Rh6BG212200

Nucleolar GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005154
Physical Location & Seq
Reverse (-)
40026903 .. 40032591
5689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG212200.1

Sequence Viewer

Length: 774 bp
ATGGTAATGCCATCCGTTGACATTCTTTATTCAGCACTAAGGAAGGCTAAGAAAATTGCACCAACAAAAGGCATTGCCAATATTGCAAAGAGAGAAAGAAATAGAGGTGCAAAGCAACTTGATGCATTGATGAAAGAAATAGCAGTTCCTTTAAGGACTTATTGTGAGAACTTTCCCAATAAAAAGTATCTTCACCCTTATGAGCGATCTCTTATTGAATTAACTCTCGGGGATGGAAATTATGAACGGGTATTGGGAAAGGTGGATGCTCTGAGGAAAAATGTTGTGTCTGTTGGAAAGGAACATGCATCTCTATGTGCTAAGTCAACGTCAAAGAAGGAAGCAGAGACAAGATTGAGTGAGGGTTTAAAGAAAGTTGAGGAGACATATATTCGTCATGGAAAATATGTTGATGATTTGTTACACATAGCCAAGACATTGCGAGCAATGCCGGTAGTTGATCTAGAAACACCAACACTTTGTCTTGTTGGAGCTCCTAATGTTGGGAAGTCATCTTTGGTTCGTATACTCTCCACAGGGAAGCCTGAGGTCTGCAACTACCCTTTCACAACAAGAGGAATACTAATGGGTCATATCATTTTAAACCATGAGACTATTCAGGTGACTGATACTCCGGGCCTGCTGAAGAGATGTGATGAGGACAGAAATAATTTGGAAAAATTGACACTTGCTGTCCTCTCACATCTGCCAACTGCAGTACTGTATGTCCATGATCTTTCTGGAGAATGTGGGACCTCACCTACTGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1 NOG1

Protein Analysis

257

Amino Acids

28.5

Weight (kDa)

