AT1G77000

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005717
Physical Location & Seq
Forward (+)
28940147 .. 28942639
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G77000.2

Sequence Viewer

Length: 1083 bp
ATGGTGAGTGAAGGAGCAACAAGAAAAGAACTTAACCTCTGTTTCGAGAATATGAAGATGGAAGGAGTTTTGATCTCTGAGTGGAAAGATATCCCTGTGGAGCTTCTCATGAAGATTTTAAACCTTGTTGATGATCGGACTGTGATCATTGCTTCTTGTATTTGTAGTGGCTGGAGAGATGCTGTTTCCCTTGGCCTCACTCGCCTCTCCCTCTCTTGGTGCAAGAAGAATATGAACAGTTTGGTTCTATCTCTTGCTCCCAAATTCGTAAAGCTTCAGACTTTAGTACTGCGACAGGACAAACCGCAGCTTGAGGACAACGCGGTGGAAGCCATAGCAAATCACTGTCATGAGCTACAAGATTTGGACTTAAGCAAAAGCTCGAAAATCACTGACCATTCCCTATATTCACTTGCTCGTGGTTGTACTAACCTGACTAAACTCAACCTTAGCGGCTGCACTTCGTTCAGCGACACTGCTCTTGCGCATTTGACAAGATTTTGCAGGAAGCTCAAAATTCTGAATCTTTGTGGTTGTGTTGAAGCTGTATCTGACAATACATTGCAGGCTATTGGAGAAAACTGCAATCAGTTGCAGTCACTAAACTTGGGATGGTGTGAGAATATAAGTGATGATGGAGTTATGAGTTTAGCTTATGGTTGTCCTGATTTAAGAACTCTTGATCTTTGTAGCTGTGTTCTAATCACAGATGAGAGTGTTGTGGCTTTGGCGAATCGGTGCATTCACTTGAGGTCATTGGGGTTATACTACTGCAGAAACATTACAGACAGAGCAATGTACTCTTTAGCTCAGAGCGGAGTCAAGAACAAACACGAGATGTGGCGAGCGGTAAAGAAAGGAAAATTCGATGAAGAAGGACTAAGAAGCCTTAACATTAGTCAATGCACTTACCTAACACCTTCAGCTGTTCAAGCTGTCTGTGATACATTCCCTGCTCTCCACACTTGTTCAGGCAGACATTCACTTGTCATGAGCGGTTGTTTGAATCTACAATCTGTTCATTGTGCTTGTATCCTTCAAGCTCACCGCACTCACACCGTTTACCCTCACCCGGCGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010286 GO:0010311 GO:0010498 GO:0016567 GO:0016740 GO:0019005 GO:0019538 GO:0019787 GO:0019941 GO:0022622 GO:0030163 GO:0031146 GO:0031461 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0036211 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048518 GO:0048519 GO:0048522 GO:0048527 GO:0048528 GO:0048580 GO:0048581 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051302 GO:0051603 GO:0051716 GO:0051781 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901332 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905392 GO:1905393 GO:1990234 GO:2000023 GO:2000026 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04068 ko04110 ko04120 ko04150 ko05168 ko05169 ko05200 ko05203 ko05222 map04068 map04110 map04120 map04150 map05168 map05169 map05200 map05203 map05222
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

360

Amino Acids

39.91

Weight (kDa)

