MD02G1225900.v1.1

Nucleolar GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005154
Physical Location & Seq
Reverse (-)
26503500 .. 26524577
21078 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1225900.v1.1.491

Sequence Viewer

Length: 1296 bp
ATGATGAGCAGGGCTTGCAGTCTCTTCCAATTGTGGCAGACCCCTAACAGATTACCACTCACACTCTCCAAAATTTCAAAAGGTCCTCACTTGATACCGAGTGCTAGAAATTATCCGGTTCTGTGCTTCTGCAAGGAAATACAAACTAGCACATACGAAGTTGTCAAGGGAAGCTATGTACCGTCAGCCGAAAAATCACCCGAAAATCTTGAAGATGTTGGTGCATTCCAAAAGCTACCCATGGTGATGCCATCTGTTGACATTCTGTATTCGGCACTAAGGAAGGCTAAGAGGATTACACCAACAAAGGGTATTGCCAATATTGCAAAGCGAGAAAGAAATAGAGGTTCAAAGCAACTTGATGCATTGATGAAAGAACTGGCAATTCCATTGAGAACTTATTCGGAGAATTTTCCAAATAAAAAGTGTCTTCACCCTTATGAACGATCTCTTATTGAATTAACTCTTGGGGATGGAAATTATGAACAGGTGTTGGGAAATGTGGATGCTTTGAGGAAGAAGGTTGTGTCTATTGGAAAGGAACATGCATCTCTTTGTGCTAAGTCAACGACGAAGAAGGAAGCAGAGGCAAGACTAACTGAGGGTATAAAGAAAGTTGAAGAGACATTTAATCGTGAAGGAAAACATGTTGATGATTTGCTACACATAGCCAAGACTCTGCGAGCAATGCCAGTAGTTGATCTGGAAGCACCAACACTTTGTCTTGTTGGAGCTCCTAATGTTGGGAAGTCATCTTTGGTTCGTATACTCTCCACTGGGAAGCCTGAGGTCTGCAACTACCCGTTCACAACAAGAGGAATACTAATGGGTCATATTATATCAAACCATCAAACTTTTCAGGTGACAGACACTCCTGGCCTGCTGAAGAGATGTGATGAGGACAGAAATAATTTGGAAAAATTGACACTTGCTGTCCTCTCACATTTACCGACCGCAATTCTCTATGTCCATGACCTCTCTGGGGAATGCGGGACCTCACCTTCTGACCAGTTTGTCATATACAAAGAAATAAGGGAGAGGTTTGGTGAACATTTGTGGCTTGATGTTGTGTCCAAATGTGATCTGTTGCAAAAGTCTCCTGTGCTATTTGCCACTGATAATGGTGATGATTCTGATCTTAAGTTGGAAAGGTACCGGAAATTGGGTCCCGATGGAGCTATTTATGTGTCAGTGACGAATGAAGTAGGGCTCACCGAGTTGAAGATTAGAGTGCATGAGATGCTAGATTCACAGATGAAGGATCCAAGTTCAAGAAAGCAACCAAGAAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1 NOG1

Protein Analysis

432

Amino Acids

48.22

Weight (kDa)

