pycom10g00540

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005717
Physical Location & Seq
Forward (+)
589460 .. 591977
2518 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00540.1

Sequence Viewer

Length: 1092 bp
ATGGTGGGAGAGGGAAACATCAAGGCTGAGGACTTGAACTTGTGCTTTGAGAAGCTGAAGATGGAAGGAAGGGTGATGATCACCGATTGGAAGGATATTCCGATGGAGCTGCTTCTGCAAATCCTGGCGCTTGCCGATGATCAGACGGTGATCACGGCGTCTGGAGTCTGCCGTGGCTGGAGAGATGCTGCTTGCTTGGGCCTTACTCGTCTCTCCCTCTCATGGTGCACCAAGAACATGAACAGCTTGGTCCTATCTCTGGCTCCTAAGTTTACGAGATTGCAGACTCTAATACTGCGGCAAGATCATCAACCCCAACTTCAGGATAATGCTGTTGAGAGCATTGCAAACTTCTGTCATGACCTGCAGGTCCTGGACCTCAGCAAAAGCTTCAAGCTCAGTGATCGATCCCTATATGCCTTGGCTCACGGGTGCCCAAATCTTACAAAACTAAACATTAGTGGGTGCTCGGCATTCAGTGACATTGCTCTTGAATATCTTGTTGGATTTTGCCAAAAATTGGAAGTCTTGAATCTTTGTGGTTGCGTCAGGAGTGCATCAGACAGGGCATTGCAGGCTATTGGGCGTTACTGTAGTCAGTTGCAGTCTCTGAATCTTGGTTGGTGTGAGGATGTAAGTGATGTTGGAGTTATGAGTTTGGCACATGGATGCCCTGATCTCAGAACTGTTGATTTGTGCGGCTGTGTCTTAATATCAGATGATAGTGTGATTGCTTTGGCAAACAGTTGTCCTCATTTGCAATCGCTTGACCTTTACTACTGTAAGAACATTACAGACAAAGCAATGTATTCCTTGGCCCAGAGTCTAGTGAAGAACAAGCCTGCAGCAATGTGGGAATCCCAGAAAGCCAGGAACGACGAGGAAGGGCTTAGGACCCTCAACATCAGCCAATGCACAGCACTCACCCCTTCTGCTGTTCAGGCGGTGTGCGACTCATTCCCTGCACTTCACACCTGCTCTGGAAGGCATTCCCTCATCATTAGCGGCTGTTTGAACTTGACGTCCGTGCATTGCGCCTGTGCTGTCCAAGCTCACCGCACTGCCAACGCCTTTCCTCATTCAGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010286 GO:0010311 GO:0010498 GO:0016567 GO:0016740 GO:0019005 GO:0019538 GO:0019787 GO:0019941 GO:0022622 GO:0030163 GO:0031146 GO:0031461 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0036211 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048518 GO:0048519 GO:0048522 GO:0048527 GO:0048528 GO:0048580 GO:0048581 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051302 GO:0051603 GO:0051716 GO:0051781 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901332 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905392 GO:1905393 GO:1990234 GO:2000023 GO:2000026 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04068 ko04110 ko04120 ko04150 ko05168 ko05169 ko05200 ko05203 ko05222 map04068 map04110 map04120 map04150 map05168 map05169 map05200 map05203 map05222
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

364

Amino Acids

39.63

Weight (kDa)

