MD07G1087900.v1.1

Nucleolar GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005154
Physical Location & Seq
Forward (+)
9175883 .. 9182769
6887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1087900.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGAGCAGGGCTTCTACTCTCTTCCAATTGTGGCAGACCCCAAACAGATTGCCGCTCGCAATCTCTAAGATTTCAAAAGGTCCGCACTTGATACCGAGTGCCAGAAGTTATCCAGTACTGTGCTTCTGCAAGGAAATACAAACTAGCACATATGAGGTTGTCAAGGGAAGCTATGTACCTTCACCTGTTGTAAAACCAGTGAGCAAGGCCAAGGAAAAATCACCTGAAAATATTGAAGATGTTGGTGCATTCCAAAAGCTACCCATGGTGATGCCATCTGTTGACATTCTGTACTCAGCACTAAGGAAGGCTAAGAGGATAACACCAACGAAGGGTATTGCCAATATTGCAAAGCGAGAGAGAAATAGAGGTGCAAAGCAACTTGATGCATTGATGAAAGAACTGGCACTTCCGTTGAGAACTTATTCGGAGAATTTTCCAAATAAAAAGTATCTTCACCCTTACGAACGGTCTCTTATTGAATTAACTCTTGGGGATGGAAATTATGAACAGGTGTTGGGTAAGGTCGATGCTTTGAGGAAGAAGGTTGTGTCGATTGGAAAGGAACATGCATCTCTTTGTGCTAAGTCAACAACGAAGAAGGAAGCAGAGGCAAGACTAACTGAGGGTATAAAGAAAGTCGAAGAGACATTTAATCGTGAAGGAAAACATGTTGATGATTTGTTACACATAGCCAAGACTCTGCGAGCAATGCCAGTAGTTGATCTAGAAGCACCGACACTGTGCCTTGTTGGAGCTCCTAATGTCGGGAAGTCATCTTTGGTCCGTATACTCTCTACTGGGAAGCCTGAGATCTGCAACTACCCGTTCACAACAAGAGGAATACTAATGGGTCATATTATTTCAAGCCATCAGACTTTTCAGGTGACAGACACTCCTGGCCTGCTGAAGAGATGTGATGAGGACAGAAATAATTTGGAAAAATTGACACTTGCTGTCCTCTCACATTTACCGACCGCAATTCTCTATGTCCATGATCTCTCTGGGGAATGCGGGACCTCACCTTCTGACCAGTTTGTCATATACAAGGAAATAAAGGAGAGGTTTGGTGAGCATCTGTGGCTTGATGTTGTGTCCAAATGTGATCTGTTGCAAAAGTCTCCAGTGCTATTTGCCACCGATAATGGTGATGATTCTGATCTTAAGCTGGAAAGGTACCGGAAGTTGGGTCCCGATGGAGCTATCCATGTGTCAGTGATGAATGAAGTAGGGCTCGCCGAGTTGAAGAGTCGAGTGCAGGAGATGCTGGATTCTCAGATGACAAGGATCCAGGTTCAAGAAGGCAACAAACAAAAGCTTGAAGTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1 NOG1

Protein Analysis

444

Amino Acids

49.43

Weight (kDa)

9.04

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOG1_N PF17835 84 - 242 9.7e-51 NOG1 N-terminal helical domain
FeoB_N PF02421 247 - 304 1.8e-08 Ferrous iron transport protein B
MMR_HSR1 PF01926 248 - 344 2.1e-15 50S ribosome-binding GTPase
NOG1 PF06858 313 - 369 1.2e-17 Nucleolar GTP-binding protein 1 (NOG1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1037
Acc65I GGTACC 1 cut(s) 1176
AccB1I GGYRCC 1 cut(s) 1176
AccBSI CCGCTC 1 cut(s) 55
AccI GTMKAC 1 cut(s) 790
AciI CCGC 4 cut(s) 53, 83, 978, 1014
AclWI GGATC 2 cut(s) 1282, 1295
AcsI RAATTY 1 cut(s) 433
AcuI CTGAAG 1 cut(s) 929
AfaI GTAC 4 cut(s) 117, 177, 293, 1178
AfiI CCNNNNNNNGG 3 cut(s) 332, 767, 1186
AflII CTTAAG 1 cut(s) 1163
