AT2G03350

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004492
Physical Location & Seq
Reverse (-)
1019535 .. 1021186
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G03350.1

Sequence Viewer

Length: 540 bp
ATGGAGAAGGCATTAACGAAAGTGACGAGCTTTAAAGTGGGAAGTTCATGGATCTCTAAGAAAGCTAAAGAAGAGCTCTCAAACATCACCAATGACCTCACTACATTCTCCAGTACGGTTGAAGAGAAAGCAAAATGGATTTTCAACAAGCTTAAAGGAAAACCACTGAAAAGCTTACCAGACCTCCTCAAAGAATACAACTTACCACCAGGACTCTTCCCACAAAACATAATCTGCTACGAATTCGATGAGACGAAGAATAAACTCACCGTCTTCTTCTCTTCACCATGTGAAGTCACATTCAAAGATGGATCAGCGATAAGATATGCAACGCGTGTGAAAGGGATTTTGCTTAGAGGGAAGCTGATGGGTGTGGAAGGAATGAAGACAAAAGTGTTGGTGTGGGTTAAAGTCACAACAATCTCAGTCGAGAGCTCCAAGTCTGACAAGCTCTGGTTCACTGCTGGTGTCAAGAAATCTCGATCTAAGGATGTTTATGACACACCTCATGATGCTATCAAAGTCATTGGAGAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

179

Amino Acids

20.17

Weight (kDa)

9.57

Isoelectric Point (pI)

