Rorug06G0113000

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006284
Physical Location & Seq
Forward (+)
15384695 .. 15386003
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0113000.1

Sequence Viewer

Length: 1191 bp
ATGGGCGGTGTTCAAGCAAAGCCAGAGAGCGGACGTGCCGGTTTGGAGAGAAAGACATCCGCTAAGAATCATAGGGTTGTTCCCATCAATGCCGAACCAAGGCCAACTGATGACCTCTGCAAGGATTTTGAAGATGGCAAAGACTTGCAAGACTTCGCCGCCGCACTCGGCTCCAGCGCCAAAGAAGTCTTCTGCAGTACCGGTTTTGATCAGCTTAAGGAATGCATAAAGTGTCTGACGGATTCCGACGAGAAGATGAAGGGGGTAATCTTGTCATGCATAACACAGATACTTGAGGCAGAGGATGTGCAGGCGATGACGGAGAATGAGAAGATCTTAGATGATTTGATCAAAATGTACACCAACAATAGCGGGCAGACGCATCAACTCTGCGGAGTGGAATTACGAAATTTCATCGACTCGGCTCGTGCGGTCCATTCATGCATTACAGATGTGCTTGAGAGGGGAGTGTCGAAGAAGCTCAAAGAGTCTGTGAGGAAGTATCGCCTTGATGCGGAAGCAGTAGCTGCAAAAGTGAAATCTCTGCTCGACCAACATAGAGACATGCTTGACAAGTTGAAAGATCAAATTCAGAAGTGTGATAAGAAGCTGGAGCGTACTCGTACTTTAAAGAAGGTCGTAAATGTGGTGTTCATAGGTATGTTGTCTGGTCTGTTTGTTTGCACCATCATGGCGGCGGCTATGGCTGCTCCGCCAGTTGCGTCAGCCTTGACCTCTCATCCATGTATTGCCGGAGTTATTGCTGGTGGTGCCGTTGGTTATGCTTGTTCGTCAGTATTAAAAGAGGGACGACACTGGCTTCTCACTTTGATTCAAGATTGTGAATCAACTCTGGAGGCACATAAGGGTGTAATTCAGTCGATGGAAGCCGGAACCGGAGTCGCAATTAAAGAGTTGGATGACATCATTTGTCTTGTTGAGAAGGTCATTGCAAAGGGGAATGATCCAGTGTTGTCCAACTCTGCAAATGATAAGGGAAGAGTAGATGTGGAAATTGATGAGAAAGAGATTATTGAAAAAAAGCGGACAGAGTTTCACCAAGCAATTGAGGAATTGGACAAGAAAAGAGAGGACTGCCTCGGGGTTGTAGAGAAGGCAAGCAAAAAGGTTCAAAGGGAATATGACGAATTGAAGAGGAGCCCAGTGTTGAAGACGAAGATCAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0016597 GO:0031406 GO:0036094 GO:0043167 GO:0043168 GO:0043177 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

396

Amino Acids

43.6

Weight (kDa)