AflIII ACRYGT 1 cut(s) 670
AgsI TTSAA 7 cut(s) 75, 236, 482, 867, 1246, 1298, 1322
AjnI CCWGG 2 cut(s) 898, 1290
AjuI GAANNNNNNNTTGG 4 cut(s) 474, 506, 764, 796
AluBI AGCT 6 cut(s) 171, 259, 758, 1168, 1202, 1318
AluI AGCT 6 cut(s) 171, 259, 758, 1168, 1202, 1318
Alw21I GWGCWC 1 cut(s) 760
Alw26I GTCTC 3 cut(s) 477, 641, 1125
AlwI GGATC 2 cut(s) 1282, 1295
AoxI GGCC 2 cut(s) 207, 901
ApoI RAATTY 1 cut(s) 433
Asp700I GAANNNNTTC 1 cut(s) 424
Asp718I GGTACC 1 cut(s) 1176
AspS9I GGNCC 4 cut(s) 80, 784, 1017, 1190
AsuHPI GGTGA 8 cut(s) 174, 213, 280, 449, 898, 1014, 1082, 1160
AvaII GGWCC 4 cut(s) 80, 784, 1017, 1190
BamHI GGATCC 1 cut(s) 1287
BanI GGYRCC 1 cut(s) 1176
BanII GRGCYC 2 cut(s) 760, 1236
Bbv12I GWGCWC 1 cut(s) 760
BccI CCATC 4 cut(s) 283, 491, 879, 1190
BciT130I CCWGG 2 cut(s) 900, 1292
BcoDI GTCTC 3 cut(s) 477, 641, 1125
BfaI CTAG 2 cut(s) 144, 728
BfrI CTTAAG 1 cut(s) 1163
BglII AGATCT 1 cut(s) 813
BisI GCNGC 1 cut(s) 53
BlsI GCNGC 1 cut(s) 54
BmcAI AGTACT 1 cut(s) 117
Bme1390I CCNGG 2 cut(s) 900, 1292
Bme18I GGWCC 4 cut(s) 80, 784, 1017, 1190
BmgT120I GGNCC 4 cut(s) 80, 784, 1017, 1190
BmiI GGNNCC 5 cut(s) 1018, 1178, 1191, 1192, 1289
BmrFI CCNGG 2 cut(s) 900, 1292
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 6 cut(s) 261, 376, 520, 581, 1084, 1254
BmuI ACTGGG 1 cut(s) 810
BpmI CTGGAG 1 cut(s) 1107
BsaBI GATNNNNATC 1 cut(s) 1158
BsaI GGTCTC 1 cut(s) 477
BsaJI CCNNGG 2 cut(s) 210, 264
BsaWI WCCGGW 1 cut(s) 1179
BsaXI ACNNNNNCTCC 2 cut(s) 880, 910
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 767, 1186
Bse1I ACTGG 7 cut(s) 113, 197, 408, 716, 805, 1033, 1124
Bse3DI GCAATG 1 cut(s) 717
Bse8I GATNNNNATC 1 cut(s) 1158
BseBI CCWGG 2 cut(s) 900, 1292
BseDI CCNNGG 2 cut(s) 210, 264
BseGI GGATG 1 cut(s) 502
BseJI GATNNNNATC 1 cut(s) 1158
BseLI CCNNNNNNNGG 3 cut(s) 332, 767, 1186
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 4 cut(s) 309, 615, 801, 1289
BseNI ACTGG 7 cut(s) 113, 197, 408, 716, 805, 1033, 1124
BsgI GTGCAG 1 cut(s) 1277
Bsh1285I CGRYCG 1 cut(s) 978
BshFI GGCC 2 cut(s) 209, 903
BshNI GGYRCC 1 cut(s) 1176
BsiEI CGRYCG 1 cut(s) 978
BsiHKAI GWGCWC 1 cut(s) 760
BsiSI CCGG 1 cut(s) 1180
BslFI GGGAC 2 cut(s) 1030, 1176
BslI CCNNNNNNNGG 3 cut(s) 332, 767, 1186
BsmAI GTCTC 3 cut(s) 477, 641, 1125
BsmFI GGGAC 2 cut(s) 1030, 1176
BsmI GAATGC 2 cut(s) 248, 1016
BsnI GGCC 2 cut(s) 209, 903
Bso31I GGTCTC 1 cut(s) 477
Bsp1286I GDGCHC 2 cut(s) 760, 1236
