Rorug01G0169200

Nucleolar GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005154
Physical Location & Seq
Reverse (-)
23062496 .. 23066896
4401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0169200.1

Sequence Viewer

Length: 3657 bp
ATGGCTCAGTTCCGAGGTCACAAGGCCTCCCATTCCAGATCTACTTCTTCTCCTTCTGGACTAGGTTATGACGTGTTCCTGAGCTTCAGAGGTGAAGACACCCGAAAGAACTTCACTGATCACCTCCACACGGCCCTTCTCAACTCCCGATTTCGAACTTTTCGGGATGACCCTGAACTCAAAAGAGGGGAGAATATCAAGGAAGAGCTTGAGAAAGCCATTCAACAGTCACGAAGTTCTGTGATTGTGTTTTCGAAAGACTACGCCTCTTCCAAATGGTGCCTCGACGAGCTTGTCATGATCCTTGAACGCAAGAGGACCTCCAATCATTTCGTTTTACCAGTCTTCTACGATGTTGATCCATCCCATCTGAGGAAGCAGACTGTAAGTGTTGCAAAAGCATTTACTGGACACCAGAAAACTCAATCGTTGAGTAAAGTGAGAAGATGGAGGGCAGCACTTAGAGAAGTTGCAGATCTAGCAGGAATGAATTTACAAAATGAAGCTGACGGGCACGAGTCAAAGTTCATCCAAAAGATTGTTACAGTGATTCAAGAAAAGCTAGGTCCTGTTATTGTGCCCCTAAGTGATGTCCAGAAGGAATTAGAGGAAAAGTTGTTATCTGTTAACCCTGTTCTTGATGACGCCGAGGGGAAGCAACTGAAGTTCCAAATGACTGTGAAGCCATGGCTCCATAAGCTTAAAGAGGCTGTCTATGAGGCTGAGGACCTCTTGCAGGAGATCAAAACTGAAGCATTGAGGCAAAAGATAGAACACAAATATAGGAGTAGCACCAGTAAGGTACAAGAGCCCATCTCTACTCCATCTCATGCCTTTGACTCAGCTTTAATATGCTCCAGGGTAGAAGAAGTTCTTAAGAGACTAGACAACATTTCAAAGCAGACAAAAGATGTCCTGGATTCAAAAATAACCGCTGGACACAGAGTATCACAAACACTATCGTCAACTTTTTTGGTAGAAAAGTCTGTATATGGAAGAGATGAAGAGAAAGAAAGATTGATTAAATTATTGCTATCAGATGATGAGACTGGCAATGAAATAGGTGTGATTCCTATTGTGGGTATGGGCGGAATCGGAAAGACCACCCTTGCCCAATTTGTATACAACGATGCTAGAGTGAAGCAACATTTTGACCTCCAAGCATGGGTTTGCGTTTCAAAAGAATTTGATGTTTTTAGGATCTTGCAACACATTTATGAGTCCCTCACCAAAAAAGCTTGCAAGATCACGAACCTTGATCTGCTTCTATCAAAGTTGCAGGAAATTTTGACGGGGAAAAGGTTCTTCTTTGTTCTTGATGACATCTGGAACAAGAATTATAACCAGTTGGAATTCTTAAGGCGTCCCCTTGAGTCTGGAGCACATGGAAGCAGGATCATTGTCACAACACGTGATGAAAATGTTGCACGTATGATGGGTAGCCTTAAAGCTCATCGTCTCATGCCAATGTCTGAGGAAGATAGCTGGTCTTTATTCAAAAAACATGCCTTGAAAAATGCAGGTGTTGGTACACACTCACATCTTGAAAAAATTGGTAGACAAATTGTTCGAAAGTGCAATGGTCTTCCTTTGGCTGTTAAGTCCCTTGGGGGTCTCTTATGTTCTAAATTGTTAGTGGAGGAATGGGAAAGTATATTGAACAGTGACATGTGGGAGTTTTCACCGGATGAGAGTGACATTCTGCCATCTCTATGGTTGAGTTATCTAGATCTGCCTCTACATCTCAAGCGTTGTTTTGCCTATTGTTCACTATATCCCAAGAACTATGAATTTCAAAAATCAGAGTTGGTTTACTTGTGGAAGGCTGAAGATCTATTGCAACATAAAAAAAACAAAATGGTAGAAGAAGTTGGACAAGACTACTTCAATGATCTAATCTCAAGATCATTTTTTCAACTTTCATCAAGTTCCGATCAATATTTCATGCATGACCTTATCCATGATTTAGCAAGCTTTGTATCTGGAGAATTTTGTTTTAGGTGTGAGGGCAGTGATTCACCCAACAGTTTGAGCAAGACTCGTTATTTTTCAGATTATCATGATGAAGCTGATAGCAGTACCGTAGAGATGTTTGAGGCTTTACAGCAAGCTAAATGTTTACGCACCTTTCTATCATTAAAATCATGTCGGTGGTGGATGTTAAAAGCACGATGTGGTATATTGCCAAAATCAAAATGTTTAAGAGTGCTCAAGTTATACGGGTACAATATTAAGGAATTGCCTGATTCAATTAACAACTTCAAACATCTTAGGTACTTGGACTTGTCTTGGACTCCCATCGAAAAGTTACCTGATACAATTTGTACGTTGTATAACTTGCAAGTATTGTTGTTGTCAAACTGTGACTACCTTACTGAACTGCCAGCCAACTTGGGAAGATTGATCAACTTGAGCCATCTGGATACTACAGGTATAAAGTTAAAAAAGATGCCACCTGATATGGGTAACTTGAAAGACCTCCAGATGCTACCCGAGTTTGTGCTAGACAAAAATACTGCAGGGGATAGTTTGGCAGTGTTAAAGAAGCTTGAAAAATTGCGTGGAAGACTTCGTATCTCAGGGCTTGTGCATAGCAGCGGTTTGGAGGCCTACATACTGAGGGACAAGAAGTTTCTTAAGGAACTGGTTTTGGATTGGAGAGGAAGGGATTCAAGTGAAGAAGGCAATAATACTGTAGAACAAAGAGAAGTGCTTGAGAAGCTCCAACCTCACCTGAACCTGGAAAAGCTTAAAATTGAAGGTTATGGAGGCAAAATGTTTCCAGGTTGGTCAGAATATTATTTTTCTTCTGCTCTTGTTTATCTTAAACTTGTTAATTGTGAGAATTGTATCTCCTTGCCACCATTGGGACAGCTACCTTCCCTCACAGAGCTTGATATTTCTGGGTTACATGGAGTGGTGTCCATAGGTTCCGAATTCTATTGTAGTGACGGCAATACTACTACTTGTGTGAATAAACCATTTAGATCTCTACGGGCTTTAAAATTCTACAATATGAGAGGGTGGCAAGAATGGTCTTATGTTGGAGGTGAAAATAAAGAAGGTGGGGTTTTTCCTAATCTTGTTCAACTTGAAGTATTATACTGTCCCAATCTTACCGGAAGATTAGCGTCAGACAGTCTCCCATCTCTTGTGTATTTGTTGGTATGGAATTGCCCAGAATTCCAATGTATTCCGGAAGGTGGATTCGCATCAAAGTTAAAATCACTTGACATCAGCGGCTGTAAAAAAGTGAAAGCAAAGAGTAAGCAGAATCTGAACAAGGGTCTCCGGGCACTCACCTCTCTTGAAAGATTGTCACTTGATTTTGACTGCGACGAAAATGTAGATTGGTTTGCGGAGGAGCAGGAGGAGCTGTTTCCCAGCACTCTTACATATCTCCAGATCTGCCATCTGAATTGTGAGACCATCCACGGCGCCAAGTGGTTTGGCCATCTCAACTCTCTTCAAACATTGATTATATTTAATTGCCTTGCGCTCCGGTGCCTGCCAGATAGTGGACTGCCCACTTCTCTTTCTCATCTAAATATTCATGGATGCCCTTTGCTTGAGAAACGGTGCCAAAGAGAGACGGGTGAAGATTGGCCCAAGATTGCTCACATCAAGCGCATAGTTATCAATTTGGATATGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1 NOG1

Protein Analysis

1218

Amino Acids

139.19

Weight (kDa)

7.81

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 190 1.2e-48 TIR domain
TIR_2 PF13676 25 - 129 7e-17 TIR domain
Rx_N PF18052 176 - 262 2.8e-13 Rx N-terminal domain
NB-ARC PF00931 334 - 506 1.3e-40 NB-ARC domain
WHD_DRP PF23559 591 - 657 3.2e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 731 - 1007 5e-26 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 845 - 969 1.2e-30 R13L1/DRL21 LRRs
LRR_R13L1-DRL21 PF25019 1075 - 1187 1.6e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1341
AarI CACCTGC 1 cut(s) 1511
AasI GACNNNNNNGTC 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 1511
AccB1I GGYRCC 4 cut(s) 279, 3437, 3504, 3579