7.45

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 104 - 282 3.3e-19 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 486
AccBSI CCGCTC 3 cut(s) 816, 848, 996
AccII CGCG 1 cut(s) 323
AciI CCGC 7 cut(s) 305, 323, 453, 816, 848, 996, 1048
AcsI RAATTY 3 cut(s) 263, 516, 863
AcuI CTGAAG 2 cut(s) 260, 906
AfaI GTAC 3 cut(s) 288, 427, 800
AfiI CCNNNNNNNGG 2 cut(s) 216, 1072
AflII CTTAAG 1 cut(s) 370
AgsI TTSAA 4 cut(s) 542, 932, 1006, 1040
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 2 cut(s) 307, 456
ApoI RAATTY 3 cut(s) 263, 516, 863
AspLEI GCGC 2 cut(s) 487, 1078
AsuC2I CCSGG 1 cut(s) 1073
AsuHPI GGTGA 3 cut(s) 16, 1037, 1061
BauI CACGAG 2 cut(s) 417, 833
BbvI GCAGC 2 cut(s) 319, 443
BccI CCATC 3 cut(s) 52, 606, 629
BciVI GTATCC 1 cut(s) 1043
BclI TGATCA 1 cut(s) 144
BcnI CCSGG 1 cut(s) 1073
BfmI CTRYAG 1 cut(s) 772
BfrI CTTAAG 1 cut(s) 370
BfuI GTATCC 1 cut(s) 1043
BisI GCNGC 3 cut(s) 308, 454, 457
BlsI GCNGC 3 cut(s) 309, 455, 458
BmcAI AGTACT 1 cut(s) 288
Bme1390I CCNGG 1 cut(s) 1073
BmrFI CCNGG 1 cut(s) 1073
BmsI GCATC 1 cut(s) 169
BpmI CTGGAG 1 cut(s) 193
Bpu10I CCTNAGC 1 cut(s) 449
BpuEI CTTGAG 2 cut(s) 332, 769
BpuMI CCSGG 1 cut(s) 1073
BsaJI CCNNGG 1 cut(s) 190
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 1072
Bse3DI GCAATG 3 cut(s) 147, 560, 801
BseDI CCNNGG 1 cut(s) 190
BseGI GGATG 1 cut(s) 617
BseLI CCNNNNNNNGG 2 cut(s) 216, 1072
BseMI GCAATG 3 cut(s) 147, 560, 801
BseMII CTCAG 2 cut(s) 69, 824
BseXI GCAGC 2 cut(s) 319, 443
BsgI GTGCAG 1 cut(s) 442
Bsh1236I CGCG 1 cut(s) 323
BshFI GGCC 1 cut(s) 195
BsiSI CCGG 1 cut(s) 1073
BslI CCNNNNNNNGG 2 cut(s) 216, 1072
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 1 cut(s) 195
Bsp143I GATC 4 cut(s) 72, 133, 144, 682
BspACI CCGC 7 cut(s) 305, 323, 453, 816, 848, 996, 1048
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 2 cut(s) 70, 823
BspFNI CGCG 1 cut(s) 323
BspHI TCATGA 3 cut(s) 108, 349, 990
BspMAI CTGCAG 1 cut(s) 776
BspTI CTTAAG 1 cut(s) 370
BsrBI CCGCTC 3 cut(s) 816, 848, 996
BsrDI GCAATG 3 cut(s) 147, 560, 801
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 4 cut(s) 72, 133, 144, 682
BssSI CACGAG 2 cut(s) 417, 833
BssT1I CCWWGG 1 cut(s) 190
Bst2BI CACGAG 2 cut(s) 417, 833
Bst4CI ACNGT 4 cut(s) 142, 239, 347, 1060
BstAFI CTTAAG 1 cut(s) 370
BstC8I GCNNGC 2 cut(s) 567, 846
BstDEI CTNAG 4 cut(s) 78, 449, 810, 881
BstF5I GGATG 1 cut(s) 617
BstFNI CGCG 1 cut(s) 323
BstHHI GCGC 2 cut(s) 487, 1078
BstKTI GATC 4 cut(s) 75, 136, 147, 685
BstMBI GATC 4 cut(s) 72, 133, 144, 682
BstMWI GCNNNNNNNGC 3 cut(s) 201, 329, 932
BstSCI CCNGG 1 cut(s) 1071
BstSFI CTRYAG 1 cut(s) 772
BstUI CGCG 1 cut(s) 323
BstV1I GCAGC 2 cut(s) 319, 443
BsuI GTATCC 1 cut(s) 1043
BsuRI GGCC 1 cut(s) 195
BtsCI GGATG 1 cut(s) 617
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 3 cut(s) 343, 390, 474
Cac8I GCNNGC 2 cut(s) 567, 846
CciI TCATGA 3 cut(s) 108, 349, 990
CfoI GCGC 2 cut(s) 487, 1078
Csp6I GTAC 3 cut(s) 287, 426, 799
CviAII CATG 3 cut(s) 109, 350, 991
CviQI GTAC 3 cut(s) 287, 426, 799
DdeI CTNAG 4 cut(s) 78, 449, 810, 881
DpnI GATC 4 cut(s) 74, 135, 146, 684
DpnII GATC 4 cut(s) 72, 133, 144, 682
DraI TTTAAA 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 190
Eco32I GATATC 1 cut(s) 91
Eco57I CTGAAG 2 cut(s) 260, 906
EcoRV GATATC 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 190
FaeI CATG 3 cut(s) 112, 353, 994
FatI CATG 3 cut(s) 108, 349, 990
