FvH4_7g08680

Nucleolar GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005154
Physical Location & Seq
Forward (+)
8501274 .. 8509955
8682 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08680.t2

Sequence Viewer

Length: 1380 bp
ATGAGCAGGGCTTGCAGTCTCTTTCAGCTATGGCACAGTCCCCAAGACACCAGATTATTACTCTCCATCTCCAAAGGGAAATACTTGACACCTAGTGCCAGAAGTTATCCGGGTCTGTGCTTCTGTAAGGGTTTACAGACTTCTGCATACGAAGTTGTCAAGGGAAGCTATGTACCCACTGCCCAGATTAAACAAGAAAGCAAAACTGAGGAAAAATCTCCGGAGATACTTGAAGGTGTAGGTGCATTTCAAAAGCTACCAATGGTAATGCCATCTGTTGACATTCTGTATTCAGCACTAAGGAAGGCTAAGAAAGTTGCACCAACAAAAGGCATTGCCAATATTGCAAAGAGAGAAAGAAATAGAGGTGCAAAGCAACTTGATGCATTGATGAAAGAAATAGCAGTTCCGTTGAGGACTTATTCTGAGAAGTTTCCCAATAAAAAGTATCTTCACCCTTATGAGCGATCTCTTATCGAATTAACTCTCGGGGATGGAAATTATGAACAGGTGTTGGGAAAGGTGGATGCTCTGAGGAAAAAGGTTGTGTCTGTTGGCAAGGAACATGCATCTCTTTGTGCAAAGTCAACGTCGAAGAAGGAAGCAGAGACAAGACTGAGTGAGGGTTTAAAGAAAGTTGAAGAGACATATATTCGACAAGGAAAATATGTTGATGATTTGTTACACATAGCCAAGACATTGCGAGCAATGCCCGTAGTTGATCTAGAAACGCCAACACTTTGTCTTGTTGGAGCTCCTAATGTTGGAAAGTCATCTTTGGTCCGTATACTCTCCACAGGGAAGCCTGAGGTCTGCAACTACCCTTTCACAACAAGAGGAATACTTATGGGTCATATCATTTTAAACCATGAGACTTTTCAGGTGACCGATACACCAGGTCTGCTGAAGAGATGTGATGAGGACCGAAATAATTTGGAAAAGTTGACACTTGCTGTCCTCTCACATCTACCAACTGCAGTACTGTATGTCCATGATCTTTCTGGCGAATGTGGGATGTCACCTTCCGATCAGTTTGTCATTTACAATGAAATAAAGGAAAAATTTGGTGACCATCTGTGGCTAGATGTTGTGTCGAAATGTGATCTGTTGCAAGAGTCTCCAGTGATATTTGCTACTGATAATGACGATGATAATGATCTTAGCCTGGAAAGGTACCTGAAGTTGGGCCCTGAGGGAGCTCTTCATGTGTCAGTGAAAAATGAAATAGGGCTCCCAGAGTTGAAAAGAAGAGTGCATGAACTGCTGAATACCCAAATGAGCATGATCCAGAAAAGAAGAGTGCATGAACCGCTGAATACCCAAATGACCAGGTTCCAAAGTCAAGAAACCAACCAAGAAACTCTTCAAGTTCTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1 NOG1

Protein Analysis

460

Amino Acids

51.54

Weight (kDa)