6.73

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 171 - 377 9.8e-23 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 770
AccBSI CCGCTC 1 cut(s) 30
AclWI GGATC 1 cut(s) 959
AcsI RAATTY 2 cut(s) 409, 588
AfaI GTAC 4 cut(s) 199, 359, 619, 625
AfiI CCNNNNNNNGG 4 cut(s) 29, 99, 121, 514
AflII CTTAAG 1 cut(s) 215
AgeI ACCGGT 1 cut(s) 200
AgsI TTSAA 8 cut(s) 14, 131, 580, 836, 1037, 1133, 1153, 1171
AjiI CACGTC 1 cut(s) 35
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 4 cut(s) 214, 481, 527, 610
AluI AGCT 4 cut(s) 214, 481, 527, 610
Alw26I GTCTC 1 cut(s) 555
AlwI GGATC 1 cut(s) 959
AlwNI CAGNNNCTG 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 1100
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 2 cut(s) 527, 707
ApoI RAATTY 2 cut(s) 409, 588
AsiGI ACCGGT 1 cut(s) 200
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 433
AsuHPI GGTGA 1 cut(s) 1049
AvaI CYCGRG 1 cut(s) 1100
AvaII GGWCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 770
BanII GRGCYC 1 cut(s) 1163
BauI CACGAG 1 cut(s) 426
BbsI GAAGAC 2 cut(s) 181, 1178
BbvI GCAGC 2 cut(s) 514, 694
BccI CCATC 4 cut(s) 92, 128, 695, 877
BceAI ACGGC 1 cut(s) 758
BclI TGATCA 2 cut(s) 208, 348
BcoDI GTCTC 1 cut(s) 555
BfmI CTRYAG 1 cut(s) 193
BfoI RGCGCY 1 cut(s) 180
BfrI CTTAAG 1 cut(s) 215
BglII AGATCT 1 cut(s) 333
BisI GCNGC 6 cut(s) 159, 162, 528, 696, 699, 708
BlsI GCNGC 6 cut(s) 160, 163, 529, 697, 700, 709
Bme18I GGWCC 1 cut(s) 433
BmeT110I CYCGRG 1 cut(s) 1100
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 433
BmiI GGNNCC 4 cut(s) 172, 772, 895, 1160
BmrI ACTGGG 1 cut(s) 1157
BmsI GCATC 2 cut(s) 391, 502
BmuI ACTGGG 1 cut(s) 1157
BpiI GAAGAC 2 cut(s) 181, 1178
BpmI CTGGAG 3 cut(s) 157, 632, 875
BpuEI CTTGAG 2 cut(s) 314, 479
BsaJI CCNNGG 2 cut(s) 98, 1099
BsaWI WCCGGW 2 cut(s) 200, 896
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 99, 121, 514
Bse118I RCCGGY 2 cut(s) 38, 200
Bse1I ACTGG 4 cut(s) 716, 821, 968, 1163
Bse3DI GCAATG 1 cut(s) 948
BseDI CCNNGG 2 cut(s) 98, 1099
BseGI GGATG 4 cut(s) 56, 310, 739, 925
BseLI CCNNNNNNNGG 4 cut(s) 29, 99, 121, 514
BseMI GCAATG 1 cut(s) 948
BseNI ACTGG 4 cut(s) 716, 821, 968, 1163
BseRI GAGGAG 1 cut(s) 1171
BseXI GCAGC 2 cut(s) 514, 694
BsgI GTGCAG 1 cut(s) 329
BshFI GGCC 1 cut(s) 103
BshNI GGYRCC 1 cut(s) 770
BshTI ACCGGT 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 1100
BsiSI CCGG 5 cut(s) 39, 201, 753, 891, 897
BslFI GGGAC 1 cut(s) 822
BslI CCNNNNNNNGG 4 cut(s) 29, 99, 121, 514
BsmAI GTCTC 1 cut(s) 555
BsmFI GGGAC 1 cut(s) 822
BsmI GAATGC 1 cut(s) 227
BsnI GGCC 1 cut(s) 103
BsoBI CYCGRG 1 cut(s) 1100
Bsp1286I GDGCHC 1 cut(s) 1163
Bsp1407I TGTACA 1 cut(s) 357
Bsp143I GATC 6 cut(s) 208, 333, 348, 583, 964, 1179
BspANI GGCC 1 cut(s) 103
BspLI GGNNCC 4 cut(s) 172, 772, 895, 1160
BspMAI CTGCAG 1 cut(s) 197
BspPI GGATC 1 cut(s) 959
BspT107I GGYRCC 1 cut(s) 770
BspTI CTTAAG 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 30
BsrDI GCAATG 1 cut(s) 948
BsrFI RCCGGY 2 cut(s) 38, 200
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 4 cut(s) 716, 821, 968, 1163