Bsp143I GATC 6 cut(s) 724, 813, 997, 1105, 1159, 1287
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 4 cut(s) 53, 83, 978, 1014
BspANI GGCC 2 cut(s) 209, 903
BspCNI CTCAG 4 cut(s) 308, 616, 802, 1288
BspLI GGNNCC 5 cut(s) 1018, 1178, 1191, 1192, 1289
BspPI GGATC 2 cut(s) 1282, 1295
BspT107I GGYRCC 1 cut(s) 1176
BspTI CTTAAG 1 cut(s) 1163
BspTNI GGTCTC 1 cut(s) 477
BsrBI CCGCTC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 7 cut(s) 113, 197, 408, 716, 805, 1033, 1124
BssECI CCNNGG 2 cut(s) 210, 264
BssMI GATC 6 cut(s) 724, 813, 997, 1105, 1159, 1287
BssNAI GTATAC 1 cut(s) 791
BssT1I CCWWGG 2 cut(s) 210, 264
Bst1107I GTATAC 1 cut(s) 791
Bst2UI CCWGG 2 cut(s) 900, 1292
Bst4CI ACNGT 3 cut(s) 120, 471, 744
Bst6I CTCTTC 4 cut(s) 26, 639, 905, 1241
BstAFI CTTAAG 1 cut(s) 1163
BstAPI GCANNNNNTGC 1 cut(s) 1264
BstC8I GCNNGC 4 cut(s) 57, 708, 905, 1236
BstDEI CTNAG 8 cut(s) 66, 295, 302, 312, 585, 624, 810, 1275
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 1 cut(s) 502
BstKTI GATC 6 cut(s) 727, 816, 1000, 1108, 1162, 1290
BstMAI GTCTC 3 cut(s) 477, 641, 1125
BstMBI GATC 6 cut(s) 724, 813, 997, 1105, 1159, 1287
BstMCI CGRYCG 1 cut(s) 978
BstMWI GCNNNNNNNGC 4 cut(s) 347, 712, 1081, 1264
BstNI CCWGG 2 cut(s) 900, 1292
BstNSI RCATGY 2 cut(s) 572, 674
BstSCI CCNGG 2 cut(s) 898, 1290
BstX2I RGATCY 2 cut(s) 813, 1287
BstYI RGATCY 2 cut(s) 813, 1287
BstZ17I GTATAC 1 cut(s) 791
BsuRI GGCC 2 cut(s) 209, 903
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 1 cut(s) 502
BtsIMutI CAGTG 4 cut(s) 204, 740, 1131, 1221
Cac8I GCNNGC 4 cut(s) 57, 708, 905, 1236
Cfr13I GGNCC 4 cut(s) 80, 784, 1017, 1190
Csp6I GTAC 4 cut(s) 116, 176, 292, 1177
CviAII CATG 5 cut(s) 265, 569, 671, 995, 1208
CviQI GTAC 4 cut(s) 116, 176, 292, 1177
DdeI CTNAG 8 cut(s) 66, 295, 302, 312, 585, 624, 810, 1275
DpnI GATC 6 cut(s) 726, 815, 999, 1107, 1161, 1289
DpnII GATC 6 cut(s) 724, 813, 997, 1105, 1159, 1287
DrdI GACNNNNNNGTC 1 cut(s) 1037
DseDI GACNNNNNNGTC 1 cut(s) 1037
Eam1104I CTCTTC 4 cut(s) 26, 639, 905, 1241
EarI CTCTTC 4 cut(s) 26, 639, 905, 1241
Ecl136II GAGCTC 1 cut(s) 758
Eco130I CCWWGG 2 cut(s) 210, 264
Eco24I GRGCYC 2 cut(s) 760, 1236
Eco31I GGTCTC 1 cut(s) 477
Eco47I GGWCC 4 cut(s) 80, 784, 1017, 1190
Eco53kI GAGCTC 1 cut(s) 758
Eco57I CTGAAG 1 cut(s) 929
EcoICRI GAGCTC 1 cut(s) 758
EcoO109I RGGNCCY 2 cut(s) 1017, 1190
EcoRII CCWGG 2 cut(s) 898, 1290
EcoT14I CCWWGG 2 cut(s) 210, 264
EcoT22I ATGCAT 2 cut(s) 391, 574
