MD05G1010400.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005717
Physical Location & Seq
Forward (+)
2128365 .. 2132253
3889 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1010400.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGGTGGGAAAAGGAAGCATCAAGGCTGACGACTTGACCTTGTGCTTTGAGAAGCTTATGATGTTTGCAAGTGCAAGTGGAAGTGCTTCTGGTGGAAGTGCTTCTGTTAGTGGTAGTAGTGGTGGTAGTGGTGGTAGTGGTGGGAAAATGAAGATGGAAGGAAGGGTGATGATCACTGATTGGAAGGATATTCCGATAGAGCTGCTTCTGCAAATCCTGGCGCTTACGGATGATCAGACGGTGATCCCGGCGTCTGGAGTCTGCCGTGGCTGGAGAGATGCTGTTTGCTTGGGCCTTACTCGCCTCTCCCTCTCATGGTGCACAAAGAACATGAATAGCTTGGTCCTATCTCTAGCTCCTAAGTTTACGAGATTGCAGACTCTAATACTGCGTCAAGATCATAAACCCCAGCTTGAGGATAGTGCTGTTGAGAGCATTGCAAACTTCTGTCATGACCTGCAGGTCCTGGACCTCAGCAAAAGCTTCAAGCTCAGTGACCGATCCCTGTATGCCTTAGCACATGGGTGCCCAAATCTTACAAAACTAAACATTAGTGGGTGCTCGGCATTCAGTGACATTGCTCTTGAATATCTTGCTGGCTTTTGCCGAAAATTGGAAGTCTTGAATCTTTGCGGTTGTGTCAGGACTGCATCAGACAGGGCATTGCAGGCTATTGGGCGTTACTGTAGTCAGTTGCAGTCTCTGAATCTTGGTTGGTGTGAGGATGTAAGTGATGTTGGAGTTATGAGTTTGGCATACGGATGCCCTGATCTCCGAACTGTTGATTTGTGTGGCTGTGTCCTAATATCAGATGACAGTGTGATTGCTTTGGCAAATAGGTGTCCTCATTTGCAATCGCTTGGCCTTTACTACTGTAAGAACATTACAGACAGAGCAATGTATTCCTTGGCCCAGAGTCTAGTGAAGAACAAGCCTGCGGCAATGTGGGAATCCCGGAAAACCAGGAATGACGAGGAAGGGCTTAGGACTCTCAACATCAGCCAATGCACAGCGCTCACCCCTTCTGCTGTTCAGGCTGTGTGCGACTCATTCCCTGCCCTTCACACCTGCTCTGGAAGGCATTCCCTCAACTTTAGCGGCTGTATGAATTTGACGTCAGTGCATTGTGCCTGTGCTGTCCAAGCTCACCGCACCGCCAACGCCTTTCCTCATTCAGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009987 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010286 GO:0010311 GO:0010498 GO:0016567 GO:0016740 GO:0019005 GO:0019538 GO:0019787 GO:0019941 GO:0022622 GO:0030163 GO:0031146 GO:0031461 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0036211 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048364 GO:0048518 GO:0048519 GO:0048522 GO:0048527 GO:0048528 GO:0048580 GO:0048581 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051302 GO:0051603 GO:0051716 GO:0051781 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140096 GO:1901332 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905392 GO:1905393 GO:1990234 GO:2000023 GO:2000026 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04068 ko04110 ko04120 ko04150 ko05168 ko05169 ko05200 ko05203 ko05222 map04068 map04110 map04120 map04150 map05168 map05169 map05200 map05203 map05222
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