AccB7I CCANNNNNTGG 1 cut(s) 3518
AccI GTMKAC 2 cut(s) 1122, 1558
AccIII TCCGGA 1 cut(s) 3196
AciI CCGC 5 cut(s) 933, 1089, 2598, 3240, 3359
AclWI GGATC 4 cut(s) 295, 353, 1208, 1403
AcoI YGGCCR 1 cut(s) 3451
AcsI RAATTY 9 cut(s) 490, 1184, 1284, 1352, 1790, 1988, 2936, 3005, 3182
AcuI CTGAAG 4 cut(s) 70, 683, 771, 1848
AcvI CACGTG 1 cut(s) 1412
AcyI GRCGYC 3 cut(s) 645, 1363, 3438
AdeI CACNNNGTG 1 cut(s) 2174
AfaI GTAC 6 cut(s) 804, 1531, 2080, 2225, 2276, 2326
AflII CTTAAG 3 cut(s) 875, 1357, 2636
AflIII ACRYGT 3 cut(s) 72, 1409, 1668
AjiI CACGTC 1 cut(s) 73
AjnI CCWGG 4 cut(s) 857, 915, 2739, 2782
Alw21I GWGCWC 2 cut(s) 1384, 2211
Alw26I GTCTC 8 cut(s) 874, 1040, 1463, 1619, 3146, 3293, 3419, 3584
AlwI GGATC 4 cut(s) 295, 353, 1208, 1403
AlwNI CAGNNNCTG 2 cut(s) 3243, 3277
Ama87I CYCGRG 1 cut(s) 2492
Aor13HI TCCGGA 1 cut(s) 3196
AoxI GGCC 5 cut(s) 24, 132, 2607, 3451, 3605
ApeKI GCWGC 2 cut(s) 455, 2595
ApoI RAATTY 9 cut(s) 490, 1184, 1284, 1352, 1790, 1988, 2936, 3005, 3182
ArsI GACNNNNNNTTYG 2 cut(s) 1551, 1583
Asp700I GAANNNNTTC 3 cut(s) 870, 1301, 2668
AspLEI GCGC 3 cut(s) 3440, 3499, 3630
AspS9I GGNCC 5 cut(s) 133, 318, 566, 727, 3606
AsuC2I CCSGG 1 cut(s) 3293
AsuHPI GGTGA 9 cut(s) 104, 113, 1219, 1674, 2010, 2723, 3062, 3292, 3608
AsuII TTCGAA 3 cut(s) 154, 254, 1570
AvaI CYCGRG 1 cut(s) 2492
AvaII GGWCC 3 cut(s) 318, 566, 727
BaeGI GKGCMC 3 cut(s) 516, 582, 3298
BalI TGGCCA 1 cut(s) 3453
BanI GGYRCC 4 cut(s) 279, 3437, 3504, 3579
BanII GRGCYC 1 cut(s) 813
BauI CACGAG 1 cut(s) 515
BbrPI CACGTG 1 cut(s) 1412
BbsI GAAGAC 4 cut(s) 102, 337, 1577, 2572
Bbv12I GWGCWC 2 cut(s) 1384, 2211
BbvCI CCTCAGC 1 cut(s) 723
BbvI GCAGC 2 cut(s) 467, 2607
BceAI ACGGC 3 cut(s) 147, 2968, 3451
BciT130I CCWGG 4 cut(s) 859, 917, 2741, 2784
BciVI GTATCC 1 cut(s) 2416
BclI TGATCA 2 cut(s) 118, 2403
BcnI CCSGG 1 cut(s) 3293
BcoDI GTCTC 8 cut(s) 874, 1040, 1463, 1619, 3146, 3293, 3419, 3584
BfaI CTAG 7 cut(s) 62, 479, 563, 884, 1134, 1727, 2504
BfmI CTRYAG 3 cut(s) 2427, 2517, 2694
BfoI RGCGCY 1 cut(s) 3441
BfrI CTTAAG 3 cut(s) 875, 1357, 2636
BfuAI ACCTGC 1 cut(s) 1511
BfuI GTATCC 1 cut(s) 2416
BglII AGATCT 6 cut(s) 38, 475, 1729, 1831, 2987, 3405
BisI GCNGC 3 cut(s) 456, 2596, 3241
BlsI GCNGC 3 cut(s) 457, 2597, 3242
Bme1390I CCNGG 5 cut(s) 859, 917, 2741, 2784, 3293
Bme18I GGWCC 3 cut(s) 318, 566, 727