EcoT38I GRGCYC 2 cut(s) 760, 1236
ErhI CCWWGG 2 cut(s) 210, 264
FaeI CATG 5 cut(s) 268, 572, 674, 998, 1211
FaqI GGGAC 2 cut(s) 1030, 1176
FatI CATG 5 cut(s) 264, 568, 670, 994, 1207
FauI CCCGC 1 cut(s) 1007
FauNDI CATATG 1 cut(s) 151
FblI GTMKAC 1 cut(s) 790
Fnu4HI GCNGC 1 cut(s) 53
FokI GGATG 1 cut(s) 509
FriOI GRGCYC 2 cut(s) 760, 1236
Fsp4HI GCNGC 1 cut(s) 53
FspBI CTAG 2 cut(s) 144, 728
GluI GCNGC 1 cut(s) 53
GsuI CTGGAG 1 cut(s) 1107
HaeIII GGCC 2 cut(s) 209, 903
HapII CCGG 1 cut(s) 1180
Hin1II CATG 5 cut(s) 268, 572, 674, 998, 1211
HincII GTYRAC 2 cut(s) 283, 591
HindII GTYRAC 2 cut(s) 283, 591
HindIII AAGCTT 1 cut(s) 1316
HinfI GANTC 4 cut(s) 700, 1154, 1249, 1271
HpaII CCGG 1 cut(s) 1180
HphI GGTGA 8 cut(s) 174, 213, 280, 449, 898, 1014, 1082, 1160
Hpy166II GTNNAC 4 cut(s) 283, 591, 791, 831
Hpy188I TCNGA 5 cut(s) 430, 876, 1030, 1159, 1278
Hpy188III TCNNGA 5 cut(s) 659, 728, 769, 1193, 1298
Hpy8I GTNNAC 4 cut(s) 283, 591, 791, 831
HpyAV CCTTC 8 cut(s) 189, 301, 325, 538, 595, 656, 1035, 1295
HpyCH4III ACNGT 3 cut(s) 120, 471, 744
HpyCH4V TGCA 9 cut(s) 129, 248, 350, 374, 389, 572, 819, 1114, 1258
HpyF10VI GCNNNNNNNGC 4 cut(s) 347, 712, 1081, 1264
HpyF3I CTNAG 8 cut(s) 66, 295, 302, 312, 585, 624, 810, 1275
Hsp92II CATG 5 cut(s) 268, 572, 674, 998, 1211
KflI GGGWCCC 1 cut(s) 1190
KpnI GGTACC 1 cut(s) 1180
Kzo9I GATC 6 cut(s) 724, 813, 997, 1105, 1159, 1287
LmnI GCTCC 3 cut(s) 755, 763, 1199
LweI GCATC 6 cut(s) 261, 376, 520, 581, 1084, 1254
MaeI CTAG 2 cut(s) 144, 728
MaeIII GTNAC 2 cut(s) 684, 886
MalI GATC 6 cut(s) 726, 815, 999, 1107, 1161, 1289
MbiI CCGCTC 1 cut(s) 55
MboI GATC 6 cut(s) 724, 813, 997, 1105, 1159, 1287
MboII GAAGA 8 cut(s) 13, 248, 446, 553, 610, 656, 922, 1258
MfeI CAATTG 1 cut(s) 26
MflI RGATCY 2 cut(s) 813, 1287
MhlI GDGCHC 2 cut(s) 760, 1236
MluCI AATT 7 cut(s) 26, 433, 482, 502, 934, 944, 981
MlyI GAGTC 2 cut(s) 694, 1258
MmeI TCCRAC 1 cut(s) 733
Mph1103I ATGCAT 2 cut(s) 391, 574
MroXI GAANNNNTTC 1 cut(s) 424
MseI TTAA 4 cut(s) 485, 654, 1164, 1330
MslI CAYNNNNRTG 2 cut(s) 269, 675
MspCI CTTAAG 1 cut(s) 1163
MspI CCGG 1 cut(s) 1180
MspR9I CCNGG 2 cut(s) 900, 1292
MunI CAATTG 1 cut(s) 26
Mva1269I GAATGC 2 cut(s) 248, 1016
MvaI CCWGG 2 cut(s) 900, 1292
MwoI GCNNNNNNNGC 4 cut(s) 347, 712, 1081, 1264
NcoI CCATGG 1 cut(s) 264
NdeI CATATG 1 cut(s) 151
NdeII GATC 6 cut(s) 724, 813, 997, 1105, 1159, 1287
NlaIII CATG 5 cut(s) 268, 572, 674, 998, 1211