395

Amino Acids

42.35

Weight (kDa)

7.74

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 61 - 96 6.6e-06 F-box-like
DUF7885 PF25372 97 - 306 4.7e-16 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1076
AasI GACNNNNNNGTC 1 cut(s) 461
AatII GACGTC 1 cut(s) 1118
Acc36I ACCTGC 3 cut(s) 451, 465, 1076
AccB1I GGYRCC 1 cut(s) 525
AciI CCGC 5 cut(s) 633, 938, 1098, 1150, 1155
AclWI GGATC 2 cut(s) 238, 495
AcsI RAATTY 1 cut(s) 1108
AcyI GRCGYC 2 cut(s) 251, 1115
AfeI AGCGCT 1 cut(s) 1014
AfiI CCNNNNNNNGG 4 cut(s) 254, 315, 415, 613
AgsI TTSAA 3 cut(s) 487, 587, 625
AjnI CCWGG 3 cut(s) 216, 465, 962
AleI CACNNNNGTG 1 cut(s) 523
AluBI AGCT 9 cut(s) 55, 202, 339, 356, 412, 483, 490, 1145, 1178
AluI AGCT 9 cut(s) 55, 202, 339, 356, 412, 483, 490, 1145, 1178
Alw21I GWGCWC 2 cut(s) 323, 563
Alw26I GTCTC 1 cut(s) 705
Alw44I GTGCAC 1 cut(s) 319
AlwI GGATC 2 cut(s) 238, 495
AlwNI CAGNNNCTG 2 cut(s) 466, 703
Aor51HI AGCGCT 1 cut(s) 1014
AoxI GGCC 3 cut(s) 292, 862, 909
ApaLI GTGCAC 1 cut(s) 319
ApeKI GCWGC 1 cut(s) 202
ApoI RAATTY 1 cut(s) 1108
Asp700I GAANNNNTTC 3 cut(s) 85, 100, 1081
AspLEI GCGC 2 cut(s) 223, 1015
AspS9I GGNCC 5 cut(s) 292, 343, 463, 469, 910
AsuC2I CCSGG 2 cut(s) 248, 955
AsuHPI GGTGA 4 cut(s) 178, 253, 1009, 1139
AvaII GGWCC 3 cut(s) 343, 463, 469
BaeGI GKGCMC 2 cut(s) 323, 530
BanI GGYRCC 1 cut(s) 525
Bbv12I GWGCWC 2 cut(s) 323, 563
BbvCI CCTCAGC 1 cut(s) 473
BbvI GCAGC 1 cut(s) 189
BccI CCATC 1 cut(s) 148
BceAI ACGGC 1 cut(s) 249
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BciT130I CCWGG 3 cut(s) 218, 467, 964
BclI TGATCA 2 cut(s) 171, 232
BcnI CCSGG 2 cut(s) 248, 955
BcoDI GTCTC 1 cut(s) 705
BfaI CTAG 2 cut(s) 353, 920
BfmI CTRYAG 2 cut(s) 458, 685
BfoI RGCGCY 2 cut(s) 224, 1016
BfuAI ACCTGC 3 cut(s) 451, 465, 1076
BisI GCNGC 3 cut(s) 203, 939, 1099
BlsI GCNGC 3 cut(s) 204, 940, 1100
Bme1390I CCNGG 5 cut(s) 218, 248, 467, 955, 964
Bme18I GGWCC 3 cut(s) 343, 463, 469
BmgT120I GGNCC 5 cut(s) 292, 343, 463, 469, 910
BmiI GGNNCC 1 cut(s) 527
BmrFI CCNGG 5 cut(s) 218, 248, 467, 955, 964
BmsI GCATC 4 cut(s) 27, 268, 659, 752
BpmI CTGGAG 2 cut(s) 276, 292
Bpu10I CCTNAGC 3 cut(s) 473, 514, 983
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 2 cut(s) 248, 955
BsaHI GRCGYC 2 cut(s) 251, 1115
BsaJI CCNNGG 2 cut(s) 265, 906
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 315, 415, 613
Bse3DI GCAATG 5 cut(s) 435, 576, 662, 903, 948