BmeT110I CYCGRG 1 cut(s) 2492
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 5 cut(s) 133, 318, 566, 727, 3606
BmiI GGNNCC 6 cut(s) 281, 692, 2932, 3439, 3506, 3581
BmrFI CCNGG 5 cut(s) 859, 917, 2741, 2784, 3293
BmsI GCATC 5 cut(s) 1120, 2439, 2475, 3221, 3548
BpiI GAAGAC 4 cut(s) 102, 337, 1577, 2572
BplI GAGNNNNNCTC 4 cut(s) 800, 832, 2023, 2055
BpmI CTGGAG 5 cut(s) 841, 1398, 2004, 2465, 3386
Bpu10I CCTNAGC 2 cut(s) 80, 723
Bpu14I TTCGAA 3 cut(s) 154, 254, 1570
BpuEI CTTGAG 8 cut(s) 230, 1391, 1730, 1885, 2195, 2431, 2735, 3590
BpuMI CCSGG 1 cut(s) 3293
BsaAI YACGTR 2 cut(s) 1412, 1430
BsaBI GATNNNNATC 2 cut(s) 357, 3211
BsaHI GRCGYC 3 cut(s) 645, 1363, 3438
BsaI GGTCTC 3 cut(s) 1619, 3293, 3419
BsaJI CCNNGG 6 cut(s) 13, 648, 686, 858, 1606, 3433
BsaWI WCCGGW 4 cut(s) 1684, 3119, 3196, 3501
BsaXI ACNNNNNCTCC 6 cut(s) 11, 34, 41, 64, 3362, 3392
Bse1I ACTGG 6 cut(s) 341, 412, 795, 1054, 1345, 2649
Bse3DI GCAATG 2 cut(s) 1060, 1585
Bse8I GATNNNNATC 2 cut(s) 357, 3211
BseAI TCCGGA 1 cut(s) 3196
BseBI CCWGG 4 cut(s) 859, 917, 2741, 2784
BseDI CCNNGG 6 cut(s) 13, 648, 686, 858, 1606, 3433
BseGI GGATG 7 cut(s) 172, 362, 528, 1693, 2163, 3429, 3563
BseJI GATNNNNATC 2 cut(s) 357, 3211
BseMI GCAATG 2 cut(s) 1060, 1585
BseMII CTCAG 8 cut(s) 20, 71, 362, 714, 855, 1464, 2592, 2609
BseNI ACTGG 6 cut(s) 341, 412, 795, 1054, 1345, 2649
BseRI GAGGAG 2 cut(s) 3377, 3386
BseSI GKGCMC 3 cut(s) 516, 582, 3298
BseXI GCAGC 2 cut(s) 467, 2607
BseYI CCCAGC 1 cut(s) 3383
BshFI GGCC 5 cut(s) 26, 134, 2609, 3453, 3607
BshNI GGYRCC 4 cut(s) 279, 3437, 3504, 3579
BsiHKAI GWGCWC 2 cut(s) 1384, 2211
BsiHKCI CYCGRG 1 cut(s) 2492
BsiSI CCGG 5 cut(s) 1685, 3120, 3197, 3292, 3502
BslFI GGGAC 6 cut(s) 1207, 1350, 1588, 2636, 2883, 3093
BsmAI GTCTC 8 cut(s) 874, 1040, 1463, 1619, 3146, 3293, 3419, 3584
BsmBI CGTCTC 2 cut(s) 1463, 3584
BsmFI GGGAC 6 cut(s) 1207, 1350, 1588, 2636, 2883, 3093
BsnI GGCC 5 cut(s) 26, 134, 2609, 3453, 3607
Bso31I GGTCTC 3 cut(s) 1619, 3293, 3419
BsoBI CYCGRG 1 cut(s) 2492
Bsp119I TTCGAA 3 cut(s) 154, 254, 1570
Bsp1286I GDGCHC 6 cut(s) 516, 582, 813, 1384, 2211, 3298
Bsp13I TCCGGA 1 cut(s) 3196
Bsp19I CCATGG 1 cut(s) 686
BspACI CCGC 5 cut(s) 933, 1089, 2598, 3240, 3359
BspANI GGCC 5 cut(s) 26, 134, 2609, 3453, 3607
BspCNI CTCAG 8 cut(s) 19, 72, 363, 715, 854, 1465, 2591, 2610
