AT1G80770

PDE318 PDE318 (pigment defective 318)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005154
Physical Location & Seq
Forward (+)
30355025 .. 30357988
2964 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G80770.6

Sequence Viewer

Length: 1356 bp
ATGAAATTGGCCTCGAGCTTGGTTCCTCATCAATGGCGTCTCTTAACATCACCACAGCTTTCTCTACAAGCTTTCATTTTCTCCACAGCCTCCACCACAAAGTCTCACACTTTCTGGTCTCCAAGCTTGCGTCAATGTCGGAATCTTCAGACGGCTCTTTCTCCAATCGTCAGTTCTAGCTACTTGCCCACTTCCTACATCACTCAGAAGCAGATTGAAATCCCTACCTCCCCTGAAAAACAATCGCCACCAGTGCAGGAAGGTTTGGGTGCTTTTCAAAAACTGCCCATGGTCATGCCATCTATTGATTTGTATGCTTCTGCTCTCAGGAAGTCTAAAAGAGTCCAACCAACTAAAGGCATTGCTAATATTGCTAAGCGAGAAAGAAATAGAGGTGCTAAACAGCTTGATGCATTCATGAAAGAACTGGCTCTACCTTTAAAAGGCTACATGGAAAGTTTTCCCAGGAAGAAACTCTTGCATCCATATGAAAGGTCTCTTATTGACTTGACACTTGGTGATGGAAAGTATGAAGAGGTGTTAGGAAAAGTTGATGTTCTGAGGAAGAAGGTGCAATCTGTTGGAAAGGAACATGCTTCTCTCTGCGCCAAGGCTTTGTCAAAGAAGGAGGCAGAGGAACGCTTGAGCGAAGGTGTGGAGAAGCTGGAATTGGTTTTCCAACAACAAGGGGGAGCTGTTGATGATTTGCTAACTATAGCGAAGGTTTTGAGGGCTATGCCAGTTGTTGACTTGGAAATGCCGACTCTTTGCCTTGTCGGAGCACCAAACGTTGGGAAGTCATCATTGGTTCGCATACTGTCAACAGGGAAGCCTGAGATTTGCAATTATCCTTTCACAACCAGAGGAATTCTGATGGGTCACATCGTTTTAAACTACCAACGATTTCAGGTTACAGACACCCCTGGCCTTCTTAGGAGATGCGATGAGGATAGGAATAATCTAGAGAAGTTAACTCTTGCTGTACTCACTCATCTTCCAACCGCGGTTCTATATGTTCATGATCTAACGGGAGAATGTGGGACTTCTCCTTCTGATCAGTTTCAAATATATAAAGAGATGAAAGAGAGGTTTAAGGATTACCTGTGGATTGATGCTGTGTCCAAATGCGATCTGCTGGGAGGCTCACCGGTGATGTATGCCAAAGAAGATAGAAGCAGTGATGACGCGGAAATCATTAAGTACCGGGAAAGAGGACCTGATGAATCAATTCATGTCTCGGTGAAAACAGAACAAGGTCTCAATGAGCTAAAGAACAAAGTGAAGGAAGTACTGAGTAGTGAGATGGAGAAGATCCAAAGTGGAGAGAAAACTGATCAAAGTGTTGCTACTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1 NOG1

Protein Analysis

451

Amino Acids

50.41

Weight (kDa)