8.74

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOG1_N PF17835 83 - 241 8.1e-48 NOG1 N-terminal helical domain
FeoB_N PF02421 246 - 303 5.4e-09 Ferrous iron transport protein B
MMR_HSR1 PF01926 247 - 345 3.8e-16 50S ribosome-binding GTPase
NOG1 PF06858 312 - 368 1.3e-17 Nucleolar GTP-binding protein 1 (NOG1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1173
AccB1I GGYRCC 1 cut(s) 1173
AccI GTMKAC 1 cut(s) 787
AccIII TCCGGA 1 cut(s) 220
AciI CCGC 1 cut(s) 1310
AclWI GGATC 1 cut(s) 1279
AcsI RAATTY 1 cut(s) 1061
AcuI CTGAAG 2 cut(s) 926, 1199
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 3 cut(s) 174, 981, 1175
AfiI CCNNNNNNNGG 3 cut(s) 329, 764, 1183
AgsI TTSAA 5 cut(s) 233, 251, 641, 1243, 1367
AjnI CCWGG 3 cut(s) 895, 1164, 1328
AjuI GAANNNNNNNTTGG 2 cut(s) 1347, 1379
AluBI AGCT 5 cut(s) 28, 168, 256, 755, 1199
AluI AGCT 5 cut(s) 28, 168, 256, 755, 1199
Alw21I GWGCWC 2 cut(s) 757, 1201
Alw26I GTCTC 5 cut(s) 23, 602, 638, 866, 1122
AlwI GGATC 1 cut(s) 1279
Ama87I CYCGRG 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 220
AoxI GGCC 1 cut(s) 1186
ApaI GGGCCC 1 cut(s) 1190
ApoI RAATTY 1 cut(s) 1061
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
Asp700I GAANNNNTTC 1 cut(s) 1362
Asp718I GGTACC 1 cut(s) 1173
AspS9I GGNCC 4 cut(s) 781, 922, 1186, 1187
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 4 cut(s) 446, 895, 1011, 1079
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 2 cut(s) 781, 922
AxyI CCTNAGG 2 cut(s) 807, 1191
BaeGI GKGCMC 1 cut(s) 1190
BanI GGYRCC 1 cut(s) 1173
BanII GRGCYC 4 cut(s) 757, 1190, 1201, 1233
Bbv12I GWGCWC 2 cut(s) 757, 1201
BccI CCATC 4 cut(s) 74, 280, 488, 1080
BciT130I CCWGG 3 cut(s) 897, 1166, 1330
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 5 cut(s) 23, 602, 638, 866, 1122
BfaI CTAG 3 cut(s) 93, 725, 1082
BfmI CTRYAG 1 cut(s) 975
BmcAI AGTACT 1 cut(s) 981
Bme1390I CCNGG 4 cut(s) 111, 897, 1166, 1330
Bme18I GGWCC 2 cut(s) 781, 922
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 4 cut(s) 781, 922, 1186, 1187
BmiI GGNNCC 4 cut(s) 1175, 1188, 1232, 1334
BmrFI CCNGG 4 cut(s) 111, 897, 1166, 1330
BmsI GCATC 3 cut(s) 373, 517, 578
BpmI CTGGAG 1 cut(s) 1104
BpuMI CCSGG 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 1155
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 764, 1183
Bse1I ACTGG 1 cut(s) 1121
Bse21I CCTNAGG 2 cut(s) 807, 1191
Bse3DI GCAATG 3 cut(s) 333, 698, 714
Bse8I GATNNNNATC 1 cut(s) 1155
BseAI TCCGGA 1 cut(s) 220
BseBI CCWGG 3 cut(s) 897, 1166, 1330
BseGI GGATG 3 cut(s) 499, 532, 1020
BseJI GATNNNNATC 1 cut(s) 1155