BspEI TCCGGA 1 cut(s) 3196
BspHI TCATGA 2 cut(s) 297, 2059
BspLI GGNNCC 6 cut(s) 281, 692, 2932, 3439, 3506, 3581
BspMAI CTGCAG 1 cut(s) 2521
BspMI ACCTGC 1 cut(s) 1511
BspPI GGATC 4 cut(s) 295, 353, 1208, 1403
BspQI GCTCTTC 1 cut(s) 198
BspT104I TTCGAA 3 cut(s) 154, 254, 1570
BspT107I GGYRCC 4 cut(s) 279, 3437, 3504, 3579
BspTI CTTAAG 3 cut(s) 875, 1357, 2636
BspTNI GGTCTC 3 cut(s) 1619, 3293, 3419
BsrDI GCAATG 2 cut(s) 1060, 1585
BsrI ACTGG 6 cut(s) 341, 412, 795, 1054, 1345, 2649
BssECI CCNNGG 6 cut(s) 13, 648, 686, 858, 1606, 3433
BssNAI GTATAC 1 cut(s) 1123
BssNI GRCGYC 3 cut(s) 645, 1363, 3438
BssSI CACGAG 1 cut(s) 515
BssT1I CCWWGG 2 cut(s) 686, 1606
Bst1107I GTATAC 1 cut(s) 1123
Bst2BI CACGAG 1 cut(s) 515
Bst2UI CCWGG 4 cut(s) 859, 917, 2741, 2784
Bst6I CTCTTC 5 cut(s) 198, 274, 991, 999, 3471
BstACI GRCGYC 3 cut(s) 645, 1363, 3438
BstAFI CTTAAG 3 cut(s) 875, 1357, 2636
BstBAI YACGTR 2 cut(s) 1412, 1430
BstBI TTCGAA 3 cut(s) 154, 254, 1570
BstC8I GCNNGC 5 cut(s) 1240, 1972, 2109, 2385, 3509
BstDSI CCRYGG 2 cut(s) 686, 3433
BstENI CCTNNNNNAGG 1 cut(s) 734
BstF5I GGATG 7 cut(s) 172, 362, 528, 1693, 2163, 3429, 3563
BstH2I RGCGCY 1 cut(s) 3441
BstHHI GCGC 3 cut(s) 3440, 3499, 3630
BstMAI GTCTC 8 cut(s) 874, 1040, 1463, 1619, 3146, 3293, 3419, 3584
BstMWI GCNNNNNNNGC 4 cut(s) 479, 697, 2719, 3373
BstNI CCWGG 4 cut(s) 859, 917, 2741, 2784
BstNSI RCATGY 2 cut(s) 1508, 1672
BstSCI CCNGG 5 cut(s) 857, 915, 2739, 2782, 3291
BstSFI CTRYAG 3 cut(s) 2427, 2517, 2694
BstSLI GKGCMC 3 cut(s) 516, 582, 3298
BstV1I GCAGC 2 cut(s) 467, 2607
BstV2I GAAGAC 4 cut(s) 102, 337, 1577, 2572
BstX2I RGATCY 7 cut(s) 38, 475, 1200, 1729, 1831, 2987, 3405
BstXI CCANNNNNNTGG 1 cut(s) 1713
BstYI RGATCY 7 cut(s) 38, 475, 1200, 1729, 1831, 2987, 3405
BstZ17I GTATAC 1 cut(s) 1123
BsuI GTATCC 1 cut(s) 2416
BsuRI GGCC 5 cut(s) 26, 134, 2609, 3453, 3607
BtgI CCRYGG 2 cut(s) 686, 3433
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 7 cut(s) 172, 362, 528, 1693, 2163, 3429, 3563
BtsI GCAGTG 2 cut(s) 2017, 2541
BtsIMutI CAGTG 5 cut(s) 114, 552, 1669, 2017, 2541
BveI ACCTGC 1 cut(s) 1511
Cac8I GCNNGC 5 cut(s) 1240, 1972, 2109, 2385, 3509
CaiI CAGNNNCTG 2 cut(s) 3243, 3277
CciI TCATGA 2 cut(s) 297, 2059
CfoI GCGC 3 cut(s) 3440, 3499, 3630
Cfr13I GGNCC 5 cut(s) 133, 318, 566, 727, 3606
CseI GACGC 3 cut(s) 653, 1352, 3120