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 3 cut(s) 308, 454, 457
FokI GGATG 1 cut(s) 624
Fsp4HI GCNGC 3 cut(s) 308, 454, 457
FspI TGCGCA 1 cut(s) 486
GlaI GCGC 2 cut(s) 486, 1077
GluI GCNGC 3 cut(s) 308, 454, 457
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 1 cut(s) 195
HapII CCGG 1 cut(s) 1073
HhaI GCGC 2 cut(s) 487, 1078
Hin1II CATG 3 cut(s) 112, 353, 994
Hin6I GCGC 2 cut(s) 485, 1076
HinP1I GCGC 2 cut(s) 485, 1076
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 4 cut(s) 523, 733, 819, 1006
HpaII CCGG 1 cut(s) 1073
HphI GGTGA 3 cut(s) 16, 1037, 1061
Hpy166II GTNNAC 1 cut(s) 1063
Hpy188I TCNGA 6 cut(s) 79, 138, 279, 522, 553, 813
Hpy188III TCNNGA 7 cut(s) 46, 109, 350, 665, 680, 823, 991
Hpy8I GTNNAC 1 cut(s) 1063
HpyAV CCTTC 5 cut(s) 5, 56, 869, 930, 1046
HpyCH4III ACNGT 4 cut(s) 142, 239, 347, 1060
HpyCH4V TGCA 9 cut(s) 222, 459, 504, 565, 585, 595, 741, 774, 906
HpyF10VI GCNNNNNNNGC 3 cut(s) 201, 329, 932
HpyF3I CTNAG 4 cut(s) 78, 449, 810, 881
Hsp92II CATG 3 cut(s) 112, 353, 994
HspAI GCGC 2 cut(s) 485, 1076
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 4 cut(s) 72, 133, 144, 682
LmnI GCTCC 3 cut(s) 14, 100, 262
LpnPI CCDG 9 cut(s) 108, 157, 281, 446, 490, 551, 678, 957, 966
Lsp1109I GCAGC 2 cut(s) 319, 443
LweI GCATC 1 cut(s) 169
MaeIII GTNAC 1 cut(s) 597
MalI GATC 4 cut(s) 74, 135, 146, 684
MbiI CCGCTC 3 cut(s) 816, 848, 996
MboI GATC 4 cut(s) 72, 133, 144, 682
MboII GAAGA 4 cut(s) 67, 124, 238, 884
MluCI AATT 3 cut(s) 263, 516, 863
MlyI GAGTC 1 cut(s) 828
MnlI CCTC 7 cut(s) 47, 206, 215, 221, 307, 744, 1077
MseI TTAA 5 cut(s) 33, 119, 371, 671, 891
MslI CAYNNNNRTG 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 926
MspCI CTTAAG 1 cut(s) 370
MspI CCGG 1 cut(s) 1073
MspR9I CCNGG 1 cut(s) 1073
Mva1269I GAATGC 1 cut(s) 741
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 3 cut(s) 201, 329, 932
NciI CCSGG 1 cut(s) 1073
NdeII GATC 4 cut(s) 72, 133, 144, 682
NlaIII CATG 3 cut(s) 112, 353, 994
NmuCI GTSAC 1 cut(s) 597
NsbI TGCGCA 1 cut(s) 486
PagI TCATGA 3 cut(s) 108, 349, 990
PctI GAATGC 1 cut(s) 741
PfeI GAWTC 3 cut(s) 523, 733, 1006
PkrI GCNGC 3 cut(s) 309, 455, 458
PleI GAGTC 1 cut(s) 827
PpsI GAGTC 1 cut(s) 827
PstI CTGCAG 1 cut(s) 776
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 3 cut(s) 288, 427, 800
RsaNI GTAC 3 cut(s) 287, 426, 799
RseI CAYNNNNRTG 1 cut(s) 348
SaqAI TTAA 5 cut(s) 33, 119, 371, 671, 891
SatI GCNGC 3 cut(s) 308, 454, 457
Sau3AI GATC 4 cut(s) 72, 133, 144, 682
ScaI AGTACT 1 cut(s) 288
SchI GAGTC 1 cut(s) 828
ScrFI CCNGG 1 cut(s) 1073
SfaNI GCATC 1 cut(s) 169
SfcI CTRYAG 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 3 cut(s) 311, 370, 748
SmoI CTYRAG 3 cut(s) 311, 370, 748
Sse9I AATT 3 cut(s) 263, 516, 863
SsiI CCGC 7 cut(s) 305, 323, 453, 816, 848, 996, 1048
StyD4I CCNGG 1 cut(s) 1071
StyI CCWWGG 1 cut(s) 190
TaaI ACNGT 4 cut(s) 142, 239, 347, 1060
TaqI TCGA 3 cut(s) 45, 383, 867
TasI AATT 3 cut(s) 263, 516, 863
TatI WGTACW 3 cut(s) 286, 425, 798
TauI GCSGC 1 cut(s) 456
TfiI GAWTC 3 cut(s) 523, 733, 1006
Tru1I TTAA 5 cut(s) 33, 119, 371, 671, 891
Tru9I TTAA 5 cut(s) 33, 119, 371, 671, 891
TscAI CASTG 3 cut(s) 350, 397, 481
TseFI GTSAC 1 cut(s) 597
TseI GCWGC 2 cut(s) 307, 456
Tsp45I GTSAC 1 cut(s) 597
TspDTI ATGAA 5 cut(s) 68, 125, 248, 885, 1010
TspRI CASTG 3 cut(s) 350, 397, 481
Vha464I CTTAAG 1 cut(s) 370
XapI RAATTY 3 cut(s) 263, 516, 863
ZrmI AGTACT 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.