BssAI RCCGGY 2 cut(s) 38, 200
BssECI CCNNGG 2 cut(s) 98, 1099
BssMI GATC 6 cut(s) 208, 333, 348, 583, 964, 1179
BssSI CACGAG 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 98
Bst2BI CACGAG 1 cut(s) 426
Bst6I CTCTTC 2 cut(s) 994, 1148
BstAFI CTTAAG 1 cut(s) 215
BstAPI GCANNNNNTGC 1 cut(s) 527
BstAUI TGTACA 1 cut(s) 357
BstC8I GCNNGC 3 cut(s) 312, 374, 1120
BstDEI CTNAG 2 cut(s) 63, 337
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 4 cut(s) 56, 310, 739, 925
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 6 cut(s) 211, 336, 351, 586, 967, 1182
BstMAI GTCTC 1 cut(s) 555
BstMBI GATC 6 cut(s) 208, 333, 348, 583, 964, 1179
BstMWI GCNNNNNNNGC 4 cut(s) 527, 704, 707, 770
BstNSI RCATGY 1 cut(s) 568
BstSFI CTRYAG 1 cut(s) 193
BstV1I GCAGC 2 cut(s) 514, 694
BstV2I GAAGAC 2 cut(s) 181, 1178
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BsuRI GGCC 1 cut(s) 103
BtgZI GCGATG 1 cut(s) 329
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 4 cut(s) 56, 310, 739, 925
BtsIMutI CAGTG 3 cut(s) 814, 975, 1170
Cac8I GCNNGC 3 cut(s) 312, 374, 1120
CaiI CAGNNNCTG 1 cut(s) 527
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 2 cut(s) 38, 200
Cfr13I GGNCC 1 cut(s) 433
CseI GACGC 2 cut(s) 388, 711
Csp6I GTAC 4 cut(s) 198, 358, 618, 624
CspAI ACCGGT 1 cut(s) 200
CviAII CATG 5 cut(s) 276, 441, 565, 691, 744
CviQI GTAC 4 cut(s) 198, 358, 618, 624
DdeI CTNAG 2 cut(s) 63, 337
DpnI GATC 6 cut(s) 210, 335, 350, 585, 966, 1181
DpnII GATC 6 cut(s) 208, 333, 348, 583, 964, 1179
DraI TTTAAA 1 cut(s) 630
Eam1104I CTCTTC 2 cut(s) 994, 1148
EarI CTCTTC 2 cut(s) 994, 1148
EciI GGCGGA 1 cut(s) 702
Eco130I CCWWGG 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 1163
Eco47I GGWCC 1 cut(s) 433
Eco88I CYCGRG 1 cut(s) 1100
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoT14I CCWWGG 1 cut(s) 98
EcoT22I ATGCAT 3 cut(s) 227, 281, 446
EcoT38I GRGCYC 1 cut(s) 1163
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 5 cut(s) 279, 444, 568, 694, 747
FaqI GGGAC 1 cut(s) 822
FatI CATG 5 cut(s) 275, 440, 564, 690, 743
FauI CCCGC 1 cut(s) 365
FbaI TGATCA 2 cut(s) 208, 348
Fnu4HI GCNGC 6 cut(s) 159, 162, 528, 696, 699, 708
FokI GGATG 4 cut(s) 43, 317, 726, 932
FriOI GRGCYC 1 cut(s) 1163
Fsp4HI GCNGC 6 cut(s) 159, 162, 528, 696, 699, 708
GlaI GCGC 1 cut(s) 178
GluI GCNGC 6 cut(s) 159, 162, 528, 696, 699, 708
GsuI CTGGAG 3 cut(s) 157, 632, 875
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 1 cut(s) 103
HapII CCGG 5 cut(s) 39, 201, 753, 891, 897
HgaI GACGC 2 cut(s) 388, 711
HhaI GCGC 1 cut(s) 179
Hin1II CATG 5 cut(s) 279, 444, 568, 694, 747
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HinfI GANTC 7 cut(s) 67, 242, 419, 488, 832, 845, 900
HpaII CCGG 5 cut(s) 39, 201, 753, 891, 897
HphI GGTGA 1 cut(s) 1049
Hpy166II GTNNAC 1 cut(s) 360
Hpy188I TCNGA 3 cut(s) 237, 247, 594
Hpy188III TCNNGA 3 cut(s) 836, 854, 1183
Hpy8I GTNNAC 1 cut(s) 360
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 4 cut(s) 253, 628, 937, 1108
HpyCH4IV ACGT 1 cut(s) 34