Csp6I GTAC 6 cut(s) 803, 1530, 2079, 2224, 2275, 2325
CviQI GTAC 6 cut(s) 803, 1530, 2079, 2224, 2275, 2325
DinI GGCGCC 1 cut(s) 3439
DraI TTTAAA 1 cut(s) 3003
DraIII CACNNNGTG 1 cut(s) 2174
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
EaeI YGGCCR 1 cut(s) 3451
Eam1104I CTCTTC 5 cut(s) 198, 274, 991, 999, 3471
EarI CTCTTC 5 cut(s) 198, 274, 991, 999, 3471
EciI GGCGGA 1 cut(s) 1104
Eco130I CCWWGG 2 cut(s) 686, 1606
Eco147I AGGCCT 2 cut(s) 26, 2609
Eco24I GRGCYC 1 cut(s) 813
Eco31I GGTCTC 3 cut(s) 1619, 3293, 3419
Eco47I GGWCC 3 cut(s) 318, 566, 727
Eco57I CTGAAG 4 cut(s) 70, 683, 771, 1848
Eco72I CACGTG 1 cut(s) 1412
Eco88I CYCGRG 1 cut(s) 2492
EcoNI CCTNNNNNAGG 1 cut(s) 734
EcoO109I RGGNCCY 3 cut(s) 318, 566, 727
EcoRI GAATTC 3 cut(s) 1352, 2936, 3182
EcoRII CCWGG 4 cut(s) 857, 915, 2739, 2782
EcoT14I CCWWGG 2 cut(s) 686, 1606
EcoT22I ATGCAT 1 cut(s) 1950
EcoT38I GRGCYC 1 cut(s) 813
EgeI GGCGCC 1 cut(s) 3439
EheI GGCGCC 1 cut(s) 3439
ErhI CCWWGG 2 cut(s) 686, 1606
Esp3I CGTCTC 2 cut(s) 1463, 3584
FalI AAGNNNNNCTT 2 cut(s) 858, 890
FaqI GGGAC 6 cut(s) 1207, 1350, 1588, 2636, 2883, 3093
FbaI TGATCA 2 cut(s) 118, 2403
FblI GTMKAC 2 cut(s) 1122, 1558
Fnu4HI GCNGC 3 cut(s) 456, 2596, 3241
FokI GGATG 7 cut(s) 179, 349, 515, 1700, 2170, 3416, 3570
FriOI GRGCYC 1 cut(s) 813
Fsp4HI GCNGC 3 cut(s) 456, 2596, 3241
FspBI CTAG 7 cut(s) 62, 479, 563, 884, 1134, 1727, 2504
GlaI GCGC 3 cut(s) 3439, 3498, 3629
GluI GCNGC 3 cut(s) 456, 2596, 3241
GsaI CCCAGC 1 cut(s) 3387
GsuI CTGGAG 5 cut(s) 841, 1398, 2004, 2465, 3386
HaeII RGCGCY 1 cut(s) 3441
HaeIII GGCC 5 cut(s) 26, 134, 2609, 3453, 3607
HapII CCGG 5 cut(s) 1685, 3120, 3197, 3292, 3502
HgaI GACGC 3 cut(s) 653, 1352, 3120
HhaI GCGC 3 cut(s) 3440, 3499, 3630
Hin1I GRCGYC 3 cut(s) 645, 1363, 3438
Hin6I GCGC 3 cut(s) 3438, 3497, 3628
HinP1I GCGC 3 cut(s) 3438, 3497, 3628
HincII GTYRAC 2 cut(s) 628, 966
HindII GTYRAC 2 cut(s) 628, 966
HindIII AAGCTT 5 cut(s) 698, 1236, 1972, 2546, 2747
HpaI GTTAAC 1 cut(s) 628
HpaII CCGG 5 cut(s) 1685, 3120, 3197, 3292, 3502
HphI GGTGA 9 cut(s) 104, 113, 1219, 1674, 2010, 2723, 3062, 3292, 3608
Hpy166II GTNNAC 9 cut(s) 628, 966, 1123, 1532, 1559, 1769, 1813, 2120, 3521
Hpy8I GTNNAC 9 cut(s) 628, 966, 1123, 1532, 1559, 1769, 1813, 2120, 3521
Hpy99I CGWCG 2 cut(s) 290, 3341
HpyCH4IV ACGT 4 cut(s) 72, 1411, 1429, 2327