NlaIV GGNNCC 5 cut(s) 1018, 1178, 1191, 1192, 1289
NmeAIII GCCGAG 1 cut(s) 1264
NmuCI GTSAC 1 cut(s) 886
NsiI ATGCAT 2 cut(s) 391, 574
NspI RCATGY 2 cut(s) 572, 674
PciI ACATGT 1 cut(s) 670
PctI GAATGC 2 cut(s) 248, 1016
PdmI GAANNNNTTC 1 cut(s) 424
PfeI GAWTC 2 cut(s) 1154, 1271
PkrI GCNGC 1 cut(s) 54
PleI GAGTC 2 cut(s) 694, 1257
PpsI GAGTC 2 cut(s) 694, 1257
PpuMI RGGWCCY 2 cut(s) 1017, 1190
PscI ACATGT 1 cut(s) 670
Psp124BI GAGCTC 1 cut(s) 760
Psp5II RGGWCCY 2 cut(s) 1017, 1190
Psp6I CCWGG 2 cut(s) 898, 1290
PspGI CCWGG 2 cut(s) 898, 1290
PspN4I GGNNCC 5 cut(s) 1018, 1178, 1191, 1192, 1289
PspPI GGNCC 4 cut(s) 80, 784, 1017, 1190
PspPPI RGGWCCY 2 cut(s) 1017, 1190
PsuI RGATCY 2 cut(s) 813, 1287
RsaI GTAC 4 cut(s) 117, 177, 293, 1178
RsaNI GTAC 4 cut(s) 116, 176, 292, 1177
RseI CAYNNNNRTG 2 cut(s) 269, 675
SacI GAGCTC 1 cut(s) 760
SaqAI TTAA 4 cut(s) 485, 654, 1164, 1330
SatI GCNGC 1 cut(s) 53
Sau3AI GATC 6 cut(s) 724, 813, 997, 1105, 1159, 1287
Sau96I GGNCC 4 cut(s) 80, 784, 1017, 1190
ScaI AGTACT 1 cut(s) 117
SchI GAGTC 2 cut(s) 694, 1258
ScrFI CCNGG 2 cut(s) 900, 1292
SduI GDGCHC 2 cut(s) 760, 1236
SfaNI GCATC 6 cut(s) 261, 376, 520, 581, 1084, 1254
SinI GGWCC 4 cut(s) 80, 784, 1017, 1190
SmiMI CAYNNNNRTG 2 cut(s) 269, 675
SmlI CTYRAG 1 cut(s) 1163
SmoI CTYRAG 1 cut(s) 1163
Sse9I AATT 7 cut(s) 26, 433, 482, 502, 934, 944, 981
SsiI CCGC 4 cut(s) 53, 83, 978, 1014
SspI AATATT 2 cut(s) 232, 346
SspMI CTAG 2 cut(s) 144, 728
SstI GAGCTC 1 cut(s) 760
StyD4I CCNGG 2 cut(s) 898, 1290
StyI CCWWGG 2 cut(s) 210, 264
TaaI ACNGT 3 cut(s) 120, 471, 744
TaqI TCGA 4 cut(s) 528, 554, 642, 1252
TasI AATT 7 cut(s) 26, 433, 482, 502, 934, 944, 981
TatI WGTACW 2 cut(s) 115, 291
TauI GCSGC 1 cut(s) 55
TfiI GAWTC 2 cut(s) 1154, 1271
Tru1I TTAA 4 cut(s) 485, 654, 1164, 1330
Tru9I TTAA 4 cut(s) 485, 654, 1164, 1330
TscAI CASTG 4 cut(s) 204, 747, 1131, 1221
TseFI GTSAC 1 cut(s) 886
Tsp45I GTSAC 1 cut(s) 886
TspDTI ATGAA 4 cut(s) 410, 522, 1235, 1239
TspGWI ACGGA 2 cut(s) 402, 776
TspRI CASTG 4 cut(s) 204, 747, 1131, 1221
Vha464I CTTAAG 1 cut(s) 1163
VpaK11BI GGWCC 4 cut(s) 80, 784, 1017, 1190
XapI RAATTY 1 cut(s) 433
XbaI TCTAGA 1 cut(s) 727
XceI RCATGY 2 cut(s) 572, 674
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XmiI GTMKAC 1 cut(s) 790
XmnI GAANNNNTTC 1 cut(s) 424
XspI CTAG 2 cut(s) 144, 728
ZrmI AGTACT 1 cut(s) 117
Zsp2I ATGCAT 2 cut(s) 391, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.