BseLI CCNNNNNNNGG 3 cut(s) 329, 764, 1183
BseMI GCAATG 3 cut(s) 333, 698, 714
BseMII CTCAG 6 cut(s) 198, 417, 524, 608, 798, 1182
BseNI ACTGG 1 cut(s) 1121
BseSI GKGCMC 1 cut(s) 1190
BshFI GGCC 1 cut(s) 1188
BshNI GGYRCC 1 cut(s) 1173
BsiHKAI GWGCWC 2 cut(s) 757, 1201
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 2 cut(s) 110, 221
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 3 cut(s) 329, 764, 1183
BsmAI GTCTC 5 cut(s) 23, 602, 638, 866, 1122
BsmFI GGGAC 1 cut(s) 24
BsnI GGCC 1 cut(s) 1188
BsoBI CYCGRG 1 cut(s) 488
Bsp120I GGGCCC 1 cut(s) 1186
Bsp1286I GDGCHC 4 cut(s) 757, 1190, 1201, 1233
Bsp13I TCCGGA 1 cut(s) 220
Bsp143I GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
BspACI CCGC 1 cut(s) 1310
BspANI GGCC 1 cut(s) 1188
BspCNI CTCAG 6 cut(s) 199, 418, 525, 609, 799, 1183
BspEI TCCGGA 1 cut(s) 220
BspLI GGNNCC 4 cut(s) 1175, 1188, 1232, 1334
BspMAI CTGCAG 1 cut(s) 979
BspPI GGATC 1 cut(s) 1279
BspQI GCTCTTC 1 cut(s) 1206
BspT107I GGYRCC 1 cut(s) 1173
BsrDI GCAATG 3 cut(s) 333, 698, 714
BsrI ACTGG 1 cut(s) 1121
BssMI GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
BssNAI GTATAC 1 cut(s) 788
Bst1107I GTATAC 1 cut(s) 788
Bst2UI CCWGG 3 cut(s) 897, 1166, 1330
Bst4CI ACNGT 2 cut(s) 38, 984
Bst6I CTCTTC 6 cut(s) 636, 902, 1206, 1243, 1291, 1368
BstAPI GCANNNNNTGC 2 cut(s) 12, 1261
BstC8I GCNNGC 2 cut(s) 13, 705
BstDEI CTNAG 9 cut(s) 207, 299, 309, 426, 533, 617, 807, 1160, 1191
BstEII GGTNACC 2 cut(s) 883, 1067
BstF5I GGATG 3 cut(s) 499, 532, 1020
BstKTI GATC 7 cut(s) 470, 724, 997, 1030, 1105, 1159, 1287
BstMAI GTCTC 5 cut(s) 23, 602, 638, 866, 1122
BstMBI GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
BstMWI GCNNNNNNNGC 5 cut(s) 12, 344, 709, 1261, 1309
BstNI CCWGG 3 cut(s) 897, 1166, 1330
BstNSI RCATGY 1 cut(s) 569
BstPI GGTNACC 2 cut(s) 883, 1067
BstSCI CCNGG 4 cut(s) 109, 895, 1164, 1328
BstSFI CTRYAG 1 cut(s) 975
BstSLI GKGCMC 1 cut(s) 1190
BstZ17I GTATAC 1 cut(s) 788
Bsu36I CCTNAGG 2 cut(s) 807, 1191
BsuRI GGCC 1 cut(s) 1188
BtsCI GGATG 3 cut(s) 499, 532, 1020
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 3 cut(s) 177, 1128, 1218
Cac8I GCNNGC 2 cut(s) 13, 705
Cfr13I GGNCC 4 cut(s) 781, 922, 1186, 1187
CsiI ACCWGGT 2 cut(s) 895, 1328
Csp6I GTAC 3 cut(s) 173, 980, 1174
CviAII CATG 7 cut(s) 566, 869, 992, 1205, 1256, 1282, 1304
CviQI GTAC 3 cut(s) 173, 980, 1174
DdeI CTNAG 9 cut(s) 207, 299, 309, 426, 533, 617, 807, 1160, 1191
DpnI GATC 7 cut(s) 469, 723, 996, 1029, 1104, 1158, 1286
DpnII GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
DraI TTTAAA 2 cut(s) 630, 864