8.87

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOG1_N PF17835 123 - 281 5.1e-42 NOG1 N-terminal helical domain
FeoB_N PF02421 286 - 343 3.5e-08 Ferrous iron transport protein B
MMR_HSR1 PF01926 287 - 381 1.8e-15 50S ribosome-binding GTPase
NOG1 PF06858 352 - 408 2.8e-17 Nucleolar GTP-binding protein 1 (NOG1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 791
AccII CGCG 2 cut(s) 1004, 1187
AciI CCGC 3 cut(s) 1002, 1004, 1187
AclI AACGTT 1 cut(s) 789
AclWI GGATC 1 cut(s) 1306
AcsI RAATTY 1 cut(s) 867
AcuI CTGAAG 1 cut(s) 131
AcyI GRCGYC 1 cut(s) 37
AdeI CACNNNGTG 1 cut(s) 518
AfaI GTAC 3 cut(s) 984, 1202, 1290
AfiI CCNNNNNNNGG 3 cut(s) 356, 443, 791
AgeI ACCGGT 1 cut(s) 1147
AgsI TTSAA 3 cut(s) 218, 278, 1064
AjnI CCWGG 2 cut(s) 464, 922
AjuI GAANNNNNNNTTGG 6 cut(s) 157, 189, 653, 685, 788, 820
AluBI AGCT 9 cut(s) 18, 58, 71, 126, 180, 406, 664, 695, 1267
AluI AGCT 9 cut(s) 18, 58, 71, 126, 180, 406, 664, 695, 1267
Alw21I GWGCWC 1 cut(s) 784
Alw26I GTCTC 6 cut(s) 44, 108, 123, 501, 1240, 1262
AlwI GGATC 1 cut(s) 1306
Ama87I CYCGRG 1 cut(s) 13
AoxI GGCC 2 cut(s) 9, 925
ApoI RAATTY 1 cut(s) 867
AsiGI ACCGGT 1 cut(s) 1147
Asp700I GAANNNNTTC 2 cut(s) 459, 1227
AspLEI GCGC 1 cut(s) 608
AspS9I GGNCC 1 cut(s) 1214
AsuC2I CCSGG 1 cut(s) 1205
AsuHPI GGTGA 5 cut(s) 42, 530, 1137, 1162, 1252
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 1 cut(s) 1214
Bbv12I GWGCWC 1 cut(s) 784
BccI CCATC 4 cut(s) 307, 515, 868, 1297
BceAI ACGGC 1 cut(s) 168
BciT130I CCWGG 2 cut(s) 466, 924
BclI TGATCA 2 cut(s) 1054, 1333
BcnI CCSGG 1 cut(s) 1205
BcoDI GTCTC 6 cut(s) 44, 108, 123, 501, 1240, 1262
BfaI CTAG 3 cut(s) 177, 962, 1354
BfmI CTRYAG 1 cut(s) 714
BlpI GCTNAGC 1 cut(s) 375
BmcAI AGTACT 1 cut(s) 1290
Bme1390I CCNGG 3 cut(s) 466, 924, 1205
Bme18I GGWCC 1 cut(s) 1214
BmeT110I CYCGRG 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 1214
BmiI GGNNCC 1 cut(s) 24
BmrFI CCNGG 3 cut(s) 466, 924, 1205
BmsI GCATC 4 cut(s) 400, 490, 929, 1102
Bpu1102I GCTNAGC 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 664
BpuMI CCSGG 1 cut(s) 1205
BsaBI GATNNNNATC 1 cut(s) 218
BsaHI GRCGYC 1 cut(s) 37
BsaI GGTCTC 3 cut(s) 123, 501, 1262
BsaJI CCNNGG 5 cut(s) 288, 464, 609, 922, 1002
BsaWI WCCGGW 1 cut(s) 1147
Bsc4I CCNNNNNNNGG 3 cut(s) 356, 443, 791
Bse118I RCCGGY 1 cut(s) 1147
Bse1I ACTGG 3 cut(s) 251, 432, 740
Bse3DI GCAATG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 218
BseBI CCWGG 2 cut(s) 466, 924
BseDI CCNNGG 5 cut(s) 288, 464, 609, 922, 1002
BseGI GGATG 1 cut(s) 481
BseJI GATNNNNATC 1 cut(s) 218
BseLI CCNNNNNNNGG 3 cut(s) 356, 443, 791
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 5 cut(s) 218, 340, 551, 825, 1283
BseNI ACTGG 3 cut(s) 251, 432, 740