BseBI CCWGG 3 cut(s) 218, 467, 964
BseDI CCNNGG 2 cut(s) 265, 906
BseGI GGATG 3 cut(s) 235, 730, 767
BseLI CCNNNNNNNGG 4 cut(s) 254, 315, 415, 613
BseMI GCAATG 5 cut(s) 435, 576, 662, 903, 948
BseMII CTCAG 2 cut(s) 487, 505
BseSI GKGCMC 2 cut(s) 323, 530
BseXI GCAGC 1 cut(s) 189
BseYI CCCAGC 1 cut(s) 408
BshFI GGCC 3 cut(s) 294, 864, 911
BshNI GGYRCC 1 cut(s) 525
BsiHKAI GWGCWC 2 cut(s) 323, 563
BsiSI CCGG 2 cut(s) 248, 955
BslI CCNNNNNNNGG 4 cut(s) 254, 315, 415, 613
BsmAI GTCTC 1 cut(s) 705
BsmI GAATGC 2 cut(s) 566, 1081
BsnI GGCC 3 cut(s) 294, 864, 911
Bsp1286I GDGCHC 3 cut(s) 323, 530, 563
Bsp143I GATC 6 cut(s) 171, 232, 243, 397, 500, 769
BspACI CCGC 5 cut(s) 633, 938, 1098, 1150, 1155
BspANI GGCC 3 cut(s) 294, 864, 911
BspCNI CTCAG 2 cut(s) 486, 504
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 1 cut(s) 527
BspMAI CTGCAG 1 cut(s) 462
BspMI ACCTGC 3 cut(s) 451, 465, 1076
BspPI GGATC 2 cut(s) 238, 495
BspT107I GGYRCC 1 cut(s) 525
BsrDI GCAATG 5 cut(s) 435, 576, 662, 903, 948
BssECI CCNNGG 2 cut(s) 265, 906
BssMI GATC 6 cut(s) 171, 232, 243, 397, 500, 769
BssNI GRCGYC 2 cut(s) 251, 1115
BssT1I CCWWGG 1 cut(s) 906
Bst2UI CCWGG 3 cut(s) 218, 467, 964
Bst4CI ACNGT 5 cut(s) 241, 686, 781, 818, 875
BstACI GRCGYC 2 cut(s) 251, 1115
BstC8I GCNNGC 3 cut(s) 598, 669, 936
BstDEI CTNAG 5 cut(s) 360, 473, 491, 514, 983
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 3 cut(s) 235, 730, 767
BstH2I RGCGCY 2 cut(s) 224, 1016
BstHHI GCGC 2 cut(s) 223, 1015
BstKTI GATC 6 cut(s) 174, 235, 246, 400, 503, 772
BstMAI GTCTC 1 cut(s) 705
BstMBI GATC 6 cut(s) 171, 232, 243, 397, 500, 769
BstMWI GCNNNNNNNGC 5 cut(s) 208, 300, 668, 1034, 1142
BstNI CCWGG 3 cut(s) 218, 467, 964
BstSCI CCNGG 5 cut(s) 216, 246, 465, 953, 962
BstSFI CTRYAG 2 cut(s) 458, 685
BstSLI GKGCMC 2 cut(s) 323, 530
BstV1I GCAGC 1 cut(s) 189
BsuRI GGCC 3 cut(s) 294, 864, 911
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 3 cut(s) 235, 730, 767
BtsIMutI CAGTG 5 cut(s) 174, 499, 577, 823, 1125
BveI ACCTGC 3 cut(s) 451, 465, 1076
Cac8I GCNNGC 3 cut(s) 598, 669, 936
CaiI CAGNNNCTG 2 cut(s) 466, 703
CciI TCATGA 1 cut(s) 451
CfoI GCGC 2 cut(s) 223, 1015
Cfr13I GGNCC 5 cut(s) 292, 343, 463, 469, 910
CseI GACGC 2 cut(s) 240, 380
CviAII CATG 4 cut(s) 315, 331, 452, 521
DdeI CTNAG 5 cut(s) 360, 473, 491, 514, 983
DpnI GATC 6 cut(s) 173, 234, 245, 399, 502, 771