8.97

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOG1_N PF17835 76 - 234 2.7e-49 NOG1 N-terminal helical domain
FeoB_N PF02421 239 - 296 5.6e-09 Ferrous iron transport protein B
MMR_HSR1 PF01926 240 - 336 4.2e-16 50S ribosome-binding GTPase
NOG1 PF06858 305 - 361 1.5e-17 Nucleolar GTP-binding protein 1 (NOG1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1013
Acc65I GGTACC 1 cut(s) 1152
AccB1I GGYRCC 1 cut(s) 1152
AccI GTMKAC 1 cut(s) 766
AciI CCGC 2 cut(s) 954, 990
AclWI GGATC 2 cut(s) 1256, 1269
AcsI RAATTY 2 cut(s) 72, 409
AcuI CTGAAG 1 cut(s) 905
AfaI GTAC 2 cut(s) 180, 1154
AfiI CCNNNNNNNGG 4 cut(s) 308, 743, 982, 1162
AflII CTTAAG 1 cut(s) 1139
AflIII ACRYGT 1 cut(s) 646
AgsI TTSAA 7 cut(s) 78, 212, 351, 458, 620, 1222, 1272
AjnI CCWGG 1 cut(s) 874
AjuI GAANNNNNNNTTGG 4 cut(s) 450, 482, 740, 772
AluBI AGCT 5 cut(s) 174, 235, 734, 1178, 1292
AluI AGCT 5 cut(s) 174, 235, 734, 1178, 1292
Alw21I GWGCWC 1 cut(s) 736
Alw26I GTCTC 3 cut(s) 26, 617, 1101
AlwI GGATC 2 cut(s) 1256, 1269
AoxI GGCC 1 cut(s) 877
ApoI RAATTY 2 cut(s) 72, 409
Asp700I GAANNNNTTC 1 cut(s) 400
Asp718I GGTACC 1 cut(s) 1152
AspS9I GGNCC 3 cut(s) 83, 993, 1166
AsuHPI GGTGA 8 cut(s) 189, 256, 425, 874, 990, 1058, 1136, 1204
AvaII GGWCC 3 cut(s) 83, 993, 1166
AxyI CCTNAGG 1 cut(s) 786
BamHI GGATCC 1 cut(s) 1261
BanI GGYRCC 1 cut(s) 1152
BanII GRGCYC 2 cut(s) 736, 1212
BbsI GAAGAC 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 736
BccI CCATC 4 cut(s) 259, 467, 855, 1166
BciT130I CCWGG 1 cut(s) 876
BcoDI GTCTC 3 cut(s) 26, 617, 1101
BfaI CTAG 3 cut(s) 105, 147, 1244
BfrI CTTAAG 1 cut(s) 1139
Bme1390I CCNGG 1 cut(s) 876
Bme18I GGWCC 3 cut(s) 83, 993, 1166
BmgT120I GGNCC 3 cut(s) 83, 993, 1166
BmiI GGNNCC 5 cut(s) 994, 1154, 1167, 1168, 1263
BmrFI CCNGG 1 cut(s) 876
BmrI ACTGGG 1 cut(s) 786
BmsI GCATC 5 cut(s) 237, 352, 496, 557, 1230
BmuI ACTGGG 1 cut(s) 786
BpiI GAAGAC 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 1134
BsaJI CCNNGG 1 cut(s) 240
BsaWI WCCGGW 2 cut(s) 115, 1155
BsaXI ACNNNNNCTCC 2 cut(s) 856, 886
Bsc4I CCNNNNNNNGG 4 cut(s) 308, 743, 982, 1162
Bse1I ACTGG 4 cut(s) 384, 692, 781, 1009
Bse21I CCTNAGG 1 cut(s) 786
Bse3DI GCAATG 1 cut(s) 693
Bse8I GATNNNNATC 1 cut(s) 1134
BseBI CCWGG 1 cut(s) 876
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 2 cut(s) 478, 511
BseJI GATNNNNATC 1 cut(s) 1134