6.11

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 30 - 65 1.5e-06 F-box-like
DUF7885 PF25372 68 - 275 4.2e-18 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 983
AasI GACNNNNNNGTC 1 cut(s) 368
AatII GACGTC 1 cut(s) 1025
Acc36I ACCTGC 3 cut(s) 358, 372, 983
AccB1I GGYRCC 1 cut(s) 432
AccB7I CCANNNNNTGG 1 cut(s) 520
AciI CCGC 5 cut(s) 298, 699, 944, 1005, 1057
AclWI GGATC 1 cut(s) 402
AcuI CTGAAG 2 cut(s) 77, 305
AcyI GRCGYC 2 cut(s) 158, 1022
AfiI CCNNNNNNNGG 4 cut(s) 222, 259, 322, 520
AgsI TTSAA 5 cut(s) 37, 394, 494, 532, 1015
AjnI CCWGG 3 cut(s) 123, 372, 869
AjuI GAANNNNNNNTTGG 2 cut(s) 486, 518
AloI GAACNNNNNNTCC 2 cut(s) 1007, 1039
AluBI AGCT 7 cut(s) 55, 109, 246, 390, 397, 1052, 1085
AluI AGCT 7 cut(s) 55, 109, 246, 390, 397, 1052, 1085
Alw21I GWGCWC 2 cut(s) 230, 470
Alw26I GTCTC 2 cut(s) 215, 612
Alw44I GTGCAC 1 cut(s) 226
AlwI GGATC 1 cut(s) 402
AlwNI CAGNNNCTG 2 cut(s) 373, 610
AoxI GGCC 2 cut(s) 199, 816
ApaLI GTGCAC 1 cut(s) 226
ApeKI GCWGC 3 cut(s) 109, 188, 845
Asp700I GAANNNNTTC 1 cut(s) 988
AspLEI GCGC 2 cut(s) 130, 1037
AspS9I GGNCC 6 cut(s) 199, 250, 370, 376, 817, 894
AsuHPI GGTGA 5 cut(s) 73, 85, 160, 916, 1046
AvaII GGWCC 4 cut(s) 250, 370, 376, 894
BaeGI GKGCMC 2 cut(s) 230, 437
BanI GGYRCC 1 cut(s) 432
Bbv12I GWGCWC 2 cut(s) 230, 470
BbvCI CCTCAGC 2 cut(s) 27, 380
BbvI GCAGC 3 cut(s) 96, 175, 857
BccI CCATC 2 cut(s) 55, 97
BceAI ACGGC 2 cut(s) 156, 171
BcgI CGANNNNNNTGC 2 cut(s) 91, 125
BciT130I CCWGG 3 cut(s) 125, 374, 871
BclI TGATCA 3 cut(s) 78, 139, 150
BcoDI GTCTC 2 cut(s) 215, 612
BfaI CTAG 1 cut(s) 827
BfmI CTRYAG 3 cut(s) 365, 592, 843
BfoI RGCGCY 1 cut(s) 131
BfuAI ACCTGC 3 cut(s) 358, 372, 983
BisI GCNGC 6 cut(s) 110, 189, 299, 700, 846, 1006
BlsI GCNGC 6 cut(s) 111, 190, 300, 701, 847, 1007
Bme1390I CCNGG 3 cut(s) 125, 374, 871
Bme18I GGWCC 4 cut(s) 250, 370, 376, 894
BmgT120I GGNCC 6 cut(s) 199, 250, 370, 376, 817, 894
BmiI GGNNCC 3 cut(s) 264, 434, 896
BmrFI CCNGG 3 cut(s) 125, 374, 871
BmsI GCATC 3 cut(s) 175, 566, 659
BpmI CTGGAG 2 cut(s) 183, 199
Bpu10I CCTNAGC 3 cut(s) 27, 380, 890
Bsa29I ATCGAT 1 cut(s) 406
BsaHI GRCGYC 2 cut(s) 158, 1022
BsaJI CCNNGG 3 cut(s) 172, 420, 813
Bsc4I CCNNNNNNNGG 4 cut(s) 222, 259, 322, 520
Bse3DI GCAATG 6 cut(s) 342, 483, 569, 810, 855, 1030
BseBI CCWGG 3 cut(s) 125, 374, 871
BseCI ATCGAT 1 cut(s) 406
BseDI CCNNGG 3 cut(s) 172, 420, 813
BseGI GGATG 2 cut(s) 637, 674