DraIII CACNNNGTG 1 cut(s) 95
Eam1104I CTCTTC 6 cut(s) 636, 902, 1206, 1243, 1291, 1368
EarI CTCTTC 6 cut(s) 636, 902, 1206, 1243, 1291, 1368
Ecl136II GAGCTC 2 cut(s) 755, 1199
Eco24I GRGCYC 4 cut(s) 757, 1190, 1201, 1233
Eco47I GGWCC 2 cut(s) 781, 922
Eco53kI GAGCTC 2 cut(s) 755, 1199
Eco57I CTGAAG 2 cut(s) 926, 1199
Eco81I CCTNAGG 2 cut(s) 807, 1191
Eco88I CYCGRG 1 cut(s) 488
Eco91I GGTNACC 2 cut(s) 883, 1067
EcoICRI GAGCTC 2 cut(s) 755, 1199
EcoO109I RGGNCCY 1 cut(s) 1187
EcoO65I GGTNACC 2 cut(s) 883, 1067
EcoRII CCWGG 3 cut(s) 895, 1164, 1328
EcoT22I ATGCAT 2 cut(s) 388, 571
EcoT38I GRGCYC 4 cut(s) 757, 1190, 1201, 1233
FaeI CATG 7 cut(s) 569, 872, 995, 1208, 1259, 1285, 1307
FalI AAGNNNNNCTT 2 cut(s) 1347, 1379
FaqI GGGAC 1 cut(s) 24
FatI CATG 7 cut(s) 565, 868, 991, 1204, 1255, 1281, 1303
FblI GTMKAC 1 cut(s) 787
FokI GGATG 3 cut(s) 506, 539, 1027
FriOI GRGCYC 4 cut(s) 757, 1190, 1201, 1233
FspBI CTAG 3 cut(s) 93, 725, 1082
GsuI CTGGAG 1 cut(s) 1104
HaeIII GGCC 1 cut(s) 1188
HapII CCGG 2 cut(s) 110, 221
Hin1II CATG 7 cut(s) 569, 872, 995, 1208, 1259, 1285, 1307
HincII GTYRAC 3 cut(s) 280, 588, 945
HindII GTYRAC 3 cut(s) 280, 588, 945
HinfI GANTC 1 cut(s) 1115
HpaII CCGG 2 cut(s) 110, 221
HphI GGTGA 4 cut(s) 446, 895, 1011, 1079
Hpy166II GTNNAC 5 cut(s) 134, 280, 588, 788, 945
Hpy188I TCNGA 3 cut(s) 427, 534, 1027
Hpy188III TCNNGA 4 cut(s) 221, 725, 1288, 1343
Hpy8I GTNNAC 5 cut(s) 134, 280, 588, 788, 945
Hpy99I CGWCG 1 cut(s) 595
HpyAV CCTTC 4 cut(s) 227, 298, 592, 1032
HpyCH4III ACNGT 2 cut(s) 38, 984
HpyCH4IV ACGT 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 344, 709, 1261, 1309
HpyF3I CTNAG 9 cut(s) 207, 299, 309, 426, 533, 617, 807, 1160, 1191
HpySE526I ACGT 1 cut(s) 590
Hsp92II CATG 7 cut(s) 569, 872, 995, 1208, 1259, 1285, 1307
Kpn2I TCCGGA 1 cut(s) 220
KpnI GGTACC 1 cut(s) 1177
Kzo9I GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 4 cut(s) 752, 760, 1196, 1236
LweI GCATC 3 cut(s) 373, 517, 578
MabI ACCWGGT 2 cut(s) 895, 1328
MaeI CTAG 3 cut(s) 93, 725, 1082
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 4 cut(s) 681, 883, 1017, 1067
MalI GATC 7 cut(s) 469, 723, 996, 1029, 1104, 1158, 1286
MboI GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
MboII GAAGA 8 cut(s) 443, 607, 653, 919, 1193, 1260, 1308, 1355
MhlI GDGCHC 4 cut(s) 757, 1190, 1201, 1233
MluCI AATT 4 cut(s) 479, 499, 931, 1061
MlyI GAGTC 1 cut(s) 1124
MmeI TCCRAC 2 cut(s) 730, 745
Mph1103I ATGCAT 2 cut(s) 388, 571
MroI TCCGGA 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 1362
MseI TTAA 4 cut(s) 189, 482, 629, 863