27.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 61 - 166 3.7e-29 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 334
AclWI GGATC 2 cut(s) 59, 319
AcsI RAATTY 1 cut(s) 242
AdeI CACNNNGTG 1 cut(s) 290
AfaI GTAC 1 cut(s) 115
AflIII ACRYGT 1 cut(s) 332
AgsI TTSAA 3 cut(s) 122, 145, 304
AjnI CCWGG 1 cut(s) 208
AluBI AGCT 8 cut(s) 30, 65, 76, 151, 174, 364, 435, 451
AluI AGCT 8 cut(s) 30, 65, 76, 151, 174, 364, 435, 451
Alw21I GWGCWC 2 cut(s) 78, 437
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 2 cut(s) 59, 319
ApoI RAATTY 1 cut(s) 242
AsuHPI GGTGA 3 cut(s) 79, 259, 276
BanII GRGCYC 2 cut(s) 78, 437
BbsI GAAGAC 2 cut(s) 265, 392
Bbv12I GWGCWC 2 cut(s) 78, 437
BccI CCATC 2 cut(s) 302, 361
BciT130I CCWGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 245
Bme1390I CCNGG 1 cut(s) 210
BmrFI CCNGG 1 cut(s) 210
BmsI GCATC 1 cut(s) 502
BpiI GAAGAC 2 cut(s) 265, 392
BpmI CTGGAG 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bse1I ACTGG 1 cut(s) 111
BseBI CCWGG 1 cut(s) 210
BseGI GGATG 1 cut(s) 496
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 1 cut(s) 111
BseRI GAGGAG 1 cut(s) 176
Bsh1236I CGCG 1 cut(s) 334
BsiHKAI GWGCWC 2 cut(s) 78, 437
BsmAI GTCTC 1 cut(s) 245
BsmBI CGTCTC 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 78, 437
Bsp143I GATC 3 cut(s) 51, 311, 482
BspCNI CTCAG 1 cut(s) 437
BspFNI CGCG 1 cut(s) 334
BspHI TCATGA 1 cut(s) 508
BspPI GGATC 2 cut(s) 59, 319
BspQI GCTCTTC 1 cut(s) 66
BsrI ACTGG 1 cut(s) 111
BssMI GATC 3 cut(s) 51, 311, 482
Bst2UI CCWGG 1 cut(s) 210
Bst4CI ACNGT 2 cut(s) 118, 271
Bst6I CTCTTC 4 cut(s) 66, 117, 221, 286
BstDEI CTNAG 4 cut(s) 57, 353, 424, 486
BstF5I GGATG 1 cut(s) 496
BstFNI CGCG 1 cut(s) 334
BstKTI GATC 3 cut(s) 54, 314, 485
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 3 cut(s) 51, 311, 482
BstNI CCWGG 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 208
BstUI CGCG 1 cut(s) 334
BstV2I GAAGAC 2 cut(s) 265, 392
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 2 cut(s) 164, 459
CciI TCATGA 1 cut(s) 508
Csp6I GTAC 1 cut(s) 114
CviAII CATG 3 cut(s) 48, 288, 509
CviJI RGCY 8 cut(s) 30, 65, 76, 151, 174, 364, 435, 451
CviKI_1 RGCY 8 cut(s) 30, 65, 76, 151, 174, 364, 435, 451
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 4 cut(s) 57, 353, 424, 486
DpnI GATC 3 cut(s) 53, 313, 484
DpnII GATC 3 cut(s) 51, 311, 482
DraI TTTAAA 1 cut(s) 34
DraIII CACNNNGTG 1 cut(s) 290
Eam1104I CTCTTC 4 cut(s) 66, 117, 221, 286
EarI CTCTTC 4 cut(s) 66, 117, 221, 286
Ecl136II GAGCTC 2 cut(s) 76, 435
Eco24I GRGCYC 2 cut(s) 78, 437
Eco53kI GAGCTC 2 cut(s) 76, 435
EcoICRI GAGCTC 2 cut(s) 76, 435
EcoRI GAATTC 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 208
EcoT38I GRGCYC 2 cut(s) 78, 437
Esp3I CGTCTC 1 cut(s) 245
FaeI CATG 3 cut(s) 51, 291, 512
FaiI YATR 6 cut(s) 49, 230, 289, 327, 498, 510
FatI CATG 3 cut(s) 47, 287, 508
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 2 cut(s) 78, 437
GsuI CTGGAG 1 cut(s) 94
Hin1II CATG 3 cut(s) 51, 291, 512
HindIII AAGCTT 2 cut(s) 149, 172
HinfI GANTC 1 cut(s) 213
HphI GGTGA 3 cut(s) 79, 259, 276
Hpy166II GTNNAC 1 cut(s) 459
Hpy188I TCNGA 1 cut(s) 445
Hpy188III TCNNGA 4 cut(s) 430, 472, 480, 509
Hpy8I GTNNAC 1 cut(s) 459
HpyAV CCTTC 1 cut(s) 371
HpyCH4III ACNGT 2 cut(s) 118, 271
HpyCH4V TGCA 1 cut(s) 329
HpyF3I CTNAG 4 cut(s) 57, 353, 424, 486
Hsp92II CATG 3 cut(s) 51, 291, 512
Kzo9I GATC 3 cut(s) 51, 311, 482
LguI GCTCTTC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 440
LpnPI CCDG 6 cut(s) 124, 192, 195, 222, 439, 450
LweI GCATC 1 cut(s) 502
MaeIII GTNAC 3 cut(s) 22, 295, 412
MalI GATC 3 cut(s) 53, 313, 484
MboI GATC 3 cut(s) 51, 311, 482
MboII GAAGA 8 cut(s) 83, 134, 208, 265, 268, 268, 273, 397
MflI RGATCY 1 cut(s) 51
MhlI GDGCHC 2 cut(s) 78, 437
MluCI AATT 1 cut(s) 242
MluI ACGCGT 1 cut(s) 332
MlyI GAGTC 1 cut(s) 207
MnlI CCTC 5 cut(s) 107, 194, 197, 350, 516
MseI TTAA 4 cut(s) 14, 33, 153, 408
MspR9I CCNGG 1 cut(s) 210
MvaI CCWGG 1 cut(s) 210
MvnI CGCG 1 cut(s) 334
NdeII GATC 3 cut(s) 51, 311, 482
NlaIII CATG 3 cut(s) 51, 291, 512
NmuCI GTSAC 3 cut(s) 22, 295, 412
PagI TCATGA 1 cut(s) 508
PciSI GCTCTTC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
Psp124BI GAGCTC 2 cut(s) 78, 437
Psp6I CCWGG 1 cut(s) 208
PspGI CCWGG 1 cut(s) 208
PsuI RGATCY 1 cut(s) 51
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SacI GAGCTC 2 cut(s) 78, 437
SapI GCTCTTC 1 cut(s) 66
SaqAI TTAA 4 cut(s) 14, 33, 153, 408
Sau3AI GATC 3 cut(s) 51, 311, 482
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 1 cut(s) 210
SduI GDGCHC 2 cut(s) 78, 437
SfaNI GCATC 1 cut(s) 502
Sse9I AATT 1 cut(s) 242
SstI GAGCTC 2 cut(s) 78, 437
StyD4I CCNGG 1 cut(s) 208
TaaI ACNGT 2 cut(s) 118, 271
TaqI TCGA 3 cut(s) 246, 429, 481
TasI AATT 1 cut(s) 242
Tru1I TTAA 4 cut(s) 14, 33, 153, 408
Tru9I TTAA 4 cut(s) 14, 33, 153, 408
TscAI CASTG 2 cut(s) 171, 466
TseFI GTSAC 3 cut(s) 22, 295, 412
Tsp45I GTSAC 3 cut(s) 22, 295, 412
TspDTI ATGAA 2 cut(s) 36, 398
TspRI CASTG 2 cut(s) 171, 466
XapI RAATTY 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.