BseLI CCNNNNNNNGG 4 cut(s) 308, 743, 982, 1162
BseMI GCAATG 1 cut(s) 693
BseMII CTCAG 2 cut(s) 591, 777
BseNI ACTGG 4 cut(s) 384, 692, 781, 1009
Bsh1285I CGRYCG 1 cut(s) 954
BshFI GGCC 1 cut(s) 879
BshNI GGYRCC 1 cut(s) 1152
BsiEI CGRYCG 1 cut(s) 954
BsiHKAI GWGCWC 1 cut(s) 736
BsiSI CCGG 2 cut(s) 116, 1156
BslFI GGGAC 2 cut(s) 1006, 1152
BslI CCNNNNNNNGG 4 cut(s) 308, 743, 982, 1162
BsmAI GTCTC 3 cut(s) 26, 617, 1101
BsmFI GGGAC 2 cut(s) 1006, 1152
BsmI GAATGC 2 cut(s) 224, 992
BsnI GGCC 1 cut(s) 879
Bsp1286I GDGCHC 2 cut(s) 736, 1212
Bsp143I GATC 5 cut(s) 446, 700, 1081, 1135, 1261
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 2 cut(s) 954, 990
BspANI GGCC 1 cut(s) 879
BspCNI CTCAG 2 cut(s) 592, 778
BspLI GGNNCC 5 cut(s) 994, 1154, 1167, 1168, 1263
BspPI GGATC 2 cut(s) 1256, 1269
BspT107I GGYRCC 1 cut(s) 1152
BspTI CTTAAG 1 cut(s) 1139
BsrDI GCAATG 1 cut(s) 693
BsrI ACTGG 4 cut(s) 384, 692, 781, 1009
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 5 cut(s) 446, 700, 1081, 1135, 1261
BssNAI GTATAC 1 cut(s) 767
BssT1I CCWWGG 1 cut(s) 240
Bst1107I GTATAC 1 cut(s) 767
Bst2UI CCWGG 1 cut(s) 876
Bst4CI ACNGT 1 cut(s) 183
Bst6I CTCTTC 3 cut(s) 29, 615, 881
BstAFI CTTAAG 1 cut(s) 1139
BstAPI GCANNNNNTGC 2 cut(s) 15, 1240
BstC8I GCNNGC 3 cut(s) 16, 684, 881
BstDEI CTNAG 5 cut(s) 278, 288, 561, 600, 786
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 2 cut(s) 478, 511
BstKTI GATC 5 cut(s) 449, 703, 1084, 1138, 1264
BstMAI GTCTC 3 cut(s) 26, 617, 1101
BstMBI GATC 5 cut(s) 446, 700, 1081, 1135, 1261
BstMCI CGRYCG 1 cut(s) 954
BstMWI GCNNNNNNNGC 4 cut(s) 15, 323, 688, 1240
BstNI CCWGG 1 cut(s) 876
BstNSI RCATGY 2 cut(s) 548, 650
BstSCI CCNGG 1 cut(s) 874
BstV2I GAAGAC 1 cut(s) 422
BstX2I RGATCY 1 cut(s) 1261
BstYI RGATCY 1 cut(s) 1261
BstZ17I GTATAC 1 cut(s) 767
Bsu36I CCTNAGG 1 cut(s) 786
BsuRI GGCC 1 cut(s) 879
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 2 cut(s) 478, 511
BtsIMutI CAGTG 3 cut(s) 774, 1113, 1197
Cac8I GCNNGC 3 cut(s) 16, 684, 881
Cfr13I GGNCC 3 cut(s) 83, 993, 1166
Csp6I GTAC 2 cut(s) 179, 1153
CviAII CATG 5 cut(s) 241, 545, 647, 971, 1235
CviQI GTAC 2 cut(s) 179, 1153
DdeI CTNAG 5 cut(s) 278, 288, 561, 600, 786
DpnI GATC 5 cut(s) 448, 702, 1083, 1137, 1263
DpnII GATC 5 cut(s) 446, 700, 1081, 1135, 1261
DrdI GACNNNNNNGTC 1 cut(s) 1013