DpnII GATC 6 cut(s) 171, 232, 243, 397, 500, 769
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
Eco130I CCWWGG 1 cut(s) 906
Eco47I GGWCC 3 cut(s) 343, 463, 469
Eco47III AGCGCT 1 cut(s) 1014
EcoO109I RGGNCCY 1 cut(s) 463
EcoRII CCWGG 3 cut(s) 216, 465, 962
EcoT14I CCWWGG 1 cut(s) 906
ErhI CCWWGG 1 cut(s) 906
FaeI CATG 4 cut(s) 318, 334, 455, 524
FatI CATG 4 cut(s) 314, 330, 451, 520
FbaI TGATCA 2 cut(s) 171, 232
Fnu4HI GCNGC 3 cut(s) 203, 939, 1099
FokI GGATG 3 cut(s) 242, 737, 774
Fsp4HI GCNGC 3 cut(s) 203, 939, 1099
FspBI CTAG 2 cut(s) 353, 920
GlaI GCGC 2 cut(s) 222, 1014
GluI GCNGC 3 cut(s) 203, 939, 1099
GsaI CCCAGC 1 cut(s) 412
GsuI CTGGAG 2 cut(s) 276, 292
HaeII RGCGCY 2 cut(s) 224, 1016
HaeIII GGCC 3 cut(s) 294, 864, 911
HapII CCGG 2 cut(s) 248, 955
HgaI GACGC 2 cut(s) 240, 380
HhaI GCGC 2 cut(s) 223, 1015
Hin1I GRCGYC 2 cut(s) 251, 1115
Hin1II CATG 4 cut(s) 318, 334, 455, 524
Hin6I GCGC 2 cut(s) 221, 1013
HinP1I GCGC 2 cut(s) 221, 1013
HindIII AAGCTT 2 cut(s) 53, 481
HinfI GANTC 8 cut(s) 258, 379, 625, 706, 916, 950, 988, 1046
HpaII CCGG 2 cut(s) 248, 955
HphI GGTGA 4 cut(s) 178, 253, 1009, 1139
Hpy166II GTNNAC 2 cut(s) 321, 366
Hpy188I TCNGA 6 cut(s) 195, 237, 655, 705, 776, 811
Hpy188III TCNNGA 7 cut(s) 255, 395, 452, 584, 622, 643, 1074
Hpy8I GTNNAC 2 cut(s) 321, 366
HpyAV CCTTC 7 cut(s) 152, 156, 178, 971, 1032, 1070, 1071
HpyCH4III ACNGT 5 cut(s) 241, 686, 781, 818, 875
HpyCH4IV ACGT 1 cut(s) 1115
HpyF10VI GCNNNNNNNGC 5 cut(s) 208, 300, 668, 1034, 1142
HpyF3I CTNAG 5 cut(s) 360, 473, 491, 514, 983
HpySE526I ACGT 1 cut(s) 1115
Hsp92I GRCGYC 2 cut(s) 251, 1115
Hsp92II CATG 4 cut(s) 318, 334, 455, 524
HspAI GCGC 2 cut(s) 221, 1013
Ksp22I TGATCA 2 cut(s) 171, 232
Kzo9I GATC 6 cut(s) 171, 232, 243, 397, 500, 769
LmnI GCTCC 1 cut(s) 361
Lsp1109I GCAGC 1 cut(s) 189
LweI GCATC 4 cut(s) 27, 268, 659, 752
MaeI CTAG 2 cut(s) 353, 920
MaeII ACGT 1 cut(s) 1115
MaeIII GTNAC 3 cut(s) 494, 572, 680
MalI GATC 6 cut(s) 173, 234, 245, 399, 502, 771
MboI GATC 6 cut(s) 171, 232, 243, 397, 500, 769
MboII GAAGA 2 cut(s) 163, 937
MhlI GDGCHC 3 cut(s) 323, 530, 563
MluCI AATT 2 cut(s) 611, 1108
MlyI GAGTC 5 cut(s) 267, 373, 925, 982, 1040
MmeI TCCRAC 1 cut(s) 718
MnlI CCTC 9 cut(s) 314, 320, 409, 482, 715, 855, 967, 1097, 1179
MroXI GAANNNNTTC 3 cut(s) 85, 100, 1081
MslI CAYNNNNRTG 2 cut(s) 523, 760
MspI CCGG 2 cut(s) 248, 955
MspR9I CCNGG 5 cut(s) 218, 248, 467, 955, 964