HpyF10VI GCNNNNNNNGC 4 cut(s) 527, 704, 707, 770
HpyF3I CTNAG 2 cut(s) 63, 337
HpySE526I ACGT 1 cut(s) 34
Hsp92II CATG 5 cut(s) 279, 444, 568, 694, 747
HspAI GCGC 1 cut(s) 177
Ksp22I TGATCA 2 cut(s) 208, 348
Kzo9I GATC 6 cut(s) 208, 333, 348, 583, 964, 1179
LmnI GCTCC 4 cut(s) 176, 613, 715, 1158
Lsp1109I GCAGC 2 cut(s) 514, 694
LweI GCATC 2 cut(s) 391, 502
MaeII ACGT 1 cut(s) 34
MalI GATC 6 cut(s) 210, 335, 350, 585, 966, 1181
MbiI CCGCTC 1 cut(s) 30
MboI GATC 6 cut(s) 208, 333, 348, 583, 964, 1179
MboII GAAGA 9 cut(s) 143, 181, 265, 343, 487, 1011, 1165, 1183, 1189
MfeI CAATTG 1 cut(s) 1065
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 1163
MluCI AATT 9 cut(s) 401, 409, 588, 873, 906, 1014, 1065, 1073, 1148
MlyI GAGTC 3 cut(s) 413, 497, 909
MmeI TCCRAC 3 cut(s) 270, 897, 1002
Mph1103I ATGCAT 3 cut(s) 227, 281, 446
MseI TTAA 4 cut(s) 216, 629, 800, 909
MslI CAYNNNNRTG 3 cut(s) 659, 689, 867
MspCI CTTAAG 1 cut(s) 215
MspI CCGG 5 cut(s) 39, 201, 753, 891, 897
MunI CAATTG 1 cut(s) 1065
Mva1269I GAATGC 1 cut(s) 227
MwoI GCNNNNNNNGC 4 cut(s) 527, 704, 707, 770
NdeII GATC 6 cut(s) 208, 333, 348, 583, 964, 1179
NlaIII CATG 5 cut(s) 279, 444, 568, 694, 747
NlaIV GGNNCC 4 cut(s) 172, 772, 895, 1160
NmeAIII GCCGAG 2 cut(s) 147, 401
NsiI ATGCAT 3 cut(s) 227, 281, 446
NspI RCATGY 1 cut(s) 568
PctI GAATGC 1 cut(s) 227
PfeI GAWTC 4 cut(s) 67, 242, 832, 845
PinAI ACCGGT 1 cut(s) 200
PkrI GCNGC 6 cut(s) 160, 163, 529, 697, 700, 709
PleI GAGTC 3 cut(s) 413, 496, 908
PpsI GAGTC 3 cut(s) 413, 496, 908
PspN4I GGNNCC 4 cut(s) 172, 772, 895, 1160
PspPI GGNCC 1 cut(s) 433
PstI CTGCAG 1 cut(s) 197
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 1 cut(s) 333
RsaI GTAC 4 cut(s) 199, 359, 619, 625
RsaNI GTAC 4 cut(s) 198, 358, 618, 624
RseI CAYNNNNRTG 3 cut(s) 659, 689, 867
SaqAI TTAA 4 cut(s) 216, 629, 800, 909
SatI GCNGC 6 cut(s) 159, 162, 528, 696, 699, 708
Sau3AI GATC 6 cut(s) 208, 333, 348, 583, 964, 1179
Sau96I GGNCC 1 cut(s) 433
SchI GAGTC 3 cut(s) 413, 497, 909
SduI GDGCHC 1 cut(s) 1163
SfaNI GCATC 2 cut(s) 391, 502
SfcI CTRYAG 1 cut(s) 193
SinI GGWCC 1 cut(s) 433
SmiMI CAYNNNNRTG 3 cut(s) 659, 689, 867
SmlI CTYRAG 3 cut(s) 215, 293, 458
SmoI CTYRAG 3 cut(s) 215, 293, 458
Sse9I AATT 9 cut(s) 401, 409, 588, 873, 906, 1014, 1065, 1073, 1148
StyI CCWWGG 1 cut(s) 98
TaiI ACGT 1 cut(s) 37
TaqI TCGA 4 cut(s) 417, 473, 549, 881
TasI AATT 9 cut(s) 401, 409, 588, 873, 906, 1014, 1065, 1073, 1148
TatI WGTACW 1 cut(s) 357
TauI GCSGC 4 cut(s) 161, 164, 698, 701
TfiI GAWTC 4 cut(s) 67, 242, 832, 845
Tru1I TTAA 4 cut(s) 216, 629, 800, 909
Tru9I TTAA 4 cut(s) 216, 629, 800, 909
TscAI CASTG 3 cut(s) 821, 975, 1170
TseI GCWGC 2 cut(s) 527, 707
TspDTI ATGAA 4 cut(s) 272, 403, 429, 643
TspGWI ACGGA 2 cut(s) 254, 335
TspRI CASTG 3 cut(s) 821, 975, 1170
Vha464I CTTAAG 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 433
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 2 cut(s) 409, 588
XceI RCATGY 1 cut(s) 568
Zsp2I ATGCAT 3 cut(s) 227, 281, 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.