DseDI GACNNNNNNGTC 1 cut(s) 1013
Eam1104I CTCTTC 3 cut(s) 29, 615, 881
EarI CTCTTC 3 cut(s) 29, 615, 881
Ecl136II GAGCTC 1 cut(s) 734
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 2 cut(s) 736, 1212
Eco47I GGWCC 3 cut(s) 83, 993, 1166
Eco53kI GAGCTC 1 cut(s) 734
Eco57I CTGAAG 1 cut(s) 905
Eco81I CCTNAGG 1 cut(s) 786
EcoICRI GAGCTC 1 cut(s) 734
EcoO109I RGGNCCY 3 cut(s) 83, 993, 1166
EcoRII CCWGG 1 cut(s) 874
EcoT14I CCWWGG 1 cut(s) 240
EcoT22I ATGCAT 2 cut(s) 367, 550
EcoT38I GRGCYC 2 cut(s) 736, 1212
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 5 cut(s) 244, 548, 650, 974, 1238
FalI AAGNNNNNCTT 1 cut(s) 1276
FaqI GGGAC 2 cut(s) 1006, 1152
FatI CATG 5 cut(s) 240, 544, 646, 970, 1234
FauI CCCGC 1 cut(s) 983
FblI GTMKAC 1 cut(s) 766
FokI GGATG 2 cut(s) 485, 518
FriOI GRGCYC 2 cut(s) 736, 1212
FspBI CTAG 3 cut(s) 105, 147, 1244
HaeIII GGCC 1 cut(s) 879
HapII CCGG 2 cut(s) 116, 1156
Hin1II CATG 5 cut(s) 244, 548, 650, 974, 1238
HincII GTYRAC 2 cut(s) 259, 567
HindII GTYRAC 2 cut(s) 259, 567
HindIII AAGCTT 1 cut(s) 1290
HinfI GANTC 3 cut(s) 676, 1130, 1247
HpaII CCGG 2 cut(s) 116, 1156
HphI GGTGA 8 cut(s) 189, 256, 425, 874, 990, 1058, 1136, 1204
Hpy166II GTNNAC 5 cut(s) 259, 567, 767, 807, 1049
Hpy188I TCNGA 3 cut(s) 406, 1006, 1135
Hpy188III TCNNGA 5 cut(s) 209, 635, 704, 1169, 1272
Hpy8I GTNNAC 5 cut(s) 259, 567, 767, 807, 1049
Hpy99I CGWCG 1 cut(s) 574
HpyAV CCTTC 6 cut(s) 277, 514, 571, 632, 1011, 1252
HpyCH4III ACNGT 1 cut(s) 183
HpyCH4V TGCA 9 cut(s) 18, 132, 224, 326, 365, 548, 795, 1090, 1234
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 323, 688, 1240
HpyF3I CTNAG 5 cut(s) 278, 288, 561, 600, 786
Hsp92II CATG 5 cut(s) 244, 548, 650, 974, 1238
KflI GGGWCCC 1 cut(s) 1166
KpnI GGTACC 1 cut(s) 1156
Kzo9I GATC 5 cut(s) 446, 700, 1081, 1135, 1261
LmnI GCTCC 3 cut(s) 731, 739, 1175
LweI GCATC 5 cut(s) 237, 352, 496, 557, 1230
MaeI CTAG 3 cut(s) 105, 147, 1244
MaeIII GTNAC 2 cut(s) 862, 1192
MalI GATC 5 cut(s) 448, 702, 1083, 1137, 1263
MboI GATC 5 cut(s) 446, 700, 1081, 1135, 1261
MboII GAAGA 8 cut(s) 16, 224, 422, 529, 586, 632, 898, 1234
MfeI CAATTG 1 cut(s) 29
MflI RGATCY 1 cut(s) 1261
MhlI GDGCHC 2 cut(s) 736, 1212
MlyI GAGTC 1 cut(s) 670
MmeI TCCRAC 2 cut(s) 709, 1125
Mph1103I ATGCAT 2 cut(s) 367, 550
MroXI GAANNNNTTC 1 cut(s) 400
MseI TTAA 3 cut(s) 461, 630, 1140