Mva1269I GAATGC 2 cut(s) 566, 1081
MvaI CCWGG 3 cut(s) 218, 467, 964
MwoI GCNNNNNNNGC 5 cut(s) 208, 300, 668, 1034, 1142
NciI CCSGG 2 cut(s) 248, 955
NdeII GATC 6 cut(s) 171, 232, 243, 397, 500, 769
NlaIII CATG 4 cut(s) 318, 334, 455, 524
NlaIV GGNNCC 1 cut(s) 527
NmeAIII GCCGAG 1 cut(s) 542
NmuCI GTSAC 2 cut(s) 494, 572
OliI CACNNNNGTG 1 cut(s) 523
PagI TCATGA 1 cut(s) 451
PaqCI CACCTGC 1 cut(s) 1076
PctI GAATGC 2 cut(s) 566, 1081
PdmI GAANNNNTTC 3 cut(s) 85, 100, 1081
PfeI GAWTC 3 cut(s) 625, 706, 950
PfoI TCCNGGA 2 cut(s) 465, 953
PkrI GCNGC 3 cut(s) 204, 940, 1100
PleI GAGTC 5 cut(s) 266, 373, 924, 982, 1040
PpsI GAGTC 5 cut(s) 266, 373, 924, 982, 1040
PpuMI RGGWCCY 1 cut(s) 463
Psp5II RGGWCCY 1 cut(s) 463
Psp6I CCWGG 3 cut(s) 216, 465, 962
PspFI CCCAGC 1 cut(s) 408
PspGI CCWGG 3 cut(s) 216, 465, 962
PspN4I GGNNCC 1 cut(s) 527
PspPI GGNCC 5 cut(s) 292, 343, 463, 469, 910
PspPPI RGGWCCY 1 cut(s) 463
PstI CTGCAG 1 cut(s) 462
PstNI CAGNNNCTG 2 cut(s) 466, 703
RseI CAYNNNNRTG 2 cut(s) 523, 760
SatI GCNGC 3 cut(s) 203, 939, 1099
Sau3AI GATC 6 cut(s) 171, 232, 243, 397, 500, 769
Sau96I GGNCC 5 cut(s) 292, 343, 463, 469, 910
SbfI CCTGCAGG 1 cut(s) 462
SchI GAGTC 5 cut(s) 267, 373, 925, 982, 1040
ScrFI CCNGG 5 cut(s) 218, 248, 467, 955, 964
SdaI CCTGCAGG 1 cut(s) 462
SduI GDGCHC 3 cut(s) 323, 530, 563
SfaNI GCATC 4 cut(s) 27, 268, 659, 752
SfcI CTRYAG 2 cut(s) 458, 685
SinI GGWCC 3 cut(s) 343, 463, 469
SmiMI CAYNNNNRTG 2 cut(s) 523, 760
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse8387I CCTGCAGG 1 cut(s) 462
Sse9I AATT 2 cut(s) 611, 1108
SsiI CCGC 5 cut(s) 633, 938, 1098, 1150, 1155
SspMI CTAG 2 cut(s) 353, 920
StyD4I CCNGG 5 cut(s) 216, 246, 465, 953, 962
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 5 cut(s) 241, 686, 781, 818, 875
TaiI ACGT 1 cut(s) 1118
TaqII GACCGA 1 cut(s) 513
TasI AATT 2 cut(s) 611, 1108
TauI GCSGC 2 cut(s) 941, 1101
TfiI GAWTC 3 cut(s) 625, 706, 950
TscAI CASTG 5 cut(s) 181, 499, 577, 823, 1125
TseFI GTSAC 2 cut(s) 494, 572
TseI GCWGC 1 cut(s) 202
Tsp45I GTSAC 2 cut(s) 494, 572
TspDTI ATGAA 3 cut(s) 164, 347, 1121
TspGWI ACGGA 2 cut(s) 242, 774
TspRI CASTG 5 cut(s) 181, 499, 577, 823, 1125
VneI GTGCAC 1 cut(s) 319
VpaK11BI GGWCC 3 cut(s) 343, 463, 469
XapI RAATTY 1 cut(s) 1108
XmnI GAANNNNTTC 3 cut(s) 85, 100, 1081
XspI CTAG 2 cut(s) 353, 920
ZraI GACGTC 1 cut(s) 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.