GCAGC	4 cut(s)	80, 460, 595, 988
LweI	GCATC	2 cut(s)	923, 988
MabI	ACCWGGT	1 cut(s)	415
MaeI	CTAG	5 cut(s)	6, 90, 110, 636, 1331
MaeII	ACGT	2 cut(s)	240, 480
MaeIII	GTNAC	7 cut(s)	98, 241, 265, 935, 1078, 1111, 1126
MalI	GATC	9 cut(s)	30, 249, 381, 434, 555, 735, 764, 1088, 1143
MboI	GATC	9 cut(s)	28, 247, 379, 432, 553, 733, 762, 1086, 1141
MboII	GAAGA	6 cut(s)	794, 1023, 1026, 1167, 1264, 1306
MfeI	CAATTG	1 cut(s)	1134
MflI	RGATCY	2 cut(s)	432, 1086
MhlI	GDGCHC	2 cut(s)	53, 369
MlsI	TGGCCA	1 cut(s)	346
MluCI	AATT	7 cut(s)	141, 399, 468, 1005, 1134, 1149, 1183
MluNI	TGGCCA	1 cut(s)	346
MmeI	TCCRAC	2 cut(s)	340, 778
Mox20I	TGGCCA	1 cut(s)	346
MroXI	GAANNNNTTC	1 cut(s)	1212
MscI	TGGCCA	1 cut(s)	346
MseI	TTAA	2 cut(s)	462, 1152
MslI	CAYNNNNRTG	4 cut(s)	462, 863, 942, 1238
Msp20I	TGGCCA	1 cut(s)	346
MspI	CCGG	1 cut(s)	137
MspR9I	CCNGG	4 cut(s)	138, 348, 417, 562
MunI	CAATTG	1 cut(s)	1134
Mva1269I	GAATGC	1 cut(s)	343
MvaI	CCWGG	3 cut(s)	348, 417, 562
MwoI	GCNNNNNNNGC	1 cut(s)	446
NciI	CCSGG	1 cut(s)	138
NdeII	GATC	9 cut(s)	28, 247, 379, 432, 553, 733, 762, 1086, 1141
NlaIII	CATG	6 cut(s)	229, 540, 662, 728, 887, 943
NlaIV	GGNNCC	3 cut(s)	674, 1088, 1281
NmuCI	GTSAC	4 cut(s)	98, 241, 935, 1126
NspI	RCATGY	1 cut(s)	540
NspV	TTCGAA	1 cut(s)	1009
PctI	GAATGC	1 cut(s)	343
PdmI	GAANNNNTTC	1 cut(s)	1212
PfeI	GAWTC	2 cut(s)	57, 1172
PflFI	GACNNNGTC	2 cut(s)	125, 417
PflMI	CCANNNNNTGG	1 cut(s)	547
PkrI	GCNGC	5 cut(s)	95, 475, 585, 672, 1003
PshBI	ATTAAT	1 cut(s)	1152
Psp6I	CCWGG	3 cut(s)	346, 415, 560
PspGI	CCWGG	3 cut(s)	346, 415, 560
PspN4I	GGNNCC	3 cut(s)	674, 1088, 1281
PspPI	GGNCC	5 cut(s)	308, 316, 820, 905, 1299
PstI	CTGCAG	1 cut(s)	98
PstNI	CAGNNNCTG	1 cut(s)	102
PsuI	RGATCY	2 cut(s)	432, 1086
PsyI	GACNNNGTC	2 cut(s)	125, 417
RsaI	GTAC	2 cut(s)	106, 794
RsaNI	GTAC	2 cut(s)	105, 793
RseI	CAYNNNNRTG	4 cut(s)	462, 863, 942, 1238
SaqAI	TTAA	2 cut(s)	462, 1152
SatI	GCNGC	5 cut(s)	94, 474, 584, 671, 1002
Sau3AI	GATC	9 cut(s)	28, 247, 379, 432, 553, 733, 762, 1086, 1141
Sau96I	GGNCC	5 cut(s)	308, 316, 820, 905, 1299
ScrFI	CCNGG	4 cut(s)	138, 348, 417, 562
SduI	GDGCHC	2 cut(s)	53, 369
SexAI	ACCWGGT	1 cut(s)	415
SfaNI	GCATC	2 cut(s)	923, 988
SfcI	CTRYAG	1 cut(s)	94
SfuI	TTCGAA	1 cut(s)	1009
SinI	GGWCC	2 cut(s)	308, 820
SmiMI	CAYNNNNRTG	4 cut(s)	462, 863, 942, 1238
SmlI	CTYRAG	1 cut(s)	587
SmoI	CTYRAG	1 cut(s)	587
Sse9I	AATT	7 cut(s)	141, 399, 468, 1005, 1134, 1149, 1183
SsiI	CCGC	2 cut(s)	670, 811
SspMI	CTAG	5 cut(s)	6, 90, 110, 636, 1331
StyD4I	CCNGG	4 cut(s)	136, 346, 415, 560
StyI	CCWWGG	1 cut(s)	68
TaaI	ACNGT	4 cut(s)	104, 125, 275, 824
TaiI	ACGT	2 cut(s)	243, 483
TaqI	TCGA	3 cut(s)	957, 1009, 1206
TaqII	GACCGA	1 cut(s)	426
TasI	AATT	7 cut(s)	141, 399, 468, 1005, 1134, 1149, 1183
TatI	WGTACW	1 cut(s)	104
TauI	GCSGC	1 cut(s)	673
TfiI	GAWTC	2 cut(s)	57, 1172
Tru1I	TTAA	2 cut(s)	462, 1152
Tru9I	TTAA	2 cut(s)	462, 1152
TscAI	CASTG	2 cut(s)	103, 130
TseFI	GTSAC	4 cut(s)	98, 241, 935, 1126
TseI	GCWGC	4 cut(s)	93, 473, 583, 1001
Tsp45I	GTSAC	4 cut(s)	98, 241, 935, 1126
TspDTI	ATGAA	2 cut(s)	214, 741
TspGWI	ACGGA	1 cut(s)	67
TspRI	CASTG	2 cut(s)	103, 130
Tth111I	GACNNNGTC	2 cut(s)	125, 417
Van91I	CCANNNNNTGG	1 cut(s)	547
VneI	GTGCAC	1 cut(s)	365
VpaK11BI	GGWCC	2 cut(s)	308, 820
VspI	ATTAAT	1 cut(s)	1152
XapI	RAATTY	2 cut(s)	141, 1183
XceI	RCATGY	1 cut(s)	540
XmiI	GTMKAC	1 cut(s)	81
XmnI	GAANNNNTTC	1 cut(s)	1212
XspI	CTAG	5 cut(s)	6, 90, 110, 636, 1331

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.