BseYI CCCAGC 1 cut(s) 1135
BsgI GTGCAG 1 cut(s) 275
Bsh1236I CGCG 2 cut(s) 1004, 1187
BshFI GGCC 2 cut(s) 11, 927
BshTI ACCGGT 1 cut(s) 1147
BsiHKAI GWGCWC 1 cut(s) 784
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 2 cut(s) 1148, 1204
BslFI GGGAC 1 cut(s) 1054
BslI CCNNNNNNNGG 3 cut(s) 356, 443, 791
BsmAI GTCTC 6 cut(s) 44, 108, 123, 501, 1240, 1262
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 1054
BsmI GAATGC 1 cut(s) 413
BsnI GGCC 2 cut(s) 11, 927
Bso31I GGTCTC 3 cut(s) 123, 501, 1262
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 784
Bsp143I GATC 5 cut(s) 1021, 1054, 1129, 1311, 1333
Bsp1720I GCTNAGC 1 cut(s) 375
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 3 cut(s) 1002, 1004, 1187
BspANI GGCC 2 cut(s) 11, 927
BspCNI CTCAG 5 cut(s) 217, 339, 552, 826, 1284
BspFNI CGCG 2 cut(s) 1004, 1187
BspHI TCATGA 2 cut(s) 417, 1018
BspLI GGNNCC 1 cut(s) 24
BspPI GGATC 1 cut(s) 1306
BspTNI GGTCTC 3 cut(s) 123, 501, 1262
BsrDI GCAATG 1 cut(s) 360
BsrFI RCCGGY 1 cut(s) 1147
BsrI ACTGG 3 cut(s) 251, 432, 740
BssAI RCCGGY 1 cut(s) 1147
BssECI CCNNGG 5 cut(s) 288, 464, 609, 922, 1002
BssMI GATC 5 cut(s) 1021, 1054, 1129, 1311, 1333
BssNI GRCGYC 1 cut(s) 37
BssT1I CCWWGG 2 cut(s) 288, 609
Bst2UI CCWGG 2 cut(s) 466, 924
Bst4CI ACNGT 1 cut(s) 819
Bst6I CTCTTC 1 cut(s) 528
BstACI GRCGYC 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 128
BstDEI CTNAG 7 cut(s) 204, 326, 375, 560, 834, 932, 1292
BstDSI CCRYGG 2 cut(s) 288, 1002
BstENI CCTNNNNNAGG 1 cut(s) 441
BstF5I GGATG 1 cut(s) 481
BstFNI CGCG 2 cut(s) 1004, 1187
BstHHI GCGC 1 cut(s) 608
BstKTI GATC 5 cut(s) 1024, 1057, 1132, 1314, 1336
BstMAI GTCTC 6 cut(s) 44, 108, 123, 501, 1240, 1262
BstMBI GATC 5 cut(s) 1021, 1054, 1129, 1311, 1333
BstMWI GCNNNNNNNGC 2 cut(s) 253, 371
BstNI CCWGG 2 cut(s) 466, 924
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 3 cut(s) 464, 922, 1203
BstSFI CTRYAG 1 cut(s) 714
BstUI CGCG 2 cut(s) 1004, 1187
BstX2I RGATCY 1 cut(s) 1311
BstYI RGATCY 1 cut(s) 1311
BsuRI GGCC 2 cut(s) 11, 927
BtgI CCRYGG 2 cut(s) 288, 1002
BtgZI GCGATG 1 cut(s) 957
BtsCI GGATG 1 cut(s) 481
BtsI GCAGTG 1 cut(s) 1183
BtsIMutI CAGTG 2 cut(s) 258, 1183
Cac8I GCNNGC 1 cut(s) 128
CciI TCATGA 2 cut(s) 417, 1018
CfoI GCGC 1 cut(s) 608
Cfr10I RCCGGY 1 cut(s) 1147
Cfr13I GGNCC 1 cut(s) 1214
Cfr42I CCGCGG 1 cut(s) 1005
CseI GACGC 3 cut(s) 26, 119, 1193
Csp6I GTAC 3 cut(s) 983, 1201, 1289
CspAI ACCGGT 1 cut(s) 1147
CviAII CATG 7 cut(s) 289, 295, 418, 451, 593, 1019, 1232
CviQI GTAC 3 cut(s) 983, 1201, 1289
DdeI CTNAG 7 cut(s) 204, 326, 375, 560, 834, 932, 1292
DpnI GATC 5 cut(s) 1023, 1056, 1131, 1313, 1335
DpnII GATC 5 cut(s) 1021, 1054, 1129, 1311, 1333
DraI TTTAAA 2 cut(s) 441, 891
DraIII CACNNNGTG 1 cut(s) 518