BseLI CCNNNNNNNGG 4 cut(s) 222, 259, 322, 520
BseMI GCAATG 6 cut(s) 342, 483, 569, 810, 855, 1030
BseMII CTCAG 4 cut(s) 18, 394, 412, 694
BseSI GKGCMC 2 cut(s) 230, 437
BseXI GCAGC 3 cut(s) 96, 175, 857
BsgI GTGCAG 1 cut(s) 948
BshFI GGCC 2 cut(s) 201, 818
BshNI GGYRCC 1 cut(s) 432
BshVI ATCGAT 1 cut(s) 406
BsiHKAI GWGCWC 2 cut(s) 230, 470
BslI CCNNNNNNNGG 4 cut(s) 222, 259, 322, 520
BsmAI GTCTC 2 cut(s) 215, 612
BsmBI CGTCTC 1 cut(s) 215
BsmI GAATGC 2 cut(s) 473, 988
BsnI GGCC 2 cut(s) 201, 818
Bsp1286I GDGCHC 3 cut(s) 230, 437, 470
Bsp143I GATC 7 cut(s) 78, 139, 150, 304, 403, 407, 676
BspACI CCGC 5 cut(s) 298, 699, 944, 1005, 1057
BspANI GGCC 2 cut(s) 201, 818
BspCNI CTCAG 4 cut(s) 19, 393, 411, 693
BspDI ATCGAT 1 cut(s) 406
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 3 cut(s) 264, 434, 896
BspMAI CTGCAG 2 cut(s) 369, 847
BspMI ACCTGC 3 cut(s) 358, 372, 983
BspPI GGATC 1 cut(s) 402
BspT107I GGYRCC 1 cut(s) 432
BsrDI GCAATG 6 cut(s) 342, 483, 569, 810, 855, 1030
BssECI CCNNGG 3 cut(s) 172, 420, 813
BssMI GATC 7 cut(s) 78, 139, 150, 304, 403, 407, 676
BssNI GRCGYC 2 cut(s) 158, 1022
BssT1I CCWWGG 2 cut(s) 420, 813
Bst2UI CCWGG 3 cut(s) 125, 374, 871
Bst4CI ACNGT 5 cut(s) 148, 593, 688, 746, 782
BstACI GRCGYC 2 cut(s) 158, 1022
BstC8I GCNNGC 4 cut(s) 132, 193, 576, 843
BstDEI CTNAG 6 cut(s) 27, 267, 380, 398, 680, 890
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 2 cut(s) 637, 674
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 2 cut(s) 130, 1037
BstKTI GATC 7 cut(s) 81, 142, 153, 307, 406, 410, 679
BstMAI GTCTC 2 cut(s) 215, 612
BstMBI GATC 7 cut(s) 78, 139, 150, 304, 403, 407, 676
BstMWI GCNNNNNNNGC 4 cut(s) 115, 575, 941, 1049
BstNI CCWGG 3 cut(s) 125, 374, 871
BstSCI CCNGG 3 cut(s) 123, 372, 869
BstSFI CTRYAG 3 cut(s) 365, 592, 843
BstSLI GKGCMC 2 cut(s) 230, 437
BstV1I GCAGC 3 cut(s) 96, 175, 857
Bsu15I ATCGAT 1 cut(s) 406
BsuRI GGCC 2 cut(s) 201, 818
BsuTUI ATCGAT 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 2 cut(s) 637, 674
BtsI GCAGTG 1 cut(s) 1059
BtsIMutI CAGTG 3 cut(s) 406, 484, 1059
BveI ACCTGC 3 cut(s) 358, 372, 983
Cac8I GCNNGC 4 cut(s) 132, 193, 576, 843
CaiI CAGNNNCTG 2 cut(s) 373, 610
CciI TCATGA 1 cut(s) 358
CfoI GCGC 2 cut(s) 130, 1037
Cfr13I GGNCC 6 cut(s) 199, 250, 370, 376, 817, 894
ClaI ATCGAT 1 cut(s) 406
CseI GACGC 2 cut(s) 147, 535
CviAII CATG 4 cut(s) 222, 238, 359, 665
DdeI CTNAG 6 cut(s) 27, 267, 380, 398, 680, 890