9.11

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOG1_N PF17835 1 - 154 4.9e-45 NOG1 N-terminal helical domain
FeoB_N PF02421 159 - 216 1.6e-09 Ferrous iron transport protein B
MMR_HSR1 PF01926 160 - 245 4.1e-16 50S ribosome-binding GTPase
NOG1 PF06858 225 - 257 4.3e-08 Nucleolar GTP-binding protein 1 (NOG1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 665
AfaI GTAC 1 cut(s) 720
AfiI CCNNNNNNNGG 2 cut(s) 68, 503
AgsI TTSAA 1 cut(s) 218
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AluBI AGCT 1 cut(s) 494
AluI AGCT 1 cut(s) 494
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 3 cut(s) 341, 377, 605
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 1 cut(s) 637
AspS9I GGNCC 2 cut(s) 637, 753
AsuC2I CCSGG 1 cut(s) 636
AsuHPI GGTGA 3 cut(s) 185, 634, 750
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 1 cut(s) 753
AxyI CCTNAGG 1 cut(s) 546
BanII GRGCYC 1 cut(s) 496
Bbv12I GWGCWC 1 cut(s) 496
BccI CCATC 2 cut(s) 19, 227
BclI TGATCA 1 cut(s) 766
BcnI CCSGG 1 cut(s) 636
BcoDI GTCTC 3 cut(s) 341, 377, 605
BfaI CTAG 1 cut(s) 464
BfmI CTRYAG 1 cut(s) 714
BmcAI AGTACT 1 cut(s) 720
Bme1390I CCNGG 1 cut(s) 636
Bme18I GGWCC 1 cut(s) 753
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 637, 753
BmiI GGNNCC 1 cut(s) 754
BmrFI CCNGG 1 cut(s) 636
BmsI GCATC 3 cut(s) 112, 256, 317
BpmI CTGGAG 1 cut(s) 762
BpuMI CCSGG 1 cut(s) 636
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 503
Bse118I RCCGGY 1 cut(s) 451
Bse21I CCTNAGG 1 cut(s) 546
Bse3DI GCAATG 3 cut(s) 72, 437, 453
BseGI GGATG 3 cut(s) 11, 238, 271
BseLI CCNNNNNNNGG 2 cut(s) 68, 503
BseMI GCAATG 3 cut(s) 72, 437, 453
BseMII CTCAG 2 cut(s) 263, 537
BseRI GAGGAG 1 cut(s) 395
BshFI GGCC 1 cut(s) 639
BsiHKAI GWGCWC 1 cut(s) 496
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 2 cut(s) 452, 635
BslFI GGGAC 1 cut(s) 766
BslI CCNNNNNNNGG 2 cut(s) 68, 503
BsmAI GTCTC 3 cut(s) 341, 377, 605
BsmFI GGGAC 1 cut(s) 766
BsnI GGCC 1 cut(s) 639
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 496
Bsp143I GATC 4 cut(s) 206, 460, 733, 766
BspANI GGCC 1 cut(s) 639
BspCNI CTCAG 2 cut(s) 264, 538
BspLI GGNNCC 1 cut(s) 754
BspMAI CTGCAG 1 cut(s) 718
BsrDI GCAATG 3 cut(s) 72, 437, 453
BsrFI RCCGGY 1 cut(s) 451
BssAI RCCGGY 1 cut(s) 451
BssMI GATC 4 cut(s) 206, 460, 733, 766
BssNAI GTATAC 1 cut(s) 527
Bst1107I GTATAC 1 cut(s) 527
Bst4CI ACNGT 1 cut(s) 723
Bst6I CTCTTC 1 cut(s) 641
BstC8I GCNNGC 2 cut(s) 444, 641
BstDEI CTNAG 5 cut(s) 38, 48, 272, 321, 546
BstF5I GGATG 3 cut(s) 11, 238, 271
BstKTI GATC 4 cut(s) 209, 463, 736, 769
BstMAI GTCTC 3 cut(s) 341, 377, 605
BstMBI GATC 4 cut(s) 206, 460, 733, 766
BstMWI GCNNNNNNNGC 2 cut(s) 83, 448
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 634
BstSFI CTRYAG 1 cut(s) 714
BstZ17I GTATAC 1 cut(s) 527
Bsu36I CCTNAGG 1 cut(s) 546
BsuRI GGCC 1 cut(s) 639
BtsCI GGATG 3 cut(s) 11, 238, 271
Cac8I GCNNGC 2 cut(s) 444, 641
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 2 cut(s) 637, 753
Csp6I GTAC 1 cut(s) 719
CviAII CATG 4 cut(s) 305, 398, 608, 731
CviJI RGCY 5 cut(s) 47, 431, 494, 544, 639
CviKI_1 RGCY 5 cut(s) 47, 431, 494, 544, 639
CviQI GTAC 1 cut(s) 719
DdeI CTNAG 5 cut(s) 38, 48, 272, 321, 546
DpnI GATC 4 cut(s) 208, 462, 735, 768
DpnII GATC 4 cut(s) 206, 460, 733, 766
DraI TTTAAA 2 cut(s) 369, 603
Eam1104I CTCTTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 641
Ecl136II GAGCTC 1 cut(s) 494
Eco24I GRGCYC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 753