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
Eco130I CCWWGG 2 cut(s) 288, 609
Eco31I GGTCTC 3 cut(s) 123, 501, 1262
Eco47I GGWCC 1 cut(s) 1214
Eco57I CTGAAG 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 13
EcoNI CCTNNNNNAGG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 1214
EcoRI GAATTC 1 cut(s) 867
EcoRII CCWGG 2 cut(s) 464, 922
EcoT14I CCWWGG 2 cut(s) 288, 609
EcoT22I ATGCAT 1 cut(s) 415
ErhI CCWWGG 2 cut(s) 288, 609
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 7 cut(s) 292, 298, 421, 454, 596, 1022, 1235
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 1 cut(s) 1054
FatI CATG 7 cut(s) 288, 294, 417, 450, 592, 1018, 1231
FauNDI CATATG 1 cut(s) 487
FbaI TGATCA 2 cut(s) 1054, 1333
FokI GGATG 1 cut(s) 468
FspBI CTAG 3 cut(s) 177, 962, 1354
GlaI GCGC 1 cut(s) 607
GsaI CCCAGC 1 cut(s) 1139
HaeIII GGCC 2 cut(s) 11, 927
HapII CCGG 2 cut(s) 1148, 1204
HgaI GACGC 3 cut(s) 26, 119, 1193
HhaI GCGC 1 cut(s) 608
Hin1I GRCGYC 1 cut(s) 37
Hin1II CATG 7 cut(s) 292, 298, 421, 454, 596, 1022, 1235
Hin6I GCGC 1 cut(s) 606
HinP1I GCGC 1 cut(s) 606
HincII GTYRAC 3 cut(s) 748, 822, 972
HindII GTYRAC 3 cut(s) 748, 822, 972
HindIII AAGCTT 2 cut(s) 69, 124
HinfI GANTC 4 cut(s) 142, 342, 763, 1223
HpaI GTTAAC 1 cut(s) 972
HpaII CCGG 2 cut(s) 1148, 1204
HphI GGTGA 5 cut(s) 42, 530, 1137, 1162, 1252
Hpy166II GTNNAC 3 cut(s) 748, 822, 972
Hpy188I TCNGA 7 cut(s) 141, 150, 207, 561, 779, 873, 1054
Hpy188III TCNNGA 4 cut(s) 328, 418, 962, 1019
Hpy8I GTNNAC 3 cut(s) 748, 822, 972
HpyAV CCTTC 8 cut(s) 254, 562, 619, 644, 715, 938, 1059, 1276
HpyCH4III ACNGT 1 cut(s) 819
HpyCH4IV ACGT 1 cut(s) 789
HpyCH4V TGCA 5 cut(s) 256, 413, 481, 574, 843
HpyF10VI GCNNNNNNNGC 2 cut(s) 253, 371
HpyF3I CTNAG 7 cut(s) 204, 326, 375, 560, 834, 932, 1292
HpySE526I ACGT 1 cut(s) 789
Hsp92I GRCGYC 1 cut(s) 37
Hsp92II CATG 7 cut(s) 292, 298, 421, 454, 596, 1022, 1235
HspAI GCGC 1 cut(s) 606
Ksp22I TGATCA 2 cut(s) 1054, 1333
KspAI GTTAAC 1 cut(s) 972
KspI CCGCGG 1 cut(s) 1005
Kzo9I GATC 5 cut(s) 1021, 1054, 1129, 1311, 1333
LmnI GCTCC 2 cut(s) 692, 779
LweI GCATC 4 cut(s) 400, 490, 929, 1102
MaeI CTAG 3 cut(s) 177, 962, 1354
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 2 cut(s) 878, 910
MalI GATC 5 cut(s) 1023, 1056, 1131, 1313, 1335
MboI GATC 5 cut(s) 1021, 1054, 1129, 1311, 1333
MboII GAAGA 7 cut(s) 137, 481, 545, 577, 986, 1178, 1321
MflI RGATCY 1 cut(s) 1311
MhlI GDGCHC 1 cut(s) 784
MluCI AATT 5 cut(s) 5, 668, 844, 867, 1227
MlyI GAGTC 2 cut(s) 351, 757
MmeI TCCRAC 6 cut(s) 119, 370, 562, 703, 757, 1022
Mph1103I ATGCAT 1 cut(s) 415
MroXI GAANNNNTTC 2 cut(s) 459, 1227
MseI TTAA 6 cut(s) 44, 440, 890, 971, 1092, 1197
MslI CAYNNNNRTG 2 cut(s) 293, 486
MspA1I CMGCKG 1 cut(s) 1004