MslI CAYNNNNRTG 1 cut(s) 459
MspA1I CMGCKG 1 cut(s) 1312
MspI CCGG 2 cut(s) 110, 221
MspR9I CCNGG 4 cut(s) 111, 897, 1166, 1330
MvaI CCWGG 3 cut(s) 897, 1166, 1330
MwoI GCNNNNNNNGC 5 cut(s) 12, 344, 709, 1261, 1309
NciI CCSGG 1 cut(s) 111
NdeII GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
NlaIII CATG 7 cut(s) 569, 872, 995, 1208, 1259, 1285, 1307
NlaIV GGNNCC 4 cut(s) 1175, 1188, 1232, 1334
NmuCI GTSAC 3 cut(s) 883, 1017, 1067
NsiI ATGCAT 2 cut(s) 388, 571
NspI RCATGY 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 1206
PdmI GAANNNNTTC 1 cut(s) 1362
PleI GAGTC 1 cut(s) 1123
PpsI GAGTC 1 cut(s) 1123
Psp124BI GAGCTC 2 cut(s) 757, 1201
Psp6I CCWGG 3 cut(s) 895, 1164, 1328
PspEI GGTNACC 2 cut(s) 883, 1067
PspGI CCWGG 3 cut(s) 895, 1164, 1328
PspN4I GGNNCC 4 cut(s) 1175, 1188, 1232, 1334
PspOMI GGGCCC 1 cut(s) 1186
PspPI GGNCC 4 cut(s) 781, 922, 1186, 1187
PstI CTGCAG 1 cut(s) 979
RsaI GTAC 3 cut(s) 174, 981, 1175
RsaNI GTAC 3 cut(s) 173, 980, 1174
RseI CAYNNNNRTG 1 cut(s) 459
SacI GAGCTC 2 cut(s) 757, 1201
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 4 cut(s) 189, 482, 629, 863
Sau3AI GATC 7 cut(s) 467, 721, 994, 1027, 1102, 1156, 1284
Sau96I GGNCC 4 cut(s) 781, 922, 1186, 1187
ScaI AGTACT 1 cut(s) 981
SchI GAGTC 1 cut(s) 1124
ScrFI CCNGG 4 cut(s) 111, 897, 1166, 1330
SduI GDGCHC 4 cut(s) 757, 1190, 1201, 1233
SexAI ACCWGGT 2 cut(s) 895, 1328
SfaNI GCATC 3 cut(s) 373, 517, 578
SfcI CTRYAG 1 cut(s) 975
SinI GGWCC 2 cut(s) 781, 922
SmiMI CAYNNNNRTG 1 cut(s) 459
Sse9I AATT 4 cut(s) 479, 499, 931, 1061
SsiI CCGC 1 cut(s) 1310
SspI AATATT 1 cut(s) 343
SspMI CTAG 3 cut(s) 93, 725, 1082
SstI GAGCTC 2 cut(s) 757, 1201
StyD4I CCNGG 4 cut(s) 109, 895, 1164, 1328
TaaI ACNGT 2 cut(s) 38, 984
TaiI ACGT 1 cut(s) 593
TaqI TCGA 4 cut(s) 477, 593, 655, 1094
TaqII GACCGA 2 cut(s) 902, 939
TasI AATT 4 cut(s) 479, 499, 931, 1061
TatI WGTACW 1 cut(s) 979
Tru1I TTAA 4 cut(s) 189, 482, 629, 863
Tru9I TTAA 4 cut(s) 189, 482, 629, 863
TscAI CASTG 3 cut(s) 184, 1128, 1218
TseFI GTSAC 3 cut(s) 883, 1017, 1067
Tsp45I GTSAC 3 cut(s) 883, 1017, 1067
TspDTI ATGAA 7 cut(s) 407, 519, 1062, 1193, 1236, 1272, 1320
TspGWI ACGGA 2 cut(s) 399, 773
TspRI CASTG 3 cut(s) 184, 1128, 1218
VpaK11BI GGWCC 2 cut(s) 781, 922
XapI RAATTY 1 cut(s) 1061
XbaI TCTAGA 1 cut(s) 724
XceI RCATGY 1 cut(s) 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 998
XmiI GTMKAC 1 cut(s) 787
XmnI GAANNNNTTC 1 cut(s) 1362
XspI CTAG 3 cut(s) 93, 725, 1082
ZrmI AGTACT 1 cut(s) 981
Zsp2I ATGCAT 2 cut(s) 388, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.