DpnI GATC 7 cut(s) 80, 141, 152, 306, 405, 409, 678
DpnII GATC 7 cut(s) 78, 139, 150, 304, 403, 407, 676
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eco130I CCWWGG 2 cut(s) 420, 813
Eco47I GGWCC 4 cut(s) 250, 370, 376, 894
Eco57I CTGAAG 2 cut(s) 77, 305
EcoO109I RGGNCCY 2 cut(s) 370, 894
EcoRII CCWGG 3 cut(s) 123, 372, 869
EcoT14I CCWWGG 2 cut(s) 420, 813
ErhI CCWWGG 2 cut(s) 420, 813
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 4 cut(s) 225, 241, 362, 668
FaiI YATR 7 cut(s) 223, 239, 360, 415, 417, 653, 666
FatI CATG 4 cut(s) 221, 237, 358, 664
FbaI TGATCA 3 cut(s) 78, 139, 150
Fnu4HI GCNGC 6 cut(s) 110, 189, 299, 700, 846, 1006
FokI GGATG 2 cut(s) 644, 681
Fsp4HI GCNGC 6 cut(s) 110, 189, 299, 700, 846, 1006
FspBI CTAG 1 cut(s) 827
GlaI GCGC 2 cut(s) 129, 1036
GluI GCNGC 6 cut(s) 110, 189, 299, 700, 846, 1006
GsuI CTGGAG 2 cut(s) 183, 199
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 2 cut(s) 201, 818
HgaI GACGC 2 cut(s) 147, 535
HhaI GCGC 2 cut(s) 130, 1037
Hin1I GRCGYC 2 cut(s) 158, 1022
Hin1II CATG 4 cut(s) 225, 241, 362, 668
Hin6I GCGC 2 cut(s) 128, 1035
HinP1I GCGC 2 cut(s) 128, 1035
HindIII AAGCTT 1 cut(s) 388
HinfI GANTC 7 cut(s) 165, 286, 532, 613, 823, 857, 953
HphI GGTGA 5 cut(s) 73, 85, 160, 916, 1046
Hpy166II GTNNAC 2 cut(s) 228, 273
Hpy188I TCNGA 6 cut(s) 102, 144, 562, 612, 683, 718
Hpy188III TCNNGA 7 cut(s) 162, 323, 359, 491, 529, 550, 981
Hpy8I GTNNAC 2 cut(s) 228, 273
Hpy99I CGWCG 1 cut(s) 881
HpyAV CCTTC 6 cut(s) 59, 63, 85, 878, 939, 978
HpyCH4III ACNGT 5 cut(s) 148, 593, 688, 746, 782
HpyCH4IV ACGT 1 cut(s) 1022
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 575, 941, 1049
HpyF3I CTNAG 6 cut(s) 27, 267, 380, 398, 680, 890
HpySE526I ACGT 1 cut(s) 1022
Hsp92I GRCGYC 2 cut(s) 158, 1022
Hsp92II CATG 4 cut(s) 225, 241, 362, 668
HspAI GCGC 2 cut(s) 128, 1035
Ksp22I TGATCA 3 cut(s) 78, 139, 150
Kzo9I GATC 7 cut(s) 78, 139, 150, 304, 403, 407, 676
LmnI GCTCC 2 cut(s) 106, 268
Lsp1109I GCAGC 3 cut(s) 96, 175, 857
LweI GCATC 3 cut(s) 175, 566, 659
MaeI CTAG 1 cut(s) 827
MaeII ACGT 1 cut(s) 1022
MaeIII GTNAC 2 cut(s) 479, 587
MalI GATC 7 cut(s) 80, 141, 152, 306, 405, 409, 678
MboI GATC 7 cut(s) 78, 139, 150, 304, 403, 407, 676
MboII GAAGA 2 cut(s) 70, 844
MhlI GDGCHC 3 cut(s) 230, 437, 470
MluCI AATT 1 cut(s) 518
MlyI GAGTC 4 cut(s) 174, 280, 832, 947
MmeI TCCRAC 2 cut(s) 484, 625
MroXI GAANNNNTTC 1 cut(s) 988
MseI TTAA 1 cut(s) 710