HpyF10VI GCNNNNNNNGC 4 cut(s) 479, 697, 2719, 3373
HpySE526I ACGT 4 cut(s) 72, 1411, 1429, 2327
Hsp92I GRCGYC 3 cut(s) 645, 1363, 3438
HspAI GCGC 3 cut(s) 3438, 3497, 3628
KasI GGCGCC 1 cut(s) 3437
Kpn2I TCCGGA 1 cut(s) 3196
Ksp22I TGATCA 2 cut(s) 118, 2403
KspAI GTTAAC 1 cut(s) 628
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 7 cut(s) 696, 860, 1379, 2727, 3364, 3373, 3504
Lsp1109I GCAGC 2 cut(s) 467, 2607
LweI GCATC 5 cut(s) 1120, 2439, 2475, 3221, 3548
MaeI CTAG 7 cut(s) 62, 479, 563, 884, 1134, 1727, 2504
MaeII ACGT 4 cut(s) 72, 1411, 1429, 2327
MflI RGATCY 7 cut(s) 38, 475, 1200, 1729, 1831, 2987, 3405
MhlI GDGCHC 6 cut(s) 516, 582, 813, 1384, 2211, 3298
MlsI TGGCCA 1 cut(s) 3453
MluNI TGGCCA 1 cut(s) 3453
Mly113I GGCGCC 1 cut(s) 3438
MlyI GAGTC 6 cut(s) 527, 833, 1229, 1382, 2032, 2287
MmeI TCCRAC 4 cut(s) 1329, 1852, 2749, 3025
Mox20I TGGCCA 1 cut(s) 3453
Mph1103I ATGCAT 1 cut(s) 1950
MroI TCCGGA 1 cut(s) 3196
MroXI GAANNNNTTC 3 cut(s) 870, 1301, 2668
MscI TGGCCA 1 cut(s) 3453
MslI CAYNNNNRTG 4 cut(s) 1215, 1466, 1711, 2149
Msp20I TGGCCA 1 cut(s) 3453
MspA1I CMGCKG 3 cut(s) 935, 2598, 3240
MspCI CTTAAG 3 cut(s) 875, 1357, 2636
MspI CCGG 5 cut(s) 1685, 3120, 3197, 3292, 3502
MspR9I CCNGG 5 cut(s) 859, 917, 2741, 2784, 3293
MvaI CCWGG 4 cut(s) 859, 917, 2741, 2784
MwoI GCNNNNNNNGC 4 cut(s) 479, 697, 2719, 3373
NarI GGCGCC 1 cut(s) 3438
NciI CCSGG 1 cut(s) 3293
NcoI CCATGG 1 cut(s) 686
NlaIV GGNNCC 6 cut(s) 281, 692, 2932, 3439, 3506, 3581
NmeAIII GCCGAG 1 cut(s) 673
NmuCI GTSAC 8 cut(s) 17, 228, 1402, 1664, 1694, 2363, 2948, 3318
NsiI ATGCAT 1 cut(s) 1950
NspI RCATGY 2 cut(s) 1508, 1672
NspV TTCGAA 3 cut(s) 154, 254, 1570
PagI TCATGA 2 cut(s) 297, 2059
PaqCI CACCTGC 1 cut(s) 1511
PceI AGGCCT 2 cut(s) 26, 2609
PciI ACATGT 1 cut(s) 1668
PciSI GCTCTTC 1 cut(s) 198
PdmI GAANNNNTTC 3 cut(s) 870, 1301, 2668
PfeI GAWTC 9 cut(s) 550, 920, 1069, 1092, 2015, 2246, 2669, 3207, 3274
PflMI CCANNNNNTGG 1 cut(s) 3518
PfoI TCCNGGA 1 cut(s) 915
PkrI GCNGC 3 cut(s) 457, 2597, 3242
PleI GAGTC 6 cut(s) 526, 833, 1228, 1381, 2032, 2287
PluTI GGCGCC 1 cut(s) 3441
PmaCI CACGTG 1 cut(s) 1412
PmlI CACGTG 1 cut(s) 1412
PpsI GAGTC 6 cut(s) 526, 833, 1228, 1381, 2032, 2287
Ppu21I YACGTR 2 cut(s) 1412, 1430
PpuMI RGGWCCY 3 cut(s) 318, 566, 727
PscI ACATGT 1 cut(s) 1668
PsiI TTATAA 1 cut(s) 1341
Psp5II RGGWCCY 3 cut(s) 318, 566, 727