MslI CAYNNNNRTG 3 cut(s) 245, 438, 651
MspCI CTTAAG 1 cut(s) 1139
MspI CCGG 2 cut(s) 116, 1156
MspR9I CCNGG 1 cut(s) 876
MunI CAATTG 1 cut(s) 29
Mva1269I GAATGC 2 cut(s) 224, 992
MvaI CCWGG 1 cut(s) 876
MwoI GCNNNNNNNGC 4 cut(s) 15, 323, 688, 1240
NcoI CCATGG 1 cut(s) 240
NdeII GATC 5 cut(s) 446, 700, 1081, 1135, 1261
NlaIII CATG 5 cut(s) 244, 548, 650, 974, 1238
NlaIV GGNNCC 5 cut(s) 994, 1154, 1167, 1168, 1263
NmuCI GTSAC 2 cut(s) 862, 1192
NsiI ATGCAT 2 cut(s) 367, 550
NspI RCATGY 2 cut(s) 548, 650
PciI ACATGT 1 cut(s) 646
PctI GAATGC 2 cut(s) 224, 992
PdmI GAANNNNTTC 1 cut(s) 400
PfeI GAWTC 2 cut(s) 1130, 1247
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
PpuMI RGGWCCY 3 cut(s) 83, 993, 1166
PscI ACATGT 1 cut(s) 646
Psp124BI GAGCTC 1 cut(s) 736
Psp5II RGGWCCY 3 cut(s) 83, 993, 1166
Psp6I CCWGG 1 cut(s) 874
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 5 cut(s) 994, 1154, 1167, 1168, 1263
PspPI GGNCC 3 cut(s) 83, 993, 1166
PspPPI RGGWCCY 3 cut(s) 83, 993, 1166
PsuI RGATCY 1 cut(s) 1261
RsaI GTAC 2 cut(s) 180, 1154
RsaNI GTAC 2 cut(s) 179, 1153
RseI CAYNNNNRTG 3 cut(s) 245, 438, 651
SacI GAGCTC 1 cut(s) 736
SaqAI TTAA 3 cut(s) 461, 630, 1140
Sau3AI GATC 5 cut(s) 446, 700, 1081, 1135, 1261
Sau96I GGNCC 3 cut(s) 83, 993, 1166
SchI GAGTC 1 cut(s) 670
ScrFI CCNGG 1 cut(s) 876
SduI GDGCHC 2 cut(s) 736, 1212
SfaNI GCATC 5 cut(s) 237, 352, 496, 557, 1230
SinI GGWCC 3 cut(s) 83, 993, 1166
SmiMI CAYNNNNRTG 3 cut(s) 245, 438, 651
SmlI CTYRAG 1 cut(s) 1139
SmoI CTYRAG 1 cut(s) 1139
SsiI CCGC 2 cut(s) 954, 990
SspI AATATT 1 cut(s) 322
SspMI CTAG 3 cut(s) 105, 147, 1244
SstI GAGCTC 1 cut(s) 736
StyD4I CCNGG 1 cut(s) 874
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 183
TfiI GAWTC 2 cut(s) 1130, 1247
Tru1I TTAA 3 cut(s) 461, 630, 1140
Tru9I TTAA 3 cut(s) 461, 630, 1140
TscAI CASTG 3 cut(s) 781, 1120, 1197
TseFI GTSAC 2 cut(s) 862, 1192
Tsp45I GTSAC 2 cut(s) 862, 1192
TspDTI ATGAA 5 cut(s) 386, 456, 498, 1215, 1271
TspRI CASTG 3 cut(s) 781, 1120, 1197
Vha464I CTTAAG 1 cut(s) 1139
VpaK11BI GGWCC 3 cut(s) 83, 993, 1166
XapI RAATTY 2 cut(s) 72, 409
XceI RCATGY 2 cut(s) 548, 650
XcmI CCANNNNNNNNNTGG 1 cut(s) 977
XmiI GTMKAC 1 cut(s) 766
XmnI GAANNNNTTC 1 cut(s) 400
XspI CTAG 3 cut(s) 105, 147, 1244
Zsp2I ATGCAT 2 cut(s) 367, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.