MslI CAYNNNNRTG 1 cut(s) 667
MspR9I CCNGG 3 cut(s) 125, 374, 871
Mva1269I GAATGC 2 cut(s) 473, 988
MvaI CCWGG 3 cut(s) 125, 374, 871
MwoI GCNNNNNNNGC 4 cut(s) 115, 575, 941, 1049
NdeII GATC 7 cut(s) 78, 139, 150, 304, 403, 407, 676
NlaIII CATG 4 cut(s) 225, 241, 362, 668
NlaIV GGNNCC 3 cut(s) 264, 434, 896
NmeAIII GCCGAG 1 cut(s) 449
NmuCI GTSAC 1 cut(s) 479
PagI TCATGA 1 cut(s) 358
PaqCI CACCTGC 1 cut(s) 983
PctI GAATGC 2 cut(s) 473, 988
PdmI GAANNNNTTC 1 cut(s) 988
PfeI GAWTC 3 cut(s) 532, 613, 857
PflMI CCANNNNNTGG 1 cut(s) 520
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 6 cut(s) 111, 190, 300, 701, 847, 1007
PleI GAGTC 4 cut(s) 173, 280, 831, 947
PpsI GAGTC 4 cut(s) 173, 280, 831, 947
PpuMI RGGWCCY 2 cut(s) 370, 894
Psp5II RGGWCCY 2 cut(s) 370, 894
Psp6I CCWGG 3 cut(s) 123, 372, 869
PspGI CCWGG 3 cut(s) 123, 372, 869
PspN4I GGNNCC 3 cut(s) 264, 434, 896
PspPI GGNCC 6 cut(s) 199, 250, 370, 376, 817, 894
PspPPI RGGWCCY 2 cut(s) 370, 894
PstI CTGCAG 2 cut(s) 369, 847
PstNI CAGNNNCTG 2 cut(s) 373, 610
RseI CAYNNNNRTG 1 cut(s) 667
SaqAI TTAA 1 cut(s) 710
SatI GCNGC 6 cut(s) 110, 189, 299, 700, 846, 1006
Sau3AI GATC 7 cut(s) 78, 139, 150, 304, 403, 407, 676
Sau96I GGNCC 6 cut(s) 199, 250, 370, 376, 817, 894
SbfI CCTGCAGG 1 cut(s) 369
SchI GAGTC 4 cut(s) 174, 280, 832, 947
ScrFI CCNGG 3 cut(s) 125, 374, 871
SdaI CCTGCAGG 1 cut(s) 369
SduI GDGCHC 3 cut(s) 230, 437, 470
SfaNI GCATC 3 cut(s) 175, 566, 659
SfcI CTRYAG 3 cut(s) 365, 592, 843
SinI GGWCC 4 cut(s) 250, 370, 376, 894
SmiMI CAYNNNNRTG 1 cut(s) 667
Sse8387I CCTGCAGG 1 cut(s) 369
Sse9I AATT 1 cut(s) 518
SsiI CCGC 5 cut(s) 298, 699, 944, 1005, 1057
SspMI CTAG 1 cut(s) 827
StyD4I CCNGG 3 cut(s) 123, 372, 869
StyI CCWWGG 2 cut(s) 420, 813
TaaI ACNGT 5 cut(s) 148, 593, 688, 746, 782
TaiI ACGT 1 cut(s) 1025
TaqI TCGA 1 cut(s) 406
TasI AATT 1 cut(s) 518
TauI GCSGC 3 cut(s) 301, 702, 1008
TfiI GAWTC 3 cut(s) 532, 613, 857
Tru1I TTAA 1 cut(s) 710
Tru9I TTAA 1 cut(s) 710
TscAI CASTG 3 cut(s) 406, 484, 1066
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 3 cut(s) 109, 188, 845
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 1 cut(s) 254
TspGWI ACGGA 1 cut(s) 1015
TspRI CASTG 3 cut(s) 406, 484, 1066
Van91I CCANNNNNTGG 1 cut(s) 520
VneI GTGCAC 1 cut(s) 226
VpaK11BI GGWCC 4 cut(s) 250, 370, 376, 894
XmnI GAANNNNTTC 1 cut(s) 988
XspI CTAG 1 cut(s) 827
ZraI GACGTC 1 cut(s) 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.