MspI CCGG 2 cut(s) 1148, 1204
MspR9I CCNGG 3 cut(s) 466, 924, 1205
Mva1269I GAATGC 1 cut(s) 413
MvaI CCWGG 2 cut(s) 466, 924
MvnI CGCG 2 cut(s) 1004, 1187
MwoI GCNNNNNNNGC 2 cut(s) 253, 371
NciI CCSGG 1 cut(s) 1205
NcoI CCATGG 1 cut(s) 288
NdeI CATATG 1 cut(s) 487
NdeII GATC 5 cut(s) 1021, 1054, 1129, 1311, 1333
NlaIII CATG 7 cut(s) 292, 298, 421, 454, 596, 1022, 1235
NlaIV GGNNCC 1 cut(s) 24
NmuCI GTSAC 1 cut(s) 878
NsiI ATGCAT 1 cut(s) 415
NspI RCATGY 1 cut(s) 596
PaeR7I CTCGAG 1 cut(s) 13
PagI TCATGA 2 cut(s) 417, 1018
PctI GAATGC 1 cut(s) 413
PdmI GAANNNNTTC 2 cut(s) 459, 1227
PfeI GAWTC 2 cut(s) 142, 1223
PflMI CCANNNNNTGG 1 cut(s) 791
PinAI ACCGGT 1 cut(s) 1147
PleI GAGTC 2 cut(s) 350, 757
PpsI GAGTC 2 cut(s) 350, 757
PpuMI RGGWCCY 1 cut(s) 1214
Psp1406I AACGTT 1 cut(s) 789
Psp5II RGGWCCY 1 cut(s) 1214
Psp6I CCWGG 2 cut(s) 464, 922
PspFI CCCAGC 1 cut(s) 1135
PspGI CCWGG 2 cut(s) 464, 922
PspN4I GGNNCC 1 cut(s) 24
PspPI GGNCC 1 cut(s) 1214
PspPPI RGGWCCY 1 cut(s) 1214
PspXI VCTCGAGB 1 cut(s) 13
PsrI GAACNNNNNNTAC 2 cut(s) 417, 449
PsuI RGATCY 1 cut(s) 1311
RsaI GTAC 3 cut(s) 984, 1202, 1290
RsaNI GTAC 3 cut(s) 983, 1201, 1289
RseI CAYNNNNRTG 2 cut(s) 293, 486
SacII CCGCGG 1 cut(s) 1005
SaqAI TTAA 6 cut(s) 44, 440, 890, 971, 1092, 1197
Sau3AI GATC 5 cut(s) 1021, 1054, 1129, 1311, 1333
Sau96I GGNCC 1 cut(s) 1214
ScaI AGTACT 1 cut(s) 1290
SchI GAGTC 2 cut(s) 351, 757
ScrFI CCNGG 3 cut(s) 466, 924, 1205
SduI GDGCHC 1 cut(s) 784
SfaNI GCATC 4 cut(s) 400, 490, 929, 1102
SfcI CTRYAG 1 cut(s) 714
Sfr274I CTCGAG 1 cut(s) 13
Sfr303I CCGCGG 1 cut(s) 1005
SgrAI CRCCGGYG 1 cut(s) 1147
SgrBI CCGCGG 1 cut(s) 1005
SinI GGWCC 1 cut(s) 1214
SlaI CTCGAG 1 cut(s) 13
SmiMI CAYNNNNRTG 2 cut(s) 293, 486
SmlI CTYRAG 2 cut(s) 13, 643
SmoI CTYRAG 2 cut(s) 13, 643
Sse9I AATT 5 cut(s) 5, 668, 844, 867, 1227
SsiI CCGC 3 cut(s) 1002, 1004, 1187
SspI AATATT 1 cut(s) 370
SspMI CTAG 3 cut(s) 177, 962, 1354
StyD4I CCNGG 3 cut(s) 464, 922, 1203
StyI CCWWGG 2 cut(s) 288, 609
TaaI ACNGT 1 cut(s) 819
TaiI ACGT 1 cut(s) 792
TaqI TCGA 1 cut(s) 14
TasI AATT 5 cut(s) 5, 668, 844, 867, 1227
TatI WGTACW 2 cut(s) 982, 1288
TfiI GAWTC 2 cut(s) 142, 1223
Tru1I TTAA 6 cut(s) 44, 440, 890, 971, 1092, 1197
Tru9I TTAA 6 cut(s) 44, 440, 890, 971, 1092, 1197
TscAI CASTG 2 cut(s) 258, 1183
TseFI GTSAC 1 cut(s) 878
Tsp45I GTSAC 1 cut(s) 878
TspRI CASTG 2 cut(s) 258, 1183
Van91I CCANNNNNTGG 1 cut(s) 791
VpaK11BI GGWCC 1 cut(s) 1214
XagI CCTNNNNNAGG 1 cut(s) 441
XapI RAATTY 1 cut(s) 867
XbaI TCTAGA 1 cut(s) 961
XceI RCATGY 1 cut(s) 596
XhoI CTCGAG 1 cut(s) 13
XmnI GAANNNNTTC 2 cut(s) 459, 1227
XspI CTAG 3 cut(s) 177, 962, 1354
ZrmI AGTACT 1 cut(s) 1290
Zsp2I ATGCAT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.