Eco53kI GAGCTC 1 cut(s) 494
Eco57I CTGAAG 1 cut(s) 665
Eco81I CCTNAGG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 753
EcoT22I ATGCAT 2 cut(s) 127, 310
EcoT38I GRGCYC 1 cut(s) 496
FaeI CATG 4 cut(s) 308, 401, 611, 734
FaqI GGGAC 1 cut(s) 766
FatI CATG 4 cut(s) 304, 397, 607, 730
FbaI TGATCA 1 cut(s) 766
FblI GTMKAC 1 cut(s) 526
FokI GGATG 2 cut(s) 245, 278
FriOI GRGCYC 1 cut(s) 496
FspBI CTAG 1 cut(s) 464
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 1 cut(s) 639
HapII CCGG 2 cut(s) 452, 635
Hin1II CATG 4 cut(s) 308, 401, 611, 734
HincII GTYRAC 2 cut(s) 19, 327
HindII GTYRAC 2 cut(s) 19, 327
HpaII CCGG 2 cut(s) 452, 635
HphI GGTGA 3 cut(s) 185, 634, 750
Hpy166II GTNNAC 3 cut(s) 19, 327, 527
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 2 cut(s) 464, 741
Hpy8I GTNNAC 3 cut(s) 19, 327, 527
HpyAV CCTTC 2 cut(s) 37, 331
HpyCH4III ACNGT 1 cut(s) 723
HpyCH4IV ACGT 1 cut(s) 329
HpyCH4V TGCA 7 cut(s) 59, 86, 110, 125, 308, 555, 716
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 448
HpyF3I CTNAG 5 cut(s) 38, 48, 272, 321, 546
HpySE526I ACGT 1 cut(s) 329
Hsp92II CATG 4 cut(s) 308, 401, 611, 734
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 4 cut(s) 206, 460, 733, 766
LmnI GCTCC 2 cut(s) 491, 499
LpnPI CCDG 7 cut(s) 465, 522, 558, 605, 648, 653, 726
LweI GCATC 3 cut(s) 112, 256, 317
MaeI CTAG 1 cut(s) 464
MaeII ACGT 1 cut(s) 329
MaeIII GTNAC 2 cut(s) 420, 622
MalI GATC 4 cut(s) 208, 462, 735, 768
MboI GATC 4 cut(s) 206, 460, 733, 766
MboII GAAGA 2 cut(s) 182, 658
MhlI GDGCHC 1 cut(s) 496
MluCI AATT 5 cut(s) 54, 218, 238, 670, 680
MmeI TCCRAC 2 cut(s) 274, 469
MnlI CCTC 9 cut(s) 98, 267, 355, 373, 541, 569, 652, 707, 766
Mph1103I ATGCAT 2 cut(s) 127, 310
MseI TTAA 4 cut(s) 152, 221, 368, 602
MslI CAYNNNNRTG 2 cut(s) 198, 313
MspI CCGG 2 cut(s) 452, 635
MspR9I CCNGG 1 cut(s) 636
MwoI GCNNNNNNNGC 2 cut(s) 83, 448
NciI CCSGG 1 cut(s) 636
NdeII GATC 4 cut(s) 206, 460, 733, 766
NlaIII CATG 4 cut(s) 308, 401, 611, 734
NlaIV GGNNCC 1 cut(s) 754
NmuCI GTSAC 1 cut(s) 622
NsiI ATGCAT 2 cut(s) 127, 310
NspI RCATGY 1 cut(s) 308
PpuMI RGGWCCY 1 cut(s) 753
Psp124BI GAGCTC 1 cut(s) 496
Psp5II RGGWCCY 1 cut(s) 753
PspN4I GGNNCC 1 cut(s) 754
PspPI GGNCC 2 cut(s) 637, 753
PspPPI RGGWCCY 1 cut(s) 753
PstI CTGCAG 1 cut(s) 718
RsaI GTAC 1 cut(s) 720
RsaNI GTAC 1 cut(s) 719
RseI CAYNNNNRTG 2 cut(s) 198, 313
SacI GAGCTC 1 cut(s) 496
SaqAI TTAA 4 cut(s) 152, 221, 368, 602
Sau3AI GATC 4 cut(s) 206, 460, 733, 766
Sau96I GGNCC 2 cut(s) 637, 753
ScaI AGTACT 1 cut(s) 720
ScrFI CCNGG 1 cut(s) 636
SduI GDGCHC 1 cut(s) 496
SetI ASST 8 cut(s) 109, 264, 332, 496, 552, 624, 758, 763
SfaNI GCATC 3 cut(s) 112, 256, 317
SfcI CTRYAG 1 cut(s) 714
SinI GGWCC 1 cut(s) 753
SmiMI CAYNNNNRTG 2 cut(s) 198, 313
Sse9I AATT 5 cut(s) 54, 218, 238, 670, 680
SspI AATATT 1 cut(s) 82
SspMI CTAG 1 cut(s) 464
SstI GAGCTC 1 cut(s) 496
StyD4I CCNGG 1 cut(s) 634
TaaI ACNGT 1 cut(s) 723
TaiI ACGT 1 cut(s) 332
TasI AATT 5 cut(s) 54, 218, 238, 670, 680
TatI WGTACW 1 cut(s) 718
Tru1I TTAA 4 cut(s) 152, 221, 368, 602
Tru9I TTAA 4 cut(s) 152, 221, 368, 602
TseFI GTSAC 1 cut(s) 622
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 2 cut(s) 146, 258
TspGWI ACGGA 1 cut(s) 4
VpaK11BI GGWCC 1 cut(s) 753
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 1 cut(s) 308
XcmI CCANNNNNNNNNTGG 1 cut(s) 737
XmiI GTMKAC 1 cut(s) 526
XspI CTAG 1 cut(s) 464
ZrmI AGTACT 1 cut(s) 720
Zsp2I ATGCAT 2 cut(s) 127, 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.