Psp6I CCWGG 4 cut(s) 857, 915, 2739, 2782
PspCI CACGTG 1 cut(s) 1412
PspFI CCCAGC 1 cut(s) 3383
PspGI CCWGG 4 cut(s) 857, 915, 2739, 2782
PspN4I GGNNCC 6 cut(s) 281, 692, 2932, 3439, 3506, 3581
PspPI GGNCC 5 cut(s) 133, 318, 566, 727, 3606
PspPPI RGGWCCY 3 cut(s) 318, 566, 727
PsrI GAACNNNNNNTAC 2 cut(s) 855, 887
PstI CTGCAG 1 cut(s) 2521
PstNI CAGNNNCTG 2 cut(s) 3243, 3277
PsuI RGATCY 7 cut(s) 38, 475, 1200, 1729, 1831, 2987, 3405
RsaI GTAC 6 cut(s) 804, 1531, 2080, 2225, 2276, 2326
RsaNI GTAC 6 cut(s) 803, 1530, 2079, 2224, 2275, 2325
RseI CAYNNNNRTG 4 cut(s) 1215, 1466, 1711, 2149
SapI GCTCTTC 1 cut(s) 198
SatI GCNGC 3 cut(s) 456, 2596, 3241
Sau96I GGNCC 5 cut(s) 133, 318, 566, 727, 3606
SchI GAGTC 6 cut(s) 527, 833, 1229, 1382, 2032, 2287
ScrFI CCNGG 5 cut(s) 859, 917, 2741, 2784, 3293
SduI GDGCHC 6 cut(s) 516, 582, 813, 1384, 2211, 3298
SfaNI GCATC 5 cut(s) 1120, 2439, 2475, 3221, 3548
SfcI CTRYAG 3 cut(s) 2427, 2517, 2694
SfoI GGCGCC 1 cut(s) 3439
SfuI TTCGAA 3 cut(s) 154, 254, 1570
SinI GGWCC 3 cut(s) 318, 566, 727
SmiMI CAYNNNNRTG 4 cut(s) 1215, 1466, 1711, 2149
SseBI AGGCCT 2 cut(s) 26, 2609
SsiI CCGC 5 cut(s) 933, 1089, 2598, 3240, 3359
SspDI GGCGCC 1 cut(s) 3437
SspI AATATT 4 cut(s) 1940, 2230, 2798, 3550
SspMI CTAG 7 cut(s) 62, 479, 563, 884, 1134, 1727, 2504
StuI AGGCCT 2 cut(s) 26, 2609
StyD4I CCNGG 5 cut(s) 857, 915, 2739, 2782, 3291
StyI CCWWGG 2 cut(s) 686, 1606
TaiI ACGT 4 cut(s) 75, 1414, 1432, 2330
TaqI TCGA 5 cut(s) 154, 254, 285, 1570, 2301
TauI GCSGC 1 cut(s) 3243
TfiI GAWTC 9 cut(s) 550, 920, 1069, 1092, 2015, 2246, 2669, 3207, 3274
TscAI CASTG 5 cut(s) 121, 552, 1669, 2017, 2541
TseFI GTSAC 8 cut(s) 17, 228, 1402, 1664, 1694, 2363, 2948, 3318
TseI GCWGC 2 cut(s) 455, 2595
Tsp45I GTSAC 8 cut(s) 17, 228, 1402, 1664, 1694, 2363, 2948, 3318
TspRI CASTG 5 cut(s) 121, 552, 1669, 2017, 2541
Van91I CCANNNNNTGG 1 cut(s) 3518
Vha464I CTTAAG 3 cut(s) 875, 1357, 2636
VpaK11BI GGWCC 3 cut(s) 318, 566, 727
XagI CCTNNNNNAGG 1 cut(s) 734
XapI RAATTY 9 cut(s) 490, 1184, 1284, 1352, 1790, 1988, 2936, 3005, 3182
XbaI TCTAGA 1 cut(s) 1726
XceI RCATGY 2 cut(s) 1508, 1672
XmiI GTMKAC 2 cut(s) 1122, 1558
XmnI GAANNNNTTC 3 cut(s) 870, 1301, 2668
XspI CTAG 7 cut(s) 62, 479, 563, 884, 1134, 1727, 2504
Zsp2I ATGCAT 1 cut(s) 1950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.